AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38572


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38572.t1 MTR_3g099750 89.529 191 19 1 1 190 13 203 4.08e-119 342
MS.gene38572.t1 MTR_3g099740 85.340 191 27 1 1 190 14 204 1.08e-111 323
MS.gene38572.t1 MTR_5g065080 55.497 191 79 2 1 190 17 202 1.23e-59 190
MS.gene38572.t1 MTR_2g437590 36.316 190 105 2 2 189 67 242 1.26e-35 129
MS.gene38572.t1 MTR_3g058660 36.066 183 97 5 12 188 23 191 1.70e-24 98.6
MS.gene38572.t1 MTR_2g015470 31.892 185 116 2 4 188 42 216 2.55e-23 95.9
MS.gene38572.t1 MTR_2g437580 33.333 195 105 3 2 189 47 223 1.78e-22 93.6
MS.gene38572.t1 MTR_4g098900 34.783 184 101 5 11 187 42 213 9.70e-21 89.0
MS.gene38572.t1 MTR_7g080750 30.055 183 100 4 4 185 29 184 5.02e-19 83.6
MS.gene38572.t1 MTR_8g024620 28.495 186 116 2 5 190 43 211 1.66e-18 82.0
MS.gene38572.t1 MTR_8g024620 28.495 186 116 2 5 190 54 222 1.83e-18 82.0
MS.gene38572.t1 MTR_8g024620 28.495 186 116 2 5 190 55 223 1.84e-18 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38572.t1 AT1G28220 51.053 190 84 1 1 190 1 181 9.55e-64 201
MS.gene38572.t1 AT1G28230 51.579 190 89 1 1 190 1 187 2.31e-55 179
MS.gene38572.t1 AT2G33750 48.947 190 88 1 1 190 3 183 3.58e-55 178
MS.gene38572.t1 AT2G33750 48.947 190 88 1 1 190 3 183 6.25e-55 178
MS.gene38572.t1 AT4G18205 37.278 169 95 3 20 188 56 213 9.64e-25 99.8
MS.gene38572.t1 AT4G18195 33.511 188 113 4 2 188 43 219 5.80e-24 97.8
MS.gene38572.t1 AT4G18220 36.416 173 97 4 16 188 57 216 1.94e-23 96.3
MS.gene38572.t1 AT1G30840 35.135 185 107 3 5 189 27 198 3.20e-23 95.5
MS.gene38572.t1 AT1G30840 35.135 185 107 3 5 189 27 198 3.20e-23 95.5
MS.gene38572.t1 AT4G18210 34.682 173 100 3 16 188 57 216 7.65e-20 86.3
MS.gene38572.t1 AT4G18197 36.686 169 96 3 20 188 59 216 5.23e-18 81.3
MS.gene38572.t1 AT1G44750 29.050 179 113 1 5 183 33 197 1.87e-17 79.3
MS.gene38572.t1 AT1G44750 29.050 179 113 1 5 183 33 197 1.87e-17 79.3
MS.gene38572.t1 AT1G44750 30.726 179 110 1 5 183 45 209 2.14e-17 79.3
MS.gene38572.t1 AT4G18190 34.254 181 104 4 12 188 44 213 9.16e-17 77.4
MS.gene38572.t1 AT4G08700 28.070 171 106 2 20 190 52 205 3.23e-15 73.2
MS.gene38572.t1 AT1G44750 38.202 89 55 0 95 183 45 133 7.18e-15 71.6
MS.gene38572.t1 AT1G44750 38.202 89 55 0 95 183 45 133 7.18e-15 71.6
MS.gene38572.t1 AT1G44750 38.202 89 55 0 95 183 45 133 7.18e-15 71.6
MS.gene38572.t1 AT1G44750 38.202 89 55 0 95 183 45 133 7.18e-15 71.6
MS.gene38572.t1 AT1G44750 38.202 89 55 0 95 183 45 133 7.18e-15 71.6
MS.gene38572.t1 AT5G41160 27.219 169 109 2 22 190 54 208 6.34e-14 69.3

Find 55 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACGGTTGGTTCTGGTGTTT+TGG 0.295285 3.2:+82793239 MS.gene38572:CDS
AAGCTGAGATGCTATTATTA+AGG 0.319246 3.2:-82793139 None:intergenic
TGGTGGATGGTGGAGGATGG+TGG 0.364115 3.2:-82792975 None:intergenic
TCCACGGCGGCCAACGTGTC+TGG 0.372039 3.2:+82792869 MS.gene38572:CDS
AATGAGCATCAAGGGGAAAC+TGG 0.387179 3.2:-82792914 None:intergenic
TCAAGGGGAAACTGGCGGTT+TGG 0.389802 3.2:-82792906 None:intergenic
ATGGAGATGGTGGTGGATGG+TGG 0.398618 3.2:-82792985 None:intergenic
ATTAAGGAACCAGTGGAAAC+TGG 0.398731 3.2:-82793123 None:intergenic
TCTTCGCATATGGCGTTGCT+CGG 0.418830 3.2:+82793097 MS.gene38572:CDS
TGGTGGTGGATGGTGGAGGA+TGG 0.424033 3.2:-82792978 None:intergenic
TTCTTCGCAGCCGCCTTCAT+CGG 0.427701 3.2:+82793048 MS.gene38572:CDS
TGGATGGTGGAGGATGGTGG+TGG 0.430332 3.2:-82792972 None:intergenic
TTCTGTGATGGAGATGGTGG+TGG 0.432880 3.2:-82792992 None:intergenic
GCTCGGCTTCCAGTTTCCAC+TGG 0.458963 3.2:+82793114 MS.gene38572:CDS
GAAGAAAATAGAAGGTTTCA+TGG 0.460569 3.2:-82793031 None:intergenic
GGGGAGCACCGGAGGTACCT+AGG 0.461356 3.2:-82792825 None:intergenic
GACGACTATCTCTTCGCATA+TGG 0.467374 3.2:+82793087 MS.gene38572:CDS
GTGGAGGATGGTGGTGGAGA+CGG 0.467478 3.2:-82792966 None:intergenic
TGAAATAGAGACGCATTATG+AGG 0.471366 3.2:-82792846 None:intergenic
GCCGGTGAGGATTCCGATGA+AGG 0.474028 3.2:-82793061 None:intergenic
GAGATGGTGGTGGATGGTGG+AGG 0.481262 3.2:-82792982 None:intergenic
AGGGGAAACTGGCGGTTTGG+AGG 0.487139 3.2:-82792903 None:intergenic
GTGATGGAGATGGTGGTGGA+TGG 0.491847 3.2:-82792988 None:intergenic
GAAACTGGCGGTTTGGAGGA+AGG 0.498472 3.2:-82792899 None:intergenic
GCGGCTGCGAAGAAAATAGA+AGG 0.499053 3.2:-82793039 None:intergenic
GGGAGCACCGGAGGTACCTA+GGG 0.520001 3.2:-82792824 None:intergenic
TGCGAGGGGAATGAGCATCA+AGG 0.523525 3.2:-82792923 None:intergenic
ACGAGTAGTGATCGTCCTGC+TGG 0.534270 3.2:+82793270 MS.gene38572:CDS
GCCAGACACGTTGGCCGCCG+TGG 0.541479 3.2:-82792870 None:intergenic
ACGCATTATGAGGGGAGCAC+CGG 0.545670 3.2:-82792836 None:intergenic
GAAATAGAGACGCATTATGA+GGG 0.548591 3.2:-82792845 None:intergenic
TTAATAATAGCATCTCAGCT+TGG 0.557663 3.2:+82793141 MS.gene38572:CDS
ATGTTAGCCCTAGGTACCTC+CGG 0.565369 3.2:+82792817 MS.gene38572:CDS
TGCTATTATTAAGGAACCAG+TGG 0.565701 3.2:-82793130 None:intergenic
AATTGTCTCATGTTAGCCCT+AGG 0.571570 3.2:+82792808 MS.gene38572:CDS
GCGAGGGGAATGAGCATCAA+GGG 0.578405 3.2:-82792922 None:intergenic
GCCTTCATCGGAATCCTCAC+CGG 0.585533 3.2:+82793060 MS.gene38572:CDS
TGAGACAATTTACTATCAGA+AGG 0.586602 3.2:-82792795 None:intergenic
CATTATGAGGGGAGCACCGG+AGG 0.606065 3.2:-82792833 None:intergenic
ATGCGTCTCTATTTCATCCA+CGG 0.610236 3.2:+82792853 MS.gene38572:CDS
GAAGAGATAGTCGTCAAGGC+CGG 0.619969 3.2:-82793079 None:intergenic
GTATGATGTATGAGATTGCG+AGG 0.623652 3.2:-82792939 None:intergenic
ATGCGAAGAGATAGTCGTCA+AGG 0.632876 3.2:-82793083 None:intergenic
TATGATGTATGAGATTGCGA+GGG 0.643247 3.2:-82792938 None:intergenic
CAATGCAGAAGCAGCAACCG+TGG 0.648575 3.2:-82793328 None:intergenic
GGTGAGGATTCCGATGAAGG+CGG 0.655496 3.2:-82793058 None:intergenic
ACCAACCGTAAGCAAAACAA+TGG 0.657982 3.2:-82793226 None:intergenic
CGAGGGGAATGAGCATCAAG+GGG 0.659390 3.2:-82792921 None:intergenic
ACTGGCGGTTTGGAGGAAGG+CGG 0.661208 3.2:-82792896 None:intergenic
AAATAGAGACGCATTATGAG+GGG 0.661247 3.2:-82792844 None:intergenic
AGGCGGAGAGCCAGACACGT+TGG 0.665988 3.2:-82792879 None:intergenic
GATAGTCGTCAAGGCCGGTG+AGG 0.690358 3.2:-82793074 None:intergenic
GAGCATCAAGGGGAAACTGG+CGG 0.694601 3.2:-82792911 None:intergenic
ATGATGTATGAGATTGCGAG+GGG 0.708086 3.2:-82792937 None:intergenic
CGTCTCTATTTCATCCACGG+CGG 0.772458 3.2:+82792856 MS.gene38572:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTAGTTTTTCTGTGA+TGG - chr3.2:82793007-82793026 None:intergenic 20.0%
! ATAACAAGAGAATAAGTGAT+GGG - chr3.2:82793405-82793424 None:intergenic 25.0%
AAGCTGAGATGCTATTATTA+AGG - chr3.2:82793142-82793161 None:intergenic 30.0%
CATAACAAGAGAATAAGTGA+TGG - chr3.2:82793406-82793425 None:intergenic 30.0%
CATCACTTATTCTCTTGTTA+TGG + chr3.2:82793404-82793423 MS.gene38572:intron 30.0%
GAAGAAAATAGAAGGTTTCA+TGG - chr3.2:82793034-82793053 None:intergenic 30.0%
TGAGACAATTTACTATCAGA+AGG - chr3.2:82792798-82792817 None:intergenic 30.0%
TTAATAATAGCATCTCAGCT+TGG + chr3.2:82793141-82793160 MS.gene38572:CDS 30.0%
AAATAGAGACGCATTATGAG+GGG - chr3.2:82792847-82792866 None:intergenic 35.0%
AAGAAGAAAAGCGAAAAACG+CGG - chr3.2:82793172-82793191 None:intergenic 35.0%
GAAATAGAGACGCATTATGA+GGG - chr3.2:82792848-82792867 None:intergenic 35.0%
TATGATGTATGAGATTGCGA+GGG - chr3.2:82792941-82792960 None:intergenic 35.0%
TGAAATAGAGACGCATTATG+AGG - chr3.2:82792849-82792868 None:intergenic 35.0%
TGCTATTATTAAGGAACCAG+TGG - chr3.2:82793133-82793152 None:intergenic 35.0%
TTATAAACCAACTCAACCGA+AGG - chr3.2:82793369-82793388 None:intergenic 35.0%
!! AAACGCCATTGTTTTGCTTA+CGG + chr3.2:82793221-82793240 MS.gene38572:CDS 35.0%
!! TAGTTTTTCTGTGATGGAGA+TGG - chr3.2:82793001-82793020 None:intergenic 35.0%
!!! GTATGGATTTGTTTTGCCTT+CGG + chr3.2:82793350-82793369 MS.gene38572:intron 35.0%
!!! TGCGATTAGTTTTTCAACCA+CGG + chr3.2:82793311-82793330 MS.gene38572:CDS 35.0%
AATTGTCTCATGTTAGCCCT+AGG + chr3.2:82792808-82792827 MS.gene38572:CDS 40.0%
ACCAACCGTAAGCAAAACAA+TGG - chr3.2:82793229-82793248 None:intergenic 40.0%
ATGATGTATGAGATTGCGAG+GGG - chr3.2:82792940-82792959 None:intergenic 40.0%
ATGCGTCTCTATTTCATCCA+CGG + chr3.2:82792853-82792872 MS.gene38572:CDS 40.0%
ATTAAGGAACCAGTGGAAAC+TGG - chr3.2:82793126-82793145 None:intergenic 40.0%
GTATGATGTATGAGATTGCG+AGG - chr3.2:82792942-82792961 None:intergenic 40.0%
!! TTTTTCTGTGATGGAGATGG+TGG - chr3.2:82792998-82793017 None:intergenic 40.0%
AATGAGCATCAAGGGGAAAC+TGG - chr3.2:82792917-82792936 None:intergenic 45.0%
ATGCGAAGAGATAGTCGTCA+AGG - chr3.2:82793086-82793105 None:intergenic 45.0%
GACGACTATCTCTTCGCATA+TGG + chr3.2:82793087-82793106 MS.gene38572:CDS 45.0%
! GTTGCTGCTTCTGCATTGTA+TGG + chr3.2:82793333-82793352 MS.gene38572:intron 45.0%
! TGTTTTGCAGAAACACCAGC+AGG - chr3.2:82793288-82793307 None:intergenic 45.0%
! TGTTTTGCCTTCGGTTGAGT+TGG + chr3.2:82793359-82793378 MS.gene38572:intron 45.0%
!! GCCATTGTTTTGCTTACGGT+TGG + chr3.2:82793225-82793244 MS.gene38572:CDS 45.0%
!! GTTTTGCTTACGGTTGGTTC+TGG + chr3.2:82793231-82793250 MS.gene38572:CDS 45.0%
!!! TACGGTTGGTTCTGGTGTTT+TGG + chr3.2:82793239-82793258 MS.gene38572:CDS 45.0%
ATGTTAGCCCTAGGTACCTC+CGG + chr3.2:82792817-82792836 MS.gene38572:CDS 50.0%
CGTCTCTATTTCATCCACGG+CGG + chr3.2:82792856-82792875 MS.gene38572:CDS 50.0%
GAAGAGATAGTCGTCAAGGC+CGG - chr3.2:82793082-82793101 None:intergenic 50.0%
GCCACTCTCTTCTGTGCTAT+AGG + chr3.2:82793453-82793472 MS.gene38572:intron 50.0%
GCCTATAGCACAGAAGAGAG+TGG - chr3.2:82793457-82793476 None:intergenic 50.0%
GCGGCTGCGAAGAAAATAGA+AGG - chr3.2:82793042-82793061 None:intergenic 50.0%
TCTTCGCATATGGCGTTGCT+CGG + chr3.2:82793097-82793116 MS.gene38572:CDS 50.0%
! TTCTGTGATGGAGATGGTGG+TGG - chr3.2:82792995-82793014 None:intergenic 50.0%
ACGAGTAGTGATCGTCCTGC+TGG + chr3.2:82793270-82793289 MS.gene38572:CDS 55.0%
CAATGCAGAAGCAGCAACCG+TGG - chr3.2:82793331-82793350 None:intergenic 55.0%
CGAGGGGAATGAGCATCAAG+GGG - chr3.2:82792924-82792943 None:intergenic 55.0%
GAGCATCAAGGGGAAACTGG+CGG - chr3.2:82792914-82792933 None:intergenic 55.0%
GCCTTCATCGGAATCCTCAC+CGG + chr3.2:82793060-82793079 MS.gene38572:CDS 55.0%
GCGAGGGGAATGAGCATCAA+GGG - chr3.2:82792925-82792944 None:intergenic 55.0%
GGTGAGGATTCCGATGAAGG+CGG - chr3.2:82793061-82793080 None:intergenic 55.0%
TCAAGGGGAAACTGGCGGTT+TGG - chr3.2:82792909-82792928 None:intergenic 55.0%
TGCGAGGGGAATGAGCATCA+AGG - chr3.2:82792926-82792945 None:intergenic 55.0%
TTCTTCGCAGCCGCCTTCAT+CGG + chr3.2:82793048-82793067 MS.gene38572:CDS 55.0%
! ACGCATTATGAGGGGAGCAC+CGG - chr3.2:82792839-82792858 None:intergenic 55.0%
! GAAACTGGCGGTTTGGAGGA+AGG - chr3.2:82792902-82792921 None:intergenic 55.0%
!! ATGGAGATGGTGGTGGATGG+TGG - chr3.2:82792988-82793007 None:intergenic 55.0%
!! GTGATGGAGATGGTGGTGGA+TGG - chr3.2:82792991-82793010 None:intergenic 55.0%
GATAGTCGTCAAGGCCGGTG+AGG - chr3.2:82793077-82793096 None:intergenic 60.0%
GCCGGTGAGGATTCCGATGA+AGG - chr3.2:82793064-82793083 None:intergenic 60.0%
GCTCGGCTTCCAGTTTCCAC+TGG + chr3.2:82793114-82793133 MS.gene38572:CDS 60.0%
! ACTGGCGGTTTGGAGGAAGG+CGG - chr3.2:82792899-82792918 None:intergenic 60.0%
! AGGGGAAACTGGCGGTTTGG+AGG - chr3.2:82792906-82792925 None:intergenic 60.0%
!! CATTATGAGGGGAGCACCGG+AGG - chr3.2:82792836-82792855 None:intergenic 60.0%
!! GAGATGGTGGTGGATGGTGG+AGG - chr3.2:82792985-82793004 None:intergenic 60.0%
!! GTGGAGGATGGTGGTGGAGA+CGG - chr3.2:82792969-82792988 None:intergenic 60.0%
!! TGGATGGTGGAGGATGGTGG+TGG - chr3.2:82792975-82792994 None:intergenic 60.0%
!! TGGTGGATGGTGGAGGATGG+TGG - chr3.2:82792978-82792997 None:intergenic 60.0%
!! TGGTGGTGGATGGTGGAGGA+TGG - chr3.2:82792981-82793000 None:intergenic 60.0%
AGGCGGAGAGCCAGACACGT+TGG - chr3.2:82792882-82792901 None:intergenic 65.0%
!! GGGAGCACCGGAGGTACCTA+GGG - chr3.2:82792827-82792846 None:intergenic 65.0%
TCCACGGCGGCCAACGTGTC+TGG + chr3.2:82792869-82792888 MS.gene38572:CDS 70.0%
!! GGGGAGCACCGGAGGTACCT+AGG - chr3.2:82792828-82792847 None:intergenic 70.0%
GCCAGACACGTTGGCCGCCG+TGG - chr3.2:82792873-82792892 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 82792784 82793493 82792784 ID=MS.gene38572
chr3.2 mRNA 82792784 82793493 82792784 ID=MS.gene38572.t1;Parent=MS.gene38572
chr3.2 exon 82792784 82793349 82792784 ID=MS.gene38572.t1.exon1;Parent=MS.gene38572.t1
chr3.2 CDS 82792784 82793349 82792784 ID=cds.MS.gene38572.t1;Parent=MS.gene38572.t1
chr3.2 exon 82793475 82793493 82793475 ID=MS.gene38572.t1.exon2;Parent=MS.gene38572.t1
chr3.2 CDS 82793475 82793493 82793475 ID=cds.MS.gene38572.t1;Parent=MS.gene38572.t1
Gene Sequence

>MS.gene38572

ATGAAGAGATTCCTTCTGATAGTAAATTGTCTCATGTTAGCCCTAGGTACCTCCGGTGCTCCCCTCATAATGCGTCTCTATTTCATCCACGGCGGCCAACGTGTCTGGCTCTCCGCCTTCCTCCAAACCGCCAGTTTCCCCTTGATGCTCATTCCCCTCGCAATCTCATACATCATACGCTACCGTCTCCACCACCATCCTCCACCATCCACCACCATCTCCATCACAGAAAAACTAAATATAATCTCCATGAAACCTTCTATTTTCTTCGCAGCCGCCTTCATCGGAATCCTCACCGGCCTTGACGACTATCTCTTCGCATATGGCGTTGCTCGGCTTCCAGTTTCCACTGGTTCCTTAATAATAGCATCTCAGCTTGGTTTCACCGCGTTTTTCGCTTTTCTTCTTGTGAAACAAAAATTTACAGCTTTTACTGTAAACGCCATTGTTTTGCTTACGGTTGGTTCTGGTGTTTTGGCTATGCATACGAGTAGTGATCGTCCTGCTGGTGTTTCTGCAAAACAATATGCGATTAGTTTTTCAACCACGGTTGCTGCTTCTGCATTGTATGGATTTGTTTTGCCTTCGGTTGAGTTGGTTTATAAGAAGATTAAACAACCCATCACTTATTCTCTTGTTATGGAATTTCAGTTCGTTATGTGCATGTTTGCCACTCTCTTCTGTGCTATAGGCATGATTGCAAACAATGA

Protein sequence

>MS.gene38572.t1

MKRFLLIVNCLMLALGTSGAPLIMRLYFIHGGQRVWLSAFLQTASFPLMLIPLAISYIIRYRLHHHPPPSTTISITEKLNIISMKPSIFFAAAFIGILTGLDDYLFAYGVARLPVSTGSLIIASQLGFTAFFAFLLVKQKFTAFTVNAIVLLTVGSGVLAMHTSSDRPAGVSAKQYAISFSTTVAASALHDCKQ