AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055629


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055629.t1 MTR_4g064963 93.333 165 8 1 1 162 1 165 9.19e-109 307
MS.gene055629.t1 MTR_4g065660 77.852 149 30 3 14 162 8 153 1.05e-67 202
MS.gene055629.t1 MTR_4g065123 72.662 139 36 2 12 150 1 137 1.51e-58 179
MS.gene055629.t1 MTR_7g066070 45.139 144 72 2 14 156 2 139 4.53e-38 127
MS.gene055629.t1 MTR_4g019870 47.899 119 58 1 40 154 13 131 4.26e-36 122
MS.gene055629.t1 MTR_0027s0220 51.240 121 53 4 21 138 11 128 1.46e-29 105
MS.gene055629.t1 MTR_1g015000 32.639 144 72 4 39 162 1 139 1.24e-16 72.4
MS.gene055629.t1 MTR_3g105330 29.078 141 73 3 39 156 1 137 2.92e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055629.t1 AT2G26975 50.340 147 67 3 12 156 1 143 3.15e-42 138
MS.gene055629.t1 AT5G59030 50.806 124 57 2 43 162 45 168 1.88e-35 121
MS.gene055629.t1 AT3G46900 48.333 120 60 2 44 161 34 153 2.57e-34 118
MS.gene055629.t1 AT5G59040 43.651 126 71 0 34 159 24 149 1.42e-32 113
MS.gene055629.t1 AT2G37925 48.544 103 50 1 39 138 29 131 2.63e-29 105
MS.gene055629.t1 AT5G20650 34.400 125 57 1 39 138 1 125 9.05e-15 67.8

Find 42 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGGTTGGGTTAGCTTATT+TGG 0.161485 4.4:+41390734 MS.gene055629:CDS
ACCCATCATCATGGTGTTAT+TGG 0.242191 4.4:-41390490 None:intergenic
GTTGATGATGATGATGGTTT+TGG 0.274914 4.4:-41390468 None:intergenic
TCTTGTGTAGCAGTGCTATT+AGG 0.286924 4.4:+41390829 MS.gene055629:CDS
TGATGCACATGACATTCTTC+TGG 0.300847 4.4:+41390529 MS.gene055629:CDS
TAAGCCTTATCATCATGTTT+TGG 0.336110 4.4:-41390855 None:intergenic
CTTTAATTCTCTTCAACAAT+TGG 0.349322 4.4:+41390562 MS.gene055629:CDS
GCTGTGCTTGGTCATGCTCT+TGG 0.350764 4.4:+41390801 MS.gene055629:CDS
TTGGCTCTTATGTCTTTCAA+TGG 0.387989 4.4:+41390762 MS.gene055629:CDS
GTTAGCTTATTTGGTTATGT+TGG 0.388968 4.4:+41390743 MS.gene055629:CDS
GGCAGCAACATGGTTCGAAC+CGG 0.422987 4.4:-41390682 None:intergenic
GAAACCAAAACATGATGATA+AGG 0.457740 4.4:+41390851 MS.gene055629:CDS
GATGCACATGACATTCTTCT+GGG 0.472072 4.4:+41390530 MS.gene055629:CDS
AACCAATAACACCATGATGA+TGG 0.477184 4.4:+41390488 MS.gene055629:CDS
CTCTTCAACAATTGGCCTAA+CGG 0.488088 4.4:+41390570 MS.gene055629:CDS
ATGCACGGTGGCGGCATGGA+TGG 0.492115 4.4:+41390432 MS.gene055629:CDS
AATTGGCCTAACGGTGATAC+CGG 0.503435 4.4:+41390579 MS.gene055629:CDS
CAAAAGTGTCTGAAACAAAC+CGG 0.510839 4.4:-41390703 None:intergenic
GGTTCGAACCATGTTGCTGC+CGG 0.517508 4.4:+41390684 MS.gene055629:CDS
TTATTGGTTGATGATGATGA+TGG 0.527547 4.4:-41390474 None:intergenic
TGATACCGGTATGTATGTAT+TGG 0.532754 4.4:+41390593 MS.gene055629:CDS
ACCAATAACACCATGATGAT+GGG 0.534019 4.4:+41390489 MS.gene055629:CDS
GGTGGTAACATGCACGGTGG+CGG 0.534398 4.4:+41390423 MS.gene055629:CDS
GCTCTTATGTCTTTCAATGG+TGG 0.539076 4.4:+41390765 MS.gene055629:CDS
GATCTCACATCCATGGATCA+TGG 0.541471 4.4:+41390402 None:intergenic
TGAGAGTAATTCAATGAGTA+CGG 0.561035 4.4:-41390640 None:intergenic
GCAGCAACATGGTTCGAACC+GGG 0.567283 4.4:-41390681 None:intergenic
CATGTTACCACCATGATCCA+TGG 0.570126 4.4:-41390412 None:intergenic
CATCTTATGACCCATCATCA+TGG 0.576922 4.4:-41390499 None:intergenic
TTGAATTACTCTCACGTACA+CGG 0.584631 4.4:+41390649 MS.gene055629:CDS
CAACGCCAATACATACATAC+CGG 0.590800 4.4:-41390598 None:intergenic
TAACATGCACGGTGGCGGCA+TGG 0.592169 4.4:+41390428 MS.gene055629:CDS
CGTACACGGTTCATTAAACC+CGG 0.599386 4.4:+41390663 MS.gene055629:CDS
GAAACAAACCGGCAGCAACA+TGG 0.616307 4.4:-41390692 None:intergenic
ATGATGGGTCATAAGATGCA+CGG 0.642320 4.4:+41390504 MS.gene055629:CDS
GTACGGACATGACGAAAACA+AGG 0.653972 4.4:-41390623 None:intergenic
AACAATTAACAAGACAAGGG+AGG 0.655863 4.4:-41390885 None:intergenic
TACATACCGGTATCACCGTT+AGG 0.669036 4.4:-41390585 None:intergenic
ATGCACATGACATTCTTCTG+GGG 0.701142 4.4:+41390531 MS.gene055629:CDS
GATCATGGTGGTAACATGCA+CGG 0.714076 4.4:+41390417 MS.gene055629:CDS
CTCACATCCATGGATCATGG+TGG 0.733160 4.4:+41390405 None:intergenic
CATGGTGGTAACATGCACGG+TGG 0.787274 4.4:+41390420 MS.gene055629:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTTAATTCTCTTCAACAAT+TGG + chr4.4:41390562-41390581 MS.gene055629:CDS 25.0%
GAAACCAAAACATGATGATA+AGG + chr4.4:41390851-41390870 MS.gene055629:CDS 30.0%
TGAGAGTAATTCAATGAGTA+CGG - chr4.4:41390643-41390662 None:intergenic 30.0%
! GTTAGCTTATTTGGTTATGT+TGG + chr4.4:41390743-41390762 MS.gene055629:CDS 30.0%
!! TAAGCCTTATCATCATGTTT+TGG - chr4.4:41390858-41390877 None:intergenic 30.0%
!! TTATTGGTTGATGATGATGA+TGG - chr4.4:41390477-41390496 None:intergenic 30.0%
AACCAATAACACCATGATGA+TGG + chr4.4:41390488-41390507 MS.gene055629:CDS 35.0%
ACCAATAACACCATGATGAT+GGG + chr4.4:41390489-41390508 MS.gene055629:CDS 35.0%
TGATACCGGTATGTATGTAT+TGG + chr4.4:41390593-41390612 MS.gene055629:CDS 35.0%
TTGAATTACTCTCACGTACA+CGG + chr4.4:41390649-41390668 MS.gene055629:CDS 35.0%
! AGACACTTTTGCATGTTCTT+AGG + chr4.4:41390715-41390734 MS.gene055629:CDS 35.0%
! GACACTTTTGCATGTTCTTA+GGG + chr4.4:41390716-41390735 MS.gene055629:CDS 35.0%
! TTGGCTCTTATGTCTTTCAA+TGG + chr4.4:41390762-41390781 MS.gene055629:CDS 35.0%
! TTTTGCATGTTCTTAGGGTT+GGG + chr4.4:41390721-41390740 MS.gene055629:CDS 35.0%
!! CAAAAGTGTCTGAAACAAAC+CGG - chr4.4:41390706-41390725 None:intergenic 35.0%
!! TTTCAATGGTGGTGTGTTTT+TGG + chr4.4:41390776-41390795 MS.gene055629:CDS 35.0%
!!! GTTGATGATGATGATGGTTT+TGG - chr4.4:41390471-41390490 None:intergenic 35.0%
ATGCACATGACATTCTTCTG+GGG + chr4.4:41390531-41390550 MS.gene055629:CDS 40.0%
CAACGCCAATACATACATAC+CGG - chr4.4:41390601-41390620 None:intergenic 40.0%
CATCTTATGACCCATCATCA+TGG - chr4.4:41390502-41390521 None:intergenic 40.0%
GATGCACATGACATTCTTCT+GGG + chr4.4:41390530-41390549 MS.gene055629:CDS 40.0%
GCTCTTATGTCTTTCAATGG+TGG + chr4.4:41390765-41390784 MS.gene055629:CDS 40.0%
TGATGCACATGACATTCTTC+TGG + chr4.4:41390529-41390548 MS.gene055629:CDS 40.0%
! CTCTTCAACAATTGGCCTAA+CGG + chr4.4:41390570-41390589 MS.gene055629:CDS 40.0%
! CTTTTGCATGTTCTTAGGGT+TGG + chr4.4:41390720-41390739 MS.gene055629:CDS 40.0%
! TCTTGTGTAGCAGTGCTATT+AGG + chr4.4:41390829-41390848 MS.gene055629:CDS 40.0%
!! ACCCATCATCATGGTGTTAT+TGG - chr4.4:41390493-41390512 None:intergenic 40.0%
!! ATGATGGGTCATAAGATGCA+CGG + chr4.4:41390504-41390523 MS.gene055629:CDS 40.0%
!! TAGGGTTGGGTTAGCTTATT+TGG + chr4.4:41390734-41390753 MS.gene055629:CDS 40.0%
!!! GATGATGATGATGGTTTTGG+TGG - chr4.4:41390468-41390487 None:intergenic 40.0%
CGTACACGGTTCATTAAACC+CGG + chr4.4:41390663-41390682 MS.gene055629:CDS 45.0%
GATCATGGTGGTAACATGCA+CGG + chr4.4:41390417-41390436 MS.gene055629:CDS 45.0%
GTACGGACATGACGAAAACA+AGG - chr4.4:41390626-41390645 None:intergenic 45.0%
TACATACCGGTATCACCGTT+AGG - chr4.4:41390588-41390607 None:intergenic 45.0%
! AATTGGCCTAACGGTGATAC+CGG + chr4.4:41390579-41390598 MS.gene055629:CDS 45.0%
!!! GTGTTTTTGGTTGCTGTGCT+TGG + chr4.4:41390789-41390808 MS.gene055629:CDS 45.0%
GAAACAAACCGGCAGCAACA+TGG - chr4.4:41390695-41390714 None:intergenic 50.0%
CATGGTGGTAACATGCACGG+TGG + chr4.4:41390420-41390439 MS.gene055629:CDS 55.0%
GCAGCAACATGGTTCGAACC+GGG - chr4.4:41390684-41390703 None:intergenic 55.0%
GCTGTGCTTGGTCATGCTCT+TGG + chr4.4:41390801-41390820 MS.gene055629:CDS 55.0%
GGCAGCAACATGGTTCGAAC+CGG - chr4.4:41390685-41390704 None:intergenic 55.0%
GGTTCGAACCATGTTGCTGC+CGG + chr4.4:41390684-41390703 MS.gene055629:CDS 55.0%
GGTGGTAACATGCACGGTGG+CGG + chr4.4:41390423-41390442 MS.gene055629:CDS 60.0%
! TAACATGCACGGTGGCGGCA+TGG + chr4.4:41390428-41390447 MS.gene055629:CDS 60.0%
! ATGCACGGTGGCGGCATGGA+TGG + chr4.4:41390432-41390451 MS.gene055629:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 41390414 41390902 41390414 ID=MS.gene055629
chr4.4 mRNA 41390414 41390902 41390414 ID=MS.gene055629.t1;Parent=MS.gene055629
chr4.4 exon 41390414 41390902 41390414 ID=MS.gene055629.t1.exon1;Parent=MS.gene055629.t1
chr4.4 CDS 41390414 41390902 41390414 ID=cds.MS.gene055629.t1;Parent=MS.gene055629.t1
Gene Sequence

>MS.gene055629

ATGGATCATGGTGGTAACATGCACGGTGGCGGCATGGATGGAATGAACATGCCACCAAAACCATCATCATCATCAACCAATAACACCATGATGATGGGTCATAAGATGCACGGTATGATGCACATGACATTCTTCTGGGGCAAAGATGCTTTAATTCTCTTCAACAATTGGCCTAACGGTGATACCGGTATGTATGTATTGGCGTTGATCCTTGTTTTCGTCATGTCCGTACTCATTGAATTACTCTCACGTACACGGTTCATTAAACCCGGTTCGAACCATGTTGCTGCCGGTTTGTTTCAGACACTTTTGCATGTTCTTAGGGTTGGGTTAGCTTATTTGGTTATGTTGGCTCTTATGTCTTTCAATGGTGGTGTGTTTTTGGTTGCTGTGCTTGGTCATGCTCTTGGATTCTTCTTGTGTAGCAGTGCTATTAGGAAACCAAAACATGATGATAAGGCTTATGATCTTCCTCCCTTGTCTTGTTAA

Protein sequence

>MS.gene055629.t1

MDHGGNMHGGGMDGMNMPPKPSSSSTNNTMMMGHKMHGMMHMTFFWGKDALILFNNWPNGDTGMYVLALILVFVMSVLIELLSRTRFIKPGSNHVAAGLFQTLLHVLRVGLAYLVMLALMSFNGGVFLVAVLGHALGFFLCSSAIRKPKHDDKAYDLPPLSC