AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058420.t1 MTR_1g015275 86.721 369 44 3 1 366 1 367 1.35e-168 475
MS.gene058420.t1 MTR_3g104880 44.146 410 140 14 1 366 1 365 4.32e-53 180
MS.gene058420.t1 MTR_8g019660 40.573 419 172 13 1 367 1 394 7.41e-34 129
MS.gene058420.t1 MTR_4g125620 34.160 363 181 15 17 366 15 332 6.08e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058420.t1 AT3G13980 36.788 386 154 12 16 367 16 345 1.54e-37 139
MS.gene058420.t1 AT1G54200 37.221 403 158 14 4 367 13 359 3.45e-34 130
MS.gene058420.t1 AT5G12050 32.500 400 169 14 16 364 7 356 1.06e-17 84.0
MS.gene058420.t1 AT3G42800 32.308 195 109 5 186 364 140 327 1.79e-13 71.2

Find 64 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCTTGTTCTAACCGGTTT+TGG 0.272024 1.4:+6801433 None:intergenic
ATCTGCTTCTTCGTCTTTGT+TGG 0.273644 1.4:+6801151 None:intergenic
CAAACAAAGCACAGAAAATC+AGG 0.283328 1.4:-6801335 MS.gene058420:CDS
CGTCGATGGAACGGTAAATT+TGG 0.311193 1.4:+6801767 None:intergenic
CAGTTCCACTTCAATTTCTT+CGG 0.312323 1.4:-6801545 MS.gene058420:CDS
CAAGCGTGTCGGCATCATTT+AGG 0.345034 1.4:-6801417 MS.gene058420:CDS
TAGGCTTGAAAAGGAGAAAC+AGG 0.345846 1.4:-6801398 MS.gene058420:CDS
GCTTCACTTTCTTCAAGTTA+TGG 0.350552 1.4:+6801263 None:intergenic
TCCGACACAGATTCATTATA+CGG 0.352790 1.4:-6801496 MS.gene058420:CDS
TTGGATCATTTGAGTGTGTT+TGG 0.380923 1.4:-6800815 MS.gene058420:CDS
GGAAGAGGGTAGGGCTAAGG+TGG 0.392720 1.4:-6800993 MS.gene058420:CDS
GCCGACACGCTTGTTCTAAC+CGG 0.412919 1.4:+6801427 None:intergenic
AGAGGGTAGGGCTAAGGTGG+AGG 0.421619 1.4:-6800990 MS.gene058420:CDS
GTTGGTTTGTTTAAAATCGT+TGG 0.424352 1.4:+6801691 None:intergenic
TTGTGGAGTGGAAGAGGGTA+GGG 0.431294 1.4:-6801002 MS.gene058420:CDS
AGTGGAAGAGGGTAGGGCTA+AGG 0.442934 1.4:-6800996 MS.gene058420:CDS
CGTTCCACCTAGGCCAAAAC+CGG 0.444322 1.4:-6801446 MS.gene058420:CDS
AAGGGTTTATGAACCTCTCC+TGG 0.449214 1.4:+6801812 None:intergenic
TGTGAGGAGCTTCCTGTGTA+TGG 0.451538 1.4:-6800779 MS.gene058420:CDS
ATTTCTTCGGATTCTAGCTC+CGG 0.469115 1.4:-6801532 MS.gene058420:CDS
AACGGTAAATTTGGTCTAAG+AGG 0.473989 1.4:+6801776 None:intergenic
GGCATCATTTAGGCTTGAAA+AGG 0.486316 1.4:-6801407 MS.gene058420:CDS
AAGAGGGTGGAAGAGAATGA+AGG 0.489820 1.4:+6801793 None:intergenic
TTTGTGGAGTGGAAGAGGGT+AGG 0.496413 1.4:-6801003 MS.gene058420:CDS
TGAGTGTGTTTGGAGATAGT+AGG 0.500314 1.4:-6800805 MS.gene058420:CDS
TTTCTCCTTCGTCGATGGAA+CGG 0.507353 1.4:+6801758 None:intergenic
AGAAAATCAGGGCGAGGAAG+CGG 0.507438 1.4:-6801323 MS.gene058420:CDS
TCTTCTTCTTGTTATAACAT+TGG 0.508602 1.4:+6800939 None:intergenic
ATCATTTCTACCACAAAATG+AGG 0.508732 1.4:+6801042 None:intergenic
CTTGGTCGTGATCACGGAGG+TGG 0.530166 1.4:+6801581 None:intergenic
GTGAAGCAACCGATTTCGCC+AGG 0.534045 1.4:-6801247 MS.gene058420:CDS
TGGTAGAAATGATTACAAAA+CGG 0.535489 1.4:-6801032 MS.gene058420:CDS
CGTCGTGCTTCGTTCCACCT+AGG 0.539775 1.4:-6801456 MS.gene058420:CDS
CGAAGAAGCAGATGAACACA+TGG 0.541264 1.4:-6801141 MS.gene058420:CDS
AACTGAAGAACAGAGCATCT+TGG 0.545134 1.4:+6801563 None:intergenic
TGGAGATAGTAGGTATTGTG+AGG 0.545793 1.4:-6800795 MS.gene058420:CDS
GAGCATCTTGGTCGTGATCA+CGG 0.556578 1.4:+6801575 None:intergenic
AGAGGGTGGAAGAGAATGAA+GGG 0.557335 1.4:+6801794 None:intergenic
TAATGAATCTGTGTCGGAGG+AGG 0.558404 1.4:+6801501 None:intergenic
AGAATCCGAAGAAATTGAAG+TGG 0.585201 1.4:+6801540 None:intergenic
AAGCACAGAAAATCAGGGCG+AGG 0.586042 1.4:-6801329 MS.gene058420:CDS
TCTTCGGATTCTAGCTCCGG+TGG 0.592579 1.4:-6801529 MS.gene058420:CDS
TCCGTATAATGAATCTGTGT+CGG 0.592790 1.4:+6801495 None:intergenic
ACGGTAAATTTGGTCTAAGA+GGG 0.594369 1.4:+6801777 None:intergenic
GAACACATGGGAGACTCATG+AGG 0.596864 1.4:-6801128 MS.gene058420:CDS
GTCGGAGGAGGAGAATCCAC+CGG 0.606459 1.4:+6801513 None:intergenic
AAACAAAGCACAGAAAATCA+GGG 0.608992 1.4:-6801334 MS.gene058420:CDS
AATAAAGAACAAATCAAGAG+CGG 0.611789 1.4:-6801296 MS.gene058420:CDS
GTAAATTTGGTCTAAGAGGG+TGG 0.617354 1.4:+6801780 None:intergenic
AGGCTTGAAAAGGAGAAACA+GGG 0.627580 1.4:-6801397 MS.gene058420:CDS
GGATGAAGATGATGATGATG+TGG 0.631674 1.4:-6800867 MS.gene058420:CDS
ATTTACCGTTCCATCGACGA+AGG 0.641766 1.4:-6801763 MS.gene058420:CDS
TGAGAAGAGATCAACAAACC+AGG 0.646589 1.4:-6801830 MS.gene058420:CDS
CGAAGAAGCAGATGAACACG+TGG 0.648153 1.4:+6801093 None:intergenic
ACCGGTTAGAACAAGCGTGT+CGG 0.655186 1.4:-6801428 MS.gene058420:CDS
GGCTTGAAAAGGAGAAACAG+GGG 0.655509 1.4:-6801396 MS.gene058420:CDS
AGAGATCAACAAACCAGGAG+AGG 0.655509 1.4:-6801825 MS.gene058420:CDS
ATTTCTACCACAAAATGAGG+CGG 0.655870 1.4:+6801045 None:intergenic
AAGCAACCGATTTCGCCAGG+TGG 0.662604 1.4:-6801244 MS.gene058420:CDS
GTATAATGAATCTGTGTCGG+AGG 0.665395 1.4:+6801498 None:intergenic
AACACATGGGAGACTCATGA+GGG 0.677376 1.4:-6801127 MS.gene058420:CDS
ACACGAGTAGTTCCATACAC+AGG 0.691654 1.4:+6800767 None:intergenic
GAAGAAGCAGATGAACACAT+GGG 0.731435 1.4:-6801140 MS.gene058420:CDS
CATCTTGGTCGTGATCACGG+AGG 0.775277 1.4:+6801578 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATGATTTAGTTTTATTAA+AGG - chr1.4:6801683-6801702 MS.gene058420:CDS 15.0%
!! TATTAATCAAAAAGAAGAAG+AGG - chr1.4:6801713-6801732 MS.gene058420:CDS 20.0%
! AATAAAGAACAAATCAAGAG+CGG - chr1.4:6801305-6801324 MS.gene058420:CDS 25.0%
! TGGTAGAAATGATTACAAAA+CGG - chr1.4:6801569-6801588 MS.gene058420:CDS 25.0%
!! GTTATTGTTATGTTTTCTGT+CGG + chr1.4:6800938-6800957 None:intergenic 25.0%
!! TCTTCTTCTTGTTATAACAT+TGG + chr1.4:6801665-6801684 None:intergenic 25.0%
!!! GTAAAATTTTGTTGATTCAG+TGG + chr1.4:6800878-6800897 None:intergenic 25.0%
AAACAAAGCACAGAAAATCA+GGG - chr1.4:6801267-6801286 MS.gene058420:CDS 30.0%
ATCATTTCTACCACAAAATG+AGG + chr1.4:6801562-6801581 None:intergenic 30.0%
!! GTTGGTTTGTTTAAAATCGT+TGG + chr1.4:6800913-6800932 None:intergenic 30.0%
!!! AAGTTCTGATCTTTTTGAGT+TGG - chr1.4:6801767-6801786 MS.gene058420:CDS 30.0%
!!! GAAACAGTTTTTTCGGTTTT+TGG + chr1.4:6800970-6800989 None:intergenic 30.0%
AACGGTAAATTTGGTCTAAG+AGG + chr1.4:6800828-6800847 None:intergenic 35.0%
ACGGTAAATTTGGTCTAAGA+GGG + chr1.4:6800827-6800846 None:intergenic 35.0%
AGAATCCGAAGAAATTGAAG+TGG + chr1.4:6801064-6801083 None:intergenic 35.0%
ATTTCTACCACAAAATGAGG+CGG + chr1.4:6801559-6801578 None:intergenic 35.0%
CAAACAAAGCACAGAAAATC+AGG - chr1.4:6801266-6801285 MS.gene058420:CDS 35.0%
CAGTTCCACTTCAATTTCTT+CGG - chr1.4:6801056-6801075 MS.gene058420:CDS 35.0%
TCCGACACAGATTCATTATA+CGG - chr1.4:6801105-6801124 MS.gene058420:CDS 35.0%
TCCGTATAATGAATCTGTGT+CGG + chr1.4:6801109-6801128 None:intergenic 35.0%
TTGGATCATTTGAGTGTGTT+TGG - chr1.4:6801786-6801805 MS.gene058420:CDS 35.0%
! GCTTCACTTTCTTCAAGTTA+TGG + chr1.4:6801341-6801360 None:intergenic 35.0%
! TACAAAACGGTACGTTTTTG+TGG - chr1.4:6801582-6801601 MS.gene058420:CDS 35.0%
!! TTATGTTTTCTGTCGGTTGT+TGG + chr1.4:6800931-6800950 None:intergenic 35.0%
AGGCTTGAAAAGGAGAAACA+GGG - chr1.4:6801204-6801223 MS.gene058420:CDS 40.0%
ATCTGCTTCTTCGTCTTTGT+TGG + chr1.4:6801453-6801472 None:intergenic 40.0%
GAAGAAGCAGATGAACACAT+GGG - chr1.4:6801461-6801480 MS.gene058420:CDS 40.0%
GGCATCATTTAGGCTTGAAA+AGG - chr1.4:6801194-6801213 MS.gene058420:CDS 40.0%
GTAAATTTGGTCTAAGAGGG+TGG + chr1.4:6800824-6800843 None:intergenic 40.0%
GTATAATGAATCTGTGTCGG+AGG + chr1.4:6801106-6801125 None:intergenic 40.0%
TAGGCTTGAAAAGGAGAAAC+AGG - chr1.4:6801203-6801222 MS.gene058420:CDS 40.0%
TGAGAAGAGATCAACAAACC+AGG - chr1.4:6800771-6800790 MS.gene058420:CDS 40.0%
TGAGTGTGTTTGGAGATAGT+AGG - chr1.4:6801796-6801815 MS.gene058420:CDS 40.0%
! AAACTAGTGAGTTTTCCACC+TGG + chr1.4:6801375-6801394 None:intergenic 40.0%
! AACTGAAGAACAGAGCATCT+TGG + chr1.4:6801041-6801060 None:intergenic 40.0%
! GAGGTGGAATTTTCTGTTGT+CGG + chr1.4:6801007-6801026 None:intergenic 40.0%
! GGATGAAGATGATGATGATG+TGG - chr1.4:6801734-6801753 MS.gene058420:CDS 40.0%
! TGGAGATAGTAGGTATTGTG+AGG - chr1.4:6801806-6801825 MS.gene058420:CDS 40.0%
!! ATTTCTTCGGATTCTAGCTC+CGG - chr1.4:6801069-6801088 MS.gene058420:CDS 40.0%
!! TTTTGTTTCTCCTTCGTCGA+TGG + chr1.4:6800851-6800870 None:intergenic 40.0%
!!! CGCGACAGAAACAGTTTTTT+CGG + chr1.4:6800977-6800996 None:intergenic 40.0%
AACACATGGGAGACTCATGA+GGG - chr1.4:6801474-6801493 MS.gene058420:CDS 45.0%
AAGAGGGTGGAAGAGAATGA+AGG + chr1.4:6800811-6800830 None:intergenic 45.0%
AAGGGTTTATGAACCTCTCC+TGG + chr1.4:6800792-6800811 None:intergenic 45.0%
ACACGAGTAGTTCCATACAC+AGG + chr1.4:6801837-6801856 None:intergenic 45.0%
AGAGATCAACAAACCAGGAG+AGG - chr1.4:6800776-6800795 MS.gene058420:CDS 45.0%
AGAGGGTGGAAGAGAATGAA+GGG + chr1.4:6800810-6800829 None:intergenic 45.0%
ATTTACCGTTCCATCGACGA+AGG - chr1.4:6800838-6800857 MS.gene058420:CDS 45.0%
CGAAGAAGCAGATGAACACA+TGG - chr1.4:6801460-6801479 MS.gene058420:CDS 45.0%
CGTCGATGGAACGGTAAATT+TGG + chr1.4:6800837-6800856 None:intergenic 45.0%
GGCTTGAAAAGGAGAAACAG+GGG - chr1.4:6801205-6801224 MS.gene058420:CDS 45.0%
TAATGAATCTGTGTCGGAGG+AGG + chr1.4:6801103-6801122 None:intergenic 45.0%
TTTCTCCTTCGTCGATGGAA+CGG + chr1.4:6800846-6800865 None:intergenic 45.0%
! AACGGTACGTTTTTGTGGAG+TGG - chr1.4:6801587-6801606 MS.gene058420:CDS 45.0%
! ACGTTTTTGTGGAGTGGAAG+AGG - chr1.4:6801593-6801612 MS.gene058420:CDS 45.0%
! AGCAAAACCGCCTCATTTTG+TGG - chr1.4:6801549-6801568 MS.gene058420:CDS 45.0%
! AGTTTTCCACCTGGCGAAAT+CGG + chr1.4:6801366-6801385 None:intergenic 45.0%
! CATCTGCTTCTTCGTTTTCG+CGG - chr1.4:6801517-6801536 MS.gene058420:CDS 45.0%
! CGTTTTTGTGGAGTGGAAGA+GGG - chr1.4:6801594-6801613 MS.gene058420:CDS 45.0%
!! ACGCTTGTTCTAACCGGTTT+TGG + chr1.4:6801171-6801190 None:intergenic 45.0%
AAGCACAGAAAATCAGGGCG+AGG - chr1.4:6801272-6801291 MS.gene058420:CDS 50.0%
ACCGGTTAGAACAAGCGTGT+CGG - chr1.4:6801173-6801192 MS.gene058420:CDS 50.0%
AGAAAATCAGGGCGAGGAAG+CGG - chr1.4:6801278-6801297 MS.gene058420:CDS 50.0%
CGAAGAAGCAGATGAACACG+TGG + chr1.4:6801511-6801530 None:intergenic 50.0%
GAACACATGGGAGACTCATG+AGG - chr1.4:6801473-6801492 MS.gene058420:CDS 50.0%
GAGCATCTTGGTCGTGATCA+CGG + chr1.4:6801029-6801048 None:intergenic 50.0%
TGTGAGGAGCTTCCTGTGTA+TGG - chr1.4:6801822-6801841 MS.gene058420:CDS 50.0%
TTGTGGAGTGGAAGAGGGTA+GGG - chr1.4:6801599-6801618 MS.gene058420:CDS 50.0%
TTTGTGGAGTGGAAGAGGGT+AGG - chr1.4:6801598-6801617 MS.gene058420:CDS 50.0%
! AGGGGAATCACGTTTTCGAC+AGG - chr1.4:6801223-6801242 MS.gene058420:CDS 50.0%
! CAAGCGTGTCGGCATCATTT+AGG - chr1.4:6801184-6801203 MS.gene058420:CDS 50.0%
!! GTTCTAACCGGTTTTGGCCT+AGG + chr1.4:6801165-6801184 None:intergenic 50.0%
AGTGGAAGAGGGTAGGGCTA+AGG - chr1.4:6801605-6801624 MS.gene058420:CDS 55.0%
CATCTTGGTCGTGATCACGG+AGG + chr1.4:6801026-6801045 None:intergenic 55.0%
CGTTCCACCTAGGCCAAAAC+CGG - chr1.4:6801155-6801174 MS.gene058420:CDS 55.0%
GCCGACACGCTTGTTCTAAC+CGG + chr1.4:6801177-6801196 None:intergenic 55.0%
! AAGCAACCGATTTCGCCAGG+TGG - chr1.4:6801357-6801376 MS.gene058420:CDS 55.0%
! GTGAAGCAACCGATTTCGCC+AGG - chr1.4:6801354-6801373 MS.gene058420:CDS 55.0%
!! CTAACCGGTTTTGGCCTAGG+TGG + chr1.4:6801162-6801181 None:intergenic 55.0%
!! TCTTCGGATTCTAGCTCCGG+TGG - chr1.4:6801072-6801091 MS.gene058420:CDS 55.0%
AGAGGGTAGGGCTAAGGTGG+AGG - chr1.4:6801611-6801630 MS.gene058420:CDS 60.0%
CGTCGTGCTTCGTTCCACCT+AGG - chr1.4:6801145-6801164 MS.gene058420:CDS 60.0%
CTTGGTCGTGATCACGGAGG+TGG + chr1.4:6801023-6801042 None:intergenic 60.0%
GGAAGAGGGTAGGGCTAAGG+TGG - chr1.4:6801608-6801627 MS.gene058420:CDS 60.0%
GTCGGAGGAGGAGAATCCAC+CGG + chr1.4:6801091-6801110 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 6800760 6801863 6800760 ID=MS.gene058420
chr1.4 mRNA 6800760 6801863 6800760 ID=MS.gene058420.t1;Parent=MS.gene058420
chr1.4 exon 6800760 6801863 6800760 ID=MS.gene058420.t1.exon1;Parent=MS.gene058420.t1
chr1.4 CDS 6800760 6801863 6800760 ID=cds.MS.gene058420.t1;Parent=MS.gene058420.t1
Gene Sequence

>MS.gene058420

ATGTACAAGTATGAGAAGAGATCAACAAACCAGGAGAGGTTCATAAACCCTTCATTCTCTTCCACCCTCTTAGACCAAATTTACCGTTCCATCGACGAAGGAGAAACAAAATTAACCACTGAATCAACAAAATTTTACAGAGAGCAAAAACCAACGATTTTAAACAAACCAACAACCGACAGAAAACATAACAATAACAACAACAAACCAAAAACCGAAAAAACTGTTTCTGTCGCGAAAGAAACCGACAACAGAAAATTCCACCTCCGTGATCACGACCAAGATGCTCTGTTCTTCAGTTCCACTTCAATTTCTTCGGATTCTAGCTCCGGTGGATTCTCCTCCTCCGACACAGATTCATTATACGGAACGAAGTCACATGCTTCGTCGTGCTTCGTTCCACCTAGGCCAAAACCGGTTAGAACAAGCGTGTCGGCATCATTTAGGCTTGAAAAGGAGAAACAGGGGAATCACGTTTTCGACAGGTTTTGTCGAAGTTCTCAAAACAAACAAAGCACAGAAAATCAGGGCGAGGAAGCGGTTTTAATAAAGAACAAATCAAGAGCGGTTAAGATTTACCATAACTTGAAGAAAGTGAAGCAACCGATTTCGCCAGGTGGAAAACTCACTAGTTTTCTCAACTCTCTATTTGCTAATAGTAACAATGAGAAGAAAACGAAGACAGAGAATCCAACAAAGACGAAGAAGCAGATGAACACATGGGAGACTCATGAGGGAAAAGTAGCTTCCACGTGTTCATCTGCTTCTTCGTTTTCGCGGTCTTGTTTGAGCAAAACCGCCTCATTTTGTGGTAGAAATGATTACAAAACGGTACGTTTTTGTGGAGTGGAAGAGGGTAGGGCTAAGGTGGAGGAAGCTACTAGAAAGTTTTTGAATGAGTACCAATGTTATAACAAGAAGAAGAATGATTTAGTTTTATTAAAGGATTTGTGTATTAATCAAAAAGAAGAAGAGGATGAAGATGATGATGATGTGGCAAGTTGTGCAAGTTCTGATCTTTTTGAGTTGGATCATTTGAGTGTGTTTGGAGATAGTAGGTATTGTGAGGAGCTTCCTGTGTATGGAACTACTCGTGTTAATTAG

Protein sequence

>MS.gene058420.t1

MYKYEKRSTNQERFINPSFSSTLLDQIYRSIDEGETKLTTESTKFYREQKPTILNKPTTDRKHNNNNNKPKTEKTVSVAKETDNRKFHLRDHDQDALFFSSTSISSDSSSGGFSSSDTDSLYGTKSHASSCFVPPRPKPVRTSVSASFRLEKEKQGNHVFDRFCRSSQNKQSTENQGEEAVLIKNKSRAVKIYHNLKKVKQPISPGGKLTSFLNSLFANSNNEKKTKTENPTKTKKQMNTWETHEGKVASTCSSASSFSRSCLSKTASFCGRNDYKTVRFCGVEEGRAKVEEATRKFLNEYQCYNKKKNDLVLLKDLCINQKEEEDEDDDDVASCASSDLFELDHLSVFGDSRYCEELPVYGTTRVN