AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38582


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38582.t1 MTR_3g099570 82.738 168 24 1 1 163 17 184 1.78e-86 251
MS.gene38582.t1 MTR_3g099580 68.421 171 45 3 1 163 17 186 8.39e-58 179
MS.gene38582.t1 MTR_3g099680 58.049 205 39 5 1 159 17 220 1.60e-56 177
MS.gene38582.t1 MTR_3g099980 58.621 203 39 5 1 159 17 218 1.77e-56 176
MS.gene38582.t1 MTR_3g099540 59.091 110 40 1 1 105 18 127 3.23e-38 127
MS.gene38582.t1 MTR_8g007035 50.467 107 45 3 1 99 14 120 1.53e-26 99.8
MS.gene38582.t1 MTR_8g007020 40.964 166 89 4 1 157 14 179 2.04e-26 99.0
MS.gene38582.t1 MTR_1g090190 33.125 160 102 1 7 161 20 179 4.86e-26 97.8
MS.gene38582.t1 MTR_4g114870 41.379 116 58 2 8 113 27 142 1.45e-21 89.7
MS.gene38582.t1 MTR_4g114870 42.574 101 48 2 9 99 190 290 3.45e-19 83.2
MS.gene38582.t1 MTR_2g088990 39.706 136 68 4 6 127 21 156 1.00e-20 85.1
MS.gene38582.t1 MTR_5g006040 36.667 120 68 2 7 118 23 142 6.76e-19 79.3
MS.gene38582.t1 MTR_6g083240 34.177 158 89 4 5 150 22 176 2.52e-18 77.8
MS.gene38582.t1 MTR_8g099220 39.806 103 53 2 7 100 26 128 3.31e-18 76.3
MS.gene38582.t1 MTR_6g080660 38.000 100 57 1 9 103 27 126 9.74e-17 74.3
MS.gene38582.t1 MTR_1g090420 31.618 136 80 4 7 133 25 156 9.96e-17 74.3
MS.gene38582.t1 MTR_7g086140 40.594 101 53 2 6 99 22 122 1.77e-16 72.4
MS.gene38582.t1 MTR_7g086190 40.594 101 53 2 6 99 22 122 1.77e-16 72.4
MS.gene38582.t1 MTR_2g043730 39.216 102 53 3 5 100 27 125 2.90e-16 71.2
MS.gene38582.t1 MTR_2g025580 33.333 117 71 2 1 110 18 134 1.15e-15 70.9
MS.gene38582.t1 MTR_7g086160 39.000 100 54 2 7 99 24 123 4.65e-15 68.9
MS.gene38582.t1 MTR_1g105130 37.624 101 56 2 6 99 21 121 4.71e-15 68.6
MS.gene38582.t1 MTR_1g105120 37.624 101 56 2 6 99 21 121 4.71e-15 68.6
MS.gene38582.t1 MTR_6g023760 37.255 102 58 2 4 99 20 121 1.03e-14 68.2
MS.gene38582.t1 MTR_4g007250 37.500 104 58 2 4 101 23 125 1.47e-14 67.8
MS.gene38582.t1 MTR_0334s0010 37.864 103 56 2 5 99 23 125 2.48e-14 67.8
MS.gene38582.t1 MTR_1g104800 35.644 101 57 3 7 99 22 122 6.52e-14 66.2
MS.gene38582.t1 MTR_7g086220 36.264 91 52 1 6 90 21 111 1.08e-13 65.1
MS.gene38582.t1 MTR_8g463180 33.333 120 71 3 5 116 19 137 1.98e-13 66.6
MS.gene38582.t1 MTR_4g067200 37.815 119 62 4 1 107 19 137 1.99e-13 64.7
MS.gene38582.t1 MTR_4g066110 37.815 119 62 4 1 107 19 137 1.99e-13 64.7
MS.gene38582.t1 MTR_2g101300 34.677 124 67 4 7 117 24 146 3.21e-13 64.7
MS.gene38582.t1 MTR_7g086100 33.654 104 63 1 6 103 22 125 8.11e-13 64.3
MS.gene38582.t1 MTR_2g043790 38.384 99 52 3 5 97 2 97 1.02e-12 61.2
MS.gene38582.t1 MTR_7g086090 35.652 115 68 1 6 114 22 136 6.68e-12 62.0
MS.gene38582.t1 MTR_4g077787 27.407 135 85 4 12 134 34 167 3.82e-11 58.9
MS.gene38582.t1 MTR_4g130800 38.272 81 44 1 25 99 49 129 4.22e-11 60.1
MS.gene38582.t1 MTR_7g086280 31.731 104 65 1 6 103 22 125 4.24e-11 58.5
MS.gene38582.t1 MTR_6g022170 34.314 102 58 2 7 99 31 132 4.31e-11 58.5
MS.gene38582.t1 MTR_7g086230 31.731 104 65 1 6 103 22 125 4.60e-11 58.5
MS.gene38582.t1 MTR_4g130800 38.272 81 44 1 25 99 48 128 5.87e-11 59.3
MS.gene38582.t1 MTR_3g089035 37.113 97 52 3 7 97 30 123 6.03e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38582.t1 AT1G72230 46.207 145 62 3 18 152 31 169 7.17e-33 115
MS.gene38582.t1 AT1G22480 54.255 94 38 1 16 104 25 118 5.62e-25 95.1
MS.gene38582.t1 AT2G32300 41.379 116 62 1 5 114 21 136 7.04e-23 91.7
MS.gene38582.t1 AT2G32300 41.379 116 62 1 5 114 51 166 1.54e-22 91.3
MS.gene38582.t1 AT2G31050 33.962 159 83 3 7 143 26 184 7.72e-22 87.4
MS.gene38582.t1 AT2G26720 39.823 113 61 2 7 112 26 138 1.83e-21 86.7
MS.gene38582.t1 AT3G60270 44.000 100 51 1 5 99 21 120 2.18e-21 85.9
MS.gene38582.t1 AT5G07475 37.762 143 73 4 7 140 27 162 1.60e-20 84.0
MS.gene38582.t1 AT3G27200 38.000 100 56 2 6 99 22 121 3.95e-18 77.4
MS.gene38582.t1 AT2G44790 46.316 95 44 2 18 105 36 130 5.63e-18 77.4
MS.gene38582.t1 AT3G17675 35.514 107 63 1 1 101 23 129 2.61e-17 73.9
MS.gene38582.t1 AT5G20230 35.976 164 81 6 10 149 25 188 1.47e-14 68.2
MS.gene38582.t1 AT5G26330 36.634 101 56 2 7 99 21 121 8.27e-14 66.2
MS.gene38582.t1 AT3G60280 37.607 117 68 1 6 117 20 136 1.54e-11 60.5
MS.gene38582.t1 AT5G53870 28.571 147 92 3 12 145 32 178 3.47e-11 60.5
MS.gene38582.t1 AT5G53870 28.571 147 92 3 12 145 32 178 3.99e-11 60.5

Find 42 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTATGGCATTGCCGACTA+TGG 0.299229 3.3:-82002092 None:intergenic
ATTCCTAGACATTGCACATT+TGG 0.319782 3.3:-82001718 MS.gene38582:CDS
CACATTGTTGGAGATGGTTT+AGG 0.350314 3.3:-82002061 MS.gene38582:CDS
TGGCATGAAACTCTCTGTTA+AGG 0.390798 3.3:-82001698 MS.gene38582:CDS
GATCTCTATCCGTCATATAT+TGG 0.416236 3.3:-82001506 MS.gene38582:CDS
GATGGTTTAGGTTGGACAAT+TGG 0.425937 3.3:-82002049 MS.gene38582:CDS
CTAGCCCATGTAGTGTAATC+AGG 0.453147 3.3:+82002025 None:intergenic
TATATGACGGATAGAGATCC+AGG 0.466675 3.3:+82001510 None:intergenic
TTCCAGCTGTGCATAATGTA+TGG 0.484126 3.3:+82001818 None:intergenic
ATGGCAACAACCCACATTGT+TGG 0.499388 3.3:-82002073 MS.gene38582:CDS
ATGCCAAATGTGCAATGTCT+AGG 0.500016 3.3:+82001715 None:intergenic
GACCATACATTATGCACAGC+TGG 0.503333 3.3:-82001820 MS.gene38582:CDS
TTGGTCCTGATTACACTACA+TGG 0.504799 3.3:-82002030 MS.gene38582:CDS
ATCGGAAGGTGAACCTTTCG+TGG 0.505016 3.3:+82001633 None:intergenic
GCAGAAGGAGCGGCAGAAGA+AGG 0.505108 3.3:+82001670 None:intergenic
GGTGGTGGTTGTTGCGGCAG+CGG 0.517819 3.3:+82001606 None:intergenic
AACAATTGGTGAGATATTAG+TGG 0.520976 3.3:+82001552 None:intergenic
TGTTGCGGCAGCGGTGCGAT+CGG 0.526820 3.3:+82001615 None:intergenic
GCGGCAGCGGTGCGATCGGA+AGG 0.527471 3.3:+82001619 None:intergenic
ACAACCCACATTGTTGGAGA+TGG 0.532220 3.3:-82002067 MS.gene38582:CDS
GAAGGTGAACCTTTCGTGGA+TGG 0.547718 3.3:+82001637 None:intergenic
TTTGCAGTTGGTGACAGTCT+CGG 0.554211 3.3:-82001992 MS.gene38582:intron
TGTGGTAGGAGTAGTGGTGG+TGG 0.559960 3.3:+82001591 None:intergenic
ATGTGGGTTGTTGCCATAGT+CGG 0.565131 3.3:+82002079 None:intergenic
GAAGATGCACTTTGTGTGGT+AGG 0.585213 3.3:+82001577 None:intergenic
TTGTTGGAGATGGTTTAGGT+TGG 0.586690 3.3:-82002057 MS.gene38582:CDS
AGTGACAAAACCTTTGCAGT+TGG 0.590211 3.3:-82002004 MS.gene38582:CDS
GAACCTTTCGTGGATGGTGA+TGG 0.595492 3.3:+82001643 None:intergenic
AGTAGTGGTGGTGGTTGTTG+CGG 0.603984 3.3:+82001600 None:intergenic
GATGGTGATGGTGTTGCAGA+AGG 0.618024 3.3:+82001655 None:intergenic
ACTTTGTGTGGTAGGAGTAG+TGG 0.618884 3.3:+82001585 None:intergenic
GAGACTGTCACCAACTGCAA+AGG 0.629029 3.3:+82001994 None:intergenic
TGGTCCTGATTACACTACAT+GGG 0.630389 3.3:-82002029 MS.gene38582:CDS
TAAACCATCTCCAACAATGT+GGG 0.636144 3.3:+82002063 None:intergenic
ACACCATCACCATCCACGAA+AGG 0.636152 3.3:-82001646 MS.gene38582:CDS
GGCTGAAGATGCACTTTGTG+TGG 0.645311 3.3:+82001573 None:intergenic
TATCTCACACCAATATATGA+CGG 0.646155 3.3:+82001497 None:intergenic
TTGTGTGGTAGGAGTAGTGG+TGG 0.649594 3.3:+82001588 None:intergenic
TCAATTAATACAGACAGCAG+TGG 0.671855 3.3:-82001793 MS.gene38582:CDS
CTAAACCATCTCCAACAATG+TGG 0.675206 3.3:+82002062 None:intergenic
TGATGGTGTTGCAGAAGGAG+CGG 0.712627 3.3:+82001660 None:intergenic
CTATGTAGCTGGGCACACAG+TGG 0.727318 3.3:-82001860 MS.gene38582:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACAATTGGTGAGATATTAG+TGG + chr3.3:82002040-82002059 None:intergenic 30.0%
GTGAAAAAGAAAGCAACAAT+TGG + chr3.3:82002054-82002073 None:intergenic 30.0%
!!! AATGTGTTCCTGCTTTTTTT+AGG + chr3.3:82001837-82001856 None:intergenic 30.0%
!!! ATGTGTTCCTGCTTTTTTTA+GGG + chr3.3:82001836-82001855 None:intergenic 30.0%
ATTCCTAGACATTGCACATT+TGG - chr3.3:82001871-82001890 MS.gene38582:intron 35.0%
GATCTCTATCCGTCATATAT+TGG - chr3.3:82002083-82002102 MS.gene38582:CDS 35.0%
TAAACCATCTCCAACAATGT+GGG + chr3.3:82001529-82001548 None:intergenic 35.0%
TCAATTAATACAGACAGCAG+TGG - chr3.3:82001796-82001815 MS.gene38582:CDS 35.0%
!!! TGTGTTCCTGCTTTTTTTAG+GGG + chr3.3:82001835-82001854 None:intergenic 35.0%
!!! TTTTTTTAGGGGAATGGTTG+TGG + chr3.3:82001824-82001843 None:intergenic 35.0%
ACAGTGTTCAACTATGTAGC+TGG - chr3.3:82001718-82001737 MS.gene38582:CDS 40.0%
ACCATTCCCCTAAAAAAAGC+AGG - chr3.3:82001826-82001845 MS.gene38582:CDS 40.0%
AGTGACAAAACCTTTGCAGT+TGG - chr3.3:82001585-82001604 MS.gene38582:CDS 40.0%
ATGCCAAATGTGCAATGTCT+AGG + chr3.3:82001877-82001896 None:intergenic 40.0%
CACATTGTTGGAGATGGTTT+AGG - chr3.3:82001528-82001547 MS.gene38582:CDS 40.0%
CAGTGTTCAACTATGTAGCT+GGG - chr3.3:82001719-82001738 MS.gene38582:CDS 40.0%
CTAAACCATCTCCAACAATG+TGG + chr3.3:82001530-82001549 None:intergenic 40.0%
TGGCATGAAACTCTCTGTTA+AGG - chr3.3:82001891-82001910 MS.gene38582:intron 40.0%
TGGTCCTGATTACACTACAT+GGG - chr3.3:82001560-82001579 MS.gene38582:CDS 40.0%
TTCCAGCTGTGCATAATGTA+TGG + chr3.3:82001774-82001793 None:intergenic 40.0%
TTGGTCCTGATTACACTACA+TGG - chr3.3:82001559-82001578 MS.gene38582:CDS 40.0%
TTGTTGGAGATGGTTTAGGT+TGG - chr3.3:82001532-82001551 MS.gene38582:CDS 40.0%
!! CTTTCTTTTTCACTGCTTCC+TGG - chr3.3:82002061-82002080 MS.gene38582:CDS 40.0%
!! GATGGTTTAGGTTGGACAAT+TGG - chr3.3:82001540-82001559 MS.gene38582:CDS 40.0%
!! TATATGACGGATAGAGATCC+AGG + chr3.3:82002082-82002101 None:intergenic 40.0%
!!! TCCTGCTTTTTTTAGGGGAA+TGG + chr3.3:82001830-82001849 None:intergenic 40.0%
ACAACCCACATTGTTGGAGA+TGG - chr3.3:82001522-82001541 MS.gene38582:CDS 45.0%
ACTTTGTGTGGTAGGAGTAG+TGG + chr3.3:82002007-82002026 None:intergenic 45.0%
ATGGCAACAACCCACATTGT+TGG - chr3.3:82001516-82001535 MS.gene38582:CDS 45.0%
CTAGCCCATGTAGTGTAATC+AGG + chr3.3:82001567-82001586 None:intergenic 45.0%
GAAGATGCACTTTGTGTGGT+AGG + chr3.3:82002015-82002034 None:intergenic 45.0%
GACCATACATTATGCACAGC+TGG - chr3.3:82001769-82001788 MS.gene38582:CDS 45.0%
! ATGTGGGTTGTTGCCATAGT+CGG + chr3.3:82001513-82001532 None:intergenic 45.0%
!! TTTGCAGTTGGTGACAGTCT+CGG - chr3.3:82001597-82001616 MS.gene38582:CDS 45.0%
ACACCATCACCATCCACGAA+AGG - chr3.3:82001943-82001962 MS.gene38582:intron 50.0%
ATCGGAAGGTGAACCTTTCG+TGG + chr3.3:82001959-82001978 None:intergenic 50.0%
GAACCTTTCGTGGATGGTGA+TGG + chr3.3:82001949-82001968 None:intergenic 50.0%
GAAGGTGAACCTTTCGTGGA+TGG + chr3.3:82001955-82001974 None:intergenic 50.0%
GAGACTGTCACCAACTGCAA+AGG + chr3.3:82001598-82001617 None:intergenic 50.0%
GGCTGAAGATGCACTTTGTG+TGG + chr3.3:82002019-82002038 None:intergenic 50.0%
TTGTGTGGTAGGAGTAGTGG+TGG + chr3.3:82002004-82002023 None:intergenic 50.0%
!! AGTAGTGGTGGTGGTTGTTG+CGG + chr3.3:82001992-82002011 None:intergenic 50.0%
!! GATGGTGATGGTGTTGCAGA+AGG + chr3.3:82001937-82001956 None:intergenic 50.0%
!! TGATGGTGTTGCAGAAGGAG+CGG + chr3.3:82001932-82001951 None:intergenic 50.0%
CTATGTAGCTGGGCACACAG+TGG - chr3.3:82001729-82001748 MS.gene38582:CDS 55.0%
TGTGGTAGGAGTAGTGGTGG+TGG + chr3.3:82002001-82002020 None:intergenic 55.0%
GCAGAAGGAGCGGCAGAAGA+AGG + chr3.3:82001922-82001941 None:intergenic 60.0%
TGTTGCGGCAGCGGTGCGAT+CGG + chr3.3:82001977-82001996 None:intergenic 65.0%
! GGTGGTGGTTGTTGCGGCAG+CGG + chr3.3:82001986-82002005 None:intergenic 65.0%
GCGGCAGCGGTGCGATCGGA+AGG + chr3.3:82001973-82001992 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 82001501 82002110 82001501 ID=MS.gene38582
chr3.3 mRNA 82001501 82002110 82001501 ID=MS.gene38582.t1;Parent=MS.gene38582
chr3.3 exon 82001993 82002110 82001993 ID=MS.gene38582.t1.exon1;Parent=MS.gene38582.t1
chr3.3 CDS 82001993 82002110 82001993 ID=cds.MS.gene38582.t1;Parent=MS.gene38582.t1
chr3.3 exon 82001501 82001874 82001501 ID=MS.gene38582.t1.exon2;Parent=MS.gene38582.t1
chr3.3 CDS 82001501 82001874 82001501 ID=cds.MS.gene38582.t1;Parent=MS.gene38582.t1
Gene Sequence

>MS.gene38582

ATGGCATTGCCGACTATGGCAACAACCCACATTGTTGGAGATGGTTTAGGTTGGACAATTGGTCCTGATTACACTACATGGGCTAGTGACAAAACCTTTGCAGTTGGTGACAGTCTCGGTAAGTATAATAATTTAACGAACTTTTATCAATTTCATTTTTTTTTAGTTTTAAATTGCAATCACAATTTACGAAACTAATGTATGTAACTTTACTATGACAGTGTTCAACTATGTAGCTGGGCACACAGTGGATGAAGTTAAAGAAAGTGACCATACATTATGCACAGCTGGAAATTCAATTAATACAGACAGCAGTGGTGCCACAACCATTCCCCTAAAAAAAGCAGGAACACATTACTTCATATGTGCTATTCCTAGACATTGCACATTTGGCATGAAACTCTCTGTTAAGGTTAATCCTTCTTCTGCCGCTCCTTCTGCAACACCATCACCATCCACGAAAGGTTCACCTTCCGATCGCACCGCTGCCGCAACAACCACCACCACTACTCCTACCACACAAAGTGCATCTTCAGCCACTAATATCTCACCAATTGTTGCTTTCTTTTTCACTGCTTCCTGGATCTCTATCCGTCATATATTGGTGTGA

Protein sequence

>MS.gene38582.t1

MALPTMATTHIVGDGLGWTIGPDYTTWASDKTFAVGDSLAGHTVDEVKESDHTLCTAGNSINTDSSGATTIPLKKAGTHYFICAIPRHCTFGMKLSVKVNPSSAAPSATPSPSTKGSPSDRTAAATTTTTTPTTQSASSATNISPIVAFFFTASWISIRHILV