AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39549

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39549.t1 MTR_4g088005 94.949 99 5 0 1 99 21 119 1.66e-68 201
MS.gene39549.t1 MTR_2g015040 68.468 111 27 3 1 103 21 131 3.46e-47 149
MS.gene39549.t1 MTR_8g006840 46.617 133 42 5 1 104 64 196 1.37e-32 113
MS.gene39549.t1 MTR_4g100380 69.118 68 21 0 27 94 135 202 1.19e-31 112
MS.gene39549.t1 MTR_1g070070 60.976 82 32 0 17 98 1 82 7.69e-31 107
MS.gene39549.t1 MTR_7g096700 65.385 78 25 1 26 101 9 86 9.39e-31 107
MS.gene39549.t1 MTR_7g081805 63.636 88 26 3 8 95 38 119 1.43e-30 108
MS.gene39549.t1 MTR_7g081795 63.514 74 27 0 35 108 58 131 4.06e-30 107
MS.gene39549.t1 MTR_7g081815 65.789 76 23 1 35 110 59 131 5.00e-30 107
MS.gene39549.t1 MTR_2g015050 65.753 73 25 0 22 94 119 191 7.85e-30 108
MS.gene39549.t1 MTR_8g006815 64.384 73 22 1 37 105 96 168 1.31e-29 105
MS.gene39549.t1 MTR_4g100450 66.667 69 23 0 26 94 143 211 1.41e-29 108
MS.gene39549.t1 MTR_7g021010 62.069 87 30 2 10 94 22 107 1.93e-29 104
MS.gene39549.t1 MTR_7g020980 60.920 87 31 2 10 94 22 107 3.70e-29 103
MS.gene39549.t1 MTR_1g048610 74.194 62 16 0 35 96 87 148 5.26e-29 105
MS.gene39549.t1 MTR_8g006825 72.131 61 17 0 35 95 94 154 1.35e-28 103
MS.gene39549.t1 MTR_4g100420 60.811 74 29 0 26 99 146 219 1.73e-28 105
MS.gene39549.t1 MTR_7g096750 64.789 71 25 0 26 96 9 79 3.52e-28 100
MS.gene39549.t1 MTR_4g054360 69.118 68 18 1 32 96 116 183 6.55e-28 103
MS.gene39549.t1 MTR_4g054740 53.846 91 35 2 13 96 66 156 2.18e-27 101
MS.gene39549.t1 MTR_7g021010 70.769 65 18 1 30 94 5 68 2.74e-27 98.2
MS.gene39549.t1 MTR_1g074230 69.355 62 19 0 37 98 19 80 4.11e-27 97.8
MS.gene39549.t1 MTR_1g074310 55.294 85 36 1 20 102 2 86 7.16e-27 97.1
MS.gene39549.t1 MTR_1g090290 57.317 82 30 1 13 94 94 170 8.81e-27 98.6
MS.gene39549.t1 MTR_1g070035 69.355 62 19 0 32 93 72 133 1.36e-26 98.6
MS.gene39549.t1 MTR_4g054860 68.254 63 20 0 34 96 128 190 3.74e-26 98.6
MS.gene39549.t1 MTR_1g074370 50.442 113 49 2 1 113 38 143 1.18e-25 95.9
MS.gene39549.t1 MTR_5g009620 58.025 81 30 2 31 108 106 185 1.88e-25 97.4
MS.gene39549.t1 MTR_1g070015 52.273 88 35 1 6 93 60 140 2.94e-25 95.1
MS.gene39549.t1 MTR_1g070045 58.571 70 29 0 24 93 53 122 3.88e-25 93.6
MS.gene39549.t1 MTR_1g069985 66.129 62 21 0 32 93 70 131 7.32e-25 94.0
MS.gene39549.t1 MTR_1g070020 62.500 64 24 0 30 93 63 126 4.72e-24 92.0
MS.gene39549.t1 MTR_1g070000 64.062 64 23 0 30 93 77 140 5.00e-24 92.0
MS.gene39549.t1 MTR_2g435590 68.182 66 20 1 30 95 89 153 8.99e-24 92.0
MS.gene39549.t1 MTR_2g438180 65.574 61 21 0 37 97 14 74 9.11e-24 89.0
MS.gene39549.t1 MTR_7g096810 62.821 78 24 1 37 109 13 90 2.47e-23 87.8
MS.gene39549.t1 MTR_2g078680 45.536 112 49 3 11 112 6 115 4.78e-23 89.0
MS.gene39549.t1 MTR_2g105380 48.980 98 47 2 22 117 102 198 4.79e-23 92.0
MS.gene39549.t1 MTR_1g074250 65.079 63 21 1 37 98 19 81 9.22e-23 86.3
MS.gene39549.t1 MTR_3g074130 56.627 83 33 2 13 95 128 207 2.20e-22 89.7
MS.gene39549.t1 MTR_8g022820 58.667 75 30 1 22 96 108 181 3.00e-22 89.7
MS.gene39549.t1 MTR_4g086165 66.129 62 20 1 33 94 78 138 3.17e-22 87.0
MS.gene39549.t1 MTR_8g022820 58.667 75 30 1 22 96 109 182 3.18e-22 89.7
MS.gene39549.t1 MTR_8g022820 58.667 75 30 1 22 96 109 182 3.18e-22 89.7
MS.gene39549.t1 MTR_8g092460 53.750 80 36 1 33 112 141 219 3.80e-22 89.0
MS.gene39549.t1 MTR_4g086160 68.966 58 17 1 37 94 96 152 4.94e-22 87.0
MS.gene39549.t1 MTR_4g086190 68.966 58 17 1 37 94 96 152 8.77e-22 86.3
MS.gene39549.t1 MTR_1g087920 58.228 79 29 2 18 96 61 135 1.02e-21 87.4
MS.gene39549.t1 MTR_4g086220 68.966 58 17 1 37 94 96 152 1.08e-21 86.3
MS.gene39549.t1 MTR_8g090350 65.574 61 20 1 35 95 111 170 1.88e-21 86.7
MS.gene39549.t1 MTR_2g014300 64.516 62 21 1 33 94 11 71 4.29e-21 83.2
MS.gene39549.t1 MTR_2g014340 63.492 63 22 1 32 94 14 75 7.39e-21 83.2
MS.gene39549.t1 MTR_1g069945 54.945 91 34 2 5 95 76 159 8.61e-21 84.0
MS.gene39549.t1 MTR_5g085070 56.522 69 29 1 26 94 40 107 9.51e-21 84.7
MS.gene39549.t1 MTR_8g099215 57.534 73 30 1 35 107 105 176 1.24e-20 84.7
MS.gene39549.t1 MTR_5g062700 67.213 61 19 1 35 95 171 230 1.42e-20 85.1
MS.gene39549.t1 MTR_4g078710 57.534 73 29 2 37 109 28 98 1.53e-20 82.8
MS.gene39549.t1 MTR_1g090170 47.253 91 41 2 11 95 120 209 1.70e-20 84.3
MS.gene39549.t1 MTR_1g040430 69.231 52 16 0 43 94 117 168 1.72e-20 82.4
MS.gene39549.t1 MTR_1g090170 47.253 91 41 2 11 95 207 296 1.91e-20 85.1
MS.gene39549.t1 MTR_3g053690 63.492 63 22 1 32 94 22 83 2.22e-20 82.4
MS.gene39549.t1 MTR_5g085100 63.492 63 22 1 32 94 41 102 2.26e-20 83.6
MS.gene39549.t1 MTR_1g043350 49.462 93 46 1 1 93 47 138 2.77e-20 82.0
MS.gene39549.t1 MTR_1g069960 62.500 64 24 0 37 100 27 90 3.15e-20 80.5
MS.gene39549.t1 MTR_1g069940 54.945 91 34 2 5 95 70 153 3.43e-20 82.4
MS.gene39549.t1 MTR_2g090235 62.295 61 22 1 37 97 218 277 4.75e-20 84.0
MS.gene39549.t1 MTR_7g096830 65.625 64 22 0 33 96 94 157 7.97e-20 81.3
MS.gene39549.t1 MTR_7g092190 62.712 59 21 1 37 95 30 87 8.03e-20 82.4
MS.gene39549.t1 MTR_1g028560 61.667 60 22 1 34 93 112 170 1.56e-19 82.0
MS.gene39549.t1 MTR_3g090760 62.712 59 21 1 35 93 111 168 3.47e-19 81.3
MS.gene39549.t1 MTR_6g088405 52.632 76 33 3 37 111 44 117 3.96e-19 79.3
MS.gene39549.t1 MTR_2g089170 58.065 62 25 1 34 95 62 122 4.28e-19 78.6
MS.gene39549.t1 MTR_8g069360 66.667 57 18 1 37 93 44 99 5.19e-19 81.3
MS.gene39549.t1 MTR_3g102100 41.489 94 51 2 5 95 10 102 5.51e-19 77.8
MS.gene39549.t1 MTR_7g096780 67.213 61 20 0 33 93 81 141 6.89e-19 79.0
MS.gene39549.t1 MTR_5g083330 50.633 79 36 2 23 99 21 98 2.07e-18 78.6
MS.gene39549.t1 MTR_3g110205 54.688 64 28 1 31 94 37 99 2.55e-18 76.3
MS.gene39549.t1 MTR_7g117690 50.704 71 33 2 37 107 28 96 2.78e-18 75.9
MS.gene39549.t1 MTR_5g075570 54.688 64 28 1 31 94 118 180 4.08e-18 77.4
MS.gene39549.t1 MTR_0939s0010 50.617 81 35 2 36 112 64 143 4.50e-18 75.9
MS.gene39549.t1 MTR_7g084370 54.667 75 32 2 37 110 42 115 6.76e-18 77.0
MS.gene39549.t1 MTR_5g083340 60.656 61 23 1 34 94 82 141 6.78e-18 78.2
MS.gene39549.t1 MTR_8g013860 52.381 63 29 1 33 95 43 104 7.35e-18 75.5
MS.gene39549.t1 MTR_8g006410 49.333 75 37 1 37 111 52 125 9.90e-18 75.5
MS.gene39549.t1 MTR_3g098580 55.814 86 37 1 9 94 48 132 1.52e-17 75.9
MS.gene39549.t1 MTR_4g114570 48.780 82 33 2 32 113 2 74 1.57e-17 75.1
MS.gene39549.t1 MTR_4g008860 45.349 86 46 1 33 118 4 88 1.86e-17 74.3
MS.gene39549.t1 MTR_5g032820 70.175 57 16 1 37 93 46 101 1.99e-17 76.6
MS.gene39549.t1 MTR_4g010640 54.286 70 31 1 32 101 2 70 2.14e-17 74.7
MS.gene39549.t1 MTR_7g046260 42.708 96 47 2 23 111 40 134 3.41e-17 74.3
MS.gene39549.t1 MTR_4g035430 46.753 77 40 1 37 113 32 107 3.75e-17 73.9
MS.gene39549.t1 MTR_2g043050 54.839 62 23 2 37 96 19 77 5.51e-17 73.6
MS.gene39549.t1 MTR_8g027465 51.667 60 28 1 37 96 39 97 5.95e-17 72.8
MS.gene39549.t1 MTR_5g008540 43.011 93 48 4 12 104 2 89 6.57e-17 70.5
MS.gene39549.t1 MTR_4g119270 49.333 75 37 1 37 111 53 126 8.76e-17 74.7
MS.gene39549.t1 MTR_2g103700 55.556 63 27 1 32 94 2 63 1.22e-16 71.6
MS.gene39549.t1 MTR_3g106290 51.562 64 30 1 33 96 4 66 1.56e-16 71.6
MS.gene39549.t1 MTR_1g019110 52.459 61 28 1 35 95 42 101 1.75e-16 71.6
MS.gene39549.t1 MTR_8g102800 46.667 75 39 1 37 111 37 110 1.84e-16 72.4
MS.gene39549.t1 MTR_4g100650 44.156 77 42 1 35 111 72 147 2.00e-16 72.8
MS.gene39549.t1 MTR_6g029180 47.674 86 44 1 30 115 26 110 2.17e-16 73.2
MS.gene39549.t1 MTR_2g043030 53.226 62 24 2 37 96 19 77 2.68e-16 71.6
MS.gene39549.t1 MTR_2g101340 44.000 75 41 1 37 111 41 114 3.15e-16 71.2
MS.gene39549.t1 MTR_5g012910 46.667 75 39 1 37 111 28 101 3.57e-16 70.9
MS.gene39549.t1 MTR_1g074280 48.000 75 39 0 37 111 17 91 4.32e-16 68.9
MS.gene39549.t1 MTR_2g019750 54.688 64 28 1 37 100 50 112 4.48e-16 72.4
MS.gene39549.t1 MTR_3g095190 66.154 65 21 1 34 98 167 230 1.19e-15 71.6
MS.gene39549.t1 MTR_8g085960 57.895 57 23 1 37 93 25 80 1.45e-15 70.1
MS.gene39549.t1 MTR_5g016750 40.000 85 49 2 34 118 22 104 2.38e-15 68.6
MS.gene39549.t1 MTR_1g074290 48.000 75 39 0 37 111 17 91 2.71e-15 67.8
MS.gene39549.t1 MTR_2g043040 51.613 62 25 2 37 96 19 77 3.03e-15 69.3
MS.gene39549.t1 MTR_6g033255 74.194 62 15 1 33 94 16 76 3.11e-15 68.9
MS.gene39549.t1 MTR_5g082950 57.895 57 23 1 37 93 66 121 3.19e-15 70.1
MS.gene39549.t1 MTR_7g070220 54.688 64 23 1 36 93 77 140 3.51e-15 70.5
MS.gene39549.t1 MTR_4g111915 52.174 69 28 2 34 98 50 117 3.96e-15 67.0
MS.gene39549.t1 MTR_1g014800 38.776 98 54 4 22 116 17 111 4.02e-15 68.6
MS.gene39549.t1 MTR_5g008470 51.786 56 26 1 39 94 77 131 5.33e-15 67.8
MS.gene39549.t1 MTR_7g085810 46.341 82 43 1 37 118 30 110 7.44e-15 68.6
MS.gene39549.t1 MTR_4g112490 52.174 69 28 2 34 98 50 117 8.32e-15 67.4
MS.gene39549.t1 MTR_2g016730 49.367 79 37 2 17 93 1 78 8.66e-15 68.9
MS.gene39549.t1 MTR_4g102670 41.975 81 45 2 33 113 11 89 1.00e-14 66.6
MS.gene39549.t1 MTR_1g014860 47.619 63 32 1 33 95 18 79 1.24e-14 67.4
MS.gene39549.t1 MTR_6g012970 56.667 60 25 1 34 93 35 93 1.62e-14 68.6
MS.gene39549.t1 MTR_1g014820 47.692 65 29 2 33 95 23 84 1.76e-14 67.4
MS.gene39549.t1 MTR_1g014780 47.692 65 29 2 33 95 34 95 2.43e-14 66.6
MS.gene39549.t1 MTR_1g097190 52.239 67 30 2 37 102 23 88 3.14e-14 64.7
MS.gene39549.t1 MTR_8g032610 48.571 70 35 1 24 93 14 82 3.44e-14 67.0
MS.gene39549.t1 MTR_2g085015 40.789 76 44 1 37 111 47 122 4.22e-14 66.2
MS.gene39549.t1 MTR_1g006660 57.627 59 24 1 37 95 47 104 4.83e-14 66.2
MS.gene39549.t1 MTR_2g043020 50.000 62 26 2 37 96 19 77 6.21e-14 65.1
MS.gene39549.t1 MTR_1g101600 39.175 97 55 3 13 109 19 111 9.50e-14 64.7
MS.gene39549.t1 MTR_3g017420 58.000 50 20 1 45 94 3 51 9.94e-14 61.6
MS.gene39549.t1 MTR_3g105510 39.362 94 49 4 25 116 27 114 1.12e-13 65.1
MS.gene39549.t1 MTR_6g088425 51.429 70 32 2 33 102 45 112 1.96e-13 64.3
MS.gene39549.t1 MTR_7g011630 44.444 90 40 3 4 93 18 97 2.53e-13 65.1
MS.gene39549.t1 MTR_1g074270 60.417 48 18 1 52 98 22 69 2.73e-13 61.6
MS.gene39549.t1 MTR_4g082345 53.333 60 27 1 34 93 45 103 4.01e-13 64.3
MS.gene39549.t1 MTR_5g085125 41.975 81 46 1 33 113 12 91 6.37e-13 62.0
MS.gene39549.t1 MTR_8g023700 57.692 52 19 2 37 86 117 167 6.88e-13 63.9
MS.gene39549.t1 MTR_6g466130 38.824 85 50 2 25 109 54 136 1.27e-12 62.0
MS.gene39549.t1 MTR_6g465510 46.377 69 36 1 25 93 21 88 2.46e-12 61.2
MS.gene39549.t1 MTR_5g008790 44.776 67 34 2 35 99 16 81 2.56e-12 60.1
MS.gene39549.t1 MTR_6g465850 42.466 73 40 2 37 109 63 133 2.61e-12 61.6
MS.gene39549.t1 MTR_5g008550 38.384 99 53 3 12 110 2 92 3.04e-12 60.5
MS.gene39549.t1 MTR_1g023170 49.333 75 33 2 21 95 28 97 4.71e-12 60.8
MS.gene39549.t1 MTR_6g465690 44.928 69 37 1 25 93 51 118 6.33e-12 60.5
MS.gene39549.t1 MTR_6g466000 40.909 88 49 2 6 93 28 112 6.60e-12 60.1
MS.gene39549.t1 MTR_6g465430 52.632 57 26 1 37 93 54 109 7.05e-12 60.5
MS.gene39549.t1 MTR_6g465530 46.377 69 36 1 25 93 21 88 7.35e-12 59.7
MS.gene39549.t1 MTR_6g465450 46.875 64 33 1 30 93 47 109 8.25e-12 60.1
MS.gene39549.t1 MTR_6g465460 52.632 57 26 1 37 93 54 109 1.00e-11 60.1
MS.gene39549.t1 MTR_3g105480 38.750 80 43 3 39 116 33 108 1.05e-11 59.7
MS.gene39549.t1 MTR_6g465420 50.877 57 27 1 37 93 54 109 1.92e-11 59.3
MS.gene39549.t1 MTR_8g023680 64.286 42 14 1 37 78 116 156 2.04e-11 59.7
MS.gene39549.t1 MTR_5g008590 56.897 58 24 1 37 94 11 67 2.12e-11 58.2
MS.gene39549.t1 MTR_5g010940 43.478 69 34 1 30 93 47 115 2.19e-11 58.9
MS.gene39549.t1 MTR_5g010930 46.774 62 28 1 37 93 49 110 3.27e-11 58.2
MS.gene39549.t1 MTR_5g009410 63.077 65 23 1 31 95 111 174 3.43e-11 58.9
MS.gene39549.t1 MTR_5g010910 43.478 69 34 1 30 93 42 110 3.85e-11 58.2
MS.gene39549.t1 MTR_3g112440 53.030 66 29 2 37 102 39 102 9.17e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39549.t1 AT5G47220 63.750 80 28 1 16 94 95 174 1.18e-32 114
MS.gene39549.t1 AT4G17500 77.419 62 14 0 33 94 144 205 2.31e-31 112
MS.gene39549.t1 AT2G44840 50.467 107 41 1 1 95 44 150 1.85e-30 108
MS.gene39549.t1 AT3G23240 52.632 95 41 2 1 93 46 138 8.31e-29 104
MS.gene39549.t1 AT5G43410 72.581 62 17 0 37 98 15 76 8.49e-29 101
MS.gene39549.t1 AT5G47230 72.581 62 17 0 33 94 152 213 7.43e-28 103
MS.gene39549.t1 AT3G23230 66.154 65 22 0 33 97 16 80 1.70e-27 98.6
MS.gene39549.t1 AT5G51190 70.492 61 18 0 34 94 69 129 2.06e-27 100
MS.gene39549.t1 AT4G17490 70.968 62 18 0 33 94 133 194 4.35e-27 101
MS.gene39549.t1 AT2G33710 66.667 69 22 1 26 94 59 126 7.91e-27 99.4
MS.gene39549.t1 AT2G33710 66.667 69 22 1 26 94 59 126 8.03e-27 99.4
MS.gene39549.t1 AT2G31230 54.444 90 34 1 12 94 54 143 1.03e-26 99.4
MS.gene39549.t1 AT5G13330 53.409 88 37 2 20 107 25 108 1.81e-26 98.2
MS.gene39549.t1 AT5G61600 59.155 71 29 0 35 105 86 156 7.36e-26 97.4
MS.gene39549.t1 AT4G18450 71.875 64 17 1 31 93 104 167 1.49e-25 97.8
MS.gene39549.t1 AT3G61630 54.762 84 35 2 29 110 97 179 5.53e-25 96.3
MS.gene39549.t1 AT5G61890 52.128 94 36 3 14 107 71 155 9.60e-25 94.4
MS.gene39549.t1 AT5G07580 60.000 65 26 0 32 96 105 169 2.14e-24 92.8
MS.gene39549.t1 AT3G23220 67.143 70 23 0 28 97 5 74 1.69e-23 88.6
MS.gene39549.t1 AT3G16770 61.333 75 27 2 21 94 62 135 2.45e-23 90.9
MS.gene39549.t1 AT2G47520 65.079 63 21 1 33 95 46 107 5.02e-23 88.2
MS.gene39549.t1 AT5G53290 60.000 75 27 2 19 93 109 180 5.05e-23 91.7
MS.gene39549.t1 AT4G28140 62.121 66 24 1 32 97 138 202 1.61e-22 89.4
MS.gene39549.t1 AT2G22200 49.485 97 44 2 3 95 33 128 1.98e-22 88.6
MS.gene39549.t1 AT1G03800 59.722 72 25 2 23 94 42 109 3.02e-22 87.8
MS.gene39549.t1 AT4G27950 65.000 60 20 1 34 93 115 173 3.14e-22 89.4
MS.gene39549.t1 AT4G39780 48.387 93 47 1 3 95 59 150 5.11e-22 87.8
MS.gene39549.t1 AT1G72360 65.079 63 21 1 37 99 25 86 1.19e-21 85.5
MS.gene39549.t1 AT1G04370 70.968 62 18 0 37 98 20 81 1.47e-21 83.6
MS.gene39549.t1 AT1G72360 54.430 79 35 1 21 99 58 135 1.96e-21 86.3
MS.gene39549.t1 AT1G22190 59.722 72 26 2 23 94 71 139 2.14e-21 85.9
MS.gene39549.t1 AT4G11140 63.333 60 21 1 35 94 85 143 3.03e-21 85.9
MS.gene39549.t1 AT1G36060 56.410 78 30 2 17 94 126 199 3.10e-21 86.3
MS.gene39549.t1 AT1G72360 65.079 63 21 1 37 99 76 137 4.23e-21 85.1
MS.gene39549.t1 AT3G15210 60.811 74 28 1 33 106 21 93 4.25e-21 84.3
MS.gene39549.t1 AT3G20310 56.164 73 31 1 31 103 21 92 5.74e-21 84.7
MS.gene39549.t1 AT2G20880 63.492 63 22 1 35 97 185 246 6.19e-21 85.9
MS.gene39549.t1 AT1G78080 66.667 60 19 1 35 94 150 208 1.06e-20 85.1
MS.gene39549.t1 AT1G28370 68.966 58 17 1 37 94 20 76 1.07e-20 82.0
MS.gene39549.t1 AT5G65130 65.000 60 20 1 35 94 109 167 1.24e-20 84.3
MS.gene39549.t1 AT5G65130 65.000 60 20 1 35 94 111 169 1.37e-20 84.3
MS.gene39549.t1 AT1G28370 68.966 58 17 1 37 94 58 114 1.83e-20 82.4
MS.gene39549.t1 AT1G28360 52.632 76 35 1 26 101 1 75 1.91e-20 82.0
MS.gene39549.t1 AT2G46310 48.387 93 43 3 23 113 87 176 2.66e-20 83.6
MS.gene39549.t1 AT5G44210 64.516 62 21 1 33 94 29 89 3.60e-20 81.6
MS.gene39549.t1 AT1G50640 60.606 66 25 1 31 96 22 86 4.31e-20 82.0
MS.gene39549.t1 AT3G57600 62.712 59 21 1 37 95 28 85 4.37e-20 82.8
MS.gene39549.t1 AT1G06160 69.355 62 19 0 35 96 80 141 4.78e-20 82.0
MS.gene39549.t1 AT1G22985 47.778 90 43 2 31 116 69 158 1.19e-19 79.3
MS.gene39549.t1 AT4G23750 56.923 65 27 1 29 93 114 177 1.68e-19 82.0
MS.gene39549.t1 AT4G23750 56.923 65 27 1 29 93 114 177 1.68e-19 82.0
MS.gene39549.t1 AT1G71450 51.351 74 34 2 37 110 24 95 3.06e-19 78.6
MS.gene39549.t1 AT5G25810 44.000 100 46 4 21 116 16 109 3.37e-19 79.3
MS.gene39549.t1 AT3G11020 58.333 72 27 2 36 107 77 145 5.84e-19 80.5
MS.gene39549.t1 AT1G01250 43.373 83 46 1 29 111 37 118 7.07e-19 78.2
MS.gene39549.t1 AT3G50260 42.697 89 44 2 30 118 14 95 8.18e-19 77.0
MS.gene39549.t1 AT4G13620 62.500 64 23 1 31 94 226 288 1.08e-18 80.1
MS.gene39549.t1 AT5G05410 56.944 72 28 2 36 107 78 146 1.11e-18 79.3
MS.gene39549.t1 AT4G06746 42.353 85 48 1 34 118 33 116 1.12e-18 76.6
MS.gene39549.t1 AT5G61590 62.903 62 23 0 35 96 105 166 1.40e-18 77.4
MS.gene39549.t1 AT5G05410 56.944 72 28 2 36 107 78 146 1.48e-18 79.3
MS.gene39549.t1 AT2G35700 56.452 62 26 1 33 94 41 101 2.32e-18 76.6
MS.gene39549.t1 AT5G13910 52.381 84 32 3 29 105 6 88 3.29e-18 76.6
MS.gene39549.t1 AT4G16750 51.471 68 32 1 27 94 30 96 3.59e-18 75.9
MS.gene39549.t1 AT1G75490 49.315 73 36 1 37 109 42 113 3.97e-18 76.3
MS.gene39549.t1 AT4G31060 54.688 64 28 1 33 96 23 85 4.32e-18 75.9
MS.gene39549.t1 AT1G24590 69.492 59 13 2 37 93 58 113 5.18e-18 77.8
MS.gene39549.t1 AT5G07310 66.667 63 20 1 32 94 87 148 5.78e-18 77.0
MS.gene39549.t1 AT1G12980 66.667 57 18 1 37 93 57 112 7.02e-18 77.4
MS.gene39549.t1 AT5G50080 56.627 83 35 1 14 96 54 135 7.65e-18 75.9
MS.gene39549.t1 AT5G50080 56.627 83 35 1 14 96 63 144 9.75e-18 75.9
MS.gene39549.t1 AT4G32800 49.275 69 34 1 27 95 9 76 1.53e-17 75.1
MS.gene39549.t1 AT5G18560 56.923 65 27 1 29 93 47 110 1.71e-17 76.6
MS.gene39549.t1 AT2G40340 58.333 60 24 1 36 95 13 71 2.01e-17 75.9
MS.gene39549.t1 AT2G40340 58.333 60 24 1 36 95 13 71 2.01e-17 75.9
MS.gene39549.t1 AT2G40340 58.333 60 24 1 36 95 13 71 2.01e-17 75.9
MS.gene39549.t1 AT4G36900 50.794 63 30 1 33 95 26 87 2.07e-17 74.3
MS.gene39549.t1 AT1G28160 48.864 88 43 2 32 118 34 120 2.35e-17 75.1
MS.gene39549.t1 AT2G40340 53.425 73 31 2 36 106 71 142 3.06e-17 75.5
MS.gene39549.t1 AT2G40340 58.333 60 24 1 36 95 71 129 3.37e-17 75.9
MS.gene39549.t1 AT2G40340 58.333 60 24 1 36 95 78 136 3.70e-17 75.9
MS.gene39549.t1 AT2G23340 42.353 85 47 2 35 118 26 109 3.86e-17 73.2
MS.gene39549.t1 AT1G15360 55.556 63 27 1 32 94 2 63 3.96e-17 73.6
MS.gene39549.t1 AT1G15360 55.556 63 27 1 32 94 2 63 3.96e-17 73.6
MS.gene39549.t1 AT5G11590 45.570 79 42 1 33 111 47 124 4.29e-17 74.3
MS.gene39549.t1 AT2G25820 53.968 63 26 2 37 97 26 87 4.67e-17 73.9
MS.gene39549.t1 AT3G16280 48.000 75 38 1 37 111 61 134 7.09e-17 73.6
MS.gene39549.t1 AT3G16280 48.000 75 38 1 37 111 79 152 8.10e-17 73.9
MS.gene39549.t1 AT3G60490 37.624 101 57 2 16 111 45 144 1.26e-16 73.2
MS.gene39549.t1 AT1G43160 65.625 64 21 1 33 96 57 119 1.33e-16 72.0
MS.gene39549.t1 AT1G46768 42.353 85 46 2 12 94 4 87 1.75e-16 70.5
MS.gene39549.t1 AT1G46768 52.459 61 28 1 34 94 28 87 1.82e-16 70.9
MS.gene39549.t1 AT1G46768 52.459 61 28 1 34 94 28 87 1.82e-16 70.9
MS.gene39549.t1 AT4G34410 62.687 67 23 2 36 101 135 200 1.96e-16 72.8
MS.gene39549.t1 AT5G25190 46.914 81 38 2 33 113 4 79 2.02e-16 71.2
MS.gene39549.t1 AT2G44940 53.226 62 28 1 33 94 96 156 2.68e-16 72.8
MS.gene39549.t1 AT5G67190 41.860 86 45 2 33 118 17 97 2.73e-16 71.2
MS.gene39549.t1 AT1G71130 54.167 72 27 2 26 93 70 139 2.82e-16 70.5
MS.gene39549.t1 AT5G19790 51.282 78 36 2 27 104 13 88 4.77e-16 71.6
MS.gene39549.t1 AT5G25390 46.914 81 39 2 32 109 2 81 9.31e-16 69.7
MS.gene39549.t1 AT5G18450 55.932 59 25 1 37 95 34 91 1.03e-15 71.2
MS.gene39549.t1 AT5G64750 67.308 52 16 1 45 96 193 243 1.06e-15 72.0
MS.gene39549.t1 AT5G25390 45.679 81 37 2 32 109 2 78 1.83e-15 68.9
MS.gene39549.t1 AT2G36450 47.945 73 36 2 37 108 15 86 1.97e-15 68.9
MS.gene39549.t1 AT1G74930 43.590 78 37 2 19 96 9 79 3.68e-15 68.6
MS.gene39549.t1 AT1G12890 53.030 66 30 1 28 93 12 76 4.68e-15 68.6
MS.gene39549.t1 AT1G53910 64.789 71 23 2 27 96 112 181 7.71e-15 69.3
MS.gene39549.t1 AT1G53910 64.789 71 23 2 27 96 114 183 7.92e-15 69.3
MS.gene39549.t1 AT1G53910 64.789 71 23 2 27 96 114 183 7.92e-15 69.3
MS.gene39549.t1 AT2G40350 52.542 59 27 1 36 94 73 130 1.08e-14 66.6
MS.gene39549.t1 AT1G22810 41.758 91 44 3 21 109 4 87 1.13e-14 65.9
MS.gene39549.t1 AT2G40350 52.542 59 27 1 36 94 66 123 1.14e-14 66.2
MS.gene39549.t1 AT1G21910 51.724 58 27 1 37 94 50 106 1.41e-14 67.4
MS.gene39549.t1 AT1G19210 42.466 73 37 2 26 96 1 70 2.21e-14 66.2
MS.gene39549.t1 AT4G25490 56.140 57 24 1 37 93 48 103 3.47e-14 66.2
MS.gene39549.t1 AT4G25480 56.140 57 24 1 37 93 51 106 5.55e-14 65.5
MS.gene39549.t1 AT4G25470 56.140 57 24 1 37 93 51 106 6.04e-14 65.5
MS.gene39549.t1 AT5G21960 51.724 58 27 1 37 94 7 63 7.44e-14 65.5
MS.gene39549.t1 AT3G14230 60.526 76 28 2 27 101 113 187 1.13e-13 66.2
MS.gene39549.t1 AT3G14230 60.526 76 28 2 27 101 112 186 1.14e-13 66.2
MS.gene39549.t1 AT1G71520 40.909 88 37 4 37 118 14 92 1.15e-13 63.5
MS.gene39549.t1 AT3G14230 60.526 76 28 2 27 101 117 191 1.30e-13 65.9
MS.gene39549.t1 AT5G51990 52.632 57 26 1 37 93 54 109 5.86e-13 63.2
MS.gene39549.t1 AT1G77200 52.459 61 28 1 35 95 41 100 7.84e-13 62.8
MS.gene39549.t1 AT1G68550 58.000 50 20 1 29 78 105 153 1.28e-12 63.2
MS.gene39549.t1 AT1G68550 58.000 50 20 1 29 78 105 153 1.28e-12 63.2
MS.gene39549.t1 AT1G53170 59.091 66 26 1 28 93 22 86 2.00e-12 60.8
MS.gene39549.t1 AT1G64380 58.750 80 30 2 31 108 130 208 2.75e-12 62.0
MS.gene39549.t1 AT1G68840 45.833 72 35 2 23 93 50 118 6.23e-12 61.2
MS.gene39549.t1 AT1G68840 45.833 72 35 2 23 93 50 118 6.23e-12 61.2
MS.gene39549.t1 AT2G38340 53.521 71 26 2 37 100 70 140 8.31e-12 60.1
MS.gene39549.t1 AT1G63030 32.353 102 67 2 9 110 2 101 5.60e-11 57.0
MS.gene39549.t1 AT1G63030 32.353 102 67 2 9 110 2 101 5.60e-11 57.0

Find 24 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAGCTTCCTTAAGAATT+TGG 0.176380 4.2:-23279692 None:intergenic
CCTTGAACTCAAATCACTCT+TGG 0.339899 4.2:-23280000 None:intergenic
ATTCGTGACTCGAAGCAAAA+TGG 0.384421 4.2:+23279834 MS.gene39549:CDS
GGTTATATACCAAATTCTTA+AGG 0.414912 4.2:+23279683 MS.gene39549:CDS
CCAAGAGTGATTTGAGTTCA+AGG 0.429785 4.2:+23280000 MS.gene39549:CDS
GTGCAAGAGAGGCATCAAAA+TGG 0.442976 4.2:+23279732 MS.gene39549:CDS
AGGCGTAAGGCATCGACGGT+GGG 0.473861 4.2:+23279794 MS.gene39549:CDS
AGCAAAATGGAGCTAGGGTA+TGG 0.479507 4.2:+23279847 MS.gene39549:CDS
GCGTAAGGCATCGACGGTGG+GGG 0.515694 4.2:+23279796 MS.gene39549:CDS
ATAAAGCTGCTTTCAGAATG+AGG 0.517117 4.2:+23279913 MS.gene39549:CDS
ACTCGAAGCAAAATGGAGCT+AGG 0.518024 4.2:+23279841 MS.gene39549:CDS
GGCGTAAGGCATCGACGGTG+GGG 0.545453 4.2:+23279795 MS.gene39549:CDS
TTTCAGAATGAGGGGTGCTA+AGG 0.550876 4.2:+23279923 MS.gene39549:CDS
GAGGCGTAAGGCATCGACGG+TGG 0.553788 4.2:+23279793 MS.gene39549:CDS
TACATTTCAAACAGCTGAAG+AGG 0.558185 4.2:+23279875 MS.gene39549:CDS
TAAAGCTGCTTTCAGAATGA+GGG 0.568686 4.2:+23279914 MS.gene39549:CDS
CTCGAAGCAAAATGGAGCTA+GGG 0.572765 4.2:+23279842 MS.gene39549:CDS
GCAACACAATGGTGCAAGAG+AGG 0.592167 4.2:+23279721 MS.gene39549:CDS
AATGGAGCTAGGGTATGGCT+TGG 0.594507 4.2:+23279852 MS.gene39549:CDS
AGAAGAACTACAGAGGCGTA+AGG 0.610848 4.2:+23279781 MS.gene39549:CDS
ACAGAGGCGTAAGGCATCGA+CGG 0.641932 4.2:+23279790 MS.gene39549:CDS
AAAGCTGCTTTCAGAATGAG+GGG 0.658658 4.2:+23279915 MS.gene39549:CDS
ATCATGTGGGAAATTCAACA+AGG 0.698701 4.2:-23279946 None:intergenic
TAATGAACTTAGCAACACAA+TGG 0.708706 4.2:+23279710 MS.gene39549:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTAAAAAGAAGAACTACAG+AGG + chr4.2:23279774-23279793 MS.gene39549:CDS 25.0%
! GGTTATATACCAAATTCTTA+AGG + chr4.2:23279683-23279702 MS.gene39549:CDS 25.0%
!! TCTGTAGTTCTTCTTTTTAA+TGG - chr4.2:23279775-23279794 None:intergenic 25.0%
ATAGAAGCAGAAAAATCATG+TGG - chr4.2:23279963-23279982 None:intergenic 30.0%
TAATGAACTTAGCAACACAA+TGG + chr4.2:23279710-23279729 MS.gene39549:CDS 30.0%
TAGAAGCAGAAAAATCATGT+GGG - chr4.2:23279962-23279981 None:intergenic 30.0%
! CATTAGCTTCCTTAAGAATT+TGG - chr4.2:23279695-23279714 None:intergenic 30.0%
ATCATGTGGGAAATTCAACA+AGG - chr4.2:23279949-23279968 None:intergenic 35.0%
TACATTTCAAACAGCTGAAG+AGG + chr4.2:23279875-23279894 MS.gene39549:CDS 35.0%
! ATAAAGCTGCTTTCAGAATG+AGG + chr4.2:23279913-23279932 MS.gene39549:CDS 35.0%
! TAAAGCTGCTTTCAGAATGA+GGG + chr4.2:23279914-23279933 MS.gene39549:CDS 35.0%
ATTCGTGACTCGAAGCAAAA+TGG + chr4.2:23279834-23279853 MS.gene39549:CDS 40.0%
CCTTGAACTCAAATCACTCT+TGG - chr4.2:23280003-23280022 None:intergenic 40.0%
! AAAGCTGCTTTCAGAATGAG+GGG + chr4.2:23279915-23279934 MS.gene39549:CDS 40.0%
! CCAAGAGTGATTTGAGTTCA+AGG + chr4.2:23280000-23280019 MS.gene39549:CDS 40.0%
ACTCGAAGCAAAATGGAGCT+AGG + chr4.2:23279841-23279860 MS.gene39549:CDS 45.0%
AGCAAAATGGAGCTAGGGTA+TGG + chr4.2:23279847-23279866 MS.gene39549:CDS 45.0%
CTCGAAGCAAAATGGAGCTA+GGG + chr4.2:23279842-23279861 MS.gene39549:CDS 45.0%
! AGAAGAACTACAGAGGCGTA+AGG + chr4.2:23279781-23279800 MS.gene39549:CDS 45.0%
!! GTGCAAGAGAGGCATCAAAA+TGG + chr4.2:23279732-23279751 MS.gene39549:CDS 45.0%
!! TTTCAGAATGAGGGGTGCTA+AGG + chr4.2:23279923-23279942 MS.gene39549:CDS 45.0%
AATGGAGCTAGGGTATGGCT+TGG + chr4.2:23279852-23279871 MS.gene39549:CDS 50.0%
GCAACACAATGGTGCAAGAG+AGG + chr4.2:23279721-23279740 MS.gene39549:CDS 50.0%
!! ACAGAGGCGTAAGGCATCGA+CGG + chr4.2:23279790-23279809 MS.gene39549:CDS 55.0%
!! AGGCGTAAGGCATCGACGGT+GGG + chr4.2:23279794-23279813 MS.gene39549:CDS 60.0%
!! GAGGCGTAAGGCATCGACGG+TGG + chr4.2:23279793-23279812 MS.gene39549:CDS 65.0%
!! GCGTAAGGCATCGACGGTGG+GGG + chr4.2:23279796-23279815 MS.gene39549:CDS 65.0%
!! GGCGTAAGGCATCGACGGTG+GGG + chr4.2:23279795-23279814 MS.gene39549:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 23279681 23280037 23279681 ID=MS.gene39549
chr4.2 mRNA 23279681 23280037 23279681 ID=MS.gene39549.t1;Parent=MS.gene39549
chr4.2 exon 23279681 23280037 23279681 ID=MS.gene39549.t1.exon1;Parent=MS.gene39549.t1
chr4.2 CDS 23279681 23280037 23279681 ID=cds.MS.gene39549.t1;Parent=MS.gene39549.t1
Gene Sequence

>MS.gene39549

ATGGTTATATACCAAATTCTTAAGGAAGCTAATGAACTTAGCAACACAATGGTGCAAGAGAGGCATCAAAATGGCTTAGAACAGAGCAAGACCATTAAAAAGAAGAACTACAGAGGCGTAAGGCATCGACGGTGGGGGAAATATGCAGCAGAGATTCGTGACTCGAAGCAAAATGGAGCTAGGGTATGGCTTGGTACATTTCAAACAGCTGAAGAGGCTGCAGTTGCTTATGATAAAGCTGCTTTCAGAATGAGGGGTGCTAAGGCCTTGTTGAATTTCCCACATGATTTTTCTGCTTCTATACAATTGACTTCAACGTCCAAGAGTGATTTGAGTTCAAGGAAAATAAACAAATAA
Protein sequence

>MS.gene39549.t1

MVIYQILKEANELSNTMVQERHQNGLEQSKTIKKKNYRGVRHRRWGKYAAEIRDSKQNGARVWLGTFQTAEEAAVAYDKAAFRMRGAKALLNFPHDFSASIQLTSTSKSDLSSRKINK