AlfalfaGEDB | MS.gene052456

M. sativa cultivar XinJiangDaYe / MS.gene052456

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052456.t1	RHN52894.1	73.2	56	15	0	1	56	60	115	9.40E-15	89.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052456.t1	A0A396HLX6	73.2	56	15	0	1	56	60	115	6.8e-15	89.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052456	MS.gene053383	0.805882	1.08E-49	-1.69E-46
MS.gene052456	MS.gene054801	0.818941	1.51E-52	-1.69E-46
MS.gene052456	MS.gene055359	0.861215	1.20E-63	-1.69E-46
MS.gene052456	MS.gene059632	0.803453	3.46E-49	-1.69E-46
MS.gene052456	MS.gene061336	0.821654	3.60E-53	-1.69E-46
MS.gene052456	MS.gene061337	0.835151	1.98E-56	-1.69E-46
MS.gene052456	MS.gene065863	0.832114	1.14E-55	-1.69E-46
MS.gene052456	MS.gene065865	0.810904	9.14E-51	-1.69E-46
MS.gene052456	MS.gene067101	0.825677	4.11E-54	-1.69E-46
MS.gene052456	MS.gene067276	0.839113	1.92E-57	-1.69E-46
MS.gene052456	MS.gene072320	0.801229	9.94E-49	-1.69E-46
MS.gene052456	MS.gene074520	0.820024	8.53E-53	-1.69E-46
MS.gene052456	MS.gene08896	0.850406	1.73E-60	-1.69E-46
MS.gene052456	MS.gene09451	0.825828	3.79E-54	-1.69E-46
MS.gene052456	MS.gene20964	0.8051	1.57E-49	-1.69E-46
MS.gene052456	MS.gene22109	0.813085	3.06E-51	-1.69E-46
MS.gene052456	MS.gene22900	0.803099	4.10E-49	-1.69E-46
MS.gene052456	MS.gene39549	0.813332	2.70E-51	-1.69E-46
MS.gene052456	MS.gene44743	0.803526	3.34E-49	-1.69E-46
MS.gene052456	MS.gene51424	0.815011	1.15E-51	-1.69E-46
MS.gene052456	MS.gene51459	0.824733	6.88E-54	-1.69E-46
MS.gene052456	MS.gene53586	0.811598	6.46E-51	-1.69E-46
MS.gene052456	MS.gene56024	0.816322	5.87E-52	-1.69E-46
MS.gene052456	MS.gene60245	0.833998	3.86E-56	-1.69E-46
MS.gene052456	MS.gene61665	0.840304	9.42E-58	-1.69E-46
MS.gene052456	MS.gene62049	0.849124	3.95E-60	-1.69E-46
MS.gene052456	MS.gene64249	0.81056	1.08E-50	-1.69E-46
MS.gene052456	MS.gene67194	0.837371	5.41E-57	-1.69E-46
MS.gene052456	MS.gene72268	0.822607	2.16E-53	-1.69E-46
MS.gene052456	MS.gene73229	0.807825	4.18E-50	-1.69E-46
MS.gene052456	MS.gene73522	0.800485	1.41E-48	-1.69E-46
MS.gene052456	MS.gene73555	0.809769	1.61E-50	-1.69E-46
MS.gene052456	MS.gene81735	0.838168	3.37E-57	-1.69E-46
MS.gene052456	MS.gene90334	0.81785	2.66E-52	-1.69E-46
MS.gene052456	MS.gene91805	0.836406	9.53E-57	-1.69E-46
MS.gene052456	MS.gene97797	0.809641	1.71E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052456.t1	MTR_6g086745	73.529	34	9	0	1	34	100	133	3.67e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACTGACTCTTCATCCCTTT+TGG	0.233722	6.1:-75574611	None:intergenic
AGAGTCAGTCAAAGGTTCTT+TGG	0.292750	6.1:+75574624	MS.gene052456:CDS
GAGTCAGTCAAAGGTTCTTT+GGG	0.376370	6.1:+75574625	MS.gene052456:CDS
AATTCATCATACGTTATTCC+AGG	0.389672	6.1:-75574691	None:intergenic
TTCTATTCACATCTTCCAAA+AGG	0.390556	6.1:+75574596	MS.gene052456:CDS
TTCATGATTGATGCTTGTTA+CGG	0.446394	6.1:+75574538	MS.gene052456:CDS
TCTATTCACATCTTCCAAAA+GGG	0.515999	6.1:+75574597	MS.gene052456:CDS
TGATTGATGCTTGTTACGGA+GGG	0.554799	6.1:+75574542	MS.gene052456:CDS
ATGATTGATGCTTGTTACGG+AGG	0.579217	6.1:+75574541	MS.gene052456:CDS
TAGAATCAAGTTAAGAGAGT+GGG	0.593682	6.1:+75574811	MS.gene052456:intron
ATTCATCATACGTTATTCCA+GGG	0.594580	6.1:-75574690	None:intergenic
ATGTGAATAGAACAACTTTG+TGG	0.598030	6.1:-75574585	None:intergenic
AGGGATGAAGAGTCAGTCAA+AGG	0.601689	6.1:+75574616	MS.gene052456:CDS
GATTGTGTTAATGATTACCC+TGG	0.604085	6.1:+75574673	MS.gene052456:CDS
ATAGAATCAAGTTAAGAGAG+TGG	0.627109	6.1:+75574810	MS.gene052456:intron
GATTGATGCTTGTTACGGAG+GGG	0.634428	6.1:+75574543	MS.gene052456:CDS
TGAATAGAACAACTTTGTGG+TGG	0.649665	6.1:-75574582	None:intergenic
GTGTTAGACCAAATGGCTGA+CGG	0.654978	6.1:+75574502	None:intergenic
GAATAGAACAACTTTGTGGT+GGG	0.689752	6.1:-75574581	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAAAGGATCCAAATATTATA+TGG	+	chr6.1:75574718-75574737	MS.gene052456:intron	25.0%
!!!	AGAATTTTTTATCTCATCAG+TGG	-	chr6.1:75574779-75574798	None:intergenic	25.0%
	AATTCATCATACGTTATTCC+AGG	-	chr6.1:75574694-75574713	None:intergenic	30.0%
	ATAGAATCAAGTTAAGAGAG+TGG	+	chr6.1:75574810-75574829	MS.gene052456:intron	30.0%
	ATGTGAATAGAACAACTTTG+TGG	-	chr6.1:75574588-75574607	None:intergenic	30.0%
	ATTCATCATACGTTATTCCA+GGG	-	chr6.1:75574693-75574712	None:intergenic	30.0%
	CAAATATTATATGGTCAGCA+TGG	+	chr6.1:75574727-75574746	MS.gene052456:intron	30.0%
	CATGCTGACCATATAATATT+TGG	-	chr6.1:75574729-75574748	None:intergenic	30.0%
	CGTATGATGAATTGATACAA+AGG	+	chr6.1:75574701-75574720	MS.gene052456:intron	30.0%
	TAGAATCAAGTTAAGAGAGT+GGG	+	chr6.1:75574811-75574830	MS.gene052456:intron	30.0%
	TCTATTCACATCTTCCAAAA+GGG	+	chr6.1:75574597-75574616	MS.gene052456:CDS	30.0%
	TTCTATTCACATCTTCCAAA+AGG	+	chr6.1:75574596-75574615	MS.gene052456:CDS	30.0%
!	TTCATGATTGATGCTTGTTA+CGG	+	chr6.1:75574538-75574557	MS.gene052456:CDS	30.0%
	GAATAGAACAACTTTGTGGT+GGG	-	chr6.1:75574584-75574603	None:intergenic	35.0%
	GATTGTGTTAATGATTACCC+TGG	+	chr6.1:75574673-75574692	MS.gene052456:CDS	35.0%
	TGAATAGAACAACTTTGTGG+TGG	-	chr6.1:75574585-75574604	None:intergenic	35.0%
	TTATCTCATCAGTGGCATAA+AGG	-	chr6.1:75574771-75574790	None:intergenic	35.0%
!!!	TCTGCATCTAGCTTTTTCAT+TGG	-	chr6.1:75574855-75574874	None:intergenic	35.0%
	AGAGTCAGTCAAAGGTTCTT+TGG	+	chr6.1:75574624-75574643	MS.gene052456:CDS	40.0%
	GAGTCAGTCAAAGGTTCTTT+GGG	+	chr6.1:75574625-75574644	MS.gene052456:CDS	40.0%
!	ATGATTGATGCTTGTTACGG+AGG	+	chr6.1:75574541-75574560	MS.gene052456:CDS	40.0%
!	TGATTGATGCTTGTTACGGA+GGG	+	chr6.1:75574542-75574561	MS.gene052456:CDS	40.0%
	AGGGATGAAGAGTCAGTCAA+AGG	+	chr6.1:75574616-75574635	MS.gene052456:CDS	45.0%
	CTCATCAGTGGCATAAAGGT+CGG	-	chr6.1:75574767-75574786	None:intergenic	45.0%
	TCATCAGTGGCATAAAGGTC+GGG	-	chr6.1:75574766-75574785	None:intergenic	45.0%
!	GATTGATGCTTGTTACGGAG+GGG	+	chr6.1:75574543-75574562	MS.gene052456:CDS	45.0%
!!	GACTGACTCTTCATCCCTTT+TGG	-	chr6.1:75574614-75574633	None:intergenic	45.0%
	GTGGCATAAAGGTCGGGTAG+TGG	-	chr6.1:75574760-75574779	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	75574514	75574895	75574514	ID=MS.gene052456
chr6.1	mRNA	75574514	75574895	75574514	ID=MS.gene052456.t1;Parent=MS.gene052456
chr6.1	exon	75574514	75574701	75574514	ID=MS.gene052456.t1.exon1;Parent=MS.gene052456.t1
chr6.1	CDS	75574514	75574701	75574514	ID=cds.MS.gene052456.t1;Parent=MS.gene052456.t1
chr6.1	exon	75574814	75574895	75574814	ID=MS.gene052456.t1.exon2;Parent=MS.gene052456.t1
chr6.1	CDS	75574814	75574895	75574814	ID=cds.MS.gene052456.t1;Parent=MS.gene052456.t1

Gene Sequence

>MS.gene052456

ATGGCTGACGGCGTCAAGATAATTTTCATGATTGATGCTTGTTACGGAGGGGAGTTTATTGAACTAGCCCACCACAAAGTTGTTCTATTCACATCTTCCAAAAGGGATGAAGAGTCAGTCAAAGGTTCTTTGGGTTCTGTGTTTACGAATGCGTTTGTTGATTGTGTTAATGATTACCCTGGAATAACGTATGATGAATTGATACAAAGGATCCAAATATTATATGGTCAGCATGGAAGCAAACCACTACCCGACCTTTATGCCACTGATGAGATAAAAAATTCTATTTTTATTCTATAGAATCAAGTTAAGAGAGTGGGATAGAGAGATGTTTGACTCCAATGAAAAAGCTAGATGCAGAAGCTTGTACTATTTCTCCTAG

Protein sequence

>MS.gene052456.t1

MADGVKIIFMIDACYGGEFIELAHHKVVLFTSSKRDEESVKGSLGSVFTNAFVDCVNDYPGITIKLREWDREMFDSNEKARCRSLYYFS