Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39590.t1 | XP_003608059.2 | 97.7 | 439 | 9 | 1 | 1 | 438 | 1 | 439 | 2.50E-241 | 844.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39590.t1 | O52225 | 35.4 | 274 | 144 | 5 | 109 | 382 | 13 | 253 | 1.4e-38 | 162.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39590.t1 | G7JNK8 | 97.7 | 439 | 9 | 1 | 1 | 438 | 1 | 439 | 1.8e-241 | 844.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049427 | MS.gene39590 | 0.802706 | 4.94E-49 | -1.69E-46 |
| MS.gene049627 | MS.gene39590 | 0.824895 | 6.30E-54 | -1.69E-46 |
| MS.gene051694 | MS.gene39590 | 0.830539 | 2.77E-55 | -1.69E-46 |
| MS.gene054802 | MS.gene39590 | 0.805667 | 1.20E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene39590 | 0.802339 | 5.88E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene39590 | 0.810977 | 8.81E-51 | -1.69E-46 |
| MS.gene056452 | MS.gene39590 | 0.830034 | 3.69E-55 | -1.69E-46 |
| MS.gene05883 | MS.gene39590 | 0.800264 | 1.56E-48 | -1.69E-46 |
| MS.gene059960 | MS.gene39590 | 0.815542 | 8.76E-52 | -1.69E-46 |
| MS.gene061200 | MS.gene39590 | 0.816811 | 4.56E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39590.t1 | MTR_4g087250 | 97.722 | 439 | 9 | 1 | 1 | 438 | 1 | 439 | 0.0 | 880 |
| MS.gene39590.t1 | MTR_6g033525 | 28.019 | 207 | 132 | 4 | 191 | 381 | 108 | 313 | 3.16e-17 | 83.2 |
| MS.gene39590.t1 | MTR_6g033525 | 28.488 | 172 | 108 | 3 | 191 | 348 | 108 | 278 | 9.85e-17 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39590.t1 | AT3G52050 | 64.583 | 432 | 136 | 5 | 10 | 437 | 5 | 423 | 0.0 | 538 |
| MS.gene39590.t1 | AT3G52050 | 64.583 | 432 | 136 | 5 | 10 | 437 | 5 | 423 | 0.0 | 538 |
| MS.gene39590.t1 | AT3G52050 | 64.583 | 432 | 136 | 5 | 10 | 437 | 28 | 446 | 0.0 | 537 |
| MS.gene39590.t1 | AT3G52050 | 63.657 | 432 | 128 | 7 | 10 | 437 | 5 | 411 | 0.0 | 521 |
| MS.gene39590.t1 | AT3G52050 | 62.731 | 432 | 126 | 6 | 10 | 437 | 5 | 405 | 0.0 | 514 |
| MS.gene39590.t1 | AT3G52050 | 62.731 | 432 | 126 | 6 | 10 | 437 | 5 | 405 | 0.0 | 514 |
| MS.gene39590.t1 | AT3G52050 | 62.731 | 432 | 126 | 6 | 10 | 437 | 28 | 428 | 0.0 | 513 |
| MS.gene39590.t1 | AT3G52050 | 60.714 | 364 | 104 | 7 | 10 | 369 | 28 | 356 | 6.11e-139 | 402 |
| MS.gene39590.t1 | AT1G34380 | 28.649 | 185 | 121 | 5 | 191 | 366 | 120 | 302 | 2.35e-14 | 74.3 |
| MS.gene39590.t1 | AT1G34380 | 28.649 | 185 | 121 | 5 | 191 | 366 | 120 | 302 | 4.00e-14 | 73.9 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGATATGATTGCACTTTC+TGG | 0.151527 | 4.2:+24076165 | MS.gene39590:CDS |
| CTTGCAGATTATTGATGTTC+TGG | 0.236084 | 4.2:+24071390 | MS.gene39590:intron |
| GCAGCTCCGTAACTAATTTC+TGG | 0.255837 | 4.2:-24069764 | None:intergenic |
| GTGCAATTGATCAGTAAATT+TGG | 0.302237 | 4.2:+24076936 | MS.gene39590:CDS |
| TTTGTACATACAGGAGTTAA+TGG | 0.317174 | 4.2:+24076894 | MS.gene39590:intron |
| TAATACGATCCTCTTTGATT+TGG | 0.320846 | 4.2:-24078326 | None:intergenic |
| TGTTTGAAGGCCAGAACTTT+AGG | 0.328926 | 4.2:+24074531 | MS.gene39590:intron |
| TACTCCTGATACCATTGTTC+AGG | 0.336956 | 4.2:+24074592 | MS.gene39590:CDS |
| AAGCACTGATAGCTGTTAAA+AGG | 0.346617 | 4.2:-24079307 | None:intergenic |
| GTGCAATCATATCCACAAAC+TGG | 0.353594 | 4.2:-24076158 | None:intergenic |
| CTCAATAGGATGGTTTCGTT+TGG | 0.356859 | 4.2:+24076093 | MS.gene39590:intron |
| CATACAGGAGTTAATGGAAT+TGG | 0.360524 | 4.2:+24076900 | MS.gene39590:intron |
| ATAGATTGTGCCTAAAGTTC+TGG | 0.365868 | 4.2:-24074541 | None:intergenic |
| GAGGACTTTGCAGAGCGATT+TGG | 0.367514 | 4.2:+24076120 | MS.gene39590:CDS |
| TTCATTAAATGATAGGTTCC+AGG | 0.372126 | 4.2:+24074723 | MS.gene39590:intron |
| GACAGATCTGATAATATTCC+AGG | 0.374957 | 4.2:+24076189 | MS.gene39590:CDS |
| AAATTGTTCTAAACAAGAAA+TGG | 0.375688 | 4.2:-24069465 | None:intergenic |
| TCTGCCATTGGCCGCAGCTT+CGG | 0.385305 | 4.2:-24069819 | None:intergenic |
| GTATCATTTGTGGGAAAAGT+TGG | 0.390864 | 4.2:+24079370 | MS.gene39590:CDS |
| GGCAGAGTTATGCTTATTGA+TGG | 0.411530 | 4.2:+24069836 | MS.gene39590:CDS |
| CTAGCACATGCCGATGGAAA+TGG | 0.412460 | 4.2:+24070577 | MS.gene39590:CDS |
| ATGCCGATGGAAATGGAGAT+TGG | 0.416221 | 4.2:+24070584 | MS.gene39590:CDS |
| CACCACGTGGAGCAAGTCTT+AGG | 0.416399 | 4.2:-24075238 | None:intergenic |
| TGTGGGAAAAGTTGGATTCA+AGG | 0.423479 | 4.2:+24079378 | MS.gene39590:CDS |
| CCCAGCATCAACACTCCTTA+AGG | 0.425425 | 4.2:-24074778 | None:intergenic |
| TGATACACTCTCCTGATGAT+AGG | 0.425535 | 4.2:-24079353 | None:intergenic |
| GAATTAAGAGTCTGACGAAA+TGG | 0.427805 | 4.2:-24073391 | None:intergenic |
| AGTCCCTGAACAATGGTATC+AGG | 0.428886 | 4.2:-24074596 | None:intergenic |
| TGATTGGAACATTAGCCTTA+AGG | 0.432237 | 4.2:+24074763 | MS.gene39590:CDS |
| TGGCCTTGATGGATGCTTTG+AGG | 0.438000 | 4.2:-24074625 | None:intergenic |
| GGAGGCACCGTTCCGGTGGT+TGG | 0.438122 | 4.2:-24069743 | None:intergenic |
| AGAGCATACTACAAGCTGTT+AGG | 0.443275 | 4.2:+24069875 | MS.gene39590:CDS |
| GGCTATATTAAGCAAGGAAC+TGG | 0.443781 | 4.2:+24078590 | MS.gene39590:CDS |
| AATTTCTGGAGGCACCGTTC+CGG | 0.448299 | 4.2:-24069750 | None:intergenic |
| ATTGGCCGCAGCTTCGGCAT+TGG | 0.451298 | 4.2:-24069813 | None:intergenic |
| AGGCACATTGGAGAAGTTGT+TGG | 0.456254 | 4.2:+24078293 | MS.gene39590:intron |
| CGTGAAGAACGAAGAAGGTT+TGG | 0.460304 | 4.2:-24069556 | None:intergenic |
| CAGGAGAGTGTATCATTTGT+GGG | 0.465284 | 4.2:+24079361 | MS.gene39590:CDS |
| CTTAACTGAAAATGCTGAGC+AGG | 0.470819 | 4.2:+24078569 | MS.gene39590:CDS |
| TCTGAAACCATCCCAGTTTG+TGG | 0.473588 | 4.2:+24076146 | MS.gene39590:CDS |
| TGCCGATGGAAATGGAGATT+GGG | 0.481773 | 4.2:+24070585 | MS.gene39590:CDS |
| GCCTTAAGGAGTGTTGATGC+TGG | 0.485198 | 4.2:+24074777 | MS.gene39590:CDS |
| GAAATGGGAGTGAAGAAAGA+AGG | 0.487122 | 4.2:-24069449 | None:intergenic |
| GACTTTGCAGAGCGATTTGG+AGG | 0.488400 | 4.2:+24076123 | MS.gene39590:CDS |
| TCAGGAGAGTGTATCATTTG+TGG | 0.491632 | 4.2:+24079360 | MS.gene39590:CDS |
| TCTTTGGATTCACATAAAGA+TGG | 0.492913 | 4.2:+24069701 | MS.gene39590:CDS |
| AATTGTTCTAAACAAGAAAT+GGG | 0.504361 | 4.2:-24069464 | None:intergenic |
| CAAAGAGGATCGTATTAGAA+AGG | 0.504847 | 4.2:+24078332 | MS.gene39590:CDS |
| TTTCAGCTGATCCTATCATC+AGG | 0.505189 | 4.2:+24079342 | MS.gene39590:CDS |
| AATGCCGAAGCTGCGGCCAA+TGG | 0.509054 | 4.2:+24069815 | MS.gene39590:CDS |
| AATTTGATATCTTACCATCA+TGG | 0.513196 | 4.2:-24071610 | None:intergenic |
| GTAGTTTCCGACGGTGGTGG+TGG | 0.520849 | 4.2:-24069515 | None:intergenic |
| GGACGATTGCTCTTGTACGC+AGG | 0.526137 | 4.2:-24074566 | None:intergenic |
| TTCTGGAGGCACCGTTCCGG+TGG | 0.532696 | 4.2:-24069747 | None:intergenic |
| GTTTCCGACGGTGGTGGTGG+TGG | 0.539455 | 4.2:-24069512 | None:intergenic |
| AGAGTAGTTTCCGACGGTGG+TGG | 0.545071 | 4.2:-24069518 | None:intergenic |
| GCTATCAGTGCTTACGCAGA+AGG | 0.547320 | 4.2:+24079317 | MS.gene39590:CDS |
| GTGAATGGTACCATGTAGGA+TGG | 0.550198 | 4.2:-24078763 | None:intergenic |
| GATGGTTTCGTTTGGAGCTG+AGG | 0.553880 | 4.2:+24076101 | MS.gene39590:CDS |
| CCTTAAGGAGTGTTGATGCT+GGG | 0.554132 | 4.2:+24074778 | MS.gene39590:CDS |
| GTGCCTCCAGAAATTAGTTA+CGG | 0.556767 | 4.2:+24069758 | MS.gene39590:CDS |
| TACAGTGAATGGTACCATGT+AGG | 0.557179 | 4.2:-24078767 | None:intergenic |
| GTAGAAGCAGATGATGTGAT+TGG | 0.557576 | 4.2:+24074747 | MS.gene39590:CDS |
| ATTCATTCCTTCCCATGTGG+TGG | 0.559114 | 4.2:+24071414 | MS.gene39590:CDS |
| AATAAGCATAACTCTGCCAT+TGG | 0.562489 | 4.2:-24069831 | None:intergenic |
| ACATCATCTGCTTCTACACC+TGG | 0.562883 | 4.2:-24074741 | None:intergenic |
| ACTCCTGATACCATTGTTCA+GGG | 0.563327 | 4.2:+24074593 | MS.gene39590:CDS |
| TCTGCAACTTCTCCAACCAC+CGG | 0.572776 | 4.2:+24069731 | MS.gene39590:CDS |
| GTGCGCGTGAAGAACGAAGA+AGG | 0.572912 | 4.2:-24069561 | None:intergenic |
| TTGTGATCTCCCATCCTACA+TGG | 0.573874 | 4.2:+24078753 | MS.gene39590:CDS |
| CAAAGCATCCATCAAGGCCA+TGG | 0.574954 | 4.2:+24074628 | MS.gene39590:CDS |
| GACGATTGCTCTTGTACGCA+GGG | 0.578661 | 4.2:-24074565 | None:intergenic |
| ACTTATTTGAATAGGCACAT+TGG | 0.579686 | 4.2:+24078281 | MS.gene39590:intron |
| TTCACGCGCACCACTCTCCA+AGG | 0.580932 | 4.2:+24069573 | MS.gene39590:CDS |
| AAACATACCACCACATGGGA+AGG | 0.581300 | 4.2:-24071421 | None:intergenic |
| GAGTGTTGATGCTGGGTACA+AGG | 0.582786 | 4.2:+24074785 | MS.gene39590:CDS |
| TGCAATCATATCCACAAACT+GGG | 0.587318 | 4.2:-24076157 | None:intergenic |
| AACCCAATCTCCATTTCCAT+CGG | 0.588128 | 4.2:-24070587 | None:intergenic |
| TGAACAATGGTATCAGGAGT+AGG | 0.591744 | 4.2:-24074590 | None:intergenic |
| CAGGTAGTGTTTGACCATGA+TGG | 0.597006 | 4.2:+24071596 | MS.gene39590:intron |
| AACTTCTCCAACCACCGGAA+CGG | 0.599945 | 4.2:+24069736 | MS.gene39590:CDS |
| GGGAGTGAAGAAAGAAGGAG+TGG | 0.600096 | 4.2:-24069444 | None:intergenic |
| GGTAACAGAACCAATGTGCA+CGG | 0.607886 | 4.2:-24069792 | None:intergenic |
| GGAATTCATTCCTTCCCATG+TGG | 0.611125 | 4.2:+24071411 | MS.gene39590:CDS |
| GCTGCTCAACCCGTGCACAT+TGG | 0.615289 | 4.2:+24069782 | MS.gene39590:CDS |
| ATCATATCCACAAACTGGGA+TGG | 0.619113 | 4.2:-24076153 | None:intergenic |
| ATCTGTTGACCAAATCAAAG+AGG | 0.622060 | 4.2:+24078317 | MS.gene39590:CDS |
| GTAGTATGCTCTATGAATCA+CGG | 0.624519 | 4.2:-24069864 | None:intergenic |
| TGTTACCAATGCCGAAGCTG+CGG | 0.627137 | 4.2:+24069808 | MS.gene39590:CDS |
| GGTCATCTAGCACATGCCGA+TGG | 0.627959 | 4.2:+24070571 | MS.gene39590:CDS |
| ATACCTCAAAGCATCCATCA+AGG | 0.628176 | 4.2:+24074622 | MS.gene39590:CDS |
| GGACAAAACATACCACCACA+TGG | 0.628503 | 4.2:-24071426 | None:intergenic |
| TGAATGGTACCATGTAGGAT+GGG | 0.629221 | 4.2:-24078762 | None:intergenic |
| CATGGCCATAAAAGTCATTG+AGG | 0.637462 | 4.2:+24074646 | MS.gene39590:CDS |
| GTAACAGAACCAATGTGCAC+GGG | 0.641689 | 4.2:-24069791 | None:intergenic |
| GTGTACACTGTACAGGACAA+TGG | 0.645776 | 4.2:+24079269 | MS.gene39590:intron |
| TGGTGGTGGTGTGAATGCGA+CGG | 0.646191 | 4.2:-24069498 | None:intergenic |
| TCTCTTGTTTACTAAACCCG+AGG | 0.649424 | 4.2:+24078795 | MS.gene39590:CDS |
| TGAGCAGGCTATATTAAGCA+AGG | 0.650855 | 4.2:+24078584 | MS.gene39590:CDS |
| ACAATGGTATCAGGAGTAGG+AGG | 0.659704 | 4.2:-24074587 | None:intergenic |
| GTATTGAAGTCCCTGAACAA+TGG | 0.660032 | 4.2:-24074603 | None:intergenic |
| CTCCTAAGACTTGCTCCACG+TGG | 0.661892 | 4.2:+24075236 | MS.gene39590:CDS |
| GACAAAACATACCACCACAT+GGG | 0.676208 | 4.2:-24071425 | None:intergenic |
| GCGAGAGTAGTTTCCGACGG+TGG | 0.676946 | 4.2:-24069521 | None:intergenic |
| GCTCCGTAACTAATTTCTGG+AGG | 0.677777 | 4.2:-24069761 | None:intergenic |
| TTGATAGCAAAATTGCACCA+TGG | 0.689907 | 4.2:+24070550 | MS.gene39590:intron |
| GAGTGTGTTACCTTGGAGAG+TGG | 0.691852 | 4.2:-24069583 | None:intergenic |
| CGGCATGTGCTAGATGACCA+TGG | 0.709874 | 4.2:-24070567 | None:intergenic |
| ACTGCGAGAGTAGTTTCCGA+CGG | 0.710307 | 4.2:-24069524 | None:intergenic |
| GTGGTGTGAATGCGACGGAG+AGG | 0.721831 | 4.2:-24069493 | None:intergenic |
| AGTCTTAGGAGACGTAACGA+CGG | 0.740227 | 4.2:-24075224 | None:intergenic |
| GATACTAACTGATCACCACG+TGG | 0.760957 | 4.2:-24075251 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 24069424 | 24079403 | 24069424 | ID=MS.gene39590 |
| chr4.2 | mRNA | 24069424 | 24079403 | 24069424 | ID=MS.gene39590.t1;Parent=MS.gene39590 |
| chr4.2 | exon | 24069424 | 24069594 | 24069424 | ID=MS.gene39590.t1.exon1;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24069424 | 24069594 | 24069424 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24069686 | 24069896 | 24069686 | ID=MS.gene39590.t1.exon2;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24069686 | 24069896 | 24069686 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24070557 | 24070636 | 24070557 | ID=MS.gene39590.t1.exon3;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24070557 | 24070636 | 24070557 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24071397 | 24071435 | 24071397 | ID=MS.gene39590.t1.exon4;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24071397 | 24071435 | 24071397 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24071599 | 24071617 | 24071599 | ID=MS.gene39590.t1.exon5;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24071599 | 24071617 | 24071599 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24073386 | 24073439 | 24073386 | ID=MS.gene39590.t1.exon6;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24073386 | 24073439 | 24073386 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24074540 | 24074667 | 24074540 | ID=MS.gene39590.t1.exon7;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24074540 | 24074667 | 24074540 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24074738 | 24074806 | 24074738 | ID=MS.gene39590.t1.exon8;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24074738 | 24074806 | 24074738 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24075179 | 24075264 | 24075179 | ID=MS.gene39590.t1.exon9;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24075179 | 24075264 | 24075179 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24076101 | 24076210 | 24076101 | ID=MS.gene39590.t1.exon10;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24076101 | 24076210 | 24076101 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24076907 | 24076957 | 24076907 | ID=MS.gene39590.t1.exon11;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24076907 | 24076957 | 24076907 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24078295 | 24078353 | 24078295 | ID=MS.gene39590.t1.exon12;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24078295 | 24078353 | 24078295 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24078567 | 24078611 | 24078567 | ID=MS.gene39590.t1.exon13;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24078567 | 24078611 | 24078567 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24078742 | 24078816 | 24078742 | ID=MS.gene39590.t1.exon14;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24078742 | 24078816 | 24078742 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| chr4.2 | exon | 24079284 | 24079403 | 24079284 | ID=MS.gene39590.t1.exon15;Parent=MS.gene39590.t1 |
| chr4.2 | CDS | 24079284 | 24079403 | 24079284 | ID=cds.MS.gene39590.t1;Parent=MS.gene39590.t1 |
| Gene Sequence |
| Protein sequence |
