Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39716.t1 | XP_014633430.1 | 94.3 | 87 | 5 | 0 | 1 | 87 | 6 | 92 | 2.70E-14 | 87.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39716.t1 | A0A4D6KLM6 | 93.1 | 87 | 6 | 0 | 1 | 87 | 41 | 127 | 4.3e-14 | 86.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050384 | MS.gene39716 | 0.803551 | 3.30E-49 | -1.69E-46 |
| MS.gene050386 | MS.gene39716 | 0.80564 | 1.21E-49 | -1.69E-46 |
| MS.gene051902 | MS.gene39716 | 0.804774 | 1.84E-49 | -1.69E-46 |
| MS.gene052072 | MS.gene39716 | 0.804923 | 1.71E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene39716 | 0.817272 | 3.59E-52 | -1.69E-46 |
| MS.gene058054 | MS.gene39716 | 0.809984 | 1.44E-50 | -1.69E-46 |
| MS.gene058055 | MS.gene39716 | 0.805466 | 1.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGACAAGATGCATGGATTC+GGG | 0.332377 | 8.1:+86001173 | MS.gene39716:CDS |
| TTGACACCAACAGGGGAAAC+TGG | 0.381788 | 8.1:-86001352 | None:intergenic |
| CTGGAGTGATTGACACCAAC+AGG | 0.386537 | 8.1:-86001361 | None:intergenic |
| GCGGACAAGATGCATGGATT+CGG | 0.398614 | 8.1:+86001172 | MS.gene39716:CDS |
| GCAAATGGCCATCGTTATGA+AGG | 0.442940 | 8.1:+86001208 | MS.gene39716:CDS |
| GGAATGTATACATTTAGAAA+TGG | 0.453121 | 8.1:+86001262 | MS.gene39716:CDS |
| GAGAAACTCAATCTGGTCAC+TGG | 0.482465 | 8.1:+86001284 | MS.gene39716:CDS |
| GAGGGGAAAAGACATGGTCT+TGG | 0.489572 | 8.1:+86001241 | MS.gene39716:CDS |
| ACATATCCAGTTTCCCCTGT+TGG | 0.495511 | 8.1:+86001346 | MS.gene39716:CDS |
| TATGTGGCGTTCTGCGTGCT+TGG | 0.500334 | 8.1:-86001328 | None:intergenic |
| AGAAATGGAGAAACTCAATC+TGG | 0.509744 | 8.1:+86001277 | MS.gene39716:CDS |
| CTGCACTGTATTAAGTACTC+TGG | 0.572225 | 8.1:-86001380 | None:intergenic |
| AGAAATGGAGATACGTATGC+TGG | 0.572290 | 8.1:+86001139 | None:intergenic |
| AACAGGGGAAACTGGATATG+TGG | 0.576987 | 8.1:-86001344 | None:intergenic |
| GCCATCGTTATGAAGGAACC+TGG | 0.582273 | 8.1:+86001215 | MS.gene39716:CDS |
| TATGAAGGAACCTGGCATGA+GGG | 0.590379 | 8.1:+86001223 | MS.gene39716:CDS |
| CAGAGTACTTAATACAGTGC+AGG | 0.593895 | 8.1:+86001381 | MS.gene39716:CDS |
| ATGAAGGAACCTGGCATGAG+GGG | 0.602811 | 8.1:+86001224 | MS.gene39716:CDS |
| TGGCATGAGGGGAAAAGACA+TGG | 0.607412 | 8.1:+86001235 | MS.gene39716:CDS |
| TTATGAAGGAACCTGGCATG+AGG | 0.612995 | 8.1:+86001222 | MS.gene39716:CDS |
| TATATTGCGGACAAGATGCA+TGG | 0.616225 | 8.1:+86001166 | MS.gene39716:CDS |
| GCCAGGTTCCTTCATAACGA+TGG | 0.629240 | 8.1:-86001216 | None:intergenic |
| TGGAGTGATTGACACCAACA+GGG | 0.636221 | 8.1:-86001360 | None:intergenic |
| GTATGCTGGTGAGTATATTG+CGG | 0.683410 | 8.1:+86001153 | MS.gene39716:CDS |
| GGACAAGATGCATGGATTCG+GGG | 0.701613 | 8.1:+86001174 | MS.gene39716:CDS |
| GGAGTGATTGACACCAACAG+GGG | 0.733255 | 8.1:-86001359 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GGAATGTATACATTTAGAAA+TGG | + | chr8.1:86001262-86001281 | MS.gene39716:CDS | 25.0% |
| AGAAATGGAGAAACTCAATC+TGG | + | chr8.1:86001277-86001296 | MS.gene39716:CDS | 35.0% | |
| CAATCTGGTCACTGGAAAAA+TGG | + | chr8.1:86001292-86001311 | MS.gene39716:CDS | 40.0% | |
| CTGCACTGTATTAAGTACTC+TGG | - | chr8.1:86001383-86001402 | None:intergenic | 40.0% | |
| GTATGCTGGTGAGTATATTG+CGG | + | chr8.1:86001153-86001172 | MS.gene39716:CDS | 40.0% | |
| TATATTGCGGACAAGATGCA+TGG | + | chr8.1:86001166-86001185 | MS.gene39716:CDS | 40.0% | |
| ! | GGGGTATACTGTTTTGCAAA+TGG | + | chr8.1:86001193-86001212 | MS.gene39716:CDS | 40.0% |
| AACAGGGGAAACTGGATATG+TGG | - | chr8.1:86001347-86001366 | None:intergenic | 45.0% | |
| ACATATCCAGTTTCCCCTGT+TGG | + | chr8.1:86001346-86001365 | MS.gene39716:CDS | 45.0% | |
| GAGAAACTCAATCTGGTCAC+TGG | + | chr8.1:86001284-86001303 | MS.gene39716:CDS | 45.0% | |
| GCAAATGGCCATCGTTATGA+AGG | + | chr8.1:86001208-86001227 | MS.gene39716:CDS | 45.0% | |
| TATGAAGGAACCTGGCATGA+GGG | + | chr8.1:86001223-86001242 | MS.gene39716:CDS | 45.0% | |
| TGGAGTGATTGACACCAACA+GGG | - | chr8.1:86001363-86001382 | None:intergenic | 45.0% | |
| TTATGAAGGAACCTGGCATG+AGG | + | chr8.1:86001222-86001241 | MS.gene39716:CDS | 45.0% | |
| ATGAAGGAACCTGGCATGAG+GGG | + | chr8.1:86001224-86001243 | MS.gene39716:CDS | 50.0% | |
| CGGACAAGATGCATGGATTC+GGG | + | chr8.1:86001173-86001192 | MS.gene39716:CDS | 50.0% | |
| CTGGAGTGATTGACACCAAC+AGG | - | chr8.1:86001364-86001383 | None:intergenic | 50.0% | |
| GAGGGGAAAAGACATGGTCT+TGG | + | chr8.1:86001241-86001260 | MS.gene39716:CDS | 50.0% | |
| GCCAGGTTCCTTCATAACGA+TGG | - | chr8.1:86001219-86001238 | None:intergenic | 50.0% | |
| GCCATCGTTATGAAGGAACC+TGG | + | chr8.1:86001215-86001234 | MS.gene39716:CDS | 50.0% | |
| GCGGACAAGATGCATGGATT+CGG | + | chr8.1:86001172-86001191 | MS.gene39716:CDS | 50.0% | |
| GGACAAGATGCATGGATTCG+GGG | + | chr8.1:86001174-86001193 | MS.gene39716:CDS | 50.0% | |
| GGAGTGATTGACACCAACAG+GGG | - | chr8.1:86001362-86001381 | None:intergenic | 50.0% | |
| TGGCATGAGGGGAAAAGACA+TGG | + | chr8.1:86001235-86001254 | MS.gene39716:CDS | 50.0% | |
| TTGACACCAACAGGGGAAAC+TGG | - | chr8.1:86001355-86001374 | None:intergenic | 50.0% | |
| ! | ATGTCTTTTCCCCTCATGCC+AGG | - | chr8.1:86001236-86001255 | None:intergenic | 50.0% |
| TATGTGGCGTTCTGCGTGCT+TGG | - | chr8.1:86001331-86001350 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 86001141 | 86001402 | 86001141 | ID=MS.gene39716 |
| chr8.1 | mRNA | 86001141 | 86001402 | 86001141 | ID=MS.gene39716.t1;Parent=MS.gene39716 |
| chr8.1 | exon | 86001141 | 86001402 | 86001141 | ID=MS.gene39716.t1.exon1;Parent=MS.gene39716.t1 |
| chr8.1 | CDS | 86001141 | 86001402 | 86001141 | ID=cds.MS.gene39716.t1;Parent=MS.gene39716.t1 |
| Gene Sequence |
| Protein sequence |
