Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39808.t1 | XP_024638986.1 | 97.7 | 177 | 4 | 0 | 1 | 177 | 1 | 177 | 2.40E-94 | 354.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39808.t1 | Q9FJI1 | 67.1 | 167 | 55 | 0 | 7 | 173 | 10 | 176 | 1.1e-63 | 244.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39808.t1 | G7K2Z5 | 97.7 | 177 | 4 | 0 | 1 | 177 | 1 | 177 | 1.7e-94 | 354.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene39808 | 0.826514 | 2.60E-54 | -1.69E-46 |
| MS.gene050303 | MS.gene39808 | 0.816817 | 4.55E-52 | -1.69E-46 |
| MS.gene055512 | MS.gene39808 | 0.811427 | 7.04E-51 | -1.69E-46 |
| MS.gene055555 | MS.gene39808 | 0.800452 | 1.43E-48 | -1.69E-46 |
| MS.gene057705 | MS.gene39808 | 0.836875 | 7.24E-57 | -1.69E-46 |
| MS.gene05954 | MS.gene39808 | 0.81069 | 1.02E-50 | -1.69E-46 |
| MS.gene05978 | MS.gene39808 | 0.806838 | 6.77E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39808.t1 | MTR_5g087910 | 97.740 | 177 | 4 | 0 | 1 | 177 | 1 | 177 | 2.16e-127 | 355 |
| MS.gene39808.t1 | MTR_5g087910 | 97.740 | 177 | 4 | 0 | 1 | 177 | 73 | 249 | 3.23e-126 | 355 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39808.t1 | AT5G60340 | 67.066 | 167 | 55 | 0 | 7 | 173 | 10 | 176 | 2.94e-83 | 243 |
Find 38 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAAGGAAACCAAACATTT+TGG | 0.273161 | 5.4:+69192750 | MS.gene39808:CDS |
| CCACATCAATATTGGAGATT+TGG | 0.293429 | 5.4:+69192840 | MS.gene39808:CDS |
| TTACATTCTTAATGAAGATT+TGG | 0.302756 | 5.4:+69192909 | MS.gene39808:CDS |
| CAGCTCAACCACATCAATAT+TGG | 0.350514 | 5.4:+69192832 | MS.gene39808:CDS |
| GCTCAGACGGTCATACAAAA+TGG | 0.376389 | 5.4:-69193371 | None:intergenic |
| GATTTCACATTCGACGTTGT+TGG | 0.388413 | 5.4:-69193518 | None:intergenic |
| GTACCAGTCACCAAAATGTT+TGG | 0.424577 | 5.4:-69192760 | None:intergenic |
| TGATGAACTTGAGGATGTTA+TGG | 0.431142 | 5.4:+69193258 | MS.gene39808:CDS |
| GTGGTTGTCTTTCCTGTCCC+TGG | 0.431186 | 5.4:-69192784 | None:intergenic |
| GAATTGGCCCATCCCAGTAT+AGG | 0.434368 | 5.4:+69193660 | MS.gene39808:CDS |
| TGACAGATTGGATCAGGAAT+TGG | 0.437273 | 5.4:+69193644 | MS.gene39808:CDS |
| AATTGGCCCATCCCAGTATA+GGG | 0.447984 | 5.4:+69193661 | MS.gene39808:CDS |
| AGAAGAACTTGCATGATGGC+TGG | 0.459493 | 5.4:+69192869 | MS.gene39808:CDS |
| CAACTCTGACAGATTGGATC+AGG | 0.470384 | 5.4:+69193638 | MS.gene39808:CDS |
| GGGAATATTGTGGACTATCA+TGG | 0.474310 | 5.4:+69193289 | MS.gene39808:CDS |
| GTGATTTCTTTCCCGAGCGA+TGG | 0.476262 | 5.4:+69193314 | MS.gene39808:CDS |
| ACTTGAGGATGTTATGGAAG+AGG | 0.497760 | 5.4:+69193264 | MS.gene39808:CDS |
| CGAGCGATGGTTTGATTGTG+TGG | 0.514486 | 5.4:+69193327 | MS.gene39808:CDS |
| ATGTTGCAACTCTGACAGAT+TGG | 0.522799 | 5.4:+69193632 | MS.gene39808:CDS |
| CTTGAGGATGTTATGGAAGA+GGG | 0.525425 | 5.4:+69193265 | MS.gene39808:CDS |
| CACACAATCAAACCATCGCT+CGG | 0.528082 | 5.4:-69193326 | None:intergenic |
| GGAAGAGGGAGGGAATATTG+TGG | 0.540361 | 5.4:+69193279 | MS.gene39808:CDS |
| AAAATACCCTATACTGGGAT+GGG | 0.545741 | 5.4:-69193667 | None:intergenic |
| GAGGATGTTATGGAAGAGGG+AGG | 0.559657 | 5.4:+69193268 | MS.gene39808:CDS |
| TGTATGACCGTCTGAGCAGA+AGG | 0.560928 | 5.4:+69193377 | MS.gene39808:CDS |
| ACACAATCAAACCATCGCTC+GGG | 0.566646 | 5.4:-69193325 | None:intergenic |
| GTGACTGGTACACCAGGGAC+AGG | 0.576936 | 5.4:+69192772 | MS.gene39808:CDS |
| GTAGGTGTGTGATGAACTTG+AGG | 0.579640 | 5.4:+69193249 | MS.gene39808:intron |
| GAAGAACTTGCATGATGGCT+GGG | 0.584441 | 5.4:+69192870 | MS.gene39808:CDS |
| AAAGAGAAGAACTTGCATGA+TGG | 0.601786 | 5.4:+69192865 | MS.gene39808:CDS |
| TGACATACCTTCTGCTCAGA+CGG | 0.610039 | 5.4:-69193384 | None:intergenic |
| AGGATGTTATGGAAGAGGGA+GGG | 0.612954 | 5.4:+69193269 | MS.gene39808:CDS |
| TGAAAGACAGTATCAAGAGA+AGG | 0.619884 | 5.4:+69192734 | MS.gene39808:CDS |
| TTCAGCTAGAGCAGTTGACG+TGG | 0.628337 | 5.4:-69192803 | None:intergenic |
| CCAAATCTCCAATATTGATG+TGG | 0.645106 | 5.4:-69192840 | None:intergenic |
| GGCTAAAGAAAGTTACGCGG+AGG | 0.677847 | 5.4:+69193561 | MS.gene39808:CDS |
| ATTGATGTGGTTGAGCTGAG+TGG | 0.681056 | 5.4:-69192827 | None:intergenic |
| GGAGGCTAAAGAAAGTTACG+CGG | 0.771874 | 5.4:+69193558 | MS.gene39808:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATATAAAGATTAACAAA+TGG | - | chr5.4:69193201-69193220 | None:intergenic | 10.0% |
| !! | TTTGTTAATCTTTATATATT+AGG | + | chr5.4:69193201-69193220 | MS.gene39808:intron | 10.0% |
| !! | TTACATTCTTAATGAAGATT+TGG | + | chr5.4:69192909-69192928 | MS.gene39808:CDS | 20.0% |
| !!! | ATAATGACTCTTCAATATTT+TGG | + | chr5.4:69193124-69193143 | MS.gene39808:intron | 20.0% |
| ! | CAAACAAAACAATACAAGAA+CGG | - | chr5.4:69192961-69192980 | None:intergenic | 25.0% |
| ! | GCAATTCTATTGTATATTTC+AGG | + | chr5.4:69193438-69193457 | MS.gene39808:intron | 25.0% |
| ! | TAAGCTACCAATTTATATAC+TGG | + | chr5.4:69192932-69192951 | MS.gene39808:intron | 25.0% |
| ! | TATCAATAACAGTGAAATGA+GGG | - | chr5.4:69193107-69193126 | None:intergenic | 25.0% |
| ! | TTATCAATAACAGTGAAATG+AGG | - | chr5.4:69193108-69193127 | None:intergenic | 25.0% |
| !! | AGGTTTATGTGTATATCTTT+TGG | + | chr5.4:69193221-69193240 | MS.gene39808:intron | 25.0% |
| !!! | GTATATCTTTTGGTTTTTGT+AGG | + | chr5.4:69193231-69193250 | MS.gene39808:intron | 25.0% |
| TTGGCATACTATTTACTGAA+TGG | + | chr5.4:69193143-69193162 | MS.gene39808:intron | 30.0% | |
| ! | CAAATGGTCTTTTATCACAA+CGG | - | chr5.4:69193185-69193204 | None:intergenic | 30.0% |
| !! | CGTTCTTGTATTGTTTTGTT+TGG | + | chr5.4:69192959-69192978 | MS.gene39808:intron | 30.0% |
| !!! | AATCTTTCAAGTTTTGCTTG+AGG | + | chr5.4:69193537-69193556 | MS.gene39808:CDS | 30.0% |
| AAAGAGAAGAACTTGCATGA+TGG | + | chr5.4:69192865-69192884 | MS.gene39808:CDS | 35.0% | |
| ACAGGAGTAACATGACTATT+GGG | - | chr5.4:69193049-69193068 | None:intergenic | 35.0% | |
| ACGGTCTCCAGTATATAAAT+TGG | - | chr5.4:69192942-69192961 | None:intergenic | 35.0% | |
| ATAACAGTGAAATGAGGGAA+TGG | - | chr5.4:69193102-69193121 | None:intergenic | 35.0% | |
| CCAAATCTCCAATATTGATG+TGG | - | chr5.4:69192843-69192862 | None:intergenic | 35.0% | |
| CCACATCAATATTGGAGATT+TGG | + | chr5.4:69192840-69192859 | MS.gene39808:CDS | 35.0% | |
| GCAAAAGGACTAAACATTAC+AGG | - | chr5.4:69193067-69193086 | None:intergenic | 35.0% | |
| TACAGGAGTAACATGACTAT+TGG | - | chr5.4:69193050-69193069 | None:intergenic | 35.0% | |
| TGAAAGACAGTATCAAGAGA+AGG | + | chr5.4:69192734-69192753 | MS.gene39808:CDS | 35.0% | |
| TGATGAACTTGAGGATGTTA+TGG | + | chr5.4:69193258-69193277 | MS.gene39808:CDS | 35.0% | |
| ! | GAGAAGGAAACCAAACATTT+TGG | + | chr5.4:69192750-69192769 | MS.gene39808:CDS | 35.0% |
| !! | AAACCAAACATTTTGGTGAC+TGG | + | chr5.4:69192757-69192776 | MS.gene39808:CDS | 35.0% |
| AATGAGGGAATGGTTAAGGA+TGG | - | chr5.4:69193092-69193111 | None:intergenic | 40.0% | |
| ACTTGAGGATGTTATGGAAG+AGG | + | chr5.4:69193264-69193283 | MS.gene39808:CDS | 40.0% | |
| AGGAGTAACATGACTATTGG+GGG | - | chr5.4:69193047-69193066 | None:intergenic | 40.0% | |
| ATGTTGCAACTCTGACAGAT+TGG | + | chr5.4:69193632-69193651 | MS.gene39808:CDS | 40.0% | |
| CAGCTCAACCACATCAATAT+TGG | + | chr5.4:69192832-69192851 | MS.gene39808:CDS | 40.0% | |
| CAGGAGTAACATGACTATTG+GGG | - | chr5.4:69193048-69193067 | None:intergenic | 40.0% | |
| CTTGAGGATGTTATGGAAGA+GGG | + | chr5.4:69193265-69193284 | MS.gene39808:CDS | 40.0% | |
| GATTTCACATTCGACGTTGT+TGG | - | chr5.4:69193521-69193540 | None:intergenic | 40.0% | |
| GTACCAGTCACCAAAATGTT+TGG | - | chr5.4:69192763-69192782 | None:intergenic | 40.0% | |
| GTGAAATGAGGGAATGGTTA+AGG | - | chr5.4:69193096-69193115 | None:intergenic | 40.0% | |
| TGACAGATTGGATCAGGAAT+TGG | + | chr5.4:69193644-69193663 | MS.gene39808:CDS | 40.0% | |
| TGGTTAAGGATGGATGCAAA+AGG | - | chr5.4:69193082-69193101 | None:intergenic | 40.0% | |
| TTCTTCATCTCTGTGACAGA+GGG | + | chr5.4:69193476-69193495 | MS.gene39808:intron | 40.0% | |
| TTTCTTCATCTCTGTGACAG+AGG | + | chr5.4:69193475-69193494 | MS.gene39808:intron | 40.0% | |
| ! | GGGAATATTGTGGACTATCA+TGG | + | chr5.4:69193289-69193308 | MS.gene39808:CDS | 40.0% |
| !!! | CTTTCAAGTTTTGCTTGAGG+AGG | + | chr5.4:69193540-69193559 | MS.gene39808:CDS | 40.0% |
| ACACAATCAAACCATCGCTC+GGG | - | chr5.4:69193328-69193347 | None:intergenic | 45.0% | |
| AGAAGAACTTGCATGATGGC+TGG | + | chr5.4:69192869-69192888 | MS.gene39808:CDS | 45.0% | |
| AGGATGTTATGGAAGAGGGA+GGG | + | chr5.4:69193269-69193288 | MS.gene39808:CDS | 45.0% | |
| ATTGATGTGGTTGAGCTGAG+TGG | - | chr5.4:69192830-69192849 | None:intergenic | 45.0% | |
| CAACTCTGACAGATTGGATC+AGG | + | chr5.4:69193638-69193657 | MS.gene39808:CDS | 45.0% | |
| CACACAATCAAACCATCGCT+CGG | - | chr5.4:69193329-69193348 | None:intergenic | 45.0% | |
| GAAGAACTTGCATGATGGCT+GGG | + | chr5.4:69192870-69192889 | MS.gene39808:CDS | 45.0% | |
| GCTCAGACGGTCATACAAAA+TGG | - | chr5.4:69193374-69193393 | None:intergenic | 45.0% | |
| GGAGGCTAAAGAAAGTTACG+CGG | + | chr5.4:69193558-69193577 | MS.gene39808:CDS | 45.0% | |
| GTAGGTGTGTGATGAACTTG+AGG | + | chr5.4:69193249-69193268 | MS.gene39808:intron | 45.0% | |
| TGACATACCTTCTGCTCAGA+CGG | - | chr5.4:69193387-69193406 | None:intergenic | 45.0% | |
| !! | ATTTTGGTGACTGGTACACC+AGG | + | chr5.4:69192766-69192785 | MS.gene39808:CDS | 45.0% |
| !! | GGTGTTGTTAGCGATGCATT+AGG | + | chr5.4:69192980-69192999 | MS.gene39808:intron | 45.0% |
| !! | TTTTGGTGACTGGTACACCA+GGG | + | chr5.4:69192767-69192786 | MS.gene39808:CDS | 45.0% |
| ACATGACTATTGGGGGCTAG+TGG | - | chr5.4:69193040-69193059 | None:intergenic | 50.0% | |
| CGAGCGATGGTTTGATTGTG+TGG | + | chr5.4:69193327-69193346 | MS.gene39808:CDS | 50.0% | |
| GAGGATGTTATGGAAGAGGG+AGG | + | chr5.4:69193268-69193287 | MS.gene39808:CDS | 50.0% | |
| GGAAGAGGGAGGGAATATTG+TGG | + | chr5.4:69193279-69193298 | MS.gene39808:CDS | 50.0% | |
| GGCTAAAGAAAGTTACGCGG+AGG | + | chr5.4:69193561-69193580 | MS.gene39808:CDS | 50.0% | |
| GTGATTTCTTTCCCGAGCGA+TGG | + | chr5.4:69193314-69193333 | MS.gene39808:CDS | 50.0% | |
| TGTATGACCGTCTGAGCAGA+AGG | + | chr5.4:69193377-69193396 | MS.gene39808:CDS | 50.0% | |
| TTCAGCTAGAGCAGTTGACG+TGG | - | chr5.4:69192806-69192825 | None:intergenic | 50.0% | |
| GTGGTTGTCTTTCCTGTCCC+TGG | - | chr5.4:69192787-69192806 | None:intergenic | 55.0% | |
| GTGACTGGTACACCAGGGAC+AGG | + | chr5.4:69192772-69192791 | MS.gene39808:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 69192730 | 69193681 | 69192730 | ID=MS.gene39808 |
| chr5.4 | mRNA | 69192730 | 69193681 | 69192730 | ID=MS.gene39808.t1;Parent=MS.gene39808 |
| chr5.4 | exon | 69192730 | 69192930 | 69192730 | ID=MS.gene39808.t1.exon1;Parent=MS.gene39808.t1 |
| chr5.4 | CDS | 69192730 | 69192930 | 69192730 | ID=cds.MS.gene39808.t1;Parent=MS.gene39808.t1 |
| chr5.4 | exon | 69193253 | 69193398 | 69193253 | ID=MS.gene39808.t1.exon2;Parent=MS.gene39808.t1 |
| chr5.4 | CDS | 69193253 | 69193398 | 69193253 | ID=cds.MS.gene39808.t1;Parent=MS.gene39808.t1 |
| chr5.4 | exon | 69193495 | 69193681 | 69193495 | ID=MS.gene39808.t1.exon3;Parent=MS.gene39808.t1 |
| chr5.4 | CDS | 69193495 | 69193681 | 69193495 | ID=cds.MS.gene39808.t1;Parent=MS.gene39808.t1 |
| Gene Sequence |
| Protein sequence |
