Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40000.t1 | RHN80197.1 | 95.8 | 432 | 17 | 1 | 3 | 433 | 655 | 1086 | 4.20E-233 | 817 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40000.t1 | Q9FSH9 | 41.0 | 422 | 210 | 12 | 26 | 426 | 42 | 445 | 5.8e-74 | 279.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40000.t1 | G7IEE7 | 95.8 | 432 | 17 | 1 | 3 | 433 | 4 | 435 | 3.0e-233 | 817.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050926 | MS.gene40000 | 0.819884 | 9.18E-53 | -1.69E-46 |
| MS.gene051525 | MS.gene40000 | -0.813617 | 2.34E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene40000 | 0.82032 | 7.30E-53 | -1.69E-46 |
| MS.gene054435 | MS.gene40000 | 0.818917 | 1.53E-52 | -1.69E-46 |
| MS.gene054437 | MS.gene40000 | 0.814797 | 1.28E-51 | -1.69E-46 |
| MS.gene054447 | MS.gene40000 | 0.815575 | 8.62E-52 | -1.69E-46 |
| MS.gene057289 | MS.gene40000 | -0.804173 | 2.45E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene40000 | -0.801475 | 8.85E-49 | -1.69E-46 |
| MS.gene058444 | MS.gene40000 | -0.802492 | 5.47E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene40000 | -0.802591 | 5.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40000.t1 | MTR_1g072420 | 95.833 | 432 | 17 | 1 | 3 | 433 | 4 | 435 | 0.0 | 842 |
| MS.gene40000.t1 | MTR_1g072450 | 88.631 | 431 | 47 | 2 | 4 | 433 | 5 | 434 | 0.0 | 768 |
| MS.gene40000.t1 | MTR_1g072380 | 79.903 | 413 | 74 | 3 | 29 | 433 | 40 | 451 | 0.0 | 656 |
| MS.gene40000.t1 | MTR_2g022450 | 55.012 | 429 | 182 | 7 | 10 | 432 | 6 | 429 | 3.17e-161 | 461 |
| MS.gene40000.t1 | MTR_1g093100 | 52.539 | 453 | 178 | 9 | 1 | 433 | 1 | 436 | 5.59e-155 | 446 |
| MS.gene40000.t1 | MTR_4g117090 | 46.809 | 423 | 198 | 9 | 28 | 427 | 33 | 451 | 3.21e-117 | 350 |
| MS.gene40000.t1 | MTR_2g022430 | 42.760 | 442 | 214 | 11 | 1 | 425 | 1 | 420 | 3.33e-99 | 303 |
| MS.gene40000.t1 | MTR_7g113790 | 40.766 | 444 | 224 | 14 | 8 | 431 | 11 | 435 | 1.39e-98 | 302 |
| MS.gene40000.t1 | MTR_2g022380 | 42.986 | 442 | 219 | 9 | 1 | 428 | 1 | 423 | 2.10e-95 | 294 |
| MS.gene40000.t1 | MTR_2g022370 | 42.694 | 438 | 222 | 9 | 1 | 428 | 1 | 419 | 2.63e-95 | 293 |
| MS.gene40000.t1 | MTR_2g022350 | 42.630 | 441 | 216 | 10 | 1 | 425 | 1 | 420 | 2.82e-93 | 288 |
| MS.gene40000.t1 | MTR_2g022320 | 42.651 | 415 | 197 | 9 | 32 | 426 | 24 | 417 | 1.09e-90 | 281 |
| MS.gene40000.t1 | MTR_2g022460 | 41.262 | 412 | 205 | 11 | 31 | 431 | 26 | 411 | 1.10e-90 | 281 |
| MS.gene40000.t1 | MTR_2g022490 | 41.500 | 400 | 190 | 9 | 28 | 405 | 27 | 404 | 1.37e-87 | 273 |
| MS.gene40000.t1 | MTR_2g022480 | 39.568 | 417 | 222 | 10 | 5 | 405 | 8 | 410 | 3.73e-86 | 270 |
| MS.gene40000.t1 | MTR_0252s0030 | 43.519 | 216 | 102 | 4 | 190 | 394 | 140 | 346 | 2.68e-45 | 160 |
| MS.gene40000.t1 | MTR_1g093130 | 41.212 | 165 | 78 | 4 | 230 | 393 | 6 | 152 | 4.92e-28 | 109 |
| MS.gene40000.t1 | MTR_7g062360 | 23.333 | 390 | 238 | 15 | 54 | 416 | 68 | 423 | 3.02e-20 | 92.8 |
| MS.gene40000.t1 | MTR_7g105850 | 26.034 | 411 | 220 | 19 | 46 | 413 | 91 | 460 | 2.31e-18 | 87.4 |
| MS.gene40000.t1 | MTR_3g046300 | 24.227 | 388 | 232 | 14 | 54 | 416 | 106 | 456 | 9.43e-18 | 85.5 |
| MS.gene40000.t1 | MTR_5g091130 | 22.997 | 387 | 239 | 15 | 54 | 416 | 200 | 551 | 7.87e-16 | 80.1 |
| MS.gene40000.t1 | MTR_3g097150 | 23.907 | 389 | 235 | 17 | 41 | 413 | 98 | 441 | 4.63e-15 | 77.4 |
| MS.gene40000.t1 | MTR_2g022330 | 63.462 | 52 | 19 | 0 | 376 | 427 | 34 | 85 | 5.89e-14 | 67.4 |
| MS.gene40000.t1 | MTR_3g068000 | 23.155 | 393 | 227 | 18 | 58 | 414 | 84 | 437 | 6.74e-14 | 73.6 |
| MS.gene40000.t1 | MTR_8g103880 | 24.422 | 389 | 216 | 18 | 43 | 409 | 114 | 446 | 6.25e-13 | 70.9 |
| MS.gene40000.t1 | MTR_1g111350 | 24.574 | 411 | 232 | 17 | 25 | 414 | 110 | 463 | 2.22e-12 | 68.9 |
| MS.gene40000.t1 | MTR_3g028120 | 23.622 | 381 | 223 | 17 | 54 | 413 | 98 | 431 | 3.81e-12 | 68.2 |
| MS.gene40000.t1 | MTR_4g095270 | 23.117 | 385 | 216 | 16 | 54 | 413 | 89 | 418 | 2.66e-11 | 65.9 |
| MS.gene40000.t1 | MTR_4g007740 | 24.533 | 375 | 219 | 15 | 59 | 414 | 144 | 473 | 2.76e-11 | 65.5 |
| MS.gene40000.t1 | MTR_4g095270 | 23.117 | 385 | 216 | 16 | 54 | 413 | 89 | 418 | 2.78e-11 | 65.9 |
| MS.gene40000.t1 | MTR_5g016260 | 21.939 | 392 | 228 | 15 | 46 | 413 | 89 | 426 | 8.77e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40000.t1 | AT1G03220 | 64.486 | 428 | 145 | 5 | 10 | 433 | 9 | 433 | 0.0 | 547 |
| MS.gene40000.t1 | AT1G03230 | 62.410 | 415 | 149 | 5 | 23 | 433 | 23 | 434 | 0.0 | 515 |
| MS.gene40000.t1 | AT5G19120 | 35.835 | 413 | 208 | 11 | 12 | 413 | 9 | 375 | 7.58e-59 | 197 |
| MS.gene40000.t1 | AT5G19110 | 31.221 | 426 | 257 | 8 | 9 | 427 | 5 | 401 | 2.22e-55 | 189 |
| MS.gene40000.t1 | AT5G19110 | 31.221 | 426 | 257 | 8 | 9 | 427 | 41 | 437 | 4.62e-55 | 189 |
| MS.gene40000.t1 | AT5G48430 | 32.553 | 427 | 244 | 15 | 13 | 428 | 10 | 403 | 1.83e-51 | 179 |
| MS.gene40000.t1 | AT5G19100 | 35.200 | 375 | 184 | 13 | 62 | 425 | 58 | 384 | 5.60e-48 | 169 |
| MS.gene40000.t1 | AT2G39710 | 26.087 | 391 | 212 | 19 | 59 | 414 | 78 | 426 | 1.68e-20 | 93.6 |
| MS.gene40000.t1 | AT3G25700 | 25.060 | 419 | 230 | 18 | 35 | 414 | 72 | 445 | 1.72e-19 | 90.9 |
| MS.gene40000.t1 | AT5G02190 | 21.995 | 391 | 232 | 15 | 59 | 414 | 86 | 438 | 3.03e-14 | 74.7 |
| MS.gene40000.t1 | AT3G52500 | 21.622 | 407 | 248 | 15 | 46 | 414 | 90 | 463 | 2.20e-13 | 72.4 |
| MS.gene40000.t1 | AT3G12700 | 23.155 | 393 | 242 | 15 | 43 | 414 | 103 | 456 | 4.47e-12 | 68.2 |
| MS.gene40000.t1 | AT3G02740 | 22.308 | 390 | 243 | 13 | 46 | 414 | 10 | 360 | 3.96e-11 | 65.1 |
| MS.gene40000.t1 | AT1G25510 | 25.899 | 417 | 232 | 18 | 18 | 415 | 122 | 480 | 7.24e-11 | 64.3 |
Find 111 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCTCTCAACAATCTTTC+AGG | 0.152886 | 1.4:-59356065 | MS.gene40000:CDS |
| TGATTTGGCTGCTTCTAAAT+TGG | 0.163227 | 1.4:-59354915 | MS.gene40000:CDS |
| CACAATCAACCCATAGAAAT+TGG | 0.188301 | 1.4:+59355926 | None:intergenic |
| TTGAGATGGAAGAGCAATTT+TGG | 0.214100 | 1.4:+59355576 | None:intergenic |
| GCACCAACTTTCTTACTAAA+AGG | 0.223724 | 1.4:-59355640 | MS.gene40000:CDS |
| ATCTCGGTGGCCAATTTCTA+TGG | 0.242839 | 1.4:-59355936 | MS.gene40000:CDS |
| GTGGGACTAGTGGGGTTCTT+TGG | 0.257108 | 1.4:+59355974 | None:intergenic |
| TGTAATGAAGTATTGAAAAT+TGG | 0.259063 | 1.4:+59356119 | None:intergenic |
| GGATTTAGCTCCTTGCTCTT+TGG | 0.298601 | 1.4:-59354893 | MS.gene40000:CDS |
| AATGTGATTTGGAGGATATT+TGG | 0.314726 | 1.4:-59355061 | MS.gene40000:CDS |
| TTATCAATCCAATCCTCTAA+TGG | 0.328408 | 1.4:-59355712 | MS.gene40000:CDS |
| GCTTCTGGCATGGCTGGTCT+TGG | 0.335616 | 1.4:-59355604 | MS.gene40000:CDS |
| GGACTTGCAAGTGGTGCTTC+TGG | 0.358517 | 1.4:-59355619 | MS.gene40000:CDS |
| TAATCTTGTGCAATTTGATT+TGG | 0.358886 | 1.4:-59354930 | MS.gene40000:CDS |
| GGTTGATTTGAGCTTTGTAT+TGG | 0.360990 | 1.4:+59355995 | None:intergenic |
| ACTGGGAGAACTAGGGCTTT+TGG | 0.362155 | 1.4:+59356042 | None:intergenic |
| GATTTGGCTGCTTCTAAATT+GGG | 0.364199 | 1.4:-59354914 | MS.gene40000:CDS |
| TCCTGGGTTGAAGCCATTAG+AGG | 0.369624 | 1.4:+59355699 | None:intergenic |
| ATCACCACAGCCGTCGGAAT+TGG | 0.371692 | 1.4:+59355840 | None:intergenic |
| AGTGGTGCTTCTGGCATGGC+TGG | 0.372982 | 1.4:-59355610 | MS.gene40000:CDS |
| TTGTGTCTTGGATTTGTGAA+TGG | 0.380483 | 1.4:-59355004 | MS.gene40000:CDS |
| AAAGCAGAAGCTGTGCTAAC+AGG | 0.404309 | 1.4:+59355403 | None:intergenic |
| CGGAATTGGCGAGGGAGCAT+TGG | 0.411697 | 1.4:+59355854 | None:intergenic |
| CAGGTGTTGTTGTTGCAGCC+AGG | 0.418057 | 1.4:+59355799 | None:intergenic |
| ACAAGGACTTCAATTGTGAT+TGG | 0.421714 | 1.4:-59354971 | MS.gene40000:CDS |
| AAACATAAAGAGGGTGGATT+CGG | 0.425811 | 1.4:-59355170 | MS.gene40000:CDS |
| TCTTCCATCTCAACTAGCTT+CGG | 0.431750 | 1.4:-59355566 | MS.gene40000:CDS |
| GGAATTGGCGAGGGAGCATT+GGG | 0.432376 | 1.4:+59355855 | None:intergenic |
| TATTTGTCTTTCCTCTTCAA+AGG | 0.433460 | 1.4:-59355518 | MS.gene40000:CDS |
| GCTGGTGGCGGTGTGGGTGA+TGG | 0.441452 | 1.4:+59355756 | None:intergenic |
| TGGGCTGAGCGACACCGTGC+AGG | 0.443405 | 1.4:+59355874 | None:intergenic |
| TGTGGGTGATGGAATTGTCC+GGG | 0.446715 | 1.4:+59355767 | None:intergenic |
| GATATTTGGTGCAAATTCAA+TGG | 0.448768 | 1.4:-59355047 | MS.gene40000:CDS |
| TTGTTGTTGCAGCCAGGCTT+TGG | 0.449409 | 1.4:+59355805 | None:intergenic |
| GGTTGAAGCCATTAGAGGAT+TGG | 0.451183 | 1.4:+59355704 | None:intergenic |
| GTGTGGGTGATGGAATTGTC+CGG | 0.454258 | 1.4:+59355766 | None:intergenic |
| CTCGCCAATTCCGACGGCTG+TGG | 0.458653 | 1.4:-59355844 | MS.gene40000:CDS |
| GAAGCTTCTAAAACGGTGTA+AGG | 0.459508 | 1.4:+59355241 | None:intergenic |
| GAAACTGCGACATTTCGTCC+TGG | 0.459544 | 1.4:+59355682 | None:intergenic |
| GTCTGAATCAAGAACAATGT+TGG | 0.467879 | 1.4:+59355456 | None:intergenic |
| AGCAAGTTCACCGCTGGTGG+CGG | 0.469786 | 1.4:+59355744 | None:intergenic |
| TGCAGAACAATGTGATTTGG+AGG | 0.476266 | 1.4:-59355069 | MS.gene40000:CDS |
| ATTTGTCTTTCCTCTTCAAA+GGG | 0.478971 | 1.4:-59355517 | MS.gene40000:CDS |
| TCTGAATCAAGAACAATGTT+GGG | 0.479866 | 1.4:+59355457 | None:intergenic |
| TTCACCGCTGGTGGCGGTGT+GGG | 0.483515 | 1.4:+59355750 | None:intergenic |
| TTATTCGGGGATGGACCTTA+CGG | 0.487213 | 1.4:-59355487 | MS.gene40000:CDS |
| GTAAATAGAAGCTTCTAAAA+CGG | 0.490344 | 1.4:+59355234 | None:intergenic |
| TGTTTCTCATCACCAAAGCC+TGG | 0.494972 | 1.4:-59355817 | MS.gene40000:CDS |
| TCCTCTAATGGCTTCAACCC+AGG | 0.495018 | 1.4:-59355700 | MS.gene40000:CDS |
| AAACTGCGACATTTCGTCCT+GGG | 0.498880 | 1.4:+59355683 | None:intergenic |
| AAGTTGGTGCACATGAGAAT+AGG | 0.499306 | 1.4:+59355653 | None:intergenic |
| TGCAAGTGGTGCTTCTGGCA+TGG | 0.502670 | 1.4:-59355614 | MS.gene40000:CDS |
| TGTCTTGGATTTGTGAATGG+TGG | 0.517202 | 1.4:-59355001 | MS.gene40000:CDS |
| CTGAACGTCATCGTCGATCT+CGG | 0.517558 | 1.4:-59355952 | MS.gene40000:CDS |
| ACACCGTGCAGGGCGGTATG+TGG | 0.520199 | 1.4:+59355885 | None:intergenic |
| GATTGGAGGGTATCAGTTGG+AGG | 0.520806 | 1.4:-59354954 | MS.gene40000:CDS |
| TTAGTGTAACAAAATTCAAA+AGG | 0.525717 | 1.4:+59355142 | None:intergenic |
| TCTCGGTGGCCAATTTCTAT+GGG | 0.528725 | 1.4:-59355935 | MS.gene40000:CDS |
| GAAAGATTGTTGAGAGAAAG+TGG | 0.530349 | 1.4:+59356068 | None:intergenic |
| AATGTGACTGGGACACGATT+GGG | 0.533667 | 1.4:-59355121 | MS.gene40000:CDS |
| TAATGTGACTGGGACACGAT+TGG | 0.534463 | 1.4:-59355122 | MS.gene40000:CDS |
| GTTCTCCCAGTCACAAAAGA+TGG | 0.534725 | 1.4:-59356030 | MS.gene40000:CDS |
| TGACGTTCAGTGGGACTAGT+GGG | 0.536843 | 1.4:+59355965 | None:intergenic |
| ATAGACAACAACGGTGTTGG+TGG | 0.539185 | 1.4:-59355286 | MS.gene40000:CDS |
| ATTGTCCGGGGTGACACCAC+AGG | 0.542125 | 1.4:+59355780 | None:intergenic |
| TCTATAGACAACAACGGTGT+TGG | 0.544980 | 1.4:-59355289 | MS.gene40000:CDS |
| AGGACTTCAATTGTGATTGG+AGG | 0.545988 | 1.4:-59354968 | MS.gene40000:CDS |
| GGGCTGAGCGACACCGTGCA+GGG | 0.548753 | 1.4:+59355875 | None:intergenic |
| ATCTTCAGCAAGTTCACCGC+TGG | 0.549080 | 1.4:+59355738 | None:intergenic |
| TGCAAAATAAGCTCAATAGT+AGG | 0.553873 | 1.4:+59355088 | None:intergenic |
| GTATTGAAAATTGGAAGCCA+TGG | 0.560507 | 1.4:+59356128 | None:intergenic |
| TTCAGCAAGTTCACCGCTGG+TGG | 0.561694 | 1.4:+59355741 | None:intergenic |
| GTTCACCGCTGGTGGCGGTG+TGG | 0.562370 | 1.4:+59355749 | None:intergenic |
| GGACTTCAATTGTGATTGGA+GGG | 0.563151 | 1.4:-59354967 | MS.gene40000:CDS |
| AAAGCCGAAGCTAGTTGAGA+TGG | 0.563385 | 1.4:+59355562 | None:intergenic |
| TGAATGGTGGTAAGAATACA+AGG | 0.567635 | 1.4:-59354988 | MS.gene40000:CDS |
| AATGGTGAATATAAATGATG+AGG | 0.569327 | 1.4:-59355029 | MS.gene40000:CDS |
| ACAGCTTCTGCTTTCTCACA+AGG | 0.569954 | 1.4:-59355394 | MS.gene40000:CDS |
| AGATCGACGATGACGTTCAG+TGG | 0.573829 | 1.4:+59355955 | None:intergenic |
| TAAAACAACTCCCTTTGAAG+AGG | 0.576429 | 1.4:+59355507 | None:intergenic |
| CTGCTGCAAGAAACATAAAG+AGG | 0.579468 | 1.4:-59355180 | MS.gene40000:CDS |
| TGCTCCCTCGCCAATTCCGA+CGG | 0.580900 | 1.4:-59355850 | MS.gene40000:CDS |
| TGTGATTGGAGGGTATCAGT+TGG | 0.585357 | 1.4:-59354957 | MS.gene40000:CDS |
| TTACTAAAAGGACTTGCAAG+TGG | 0.586199 | 1.4:-59355628 | MS.gene40000:CDS |
| CACAGCCGTCGGAATTGGCG+AGG | 0.587987 | 1.4:+59355845 | None:intergenic |
| CCTCCACATACCGCCCTGCA+CGG | 0.588163 | 1.4:-59355888 | MS.gene40000:CDS |
| TTTGTTACACTAATGTGACT+GGG | 0.589924 | 1.4:-59355132 | MS.gene40000:CDS |
| CCTTCAGCCGAGTATTTCAT+CGG | 0.590645 | 1.4:-59355367 | MS.gene40000:CDS |
| TGGTGGAACAAAGATTAGTA+CGG | 0.593903 | 1.4:-59355269 | MS.gene40000:CDS |
| TTTCACGCCGATGAAATACT+CGG | 0.606909 | 1.4:+59355360 | None:intergenic |
| ACAGCCGTCGGAATTGGCGA+GGG | 0.612804 | 1.4:+59355846 | None:intergenic |
| CAACACCTGTGGTGTCACCC+CGG | 0.614141 | 1.4:-59355785 | MS.gene40000:CDS |
| ATGACGTTCAGTGGGACTAG+TGG | 0.614911 | 1.4:+59355964 | None:intergenic |
| CCGATGAAATACTCGGCTGA+AGG | 0.617301 | 1.4:+59355367 | None:intergenic |
| CCGTGCAGGGCGGTATGTGG+AGG | 0.617989 | 1.4:+59355888 | None:intergenic |
| TGCTGCAAGAAACATAAAGA+GGG | 0.632185 | 1.4:-59355179 | MS.gene40000:CDS |
| AGTTTGTCGTCCAAAGAGCA+AGG | 0.633294 | 1.4:+59354883 | None:intergenic |
| ATCACCCACACCGCCACCAG+CGG | 0.641652 | 1.4:-59355754 | MS.gene40000:CDS |
| GATCGACGATGACGTTCAGT+GGG | 0.650404 | 1.4:+59355956 | None:intergenic |
| GACGTTCAGTGGGACTAGTG+GGG | 0.659322 | 1.4:+59355966 | None:intergenic |
| AAAAGGCATCTAGGCAGTGG+AGG | 0.665153 | 1.4:+59354838 | None:intergenic |
| CTAACAGGATTGATCAAAAG+CGG | 0.665242 | 1.4:+59355418 | None:intergenic |
| TGCAAGAAACATAAAGAGGG+TGG | 0.665566 | 1.4:-59355176 | MS.gene40000:CDS |
| ATGTTGGGAAGAAAACCGTA+AGG | 0.671605 | 1.4:+59355472 | None:intergenic |
| GAAACAATCACCACAGCCGT+CGG | 0.679467 | 1.4:+59355834 | None:intergenic |
| GTGGGTGATGGAATTGTCCG+GGG | 0.682973 | 1.4:+59355768 | None:intergenic |
| GGCTGCAACAACAACACCTG+TGG | 0.687479 | 1.4:-59355796 | MS.gene40000:CDS |
| AACGTCATCGTCGATCTCGG+TGG | 0.719707 | 1.4:-59355949 | MS.gene40000:CDS |
| CTGAGCGACACCGTGCAGGG+CGG | 0.729304 | 1.4:+59355878 | None:intergenic |
| AAAGATTGTTGAGAGAAAGT+GGG | 0.736615 | 1.4:+59356069 | None:intergenic |
| TTGTTGTCTATAGACAACAA+CGG | 0.764264 | 1.4:-59355295 | MS.gene40000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGTGTAACAAAATTCAAA+AGG | + | chr1.4:59355834-59355853 | None:intergenic | 20.0% |
| !!! | TGTAATGAAGTATTGAAAAT+TGG | + | chr1.4:59354857-59354876 | None:intergenic | 20.0% |
| ! | AAATGAAGAAAAATAGGAGT+AGG | + | chr1.4:59354882-59354901 | None:intergenic | 25.0% |
| ! | AATGAAGAAAAATAGGAGTA+GGG | + | chr1.4:59354881-59354900 | None:intergenic | 25.0% |
| ! | AATGGTGAATATAAATGATG+AGG | - | chr1.4:59355944-59355963 | MS.gene40000:CDS | 25.0% |
| ! | TAATCTTGTGCAATTTGATT+TGG | - | chr1.4:59356043-59356062 | MS.gene40000:CDS | 25.0% |
| !! | GTAAATAGAAGCTTCTAAAA+CGG | + | chr1.4:59355742-59355761 | None:intergenic | 25.0% |
| AAAGATTGTTGAGAGAAAGT+GGG | + | chr1.4:59354907-59354926 | None:intergenic | 30.0% | |
| ATTTGTCTTTCCTCTTCAAA+GGG | - | chr1.4:59355456-59355475 | MS.gene40000:CDS | 30.0% | |
| TATTTGTCTTTCCTCTTCAA+AGG | - | chr1.4:59355455-59355474 | MS.gene40000:CDS | 30.0% | |
| TCTGAATCAAGAACAATGTT+GGG | + | chr1.4:59355519-59355538 | None:intergenic | 30.0% | |
| TGCAAAATAAGCTCAATAGT+AGG | + | chr1.4:59355888-59355907 | None:intergenic | 30.0% | |
| TGGGTGAAATGAAGAAAAAT+AGG | + | chr1.4:59354888-59354907 | None:intergenic | 30.0% | |
| TTATCAATCCAATCCTCTAA+TGG | - | chr1.4:59355261-59355280 | MS.gene40000:CDS | 30.0% | |
| TTGTTGTCTATAGACAACAA+CGG | - | chr1.4:59355678-59355697 | MS.gene40000:CDS | 30.0% | |
| TTTGTTACACTAATGTGACT+GGG | - | chr1.4:59355841-59355860 | MS.gene40000:CDS | 30.0% | |
| ! | AATGTGATTTGGAGGATATT+TGG | - | chr1.4:59355912-59355931 | MS.gene40000:CDS | 30.0% |
| ! | AGTCCTTTTAGTAAGAAAGT+TGG | + | chr1.4:59355339-59355358 | None:intergenic | 30.0% |
| ! | TTTTGCAGAACAATGTGATT+TGG | - | chr1.4:59355901-59355920 | MS.gene40000:CDS | 30.0% |
| ! | TTTTGTTACACTAATGTGAC+TGG | - | chr1.4:59355840-59355859 | MS.gene40000:CDS | 30.0% |
| !! | GATATTTGGTGCAAATTCAA+TGG | - | chr1.4:59355926-59355945 | MS.gene40000:CDS | 30.0% |
| !! | TCAAAGGGAGTTGTTTTATT+CGG | - | chr1.4:59355471-59355490 | MS.gene40000:CDS | 30.0% |
| ACAAGGACTTCAATTGTGAT+TGG | - | chr1.4:59356002-59356021 | MS.gene40000:CDS | 35.0% | |
| CACAATCAACCCATAGAAAT+TGG | + | chr1.4:59355050-59355069 | None:intergenic | 35.0% | |
| CTAACAGGATTGATCAAAAG+CGG | + | chr1.4:59355558-59355577 | None:intergenic | 35.0% | |
| CTTTCTCTCAACAATCTTTC+AGG | - | chr1.4:59354908-59354927 | MS.gene40000:CDS | 35.0% | |
| GAAAGATTGTTGAGAGAAAG+TGG | + | chr1.4:59354908-59354927 | None:intergenic | 35.0% | |
| GATTTGGCTGCTTCTAAATT+GGG | - | chr1.4:59356059-59356078 | MS.gene40000:CDS | 35.0% | |
| GCACCAACTTTCTTACTAAA+AGG | - | chr1.4:59355333-59355352 | MS.gene40000:CDS | 35.0% | |
| GTCTGAATCAAGAACAATGT+TGG | + | chr1.4:59355520-59355539 | None:intergenic | 35.0% | |
| TGAATGGTGGTAAGAATACA+AGG | - | chr1.4:59355985-59356004 | MS.gene40000:CDS | 35.0% | |
| TGCTGCAAGAAACATAAAGA+GGG | - | chr1.4:59355794-59355813 | MS.gene40000:CDS | 35.0% | |
| TGGTGGAACAAAGATTAGTA+CGG | - | chr1.4:59355704-59355723 | MS.gene40000:CDS | 35.0% | |
| TTACTAAAAGGACTTGCAAG+TGG | - | chr1.4:59355345-59355364 | MS.gene40000:CDS | 35.0% | |
| TTGTGTCTTGGATTTGTGAA+TGG | - | chr1.4:59355969-59355988 | MS.gene40000:CDS | 35.0% | |
| ! | AAACATAAAGAGGGTGGATT+CGG | - | chr1.4:59355803-59355822 | MS.gene40000:CDS | 35.0% |
| ! | TAAAACAACTCCCTTTGAAG+AGG | + | chr1.4:59355469-59355488 | None:intergenic | 35.0% |
| ! | TGATTTGGCTGCTTCTAAAT+TGG | - | chr1.4:59356058-59356077 | MS.gene40000:CDS | 35.0% |
| ! | TTGAGATGGAAGAGCAATTT+TGG | + | chr1.4:59355400-59355419 | None:intergenic | 35.0% |
| !! | AAAGGGAGTTGTTTTATTCG+GGG | - | chr1.4:59355473-59355492 | MS.gene40000:CDS | 35.0% |
| !! | AATGATGAGGTTTTGTGTCT+TGG | - | chr1.4:59355957-59355976 | MS.gene40000:CDS | 35.0% |
| !! | CAAAGGGAGTTGTTTTATTC+GGG | - | chr1.4:59355472-59355491 | MS.gene40000:CDS | 35.0% |
| !! | GGTTGATTTGAGCTTTGTAT+TGG | + | chr1.4:59354981-59355000 | None:intergenic | 35.0% |
| AGGACTTCAATTGTGATTGG+AGG | - | chr1.4:59356005-59356024 | MS.gene40000:CDS | 40.0% | |
| ATGTTGGGAAGAAAACCGTA+AGG | + | chr1.4:59355504-59355523 | None:intergenic | 40.0% | |
| CTGCTGCAAGAAACATAAAG+AGG | - | chr1.4:59355793-59355812 | MS.gene40000:CDS | 40.0% | |
| GGACTTCAATTGTGATTGGA+GGG | - | chr1.4:59356006-59356025 | MS.gene40000:CDS | 40.0% | |
| TCTATAGACAACAACGGTGT+TGG | - | chr1.4:59355684-59355703 | MS.gene40000:CDS | 40.0% | |
| TGCAAGAAACATAAAGAGGG+TGG | - | chr1.4:59355797-59355816 | MS.gene40000:CDS | 40.0% | |
| TGTCTTGGATTTGTGAATGG+TGG | - | chr1.4:59355972-59355991 | MS.gene40000:CDS | 40.0% | |
| TTTCACGCCGATGAAATACT+CGG | + | chr1.4:59355616-59355635 | None:intergenic | 40.0% | |
| ! | TCTTCCATCTCAACTAGCTT+CGG | - | chr1.4:59355407-59355426 | MS.gene40000:CDS | 40.0% |
| ! | TGCAGAACAATGTGATTTGG+AGG | - | chr1.4:59355904-59355923 | MS.gene40000:CDS | 40.0% |
| ! | TTTTGTGACTGGGAGAACTA+GGG | + | chr1.4:59354941-59354960 | None:intergenic | 40.0% |
| !! | AAGTTGGTGCACATGAGAAT+AGG | + | chr1.4:59355323-59355342 | None:intergenic | 40.0% |
| !! | GAAGCTTCTAAAACGGTGTA+AGG | + | chr1.4:59355735-59355754 | None:intergenic | 40.0% |
| AAACTGCGACATTTCGTCCT+GGG | + | chr1.4:59355293-59355312 | None:intergenic | 45.0% | |
| AAAGCAGAAGCTGTGCTAAC+AGG | + | chr1.4:59355573-59355592 | None:intergenic | 45.0% | |
| AAAGCCGAAGCTAGTTGAGA+TGG | + | chr1.4:59355414-59355433 | None:intergenic | 45.0% | |
| AATGTGACTGGGACACGATT+GGG | - | chr1.4:59355852-59355871 | MS.gene40000:CDS | 45.0% | |
| ACAGCTTCTGCTTTCTCACA+AGG | - | chr1.4:59355579-59355598 | MS.gene40000:CDS | 45.0% | |
| AGTTTGTCGTCCAAAGAGCA+AGG | + | chr1.4:59356093-59356112 | None:intergenic | 45.0% | |
| ATCTCGGTGGCCAATTTCTA+TGG | - | chr1.4:59355037-59355056 | MS.gene40000:CDS | 45.0% | |
| CCTTCAGCCGAGTATTTCAT+CGG | - | chr1.4:59355606-59355625 | MS.gene40000:CDS | 45.0% | |
| GGTTGAAGCCATTAGAGGAT+TGG | + | chr1.4:59355272-59355291 | None:intergenic | 45.0% | |
| GTTCTCCCAGTCACAAAAGA+TGG | - | chr1.4:59354943-59354962 | MS.gene40000:CDS | 45.0% | |
| TAATGTGACTGGGACACGAT+TGG | - | chr1.4:59355851-59355870 | MS.gene40000:CDS | 45.0% | |
| TCTCGGTGGCCAATTTCTAT+GGG | - | chr1.4:59355038-59355057 | MS.gene40000:CDS | 45.0% | |
| TGTTTCTCATCACCAAAGCC+TGG | - | chr1.4:59355156-59355175 | MS.gene40000:CDS | 45.0% | |
| TTATTCGGGGATGGACCTTA+CGG | - | chr1.4:59355486-59355505 | MS.gene40000:CDS | 45.0% | |
| ! | ATAGACAACAACGGTGTTGG+TGG | - | chr1.4:59355687-59355706 | MS.gene40000:CDS | 45.0% |
| ! | CTTTTGTGACTGGGAGAACT+AGG | + | chr1.4:59354942-59354961 | None:intergenic | 45.0% |
| ! | GGATTTAGCTCCTTGCTCTT+TGG | - | chr1.4:59356080-59356099 | MS.gene40000:CDS | 45.0% |
| ! | TAGCGCCATCTTTTGTGACT+GGG | + | chr1.4:59354951-59354970 | None:intergenic | 45.0% |
| !! | GGAGTTGTTTTATTCGGGGA+TGG | - | chr1.4:59355477-59355496 | MS.gene40000:CDS | 45.0% |
| !! | TGTGATTGGAGGGTATCAGT+TGG | - | chr1.4:59356016-59356035 | MS.gene40000:CDS | 45.0% |
| AGATCGACGATGACGTTCAG+TGG | + | chr1.4:59355021-59355040 | None:intergenic | 50.0% | |
| ATCTTCAGCAAGTTCACCGC+TGG | + | chr1.4:59355238-59355257 | None:intergenic | 50.0% | |
| ATGACGTTCAGTGGGACTAG+TGG | + | chr1.4:59355012-59355031 | None:intergenic | 50.0% | |
| CCGATGAAATACTCGGCTGA+AGG | + | chr1.4:59355609-59355628 | None:intergenic | 50.0% | |
| CTGAACGTCATCGTCGATCT+CGG | - | chr1.4:59355021-59355040 | MS.gene40000:CDS | 50.0% | |
| GAAACAATCACCACAGCCGT+CGG | + | chr1.4:59355142-59355161 | None:intergenic | 50.0% | |
| GAAACTGCGACATTTCGTCC+TGG | + | chr1.4:59355294-59355313 | None:intergenic | 50.0% | |
| GATCGACGATGACGTTCAGT+GGG | + | chr1.4:59355020-59355039 | None:intergenic | 50.0% | |
| TCCTCTAATGGCTTCAACCC+AGG | - | chr1.4:59355273-59355292 | MS.gene40000:CDS | 50.0% | |
| ! | ACTGGGAGAACTAGGGCTTT+TGG | + | chr1.4:59354934-59354953 | None:intergenic | 50.0% |
| ! | GTAGCGCCATCTTTTGTGAC+TGG | + | chr1.4:59354952-59354971 | None:intergenic | 50.0% |
| ! | TCCTGGGTTGAAGCCATTAG+AGG | + | chr1.4:59355277-59355296 | None:intergenic | 50.0% |
| ! | TGACGTTCAGTGGGACTAGT+GGG | + | chr1.4:59355011-59355030 | None:intergenic | 50.0% |
| ! | TTGTTGTTGCAGCCAGGCTT+TGG | + | chr1.4:59355171-59355190 | None:intergenic | 50.0% |
| !! | GATTGGAGGGTATCAGTTGG+AGG | - | chr1.4:59356019-59356038 | MS.gene40000:CDS | 50.0% |
| !! | GTGTGGGTGATGGAATTGTC+CGG | + | chr1.4:59355210-59355229 | None:intergenic | 50.0% |
| !! | TGTGGGTGATGGAATTGTCC+GGG | + | chr1.4:59355209-59355228 | None:intergenic | 50.0% |
| AACGTCATCGTCGATCTCGG+TGG | - | chr1.4:59355024-59355043 | MS.gene40000:CDS | 55.0% | |
| ATCACCACAGCCGTCGGAAT+TGG | + | chr1.4:59355136-59355155 | None:intergenic | 55.0% | |
| GGACTTGCAAGTGGTGCTTC+TGG | - | chr1.4:59355354-59355373 | MS.gene40000:CDS | 55.0% | |
| GGCTGCAACAACAACACCTG+TGG | - | chr1.4:59355177-59355196 | MS.gene40000:CDS | 55.0% | |
| TGCAAGTGGTGCTTCTGGCA+TGG | - | chr1.4:59355359-59355378 | MS.gene40000:CDS | 55.0% | |
| TTCAGCAAGTTCACCGCTGG+TGG | + | chr1.4:59355235-59355254 | None:intergenic | 55.0% | |
| ! | GACGTTCAGTGGGACTAGTG+GGG | + | chr1.4:59355010-59355029 | None:intergenic | 55.0% |
| ! | GTGGGACTAGTGGGGTTCTT+TGG | + | chr1.4:59355002-59355021 | None:intergenic | 55.0% |
| !! | CAGGTGTTGTTGTTGCAGCC+AGG | + | chr1.4:59355177-59355196 | None:intergenic | 55.0% |
| !! | GGAATTGGCGAGGGAGCATT+GGG | + | chr1.4:59355121-59355140 | None:intergenic | 55.0% |
| !! | GTGGGTGATGGAATTGTCCG+GGG | + | chr1.4:59355208-59355227 | None:intergenic | 55.0% |
| ACAGCCGTCGGAATTGGCGA+GGG | + | chr1.4:59355130-59355149 | None:intergenic | 60.0% | |
| AGCAAGTTCACCGCTGGTGG+CGG | + | chr1.4:59355232-59355251 | None:intergenic | 60.0% | |
| AGTGGTGCTTCTGGCATGGC+TGG | - | chr1.4:59355363-59355382 | MS.gene40000:CDS | 60.0% | |
| ATTGTCCGGGGTGACACCAC+AGG | + | chr1.4:59355196-59355215 | None:intergenic | 60.0% | |
| TGCTCCCTCGCCAATTCCGA+CGG | - | chr1.4:59355123-59355142 | MS.gene40000:CDS | 60.0% | |
| ! | CGGAATTGGCGAGGGAGCAT+TGG | + | chr1.4:59355122-59355141 | None:intergenic | 60.0% |
| !! | CAACACCTGTGGTGTCACCC+CGG | - | chr1.4:59355188-59355207 | MS.gene40000:CDS | 60.0% |
| !! | GCTTCTGGCATGGCTGGTCT+TGG | - | chr1.4:59355369-59355388 | MS.gene40000:CDS | 60.0% |
| ACACCGTGCAGGGCGGTATG+TGG | + | chr1.4:59355091-59355110 | None:intergenic | 65.0% | |
| ATCACCCACACCGCCACCAG+CGG | - | chr1.4:59355219-59355238 | MS.gene40000:CDS | 65.0% | |
| CACAGCCGTCGGAATTGGCG+AGG | + | chr1.4:59355131-59355150 | None:intergenic | 65.0% | |
| CCTCCACATACCGCCCTGCA+CGG | - | chr1.4:59355085-59355104 | MS.gene40000:CDS | 65.0% | |
| CTCGCCAATTCCGACGGCTG+TGG | - | chr1.4:59355129-59355148 | MS.gene40000:CDS | 65.0% | |
| !! | TTCACCGCTGGTGGCGGTGT+GGG | + | chr1.4:59355226-59355245 | None:intergenic | 65.0% |
| CCGTGCAGGGCGGTATGTGG+AGG | + | chr1.4:59355088-59355107 | None:intergenic | 70.0% | |
| CTGAGCGACACCGTGCAGGG+CGG | + | chr1.4:59355098-59355117 | None:intergenic | 70.0% | |
| GGGCTGAGCGACACCGTGCA+GGG | + | chr1.4:59355101-59355120 | None:intergenic | 70.0% | |
| TGGGCTGAGCGACACCGTGC+AGG | + | chr1.4:59355102-59355121 | None:intergenic | 70.0% | |
| !! | GCTGGTGGCGGTGTGGGTGA+TGG | + | chr1.4:59355220-59355239 | None:intergenic | 70.0% |
| !! | GTTCACCGCTGGTGGCGGTG+TGG | + | chr1.4:59355227-59355246 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 59354847 | 59356148 | 59354847 | ID=MS.gene40000 |
| chr1.4 | mRNA | 59354847 | 59356148 | 59354847 | ID=MS.gene40000.t1;Parent=MS.gene40000 |
| chr1.4 | exon | 59354847 | 59356148 | 59354847 | ID=MS.gene40000.t1.exon1;Parent=MS.gene40000.t1 |
| chr1.4 | CDS | 59354847 | 59356148 | 59354847 | ID=cds.MS.gene40000.t1;Parent=MS.gene40000.t1 |
| Gene Sequence |
| Protein sequence |
