AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40013

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40013.t1 MTR_4g102520 97.265 585 14 2 1 584 1 584 0.0 1191
MS.gene40013.t1 MTR_1g072740 98.131 535 9 1 1 535 1 534 0.0 1089
MS.gene40013.t1 MTR_8g467040 32.903 310 194 4 282 581 107 412 2.89e-50 179
MS.gene40013.t1 MTR_4g119620 28.267 329 209 11 270 584 6 321 5.78e-32 126
MS.gene40013.t1 MTR_1g072140 25.000 308 218 8 281 584 14 312 3.99e-28 115
MS.gene40013.t1 MTR_1g072140 24.335 263 185 8 286 542 61 315 1.53e-17 84.3
MS.gene40013.t1 MTR_7g086360 26.415 318 221 9 268 581 32 340 4.15e-25 107
MS.gene40013.t1 MTR_8g092620 27.687 307 203 10 282 584 6 297 1.14e-24 104
MS.gene40013.t1 MTR_8g092620 24.615 260 143 9 283 496 47 299 4.24e-11 64.7
MS.gene40013.t1 MTR_4g104380 25.962 312 206 8 282 584 218 513 3.31e-24 107
MS.gene40013.t1 MTR_4g104380 33.019 106 65 3 273 374 289 392 8.03e-12 68.2
MS.gene40013.t1 MTR_8g092620 27.541 305 202 10 282 582 13 302 2.86e-23 101
MS.gene40013.t1 MTR_3g075400 26.471 306 214 8 281 584 212 508 4.78e-23 103
MS.gene40013.t1 MTR_2g436510 25.974 308 196 12 291 584 167 456 5.50e-18 87.4
MS.gene40013.t1 MTR_7g076230 25.784 287 189 9 292 560 367 647 1.05e-17 87.4
MS.gene40013.t1 MTR_8g092620 28.037 214 139 8 282 491 6 208 1.29e-16 79.7
MS.gene40013.t1 MTR_6g004040 29.825 171 111 6 285 453 92 255 1.57e-16 83.6
MS.gene40013.t1 MTR_6g004040 22.917 288 198 10 290 565 12 287 2.85e-13 73.2
MS.gene40013.t1 MTR_6g004040 24.339 189 136 4 396 584 31 212 6.01e-12 68.9
MS.gene40013.t1 MTR_6g004040 31.538 130 80 4 284 410 133 256 2.28e-11 67.4
MS.gene40013.t1 MTR_1g069345 24.691 324 227 11 267 584 191 503 8.30e-16 80.9
MS.gene40013.t1 MTR_2g012630 29.545 176 114 7 285 458 91 258 1.61e-15 80.9
MS.gene40013.t1 MTR_2g012630 23.232 198 145 4 387 584 21 211 4.07e-12 69.7
MS.gene40013.t1 MTR_2g012630 22.526 293 199 10 287 565 8 286 1.76e-11 67.8
MS.gene40013.t1 MTR_2g012630 29.545 176 114 7 285 458 91 258 2.05e-15 80.5
MS.gene40013.t1 MTR_2g012630 23.232 198 145 4 387 584 21 211 6.15e-12 69.3
MS.gene40013.t1 MTR_2g012630 22.526 293 199 10 287 565 8 286 2.46e-11 67.4
MS.gene40013.t1 MTR_1g097140 25.329 304 204 14 287 584 73 359 2.71e-15 78.6
MS.gene40013.t1 MTR_5g090420 21.338 314 234 8 276 584 243 548 2.25e-14 76.6
MS.gene40013.t1 MTR_1g054300 22.835 381 204 10 285 584 110 481 2.45e-14 76.3
MS.gene40013.t1 MTR_3g052220 23.197 319 219 10 283 584 202 511 2.87e-13 72.8
MS.gene40013.t1 MTR_2g060520 21.429 322 228 8 283 584 115 431 2.39e-12 69.7
MS.gene40013.t1 MTR_3g116500 23.810 315 216 10 289 584 60 369 4.80e-12 68.2
MS.gene40013.t1 MTR_3g116500 25.778 225 151 7 373 584 38 259 2.17e-11 65.1
MS.gene40013.t1 MTR_8g106790 22.662 278 184 12 282 539 54 320 3.03e-11 65.5
MS.gene40013.t1 MTR_1g034785 24.339 189 117 5 285 451 109 293 5.58e-11 65.5
MS.gene40013.t1 MTR_2g060520 21.711 304 213 8 301 584 13 311 7.82e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40013.t1 AT1G10580 77.094 585 120 4 1 584 1 572 0.0 933
MS.gene40013.t1 AT5G54520 32.602 319 205 3 274 584 140 456 1.43e-53 189
MS.gene40013.t1 AT3G49660 25.000 312 216 9 281 584 11 312 4.82e-28 115
MS.gene40013.t1 AT3G49660 28.311 219 146 6 286 499 105 317 3.97e-24 103
MS.gene40013.t1 AT5G64730 27.508 309 201 11 282 584 6 297 6.93e-27 111
MS.gene40013.t1 AT4G15900 29.642 307 184 12 292 584 174 462 5.21e-25 108
MS.gene40013.t1 AT2G43770 26.117 291 207 7 291 578 50 335 1.79e-24 105
MS.gene40013.t1 AT4G02730 24.437 311 217 10 281 584 30 329 2.83e-23 101
MS.gene40013.t1 AT3G16650 28.664 307 186 13 292 584 168 455 7.63e-21 96.3
MS.gene40013.t1 AT5G50230 24.658 292 196 7 282 564 213 489 1.37e-19 92.8
MS.gene40013.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.20e-17 87.0
MS.gene40013.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.20e-17 87.0
MS.gene40013.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.20e-17 87.0
MS.gene40013.t1 AT1G61210 31.429 175 112 6 285 458 91 258 6.50e-17 85.1
MS.gene40013.t1 AT1G61210 25.357 280 183 10 292 567 13 270 9.21e-15 78.6
MS.gene40013.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.29e-11 68.2
MS.gene40013.t1 AT1G61210 31.429 175 112 6 285 458 91 258 8.61e-17 85.1
MS.gene40013.t1 AT1G61210 25.357 280 183 10 292 567 13 270 1.49e-14 77.8
MS.gene40013.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.54e-11 68.2
MS.gene40013.t1 AT1G61210 31.429 175 112 6 285 458 91 258 9.56e-17 84.7
MS.gene40013.t1 AT1G61210 25.357 280 183 10 292 567 13 270 1.62e-14 77.8
MS.gene40013.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.65e-11 68.2
MS.gene40013.t1 AT5G23430 22.924 301 201 12 287 578 9 287 1.93e-16 83.6
MS.gene40013.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.41e-14 77.8
MS.gene40013.t1 AT5G23430 24.479 192 132 4 396 584 31 212 6.01e-12 69.3
MS.gene40013.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.10e-11 67.0
MS.gene40013.t1 AT5G23430 22.924 301 201 12 287 578 9 287 1.93e-16 83.6
MS.gene40013.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.41e-14 77.8
MS.gene40013.t1 AT5G23430 24.479 192 132 4 396 584 31 212 6.01e-12 69.3
MS.gene40013.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.10e-11 67.0
MS.gene40013.t1 AT5G23430 22.924 301 201 12 287 578 9 287 1.95e-16 83.6
MS.gene40013.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.42e-14 77.8
MS.gene40013.t1 AT5G23430 24.479 192 132 4 396 584 31 212 6.33e-12 69.3
MS.gene40013.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.24e-11 67.0
MS.gene40013.t1 AT5G43920 24.688 320 218 12 278 584 204 513 2.93e-16 82.4
MS.gene40013.t1 AT5G08390 23.551 276 193 9 287 561 9 267 4.20e-16 82.4
MS.gene40013.t1 AT5G08390 32.000 150 94 5 285 432 92 235 1.90e-15 80.5
MS.gene40013.t1 AT5G08390 32.283 127 83 3 284 410 133 256 1.83e-12 70.9
MS.gene40013.t1 AT5G08390 24.479 192 132 4 396 584 31 212 4.22e-12 69.7
MS.gene40013.t1 AT1G15440 26.009 223 157 5 292 510 347 565 2.07e-15 80.5
MS.gene40013.t1 AT1G15440 26.009 223 157 5 292 510 387 605 2.73e-15 80.1
MS.gene40013.t1 AT1G11160 27.684 177 120 6 285 460 49 218 5.88e-15 79.0
MS.gene40013.t1 AT1G11160 25.150 167 118 4 418 584 10 169 1.73e-11 67.8
MS.gene40013.t1 AT1G11160 30.534 131 80 6 284 410 90 213 5.21e-11 66.2
MS.gene40013.t1 AT1G11160 27.684 177 120 6 285 460 91 260 5.95e-15 79.0
MS.gene40013.t1 AT1G11160 24.399 291 196 9 287 565 8 286 7.64e-15 78.6
MS.gene40013.t1 AT1G11160 24.868 189 135 4 396 584 30 211 1.69e-13 74.3
MS.gene40013.t1 AT1G11160 30.534 131 80 6 284 410 132 255 5.68e-11 66.2
MS.gene40013.t1 AT1G11160 27.684 177 120 6 285 460 91 260 6.37e-15 79.0
MS.gene40013.t1 AT1G11160 24.399 291 196 9 287 565 8 286 8.05e-15 78.6
MS.gene40013.t1 AT1G11160 24.868 189 135 4 396 584 30 211 1.66e-13 74.3
MS.gene40013.t1 AT1G11160 30.534 131 80 6 284 410 132 255 5.71e-11 66.2
MS.gene40013.t1 AT1G11160 27.684 177 120 6 285 460 91 260 6.77e-15 79.0
MS.gene40013.t1 AT1G11160 24.399 291 196 9 287 565 8 286 8.78e-15 78.6
MS.gene40013.t1 AT1G11160 24.868 189 135 4 396 584 30 211 1.57e-13 74.3
MS.gene40013.t1 AT1G11160 30.534 131 80 6 284 410 132 255 5.77e-11 66.2
MS.gene40013.t1 AT5G16750 25.444 169 118 5 289 452 55 220 8.95e-13 72.0
MS.gene40013.t1 AT2G41500 23.228 254 179 7 335 584 252 493 1.23e-12 70.9
MS.gene40013.t1 AT2G41500 25.472 212 152 6 286 496 331 537 3.69e-12 69.3
MS.gene40013.t1 AT2G41500 23.228 254 179 7 335 584 252 493 1.23e-12 70.9
MS.gene40013.t1 AT2G41500 25.472 212 152 6 286 496 331 537 3.69e-12 69.3
MS.gene40013.t1 AT3G18130 24.535 269 176 11 291 539 60 321 1.46e-12 69.3
MS.gene40013.t1 AT5G52820 24.161 298 205 10 291 584 191 471 3.49e-12 69.3
MS.gene40013.t1 AT2G33340 25.197 254 174 9 285 532 255 498 1.11e-11 67.8
MS.gene40013.t1 AT2G33340 25.197 254 174 9 285 532 255 498 1.11e-11 67.8
MS.gene40013.t1 AT1G48630 24.535 269 178 11 291 541 60 321 1.63e-11 66.2
MS.gene40013.t1 AT2G33340 27.011 174 117 6 285 453 255 423 1.69e-11 67.4
MS.gene40013.t1 AT5G60940 23.125 320 224 9 282 584 111 425 2.41e-11 66.6
MS.gene40013.t1 AT5G60940 23.125 320 224 9 282 584 19 333 4.19e-11 65.1
MS.gene40013.t1 AT1G73720 24.138 319 215 12 283 584 201 509 4.56e-11 65.9

Find 153 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACGGGTTTGGAGAGGGTTT+TGG 0.229868 1.4:-59542047 None:intergenic
GTGAGGGTAAGTGTTGGTTC+TGG 0.241552 1.4:+59543490 MS.gene40013:CDS
ATCGAAATCACCGACGTAAT+TGG 0.272574 1.4:-59542287 None:intergenic
AGCATGGTGTCGTCGACTTT+GGG 0.273615 1.4:-59542004 None:intergenic
TTACTCGCCTTAGCATCTTT+TGG 0.281128 1.4:-59542697 None:intergenic
ACATTCTGTTTATCCTCATC+TGG 0.288775 1.4:-59543054 None:intergenic
TAAATGTATGAGGACTTATA+TGG 0.290113 1.4:+59542873 MS.gene40013:CDS
CAAGAGTATGATCAGCATTT+GGG 0.309524 1.4:+59543141 MS.gene40013:CDS
TGGATACTAAGGTTAAGATT+TGG 0.323671 1.4:+59542833 MS.gene40013:CDS
GGCGAAGAGAAGAGTGGTTT+TGG 0.323999 1.4:+59542586 MS.gene40013:CDS
TCCCTCAGGGTATGGGAATA+TGG 0.330247 1.4:+59543228 MS.gene40013:CDS
TTGGCTCGCCGCTCAGAGTT+TGG 0.331077 1.4:+59543329 MS.gene40013:CDS
GGATACTAAGGTTAAGATTT+GGG 0.331103 1.4:+59542834 MS.gene40013:CDS
GGTGAGATGGGCCTTGAATC+GGG 0.335801 1.4:-59542123 None:intergenic
TGATAAGAATATTAAGTATT+GGG 0.340716 1.4:+59542963 MS.gene40013:CDS
GTTCAGTGGGATATGAATAC+TGG 0.343180 1.4:+59543108 MS.gene40013:CDS
TTGAGTTGGGTCGACGGGTT+TGG 0.349448 1.4:-59542059 None:intergenic
CAGCTCTGGGCCCCGATTCA+AGG 0.351014 1.4:+59542112 MS.gene40013:CDS
TGAGGGTAAGTGTTGGTTCT+GGG 0.355149 1.4:+59543491 MS.gene40013:CDS
GAGCATGGTGTCGTCGACTT+TGG 0.357237 1.4:-59542005 None:intergenic
CGTTGTTGTCGTCGGTGTAT+TGG 0.360399 1.4:-59541904 None:intergenic
TTTGATTCTGTCTGCTGGTA+TGG 0.366420 1.4:+59542813 MS.gene40013:CDS
TCAAGAGTATGATCAGCATT+TGG 0.367145 1.4:+59543140 MS.gene40013:CDS
AACAACCATGCCTCAGAAGC+TGG 0.373950 1.4:-59542463 None:intergenic
TGGGGCATTCGAAAGCTGTT+AGG 0.375620 1.4:+59542893 MS.gene40013:CDS
CGAAAGGGGTTTCTGCGATT+AGG 0.375961 1.4:+59542770 MS.gene40013:CDS
TTTGCTGGACATATTGTTGC+TGG 0.377860 1.4:+59543411 MS.gene40013:CDS
GGGTGAGATGGGCCTTGAAT+CGG 0.384210 1.4:-59542124 None:intergenic
CGATATAACCTGCCCCGTTT+CGG 0.387388 1.4:-59542989 None:intergenic
TTGTGCTATAAATAAGTATC+TGG 0.390504 1.4:-59543356 None:intergenic
CACTTCACACCAATGCCAAT+TGG 0.395265 1.4:+59543310 MS.gene40013:CDS
ACCCTACCTACGACCAGCTC+TGG 0.397227 1.4:+59542098 MS.gene40013:CDS
GGGATTATCAAGGTAGGTCT+TGG 0.401179 1.4:+59542665 MS.gene40013:CDS
TTTGGGGTCGGCGACGGTTA+GGG 0.403402 1.4:-59542029 None:intergenic
GTAAGTGTTGGTTCTGGGAT+TGG 0.407813 1.4:+59543496 MS.gene40013:CDS
CTTGAATCGGGGCCCAGAGC+TGG 0.409734 1.4:-59542111 None:intergenic
GCAAAGTAGCGACGTGTGGC+TGG 0.411956 1.4:+59543598 MS.gene40013:CDS
ATGATAAGAATATTAAGTAT+TGG 0.420715 1.4:+59542962 MS.gene40013:CDS
AGAATCAAATGACCCGAATT+CGG 0.425737 1.4:-59542799 None:intergenic
TCTTCTTCTGTTCACGGTGT+GGG 0.426132 1.4:-59542348 None:intergenic
GAACGGGAAGGGAGGAGACG+AGG 0.429345 1.4:-59541974 None:intergenic
AAGTATTGGGATACCGAAAC+GGG 0.432545 1.4:+59542976 MS.gene40013:CDS
ATGACAAGTCCCTCAGGGTA+TGG 0.434880 1.4:+59543220 MS.gene40013:CDS
GAGTCCTTGGGCTGGGAAGA+AGG 0.437077 1.4:+59542498 MS.gene40013:CDS
CTCTTCTTCTGTTCACGGTG+TGG 0.437638 1.4:-59542349 None:intergenic
TCAATACTGGTAAATGTATG+AGG 0.444000 1.4:+59542863 MS.gene40013:CDS
GCCCAGAGCTGGTCGTAGGT+AGG 0.447192 1.4:-59542100 None:intergenic
GGGTTAAGCTTCACAACATA+AGG 0.453310 1.4:-59543033 None:intergenic
GGTCATTTGATTCTGTCTGC+TGG 0.457625 1.4:+59542808 MS.gene40013:CDS
AAATGTATGAGGACTTATAT+GGG 0.458158 1.4:+59542874 MS.gene40013:CDS
TGAAGAATAAGAAGAGTCCT+TGG 0.458759 1.4:+59542485 MS.gene40013:CDS
GTCTGCTGGTATGGATACTA+AGG 0.461762 1.4:+59542822 MS.gene40013:CDS
GAGGAGCACGCGAAGAAGAA+AGG 0.463092 1.4:+59542565 MS.gene40013:CDS
TGGTTGCGAGTGGCATCCTC+TGG 0.463609 1.4:+59543569 MS.gene40013:CDS
GGGCGGCGGAACGGGAAGGG+AGG 0.465040 1.4:-59541982 None:intergenic
TCTTTAGCGTAGGGGTGAGA+TGG 0.468815 1.4:-59542136 None:intergenic
TAGCGACGTGTGGCTGGGAC+GGG 0.469608 1.4:+59543604 MS.gene40013:CDS
CCCAGAGCTGGTCGTAGGTA+GGG 0.477469 1.4:-59542099 None:intergenic
CTGATATACTTTATAACTAC+AGG 0.479452 1.4:-59543255 None:intergenic
AGGTGTTGAGTTGGGTCGAC+GGG 0.484299 1.4:-59542064 None:intergenic
TGTTGCTGGATATGCTTGTC+AGG 0.489525 1.4:+59543425 MS.gene40013:CDS
CCAGTGATGACAAGTCCCTC+AGG 0.489956 1.4:+59543214 MS.gene40013:CDS
GCTAAACCGTCTTTAGCGTA+GGG 0.490697 1.4:-59542145 None:intergenic
AGGCGATAAGGTGTTGAGTT+GGG 0.492099 1.4:-59542072 None:intergenic
GGCATTGGTGTGAAGTGAAA+TGG 0.492494 1.4:-59543304 None:intergenic
GGGGCATTCGAAAGCTGTTA+GGG 0.493324 1.4:+59542894 MS.gene40013:CDS
CATTCTGTTTATCCTCATCT+GGG 0.502022 1.4:-59543053 None:intergenic
AGCGGCGAGCCAATTGGCAT+TGG 0.504559 1.4:-59543319 None:intergenic
CCCTACCTACGACCAGCTCT+GGG 0.505645 1.4:+59542099 MS.gene40013:CDS
CGAAGAGATTGGTACATACA+TGG 0.505690 1.4:+59542737 MS.gene40013:CDS
TGGGTCGACGGGTTTGGAGA+GGG 0.510892 1.4:-59542053 None:intergenic
ACTCTGAGCGGCGAGCCAAT+TGG 0.513719 1.4:-59543325 None:intergenic
TAAGTATTGGGATACCGAAA+CGG 0.518461 1.4:+59542975 MS.gene40013:CDS
CATGTATGTACCAATCTCTT+CGG 0.518553 1.4:-59542736 None:intergenic
ATCTCAAAACCCTAATTCAC+CGG 0.519645 1.4:+59541940 MS.gene40013:CDS
CAAAGTAGCGACGTGTGGCT+GGG 0.520964 1.4:+59543599 MS.gene40013:CDS
AGGTAGGGTTGAAGGCGATA+AGG 0.521290 1.4:-59542084 None:intergenic
CTTTAGCGTAGGGGTGAGAT+GGG 0.521850 1.4:-59542135 None:intergenic
TGCTAAACCGTCTTTAGCGT+AGG 0.523130 1.4:-59542146 None:intergenic
TTGGGATACCGAAACGGGGC+AGG 0.523429 1.4:+59542981 MS.gene40013:CDS
AAGGTGTTGAGTTGGGTCGA+CGG 0.523575 1.4:-59542065 None:intergenic
GTAGCGACGTGTGGCTGGGA+CGG 0.525013 1.4:+59543603 MS.gene40013:CDS
TTGGGTCGACGGGTTTGGAG+AGG 0.527855 1.4:-59542054 None:intergenic
GTTATATCGACTTTCTCCAC+TGG 0.528482 1.4:+59543003 MS.gene40013:CDS
GATTGAAAACCCAGCTTCTG+AGG 0.529050 1.4:+59542453 MS.gene40013:CDS
CTGGTCGTAGGTAGGGTTGA+AGG 0.530035 1.4:-59542092 None:intergenic
AAGGCGATAAGGTGTTGAGT+TGG 0.533837 1.4:-59542073 None:intergenic
CTGTTATATACCGAAGAGAT+TGG 0.537473 1.4:+59542726 MS.gene40013:CDS
CCTGAGGGACTTGTCATCAC+TGG 0.537996 1.4:-59543214 None:intergenic
TGATAAGAAGATTGTTCAGT+GGG 0.538924 1.4:+59543095 MS.gene40013:CDS
ATAAGAAGAGTCCTTGGGCT+GGG 0.539663 1.4:+59542491 MS.gene40013:CDS
ACAACCATGCCTCAGAAGCT+GGG 0.540280 1.4:-59542462 None:intergenic
TGTCACGAAGGCGTTACCAT+TGG 0.543297 1.4:+59543549 MS.gene40013:CDS
AAAACCCAGCTTCTGAGGCA+TGG 0.546170 1.4:+59542458 MS.gene40013:CDS
CATGGAGTGGACATACGAAA+GGG 0.549842 1.4:+59542755 MS.gene40013:CDS
TGACAAGTCCCTCAGGGTAT+GGG 0.550285 1.4:+59543221 MS.gene40013:CDS
CCTTCCTTCTTCCCAGCCCA+AGG 0.553576 1.4:-59542502 None:intergenic
AAGAAAGGCGAAGAGAAGAG+TGG 0.555351 1.4:+59542580 MS.gene40013:CDS
TGCTGTTCATCGAAGAGGAA+AGG 0.559395 1.4:-59542220 None:intergenic
TGCGCCTCCAAAAGATGCTA+AGG 0.561249 1.4:+59542690 MS.gene40013:CDS
GGGACGGGTTGATAAAGTAC+TGG 0.561357 1.4:+59543619 MS.gene40013:CDS
GTGAGATGGGCCTTGAATCG+GGG 0.561821 1.4:-59542122 None:intergenic
GTCTGGGTCGTTGTTGTCGT+CGG 0.562377 1.4:-59541912 None:intergenic
GAAGAATAAGAAGAGTCCTT+GGG 0.563353 1.4:+59542486 MS.gene40013:CDS
CCTTGGGCTGGGAAGAAGGA+AGG 0.564772 1.4:+59542502 MS.gene40013:CDS
GCATGGTGTCGTCGACTTTG+GGG 0.565100 1.4:-59542003 None:intergenic
GGGAAGAAGGAAGGTTTACA+AGG 0.566983 1.4:+59542511 MS.gene40013:CDS
AATAAGAAGAGTCCTTGGGC+TGG 0.567302 1.4:+59542490 MS.gene40013:CDS
GACATGACAAAACGTCCATC+CGG 0.567963 1.4:-59543459 None:intergenic
TGTCATGTCGGGTGATGGTG+AGG 0.571408 1.4:+59543473 MS.gene40013:CDS
ATTGTTATCGACAAAAGTAA+TGG 0.571823 1.4:-59543175 None:intergenic
AAAGAGGAAAGGGATTATCA+AGG 0.572402 1.4:+59542655 MS.gene40013:CDS
CGGGAAGGGAGGAGACGAGG+TGG 0.573173 1.4:-59541971 None:intergenic
GGCGACGGTTAGGGCGAGCA+TGG 0.589400 1.4:-59542020 None:intergenic
AAGAGTATGATCAGCATTTG+GGG 0.593826 1.4:+59543142 MS.gene40013:CDS
TTCAGTGGGATATGAATACT+GGG 0.594900 1.4:+59543109 MS.gene40013:CDS
TTATATCGACTTTCTCCACT+GGG 0.596005 1.4:+59543004 MS.gene40013:CDS
GCTACTTTGCTCTGTTCCAG+AGG 0.598337 1.4:-59543585 None:intergenic
AGATACTTATTTATAGCACA+AGG 0.600630 1.4:+59543358 MS.gene40013:CDS
GCGTTACCATTGGTTGCGAG+TGG 0.608524 1.4:+59543559 MS.gene40013:CDS
AGGAAAGGGATTATCAAGGT+AGG 0.608842 1.4:+59542659 MS.gene40013:CDS
ACATGGAGTGGACATACGAA+AGG 0.611220 1.4:+59542754 MS.gene40013:CDS
GTCATGTCGGGTGATGGTGA+GGG 0.625539 1.4:+59543474 MS.gene40013:CDS
CGGGGCCCAGAGCTGGTCGT+AGG 0.625795 1.4:-59542104 None:intergenic
ACCATATTCCCATACCCTGA+GGG 0.627603 1.4:-59543229 None:intergenic
CATGGTGTCGTCGACTTTGG+GGG 0.627690 1.4:-59542002 None:intergenic
CAGTGATGACAAGTCCCTCA+GGG 0.630201 1.4:+59543215 MS.gene40013:CDS
AGGATGCCACTCGCAACCAA+TGG 0.631424 1.4:-59543565 None:intergenic
TGTTATGCTGTTCATCGAAG+AGG 0.631441 1.4:-59542225 None:intergenic
TCTCACCCCTACGCTAAAGA+CGG 0.636996 1.4:+59542139 MS.gene40013:CDS
GCTAAAGACGGTTTAGCACA+AGG 0.641977 1.4:+59542151 MS.gene40013:CDS
AGATTGGTACATACATGGAG+TGG 0.644932 1.4:+59542742 MS.gene40013:CDS
CAGAGCAAAGTAGCGACGTG+TGG 0.656241 1.4:+59543594 MS.gene40013:CDS
GTGATAAGAAGATTGTTCAG+TGG 0.659340 1.4:+59543094 MS.gene40013:CDS
AACAACAACGAAGATGAAGA+CGG 0.659994 1.4:+59542424 MS.gene40013:CDS
CCGACGTAATTGGAAGCAGA+CGG 0.664084 1.4:-59542277 None:intergenic
AATGTATGAGGACTTATATG+GGG 0.667045 1.4:+59542875 MS.gene40013:CDS
TGTGAAGCTTAACCCAGATG+AGG 0.669296 1.4:+59543041 MS.gene40013:CDS
GATACTTATTTATAGCACAA+GGG 0.672388 1.4:+59543359 MS.gene40013:CDS
GTGATGGTGAGGGTAAGTGT+TGG 0.677988 1.4:+59543484 MS.gene40013:CDS
CTGGTTATCCAAACTCTGAG+CGG 0.682803 1.4:-59543337 None:intergenic
GGTATGCGTAATCACAAACT+CGG 0.683642 1.4:+59542172 MS.gene40013:CDS
CCGTCTGCTTCCAATTACGT+CGG 0.687927 1.4:+59542277 MS.gene40013:CDS
ATGGAGTGGACATACGAAAG+GGG 0.691126 1.4:+59542756 MS.gene40013:CDS
AGAACTCTCAAGTGTCACGA+AGG 0.693137 1.4:+59543537 MS.gene40013:CDS
CTAAACCGTCTTTAGCGTAG+GGG 0.694839 1.4:-59542144 None:intergenic
TACCATATTCCCATACCCTG+AGG 0.706671 1.4:-59543230 None:intergenic
GAAGGCATAGAATGCATGTG+TGG 0.724483 1.4:-59543282 None:intergenic
AACATAAGGAATCTTCCCAG+TGG 0.726027 1.4:-59543019 None:intergenic
AGATCAGAAGAAATACGCTG+AGG 0.737161 1.4:+59542546 MS.gene40013:CDS
TTGGTGTGAAGTGAAATGGA+AGG 0.740439 1.4:-59543300 None:intergenic
AGTATTGGGATACCGAAACG+GGG 0.752897 1.4:+59542977 MS.gene40013:CDS
AGAGTATGATCAGCATTTGG+GGG 0.760214 1.4:+59543143 MS.gene40013:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAAGAATATTAAGTAT+TGG + chr1.4:59542962-59542981 MS.gene40013:CDS 15.0%
!! TGATAAGAATATTAAGTATT+GGG + chr1.4:59542963-59542982 MS.gene40013:CDS 15.0%
!!! CATAACACCTTTTTAAAATT+CGG + chr1.4:59542241-59542260 MS.gene40013:CDS 20.0%
! AAATGTATGAGGACTTATAT+GGG + chr1.4:59542874-59542893 MS.gene40013:CDS 25.0%
! AGATACTTATTTATAGCACA+AGG + chr1.4:59543358-59543377 MS.gene40013:CDS 25.0%
! ATTGTTATCGACAAAAGTAA+TGG - chr1.4:59543178-59543197 None:intergenic 25.0%
! CTGATATACTTTATAACTAC+AGG - chr1.4:59543258-59543277 None:intergenic 25.0%
! GATACTTATTTATAGCACAA+GGG + chr1.4:59543359-59543378 MS.gene40013:CDS 25.0%
! TAAATGTATGAGGACTTATA+TGG + chr1.4:59542873-59542892 MS.gene40013:CDS 25.0%
! TTCAACTTAACAAAAAGAAG+AGG + chr1.4:59543388-59543407 MS.gene40013:CDS 25.0%
! TTGTGCTATAAATAAGTATC+TGG - chr1.4:59543359-59543378 None:intergenic 25.0%
!!! AAAGATAAGAGTACTTTTCA+TGG + chr1.4:59542631-59542650 MS.gene40013:CDS 25.0%
!!! AAGATAAGAGTACTTTTCAT+GGG + chr1.4:59542632-59542651 MS.gene40013:CDS 25.0%
!!! AGCATAACCGAATTTTAAAA+AGG - chr1.4:59542251-59542270 None:intergenic 25.0%
!!! GATATTTGTTTTACCAATGA+TGG + chr1.4:59542916-59542935 MS.gene40013:CDS 25.0%
!!! TTACTTTTGTCGATAACAAT+CGG + chr1.4:59543178-59543197 MS.gene40013:CDS 25.0%
AATGTATGAGGACTTATATG+GGG + chr1.4:59542875-59542894 MS.gene40013:CDS 30.0%
CAAAAACTTAGTTCCATCAT+TGG - chr1.4:59542932-59542951 None:intergenic 30.0%
GGATACTAAGGTTAAGATTT+GGG + chr1.4:59542834-59542853 MS.gene40013:CDS 30.0%
TCAATACTGGTAAATGTATG+AGG + chr1.4:59542863-59542882 MS.gene40013:CDS 30.0%
TGATAAGAAGATTGTTCAGT+GGG + chr1.4:59543095-59543114 MS.gene40013:CDS 30.0%
TGGATACTAAGGTTAAGATT+TGG + chr1.4:59542833-59542852 MS.gene40013:CDS 30.0%
! GGATAAACAGAATGTTTTGT+TGG + chr1.4:59543062-59543081 MS.gene40013:CDS 30.0%
! TTTTTCTCTTCTTCTGTTCA+CGG - chr1.4:59542357-59542376 None:intergenic 30.0%
!! ATTTGGGATGTTTTCAATAC+TGG + chr1.4:59542850-59542869 MS.gene40013:CDS 30.0%
!!! ATTAGGTTTTTTCCGAATTC+GGG + chr1.4:59542787-59542806 MS.gene40013:CDS 30.0%
!!! GATTAGGTTTTTTCCGAATT+CGG + chr1.4:59542786-59542805 MS.gene40013:CDS 30.0%
AAAGAGGAAAGGGATTATCA+AGG + chr1.4:59542655-59542674 MS.gene40013:CDS 35.0%
AACAAAAAGAAGAGGTTTGC+TGG + chr1.4:59543396-59543415 MS.gene40013:CDS 35.0%
AACAACAACGAAGATGAAGA+CGG + chr1.4:59542424-59542443 MS.gene40013:CDS 35.0%
ACATTCTGTTTATCCTCATC+TGG - chr1.4:59543057-59543076 None:intergenic 35.0%
AGAATCAAATGACCCGAATT+CGG - chr1.4:59542802-59542821 None:intergenic 35.0%
ATCTCAAAACCCTAATTCAC+CGG + chr1.4:59541940-59541959 MS.gene40013:CDS 35.0%
CATGTATGTACCAATCTCTT+CGG - chr1.4:59542739-59542758 None:intergenic 35.0%
CATTCTGTTTATCCTCATCT+GGG - chr1.4:59543056-59543075 None:intergenic 35.0%
CTGTTATATACCGAAGAGAT+TGG + chr1.4:59542726-59542745 MS.gene40013:CDS 35.0%
GAAGAATAAGAAGAGTCCTT+GGG + chr1.4:59542486-59542505 MS.gene40013:CDS 35.0%
GTGATAAGAAGATTGTTCAG+TGG + chr1.4:59543094-59543113 MS.gene40013:CDS 35.0%
TGAAGAATAAGAAGAGTCCT+TGG + chr1.4:59542485-59542504 MS.gene40013:CDS 35.0%
TTCAGTGGGATATGAATACT+GGG + chr1.4:59543109-59543128 MS.gene40013:CDS 35.0%
! AAGAGTATGATCAGCATTTG+GGG + chr1.4:59543142-59543161 MS.gene40013:CDS 35.0%
! CAAGAGTATGATCAGCATTT+GGG + chr1.4:59543141-59543160 MS.gene40013:CDS 35.0%
! GAGAATTTTCCGGTGAATTA+GGG - chr1.4:59541952-59541971 None:intergenic 35.0%
! TCAAGAGTATGATCAGCATT+TGG + chr1.4:59543140-59543159 MS.gene40013:CDS 35.0%
! TTTTCATGGGAAAGAGGAAA+GGG + chr1.4:59542645-59542664 MS.gene40013:CDS 35.0%
!! AAACAGAATGTTTTGTTGGC+TGG + chr1.4:59543066-59543085 MS.gene40013:CDS 35.0%
!! TAAGTATTGGGATACCGAAA+CGG + chr1.4:59542975-59542994 MS.gene40013:CDS 35.0%
!! TTATATCGACTTTCTCCACT+GGG + chr1.4:59543004-59543023 MS.gene40013:CDS 35.0%
!!! TTTTGGTGGTGAAAAAGTTG+AGG + chr1.4:59542603-59542622 MS.gene40013:CDS 35.0%
AACATAAGGAATCTTCCCAG+TGG - chr1.4:59543022-59543041 None:intergenic 40.0%
AGAGTATGATCAGCATTTGG+GGG + chr1.4:59543143-59543162 MS.gene40013:CDS 40.0%
AGATCAGAAGAAATACGCTG+AGG + chr1.4:59542546-59542565 MS.gene40013:CDS 40.0%
AGATTGGTACATACATGGAG+TGG + chr1.4:59542742-59542761 MS.gene40013:CDS 40.0%
AGGAAAGGGATTATCAAGGT+AGG + chr1.4:59542659-59542678 MS.gene40013:CDS 40.0%
ATCGAAATCACCGACGTAAT+TGG - chr1.4:59542290-59542309 None:intergenic 40.0%
CGAAGAGATTGGTACATACA+TGG + chr1.4:59542737-59542756 MS.gene40013:CDS 40.0%
GGGTTAAGCTTCACAACATA+AGG - chr1.4:59543036-59543055 None:intergenic 40.0%
GGTATGCGTAATCACAAACT+CGG + chr1.4:59542172-59542191 MS.gene40013:CDS 40.0%
GTTCAGTGGGATATGAATAC+TGG + chr1.4:59543108-59543127 MS.gene40013:CDS 40.0%
TGTTATGCTGTTCATCGAAG+AGG - chr1.4:59542228-59542247 None:intergenic 40.0%
TTGGTGTGAAGTGAAATGGA+AGG - chr1.4:59543303-59543322 None:intergenic 40.0%
TTTGATTCTGTCTGCTGGTA+TGG + chr1.4:59542813-59542832 MS.gene40013:CDS 40.0%
TTTGCTGGACATATTGTTGC+TGG + chr1.4:59543411-59543430 MS.gene40013:CDS 40.0%
! CTTTTCATGGGAAAGAGGAA+AGG + chr1.4:59542644-59542663 MS.gene40013:CDS 40.0%
! GAGTACTTTTCATGGGAAAG+AGG + chr1.4:59542639-59542658 MS.gene40013:CDS 40.0%
! GGAGAATTTTCCGGTGAATT+AGG - chr1.4:59541953-59541972 None:intergenic 40.0%
! TTACTCGCCTTAGCATCTTT+TGG - chr1.4:59542700-59542719 None:intergenic 40.0%
! TTGTCAGGTCAATTTTTCGC+CGG + chr1.4:59543440-59543459 MS.gene40013:CDS 40.0%
!! AAGTATTGGGATACCGAAAC+GGG + chr1.4:59542976-59542995 MS.gene40013:CDS 40.0%
!! GTTATATCGACTTTCTCCAC+TGG + chr1.4:59543003-59543022 MS.gene40013:CDS 40.0%
!!! GGAACTAAGTTTTTGAGTGC+TGG + chr1.4:59542937-59542956 MS.gene40013:CDS 40.0%
!!! GGTGAATTAGGGTTTTGAGA+TGG - chr1.4:59541941-59541960 None:intergenic 40.0%
AAGAAAGGCGAAGAGAAGAG+TGG + chr1.4:59542580-59542599 MS.gene40013:CDS 45.0%
AATAAGAAGAGTCCTTGGGC+TGG + chr1.4:59542490-59542509 MS.gene40013:CDS 45.0%
ACATGGAGTGGACATACGAA+AGG + chr1.4:59542754-59542773 MS.gene40013:CDS 45.0%
ACCATATTCCCATACCCTGA+GGG - chr1.4:59543232-59543251 None:intergenic 45.0%
AGAACTCTCAAGTGTCACGA+AGG + chr1.4:59543537-59543556 MS.gene40013:CDS 45.0%
ATAAGAAGAGTCCTTGGGCT+GGG + chr1.4:59542491-59542510 MS.gene40013:CDS 45.0%
ATGGAGTGGACATACGAAAG+GGG + chr1.4:59542756-59542775 MS.gene40013:CDS 45.0%
CACTTCACACCAATGCCAAT+TGG + chr1.4:59543310-59543329 MS.gene40013:CDS 45.0%
CATGGAGTGGACATACGAAA+GGG + chr1.4:59542755-59542774 MS.gene40013:CDS 45.0%
CTAAACCGTCTTTAGCGTAG+GGG - chr1.4:59542147-59542166 None:intergenic 45.0%
CTGGTTATCCAAACTCTGAG+CGG - chr1.4:59543340-59543359 None:intergenic 45.0%
GACATGACAAAACGTCCATC+CGG - chr1.4:59543462-59543481 None:intergenic 45.0%
GATTGAAAACCCAGCTTCTG+AGG + chr1.4:59542453-59542472 MS.gene40013:CDS 45.0%
GCTAAACCGTCTTTAGCGTA+GGG - chr1.4:59542148-59542167 None:intergenic 45.0%
GCTAAAGACGGTTTAGCACA+AGG + chr1.4:59542151-59542170 MS.gene40013:CDS 45.0%
GGGAAGAAGGAAGGTTTACA+AGG + chr1.4:59542511-59542530 MS.gene40013:CDS 45.0%
GGGATTATCAAGGTAGGTCT+TGG + chr1.4:59542665-59542684 MS.gene40013:CDS 45.0%
GGTCATTTGATTCTGTCTGC+TGG + chr1.4:59542808-59542827 MS.gene40013:CDS 45.0%
GTCTGCTGGTATGGATACTA+AGG + chr1.4:59542822-59542841 MS.gene40013:CDS 45.0%
TACCATATTCCCATACCCTG+AGG - chr1.4:59543233-59543252 None:intergenic 45.0%
TCTTCTTCTGTTCACGGTGT+GGG - chr1.4:59542351-59542370 None:intergenic 45.0%
TGCTAAACCGTCTTTAGCGT+AGG - chr1.4:59542149-59542168 None:intergenic 45.0%
TGCTGTTCATCGAAGAGGAA+AGG - chr1.4:59542223-59542242 None:intergenic 45.0%
TGTGAAGCTTAACCCAGATG+AGG + chr1.4:59543041-59543060 MS.gene40013:CDS 45.0%
TGTTGCTGGATATGCTTGTC+AGG + chr1.4:59543425-59543444 MS.gene40013:CDS 45.0%
! AGACGAGGTGGAGAATTTTC+CGG - chr1.4:59541962-59541981 None:intergenic 45.0%
! AGTATTGGGATACCGAAACG+GGG + chr1.4:59542977-59542996 MS.gene40013:CDS 45.0%
! GAAGGCATAGAATGCATGTG+TGG - chr1.4:59543285-59543304 None:intergenic 45.0%
! GATGGACGTTTTGTCATGTC+GGG + chr1.4:59543462-59543481 MS.gene40013:CDS 45.0%
! GGATGGACGTTTTGTCATGT+CGG + chr1.4:59543461-59543480 MS.gene40013:CDS 45.0%
!! AAGGCGATAAGGTGTTGAGT+TGG - chr1.4:59542076-59542095 None:intergenic 45.0%
!! AGGCGATAAGGTGTTGAGTT+GGG - chr1.4:59542075-59542094 None:intergenic 45.0%
!! GGCATTGGTGTGAAGTGAAA+TGG - chr1.4:59543307-59543326 None:intergenic 45.0%
!! GTAAGTGTTGGTTCTGGGAT+TGG + chr1.4:59543496-59543515 MS.gene40013:CDS 45.0%
!! TGAGGGTAAGTGTTGGTTCT+GGG + chr1.4:59543491-59543510 MS.gene40013:CDS 45.0%
!!! GAAGAGAAGAGTGGTTTTGG+TGG + chr1.4:59542589-59542608 MS.gene40013:CDS 45.0%
!!! GGTTTTGAGATGGTGAGTCT+GGG - chr1.4:59541931-59541950 None:intergenic 45.0%
AACAACCATGCCTCAGAAGC+TGG - chr1.4:59542466-59542485 None:intergenic 50.0%
ACAACCATGCCTCAGAAGCT+GGG - chr1.4:59542465-59542484 None:intergenic 50.0%
AGGTAGGGTTGAAGGCGATA+AGG - chr1.4:59542087-59542106 None:intergenic 50.0%
ATGACAAGTCCCTCAGGGTA+TGG + chr1.4:59543220-59543239 MS.gene40013:CDS 50.0%
CAGTGATGACAAGTCCCTCA+GGG + chr1.4:59543215-59543234 MS.gene40013:CDS 50.0%
CCGACGTAATTGGAAGCAGA+CGG - chr1.4:59542280-59542299 None:intergenic 50.0%
CCGTCTGCTTCCAATTACGT+CGG + chr1.4:59542277-59542296 MS.gene40013:CDS 50.0%
CGAAAGGGGTTTCTGCGATT+AGG + chr1.4:59542770-59542789 MS.gene40013:CDS 50.0%
CGATATAACCTGCCCCGTTT+CGG - chr1.4:59542992-59543011 None:intergenic 50.0%
CGTTGTTGTCGTCGGTGTAT+TGG - chr1.4:59541907-59541926 None:intergenic 50.0%
CTCTTCTTCTGTTCACGGTG+TGG - chr1.4:59542352-59542371 None:intergenic 50.0%
CTTTAGCGTAGGGGTGAGAT+GGG - chr1.4:59542138-59542157 None:intergenic 50.0%
GTATGATCAGCATTTGGGGG+CGG + chr1.4:59543146-59543165 MS.gene40013:CDS 50.0%
TCCCTCAGGGTATGGGAATA+TGG + chr1.4:59543228-59543247 MS.gene40013:CDS 50.0%
TCTCACCCCTACGCTAAAGA+CGG + chr1.4:59542139-59542158 MS.gene40013:CDS 50.0%
TCTTTAGCGTAGGGGTGAGA+TGG - chr1.4:59542139-59542158 None:intergenic 50.0%
TGACAAGTCCCTCAGGGTAT+GGG + chr1.4:59543221-59543240 MS.gene40013:CDS 50.0%
TGCGCCTCCAAAAGATGCTA+AGG + chr1.4:59542690-59542709 MS.gene40013:CDS 50.0%
! ACGGGTTTGGAGAGGGTTTT+GGG - chr1.4:59542049-59542068 None:intergenic 50.0%
! CAGGTCAATTTTTCGCCGGA+TGG + chr1.4:59543444-59543463 MS.gene40013:CDS 50.0%
! CGTTTTGTCATGTCGGGTGA+TGG + chr1.4:59543468-59543487 MS.gene40013:CDS 50.0%
! CTCGCCTTAGCATCTTTTGG+AGG - chr1.4:59542697-59542716 None:intergenic 50.0%
! GCTACTTTGCTCTGTTCCAG+AGG - chr1.4:59543588-59543607 None:intergenic 50.0%
! GGCGAAGAGAAGAGTGGTTT+TGG + chr1.4:59542586-59542605 MS.gene40013:CDS 50.0%
! GGGGCATTCGAAAGCTGTTA+GGG + chr1.4:59542894-59542913 MS.gene40013:CDS 50.0%
! GTGATGGTGAGGGTAAGTGT+TGG + chr1.4:59543484-59543503 MS.gene40013:CDS 50.0%
! TGGGGCATTCGAAAGCTGTT+AGG + chr1.4:59542893-59542912 MS.gene40013:CDS 50.0%
!! AAAACCCAGCTTCTGAGGCA+TGG + chr1.4:59542458-59542477 MS.gene40013:CDS 50.0%
!! AAGGTGTTGAGTTGGGTCGA+CGG - chr1.4:59542068-59542087 None:intergenic 50.0%
!! AGCATGGTGTCGTCGACTTT+GGG - chr1.4:59542007-59542026 None:intergenic 50.0%
!! GGGACGGGTTGATAAAGTAC+TGG + chr1.4:59543619-59543638 MS.gene40013:CDS 50.0%
!! GTGAGGGTAAGTGTTGGTTC+TGG + chr1.4:59543490-59543509 MS.gene40013:CDS 50.0%
!! TGTCACGAAGGCGTTACCAT+TGG + chr1.4:59543549-59543568 MS.gene40013:CDS 50.0%
!!! GGGTTTTGAGATGGTGAGTC+TGG - chr1.4:59541932-59541951 None:intergenic 50.0%
!!! TTTGGAGAGGGTTTTGGGGT+CGG - chr1.4:59542044-59542063 None:intergenic 50.0%
AGGATGCCACTCGCAACCAA+TGG - chr1.4:59543568-59543587 None:intergenic 55.0%
CAAAGTAGCGACGTGTGGCT+GGG + chr1.4:59543599-59543618 MS.gene40013:CDS 55.0%
CAGAGCAAAGTAGCGACGTG+TGG + chr1.4:59543594-59543613 MS.gene40013:CDS 55.0%
CCAGTGATGACAAGTCCCTC+AGG + chr1.4:59543214-59543233 MS.gene40013:CDS 55.0%
CCTGAGGGACTTGTCATCAC+TGG - chr1.4:59543217-59543236 None:intergenic 55.0%
CTGGTCGTAGGTAGGGTTGA+AGG - chr1.4:59542095-59542114 None:intergenic 55.0%
GGGTGAGATGGGCCTTGAAT+CGG - chr1.4:59542127-59542146 None:intergenic 55.0%
GGTGAGATGGGCCTTGAATC+GGG - chr1.4:59542126-59542145 None:intergenic 55.0%
GTCTGGGTCGTTGTTGTCGT+CGG - chr1.4:59541915-59541934 None:intergenic 55.0%
GTGAGATGGGCCTTGAATCG+GGG - chr1.4:59542125-59542144 None:intergenic 55.0%
! CGGGTTTGGAGAGGGTTTTG+GGG - chr1.4:59542048-59542067 None:intergenic 55.0%
! GAGGAGCACGCGAAGAAGAA+AGG + chr1.4:59542565-59542584 MS.gene40013:CDS 55.0%
! GCGTTACCATTGGTTGCGAG+TGG + chr1.4:59543559-59543578 MS.gene40013:CDS 55.0%
! GTCATGTCGGGTGATGGTGA+GGG + chr1.4:59543474-59543493 MS.gene40013:CDS 55.0%
! TGTCATGTCGGGTGATGGTG+AGG + chr1.4:59543473-59543492 MS.gene40013:CDS 55.0%
!! AGGTGTTGAGTTGGGTCGAC+GGG - chr1.4:59542067-59542086 None:intergenic 55.0%
!! CATGGTGTCGTCGACTTTGG+GGG - chr1.4:59542005-59542024 None:intergenic 55.0%
!! GACGGGTTTGGAGAGGGTTT+TGG - chr1.4:59542050-59542069 None:intergenic 55.0%
!! GAGCATGGTGTCGTCGACTT+TGG - chr1.4:59542008-59542027 None:intergenic 55.0%
!! GCATGGTGTCGTCGACTTTG+GGG - chr1.4:59542006-59542025 None:intergenic 55.0%
!! TTGAGTTGGGTCGACGGGTT+TGG - chr1.4:59542062-59542081 None:intergenic 55.0%
ACCCTACCTACGACCAGCTC+TGG + chr1.4:59542098-59542117 MS.gene40013:CDS 60.0%
ACTCTGAGCGGCGAGCCAAT+TGG - chr1.4:59543328-59543347 None:intergenic 60.0%
AGCGGCGAGCCAATTGGCAT+TGG - chr1.4:59543322-59543341 None:intergenic 60.0%
CCCAGAGCTGGTCGTAGGTA+GGG - chr1.4:59542102-59542121 None:intergenic 60.0%
CCCTACCTACGACCAGCTCT+GGG + chr1.4:59542099-59542118 MS.gene40013:CDS 60.0%
CCTTCCTTCTTCCCAGCCCA+AGG - chr1.4:59542505-59542524 None:intergenic 60.0%
GCAAAGTAGCGACGTGTGGC+TGG + chr1.4:59543598-59543617 MS.gene40013:CDS 60.0%
TGGTTGCGAGTGGCATCCTC+TGG + chr1.4:59543569-59543588 MS.gene40013:CDS 60.0%
TTGGGATACCGAAACGGGGC+AGG + chr1.4:59542981-59543000 MS.gene40013:CDS 60.0%
! CCTTGGGCTGGGAAGAAGGA+AGG + chr1.4:59542502-59542521 MS.gene40013:CDS 60.0%
! GAGTCCTTGGGCTGGGAAGA+AGG + chr1.4:59542498-59542517 MS.gene40013:CDS 60.0%
! TGGGTCGACGGGTTTGGAGA+GGG - chr1.4:59542056-59542075 None:intergenic 60.0%
! TTGGCTCGCCGCTCAGAGTT+TGG + chr1.4:59543329-59543348 MS.gene40013:CDS 60.0%
! TTGGGTCGACGGGTTTGGAG+AGG - chr1.4:59542057-59542076 None:intergenic 60.0%
! TTTGGGGTCGGCGACGGTTA+GGG - chr1.4:59542032-59542051 None:intergenic 60.0%
!!! TTTTGGGGTCGGCGACGGTT+AGG - chr1.4:59542033-59542052 None:intergenic 60.0%
CTTGAATCGGGGCCCAGAGC+TGG - chr1.4:59542114-59542133 None:intergenic 65.0%
GAACGGGAAGGGAGGAGACG+AGG - chr1.4:59541977-59541996 None:intergenic 65.0%
GCCCAGAGCTGGTCGTAGGT+AGG - chr1.4:59542103-59542122 None:intergenic 65.0%
GTAGCGACGTGTGGCTGGGA+CGG + chr1.4:59543603-59543622 MS.gene40013:CDS 65.0%
TAGCGACGTGTGGCTGGGAC+GGG + chr1.4:59543604-59543623 MS.gene40013:CDS 65.0%
!! CAGCTCTGGGCCCCGATTCA+AGG + chr1.4:59542112-59542131 MS.gene40013:CDS 65.0%
!! GGTGTCGTCGACTTTGGGGG+CGG - chr1.4:59542002-59542021 None:intergenic 65.0%
!!! GAGGGTTTTGGGGTCGGCGA+CGG - chr1.4:59542038-59542057 None:intergenic 65.0%
CGGGAAGGGAGGAGACGAGG+TGG - chr1.4:59541974-59541993 None:intergenic 70.0%
GGCGACGGTTAGGGCGAGCA+TGG - chr1.4:59542023-59542042 None:intergenic 70.0%
! CGACTTTGGGGGCGGCGGAA+CGG - chr1.4:59541994-59542013 None:intergenic 70.0%
! GACTTTGGGGGCGGCGGAAC+GGG - chr1.4:59541993-59542012 None:intergenic 70.0%
!! GTCGTCGACTTTGGGGGCGG+CGG - chr1.4:59541999-59542018 None:intergenic 70.0%
CGGGGCCCAGAGCTGGTCGT+AGG - chr1.4:59542107-59542126 None:intergenic 75.0%
TGGGGGCGGCGGAACGGGAA+GGG - chr1.4:59541988-59542007 None:intergenic 75.0%
TTGGGGGCGGCGGAACGGGA+AGG - chr1.4:59541989-59542008 None:intergenic 75.0%
! GGGCGGCGGAACGGGAAGGG+AGG - chr1.4:59541985-59542004 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 59541890 59543644 59541890 ID=MS.gene40013
chr1.4 mRNA 59541890 59543644 59541890 ID=MS.gene40013.t1;Parent=MS.gene40013
chr1.4 exon 59541890 59543644 59541890 ID=MS.gene40013.t1.exon1;Parent=MS.gene40013.t1
chr1.4 CDS 59541890 59543644 59541890 ID=cds.MS.gene40013.t1;Parent=MS.gene40013.t1
Gene Sequence

>MS.gene40013

ATGGATCTACTCAACCAATACACCGACGACAACAACGACCCAGACTCACCATCTCAAAACCCTAATTCACCGGAAAATTCTCCACCTCGTCTCCTCCCTTCCCGTTCCGCCGCCCCCAAAGTCGACGACACCATGCTCGCCCTAACCGTCGCCGACCCCAAAACCCTCTCCAAACCCGTCGACCCAACTCAACACCTTATCGCCTTCAACCCTACCTACGACCAGCTCTGGGCCCCGATTCAAGGCCCATCTCACCCCTACGCTAAAGACGGTTTAGCACAAGGTATGCGTAATCACAAACTCGGTTTCGTCGAAGACGCTAATATCGAACCTTTCCTCTTCGATGAACAGCATAACACCTTTTTAAAATTCGGTTATGCTGCTGATCCGTCTGCTTCCAATTACGTCGGTGATTTCGATGCTTTGCAGAAGAATAATGCTGCTTCTGTTTATAATATCCCACACCGTGAACAGAAGAAGAGAAAAATCGAATCTCTTAAGAAGAAAGAAGACAATGATAACGAAGAAGACAATAACAACAACGAAGATGAAGACGGTGTTGAGATTGAAAACCCAGCTTCTGAGGCATGGTTGTTGAAGAATAAGAAGAGTCCTTGGGCTGGGAAGAAGGAAGGTTTACAAGGAGAGTTAACTGAAGATCAGAAGAAATACGCTGAGGAGCACGCGAAGAAGAAAGGCGAAGAGAAGAGTGGTTTTGGTGGTGAAAAAGTTGAGGCTGTTAAAGATAAGAGTACTTTTCATGGGAAAGAGGAAAGGGATTATCAAGGTAGGTCTTGGATTGCGCCTCCAAAAGATGCTAAGGCGAGTAACGATCACTGTTATATACCGAAGAGATTGGTACATACATGGAGTGGACATACGAAAGGGGTTTCTGCGATTAGGTTTTTTCCGAATTCGGGTCATTTGATTCTGTCTGCTGGTATGGATACTAAGGTTAAGATTTGGGATGTTTTCAATACTGGTAAATGTATGAGGACTTATATGGGGCATTCGAAAGCTGTTAGGGATATTTGTTTTACCAATGATGGAACTAAGTTTTTGAGTGCTGGTTATGATAAGAATATTAAGTATTGGGATACCGAAACGGGGCAGGTTATATCGACTTTCTCCACTGGGAAGATTCCTTATGTTGTGAAGCTTAACCCAGATGAGGATAAACAGAATGTTTTGTTGGCTGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATACTGGGCAGATCACTCAAGAGTATGATCAGCATTTGGGGGCGGTGAATACCATTACTTTTGTCGATAACAATCGGAGATTTGTTACTTCCAGTGATGACAAGTCCCTCAGGGTATGGGAATATGGTATTCCTGTAGTTATAAAGTATATCAGTGAGCCACACATGCATTCTATGCCTTCCATTTCACTTCACACCAATGCCAATTGGCTCGCCGCTCAGAGTTTGGATAACCAGATACTTATTTATAGCACAAGGGAGAAGTTTCAACTTAACAAAAAGAAGAGGTTTGCTGGACATATTGTTGCTGGATATGCTTGTCAGGTCAATTTTTCGCCGGATGGACGTTTTGTCATGTCGGGTGATGGTGAGGGTAAGTGTTGGTTCTGGGATTGGAAGAGTTGCAAAGTCTTTAGAACTCTCAAGTGTCACGAAGGCGTTACCATTGGTTGCGAGTGGCATCCTCTGGAACAGAGCAAAGTAGCGACGTGTGGCTGGGACGGGTTGATAAAGTACTGGTAA
Protein sequence

>MS.gene40013.t1

MDLLNQYTDDNNDPDSPSQNPNSPENSPPRLLPSRSAAPKVDDTMLALTVADPKTLSKPVDPTQHLIAFNPTYDQLWAPIQGPSHPYAKDGLAQGMRNHKLGFVEDANIEPFLFDEQHNTFLKFGYAADPSASNYVGDFDALQKNNAASVYNIPHREQKKRKIESLKKKEDNDNEEDNNNNEDEDGVEIENPASEAWLLKNKKSPWAGKKEGLQGELTEDQKKYAEEHAKKKGEEKSGFGGEKVEAVKDKSTFHGKEERDYQGRSWIAPPKDAKASNDHCYIPKRLVHTWSGHTKGVSAIRFFPNSGHLILSAGMDTKVKIWDVFNTGKCMRTYMGHSKAVRDICFTNDGTKFLSAGYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDEDKQNVLLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEYGIPVVIKYISEPHMHSMPSISLHTNANWLAAQSLDNQILIYSTREKFQLNKKKRFAGHIVAGYACQVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHEGVTIGCEWHPLEQSKVATCGWDGLIKYW