Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40406.t1 | XP_013449734.1 | 97.5 | 363 | 8 | 1 | 1 | 363 | 1 | 362 | 2.10E-197 | 698.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40406.t1 | Q8H1F6 | 62.1 | 356 | 120 | 5 | 22 | 363 | 7 | 361 | 1.6e-121 | 437.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40406.t1 | A0A072U235 | 97.5 | 363 | 8 | 1 | 1 | 363 | 1 | 362 | 1.5e-197 | 698.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051582 | MS.gene40406 | 0.819308 | 1.24E-52 | -1.69E-46 |
| MS.gene052982 | MS.gene40406 | 0.80221 | 6.25E-49 | -1.69E-46 |
| MS.gene054917 | MS.gene40406 | 0.818453 | 1.95E-52 | -1.69E-46 |
| MS.gene056497 | MS.gene40406 | 0.827923 | 1.20E-54 | -1.69E-46 |
| MS.gene059247 | MS.gene40406 | 0.8058 | 1.12E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene40406 | 0.800789 | 1.22E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40406.t1 | MTR_7g094890 | 97.253 | 364 | 9 | 1 | 1 | 364 | 1 | 363 | 0.0 | 717 |
| MS.gene40406.t1 | MTR_7g091320 | 30.222 | 225 | 137 | 5 | 98 | 309 | 24 | 241 | 1.13e-23 | 100 |
| MS.gene40406.t1 | MTR_7g091320 | 30.222 | 225 | 137 | 5 | 98 | 309 | 24 | 241 | 3.44e-23 | 100 |
| MS.gene40406.t1 | MTR_4g081850 | 26.217 | 267 | 165 | 8 | 111 | 364 | 198 | 445 | 8.34e-16 | 79.7 |
| MS.gene40406.t1 | MTR_2g079510 | 25.830 | 271 | 161 | 8 | 111 | 364 | 206 | 453 | 1.27e-15 | 79.3 |
| MS.gene40406.t1 | MTR_7g094900 | 90.323 | 62 | 5 | 1 | 1 | 62 | 1 | 61 | 4.33e-15 | 77.8 |
| MS.gene40406.t1 | MTR_2g061470 | 27.103 | 214 | 135 | 6 | 92 | 292 | 23 | 228 | 1.47e-14 | 75.1 |
| MS.gene40406.t1 | MTR_1g048030 | 28.972 | 214 | 130 | 5 | 111 | 304 | 135 | 346 | 1.58e-14 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40406.t1 | AT4G02790 | 62.011 | 358 | 118 | 5 | 25 | 364 | 5 | 362 | 8.22e-158 | 450 |
| MS.gene40406.t1 | AT2G41670 | 31.718 | 227 | 131 | 5 | 98 | 309 | 26 | 243 | 5.31e-26 | 108 |
| MS.gene40406.t1 | AT3G07050 | 29.391 | 279 | 167 | 6 | 110 | 364 | 128 | 400 | 2.04e-19 | 90.9 |
| MS.gene40406.t1 | AT4G10650 | 28.571 | 210 | 128 | 7 | 99 | 290 | 27 | 232 | 5.12e-14 | 73.6 |
| MS.gene40406.t1 | AT1G52980 | 26.394 | 269 | 162 | 10 | 111 | 364 | 208 | 455 | 5.55e-14 | 74.3 |
Find 101 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTCAATGTTGTAGATTAT+TGG | 0.166115 | 7.2:+14720599 | MS.gene40406:intron |
| ATTGGTGGAGATAGAGAATT+TGG | 0.206923 | 7.2:-14720107 | None:intergenic |
| CATCATCATCATCTTGCTTA+TGG | 0.251466 | 7.2:+14720081 | MS.gene40406:CDS |
| ATGAAAGGTGGGTTCGATTT+GGG | 0.264909 | 7.2:+14723628 | MS.gene40406:intron |
| ACTCCTGGCCTTGGAGCTGC+TGG | 0.275454 | 7.2:-14722757 | None:intergenic |
| TCATCCCTTGCGGTTTGTTT+TGG | 0.284493 | 7.2:-14728830 | None:intergenic |
| GGTATTGGACAGTGGTTTCC+TGG | 0.286100 | 7.2:+14720799 | MS.gene40406:intron |
| TATGAAAGGTGGGTTCGATT+TGG | 0.308400 | 7.2:+14723627 | MS.gene40406:intron |
| AATTGTGGAGAAATTGAAAT+TGG | 0.308735 | 7.2:-14720125 | None:intergenic |
| GAGGGACTGGGACCGATCTT+TGG | 0.309126 | 7.2:+14720648 | MS.gene40406:CDS |
| TGGGAAAGATCTTGAGTTGC+TGG | 0.317900 | 7.2:+14723647 | MS.gene40406:CDS |
| ATCTCAATCTCCCACCATTC+AGG | 0.333290 | 7.2:+14720208 | MS.gene40406:CDS |
| AGGAAGAAAGAAACAGAAAA+AGG | 0.341120 | 7.2:+14728911 | MS.gene40406:CDS |
| CTTGAGTTGCTGGACTCTCC+TGG | 0.351077 | 7.2:+14723657 | MS.gene40406:CDS |
| CTTGCGCTTCATTTGTTCAA+TGG | 0.362304 | 7.2:+14728223 | MS.gene40406:CDS |
| CTGCTAATTCCTTTGCTAAC+CGG | 0.374309 | 7.2:-14721897 | None:intergenic |
| GTTGATGGATGTTGTTATAG+AGG | 0.386526 | 7.2:+14720864 | MS.gene40406:CDS |
| GTAACAACCACATGCACTAT+AGG | 0.403608 | 7.2:+14728804 | MS.gene40406:CDS |
| ATTACAAACAAGGGCAAAAC+AGG | 0.406881 | 7.2:+14728891 | MS.gene40406:CDS |
| GTCTTCTCCAATGGGCAGCT+TGG | 0.417848 | 7.2:+14721762 | MS.gene40406:CDS |
| CAAGGAACAACTTAAGTTGA+TGG | 0.420000 | 7.2:+14720849 | MS.gene40406:CDS |
| CTCCAATGGGCAGCTTGGAA+TGG | 0.425085 | 7.2:+14721767 | MS.gene40406:CDS |
| GGAGATTGAGATTGGAGGGT+TGG | 0.441330 | 7.2:-14720197 | None:intergenic |
| TAGGTTCGAGCTGGAATAGT+TGG | 0.444220 | 7.2:+14722676 | MS.gene40406:intron |
| GATGGTTCCGAAGGAGGGAC+TGG | 0.447105 | 7.2:+14720635 | MS.gene40406:CDS |
| CTCTCCAATGTCATCACATA+TGG | 0.452328 | 7.2:-14723727 | None:intergenic |
| CAGCTGATGTAAATGTTAAA+CGG | 0.455115 | 7.2:+14721917 | MS.gene40406:CDS |
| ATCGGTCCCAGTCCCTCCTT+CGG | 0.468176 | 7.2:-14720642 | None:intergenic |
| TGCCCAGCAGCTCCAAGGCC+AGG | 0.470383 | 7.2:+14722754 | MS.gene40406:CDS |
| AACTGCAGATCGAAATGCTT+GGG | 0.470581 | 7.2:+14721707 | MS.gene40406:CDS |
| CTCAAATCTCTTGTAACTCC+TGG | 0.470935 | 7.2:-14722772 | None:intergenic |
| ACATTCTTCTCTTTAACAAA+CGG | 0.471895 | 7.2:-14722732 | None:intergenic |
| TTATTCCCACCCGCAGTAGC+AGG | 0.476643 | 7.2:+14720147 | MS.gene40406:CDS |
| TCATATTGCAAAAGCAGAAA+AGG | 0.487875 | 7.2:+14720822 | MS.gene40406:CDS |
| CAAAGGACTACTTCCTCGTC+CGG | 0.491033 | 7.2:+14721945 | MS.gene40406:CDS |
| ACAAAGGTTGTCTTCTCCAA+TGG | 0.491090 | 7.2:+14721753 | MS.gene40406:CDS |
| CTCCTGGCCTTGGAGCTGCT+GGG | 0.493561 | 7.2:-14722756 | None:intergenic |
| CGGACGAGGAAGTAGTCCTT+TGG | 0.500629 | 7.2:-14721944 | None:intergenic |
| GAATGGTGGGAGATTGAGAT+TGG | 0.500709 | 7.2:-14720205 | None:intergenic |
| AACCATTCCAAGCTGCCCAT+TGG | 0.512603 | 7.2:-14721769 | None:intergenic |
| TTGACACTGATCTTTATCAC+TGG | 0.513007 | 7.2:+14720678 | MS.gene40406:CDS |
| CAAAGGTTGTCTTCTCCAAT+GGG | 0.513498 | 7.2:+14721754 | MS.gene40406:CDS |
| TTGAGTTGCTGGACTCTCCT+GGG | 0.515009 | 7.2:+14723658 | MS.gene40406:CDS |
| CAGGAGTTACAAGAGATTTG+AGG | 0.515630 | 7.2:+14722773 | MS.gene40406:CDS |
| TGCAATCATGTAATCGAGTA+CGG | 0.519885 | 7.2:-14728868 | None:intergenic |
| TCTCTTGTAACTCCTGGCCT+TGG | 0.519921 | 7.2:-14722766 | None:intergenic |
| TTGTAGATGGATCTATGGCT+TGG | 0.521384 | 7.2:+14721639 | MS.gene40406:intron |
| AGGTTCGAGCTGGAATAGTT+GGG | 0.525535 | 7.2:+14722677 | MS.gene40406:intron |
| GACTGGTCACTGATTCGCAT+TGG | 0.525801 | 7.2:-14723687 | None:intergenic |
| ATGGTTCCGAAGGAGGGACT+GGG | 0.528733 | 7.2:+14720636 | MS.gene40406:CDS |
| CATGATTGCAATTACAAACA+AGG | 0.529477 | 7.2:+14728881 | MS.gene40406:CDS |
| GGAGAACCTGCTACTGCGGG+TGG | 0.532796 | 7.2:-14720153 | None:intergenic |
| TGTGGAGAAATTGAAATTGG+TGG | 0.533927 | 7.2:-14720122 | None:intergenic |
| ATTGGCAACAACACATTCGA+TGG | 0.539468 | 7.2:+14720617 | MS.gene40406:CDS |
| TTGTTATAGAGGTACGAGAC+GGG | 0.546757 | 7.2:+14720875 | MS.gene40406:CDS |
| TGGTGGGAGATTGAGATTGG+AGG | 0.546785 | 7.2:-14720202 | None:intergenic |
| GTCCTGTCAGACTTTAGAAA+AGG | 0.552851 | 7.2:+14728271 | MS.gene40406:CDS |
| TGATGATACATACCTACTGT+TGG | 0.554845 | 7.2:-14723807 | None:intergenic |
| CTGCGGGTGGGAATAAATTG+TGG | 0.555305 | 7.2:-14720140 | None:intergenic |
| AAGTGGAGAACCTGCTACTG+CGG | 0.555716 | 7.2:-14720157 | None:intergenic |
| AATAGTTGGGTACCCTAATG+TGG | 0.558464 | 7.2:+14722690 | MS.gene40406:CDS |
| ACTTACCTGTGGATGACTTG+TGG | 0.559160 | 7.2:-14720911 | None:intergenic |
| ATTCGCATTGGAAGTATCCC+AGG | 0.560318 | 7.2:-14723675 | None:intergenic |
| GGTGGGAGATTGAGATTGGA+GGG | 0.562781 | 7.2:-14720201 | None:intergenic |
| CAACTGCAGATCGAAATGCT+TGG | 0.565332 | 7.2:+14721706 | MS.gene40406:CDS |
| TTCTAAAGTCTGACAGGACA+CGG | 0.568460 | 7.2:-14728267 | None:intergenic |
| CACATACTAAATACCTGCAC+AGG | 0.571976 | 7.2:-14720716 | None:intergenic |
| TAGAAGCAACCTACCGGACG+AGG | 0.573378 | 7.2:-14721958 | None:intergenic |
| AGGGTTGGTGTGGCAGCGAG+AGG | 0.574193 | 7.2:-14720182 | None:intergenic |
| GTCAAGACCAGTCCAAAGAT+CGG | 0.574666 | 7.2:-14720660 | None:intergenic |
| CATTCGATGGTTCCGAAGGA+GGG | 0.577239 | 7.2:+14720630 | MS.gene40406:CDS |
| ACTATTGTATAGGTTCGAGC+TGG | 0.578842 | 7.2:+14722667 | MS.gene40406:intron |
| TTGGTTTCCTATAGTGCATG+TGG | 0.579634 | 7.2:-14728811 | None:intergenic |
| ATGATTGCAATTACAAACAA+GGG | 0.583457 | 7.2:+14728882 | MS.gene40406:CDS |
| TTGAGATTGGAGGGTTGGTG+TGG | 0.584151 | 7.2:-14720192 | None:intergenic |
| AGTGGAGAACCTGCTACTGC+GGG | 0.585138 | 7.2:-14720156 | None:intergenic |
| AAAAGCAGAAAAGGAACTCA+AGG | 0.594084 | 7.2:+14720831 | MS.gene40406:CDS |
| GAATGTGCCCAGCAGCTCCA+AGG | 0.594202 | 7.2:+14722749 | MS.gene40406:CDS |
| GATGAAACTTTCATCCCTTG+CGG | 0.596220 | 7.2:-14728840 | None:intergenic |
| AGGGCACATTGAAGCTAGGC+CGG | 0.597091 | 7.2:+14721878 | MS.gene40406:intron |
| CTTACAAAACTTCCAACAGT+AGG | 0.597306 | 7.2:+14723795 | MS.gene40406:CDS |
| AATGTTAAACGGAGAGCCAA+AGG | 0.598690 | 7.2:+14721928 | MS.gene40406:CDS |
| GTACAGGGCACATTGAAGCT+AGG | 0.604959 | 7.2:+14721874 | MS.gene40406:intron |
| GTTGTCCACAAGTCATCCAC+AGG | 0.607084 | 7.2:+14720906 | MS.gene40406:CDS |
| ACATTCGATGGTTCCGAAGG+AGG | 0.607094 | 7.2:+14720629 | MS.gene40406:CDS |
| AACACATTCGATGGTTCCGA+AGG | 0.607700 | 7.2:+14720626 | MS.gene40406:CDS |
| GAAGCTAGGCCGGTTAGCAA+AGG | 0.608408 | 7.2:+14721888 | MS.gene40406:CDS |
| ACTGGGACCGATCTTTGGAC+TGG | 0.609653 | 7.2:+14720653 | MS.gene40406:CDS |
| TGATCTTTATCACTGGACAA+AGG | 0.621272 | 7.2:+14720685 | MS.gene40406:CDS |
| GGAAACCAAAACAAACCGCA+AGG | 0.630708 | 7.2:+14728825 | MS.gene40406:CDS |
| CTTGCCATATGTGATGACAT+TGG | 0.632886 | 7.2:+14723723 | MS.gene40406:CDS |
| TTTCGTGTAGACAGTGAATG+TGG | 0.640512 | 7.2:+14723960 | MS.gene40406:CDS |
| ATAGTTGGGTACCCTAATGT+GGG | 0.643817 | 7.2:+14722691 | MS.gene40406:CDS |
| TGTGGCAGCGAGAGGTGAAG+TGG | 0.645729 | 7.2:-14720174 | None:intergenic |
| GAGAACCTGCTACTGCGGGT+GGG | 0.654778 | 7.2:-14720152 | None:intergenic |
| GAAACCAAAACAAACCGCAA+GGG | 0.680921 | 7.2:+14728826 | MS.gene40406:CDS |
| TGGAGAGAGGTCTTATGATG+TGG | 0.703310 | 7.2:+14723743 | MS.gene40406:CDS |
| TTATAGAGGTACGAGACGGG+CGG | 0.703873 | 7.2:+14720878 | MS.gene40406:CDS |
| TATGTGATGACATTGGAGAG+AGG | 0.708423 | 7.2:+14723730 | MS.gene40406:CDS |
| TGTGGATGACTTGTGGACAA+CGG | 0.717355 | 7.2:-14720904 | None:intergenic |
| GTTGTTATAGAGGTACGAGA+CGG | 0.721407 | 7.2:+14720874 | MS.gene40406:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 14720044 | 14728952 | 14720044 | ID=MS.gene40406 |
| chr7.2 | mRNA | 14720044 | 14728952 | 14720044 | ID=MS.gene40406.t1;Parent=MS.gene40406 |
| chr7.2 | exon | 14720044 | 14720229 | 14720044 | ID=MS.gene40406.t1.exon1;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14720044 | 14720229 | 14720044 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14720614 | 14720724 | 14720614 | ID=MS.gene40406.t1.exon2;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14720614 | 14720724 | 14720614 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14720811 | 14720927 | 14720811 | ID=MS.gene40406.t1.exon3;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14720811 | 14720927 | 14720811 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14721645 | 14721788 | 14721645 | ID=MS.gene40406.t1.exon4;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14721645 | 14721788 | 14721645 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14721880 | 14721966 | 14721880 | ID=MS.gene40406.t1.exon5;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14721880 | 14721966 | 14721880 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14722679 | 14722794 | 14722679 | ID=MS.gene40406.t1.exon6;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14722679 | 14722794 | 14722679 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14723635 | 14723816 | 14723635 | ID=MS.gene40406.t1.exon7;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14723635 | 14723816 | 14723635 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14723931 | 14723988 | 14723931 | ID=MS.gene40406.t1.exon8;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14723931 | 14723988 | 14723931 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14728210 | 14728292 | 14728210 | ID=MS.gene40406.t1.exon9;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14728210 | 14728292 | 14728210 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| chr7.2 | exon | 14728795 | 14728952 | 14728795 | ID=MS.gene40406.t1.exon10;Parent=MS.gene40406.t1 |
| chr7.2 | CDS | 14728795 | 14728952 | 14728795 | ID=cds.MS.gene40406.t1;Parent=MS.gene40406.t1 |
| Gene Sequence |
| Protein sequence |
