AlfalfaGEDB | MS.gene41161

M. sativa cultivar XinJiangDaYe / MS.gene41161

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41161.t1	RHN60916.1	96	150	4	1	1	150	1	148	1.90E-68	268.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41161.t1	Q1S053	94.1	152	7	1	1	150	1	152	2.3e-70	266.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41161.t1	A0A396I5J8	96.0	150	4	1	1	150	1	148	1.4e-68	268.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051263	MS.gene41161	0.837081	6.41E-57	-1.69E-46
MS.gene055957	MS.gene41161	0.847757	9.45E-60	-1.69E-46
MS.gene057458	MS.gene41161	0.809632	1.72E-50	-1.69E-46
MS.gene058844	MS.gene41161	0.820377	7.08E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41161.t1	MTR_2g096610	88.667	150	15	1	1	150	1	148	8.80e-84	243
MS.gene41161.t1	MTR_4g071150	94.079	152	7	1	1	150	1	152	6.08e-83	241
MS.gene41161.t1	MTR_4g063280	93.939	132	7	1	20	150	14	145	6.83e-82	238
MS.gene41161.t1	MTR_4g064967	94.667	150	6	1	1	150	1	148	1.25e-81	237
MS.gene41161.t1	MTR_4g064005	94.000	150	7	1	1	150	1	148	2.14e-81	236
MS.gene41161.t1	MTR_4g063410	92.667	150	9	1	1	150	1	148	9.20e-81	235
MS.gene41161.t1	MTR_2g096570	89.610	154	11	2	1	150	1	153	1.94e-79	232
MS.gene41161.t1	MTR_8g086640	96.970	132	4	0	1	132	1	132	3.54e-75	221
MS.gene41161.t1	MTR_7g113160	80.451	133	25	1	10	142	13	144	1.87e-71	211
MS.gene41161.t1	MTR_7g108320	72.093	129	36	0	4	132	2	130	1.72e-59	181
MS.gene41161.t1	MTR_7g114060	73.077	130	31	1	3	132	2	127	2.73e-59	180
MS.gene41161.t1	MTR_7g114040	73.077	130	31	1	3	132	2	127	2.73e-59	180
MS.gene41161.t1	MTR_7g093910	73.438	128	31	2	3	129	2	127	5.62e-59	179
MS.gene41161.t1	MTR_2g082510	74.790	119	30	0	11	129	3	121	1.33e-58	178
MS.gene41161.t1	MTR_2g082220	73.950	119	31	0	11	129	3	121	1.31e-57	176
MS.gene41161.t1	MTR_6g005150	63.780	127	32	3	24	149	26	139	3.72e-47	149
MS.gene41161.t1	MTR_7g114050	68.333	120	23	3	17	132	10	118	1.16e-45	145
MS.gene41161.t1	MTR_4g063300	75.238	105	21	1	45	149	10	109	1.51e-45	144
MS.gene41161.t1	MTR_1g011750	59.292	113	44	2	21	132	23	134	8.77e-36	120
MS.gene41161.t1	MTR_1g011850	59.292	113	44	2	21	132	23	134	8.77e-36	120
MS.gene41161.t1	MTR_1g114050	59.633	109	42	2	21	128	20	127	7.17e-35	118
MS.gene41161.t1	MTR_1g071040	61.538	104	38	2	26	128	28	130	4.33e-34	116
MS.gene41161.t1	MTR_1g069770	50.000	96	47	1	45	139	9	104	8.99e-26	94.4
MS.gene41161.t1	MTR_7g010440	70.588	51	15	0	78	128	122	172	1.24e-16	75.1
MS.gene41161.t1	MTR_4g005910	74.419	43	11	0	87	129	4	46	2.02e-16	69.3
MS.gene41161.t1	MTR_4g048490	43.810	105	30	2	24	128	26	101	3.89e-16	69.7
MS.gene41161.t1	MTR_7g027610	41.818	110	38	3	20	128	10	94	3.91e-15	67.4
MS.gene41161.t1	MTR_2g096590	75.000	40	10	0	110	149	12	51	7.74e-13	59.7
MS.gene41161.t1	MTR_0653s0030	53.704	54	25	0	56	109	44	97	1.34e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41161.t1	AT5G02560	81.699	153	25	1	1	150	1	153	1.05e-78	230
MS.gene41161.t1	AT5G02560	70.621	177	25	2	1	150	1	177	8.94e-74	218
MS.gene41161.t1	AT5G27670	75.000	152	34	3	1	150	1	150	1.11e-72	214
MS.gene41161.t1	AT5G59870	74.324	148	37	1	4	150	3	150	3.10e-71	211
MS.gene41161.t1	AT1G51060	72.093	129	36	0	11	139	3	131	1.53e-61	186
MS.gene41161.t1	AT4G27230	70.543	129	38	0	11	139	3	131	1.91e-60	183
MS.gene41161.t1	AT4G27230	70.543	129	38	0	11	139	3	131	1.91e-60	183
MS.gene41161.t1	AT3G20670	71.318	129	37	0	11	139	3	131	4.43e-60	182
MS.gene41161.t1	AT5G54640	72.000	125	35	0	11	135	3	127	1.24e-58	178
MS.gene41161.t1	AT1G08880	72.800	125	31	1	11	132	6	130	4.09e-58	177
MS.gene41161.t1	AT1G54690	72.800	125	31	1	11	132	6	130	6.40e-58	177
MS.gene41161.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.05e-36	122
MS.gene41161.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.05e-36	122
MS.gene41161.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.05e-36	122
MS.gene41161.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.05e-36	122
MS.gene41161.t1	AT1G52740	59.633	109	42	2	25	132	27	134	1.38e-34	117
MS.gene41161.t1	AT3G54560	58.716	109	43	2	25	132	29	136	2.08e-34	117
MS.gene41161.t1	AT3G54560	58.716	109	43	2	25	132	29	136	2.08e-34	117
MS.gene41161.t1	AT4G13570	47.826	92	46	2	32	122	28	118	5.87e-20	80.1
MS.gene41161.t1	AT4G13570	47.253	91	46	2	33	122	35	124	1.61e-19	79.0

Find 45 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAATCTAAGCCTTCTT+TGG	0.244387	4.1:+36412766	None:intergenic
CTTGTCACCGACTTCTTCCT+TGG	0.333455	4.1:+36413147	None:intergenic
GGCTCCTTTCTTCGCGGTCT+TGG	0.357028	4.1:+36413191	None:intergenic
AGAACTGCTGCTAGGTATAC+TGG	0.360588	4.1:+36413033	None:intergenic
AGCCTTCTTTGGCTCTTTGT+TGG	0.377450	4.1:+36412798	None:intergenic
CAATTTCCAGTTGGAAGAAT+CGG	0.397925	4.1:-36413105	MS.gene41161:CDS
TTCTTTGGCTCTTTGTTGGC+GGG	0.419461	4.1:+36412802	None:intergenic
CTTCTTTGGCTCTTTGTTGG+CGG	0.420001	4.1:+36412801	None:intergenic
TACGCTCAGCGTGTTGGAAC+CGG	0.422732	4.1:-36413060	MS.gene41161:CDS
TTTCAGTCTTCTTAGGCAAA+AGG	0.434052	4.1:+36412833	None:intergenic
TGCTGCTGAAGTTCTTGAGT+TGG	0.438702	4.1:-36413001	MS.gene41161:CDS
GGCATGTGTTGTTGGCTGTT+AGG	0.442957	4.1:-36412933	MS.gene41161:CDS
AACAAAGAGCCAAAGAAGGC+TGG	0.443832	4.1:-36412796	MS.gene41161:CDS
AAAAGGATAGGGTTAATGTT+TGG	0.446113	4.1:+36412850	None:intergenic
TCCTCCTCCTTTCCTTCCAC+CGG	0.452471	4.1:+36413170	None:intergenic
GAGCTTGGAAAATTACTTGC+TGG	0.470462	4.1:-36412901	MS.gene41161:CDS
TATTCCAAGGCATGTGTTGT+TGG	0.470522	4.1:-36412941	MS.gene41161:CDS
GCTGAAGTTCTTGAGTTGGC+TGG	0.471187	4.1:-36412997	MS.gene41161:CDS
AAAGGAGCCGGTGGAAGGAA+AGG	0.476592	4.1:-36413177	MS.gene41161:CDS
GTCTTCTTAGGCAAAAGGAT+AGG	0.482161	4.1:+36412838	None:intergenic
TATCGACCGATTCTTCCAAC+TGG	0.484363	4.1:+36413099	None:intergenic
GCCGGTGGAAGGAAAGGAGG+AGG	0.487329	4.1:-36413171	MS.gene41161:CDS
TCTTCTTAGGCAAAAGGATA+GGG	0.501511	4.1:+36412839	None:intergenic
AGTACCAAGACCGCGAAGAA+AGG	0.509768	4.1:-36413195	MS.gene41161:CDS
ACAGCCAACAACACATGCCT+TGG	0.515073	4.1:+36412937	None:intergenic
GTTAGGAACGATGAAGAGCT+TGG	0.516291	4.1:-36412916	MS.gene41161:CDS
ATCGGTCGATACTTGAAGAA+AGG	0.519021	4.1:-36413087	MS.gene41161:CDS
TTTGCCTAAGAAGACTGAAA+AGG	0.521479	4.1:-36412830	MS.gene41161:CDS
GGCTGGAAAATCTCCAAAGA+AGG	0.523644	4.1:-36412779	MS.gene41161:CDS
GGAAGATACGCTCAGCGTGT+TGG	0.531317	4.1:-36413066	MS.gene41161:CDS
TCCACCGGCTCCTTTCTTCG+CGG	0.538173	4.1:+36413185	None:intergenic
CGCCAACAAAGAGCCAAAGA+AGG	0.546195	4.1:-36412800	MS.gene41161:CDS
CGAAGAAAGGAGCCGGTGGA+AGG	0.552463	4.1:-36413182	MS.gene41161:CDS
GAAGGAAAGGAGGAGGACCA+AGG	0.555100	4.1:-36413164	MS.gene41161:CDS
GCTGGTGTCACAATTGCTCA+TGG	0.556477	4.1:-36412883	MS.gene41161:CDS
GGAGCCGGTGGAAGGAAAGG+AGG	0.561766	4.1:-36413174	MS.gene41161:CDS
GTGACAAGATCAATCAGAGC+TGG	0.586931	4.1:-36413132	MS.gene41161:CDS
GATATTCAAGAACTGCTGCT+AGG	0.592671	4.1:+36413025	None:intergenic
GCTGGTCTTCAATTTCCAGT+TGG	0.598252	4.1:-36413114	MS.gene41161:CDS
ACCGCGAAGAAAGGAGCCGG+TGG	0.603380	4.1:-36413186	MS.gene41161:CDS
TGCTAGGTATACTGGAGCAC+CGG	0.608337	4.1:+36413041	None:intergenic
AGGAGGACCAAGGAAGAAGT+CGG	0.610829	4.1:-36413154	MS.gene41161:CDS
AGAAGAATAGAATTATTCCA+AGG	0.617592	4.1:-36412954	MS.gene41161:CDS
AAGACCGCGAAGAAAGGAGC+CGG	0.634185	4.1:-36413189	MS.gene41161:CDS
GGTGTCACAATTGCTCATGG+TGG	0.701079	4.1:-36412880	MS.gene41161:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAGAATAGAATTATTCCA+AGG	-	chr4.1:36413024-36413043	MS.gene41161:CDS	25.0%
	AAAAGGATAGGGTTAATGTT+TGG	+	chr4.1:36413131-36413150	None:intergenic	30.0%
	CAATTTCCAGTTGGAAGAAT+CGG	-	chr4.1:36412873-36412892	MS.gene41161:CDS	35.0%
	TCTTCTTAGGCAAAAGGATA+GGG	+	chr4.1:36413142-36413161	None:intergenic	35.0%
	TTTCAGTCTTCTTAGGCAAA+AGG	+	chr4.1:36413148-36413167	None:intergenic	35.0%
	TTTGCCTAAGAAGACTGAAA+AGG	-	chr4.1:36413148-36413167	MS.gene41161:CDS	35.0%
	ATCGGTCGATACTTGAAGAA+AGG	-	chr4.1:36412891-36412910	MS.gene41161:CDS	40.0%
	GAGCTTGGAAAATTACTTGC+TGG	-	chr4.1:36413077-36413096	MS.gene41161:CDS	40.0%
	GATATTCAAGAACTGCTGCT+AGG	+	chr4.1:36412956-36412975	None:intergenic	40.0%
	GTCTTCTTAGGCAAAAGGAT+AGG	+	chr4.1:36413143-36413162	None:intergenic	40.0%
	TATTCCAAGGCATGTGTTGT+TGG	-	chr4.1:36413037-36413056	MS.gene41161:CDS	40.0%
	AACAAAGAGCCAAAGAAGGC+TGG	-	chr4.1:36413182-36413201	MS.gene41161:CDS	45.0%
	AGAACTGCTGCTAGGTATAC+TGG	+	chr4.1:36412948-36412967	None:intergenic	45.0%
	GGCTGGAAAATCTCCAAAGA+AGG	-	chr4.1:36413199-36413218	MS.gene41161:CDS	45.0%
	GTGACAAGATCAATCAGAGC+TGG	-	chr4.1:36412846-36412865	MS.gene41161:CDS	45.0%
	GTTAGGAACGATGAAGAGCT+TGG	-	chr4.1:36413062-36413081	MS.gene41161:CDS	45.0%
	TGCTGCTGAAGTTCTTGAGT+TGG	-	chr4.1:36412977-36412996	MS.gene41161:CDS	45.0%
!	GCAGCCTTTTCAGTCTTCTT+AGG	+	chr4.1:36413155-36413174	None:intergenic	45.0%
!	GGAGATTTTCCAGCCTTCTT+TGG	+	chr4.1:36413194-36413213	None:intergenic	45.0%
!	TATCGACCGATTCTTCCAAC+TGG	+	chr4.1:36412882-36412901	None:intergenic	45.0%
!!	AGCCTTCTTTGGCTCTTTGT+TGG	+	chr4.1:36413183-36413202	None:intergenic	45.0%
!!	CTTCTTTGGCTCTTTGTTGG+CGG	+	chr4.1:36413180-36413199	None:intergenic	45.0%
!!	GCTGGTCTTCAATTTCCAGT+TGG	-	chr4.1:36412864-36412883	MS.gene41161:CDS	45.0%
!!	TTCTTTGGCTCTTTGTTGGC+GGG	+	chr4.1:36413179-36413198	None:intergenic	45.0%
	ACAGCCAACAACACATGCCT+TGG	+	chr4.1:36413044-36413063	None:intergenic	50.0%
	AGGAGGACCAAGGAAGAAGT+CGG	-	chr4.1:36412824-36412843	MS.gene41161:CDS	50.0%
	AGTACCAAGACCGCGAAGAA+AGG	-	chr4.1:36412783-36412802	MS.gene41161:CDS	50.0%
	CGCCAACAAAGAGCCAAAGA+AGG	-	chr4.1:36413178-36413197	MS.gene41161:CDS	50.0%
	TGCTAGGTATACTGGAGCAC+CGG	+	chr4.1:36412940-36412959	None:intergenic	50.0%
!	GGCATGTGTTGTTGGCTGTT+AGG	-	chr4.1:36413045-36413064	MS.gene41161:CDS	50.0%
!	GGTGTCACAATTGCTCATGG+TGG	-	chr4.1:36413098-36413117	MS.gene41161:CDS	50.0%
!!	CTTGTCACCGACTTCTTCCT+TGG	+	chr4.1:36412834-36412853	None:intergenic	50.0%
!!	GCTGAAGTTCTTGAGTTGGC+TGG	-	chr4.1:36412981-36413000	MS.gene41161:CDS	50.0%
!!	GCTGGTGTCACAATTGCTCA+TGG	-	chr4.1:36413095-36413114	MS.gene41161:CDS	50.0%
	AAAGGAGCCGGTGGAAGGAA+AGG	-	chr4.1:36412801-36412820	MS.gene41161:CDS	55.0%
	AAGACCGCGAAGAAAGGAGC+CGG	-	chr4.1:36412789-36412808	MS.gene41161:CDS	55.0%
	GAAGGAAAGGAGGAGGACCA+AGG	-	chr4.1:36412814-36412833	MS.gene41161:CDS	55.0%
	GGAAGATACGCTCAGCGTGT+TGG	-	chr4.1:36412912-36412931	MS.gene41161:CDS	55.0%
	TACGCTCAGCGTGTTGGAAC+CGG	-	chr4.1:36412918-36412937	MS.gene41161:CDS	55.0%
	TCCTCCTCCTTTCCTTCCAC+CGG	+	chr4.1:36412811-36412830	None:intergenic	55.0%
	CGAAGAAAGGAGCCGGTGGA+AGG	-	chr4.1:36412796-36412815	MS.gene41161:CDS	60.0%
	GGCTCCTTTCTTCGCGGTCT+TGG	+	chr4.1:36412790-36412809	None:intergenic	60.0%
!!	TCCACCGGCTCCTTTCTTCG+CGG	+	chr4.1:36412796-36412815	None:intergenic	60.0%
	ACCGCGAAGAAAGGAGCCGG+TGG	-	chr4.1:36412792-36412811	MS.gene41161:CDS	65.0%
	GCCGGTGGAAGGAAAGGAGG+AGG	-	chr4.1:36412807-36412826	MS.gene41161:CDS	65.0%
	GGAGCCGGTGGAAGGAAAGG+AGG	-	chr4.1:36412804-36412823	MS.gene41161:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	36412774	36413226	36412774	ID=MS.gene41161
chr4.1	mRNA	36412774	36413226	36412774	ID=MS.gene41161.t1;Parent=MS.gene41161
chr4.1	exon	36412774	36413226	36412774	ID=MS.gene41161.t1.exon1;Parent=MS.gene41161.t1
chr4.1	CDS	36412774	36413226	36412774	ID=cds.MS.gene41161.t1;Parent=MS.gene41161.t1

Gene Sequence

>MS.gene41161

ATGGACGCAAGTACCAAGACCGCGAAGAAAGGAGCCGGTGGAAGGAAAGGAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCTGGTCTTCAATTTCCAGTTGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGAACCGGTGCTCCAGTATACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAGTTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATTATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGAAAATTACTTGCTGGTGTCACAATTGCTCATGGTGGTGTTCTTCCAAACATTAACCCTATCCTTTTGCCTAAGAAGACTGAAAAGGCTGCTCCCGCCAACAAAGAGCCAAAGAAGGCTGGAAAATCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene41161.t1

MDASTKTAKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTEKAAPANKEPKKAGKSPKKA