AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41224


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41224.t1 MTR_1g049100 89.899 198 17 2 1 196 1 197 2.76e-109 311
MS.gene41224.t1 MTR_5g036480 67.511 237 36 4 1 196 1 237 6.06e-89 261
MS.gene41224.t1 MTR_4g106590 59.669 181 66 3 3 179 21 198 9.40e-63 194
MS.gene41224.t1 MTR_3g078613 61.006 159 56 2 7 162 22 177 1.31e-60 187
MS.gene41224.t1 MTR_3g078613 60.625 160 56 3 7 162 22 178 7.98e-59 183
MS.gene41224.t1 MTR_7g490310 51.813 193 78 3 7 184 8 200 2.20e-56 178
MS.gene41224.t1 MTR_8g038620 59.155 142 53 2 7 143 8 149 7.97e-51 162
MS.gene41224.t1 MTR_3g093860 58.696 138 50 3 4 138 14 147 1.01e-49 158
MS.gene41224.t1 MTR_3g015490 52.903 155 61 3 7 152 3 154 8.39e-46 149
MS.gene41224.t1 MTR_3g088630 50.676 148 57 3 6 138 20 166 8.62e-43 143
MS.gene41224.t1 MTR_6g007460 41.622 185 52 4 7 138 10 191 2.63e-35 123
MS.gene41224.t1 MTR_4g061360 35.075 134 75 2 8 141 89 210 1.82e-17 80.1
MS.gene41224.t1 MTR_3g092780 29.932 147 88 2 8 151 56 190 5.36e-17 78.6
MS.gene41224.t1 MTR_3g092780 29.932 147 88 2 8 151 56 190 6.16e-17 78.6
MS.gene41224.t1 MTR_3g092780 29.730 148 88 2 8 151 56 191 9.80e-17 77.8
MS.gene41224.t1 MTR_3g092780 29.730 148 88 2 8 151 56 191 1.09e-16 77.8
MS.gene41224.t1 MTR_7g118260 31.757 148 87 3 14 161 22 155 6.08e-16 75.5
MS.gene41224.t1 MTR_4g051330 52.239 67 29 1 89 152 77 143 5.83e-15 69.3
MS.gene41224.t1 MTR_7g118260 32.308 130 74 3 14 143 22 137 2.14e-14 71.2
MS.gene41224.t1 MTR_8g024260 30.882 136 82 1 8 143 36 159 5.09e-14 70.1
MS.gene41224.t1 MTR_1g067110 28.788 132 82 1 10 141 87 206 2.23e-13 68.2
MS.gene41224.t1 MTR_1g067110 28.788 132 82 1 10 141 87 206 2.66e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41224.t1 AT1G59940 59.406 202 61 5 4 188 29 226 1.82e-71 216
MS.gene41224.t1 AT1G59940 59.406 202 61 5 4 188 121 318 5.68e-70 216
MS.gene41224.t1 AT1G10470 70.199 151 40 3 6 155 18 164 1.75e-69 212
MS.gene41224.t1 AT1G10470 70.199 151 40 3 6 155 32 178 3.66e-69 211
MS.gene41224.t1 AT3G48100 65.563 151 50 1 8 158 25 173 1.16e-63 195
MS.gene41224.t1 AT5G62920 72.656 128 32 1 8 135 25 149 3.45e-61 189
MS.gene41224.t1 AT1G74890 71.318 129 34 1 7 135 17 142 3.43e-59 184
MS.gene41224.t1 AT1G19050 65.441 136 44 1 7 142 23 155 2.41e-58 182
MS.gene41224.t1 AT2G40670 59.259 135 55 0 4 138 25 159 1.32e-54 171
MS.gene41224.t1 AT3G57040 58.993 139 50 2 7 138 8 146 1.87e-53 171
MS.gene41224.t1 AT3G57040 58.993 139 50 2 7 138 8 146 1.87e-53 171
MS.gene41224.t1 AT3G56380 61.194 134 48 2 6 138 18 148 2.28e-53 167
MS.gene41224.t1 AT2G41310 59.441 143 52 2 1 138 3 144 4.94e-53 169
MS.gene41224.t1 AT2G40670 58.824 136 55 1 4 138 25 160 8.22e-53 167
MS.gene41224.t1 AT3G56380 60.741 135 48 3 6 138 18 149 1.55e-51 163
MS.gene41224.t1 AT1G10470 56.044 91 30 6 70 155 17 102 1.68e-23 92.4
MS.gene41224.t1 AT5G24470 30.216 139 83 3 8 146 52 176 2.20e-15 73.9
MS.gene41224.t1 AT2G46790 31.343 134 80 2 8 141 37 158 2.31e-15 73.9
MS.gene41224.t1 AT2G46790 31.343 134 80 2 8 141 37 158 2.79e-15 73.6
MS.gene41224.t1 AT5G60100 34.848 132 74 2 10 141 66 185 1.88e-14 71.2
MS.gene41224.t1 AT5G60100 34.848 132 74 2 10 141 66 185 1.88e-14 71.2
MS.gene41224.t1 AT5G60100 34.848 132 74 2 10 141 66 185 1.88e-14 71.2
MS.gene41224.t1 AT5G60100 34.848 132 74 2 10 141 66 185 3.07e-14 70.9
MS.gene41224.t1 AT5G60100 34.848 132 74 2 10 141 66 185 3.16e-14 70.5
MS.gene41224.t1 AT5G60100 34.848 132 74 2 10 141 66 185 3.16e-14 70.5
MS.gene41224.t1 AT5G60100 34.848 132 74 2 10 141 66 185 3.16e-14 70.5
MS.gene41224.t1 AT2G07440 60.000 50 20 0 89 138 9 58 1.98e-13 64.7
MS.gene41224.t1 AT5G02810 28.358 134 84 1 8 141 78 199 1.03e-12 66.2

Find 39 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGTGATTACATGCTTCTTC+TGG 0.290924 1.4:-35987961 None:intergenic
ATTGAAAATATTCAGATGTT+TGG 0.307010 1.4:+35987825 MS.gene41224:intron
AACTCATAACCAGTCATTTC+TGG 0.328147 1.4:-35986970 None:intergenic
TTTGTTGCCTTAGCCTTTGA+AGG 0.366124 1.4:+35986918 MS.gene41224:intron
CTCAAAATTTCTGCTTGTAA+AGG 0.386939 1.4:+35986513 MS.gene41224:CDS
TTCTGAAGAGGTTCATGTAT+TGG 0.402386 1.4:+35986446 MS.gene41224:CDS
TACTGGAATTTCCTTAAAAG+TGG 0.438337 1.4:-35987127 None:intergenic
TTAGTTACTGCTGTGGATAG+TGG 0.438893 1.4:+35986739 MS.gene41224:intron
TTCACATCACATAACTTCAC+TGG 0.475573 1.4:-35987877 None:intergenic
ATAACAGTACCTGTCTATGC+GGG 0.487586 1.4:-35987178 None:intergenic
TCAGAAGACATAATCACTAC+TGG 0.489512 1.4:-35987144 None:intergenic
ATGTGAAGGTGATGATGAAA+TGG 0.496627 1.4:-35987998 None:intergenic
ATATTCTGCCCCGCATAGAC+AGG 0.510615 1.4:+35987169 MS.gene41224:CDS
ATATTCAGATGTTTGGAAGA+AGG 0.511460 1.4:+35987832 MS.gene41224:intron
TATTGCATGCCAGAAATGAC+TGG 0.520879 1.4:+35986961 MS.gene41224:CDS
TGCTTGATTCTTCAATCAGA+CGG 0.521707 1.4:+35988049 MS.gene41224:CDS
TTGATCGGAAAGTTATTGAA+AGG 0.524082 1.4:+35986487 MS.gene41224:CDS
TGACAACCCAAATAGTCATT+CGG 0.532453 1.4:-35988088 None:intergenic
ATAACAAGATCCACCTTCAA+AGG 0.539532 1.4:-35986931 None:intergenic
GTTGCCTTAGCCTTTGAAGG+TGG 0.539999 1.4:+35986921 MS.gene41224:intron
TACATGACAACAAGGGAGGT+TGG 0.562595 1.4:+35987913 MS.gene41224:CDS
AGCACACTGGATGCAGACAA+TGG 0.577593 1.4:-35988030 None:intergenic
ACAACAAGGGAGGTTGGAGT+AGG 0.598300 1.4:+35987919 MS.gene41224:CDS
ATGTTTGGAAGAAGGTGCAA+AGG 0.598645 1.4:+35987840 MS.gene41224:CDS
AGAGAGAAGTCTGAATCTGA+TGG 0.604808 1.4:+35986799 MS.gene41224:CDS
GATGGAGACAAATTCTGAAG+AGG 0.610289 1.4:+35986434 None:intergenic
TTGATGATAGTCTCATTGAT+CGG 0.612021 1.4:+35986472 MS.gene41224:CDS
GTGAACTTTAGTTACTGCTG+TGG 0.629072 1.4:+35986732 MS.gene41224:intron
TGAAGGGTTACATGACAACA+AGG 0.633631 1.4:+35987905 MS.gene41224:CDS
GATTGAAGAATCAAGCACAC+TGG 0.636985 1.4:-35988043 None:intergenic
ATGTGATGTGAAACGACTGA+AGG 0.663812 1.4:+35987888 MS.gene41224:CDS
TGTGATGTGAAACGACTGAA+GGG 0.670059 1.4:+35987889 MS.gene41224:CDS
TATAACAGTACCTGTCTATG+CGG 0.670864 1.4:-35987179 None:intergenic
AATGGAGATGATGAATGTGA+AGG 0.695750 1.4:-35988012 None:intergenic
TAACAGTACCTGTCTATGCG+GGG 0.697429 1.4:-35987177 None:intergenic
GGTGATGATGAAATGGACGG+TGG 0.704627 1.4:-35987991 None:intergenic
GGGTTACATGACAACAAGGG+AGG 0.715675 1.4:+35987909 MS.gene41224:CDS
GAAGGTGATGATGAAATGGA+CGG 0.719690 1.4:-35987994 None:intergenic
GAAGGGTTACATGACAACAA+GGG 0.758159 1.4:+35987906 MS.gene41224:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAATTTTAAAAAAATAGT+TGG - chr1.4:35987745-35987764 None:intergenic 10.0%
!! TTTAAGGGATTATTATTATT+AGG + chr1.4:35987217-35987236 MS.gene41224:intron 15.0%
!!! TATAGTAATGATTTTTTCAA+TGG - chr1.4:35987034-35987053 None:intergenic 15.0%
!! ATTGAAAATATTCAGATGTT+TGG + chr1.4:35987825-35987844 MS.gene41224:intron 20.0%
!!! CCAATGATTATACTTTTTAA+TGG + chr1.4:35987410-35987429 MS.gene41224:intron 20.0%
!!! CCATTAAAAAGTATAATCAT+TGG - chr1.4:35987413-35987432 None:intergenic 20.0%
!!! TATTTTTAGACCAAAGAAAA+AGG + chr1.4:35987532-35987551 MS.gene41224:intron 20.0%
! TGAGTTGCTCAAAAAAATTA+AGG + chr1.4:35986987-35987006 MS.gene41224:CDS 25.0%
!! GTTATTGTTATTAGTTACCA+CGG + chr1.4:35987762-35987781 MS.gene41224:intron 25.0%
!! TTATTGTTATTAGTTACCAC+GGG + chr1.4:35987763-35987782 MS.gene41224:intron 25.0%
!! TTGTGTCATTGAAAACATTA+AGG - chr1.4:35987472-35987491 None:intergenic 25.0%
!!! ACGAAATAGATGTTTTTTGA+GGG + chr1.4:35987345-35987364 MS.gene41224:intron 25.0%
!!! TACGAAATAGATGTTTTTTG+AGG + chr1.4:35987344-35987363 MS.gene41224:intron 25.0%
!!! TTTTAAGTGTCCTTTTTCTT+TGG - chr1.4:35987545-35987564 None:intergenic 25.0%
ATATTCAGATGTTTGGAAGA+AGG + chr1.4:35987832-35987851 MS.gene41224:intron 30.0%
CCTGCTTCAAAATGAAATTT+CGG - chr1.4:35987101-35987120 None:intergenic 30.0%
CTCAAAATTTCTGCTTGTAA+AGG + chr1.4:35986513-35986532 MS.gene41224:CDS 30.0%
TACTGGAATTTCCTTAAAAG+TGG - chr1.4:35987130-35987149 None:intergenic 30.0%
TTGATCGGAAAGTTATTGAA+AGG + chr1.4:35986487-35986506 MS.gene41224:CDS 30.0%
! TTGATGATAGTCTCATTGAT+CGG + chr1.4:35986472-35986491 MS.gene41224:CDS 30.0%
!!! CGAAATAGATGTTTTTTGAG+GGG + chr1.4:35987346-35987365 MS.gene41224:intron 30.0%
AACTCATAACCAGTCATTTC+TGG - chr1.4:35986973-35986992 None:intergenic 35.0%
AATGGAGATGATGAATGTGA+AGG - chr1.4:35988015-35988034 None:intergenic 35.0%
ATAACAAGATCCACCTTCAA+AGG - chr1.4:35986934-35986953 None:intergenic 35.0%
GTAAAGGTGAGTTTGTGATT+TGG + chr1.4:35986529-35986548 MS.gene41224:intron 35.0%
TATAACAGTACCTGTCTATG+CGG - chr1.4:35987182-35987201 None:intergenic 35.0%
TCAGAAGACATAATCACTAC+TGG - chr1.4:35987147-35987166 None:intergenic 35.0%
TGCTTGATTCTTCAATCAGA+CGG + chr1.4:35988049-35988068 MS.gene41224:CDS 35.0%
TTCACATCACATAACTTCAC+TGG - chr1.4:35987880-35987899 None:intergenic 35.0%
! ATGTGAAGGTGATGATGAAA+TGG - chr1.4:35988001-35988020 None:intergenic 35.0%
! TCTGATGGTTCTATTGTATG+TGG + chr1.4:35986814-35986833 MS.gene41224:intron 35.0%
! TTCTGAAGAGGTTCATGTAT+TGG + chr1.4:35986446-35986465 MS.gene41224:CDS 35.0%
!!! AATCAGAGCACTGCAATTTT+TGG + chr1.4:35986762-35986781 MS.gene41224:CDS 35.0%
!!! ATCAGAGCACTGCAATTTTT+GGG + chr1.4:35986763-35986782 MS.gene41224:CDS 35.0%
!!! CCGAAATTTCATTTTGAAGC+AGG + chr1.4:35987098-35987117 MS.gene41224:intron 35.0%
AACTTCATCACAATTTCCCG+TGG - chr1.4:35987782-35987801 None:intergenic 40.0%
AGAGAGAAGTCTGAATCTGA+TGG + chr1.4:35986799-35986818 MS.gene41224:CDS 40.0%
ATAACAGTACCTGTCTATGC+GGG - chr1.4:35987181-35987200 None:intergenic 40.0%
ATGTGATGTGAAACGACTGA+AGG + chr1.4:35987888-35987907 MS.gene41224:CDS 40.0%
GAAGGGTTACATGACAACAA+GGG + chr1.4:35987906-35987925 MS.gene41224:CDS 40.0%
GAGTGATTACATGCTTCTTC+TGG - chr1.4:35987964-35987983 None:intergenic 40.0%
GATTGAAGAATCAAGCACAC+TGG - chr1.4:35988046-35988065 None:intergenic 40.0%
GTGAACTTTAGTTACTGCTG+TGG + chr1.4:35986732-35986751 MS.gene41224:intron 40.0%
TATTGCATGCCAGAAATGAC+TGG + chr1.4:35986961-35986980 MS.gene41224:CDS 40.0%
TGAAGGGTTACATGACAACA+AGG + chr1.4:35987905-35987924 MS.gene41224:CDS 40.0%
TGTGATGTGAAACGACTGAA+GGG + chr1.4:35987889-35987908 MS.gene41224:CDS 40.0%
TTAGTTACTGCTGTGGATAG+TGG + chr1.4:35986739-35986758 MS.gene41224:intron 40.0%
! ATGTTTGGAAGAAGGTGCAA+AGG + chr1.4:35987840-35987859 MS.gene41224:CDS 40.0%
! GAAGGTGATGATGAAATGGA+CGG - chr1.4:35987997-35988016 None:intergenic 40.0%
! TTTGTTGCCTTAGCCTTTGA+AGG + chr1.4:35986918-35986937 MS.gene41224:intron 40.0%
!!! TCAGAGCACTGCAATTTTTG+GGG + chr1.4:35986764-35986783 MS.gene41224:CDS 40.0%
AGATCCACCTTCAAAGGCTA+AGG - chr1.4:35986928-35986947 None:intergenic 45.0%
TAACAGTACCTGTCTATGCG+GGG - chr1.4:35987180-35987199 None:intergenic 45.0%
TACATGACAACAAGGGAGGT+TGG + chr1.4:35987913-35987932 MS.gene41224:CDS 45.0%
!! GCAGGAATCGTCCACTTTTA+AGG + chr1.4:35987116-35987135 MS.gene41224:intron 45.0%
!!! AGCACTGCAATTTTTGGGGT+TGG + chr1.4:35986768-35986787 MS.gene41224:CDS 45.0%
!! TAATTTATACTTATATATAA+TGG - chr1.4:35987578-35987597 None:intergenic 5.0%
!!! ATTTTTTTTAAACTTTTTTA+AGG + chr1.4:35987201-35987220 MS.gene41224:intron 5.0%
!!! TTTTTTTTAAACTTTTTTAA+GGG + chr1.4:35987202-35987221 MS.gene41224:intron 5.0%
ACAACAAGGGAGGTTGGAGT+AGG + chr1.4:35987919-35987938 MS.gene41224:CDS 50.0%
AGCACACTGGATGCAGACAA+TGG - chr1.4:35988033-35988052 None:intergenic 50.0%
ATATTCTGCCCCGCATAGAC+AGG + chr1.4:35987169-35987188 MS.gene41224:CDS 50.0%
GGGTTACATGACAACAAGGG+AGG + chr1.4:35987909-35987928 MS.gene41224:CDS 50.0%
! GGTGATGATGAAATGGACGG+TGG - chr1.4:35987994-35988013 None:intergenic 50.0%
! GTTGCCTTAGCCTTTGAAGG+TGG + chr1.4:35986921-35986940 MS.gene41224:intron 50.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 35986435 35988095 35986435 ID=MS.gene41224
chr1.4 mRNA 35986435 35988095 35986435 ID=MS.gene41224.t1;Parent=MS.gene41224
chr1.4 exon 35986435 35986534 35986435 ID=MS.gene41224.t1.exon1;Parent=MS.gene41224.t1
chr1.4 CDS 35986435 35986534 35986435 ID=cds.MS.gene41224.t1;Parent=MS.gene41224.t1
chr1.4 exon 35986743 35986828 35986743 ID=MS.gene41224.t1.exon2;Parent=MS.gene41224.t1
chr1.4 CDS 35986743 35986828 35986743 ID=cds.MS.gene41224.t1;Parent=MS.gene41224.t1
chr1.4 exon 35986931 35987008 35986931 ID=MS.gene41224.t1.exon3;Parent=MS.gene41224.t1
chr1.4 CDS 35986931 35987008 35986931 ID=cds.MS.gene41224.t1;Parent=MS.gene41224.t1
chr1.4 exon 35987120 35987190 35987120 ID=MS.gene41224.t1.exon4;Parent=MS.gene41224.t1
chr1.4 CDS 35987120 35987190 35987120 ID=cds.MS.gene41224.t1;Parent=MS.gene41224.t1
chr1.4 exon 35987840 35988095 35987840 ID=MS.gene41224.t1.exon5;Parent=MS.gene41224.t1
chr1.4 CDS 35987840 35988095 35987840 ID=cds.MS.gene41224.t1;Parent=MS.gene41224.t1
Gene Sequence

>MS.gene41224

ATGGAGACAAATTCTGAAGAGGTTCATGTATTGGCTGTTGATGATAGTCTCATTGATCGGAAAGTTATTGAAAGGTTGCTCAAAATTTCTGCTTGTAAAGGTGAGTTTGTGATTTGGTAAAACAAAAATAAAATTAATAAAATGTTTTTTCATTTGTGAAGATTTTTGAAGATATTCTCATTTGTGAAGATTTTTGTTTAACAAATATAAGTTAAATGAAAAAGAAGTAGAATTTTTTTTAGTGTTATTTTTTTTAATTTCTTTTTAAATTTTAATGTGTTGATGATTTTTGTTTTTGTGAACTTTAGTTACTGCTGTGGATAGTGGAATCAGAGCACTGCAATTTTTGGGGTTGGATGAGCAGAGAGAGAAGTCTGAATCTGATGGTTCTATTGTATGTGGCAATTATTTTATTTTATTTATTTTATAGTTTTTATTCTTTTAATATAATGTTAATTAATTTACTGATTTTATTATTTTATTTTTGTTGCCTTAGCCTTTGAAGGTGGATCTTGTTATTACAGATTATTGCATGCCAGAAATGACTGGTTATGAGTTGCTCAAAAAAATTAAGGTTCAAATTCATTATCACAATTCCATTGAAAAAATCATTACTATAATTTATTTTTCACTCTTTTGATTATATTGTTAGTGAAGTTTGATCCGAAATTTCATTTTGAAGCAGGAATCGTCCACTTTTAAGGAAATTCCAGTAGTGATTATGTCTTCTGAAAATATTCTGCCCCGCATAGACAGGTACTGTTATATTTTTTTTAAACTTTTTTAAGGGATTATTATTATTAGGTAATTTTGACTTTTTATTTTATTTTACTAACACATCTAAATTATGAACATTGTATTTTGTGAAATTTTGATTTTGATAAAAATAATTTTGATTGAAATAATAAGTACGAAATAGATGTTTTTTGAGGGGAAGTTTGAAATAGATGTTTGTGAGTTTTAAATTCTAACAAACCAATGATTATACTTTTTAATGGCACTGATAATATAAAAATAACAGATGTAATATGAATCCTTAATGTTTTCAATGACACAAATAATATAATTTTTTTTATAAGCAATAGTTTATTTAAAAGTATTTTTAGACCAAAGAAAAAGGACACTTAAAAAAATATATCACCATTATATATAAGTATAAATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTCTGTTAAATAAATCATTATTGTTATCTTCAAAAAAATAAATCATTATTGTTAATTTGTTGCATACATAACTTTTAACACAGTAAACCAACTATTTTTTTAAAATTGTTATTGTTATTAGTTACCACGGGAAATTGTGATGAAGTTGAAAAAATTAGTGATTTGTAAATATTGAAAATATTCAGATGTTTGGAAGAAGGTGCAAAGGATTTTATAATAAAGCCAGTGAAGTTATGTGATGTGAAACGACTGAAGGGTTACATGACAACAAGGGAGGTTGGAGTAGGAATCAACAATAAACAGAAGCCAGAAGAAGCATGTAATCACTCATCATCACCACCGTCCATTTCATCATCACCTTCACATTCATCATCTCCATTGTCTGCATCCAGTGTGCTTGATTCTTCAATCAGACGGTTTAAAATGATTGACACCGAATGA

Protein sequence

>MS.gene41224.t1

METNSEEVHVLAVDDSLIDRKVIERLLKISACKVTAVDSGIRALQFLGLDEQREKSESDGSIPLKVDLVITDYCMPEMTGYELLKKIKESSTFKEIPVVIMSSENILPRIDRCLEEGAKDFIIKPVKLCDVKRLKGYMTTREVGVGINNKQKPEEACNHSSSPPSISSSPSHSSSPLSASSVLDSSIRRFKMIDTE