AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044333

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044333.t1 MTR_3g082650 96.884 353 10 1 1 353 1 352 0.0 649
MS.gene044333.t1 MTR_6g004810 65.534 206 68 2 11 216 38 240 7.65e-89 272
MS.gene044333.t1 MTR_3g055990 37.500 304 154 10 18 292 6 302 9.90e-50 173
MS.gene044333.t1 MTR_3g055990 37.500 304 154 10 18 292 6 302 1.05e-49 173
MS.gene044333.t1 MTR_2g026180 38.683 243 113 6 17 239 3 229 1.10e-49 173
MS.gene044333.t1 MTR_5g097580 38.776 294 153 10 18 288 6 295 1.32e-49 173
MS.gene044333.t1 MTR_5g097580 38.776 294 153 10 18 288 6 295 1.60e-49 173
MS.gene044333.t1 MTR_1g073690 36.508 189 101 2 18 188 6 193 1.85e-44 159
MS.gene044333.t1 MTR_3g103420 35.106 188 102 2 18 186 10 196 5.59e-38 139
MS.gene044333.t1 MTR_5g017280 32.143 168 112 1 19 186 5 170 4.37e-31 121
MS.gene044333.t1 MTR_5g017280 32.143 168 112 1 19 186 5 170 7.61e-31 121
MS.gene044333.t1 MTR_2g026180 35.057 174 77 6 86 239 2 159 2.17e-21 94.7
MS.gene044333.t1 MTR_2g026180 44.444 99 51 2 4 98 19 117 7.48e-20 90.5
MS.gene044333.t1 MTR_4g088010 31.304 230 129 9 19 226 77 299 4.72e-20 90.5
MS.gene044333.t1 MTR_4g104470 27.684 177 120 3 19 187 123 299 4.06e-18 84.0
MS.gene044333.t1 MTR_4g122650 29.752 242 129 9 9 226 71 295 2.45e-16 80.9
MS.gene044333.t1 MTR_3g027140 25.253 198 127 4 3 182 41 235 6.30e-14 70.9
MS.gene044333.t1 MTR_7g118120 29.050 179 113 6 19 192 105 274 5.11e-13 70.1
MS.gene044333.t1 MTR_7g107580 25.738 237 140 10 19 236 17 236 1.48e-12 68.6
MS.gene044333.t1 MTR_7g107580 25.738 237 140 10 19 236 17 236 1.64e-12 68.6
MS.gene044333.t1 MTR_7g107580 25.316 237 142 9 19 236 17 237 3.50e-12 67.4
MS.gene044333.t1 MTR_4g070190 50.847 59 29 0 19 77 9 67 5.18e-12 63.9
MS.gene044333.t1 MTR_4g070080 49.153 59 30 0 19 77 7 65 5.87e-12 62.8
MS.gene044333.t1 MTR_1g045510 29.114 158 101 3 37 187 132 285 9.98e-12 65.1
MS.gene044333.t1 MTR_8g024520 40.909 66 39 0 19 84 26 91 1.12e-11 65.1
MS.gene044333.t1 MTR_8g024520 40.909 66 39 0 19 84 26 91 1.14e-11 65.1
MS.gene044333.t1 MTR_4g070080 49.153 59 30 0 19 77 7 65 1.34e-11 62.8
MS.gene044333.t1 MTR_7g117495 42.188 64 37 0 19 82 19 82 1.40e-11 64.3
MS.gene044333.t1 MTR_3g075500 23.333 180 127 3 18 186 109 288 1.50e-11 64.7
MS.gene044333.t1 MTR_4g035310 40.909 66 39 0 19 84 17 82 1.80e-11 64.3
MS.gene044333.t1 MTR_7g117495 42.188 64 37 0 19 82 19 82 1.81e-11 63.9
MS.gene044333.t1 MTR_4g035310 40.909 66 39 0 19 84 17 82 2.09e-11 63.9
MS.gene044333.t1 MTR_3g116130 37.879 66 41 0 19 84 30 95 2.36e-11 63.9
MS.gene044333.t1 MTR_4g070140 45.000 60 33 0 19 78 9 68 2.54e-11 62.4
MS.gene044333.t1 MTR_4g052940 26.923 156 96 7 9 161 107 247 2.99e-11 64.7
MS.gene044333.t1 MTR_5g088220 25.000 160 112 2 19 170 166 325 4.69e-11 64.3
MS.gene044333.t1 MTR_1g064230 25.888 197 114 4 19 186 85 278 5.11e-11 63.2
MS.gene044333.t1 MTR_3g116130 37.879 66 41 0 19 84 30 95 6.64e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044333.t1 AT3G13224 59.178 365 121 9 5 353 6 358 1.16e-101 304
MS.gene044333.t1 AT3G13224 58.689 351 117 9 19 353 11 349 3.01e-95 287
MS.gene044333.t1 AT3G13224 72.067 179 48 1 5 181 6 184 1.03e-88 266
MS.gene044333.t1 AT5G40490 67.172 198 60 4 8 202 31 226 1.49e-81 254
MS.gene044333.t1 AT5G40490 65.027 183 60 3 22 202 29 209 1.12e-73 234
MS.gene044333.t1 AT1G17640 51.892 185 86 1 17 198 65 249 3.54e-66 213
MS.gene044333.t1 AT1G17640 51.892 185 86 1 17 198 65 249 3.54e-66 213
MS.gene044333.t1 AT1G17640 51.892 185 86 1 17 198 65 249 3.54e-66 213
MS.gene044333.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.54e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.51e-19 88.2
MS.gene044333.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.54e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.51e-19 88.2
MS.gene044333.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.54e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.51e-19 88.2
MS.gene044333.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.54e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.51e-19 88.2
MS.gene044333.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.54e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.51e-19 88.2
MS.gene044333.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.37e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.93e-19 87.8
MS.gene044333.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.37e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.93e-19 87.8
MS.gene044333.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.37e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.93e-19 87.8
MS.gene044333.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.37e-49 171
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.93e-19 87.8
MS.gene044333.t1 AT4G26650 40.933 193 94 2 18 191 12 203 1.72e-47 167
MS.gene044333.t1 AT4G26650 44.444 99 54 1 11 108 112 210 4.31e-19 88.6
MS.gene044333.t1 AT4G26650 40.933 193 94 2 18 191 15 206 1.92e-47 167
MS.gene044333.t1 AT4G26650 44.444 99 54 1 11 108 115 213 4.17e-19 88.6
MS.gene044333.t1 AT4G14300 40.107 187 95 2 18 188 6 191 6.29e-46 162
MS.gene044333.t1 AT4G14300 40.107 187 95 2 18 188 6 191 6.29e-46 162
MS.gene044333.t1 AT2G33410 40.642 187 94 3 18 188 6 191 6.10e-45 159
MS.gene044333.t1 AT2G33410 46.392 97 51 1 3 98 95 191 1.32e-19 89.7
MS.gene044333.t1 AT3G07810 35.938 256 139 6 18 256 6 253 7.48e-45 160
MS.gene044333.t1 AT3G07810 35.938 256 139 6 18 256 6 253 8.52e-45 160
MS.gene044333.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.35e-42 153
MS.gene044333.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.93e-13 70.9
MS.gene044333.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.35e-42 153
MS.gene044333.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.93e-13 70.9
MS.gene044333.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.35e-42 153
MS.gene044333.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.93e-13 70.9
MS.gene044333.t1 AT5G55550 33.033 333 141 8 18 280 6 326 2.22e-41 151
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 148 245 1.02e-18 87.4
MS.gene044333.t1 AT5G55550 33.033 333 141 8 18 280 6 326 3.45e-41 151
MS.gene044333.t1 AT5G55550 43.878 98 54 1 9 105 148 245 1.17e-18 87.4
MS.gene044333.t1 AT5G47620 34.296 277 132 9 19 251 7 277 1.26e-37 140
MS.gene044333.t1 AT1G58470 31.624 234 119 3 19 226 7 225 5.04e-34 129
MS.gene044333.t1 AT1G58470 35.185 108 68 1 102 209 2 107 4.13e-11 63.9
MS.gene044333.t1 AT1G58470 31.624 234 119 3 19 226 7 225 5.04e-34 129
MS.gene044333.t1 AT1G58470 35.185 108 68 1 102 209 2 107 4.13e-11 63.9
MS.gene044333.t1 AT4G36960 31.148 183 124 1 19 201 4 184 3.39e-31 121
MS.gene044333.t1 AT4G36960 31.148 183 124 1 19 201 4 184 3.39e-31 121
MS.gene044333.t1 AT5G47620 42.623 122 53 4 1 108 27 145 3.18e-20 90.9
MS.gene044333.t1 AT3G15010 30.952 168 111 2 19 182 76 242 5.50e-19 87.8
MS.gene044333.t1 AT3G15010 30.952 168 111 2 19 182 76 242 5.50e-19 87.8
MS.gene044333.t1 AT5G50250 27.011 174 121 2 18 185 113 286 3.27e-18 84.3
MS.gene044333.t1 AT4G00830 32.044 181 105 7 19 192 117 286 2.22e-17 83.6
MS.gene044333.t1 AT4G00830 24.597 248 150 9 18 255 196 416 6.18e-11 63.9
MS.gene044333.t1 AT4G00830 32.044 181 105 7 19 192 117 286 2.22e-17 83.6
MS.gene044333.t1 AT4G00830 24.597 248 150 9 18 255 196 416 6.18e-11 63.9
MS.gene044333.t1 AT4G00830 32.044 181 105 7 19 192 117 286 2.22e-17 83.6
MS.gene044333.t1 AT4G00830 24.597 248 150 9 18 255 196 416 6.18e-11 63.9
MS.gene044333.t1 AT4G24770 27.119 177 121 4 18 186 150 326 3.26e-17 82.0
MS.gene044333.t1 AT4G24770 27.119 177 121 4 18 186 150 326 3.26e-17 82.0
MS.gene044333.t1 AT4G00830 31.111 180 108 6 19 192 117 286 4.86e-17 82.4
MS.gene044333.t1 AT4G00830 24.597 248 150 9 18 255 196 416 5.12e-11 64.3
MS.gene044333.t1 AT3G52150 27.835 194 119 5 7 182 63 253 2.23e-15 75.5
MS.gene044333.t1 AT3G52150 27.835 194 119 5 7 182 63 253 2.23e-15 75.5
MS.gene044333.t1 AT3G26420 38.750 80 49 0 107 186 8 87 3.32e-15 74.7
MS.gene044333.t1 AT2G37220 25.521 192 118 2 19 185 92 283 3.69e-15 75.5
MS.gene044333.t1 AT5G04280 43.038 79 43 1 19 95 8 86 7.18e-15 74.7
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT3G56860 26.685 356 201 12 19 343 141 467 3.16e-14 73.9
MS.gene044333.t1 AT4G00830 29.189 185 97 7 19 192 117 278 5.31e-14 73.2
MS.gene044333.t1 AT4G00830 29.189 185 97 7 19 192 117 278 5.31e-14 73.2
MS.gene044333.t1 AT1G03457 28.959 221 132 9 19 225 13 222 9.23e-14 72.4
MS.gene044333.t1 AT4G16280 27.907 301 166 13 13 295 115 382 1.02e-13 72.8
MS.gene044333.t1 AT4G16280 27.907 301 166 13 13 295 115 382 1.14e-13 72.4
MS.gene044333.t1 AT4G16280 27.815 302 167 13 12 295 39 307 1.38e-13 72.4
MS.gene044333.t1 AT1G60650 41.379 87 49 1 11 95 5 91 2.72e-13 70.1
MS.gene044333.t1 AT1G60650 41.379 87 49 1 11 95 5 91 2.72e-13 70.1
MS.gene044333.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.45e-13 70.1
MS.gene044333.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.45e-13 70.1
MS.gene044333.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.45e-13 70.1
MS.gene044333.t1 AT2G46780 40.909 66 39 0 19 84 23 88 1.33e-12 68.6
MS.gene044333.t1 AT2G46780 40.909 66 39 0 19 84 23 88 1.33e-12 68.6
MS.gene044333.t1 AT1G03457 27.826 230 132 9 19 225 13 231 1.39e-12 68.9
MS.gene044333.t1 AT4G03110 23.661 224 139 8 19 226 19 226 1.62e-12 68.6
MS.gene044333.t1 AT4G03110 23.661 224 139 8 19 226 19 226 3.68e-12 67.4
MS.gene044333.t1 AT4G03110 23.661 224 139 8 19 226 19 226 3.86e-12 67.4
MS.gene044333.t1 AT4G39260 34.177 79 52 0 107 185 7 85 9.21e-12 61.2
MS.gene044333.t1 AT4G39260 36.585 82 48 2 19 100 7 84 8.93e-11 58.5
MS.gene044333.t1 AT1G76460 40.909 66 39 0 19 84 25 90 9.88e-12 65.5
MS.gene044333.t1 AT1G71770 25.389 193 119 7 3 177 222 407 1.32e-11 66.2
MS.gene044333.t1 AT1G71770 25.389 193 119 7 3 177 222 407 1.32e-11 66.2
MS.gene044333.t1 AT1G11650 25.571 219 125 5 20 217 157 358 1.72e-11 65.5
MS.gene044333.t1 AT4G34110 24.365 197 129 5 3 181 200 394 6.04e-11 64.3
MS.gene044333.t1 AT3G23830 34.066 91 60 0 103 193 32 122 7.47e-11 60.1
MS.gene044333.t1 AT3G23830 34.066 91 60 0 103 193 32 122 7.47e-11 60.1
MS.gene044333.t1 AT5G59865 39.506 81 49 0 107 187 27 107 8.52e-11 59.3
MS.gene044333.t1 AT5G61030 30.769 104 70 1 84 185 16 119 9.48e-11 62.8
MS.gene044333.t1 AT5G61030 30.769 104 70 1 84 185 16 119 9.48e-11 62.8

Find 130 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATTTGGTAGTGATTCT+AGG 0.203855 3.4:+77886942 MS.gene044333:CDS
ACCGGTCGTCCTCGAGGATT+TGG 0.205607 3.4:+77885834 MS.gene044333:CDS
CGTTCTTACAATGGCGGTTT+TGG 0.224937 3.4:+77886968 MS.gene044333:CDS
GATACCTAGGGTTGTATCTT+TGG 0.238318 3.4:-77885661 None:intergenic
TCTGGCCCTGGTGCTGGTTT+TGG 0.239203 3.4:+77887328 MS.gene044333:CDS
TGTTGTTGATCAAGTTATTC+AGG 0.257520 3.4:+77885881 MS.gene044333:CDS
TATAGGTCGCTTGGAGGTTT+TGG 0.267096 3.4:+77887046 MS.gene044333:CDS
GGTGGTAGGTTTAGCGATTA+TGG 0.272361 3.4:+77887067 MS.gene044333:CDS
GATGAATTTGAAGATTTCTT+TGG 0.276198 3.4:-77886911 None:intergenic
GGCCCATATGGGAGAGGTGG+TGG 0.277336 3.4:+77887259 MS.gene044333:CDS
GATGATTTCAGTGGTGGTTT+TGG 0.287960 3.4:+77887106 MS.gene044333:CDS
GGATATGGTGGTGGTGGGTT+AGG 0.316266 3.4:+77887238 MS.gene044333:CDS
TCCGGCGACGGCGCCAGTCC+CGG 0.317544 3.4:+77885527 MS.gene044333:CDS
CCCATATGGGAGAGGTGGTG+GGG 0.320586 3.4:+77887261 MS.gene044333:CDS
TATTCTAGTAGGGGAGGTTA+TGG 0.322189 3.4:+77887367 MS.gene044333:CDS
GGTGGATTTGACGATTCTTA+TGG 0.325447 3.4:+77886989 MS.gene044333:CDS
GGTGGTGGGTTAGGCCCATA+TGG 0.328329 3.4:+77887247 MS.gene044333:CDS
ACTAAGCGTTCCCGTGGATT+TGG 0.349095 3.4:+77886462 MS.gene044333:CDS
ATTCTAGTAGGGGAGGTTAT+GGG 0.359170 3.4:+77887368 MS.gene044333:CDS
GGCCTTGGTGGTGGATATGG+TGG 0.362798 3.4:+77887226 MS.gene044333:CDS
GTGAAGTACTTTGAAAGGTA+TGG 0.367146 3.4:+77885774 MS.gene044333:CDS
CCGGTCGTCCTCGAGGATTT+GGG 0.373418 3.4:+77885835 MS.gene044333:CDS
GTTATGGCCCTACATCTTAT+AGG 0.373426 3.4:+77887029 MS.gene044333:CDS
CCGGCGACGGCGCCAGTCCC+GGG 0.373625 3.4:+77885528 MS.gene044333:CDS
ATAACCTCCCCTACTAGAAT+AGG 0.380687 3.4:-77887365 None:intergenic
GTCGCCGGAGTGAGGGTTAT+CGG 0.392798 3.4:-77885513 None:intergenic
TATGGAGGCAGTGGTGGTGC+TGG 0.406140 3.4:+77887133 MS.gene044333:CDS
TTCATTTGGTAGTGATTCTA+GGG 0.412071 3.4:+77886943 MS.gene044333:CDS
CTTGGTGGTGGATATGGTGG+TGG 0.412985 3.4:+77887229 MS.gene044333:CDS
TTAGTTTGAGATGCACCCTT+TGG 0.413443 3.4:-77886066 None:intergenic
TGATGGGAACATGATTGATA+TGG 0.416351 3.4:+77886530 MS.gene044333:CDS
TATGAATCTGGCCCTGGTGC+TGG 0.417018 3.4:+77887322 MS.gene044333:CDS
CTCAAAGCATGGGAATGTTA+TGG 0.425693 3.4:+77886413 MS.gene044333:CDS
GGTTATGGTGGTCCAGGCAC+TGG 0.434215 3.4:+77887292 MS.gene044333:CDS
TGAAGTACTTTGAAAGGTAT+GGG 0.436031 3.4:+77885775 MS.gene044333:CDS
CCCGGGACTGGCGCCGTCGC+CGG 0.436702 3.4:-77885528 None:intergenic
GCCCATATGGGAGAGGTGGT+GGG 0.438600 3.4:+77887260 MS.gene044333:CDS
ACTGGTGGAGGTTATGAATC+TGG 0.439273 3.4:+77887310 MS.gene044333:CDS
CCATATGGGAGAGGTGGTGG+GGG 0.441700 3.4:+77887262 MS.gene044333:CDS
CACGGGAACGCTTAGTGGTA+TGG 0.442562 3.4:-77886455 None:intergenic
TTCGGTTACTCTGGCCGCTA+TGG 0.446589 3.4:+77887190 MS.gene044333:CDS
GAAGAGAGCGAACCCGGGAC+TGG 0.447616 3.4:-77885540 None:intergenic
GGTGGTGCTGGTGGTTATGC+GGG 0.451484 3.4:+77887145 MS.gene044333:CDS
TTTGGAGGTTATGGAGGCAG+TGG 0.453260 3.4:+77887124 MS.gene044333:CDS
GGTGGTGGATATGGTGGTGG+TGG 0.458107 3.4:+77887232 MS.gene044333:CDS
CCCCACCACCTCTCCCATAT+GGG 0.460579 3.4:-77887261 None:intergenic
CAGGAGGACCCTATTCTAGT+AGG 0.461637 3.4:+77887356 MS.gene044333:CDS
CCCAAATCCTCGAGGACGAC+CGG 0.461844 3.4:-77885835 None:intergenic
AAATCCACGGGAACGCTTAG+TGG 0.462420 3.4:-77886460 None:intergenic
GTGGTGGATATGGTGGTGGT+GGG 0.463417 3.4:+77887233 MS.gene044333:CDS
GGTGGTCCAGGCACTGGTGG+AGG 0.471128 3.4:+77887298 MS.gene044333:CDS
TCATAAAACTATCTTGTGTA+AGG 0.471625 3.4:-77887409 None:intergenic
TAACCTCCCCTACTAGAATA+GGG 0.473608 3.4:-77887364 None:intergenic
GGCCGCTATGGATCCTACAT+GGG 0.474978 3.4:+77887202 MS.gene044333:CDS
AAACCTTCAGATACAGTTGC+TGG 0.484805 3.4:-77886126 None:intergenic
TTTAGCGATTATGGTGGGTA+TGG 0.487404 3.4:+77887076 MS.gene044333:CDS
GTTGATAATTTGTTAGCTGA+TGG 0.487526 3.4:+77886513 MS.gene044333:CDS
GGTGGTTATGCGGGCTATAG+AGG 0.488404 3.4:+77887154 MS.gene044333:CDS
CTGGCGCCGTCGCCGGAGTG+AGG 0.489242 3.4:-77885521 None:intergenic
TGGCCGCTATGGATCCTACA+TGG 0.493274 3.4:+77887201 MS.gene044333:CDS
TCATAACCTCCACCAGTGCC+TGG 0.495641 3.4:-77887304 None:intergenic
AAGAACTTCTTCTCAAAGCA+TGG 0.497829 3.4:+77886402 MS.gene044333:CDS
TATGGCGGTTATGGTGGTCC+AGG 0.499140 3.4:+77887286 MS.gene044333:CDS
CCAAGCGACCTATAAGATGT+AGG 0.501589 3.4:-77887037 None:intergenic
TGGCGCCGTCGCCGGAGTGA+GGG 0.503462 3.4:-77885520 None:intergenic
ATCATGAAGGATCGACATAC+CGG 0.510399 3.4:+77885816 MS.gene044333:CDS
TTGATAATTTGTTAGCTGAT+GGG 0.512546 3.4:+77886514 MS.gene044333:CDS
TGGTGGTGCTGGTGGTTATG+CGG 0.514299 3.4:+77887144 MS.gene044333:CDS
GGAGGTTATGGAGGCAGTGG+TGG 0.517150 3.4:+77887127 MS.gene044333:CDS
ATCAAGAGGACTATTCCAAA+GGG 0.519300 3.4:+77886051 MS.gene044333:CDS
GTCCCACCAAAACCAGCACC+AGG 0.522725 3.4:-77887334 None:intergenic
AGGAGGACCCTATTCTAGTA+GGG 0.525330 3.4:+77887357 MS.gene044333:CDS
AAAACTATCTTGTGTAAGGA+TGG 0.527528 3.4:-77887405 None:intergenic
CTAGAATCACTACCAAATGA+AGG 0.529653 3.4:-77886941 None:intergenic
GATTGATATGGATGGTACTC+AGG 0.532207 3.4:+77886542 MS.gene044333:CDS
AACTGATTCCGTTATCATGA+AGG 0.535063 3.4:+77885803 MS.gene044333:CDS
GGTGGAGATGATTTCAGTGG+TGG 0.538564 3.4:+77887100 MS.gene044333:CDS
GCCGCTATGGATCCTACATG+GGG 0.538686 3.4:+77887203 MS.gene044333:CDS
GGAGGCAGTGGTGGTGCTGG+TGG 0.540901 3.4:+77887136 MS.gene044333:CDS
AGAGGAGGAGAATCCTCATT+CGG 0.541881 3.4:+77887172 MS.gene044333:CDS
ACATCTTATAGGTCGCTTGG+AGG 0.543240 3.4:+77887040 MS.gene044333:CDS
ATGTCGATCCTTCATGATAA+CGG 0.543779 3.4:-77885811 None:intergenic
GGAGGTTATGAATCTGGCCC+TGG 0.544539 3.4:+77887316 MS.gene044333:CDS
TTCTAGTAGGGGAGGTTATG+GGG 0.546191 3.4:+77887369 MS.gene044333:CDS
GAATCCTCATTCGGTTACTC+TGG 0.546282 3.4:+77887181 MS.gene044333:CDS
CAAGCGACCTATAAGATGTA+GGG 0.548725 3.4:-77887036 None:intergenic
CCTACATCTTATAGGTCGCT+TGG 0.550258 3.4:+77887037 MS.gene044333:CDS
AGAACTTCTTCTCAAAGCAT+GGG 0.550482 3.4:+77886403 MS.gene044333:CDS
AACTACAAAACCAAATCCAC+GGG 0.550513 3.4:-77886472 None:intergenic
TCACATCATCAATGACAAGC+AGG 0.551218 3.4:+77885908 MS.gene044333:CDS
AGCGATTATGGTGGGTATGG+AGG 0.554528 3.4:+77887079 MS.gene044333:CDS
GTGGTGGGTTAGGCCCATAT+GGG 0.555497 3.4:+77887248 MS.gene044333:CDS
GGTAGGTTTAGCGATTATGG+TGG 0.558160 3.4:+77887070 MS.gene044333:CDS
TCATGCAGGAAAATCTTCAT+CGG 0.559658 3.4:+77885630 MS.gene044333:intron
GATAACCCTCACTCCGGCGA+CGG 0.564558 3.4:+77885515 MS.gene044333:CDS
AACGCTTAGTGGTATGGTCG+CGG 0.565548 3.4:-77886449 None:intergenic
TTAGGCCCATATGGGAGAGG+TGG 0.567019 3.4:+77887256 MS.gene044333:CDS
GGGTTAGGCCCATATGGGAG+AGG 0.567087 3.4:+77887253 MS.gene044333:CDS
ATCGGACTTTCTGAAAGCCA+TGG 0.568151 3.4:-77885495 None:intergenic
TATGGTGGTCCAGGCACTGG+TGG 0.570069 3.4:+77887295 MS.gene044333:CDS
GCGGCCAGAGTAACCGAATG+AGG 0.570661 3.4:-77887185 None:intergenic
GTTGTAGGTTGAGATCAAGA+AGG 0.571670 3.4:+77886883 MS.gene044333:intron
CGCTATGGATCCTACATGGG+GGG 0.574927 3.4:+77887205 MS.gene044333:CDS
GATTATGGTGGGTATGGAGG+TGG 0.580479 3.4:+77887082 MS.gene044333:CDS
ATTCCAGCAACTGTATCTGA+AGG 0.581606 3.4:+77886123 MS.gene044333:CDS
AAACTACAAAACCAAATCCA+CGG 0.581859 3.4:-77886473 None:intergenic
TCTAGTAGGGGAGGTTATGG+GGG 0.582748 3.4:+77887370 MS.gene044333:CDS
CATATCAGGTTGAAATCAAG+AGG 0.589740 3.4:+77886037 MS.gene044333:intron
GTAGGTTTAGCGATTATGGT+GGG 0.596984 3.4:+77887071 MS.gene044333:CDS
TGCAGGAAAATCTTCATCGG+AGG 0.597403 3.4:+77885633 MS.gene044333:intron
ATTTGGGTTTATCACTTATG+CGG 0.598016 3.4:+77885851 MS.gene044333:CDS
TCTAGGGCACGTTCTTACAA+TGG 0.598638 3.4:+77886959 MS.gene044333:CDS
GTAGGGCCATAACCTCCAGC+AGG 0.600122 3.4:-77887019 None:intergenic
AATCAAGAGGACTATTCCAA+AGG 0.601418 3.4:+77886050 MS.gene044333:CDS
ATCAGCTAACAAATTATCAA+CGG 0.611456 3.4:-77886511 None:intergenic
AGGGCACGTTCTTACAATGG+CGG 0.614082 3.4:+77886962 MS.gene044333:CDS
CGACATACCGGTCGTCCTCG+AGG 0.619903 3.4:+77885828 MS.gene044333:CDS
GAAGTACTTTGAAAGGTATG+GGG 0.631008 3.4:+77885776 MS.gene044333:CDS
GGACCCTATTCTAGTAGGGG+AGG 0.635400 3.4:+77887361 MS.gene044333:CDS
TTAGCCAAAGATACAACCCT+AGG 0.645736 3.4:+77885657 MS.gene044333:CDS
CATACCACTAAGCGTTCCCG+TGG 0.650362 3.4:+77886456 MS.gene044333:CDS
GGTTATGCGGGCTATAGAGG+AGG 0.660221 3.4:+77887157 MS.gene044333:CDS
GGGAACATGATTGATATGGA+TGG 0.665118 3.4:+77886534 MS.gene044333:CDS
AAGTCCGATAACCCTCACTC+CGG 0.666720 3.4:+77885509 MS.gene044333:CDS
GGAGGACCCTATTCTAGTAG+GGG 0.666818 3.4:+77887358 MS.gene044333:CDS
CCGCTATGGATCCTACATGG+GGG 0.674316 3.4:+77887204 MS.gene044333:CDS
CACCACCATATCCACCACCA+AGG 0.678240 3.4:-77887228 None:intergenic
GGAGGTGGAGATGATTTCAG+TGG 0.679386 3.4:+77887097 MS.gene044333:CDS
TCCCACCAAAACCAGCACCA+GGG 0.688176 3.4:-77887333 None:intergenic
GTGATAAACCCAAATCCTCG+AGG 0.709143 3.4:-77885843 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAGTATTATTTATTTTAT+AGG + chr3.4:77885721-77885740 MS.gene044333:intron 10.0%
!! AGTAGTAATAATAATAATTG+CGG + chr3.4:77886696-77886715 MS.gene044333:intron 15.0%
!! TTCTATTTATGAATGAATCT+AGG + chr3.4:77886845-77886864 MS.gene044333:intron 20.0%
!!! AAAACTAAGAAGATTTTTGT+TGG + chr3.4:77886096-77886115 MS.gene044333:CDS 20.0%
!!! ATTCAGTTGAATTTTTGATT+TGG + chr3.4:77885968-77885987 MS.gene044333:intron 20.0%
!!! TAGTTTGGTTTGTTTATTTA+GGG + chr3.4:77886566-77886585 MS.gene044333:intron 20.0%
!!! TTAGTTTGGTTTGTTTATTT+AGG + chr3.4:77886565-77886584 MS.gene044333:intron 20.0%
!!! TTCAGTTGAATTTTTGATTT+GGG + chr3.4:77885969-77885988 MS.gene044333:intron 20.0%
! AATAGTGAAAAAGAGAAATC+GGG - chr3.4:77885596-77885615 None:intergenic 25.0%
! ATCAGCTAACAAATTATCAA+CGG - chr3.4:77886514-77886533 None:intergenic 25.0%
! GAATAGTGAAAAAGAGAAAT+CGG - chr3.4:77885597-77885616 None:intergenic 25.0%
! GATGAATTTGAAGATTTCTT+TGG - chr3.4:77886914-77886933 None:intergenic 25.0%
!!! TAAATAGAACCTTTTTTCCT+TGG - chr3.4:77886834-77886853 None:intergenic 25.0%
!!! TTGATAATTTGTTAGCTGAT+GGG + chr3.4:77886514-77886533 MS.gene044333:CDS 25.0%
AAACTACAAAACCAAATCCA+CGG - chr3.4:77886476-77886495 None:intergenic 30.0%
ACAAAGTGAAACCGTAATTA+GGG - chr3.4:77885939-77885958 None:intergenic 30.0%
ATAGTGAAAAAGAGAAATCG+GGG - chr3.4:77885595-77885614 None:intergenic 30.0%
ATGAAAAGATGTTGATACCT+AGG - chr3.4:77885677-77885696 None:intergenic 30.0%
TGAAAAGATGTTGATACCTA+GGG - chr3.4:77885676-77885695 None:intergenic 30.0%
TGTTGTTGATCAAGTTATTC+AGG + chr3.4:77885881-77885900 MS.gene044333:CDS 30.0%
! ACTAAGAAGATTTTTGTTGG+TGG + chr3.4:77886099-77886118 MS.gene044333:CDS 30.0%
! AGGGTGTTTAAAATTGAAAC+TGG + chr3.4:77886585-77886604 MS.gene044333:intron 30.0%
! ATTTGGGTTTATCACTTATG+CGG + chr3.4:77885851-77885870 MS.gene044333:CDS 30.0%
! TCATCTTCTTTTCCTTCATT+TGG + chr3.4:77886929-77886948 MS.gene044333:CDS 30.0%
! TGAAGTACTTTGAAAGGTAT+GGG + chr3.4:77885775-77885794 MS.gene044333:CDS 30.0%
!! CTTTTGTGAAGTACTTTGAA+AGG + chr3.4:77885769-77885788 MS.gene044333:CDS 30.0%
!! GTTGATAATTTGTTAGCTGA+TGG + chr3.4:77886513-77886532 MS.gene044333:CDS 30.0%
!! TTCATTTGGTAGTGATTCTA+GGG + chr3.4:77886943-77886962 MS.gene044333:CDS 30.0%
!! TTGATTTGTCTGTTCATATC+AGG + chr3.4:77886023-77886042 MS.gene044333:intron 30.0%
!!! TGTAGTTTTTGATAGCGATA+AGG + chr3.4:77886488-77886507 MS.gene044333:CDS 30.0%
!!! TTTGTTTTCGAGTGAAAGTA+TGG + chr3.4:77886263-77886282 MS.gene044333:intron 30.0%
!!! TTTTTTCCTTGGTTTTGGTT+GGG - chr3.4:77886823-77886842 None:intergenic 30.0%
AAAGTGAGACATCACATTGA+TGG - chr3.4:77886736-77886755 None:intergenic 35.0%
AACTACAAAACCAAATCCAC+GGG - chr3.4:77886475-77886494 None:intergenic 35.0%
AAGAACTTCTTCTCAAAGCA+TGG + chr3.4:77886402-77886421 MS.gene044333:CDS 35.0%
AATCAAGAGGACTATTCCAA+AGG + chr3.4:77886050-77886069 MS.gene044333:CDS 35.0%
AGAACTTCTTCTCAAAGCAT+GGG + chr3.4:77886403-77886422 MS.gene044333:CDS 35.0%
AGCAGGTAAAACCCTAATTA+CGG + chr3.4:77885925-77885944 MS.gene044333:intron 35.0%
AGCTTATTGTATGCTTGTCT+TGG + chr3.4:77886358-77886377 MS.gene044333:intron 35.0%
ATCAAGAGGACTATTCCAAA+GGG + chr3.4:77886051-77886070 MS.gene044333:CDS 35.0%
ATGTCGATCCTTCATGATAA+CGG - chr3.4:77885814-77885833 None:intergenic 35.0%
CAACCAAAACCAAGGAAAAA+AGG + chr3.4:77886822-77886841 MS.gene044333:intron 35.0%
CACAAAGTGAAACCGTAATT+AGG - chr3.4:77885940-77885959 None:intergenic 35.0%
CTAGAATCACTACCAAATGA+AGG - chr3.4:77886944-77886963 None:intergenic 35.0%
GAACATAGGTTAGTGGAAAT+TGG + chr3.4:77886621-77886640 MS.gene044333:intron 35.0%
GCTTATTGTATGCTTGTCTT+GGG + chr3.4:77886359-77886378 MS.gene044333:intron 35.0%
TCATGCAGGAAAATCTTCAT+CGG + chr3.4:77885630-77885649 MS.gene044333:intron 35.0%
TGATGGGAACATGATTGATA+TGG + chr3.4:77886530-77886549 MS.gene044333:CDS 35.0%
! AACTGATTCCGTTATCATGA+AGG + chr3.4:77885803-77885822 MS.gene044333:CDS 35.0%
! GAAGTACTTTGAAAGGTATG+GGG + chr3.4:77885776-77885795 MS.gene044333:CDS 35.0%
! GTGAAGTACTTTGAAAGGTA+TGG + chr3.4:77885774-77885793 MS.gene044333:CDS 35.0%
! TTGACTCGATTCTTTGTTGT+AGG + chr3.4:77886868-77886887 MS.gene044333:intron 35.0%
!! CATATCAGGTTGAAATCAAG+AGG + chr3.4:77886037-77886056 MS.gene044333:intron 35.0%
!! CTCATTTTTCGTTTTCATGC+AGG + chr3.4:77885616-77885635 MS.gene044333:intron 35.0%
!! CTTCATTTGGTAGTGATTCT+AGG + chr3.4:77886942-77886961 MS.gene044333:CDS 35.0%
!!! CTTTTTTCCTTGGTTTTGGT+TGG - chr3.4:77886824-77886843 None:intergenic 35.0%
!!! GAACCTTTTTTCCTTGGTTT+TGG - chr3.4:77886828-77886847 None:intergenic 35.0%
AAACCTTCAGATACAGTTGC+TGG - chr3.4:77886129-77886148 None:intergenic 40.0%
ATAACCTCCCCTACTAGAAT+AGG - chr3.4:77887368-77887387 None:intergenic 40.0%
ATCATGAAGGATCGACATAC+CGG + chr3.4:77885816-77885835 MS.gene044333:CDS 40.0%
ATTCCAGCAACTGTATCTGA+AGG + chr3.4:77886123-77886142 MS.gene044333:CDS 40.0%
ATTCTAGTAGGGGAGGTTAT+GGG + chr3.4:77887368-77887387 MS.gene044333:CDS 40.0%
CAAGCGACCTATAAGATGTA+GGG - chr3.4:77887039-77887058 None:intergenic 40.0%
CTCAAAGCATGGGAATGTTA+TGG + chr3.4:77886413-77886432 MS.gene044333:CDS 40.0%
GATACCTAGGGTTGTATCTT+TGG - chr3.4:77885664-77885683 None:intergenic 40.0%
GATTGATATGGATGGTACTC+AGG + chr3.4:77886542-77886561 MS.gene044333:CDS 40.0%
GGGAACATGATTGATATGGA+TGG + chr3.4:77886534-77886553 MS.gene044333:CDS 40.0%
GTAGGTTTAGCGATTATGGT+GGG + chr3.4:77887071-77887090 MS.gene044333:CDS 40.0%
GTTATGGCCCTACATCTTAT+AGG + chr3.4:77887029-77887048 MS.gene044333:CDS 40.0%
TAACCTCCCCTACTAGAATA+GGG - chr3.4:77887367-77887386 None:intergenic 40.0%
TACTGCTGAACATAGGTTAG+TGG + chr3.4:77886614-77886633 MS.gene044333:intron 40.0%
TATTCTAGTAGGGGAGGTTA+TGG + chr3.4:77887367-77887386 MS.gene044333:CDS 40.0%
TCACATCATCAATGACAAGC+AGG + chr3.4:77885908-77885927 MS.gene044333:CDS 40.0%
TCACATTGATGGAAAGCAAC+AGG - chr3.4:77886725-77886744 None:intergenic 40.0%
TCAGACACTTGCAATAACAC+TGG - chr3.4:77886760-77886779 None:intergenic 40.0%
TTAGCCAAAGATACAACCCT+AGG + chr3.4:77885657-77885676 MS.gene044333:CDS 40.0%
TTGCAAGTGTCTGACATATG+TGG + chr3.4:77886766-77886785 MS.gene044333:intron 40.0%
TTTGACGTGCAAAGATGTTG+TGG + chr3.4:77886645-77886664 MS.gene044333:intron 40.0%
! GATGATTTCAGTGGTGGTTT+TGG + chr3.4:77887106-77887125 MS.gene044333:CDS 40.0%
! GGTGGATTTGACGATTCTTA+TGG + chr3.4:77886989-77887008 MS.gene044333:CDS 40.0%
! GTTGTAGGTTGAGATCAAGA+AGG + chr3.4:77886883-77886902 MS.gene044333:intron 40.0%
! TCTTATGGAAGTTTTCCTGC+TGG + chr3.4:77887004-77887023 MS.gene044333:CDS 40.0%
! TTAGTTTGAGATGCACCCTT+TGG - chr3.4:77886069-77886088 None:intergenic 40.0%
!! TTTAGCGATTATGGTGGGTA+TGG + chr3.4:77887076-77887095 MS.gene044333:CDS 40.0%
AAGTGTCTGACATATGTGGC+GGG + chr3.4:77886770-77886789 MS.gene044333:intron 45.0%
ACATCTTATAGGTCGCTTGG+AGG + chr3.4:77887040-77887059 MS.gene044333:CDS 45.0%
ACTGGTGGAGGTTATGAATC+TGG + chr3.4:77887310-77887329 MS.gene044333:CDS 45.0%
AGAGGAGGAGAATCCTCATT+CGG + chr3.4:77887172-77887191 MS.gene044333:CDS 45.0%
AGGAGGACCCTATTCTAGTA+GGG + chr3.4:77887357-77887376 MS.gene044333:CDS 45.0%
CAAGTGTCTGACATATGTGG+CGG + chr3.4:77886769-77886788 MS.gene044333:intron 45.0%
CCAAGCGACCTATAAGATGT+AGG - chr3.4:77887040-77887059 None:intergenic 45.0%
CCTACATCTTATAGGTCGCT+TGG + chr3.4:77887037-77887056 MS.gene044333:CDS 45.0%
GAATCCTCATTCGGTTACTC+TGG + chr3.4:77887181-77887200 MS.gene044333:CDS 45.0%
GGTAGGTTTAGCGATTATGG+TGG + chr3.4:77887070-77887089 MS.gene044333:CDS 45.0%
GGTGGTAGGTTTAGCGATTA+TGG + chr3.4:77887067-77887086 MS.gene044333:CDS 45.0%
GTGATAAACCCAAATCCTCG+AGG - chr3.4:77885846-77885865 None:intergenic 45.0%
GTGATGCTACTGCTGAACAT+AGG + chr3.4:77886607-77886626 MS.gene044333:intron 45.0%
TATCTGCCCAACCAAAACCA+AGG + chr3.4:77886814-77886833 MS.gene044333:intron 45.0%
TGCAGGAAAATCTTCATCGG+AGG + chr3.4:77885633-77885652 MS.gene044333:intron 45.0%
TTCTAGTAGGGGAGGTTATG+GGG + chr3.4:77887369-77887388 MS.gene044333:CDS 45.0%
! AGTTTTCCTGCTGGAGGTTA+TGG + chr3.4:77887013-77887032 MS.gene044333:CDS 45.0%
! CGTTCTTACAATGGCGGTTT+TGG + chr3.4:77886968-77886987 MS.gene044333:CDS 45.0%
! GGATGGTACTCAGGTTAGTT+TGG + chr3.4:77886551-77886570 MS.gene044333:intron 45.0%
! TATAGGTCGCTTGGAGGTTT+TGG + chr3.4:77887046-77887065 MS.gene044333:CDS 45.0%
! TATGGAAGTTTTCCTGCTGG+AGG + chr3.4:77887007-77887026 MS.gene044333:CDS 45.0%
! TCTAGGGCACGTTCTTACAA+TGG + chr3.4:77886959-77886978 MS.gene044333:CDS 45.0%
!! TCTTACAATGGCGGTTTTGG+TGG + chr3.4:77886971-77886990 MS.gene044333:CDS 45.0%
!!! AGTGGTGGTTTTGGAGGTTA+TGG + chr3.4:77887115-77887134 MS.gene044333:CDS 45.0%
!!! GATTTCAGTGGTGGTTTTGG+AGG + chr3.4:77887109-77887128 MS.gene044333:CDS 45.0%
AAATCCACGGGAACGCTTAG+TGG - chr3.4:77886463-77886482 None:intergenic 50.0%
AACGCTTAGTGGTATGGTCG+CGG - chr3.4:77886452-77886471 None:intergenic 50.0%
AAGAGAGAAGAGAGCGAACC+CGG - chr3.4:77885549-77885568 None:intergenic 50.0%
AAGTCCGATAACCCTCACTC+CGG + chr3.4:77885509-77885528 MS.gene044333:CDS 50.0%
ACTAAGCGTTCCCGTGGATT+TGG + chr3.4:77886462-77886481 MS.gene044333:CDS 50.0%
AGGGCACGTTCTTACAATGG+CGG + chr3.4:77886962-77886981 MS.gene044333:CDS 50.0%
CAGGAGGACCCTATTCTAGT+AGG + chr3.4:77887356-77887375 MS.gene044333:CDS 50.0%
GGAGGACCCTATTCTAGTAG+GGG + chr3.4:77887358-77887377 MS.gene044333:CDS 50.0%
GGAGGTGGAGATGATTTCAG+TGG + chr3.4:77887097-77887116 MS.gene044333:CDS 50.0%
GGTGGAGATGATTTCAGTGG+TGG + chr3.4:77887100-77887119 MS.gene044333:CDS 50.0%
TCTAGTAGGGGAGGTTATGG+GGG + chr3.4:77887370-77887389 MS.gene044333:CDS 50.0%
TTTGGAGGTTATGGAGGCAG+TGG + chr3.4:77887124-77887143 MS.gene044333:CDS 50.0%
!! AGCGATTATGGTGGGTATGG+AGG + chr3.4:77887079-77887098 MS.gene044333:CDS 50.0%
!! GATTATGGTGGGTATGGAGG+TGG + chr3.4:77887082-77887101 MS.gene044333:CDS 50.0%
!!! GGTGGTTTTGGAGGTTATGG+AGG + chr3.4:77887118-77887137 MS.gene044333:CDS 50.0%
AGAGAGAAGAGAGCGAACCC+GGG - chr3.4:77885548-77885567 None:intergenic 55.0%
CACCACCATATCCACCACCA+AGG - chr3.4:77887231-77887250 None:intergenic 55.0%
CACGGGAACGCTTAGTGGTA+TGG - chr3.4:77886458-77886477 None:intergenic 55.0%
CATACCACTAAGCGTTCCCG+TGG + chr3.4:77886456-77886475 MS.gene044333:CDS 55.0%
CCCCCATGTAGGATCCATAG+CGG - chr3.4:77887207-77887226 None:intergenic 55.0%
CCGCTATGGATCCTACATGG+GGG + chr3.4:77887204-77887223 MS.gene044333:CDS 55.0%
CGCTATGGATCCTACATGGG+GGG + chr3.4:77887205-77887224 MS.gene044333:CDS 55.0%
GCCGCTATGGATCCTACATG+GGG + chr3.4:77887203-77887222 MS.gene044333:CDS 55.0%
GGACCCTATTCTAGTAGGGG+AGG + chr3.4:77887361-77887380 MS.gene044333:CDS 55.0%
GGAGGTTATGAATCTGGCCC+TGG + chr3.4:77887316-77887335 MS.gene044333:CDS 55.0%
GGCCGCTATGGATCCTACAT+GGG + chr3.4:77887202-77887221 MS.gene044333:CDS 55.0%
TATGAATCTGGCCCTGGTGC+TGG + chr3.4:77887322-77887341 MS.gene044333:CDS 55.0%
TATGGCGGTTATGGTGGTCC+AGG + chr3.4:77887286-77887305 MS.gene044333:CDS 55.0%
TCATAACCTCCACCAGTGCC+TGG - chr3.4:77887307-77887326 None:intergenic 55.0%
TCCCACCAAAACCAGCACCA+GGG - chr3.4:77887336-77887355 None:intergenic 55.0%
TGGCCGCTATGGATCCTACA+TGG + chr3.4:77887201-77887220 MS.gene044333:CDS 55.0%
TTAGGCCCATATGGGAGAGG+TGG + chr3.4:77887256-77887275 MS.gene044333:CDS 55.0%
TTCGGTTACTCTGGCCGCTA+TGG + chr3.4:77887190-77887209 MS.gene044333:CDS 55.0%
! GGTGGTTATGCGGGCTATAG+AGG + chr3.4:77887154-77887173 MS.gene044333:CDS 55.0%
! GGTTATGCGGGCTATAGAGG+AGG + chr3.4:77887157-77887176 MS.gene044333:CDS 55.0%
!! AGGTCGCTTGGAGGTTTTGG+TGG + chr3.4:77887049-77887068 MS.gene044333:CDS 55.0%
!! CGCTTGGAGGTTTTGGTGGT+AGG + chr3.4:77887053-77887072 MS.gene044333:CDS 55.0%
!! CTTGGTGGTGGATATGGTGG+TGG + chr3.4:77887229-77887248 MS.gene044333:CDS 55.0%
!! GGATATGGTGGTGGTGGGTT+AGG + chr3.4:77887238-77887257 MS.gene044333:CDS 55.0%
!! GTGGTGGATATGGTGGTGGT+GGG + chr3.4:77887233-77887252 MS.gene044333:CDS 55.0%
!! GTGGTGGGTTAGGCCCATAT+GGG + chr3.4:77887248-77887267 MS.gene044333:CDS 55.0%
!! TGGTGGTGCTGGTGGTTATG+CGG + chr3.4:77887144-77887163 MS.gene044333:CDS 55.0%
ACCGGTCGTCCTCGAGGATT+TGG + chr3.4:77885834-77885853 MS.gene044333:CDS 60.0%
CCCAAATCCTCGAGGACGAC+CGG - chr3.4:77885838-77885857 None:intergenic 60.0%
CCCATATGGGAGAGGTGGTG+GGG + chr3.4:77887261-77887280 MS.gene044333:CDS 60.0%
CCCCACCACCTCTCCCATAT+GGG - chr3.4:77887264-77887283 None:intergenic 60.0%
CCGGTCGTCCTCGAGGATTT+GGG + chr3.4:77885835-77885854 MS.gene044333:CDS 60.0%
GATAACCCTCACTCCGGCGA+CGG + chr3.4:77885515-77885534 MS.gene044333:CDS 60.0%
GCCCATATGGGAGAGGTGGT+GGG + chr3.4:77887260-77887279 MS.gene044333:CDS 60.0%
GCGGCCAGAGTAACCGAATG+AGG - chr3.4:77887188-77887207 None:intergenic 60.0%
GGAGGTTATGGAGGCAGTGG+TGG + chr3.4:77887127-77887146 MS.gene044333:CDS 60.0%
GGGTTAGGCCCATATGGGAG+AGG + chr3.4:77887253-77887272 MS.gene044333:CDS 60.0%
GGTTATGGTGGTCCAGGCAC+TGG + chr3.4:77887292-77887311 MS.gene044333:CDS 60.0%
GTAGGGCCATAACCTCCAGC+AGG - chr3.4:77887022-77887041 None:intergenic 60.0%
GTCCCACCAAAACCAGCACC+AGG - chr3.4:77887337-77887356 None:intergenic 60.0%
GTCGCCGGAGTGAGGGTTAT+CGG - chr3.4:77885516-77885535 None:intergenic 60.0%
! CCATATGGGAGAGGTGGTGG+GGG + chr3.4:77887262-77887281 MS.gene044333:CDS 60.0%
! TATGGAGGCAGTGGTGGTGC+TGG + chr3.4:77887133-77887152 MS.gene044333:CDS 60.0%
!! GGCCTTGGTGGTGGATATGG+TGG + chr3.4:77887226-77887245 MS.gene044333:CDS 60.0%
!! GGTGGTGCTGGTGGTTATGC+GGG + chr3.4:77887145-77887164 MS.gene044333:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr3.4:77887232-77887251 MS.gene044333:CDS 60.0%
!! GGTGGTGGGTTAGGCCCATA+TGG + chr3.4:77887247-77887266 MS.gene044333:CDS 60.0%
!! TATGGTGGTCCAGGCACTGG+TGG + chr3.4:77887295-77887314 MS.gene044333:CDS 60.0%
!!! GCCCTGGTGCTGGTTTTGGT+GGG + chr3.4:77887332-77887351 MS.gene044333:CDS 60.0%
!!! GCTGGTTTTGGTGGGACAGG+AGG + chr3.4:77887340-77887359 MS.gene044333:CDS 60.0%
!!! GGTGCTGGTTTTGGTGGGAC+AGG + chr3.4:77887337-77887356 MS.gene044333:CDS 60.0%
!!! TCTGGCCCTGGTGCTGGTTT+TGG + chr3.4:77887328-77887347 MS.gene044333:CDS 60.0%
ACCACCAAGGCCCCCCATGT+AGG - chr3.4:77887218-77887237 None:intergenic 65.0%
CCCCCACCACCTCTCCCATA+TGG - chr3.4:77887265-77887284 None:intergenic 65.0%
CGACATACCGGTCGTCCTCG+AGG + chr3.4:77885828-77885847 MS.gene044333:CDS 65.0%
GAAGAGAGCGAACCCGGGAC+TGG - chr3.4:77885543-77885562 None:intergenic 65.0%
GGATCCTACATGGGGGGCCT+TGG + chr3.4:77887211-77887230 MS.gene044333:CDS 65.0%
GGCCCATATGGGAGAGGTGG+TGG + chr3.4:77887259-77887278 MS.gene044333:CDS 65.0%
GGGGGCTATGGCGGTTATGG+TGG + chr3.4:77887280-77887299 MS.gene044333:CDS 65.0%
GGTGGGGGCTATGGCGGTTA+TGG + chr3.4:77887277-77887296 MS.gene044333:CDS 65.0%
TACATGGGGGGCCTTGGTGG+TGG + chr3.4:77887217-77887236 MS.gene044333:CDS 65.0%
TCCTACATGGGGGGCCTTGG+TGG + chr3.4:77887214-77887233 MS.gene044333:CDS 65.0%
!! AGAGGTGGTGGGGGCTATGG+CGG + chr3.4:77887271-77887290 MS.gene044333:CDS 65.0%
!! GGGGGCCTTGGTGGTGGATA+TGG + chr3.4:77887223-77887242 MS.gene044333:CDS 65.0%
!!! GGCCCTGGTGCTGGTTTTGG+TGG + chr3.4:77887331-77887350 MS.gene044333:CDS 65.0%
! GGAGGCAGTGGTGGTGCTGG+TGG + chr3.4:77887136-77887155 MS.gene044333:CDS 70.0%
!! GGGAGAGGTGGTGGGGGCTA+TGG + chr3.4:77887268-77887287 MS.gene044333:CDS 70.0%
!! GGTGGTCCAGGCACTGGTGG+AGG + chr3.4:77887298-77887317 MS.gene044333:CDS 70.0%
TGGCGCCGTCGCCGGAGTGA+GGG - chr3.4:77885523-77885542 None:intergenic 75.0%
! CTGGCGCCGTCGCCGGAGTG+AGG - chr3.4:77885524-77885543 None:intergenic 80.0%
! TCCGGCGACGGCGCCAGTCC+CGG + chr3.4:77885527-77885546 MS.gene044333:CDS 80.0%
! CCGGCGACGGCGCCAGTCCC+GGG + chr3.4:77885528-77885547 MS.gene044333:CDS 85.0%
!! CCCGGGACTGGCGCCGTCGC+CGG - chr3.4:77885531-77885550 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 77885497 77887423 77885497 ID=MS.gene044333
chr3.4 mRNA 77885497 77887423 77885497 ID=MS.gene044333.t1;Parent=MS.gene044333
chr3.4 exon 77885497 77885549 77885497 ID=MS.gene044333.t1.exon1;Parent=MS.gene044333.t1
chr3.4 CDS 77885497 77885549 77885497 ID=cds.MS.gene044333.t1;Parent=MS.gene044333.t1
chr3.4 exon 77885638 77885678 77885638 ID=MS.gene044333.t1.exon2;Parent=MS.gene044333.t1
chr3.4 CDS 77885638 77885678 77885638 ID=cds.MS.gene044333.t1;Parent=MS.gene044333.t1
chr3.4 exon 77885766 77885929 77885766 ID=MS.gene044333.t1.exon3;Parent=MS.gene044333.t1
chr3.4 CDS 77885766 77885929 77885766 ID=cds.MS.gene044333.t1;Parent=MS.gene044333.t1
chr3.4 exon 77886045 77886144 77886045 ID=MS.gene044333.t1.exon4;Parent=MS.gene044333.t1
chr3.4 CDS 77886045 77886144 77886045 ID=cds.MS.gene044333.t1;Parent=MS.gene044333.t1
chr3.4 exon 77886394 77886563 77886394 ID=MS.gene044333.t1.exon5;Parent=MS.gene044333.t1
chr3.4 CDS 77886394 77886563 77886394 ID=cds.MS.gene044333.t1;Parent=MS.gene044333.t1
chr3.4 exon 77886890 77887423 77886890 ID=MS.gene044333.t1.exon6;Parent=MS.gene044333.t1
chr3.4 CDS 77886890 77887423 77886890 ID=cds.MS.gene044333.t1;Parent=MS.gene044333.t1
Gene Sequence

>MS.gene044333

ATGGCTTTCAGAAAGTCCGATAACCCTCACTCCGGCGACGGCGCCAGTCCCGGGTTCGCTCTCTTCTCTCTTCTTACACATTCAATTCGCTTCGTCCCCGATTTCTCTTTTTCACTATTCTCATTTTTCGTTTTCATGCAGGAAAATCTTCATCGGAGGTTTAGCCAAAGATACAACCCTAGGTATCAACATCTTTTCATTTTTTTGATATTTTTCTTTTAATTTTCAGTATTATTTATTTTATAGGTTTTGATATTTTGATTCTGCAGAGACTTTTGTGAAGTACTTTGAAAGGTATGGGGAGATAACTGATTCCGTTATCATGAAGGATCGACATACCGGTCGTCCTCGAGGATTTGGGTTTATCACTTATGCGGATGCTTCTGTTGTTGATCAAGTTATTCAGGAGAATCACATCATCAATGACAAGCAGGTAAAACCCTAATTACGGTTTCACTTTGTGTTTTGTAGATTCAGTTGAATTTTTGATTTGGGCTGTTTTCAAATGTTTACTTTGTTTTCATTGTTGATTTGTCTGTTCATATCAGGTTGAAATCAAGAGGACTATTCCAAAGGGTGCATCTCAAACTAATGACTTCAAAACTAAGAAGATTTTTGTTGGTGGCATTCCAGCAACTGTATCTGAAGGTTTGTCTTCAGCACTTATAATTTTTTTGATATAATCAATGTTCGTTGTGTGCTTTGATTTGTTTATATAACAATTAGTGTTTATTGTGTGTTTATACAAGTTTGTTAGTCTGTATTGTTTGTTTTCGAGTGAAAGTATGGTATTGTGTGTTAGTGAGCTTGTTAGTTTGTATTGTTTGTTTTCGAGTGAAAGTATGGTATTGTGTGTTATTGAGCTTATTGTATGCTTGTCTTGGGTGTGTGTTTCAGATGAGCTCAAGAACTTCTTCTCAAAGCATGGGAATGTTATGGAGCACGAGATCATCCGCGACCATACCACTAAGCGTTCCCGTGGATTTGGTTTTGTAGTTTTTGATAGCGATAAGGCCGTTGATAATTTGTTAGCTGATGGGAACATGATTGATATGGATGGTACTCAGGTTAGTTTGGTTTGTTTATTTAGGGTGTTTAAAATTGAAACTGGTGATGCTACTGCTGAACATAGGTTAGTGGAAATTGGTTTTGACGTGCAAAGATGTTGTGGAATAATTGATAATAATAATAATAGTAGTAGTAGTAATAATAATAATTGCGGATTCCTGTTGCTTTCCATCAATGTGATGTCTCACTTTGCCAGTGTTATTGCAAGTGTCTGACATATGTGGCGGGCTAACTGCACATTTTATCATTTATCTGCCCAACCAAAACCAAGGAAAAAAGGTTCTATTTATGAATGAATCTAGGTTGACTCGATTCTTTGTTGTAGGTTGAGATCAAGAAGGCTGAACCAAAGAAATCTTCAAATTCATCTTCTTTTCCTTCATTTGGTAGTGATTCTAGGGCACGTTCTTACAATGGCGGTTTTGGTGGATTTGACGATTCTTATGGAAGTTTTCCTGCTGGAGGTTATGGCCCTACATCTTATAGGTCGCTTGGAGGTTTTGGTGGTAGGTTTAGCGATTATGGTGGGTATGGAGGTGGAGATGATTTCAGTGGTGGTTTTGGAGGTTATGGAGGCAGTGGTGGTGCTGGTGGTTATGCGGGCTATAGAGGAGGAGAATCCTCATTCGGTTACTCTGGCCGCTATGGATCCTACATGGGGGGCCTTGGTGGTGGATATGGTGGTGGTGGGTTAGGCCCATATGGGAGAGGTGGTGGGGGCTATGGCGGTTATGGTGGTCCAGGCACTGGTGGAGGTTATGAATCTGGCCCTGGTGCTGGTTTTGGTGGGACAGGAGGACCCTATTCTAGTAGGGGAGGTTATGGGGGCAGTAGTCGTTACCATCCTTACACAAGATAG
Protein sequence

>MS.gene044333.t1

MAFRKSDNPHSGDGASPGKIFIGGLAKDTTLETFVKYFERYGEITDSVIMKDRHTGRPRGFGFITYADASVVDQVIQENHIINDKQVEIKRTIPKGASQTNDFKTKKIFVGGIPATVSEDELKNFFSKHGNVMEHEIIRDHTTKRSRGFGFVVFDSDKAVDNLLADGNMIDMDGTQVEIKKAEPKKSSNSSSFPSFGSDSRARSYNGGFGGFDDSYGSFPAGGYGPTSYRSLGGFGGRFSDYGGYGGGDDFSGGFGGYGGSGGAGGYAGYRGGESSFGYSGRYGSYMGGLGGGYGGGGLGPYGRGGGGYGGYGGPGTGGGYESGPGAGFGGTGGPYSSRGGYGGSSRYHPYTR