AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25697

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25697.t1 MTR_3g082650 97.167 353 9 1 1 353 1 352 0.0 652
MS.gene25697.t1 MTR_6g004810 65.534 206 68 2 11 216 38 240 7.10e-89 272
MS.gene25697.t1 MTR_3g055990 37.500 304 154 10 18 292 6 302 7.58e-50 174
MS.gene25697.t1 MTR_3g055990 37.500 304 154 10 18 292 6 302 7.63e-50 174
MS.gene25697.t1 MTR_2g026180 38.683 243 113 6 17 239 3 229 1.04e-49 173
MS.gene25697.t1 MTR_5g097580 38.776 294 153 10 18 288 6 295 1.04e-49 173
MS.gene25697.t1 MTR_5g097580 38.776 294 153 10 18 288 6 295 1.56e-49 173
MS.gene25697.t1 MTR_1g073690 36.508 189 101 2 18 188 6 193 1.63e-44 159
MS.gene25697.t1 MTR_3g103420 35.106 188 102 2 18 186 10 196 5.36e-38 139
MS.gene25697.t1 MTR_5g017280 32.143 168 112 1 19 186 5 170 4.94e-31 121
MS.gene25697.t1 MTR_5g017280 32.143 168 112 1 19 186 5 170 7.09e-31 121
MS.gene25697.t1 MTR_2g026180 35.057 174 77 6 86 239 2 159 1.78e-21 95.1
MS.gene25697.t1 MTR_2g026180 44.000 100 52 2 3 98 18 117 4.97e-20 90.9
MS.gene25697.t1 MTR_4g088010 31.304 230 129 9 19 226 77 299 5.20e-20 90.1
MS.gene25697.t1 MTR_4g104470 27.684 177 120 3 19 187 123 299 4.26e-18 84.0
MS.gene25697.t1 MTR_4g122650 29.752 242 129 9 9 226 71 295 2.58e-16 80.5
MS.gene25697.t1 MTR_3g027140 25.000 184 121 3 13 182 55 235 1.25e-13 70.1
MS.gene25697.t1 MTR_7g118120 29.050 179 113 6 19 192 105 274 5.45e-13 70.1
MS.gene25697.t1 MTR_7g107580 25.738 237 140 10 19 236 17 236 1.64e-12 68.6
MS.gene25697.t1 MTR_7g107580 25.738 237 140 10 19 236 17 236 1.79e-12 68.2
MS.gene25697.t1 MTR_7g107580 25.316 237 142 9 19 236 17 237 3.41e-12 67.8
MS.gene25697.t1 MTR_4g070190 50.847 59 29 0 19 77 9 67 5.23e-12 63.9
MS.gene25697.t1 MTR_4g070080 49.153 59 30 0 19 77 7 65 5.54e-12 62.8
MS.gene25697.t1 MTR_1g045510 29.114 158 101 3 37 187 132 285 1.01e-11 65.1
MS.gene25697.t1 MTR_8g024520 40.909 66 39 0 19 84 26 91 1.01e-11 65.1
MS.gene25697.t1 MTR_8g024520 40.909 66 39 0 19 84 26 91 1.03e-11 65.1
MS.gene25697.t1 MTR_4g070080 49.153 59 30 0 19 77 7 65 1.37e-11 62.8
MS.gene25697.t1 MTR_3g075500 23.333 180 127 3 18 186 109 288 1.44e-11 64.7
MS.gene25697.t1 MTR_7g117495 42.188 64 37 0 19 82 19 82 1.46e-11 64.3
MS.gene25697.t1 MTR_7g117495 42.188 64 37 0 19 82 19 82 1.83e-11 63.9
MS.gene25697.t1 MTR_4g035310 40.909 66 39 0 19 84 17 82 1.85e-11 64.3
MS.gene25697.t1 MTR_4g035310 40.909 66 39 0 19 84 17 82 1.96e-11 64.3
MS.gene25697.t1 MTR_3g116130 37.879 66 41 0 19 84 30 95 2.27e-11 63.9
MS.gene25697.t1 MTR_4g070140 45.000 60 33 0 19 78 9 68 2.54e-11 62.4
MS.gene25697.t1 MTR_4g052940 26.923 156 96 7 9 161 107 247 2.66e-11 65.1
MS.gene25697.t1 MTR_5g088220 25.000 160 112 2 19 170 166 325 3.81e-11 64.7
MS.gene25697.t1 MTR_1g064230 25.888 197 114 4 19 186 85 278 5.21e-11 63.2
MS.gene25697.t1 MTR_3g116130 37.879 66 41 0 19 84 30 95 6.70e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25697.t1 AT3G13224 59.189 370 122 10 1 353 1 358 3.40e-102 305
MS.gene25697.t1 AT3G13224 58.689 351 117 9 19 353 11 349 3.25e-95 287
MS.gene25697.t1 AT3G13224 71.739 184 49 2 1 181 1 184 4.88e-89 267
MS.gene25697.t1 AT5G40490 67.172 198 60 4 8 202 31 226 1.49e-81 254
MS.gene25697.t1 AT5G40490 65.027 183 60 3 22 202 29 209 8.06e-74 234
MS.gene25697.t1 AT1G17640 51.892 185 86 1 17 198 65 249 2.74e-66 214
MS.gene25697.t1 AT1G17640 51.892 185 86 1 17 198 65 249 2.74e-66 214
MS.gene25697.t1 AT1G17640 51.892 185 86 1 17 198 65 249 2.74e-66 214
MS.gene25697.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene25697.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene25697.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene25697.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene25697.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene25697.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene25697.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene25697.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene25697.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene25697.t1 AT4G26650 40.933 193 94 2 18 191 12 203 1.26e-47 167
MS.gene25697.t1 AT4G26650 44.444 99 54 1 11 108 112 210 3.89e-19 88.6
MS.gene25697.t1 AT4G26650 40.933 193 94 2 18 191 15 206 1.56e-47 167
MS.gene25697.t1 AT4G26650 44.444 99 54 1 11 108 115 213 3.87e-19 88.6
MS.gene25697.t1 AT4G14300 40.107 187 95 2 18 188 6 191 5.05e-46 162
MS.gene25697.t1 AT4G14300 40.107 187 95 2 18 188 6 191 5.05e-46 162
MS.gene25697.t1 AT2G33410 40.642 187 94 3 18 188 6 191 5.05e-45 159
MS.gene25697.t1 AT2G33410 46.939 98 48 2 2 98 97 191 4.42e-19 88.2
MS.gene25697.t1 AT3G07810 35.938 256 139 6 18 256 6 253 5.94e-45 161
MS.gene25697.t1 AT3G07810 35.938 256 139 6 18 256 6 253 6.48e-45 160
MS.gene25697.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.18e-42 153
MS.gene25697.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.85e-13 70.9
MS.gene25697.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.18e-42 153
MS.gene25697.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.85e-13 70.9
MS.gene25697.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.18e-42 153
MS.gene25697.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.85e-13 70.9
MS.gene25697.t1 AT5G55550 33.033 333 141 8 18 280 6 326 1.74e-41 151
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 148 245 9.43e-19 87.8
MS.gene25697.t1 AT5G55550 33.033 333 141 8 18 280 6 326 2.82e-41 151
MS.gene25697.t1 AT5G55550 43.878 98 54 1 9 105 148 245 1.13e-18 87.4
MS.gene25697.t1 AT5G47620 34.296 277 132 9 19 251 7 277 9.60e-38 140
MS.gene25697.t1 AT1G58470 31.624 234 119 3 19 226 7 225 5.15e-34 129
MS.gene25697.t1 AT1G58470 35.185 108 68 1 102 209 2 107 4.17e-11 63.9
MS.gene25697.t1 AT1G58470 31.624 234 119 3 19 226 7 225 5.15e-34 129
MS.gene25697.t1 AT1G58470 35.185 108 68 1 102 209 2 107 4.17e-11 63.9
MS.gene25697.t1 AT4G36960 31.148 183 124 1 19 201 4 184 3.36e-31 121
MS.gene25697.t1 AT4G36960 31.148 183 124 1 19 201 4 184 3.36e-31 121
MS.gene25697.t1 AT5G47620 45.455 99 45 2 19 108 47 145 8.92e-20 89.7
MS.gene25697.t1 AT3G15010 30.952 168 111 2 19 182 76 242 4.55e-19 88.2
MS.gene25697.t1 AT3G15010 30.952 168 111 2 19 182 76 242 4.55e-19 88.2
MS.gene25697.t1 AT5G50250 27.011 174 121 2 18 185 113 286 3.50e-18 84.0
MS.gene25697.t1 AT4G24770 26.425 193 131 5 2 186 137 326 1.87e-17 82.4
MS.gene25697.t1 AT4G24770 26.425 193 131 5 2 186 137 326 1.87e-17 82.4
MS.gene25697.t1 AT4G00830 32.044 181 105 7 19 192 117 286 1.88e-17 84.0
MS.gene25697.t1 AT4G00830 24.597 248 150 9 18 255 196 416 5.50e-11 63.9
MS.gene25697.t1 AT4G00830 32.044 181 105 7 19 192 117 286 1.88e-17 84.0
MS.gene25697.t1 AT4G00830 24.597 248 150 9 18 255 196 416 5.50e-11 63.9
MS.gene25697.t1 AT4G00830 32.044 181 105 7 19 192 117 286 1.88e-17 84.0
MS.gene25697.t1 AT4G00830 24.597 248 150 9 18 255 196 416 5.50e-11 63.9
MS.gene25697.t1 AT4G00830 31.111 180 108 6 19 192 117 286 4.27e-17 82.8
MS.gene25697.t1 AT4G00830 24.597 248 150 9 18 255 196 416 4.31e-11 64.3
MS.gene25697.t1 AT3G52150 27.136 199 124 5 2 182 58 253 2.74e-15 75.1
MS.gene25697.t1 AT3G52150 27.136 199 124 5 2 182 58 253 2.74e-15 75.1
MS.gene25697.t1 AT3G26420 38.750 80 49 0 107 186 8 87 3.07e-15 74.7
MS.gene25697.t1 AT2G37220 25.521 192 118 2 19 185 92 283 4.30e-15 75.1
MS.gene25697.t1 AT5G04280 43.038 79 43 1 19 95 8 86 7.12e-15 74.7
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene25697.t1 AT4G00830 29.189 185 97 7 19 192 117 278 4.51e-14 73.6
MS.gene25697.t1 AT4G00830 29.189 185 97 7 19 192 117 278 4.51e-14 73.6
MS.gene25697.t1 AT1G03457 28.959 221 132 9 19 225 13 222 8.90e-14 72.4
MS.gene25697.t1 AT4G16280 27.907 301 166 13 13 295 115 382 1.10e-13 72.8
MS.gene25697.t1 AT4G16280 27.907 301 166 13 13 295 115 382 1.29e-13 72.4
MS.gene25697.t1 AT4G16280 27.907 301 166 13 13 295 40 307 1.30e-13 72.4
MS.gene25697.t1 AT1G60650 41.379 87 49 1 11 95 5 91 2.57e-13 70.1
MS.gene25697.t1 AT1G60650 41.379 87 49 1 11 95 5 91 2.57e-13 70.1
MS.gene25697.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.51e-13 70.1
MS.gene25697.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.51e-13 70.1
MS.gene25697.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.51e-13 70.1
MS.gene25697.t1 AT2G46780 40.909 66 39 0 19 84 23 88 1.38e-12 68.2
MS.gene25697.t1 AT2G46780 40.909 66 39 0 19 84 23 88 1.38e-12 68.2
MS.gene25697.t1 AT4G03110 23.661 224 139 8 19 226 19 226 1.58e-12 68.6
MS.gene25697.t1 AT1G03457 27.826 230 132 9 19 225 13 231 1.58e-12 68.6
MS.gene25697.t1 AT4G03110 23.661 224 139 8 19 226 19 226 4.21e-12 67.4
MS.gene25697.t1 AT4G03110 23.661 224 139 8 19 226 19 226 4.58e-12 67.4
MS.gene25697.t1 AT4G39260 34.177 79 52 0 107 185 7 85 8.59e-12 61.2
MS.gene25697.t1 AT4G39260 36.585 82 48 2 19 100 7 84 8.84e-11 58.5
MS.gene25697.t1 AT1G76460 40.909 66 39 0 19 84 25 90 9.79e-12 65.5
MS.gene25697.t1 AT1G71770 25.521 192 118 7 4 177 223 407 1.39e-11 66.2
MS.gene25697.t1 AT1G71770 25.521 192 118 7 4 177 223 407 1.39e-11 66.2
MS.gene25697.t1 AT1G11650 25.571 219 125 5 20 217 157 358 1.90e-11 65.1
MS.gene25697.t1 AT3G23830 34.066 91 60 0 103 193 32 122 7.25e-11 60.1
MS.gene25697.t1 AT3G23830 34.066 91 60 0 103 193 32 122 7.25e-11 60.1
MS.gene25697.t1 AT5G59865 39.506 81 49 0 107 187 27 107 7.80e-11 59.3
MS.gene25697.t1 AT5G61030 30.769 104 70 1 84 185 16 119 8.72e-11 62.8
MS.gene25697.t1 AT5G61030 30.769 104 70 1 84 185 16 119 8.72e-11 62.8

Find 130 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGGGTTATCGGACTTTC+TGG 0.192489 3.3:-69404861 None:intergenic
CTTCATTTGGTAGTGATTCT+AGG 0.203855 3.3:+69406302 MS.gene25697:CDS
ACCGGTCGTCCTCGAGGATT+TGG 0.205607 3.3:+69405191 MS.gene25697:CDS
CGTTCTTACAATGGCGGTTT+TGG 0.224937 3.3:+69406328 MS.gene25697:CDS
GATACCTAGGGTTGTATCTT+TGG 0.238318 3.3:-69405018 None:intergenic
TCTGGCCCTGGTGCTGGTTT+TGG 0.239203 3.3:+69406688 MS.gene25697:CDS
TGTTGTTGATCAAGTTATTC+AGG 0.257520 3.3:+69405238 MS.gene25697:CDS
TATAGGTCGCTTGGAGGTTT+TGG 0.267096 3.3:+69406406 MS.gene25697:CDS
GGTGGTAGGTTTAGCGATTA+TGG 0.272361 3.3:+69406427 MS.gene25697:CDS
GATGAATTTGAAGATTTCTT+TGG 0.276198 3.3:-69406271 None:intergenic
GGCCCATATGGGAGAGGTGG+TGG 0.277336 3.3:+69406619 MS.gene25697:CDS
GATGATTTCAGTGGTGGTTT+TGG 0.287960 3.3:+69406466 MS.gene25697:CDS
GGATATGGTGGTGGTGGGTT+AGG 0.316266 3.3:+69406598 MS.gene25697:CDS
TCCGGCGACGGCGCCAGTCC+CGG 0.317544 3.3:+69404884 MS.gene25697:CDS
CCCATATGGGAGAGGTGGTG+GGG 0.320586 3.3:+69406621 MS.gene25697:CDS
TATTCTAGTAGGGGAGGTTA+TGG 0.322189 3.3:+69406727 MS.gene25697:CDS
GGTGGATTTGACGATTCTTA+TGG 0.325447 3.3:+69406349 MS.gene25697:CDS
GGTGGTGGGTTAGGCCCATA+TGG 0.328329 3.3:+69406607 MS.gene25697:CDS
ACTAAGCGTTCCCGTGGATT+TGG 0.349095 3.3:+69405819 MS.gene25697:CDS
ATTCTAGTAGGGGAGGTTAT+GGG 0.359170 3.3:+69406728 MS.gene25697:CDS
GGCCTTGGTGGTGGATATGG+TGG 0.362798 3.3:+69406586 MS.gene25697:CDS
GTGAAGTACTTTGAAAGGTA+TGG 0.367146 3.3:+69405131 MS.gene25697:CDS
CCGGTCGTCCTCGAGGATTT+GGG 0.373418 3.3:+69405192 MS.gene25697:CDS
GTTATGGCCCTACATCTTAT+AGG 0.373426 3.3:+69406389 MS.gene25697:CDS
CCGGCGACGGCGCCAGTCCC+GGG 0.373625 3.3:+69404885 MS.gene25697:CDS
ATAACCTCCCCTACTAGAAT+AGG 0.380687 3.3:-69406725 None:intergenic
GTCGCCGGAGTGAGGGTTAT+CGG 0.392798 3.3:-69404870 None:intergenic
TATGGAGGCAGTGGTGGTGC+TGG 0.406140 3.3:+69406493 MS.gene25697:CDS
TTCATTTGGTAGTGATTCTA+GGG 0.412071 3.3:+69406303 MS.gene25697:CDS
CTTGGTGGTGGATATGGTGG+TGG 0.412985 3.3:+69406589 MS.gene25697:CDS
TTAGTTTGAGATGCACCCTT+TGG 0.413443 3.3:-69405423 None:intergenic
TGATGGGAACATGATTGATA+TGG 0.416351 3.3:+69405887 MS.gene25697:CDS
TATGAATCTGGCCCTGGTGC+TGG 0.417018 3.3:+69406682 MS.gene25697:CDS
CTCAAAGCATGGGAATGTTA+TGG 0.425693 3.3:+69405770 MS.gene25697:CDS
GGTTATGGTGGTCCAGGCAC+TGG 0.434215 3.3:+69406652 MS.gene25697:CDS
TGAAGTACTTTGAAAGGTAT+GGG 0.436031 3.3:+69405132 MS.gene25697:CDS
CCCGGGACTGGCGCCGTCGC+CGG 0.436702 3.3:-69404885 None:intergenic
GCCCATATGGGAGAGGTGGT+GGG 0.438600 3.3:+69406620 MS.gene25697:CDS
ACTGGTGGAGGTTATGAATC+TGG 0.439273 3.3:+69406670 MS.gene25697:CDS
CCATATGGGAGAGGTGGTGG+GGG 0.441700 3.3:+69406622 MS.gene25697:CDS
CACGGGAACGCTTAGTGGTA+TGG 0.442562 3.3:-69405812 None:intergenic
TTCGGTTACTCTGGCCGCTA+TGG 0.446589 3.3:+69406550 MS.gene25697:CDS
GAAGAGAGCGAACCCGGGAC+TGG 0.447616 3.3:-69404897 None:intergenic
GGTGGTGCTGGTGGTTATGC+GGG 0.451484 3.3:+69406505 MS.gene25697:CDS
TTTGGAGGTTATGGAGGCAG+TGG 0.453260 3.3:+69406484 MS.gene25697:CDS
GGTGGTGGATATGGTGGTGG+TGG 0.458107 3.3:+69406592 MS.gene25697:CDS
CCCCACCACCTCTCCCATAT+GGG 0.460579 3.3:-69406621 None:intergenic
CAGGAGGACCCTATTCTAGT+AGG 0.461637 3.3:+69406716 MS.gene25697:CDS
CCCAAATCCTCGAGGACGAC+CGG 0.461844 3.3:-69405192 None:intergenic
AAATCCACGGGAACGCTTAG+TGG 0.462420 3.3:-69405817 None:intergenic
GTGGTGGATATGGTGGTGGT+GGG 0.463417 3.3:+69406593 MS.gene25697:CDS
GGTGGTCCAGGCACTGGTGG+AGG 0.471128 3.3:+69406658 MS.gene25697:CDS
TCATAAAACTATCTTGTGTA+AGG 0.471625 3.3:-69406769 None:intergenic
TAACCTCCCCTACTAGAATA+GGG 0.473608 3.3:-69406724 None:intergenic
GGCCGCTATGGATCCTACAT+GGG 0.474978 3.3:+69406562 MS.gene25697:CDS
AAACCTTCAGATACAGTTGC+TGG 0.484805 3.3:-69405483 None:intergenic
TTTAGCGATTATGGTGGGTA+TGG 0.487404 3.3:+69406436 MS.gene25697:CDS
GTTGATAATTTGTTAGCTGA+TGG 0.487526 3.3:+69405870 MS.gene25697:CDS
GGTGGTTATGCGGGCTATAG+AGG 0.488404 3.3:+69406514 MS.gene25697:CDS
CTGGCGCCGTCGCCGGAGTG+AGG 0.489242 3.3:-69404878 None:intergenic
TGGCCGCTATGGATCCTACA+TGG 0.493274 3.3:+69406561 MS.gene25697:CDS
TCATAACCTCCACCAGTGCC+TGG 0.495641 3.3:-69406664 None:intergenic
AAGAACTTCTTCTCAAAGCA+TGG 0.497829 3.3:+69405759 MS.gene25697:CDS
TATGGCGGTTATGGTGGTCC+AGG 0.499140 3.3:+69406646 MS.gene25697:CDS
CCAAGCGACCTATAAGATGT+AGG 0.501589 3.3:-69406397 None:intergenic
TGGCGCCGTCGCCGGAGTGA+GGG 0.503462 3.3:-69404877 None:intergenic
ATCATGAAGGATCGACATAC+CGG 0.510399 3.3:+69405173 MS.gene25697:CDS
TTGATAATTTGTTAGCTGAT+GGG 0.512546 3.3:+69405871 MS.gene25697:CDS
TGGTGGTGCTGGTGGTTATG+CGG 0.514299 3.3:+69406504 MS.gene25697:CDS
GGAGGTTATGGAGGCAGTGG+TGG 0.517150 3.3:+69406487 MS.gene25697:CDS
ATCGGACTTTCTGGAAGCCA+TGG 0.517401 3.3:-69404852 None:intergenic
ATCAAGAGGACTATTCCAAA+GGG 0.519300 3.3:+69405408 MS.gene25697:CDS
GTCCCACCAAAACCAGCACC+AGG 0.522725 3.3:-69406694 None:intergenic
AGGAGGACCCTATTCTAGTA+GGG 0.525330 3.3:+69406717 MS.gene25697:CDS
AAAACTATCTTGTGTAAGGA+TGG 0.527528 3.3:-69406765 None:intergenic
CTAGAATCACTACCAAATGA+AGG 0.529653 3.3:-69406301 None:intergenic
GATTGATATGGATGGTACTC+AGG 0.532207 3.3:+69405899 MS.gene25697:CDS
AACTGATTCCGTTATCATGA+AGG 0.535063 3.3:+69405160 MS.gene25697:CDS
GGTGGAGATGATTTCAGTGG+TGG 0.538564 3.3:+69406460 MS.gene25697:CDS
GCCGCTATGGATCCTACATG+GGG 0.538686 3.3:+69406563 MS.gene25697:CDS
GGAGGCAGTGGTGGTGCTGG+TGG 0.540901 3.3:+69406496 MS.gene25697:CDS
AGAGGAGGAGAATCCTCATT+CGG 0.541881 3.3:+69406532 MS.gene25697:CDS
ACATCTTATAGGTCGCTTGG+AGG 0.543240 3.3:+69406400 MS.gene25697:CDS
ATGTCGATCCTTCATGATAA+CGG 0.543779 3.3:-69405168 None:intergenic
GGAGGTTATGAATCTGGCCC+TGG 0.544539 3.3:+69406676 MS.gene25697:CDS
TTCTAGTAGGGGAGGTTATG+GGG 0.546191 3.3:+69406729 MS.gene25697:CDS
GAATCCTCATTCGGTTACTC+TGG 0.546282 3.3:+69406541 MS.gene25697:CDS
CAAGCGACCTATAAGATGTA+GGG 0.548725 3.3:-69406396 None:intergenic
CCTACATCTTATAGGTCGCT+TGG 0.550258 3.3:+69406397 MS.gene25697:CDS
AGAACTTCTTCTCAAAGCAT+GGG 0.550482 3.3:+69405760 MS.gene25697:CDS
AACTACAAAACCAAATCCAC+GGG 0.550513 3.3:-69405829 None:intergenic
TCACATCATCAATGACAAGC+AGG 0.551218 3.3:+69405265 MS.gene25697:CDS
AGCGATTATGGTGGGTATGG+AGG 0.554528 3.3:+69406439 MS.gene25697:CDS
GTGGTGGGTTAGGCCCATAT+GGG 0.555497 3.3:+69406608 MS.gene25697:CDS
GGTAGGTTTAGCGATTATGG+TGG 0.558160 3.3:+69406430 MS.gene25697:CDS
TCATGCAGGAAAATCTTCAT+CGG 0.559658 3.3:+69404987 MS.gene25697:intron
GATAACCCTCACTCCGGCGA+CGG 0.564558 3.3:+69404872 MS.gene25697:CDS
TTAGGCCCATATGGGAGAGG+TGG 0.567019 3.3:+69406616 MS.gene25697:CDS
GGGTTAGGCCCATATGGGAG+AGG 0.567087 3.3:+69406613 MS.gene25697:CDS
TATGGTGGTCCAGGCACTGG+TGG 0.570069 3.3:+69406655 MS.gene25697:CDS
GCGGCCAGAGTAACCGAATG+AGG 0.570661 3.3:-69406545 None:intergenic
GTTGTAGGTTGAGATCAAGA+AGG 0.571670 3.3:+69406243 MS.gene25697:intron
CGCTATGGATCCTACATGGG+GGG 0.574927 3.3:+69406565 MS.gene25697:CDS
GATTATGGTGGGTATGGAGG+TGG 0.580479 3.3:+69406442 MS.gene25697:CDS
ATTCCAGCAACTGTATCTGA+AGG 0.581606 3.3:+69405480 MS.gene25697:CDS
AAACTACAAAACCAAATCCA+CGG 0.581859 3.3:-69405830 None:intergenic
TCTAGTAGGGGAGGTTATGG+GGG 0.582748 3.3:+69406730 MS.gene25697:CDS
CATATCAGGTTGAAATCAAG+AGG 0.589740 3.3:+69405394 MS.gene25697:intron
GTAGGTTTAGCGATTATGGT+GGG 0.596984 3.3:+69406431 MS.gene25697:CDS
TGCAGGAAAATCTTCATCGG+AGG 0.597403 3.3:+69404990 MS.gene25697:intron
ATTTGGGTTTATCACTTATG+CGG 0.598016 3.3:+69405208 MS.gene25697:CDS
TCTAGGGCACGTTCTTACAA+TGG 0.598638 3.3:+69406319 MS.gene25697:CDS
GTAGGGCCATAACCTCCAGC+AGG 0.600122 3.3:-69406379 None:intergenic
AATCAAGAGGACTATTCCAA+AGG 0.601418 3.3:+69405407 MS.gene25697:CDS
ATCAGCTAACAAATTATCAA+CGG 0.611456 3.3:-69405868 None:intergenic
AGGGCACGTTCTTACAATGG+CGG 0.614082 3.3:+69406322 MS.gene25697:CDS
CGACATACCGGTCGTCCTCG+AGG 0.619903 3.3:+69405185 MS.gene25697:CDS
GAAGTACTTTGAAAGGTATG+GGG 0.631008 3.3:+69405133 MS.gene25697:CDS
GGACCCTATTCTAGTAGGGG+AGG 0.635400 3.3:+69406721 MS.gene25697:CDS
TTAGCCAAAGATACAACCCT+AGG 0.645736 3.3:+69405014 MS.gene25697:CDS
CATACCACTAAGCGTTCCCG+TGG 0.650362 3.3:+69405813 MS.gene25697:CDS
GGTTATGCGGGCTATAGAGG+AGG 0.660221 3.3:+69406517 MS.gene25697:CDS
GGGAACATGATTGATATGGA+TGG 0.665118 3.3:+69405891 MS.gene25697:CDS
AAGTCCGATAACCCTCACTC+CGG 0.666720 3.3:+69404866 MS.gene25697:CDS
GGAGGACCCTATTCTAGTAG+GGG 0.666818 3.3:+69406718 MS.gene25697:CDS
CCGCTATGGATCCTACATGG+GGG 0.674316 3.3:+69406564 MS.gene25697:CDS
CACCACCATATCCACCACCA+AGG 0.678240 3.3:-69406588 None:intergenic
GGAGGTGGAGATGATTTCAG+TGG 0.679386 3.3:+69406457 MS.gene25697:CDS
TCCCACCAAAACCAGCACCA+GGG 0.688176 3.3:-69406693 None:intergenic
GTGATAAACCCAAATCCTCG+AGG 0.709143 3.3:-69405200 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAGTATTATTTATTTTAT+AGG + chr3.3:69405078-69405097 MS.gene25697:intron 10.0%
!! AGTAGTAATAATAATAATTG+CGG + chr3.3:69406056-69406075 MS.gene25697:intron 15.0%
!!! AAAACTAAGAAAATTTTTGT+TGG + chr3.3:69405453-69405472 MS.gene25697:CDS 15.0%
!! TTCTATTTATGAATGAATCT+AGG + chr3.3:69406205-69406224 MS.gene25697:intron 20.0%
!!! ATTCAGTTGAATTTTTGATT+TGG + chr3.3:69405325-69405344 MS.gene25697:intron 20.0%
!!! TAGTTTGGTTTGTTTATTTA+GGG + chr3.3:69405923-69405942 MS.gene25697:intron 20.0%
!!! TTAGTTTGGTTTGTTTATTT+AGG + chr3.3:69405922-69405941 MS.gene25697:intron 20.0%
!!! TTCAGTTGAATTTTTGATTT+GGG + chr3.3:69405326-69405345 MS.gene25697:intron 20.0%
! AATAGTGAAAAAGAGAAATC+GGG - chr3.3:69404953-69404972 None:intergenic 25.0%
! ATCAGCTAACAAATTATCAA+CGG - chr3.3:69405871-69405890 None:intergenic 25.0%
! GAATAGTGAAAAAGAGAAAT+CGG - chr3.3:69404954-69404973 None:intergenic 25.0%
! GATGAATTTGAAGATTTCTT+TGG - chr3.3:69406274-69406293 None:intergenic 25.0%
!! ACTAAGAAAATTTTTGTTGG+TGG + chr3.3:69405456-69405475 MS.gene25697:CDS 25.0%
!!! TAAATAGAACCTTTTTTCCT+TGG - chr3.3:69406194-69406213 None:intergenic 25.0%
!!! TTGATAATTTGTTAGCTGAT+GGG + chr3.3:69405871-69405890 MS.gene25697:CDS 25.0%
AAACTACAAAACCAAATCCA+CGG - chr3.3:69405833-69405852 None:intergenic 30.0%
ACAAAGTGAAACCGTAATTA+GGG - chr3.3:69405296-69405315 None:intergenic 30.0%
ATAGTGAAAAAGAGAAATCG+GGG - chr3.3:69404952-69404971 None:intergenic 30.0%
ATGAAAAGATGTTGATACCT+AGG - chr3.3:69405034-69405053 None:intergenic 30.0%
TGAAAAGATGTTGATACCTA+GGG - chr3.3:69405033-69405052 None:intergenic 30.0%
TGTTGTTGATCAAGTTATTC+AGG + chr3.3:69405238-69405257 MS.gene25697:CDS 30.0%
! AGGGTGTTTAAAATTGAAAC+TGG + chr3.3:69405942-69405961 MS.gene25697:intron 30.0%
! ATTTGGGTTTATCACTTATG+CGG + chr3.3:69405208-69405227 MS.gene25697:CDS 30.0%
! TCATCTTCTTTTCCTTCATT+TGG + chr3.3:69406289-69406308 MS.gene25697:CDS 30.0%
! TGAAGTACTTTGAAAGGTAT+GGG + chr3.3:69405132-69405151 MS.gene25697:CDS 30.0%
!! CTTTTGTGAAGTACTTTGAA+AGG + chr3.3:69405126-69405145 MS.gene25697:CDS 30.0%
!! GTTGATAATTTGTTAGCTGA+TGG + chr3.3:69405870-69405889 MS.gene25697:CDS 30.0%
!! TTCATTTGGTAGTGATTCTA+GGG + chr3.3:69406303-69406322 MS.gene25697:CDS 30.0%
!! TTGATTTGTCTGTTCATATC+AGG + chr3.3:69405380-69405399 MS.gene25697:intron 30.0%
!!! TGTAGTTTTTGATAGCGATA+AGG + chr3.3:69405845-69405864 MS.gene25697:CDS 30.0%
!!! TTTGTTTTCGAGTGAAAGTA+TGG + chr3.3:69405620-69405639 MS.gene25697:intron 30.0%
!!! TTTTTTCCTTGGTTTTGGTT+GGG - chr3.3:69406183-69406202 None:intergenic 30.0%
AAAGTGAGACATCACATTGA+TGG - chr3.3:69406096-69406115 None:intergenic 35.0%
AACTACAAAACCAAATCCAC+GGG - chr3.3:69405832-69405851 None:intergenic 35.0%
AAGAACTTCTTCTCAAAGCA+TGG + chr3.3:69405759-69405778 MS.gene25697:CDS 35.0%
AATCAAGAGGACTATTCCAA+AGG + chr3.3:69405407-69405426 MS.gene25697:CDS 35.0%
AGAACTTCTTCTCAAAGCAT+GGG + chr3.3:69405760-69405779 MS.gene25697:CDS 35.0%
AGCAGGTAAAACCCTAATTA+CGG + chr3.3:69405282-69405301 MS.gene25697:intron 35.0%
AGCTTATTGTATGCTTGTCT+TGG + chr3.3:69405715-69405734 MS.gene25697:intron 35.0%
ATCAAGAGGACTATTCCAAA+GGG + chr3.3:69405408-69405427 MS.gene25697:CDS 35.0%
ATGTCGATCCTTCATGATAA+CGG - chr3.3:69405171-69405190 None:intergenic 35.0%
CAACCAAAACCAAGGAAAAA+AGG + chr3.3:69406182-69406201 MS.gene25697:intron 35.0%
CACAAAGTGAAACCGTAATT+AGG - chr3.3:69405297-69405316 None:intergenic 35.0%
CTAGAATCACTACCAAATGA+AGG - chr3.3:69406304-69406323 None:intergenic 35.0%
GAACATAGGTTAGTGGAAAT+TGG + chr3.3:69405978-69405997 MS.gene25697:intron 35.0%
GCTTATTGTATGCTTGTCTT+GGG + chr3.3:69405716-69405735 MS.gene25697:intron 35.0%
TCATGCAGGAAAATCTTCAT+CGG + chr3.3:69404987-69405006 MS.gene25697:intron 35.0%
TGATGGGAACATGATTGATA+TGG + chr3.3:69405887-69405906 MS.gene25697:CDS 35.0%
! AACTGATTCCGTTATCATGA+AGG + chr3.3:69405160-69405179 MS.gene25697:CDS 35.0%
! GAAGTACTTTGAAAGGTATG+GGG + chr3.3:69405133-69405152 MS.gene25697:CDS 35.0%
! GTGAAGTACTTTGAAAGGTA+TGG + chr3.3:69405131-69405150 MS.gene25697:CDS 35.0%
! TTGACTCGATTCTTTGTTGT+AGG + chr3.3:69406228-69406247 MS.gene25697:intron 35.0%
!! CATATCAGGTTGAAATCAAG+AGG + chr3.3:69405394-69405413 MS.gene25697:intron 35.0%
!! CTCATTTTTCGTTTTCATGC+AGG + chr3.3:69404973-69404992 MS.gene25697:intron 35.0%
!! CTTCATTTGGTAGTGATTCT+AGG + chr3.3:69406302-69406321 MS.gene25697:CDS 35.0%
!!! CTTTTTTCCTTGGTTTTGGT+TGG - chr3.3:69406184-69406203 None:intergenic 35.0%
!!! GAACCTTTTTTCCTTGGTTT+TGG - chr3.3:69406188-69406207 None:intergenic 35.0%
AAACCTTCAGATACAGTTGC+TGG - chr3.3:69405486-69405505 None:intergenic 40.0%
ATAACCTCCCCTACTAGAAT+AGG - chr3.3:69406728-69406747 None:intergenic 40.0%
ATCATGAAGGATCGACATAC+CGG + chr3.3:69405173-69405192 MS.gene25697:CDS 40.0%
ATTCCAGCAACTGTATCTGA+AGG + chr3.3:69405480-69405499 MS.gene25697:CDS 40.0%
ATTCTAGTAGGGGAGGTTAT+GGG + chr3.3:69406728-69406747 MS.gene25697:CDS 40.0%
CAAGCGACCTATAAGATGTA+GGG - chr3.3:69406399-69406418 None:intergenic 40.0%
CTCAAAGCATGGGAATGTTA+TGG + chr3.3:69405770-69405789 MS.gene25697:CDS 40.0%
GATACCTAGGGTTGTATCTT+TGG - chr3.3:69405021-69405040 None:intergenic 40.0%
GATTGATATGGATGGTACTC+AGG + chr3.3:69405899-69405918 MS.gene25697:CDS 40.0%
GGGAACATGATTGATATGGA+TGG + chr3.3:69405891-69405910 MS.gene25697:CDS 40.0%
GTAGGTTTAGCGATTATGGT+GGG + chr3.3:69406431-69406450 MS.gene25697:CDS 40.0%
GTTATGGCCCTACATCTTAT+AGG + chr3.3:69406389-69406408 MS.gene25697:CDS 40.0%
TAACCTCCCCTACTAGAATA+GGG - chr3.3:69406727-69406746 None:intergenic 40.0%
TACTGCTGAACATAGGTTAG+TGG + chr3.3:69405971-69405990 MS.gene25697:intron 40.0%
TATTCTAGTAGGGGAGGTTA+TGG + chr3.3:69406727-69406746 MS.gene25697:CDS 40.0%
TCACATCATCAATGACAAGC+AGG + chr3.3:69405265-69405284 MS.gene25697:CDS 40.0%
TCACATTGATGGAAAGCAAC+AGG - chr3.3:69406085-69406104 None:intergenic 40.0%
TCAGACACTTGCAATAACAC+TGG - chr3.3:69406120-69406139 None:intergenic 40.0%
TTAGCCAAAGATACAACCCT+AGG + chr3.3:69405014-69405033 MS.gene25697:CDS 40.0%
TTGCAAGTGTCTGACATATG+TGG + chr3.3:69406126-69406145 MS.gene25697:intron 40.0%
TTTGACGTGCAAAGATGTTG+TGG + chr3.3:69406002-69406021 MS.gene25697:intron 40.0%
! GATGATTTCAGTGGTGGTTT+TGG + chr3.3:69406466-69406485 MS.gene25697:CDS 40.0%
! GGTGGATTTGACGATTCTTA+TGG + chr3.3:69406349-69406368 MS.gene25697:CDS 40.0%
! GTTGTAGGTTGAGATCAAGA+AGG + chr3.3:69406243-69406262 MS.gene25697:intron 40.0%
! TCTTATGGAAGTTTTCCTGC+TGG + chr3.3:69406364-69406383 MS.gene25697:CDS 40.0%
! TTAGTTTGAGATGCACCCTT+TGG - chr3.3:69405426-69405445 None:intergenic 40.0%
!! TTTAGCGATTATGGTGGGTA+TGG + chr3.3:69406436-69406455 MS.gene25697:CDS 40.0%
AAGTGTCTGACATATGTGGC+GGG + chr3.3:69406130-69406149 MS.gene25697:intron 45.0%
ACATCTTATAGGTCGCTTGG+AGG + chr3.3:69406400-69406419 MS.gene25697:CDS 45.0%
ACTGGTGGAGGTTATGAATC+TGG + chr3.3:69406670-69406689 MS.gene25697:CDS 45.0%
AGAGGAGGAGAATCCTCATT+CGG + chr3.3:69406532-69406551 MS.gene25697:CDS 45.0%
AGGAGGACCCTATTCTAGTA+GGG + chr3.3:69406717-69406736 MS.gene25697:CDS 45.0%
CAAGTGTCTGACATATGTGG+CGG + chr3.3:69406129-69406148 MS.gene25697:intron 45.0%
CCAAGCGACCTATAAGATGT+AGG - chr3.3:69406400-69406419 None:intergenic 45.0%
CCTACATCTTATAGGTCGCT+TGG + chr3.3:69406397-69406416 MS.gene25697:CDS 45.0%
GAATCCTCATTCGGTTACTC+TGG + chr3.3:69406541-69406560 MS.gene25697:CDS 45.0%
GGTAGGTTTAGCGATTATGG+TGG + chr3.3:69406430-69406449 MS.gene25697:CDS 45.0%
GGTGGTAGGTTTAGCGATTA+TGG + chr3.3:69406427-69406446 MS.gene25697:CDS 45.0%
GTGATAAACCCAAATCCTCG+AGG - chr3.3:69405203-69405222 None:intergenic 45.0%
GTGATGCTACTGCTGAACAT+AGG + chr3.3:69405964-69405983 MS.gene25697:intron 45.0%
TATCTGCCCAACCAAAACCA+AGG + chr3.3:69406174-69406193 MS.gene25697:intron 45.0%
TGCAGGAAAATCTTCATCGG+AGG + chr3.3:69404990-69405009 MS.gene25697:intron 45.0%
TTCTAGTAGGGGAGGTTATG+GGG + chr3.3:69406729-69406748 MS.gene25697:CDS 45.0%
! AGTTTTCCTGCTGGAGGTTA+TGG + chr3.3:69406373-69406392 MS.gene25697:CDS 45.0%
! CGTTCTTACAATGGCGGTTT+TGG + chr3.3:69406328-69406347 MS.gene25697:CDS 45.0%
! GGATGGTACTCAGGTTAGTT+TGG + chr3.3:69405908-69405927 MS.gene25697:intron 45.0%
! TATAGGTCGCTTGGAGGTTT+TGG + chr3.3:69406406-69406425 MS.gene25697:CDS 45.0%
! TATGGAAGTTTTCCTGCTGG+AGG + chr3.3:69406367-69406386 MS.gene25697:CDS 45.0%
! TCTAGGGCACGTTCTTACAA+TGG + chr3.3:69406319-69406338 MS.gene25697:CDS 45.0%
!! TCTTACAATGGCGGTTTTGG+TGG + chr3.3:69406331-69406350 MS.gene25697:CDS 45.0%
!!! AGTGGTGGTTTTGGAGGTTA+TGG + chr3.3:69406475-69406494 MS.gene25697:CDS 45.0%
!!! GATTTCAGTGGTGGTTTTGG+AGG + chr3.3:69406469-69406488 MS.gene25697:CDS 45.0%
AAATCCACGGGAACGCTTAG+TGG - chr3.3:69405820-69405839 None:intergenic 50.0%
AAGAGAGAAGAGAGCGAACC+CGG - chr3.3:69404906-69404925 None:intergenic 50.0%
AAGTCCGATAACCCTCACTC+CGG + chr3.3:69404866-69404885 MS.gene25697:CDS 50.0%
ACTAAGCGTTCCCGTGGATT+TGG + chr3.3:69405819-69405838 MS.gene25697:CDS 50.0%
AGGGCACGTTCTTACAATGG+CGG + chr3.3:69406322-69406341 MS.gene25697:CDS 50.0%
CAGGAGGACCCTATTCTAGT+AGG + chr3.3:69406716-69406735 MS.gene25697:CDS 50.0%
GGAGGACCCTATTCTAGTAG+GGG + chr3.3:69406718-69406737 MS.gene25697:CDS 50.0%
GGAGGTGGAGATGATTTCAG+TGG + chr3.3:69406457-69406476 MS.gene25697:CDS 50.0%
GGTGGAGATGATTTCAGTGG+TGG + chr3.3:69406460-69406479 MS.gene25697:CDS 50.0%
GTGAGGGTTATCGGACTTTC+TGG - chr3.3:69404864-69404883 None:intergenic 50.0%
TCTAGTAGGGGAGGTTATGG+GGG + chr3.3:69406730-69406749 MS.gene25697:CDS 50.0%
TTTGGAGGTTATGGAGGCAG+TGG + chr3.3:69406484-69406503 MS.gene25697:CDS 50.0%
!! AGCGATTATGGTGGGTATGG+AGG + chr3.3:69406439-69406458 MS.gene25697:CDS 50.0%
!! GATTATGGTGGGTATGGAGG+TGG + chr3.3:69406442-69406461 MS.gene25697:CDS 50.0%
!!! GGTGGTTTTGGAGGTTATGG+AGG + chr3.3:69406478-69406497 MS.gene25697:CDS 50.0%
AGAGAGAAGAGAGCGAACCC+GGG - chr3.3:69404905-69404924 None:intergenic 55.0%
CACCACCATATCCACCACCA+AGG - chr3.3:69406591-69406610 None:intergenic 55.0%
CACGGGAACGCTTAGTGGTA+TGG - chr3.3:69405815-69405834 None:intergenic 55.0%
CATACCACTAAGCGTTCCCG+TGG + chr3.3:69405813-69405832 MS.gene25697:CDS 55.0%
CCCCCATGTAGGATCCATAG+CGG - chr3.3:69406567-69406586 None:intergenic 55.0%
CCGCTATGGATCCTACATGG+GGG + chr3.3:69406564-69406583 MS.gene25697:CDS 55.0%
CGCTATGGATCCTACATGGG+GGG + chr3.3:69406565-69406584 MS.gene25697:CDS 55.0%
GCCGCTATGGATCCTACATG+GGG + chr3.3:69406563-69406582 MS.gene25697:CDS 55.0%
GGACCCTATTCTAGTAGGGG+AGG + chr3.3:69406721-69406740 MS.gene25697:CDS 55.0%
GGAGGTTATGAATCTGGCCC+TGG + chr3.3:69406676-69406695 MS.gene25697:CDS 55.0%
GGCCGCTATGGATCCTACAT+GGG + chr3.3:69406562-69406581 MS.gene25697:CDS 55.0%
TATGAATCTGGCCCTGGTGC+TGG + chr3.3:69406682-69406701 MS.gene25697:CDS 55.0%
TATGGCGGTTATGGTGGTCC+AGG + chr3.3:69406646-69406665 MS.gene25697:CDS 55.0%
TCATAACCTCCACCAGTGCC+TGG - chr3.3:69406667-69406686 None:intergenic 55.0%
TCCCACCAAAACCAGCACCA+GGG - chr3.3:69406696-69406715 None:intergenic 55.0%
TGGCCGCTATGGATCCTACA+TGG + chr3.3:69406561-69406580 MS.gene25697:CDS 55.0%
TTAGGCCCATATGGGAGAGG+TGG + chr3.3:69406616-69406635 MS.gene25697:CDS 55.0%
TTCGGTTACTCTGGCCGCTA+TGG + chr3.3:69406550-69406569 MS.gene25697:CDS 55.0%
! GGTGGTTATGCGGGCTATAG+AGG + chr3.3:69406514-69406533 MS.gene25697:CDS 55.0%
! GGTTATGCGGGCTATAGAGG+AGG + chr3.3:69406517-69406536 MS.gene25697:CDS 55.0%
!! AGGTCGCTTGGAGGTTTTGG+TGG + chr3.3:69406409-69406428 MS.gene25697:CDS 55.0%
!! CGCTTGGAGGTTTTGGTGGT+AGG + chr3.3:69406413-69406432 MS.gene25697:CDS 55.0%
!! CTTGGTGGTGGATATGGTGG+TGG + chr3.3:69406589-69406608 MS.gene25697:CDS 55.0%
!! GGATATGGTGGTGGTGGGTT+AGG + chr3.3:69406598-69406617 MS.gene25697:CDS 55.0%
!! GTGGTGGATATGGTGGTGGT+GGG + chr3.3:69406593-69406612 MS.gene25697:CDS 55.0%
!! GTGGTGGGTTAGGCCCATAT+GGG + chr3.3:69406608-69406627 MS.gene25697:CDS 55.0%
!! TGGTGGTGCTGGTGGTTATG+CGG + chr3.3:69406504-69406523 MS.gene25697:CDS 55.0%
ACCGGTCGTCCTCGAGGATT+TGG + chr3.3:69405191-69405210 MS.gene25697:CDS 60.0%
CCCAAATCCTCGAGGACGAC+CGG - chr3.3:69405195-69405214 None:intergenic 60.0%
CCCATATGGGAGAGGTGGTG+GGG + chr3.3:69406621-69406640 MS.gene25697:CDS 60.0%
CCCCACCACCTCTCCCATAT+GGG - chr3.3:69406624-69406643 None:intergenic 60.0%
CCGGTCGTCCTCGAGGATTT+GGG + chr3.3:69405192-69405211 MS.gene25697:CDS 60.0%
GATAACCCTCACTCCGGCGA+CGG + chr3.3:69404872-69404891 MS.gene25697:CDS 60.0%
GCCCATATGGGAGAGGTGGT+GGG + chr3.3:69406620-69406639 MS.gene25697:CDS 60.0%
GCGGCCAGAGTAACCGAATG+AGG - chr3.3:69406548-69406567 None:intergenic 60.0%
GGAGGTTATGGAGGCAGTGG+TGG + chr3.3:69406487-69406506 MS.gene25697:CDS 60.0%
GGGTTAGGCCCATATGGGAG+AGG + chr3.3:69406613-69406632 MS.gene25697:CDS 60.0%
GGTTATGGTGGTCCAGGCAC+TGG + chr3.3:69406652-69406671 MS.gene25697:CDS 60.0%
GTAGGGCCATAACCTCCAGC+AGG - chr3.3:69406382-69406401 None:intergenic 60.0%
GTCCCACCAAAACCAGCACC+AGG - chr3.3:69406697-69406716 None:intergenic 60.0%
GTCGCCGGAGTGAGGGTTAT+CGG - chr3.3:69404873-69404892 None:intergenic 60.0%
! CCATATGGGAGAGGTGGTGG+GGG + chr3.3:69406622-69406641 MS.gene25697:CDS 60.0%
! TATGGAGGCAGTGGTGGTGC+TGG + chr3.3:69406493-69406512 MS.gene25697:CDS 60.0%
!! GGCCTTGGTGGTGGATATGG+TGG + chr3.3:69406586-69406605 MS.gene25697:CDS 60.0%
!! GGTGGTGCTGGTGGTTATGC+GGG + chr3.3:69406505-69406524 MS.gene25697:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr3.3:69406592-69406611 MS.gene25697:CDS 60.0%
!! GGTGGTGGGTTAGGCCCATA+TGG + chr3.3:69406607-69406626 MS.gene25697:CDS 60.0%
!! TATGGTGGTCCAGGCACTGG+TGG + chr3.3:69406655-69406674 MS.gene25697:CDS 60.0%
!!! GCCCTGGTGCTGGTTTTGGT+GGG + chr3.3:69406692-69406711 MS.gene25697:CDS 60.0%
!!! GCTGGTTTTGGTGGGACAGG+AGG + chr3.3:69406700-69406719 MS.gene25697:CDS 60.0%
!!! GGTGCTGGTTTTGGTGGGAC+AGG + chr3.3:69406697-69406716 MS.gene25697:CDS 60.0%
!!! TCTGGCCCTGGTGCTGGTTT+TGG + chr3.3:69406688-69406707 MS.gene25697:CDS 60.0%
ACCACCAAGGCCCCCCATGT+AGG - chr3.3:69406578-69406597 None:intergenic 65.0%
CCCCCACCACCTCTCCCATA+TGG - chr3.3:69406625-69406644 None:intergenic 65.0%
CGACATACCGGTCGTCCTCG+AGG + chr3.3:69405185-69405204 MS.gene25697:CDS 65.0%
GAAGAGAGCGAACCCGGGAC+TGG - chr3.3:69404900-69404919 None:intergenic 65.0%
GGATCCTACATGGGGGGCCT+TGG + chr3.3:69406571-69406590 MS.gene25697:CDS 65.0%
GGCCCATATGGGAGAGGTGG+TGG + chr3.3:69406619-69406638 MS.gene25697:CDS 65.0%
GGGGGCTATGGCGGTTATGG+TGG + chr3.3:69406640-69406659 MS.gene25697:CDS 65.0%
GGTGGGGGCTATGGCGGTTA+TGG + chr3.3:69406637-69406656 MS.gene25697:CDS 65.0%
TACATGGGGGGCCTTGGTGG+TGG + chr3.3:69406577-69406596 MS.gene25697:CDS 65.0%
TCCTACATGGGGGGCCTTGG+TGG + chr3.3:69406574-69406593 MS.gene25697:CDS 65.0%
!! AGAGGTGGTGGGGGCTATGG+CGG + chr3.3:69406631-69406650 MS.gene25697:CDS 65.0%
!! GGGGGCCTTGGTGGTGGATA+TGG + chr3.3:69406583-69406602 MS.gene25697:CDS 65.0%
!!! GGCCCTGGTGCTGGTTTTGG+TGG + chr3.3:69406691-69406710 MS.gene25697:CDS 65.0%
! GGAGGCAGTGGTGGTGCTGG+TGG + chr3.3:69406496-69406515 MS.gene25697:CDS 70.0%
!! GGGAGAGGTGGTGGGGGCTA+TGG + chr3.3:69406628-69406647 MS.gene25697:CDS 70.0%
!! GGTGGTCCAGGCACTGGTGG+AGG + chr3.3:69406658-69406677 MS.gene25697:CDS 70.0%
TGGCGCCGTCGCCGGAGTGA+GGG - chr3.3:69404880-69404899 None:intergenic 75.0%
! CTGGCGCCGTCGCCGGAGTG+AGG - chr3.3:69404881-69404900 None:intergenic 80.0%
! TCCGGCGACGGCGCCAGTCC+CGG + chr3.3:69404884-69404903 MS.gene25697:CDS 80.0%
! CCGGCGACGGCGCCAGTCCC+GGG + chr3.3:69404885-69404904 MS.gene25697:CDS 85.0%
!! CCCGGGACTGGCGCCGTCGC+CGG - chr3.3:69404888-69404907 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 69404854 69406783 69404854 ID=MS.gene25697
chr3.3 mRNA 69404854 69406783 69404854 ID=MS.gene25697.t1;Parent=MS.gene25697
chr3.3 exon 69404854 69404906 69404854 ID=MS.gene25697.t1.exon1;Parent=MS.gene25697.t1
chr3.3 CDS 69404854 69404906 69404854 ID=cds.MS.gene25697.t1;Parent=MS.gene25697.t1
chr3.3 exon 69404995 69405035 69404995 ID=MS.gene25697.t1.exon2;Parent=MS.gene25697.t1
chr3.3 CDS 69404995 69405035 69404995 ID=cds.MS.gene25697.t1;Parent=MS.gene25697.t1
chr3.3 exon 69405123 69405286 69405123 ID=MS.gene25697.t1.exon3;Parent=MS.gene25697.t1
chr3.3 CDS 69405123 69405286 69405123 ID=cds.MS.gene25697.t1;Parent=MS.gene25697.t1
chr3.3 exon 69405402 69405501 69405402 ID=MS.gene25697.t1.exon4;Parent=MS.gene25697.t1
chr3.3 CDS 69405402 69405501 69405402 ID=cds.MS.gene25697.t1;Parent=MS.gene25697.t1
chr3.3 exon 69405751 69405920 69405751 ID=MS.gene25697.t1.exon5;Parent=MS.gene25697.t1
chr3.3 CDS 69405751 69405920 69405751 ID=cds.MS.gene25697.t1;Parent=MS.gene25697.t1
chr3.3 exon 69406250 69406783 69406250 ID=MS.gene25697.t1.exon6;Parent=MS.gene25697.t1
chr3.3 CDS 69406250 69406783 69406250 ID=cds.MS.gene25697.t1;Parent=MS.gene25697.t1
Gene Sequence

>MS.gene25697

ATGGCTTCCAGAAAGTCCGATAACCCTCACTCCGGCGACGGCGCCAGTCCCGGGTTCGCTCTCTTCTCTCTTCTTACACATTCAATTCGCTTCGTCCCCGATTTCTCTTTTTCACTATTCTCATTTTTCGTTTTCATGCAGGAAAATCTTCATCGGAGGTTTAGCCAAAGATACAACCCTAGGTATCAACATCTTTTCATTTTTTTGATATTTTTCTTTTAATTTTCAGTATTATTTATTTTATAGGTTTTGATATTTTGATTCTGCAGAGACTTTTGTGAAGTACTTTGAAAGGTATGGGGAGATAACTGATTCCGTTATCATGAAGGATCGACATACCGGTCGTCCTCGAGGATTTGGGTTTATCACTTATGCGGATGCTTCTGTTGTTGATCAAGTTATTCAGGAGAATCACATCATCAATGACAAGCAGGTAAAACCCTAATTACGGTTTCACTTTGTGTTTTGTAGATTCAGTTGAATTTTTGATTTGGGCTGTTTTCAAATGTTTACTTTGTTTTCATTGTTGATTTGTCTGTTCATATCAGGTTGAAATCAAGAGGACTATTCCAAAGGGTGCATCTCAAACTAATGACTTCAAAACTAAGAAAATTTTTGTTGGTGGCATTCCAGCAACTGTATCTGAAGGTTTGTCTTCAGCACTTATAATTTTTTTGATATAATCAATGTTCGTTGTGTGCTTTGATTTGTTTATATAACAATTAGTGTTTATTGTGTGTTTATACAAGTTTGTTAGTCTGTATTGTTTGTTTTCGAGTGAAAGTATGGTATTGTGTGTTAGTGAGCTTGTTAGTTTGTATTGTTTGTTTTCGAGTGAAAGTATGGTATTGTGTGTTATTGAGCTTATTGTATGCTTGTCTTGGGTGTGTGTTTTAGATGAGCTCAAGAACTTCTTCTCAAAGCATGGGAATGTTATGGAGCACGAGATCATACGCGACCATACCACTAAGCGTTCCCGTGGATTTGGTTTTGTAGTTTTTGATAGCGATAAGGCCGTTGATAATTTGTTAGCTGATGGGAACATGATTGATATGGATGGTACTCAGGTTAGTTTGGTTTGTTTATTTAGGGTGTTTAAAATTGAAACTGGTGATGCTACTGCTGAACATAGGTTAGTGGAAATTGGTTTTGACGTGCAAAGATGTTGTGGAATAATTGATAATAATAATAATAATAGTAGTAGTAGTAATAATAATAATTGCGGATTCCTGTTGCTTTCCATCAATGTGATGTCTCACTTTGCCAGTGTTATTGCAAGTGTCTGACATATGTGGCGGGCTAACTGCACATTTTATCATTTATCTGCCCAACCAAAACCAAGGAAAAAAGGTTCTATTTATGAATGAATCTAGGTTGACTCGATTCTTTGTTGTAGGTTGAGATCAAGAAGGCTGAACCAAAGAAATCTTCAAATTCATCTTCTTTTCCTTCATTTGGTAGTGATTCTAGGGCACGTTCTTACAATGGCGGTTTTGGTGGATTTGACGATTCTTATGGAAGTTTTCCTGCTGGAGGTTATGGCCCTACATCTTATAGGTCGCTTGGAGGTTTTGGTGGTAGGTTTAGCGATTATGGTGGGTATGGAGGTGGAGATGATTTCAGTGGTGGTTTTGGAGGTTATGGAGGCAGTGGTGGTGCTGGTGGTTATGCGGGCTATAGAGGAGGAGAATCCTCATTCGGTTACTCTGGCCGCTATGGATCCTACATGGGGGGCCTTGGTGGTGGATATGGTGGTGGTGGGTTAGGCCCATATGGGAGAGGTGGTGGGGGCTATGGCGGTTATGGTGGTCCAGGCACTGGTGGAGGTTATGAATCTGGCCCTGGTGCTGGTTTTGGTGGGACAGGAGGACCCTATTCTAGTAGGGGAGGTTATGGGGGCAGTAGTCGTTACCATCCTTACACAAGATAG
Protein sequence

>MS.gene25697.t1

MASRKSDNPHSGDGASPGKIFIGGLAKDTTLETFVKYFERYGEITDSVIMKDRHTGRPRGFGFITYADASVVDQVIQENHIINDKQVEIKRTIPKGASQTNDFKTKKIFVGGIPATVSEDELKNFFSKHGNVMEHEIIRDHTTKRSRGFGFVVFDSDKAVDNLLADGNMIDMDGTQVEIKKAEPKKSSNSSSFPSFGSDSRARSYNGGFGGFDDSYGSFPAGGYGPTSYRSLGGFGGRFSDYGGYGGGDDFSGGFGGYGGSGGAGGYAGYRGGESSFGYSGRYGSYMGGLGGGYGGGGLGPYGRGGGGYGGYGGPGTGGGYESGPGAGFGGTGGPYSSRGGYGGSSRYHPYTR