AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70470.t1 MTR_3g082650 97.167 353 9 1 1 353 1 352 0.0 652
MS.gene70470.t1 MTR_6g004810 65.534 206 68 2 11 216 38 240 7.10e-89 272
MS.gene70470.t1 MTR_3g055990 37.500 304 154 10 18 292 6 302 7.58e-50 174
MS.gene70470.t1 MTR_3g055990 37.500 304 154 10 18 292 6 302 7.63e-50 174
MS.gene70470.t1 MTR_2g026180 38.683 243 113 6 17 239 3 229 1.04e-49 173
MS.gene70470.t1 MTR_5g097580 38.776 294 153 10 18 288 6 295 1.04e-49 173
MS.gene70470.t1 MTR_5g097580 38.776 294 153 10 18 288 6 295 1.56e-49 173
MS.gene70470.t1 MTR_1g073690 36.508 189 101 2 18 188 6 193 1.63e-44 159
MS.gene70470.t1 MTR_3g103420 35.106 188 102 2 18 186 10 196 5.36e-38 139
MS.gene70470.t1 MTR_5g017280 32.143 168 112 1 19 186 5 170 4.94e-31 121
MS.gene70470.t1 MTR_5g017280 32.143 168 112 1 19 186 5 170 7.09e-31 121
MS.gene70470.t1 MTR_2g026180 35.057 174 77 6 86 239 2 159 1.78e-21 95.1
MS.gene70470.t1 MTR_2g026180 44.000 100 52 2 3 98 18 117 4.97e-20 90.9
MS.gene70470.t1 MTR_4g088010 31.304 230 129 9 19 226 77 299 5.20e-20 90.1
MS.gene70470.t1 MTR_4g104470 27.684 177 120 3 19 187 123 299 4.26e-18 84.0
MS.gene70470.t1 MTR_4g122650 29.752 242 129 9 9 226 71 295 2.58e-16 80.5
MS.gene70470.t1 MTR_3g027140 25.000 184 121 3 13 182 55 235 1.25e-13 70.1
MS.gene70470.t1 MTR_7g118120 29.050 179 113 6 19 192 105 274 5.45e-13 70.1
MS.gene70470.t1 MTR_7g107580 25.738 237 140 10 19 236 17 236 1.64e-12 68.6
MS.gene70470.t1 MTR_7g107580 25.738 237 140 10 19 236 17 236 1.79e-12 68.2
MS.gene70470.t1 MTR_7g107580 25.316 237 142 9 19 236 17 237 3.41e-12 67.8
MS.gene70470.t1 MTR_4g070190 50.847 59 29 0 19 77 9 67 5.23e-12 63.9
MS.gene70470.t1 MTR_4g070080 49.153 59 30 0 19 77 7 65 5.54e-12 62.8
MS.gene70470.t1 MTR_1g045510 29.114 158 101 3 37 187 132 285 1.01e-11 65.1
MS.gene70470.t1 MTR_8g024520 40.909 66 39 0 19 84 26 91 1.01e-11 65.1
MS.gene70470.t1 MTR_8g024520 40.909 66 39 0 19 84 26 91 1.03e-11 65.1
MS.gene70470.t1 MTR_4g070080 49.153 59 30 0 19 77 7 65 1.37e-11 62.8
MS.gene70470.t1 MTR_3g075500 23.333 180 127 3 18 186 109 288 1.44e-11 64.7
MS.gene70470.t1 MTR_7g117495 42.188 64 37 0 19 82 19 82 1.46e-11 64.3
MS.gene70470.t1 MTR_7g117495 42.188 64 37 0 19 82 19 82 1.83e-11 63.9
MS.gene70470.t1 MTR_4g035310 40.909 66 39 0 19 84 17 82 1.85e-11 64.3
MS.gene70470.t1 MTR_4g035310 40.909 66 39 0 19 84 17 82 1.96e-11 64.3
MS.gene70470.t1 MTR_3g116130 37.879 66 41 0 19 84 30 95 2.27e-11 63.9
MS.gene70470.t1 MTR_4g070140 45.000 60 33 0 19 78 9 68 2.54e-11 62.4
MS.gene70470.t1 MTR_4g052940 26.923 156 96 7 9 161 107 247 2.66e-11 65.1
MS.gene70470.t1 MTR_5g088220 25.000 160 112 2 19 170 166 325 3.81e-11 64.7
MS.gene70470.t1 MTR_1g064230 25.888 197 114 4 19 186 85 278 5.21e-11 63.2
MS.gene70470.t1 MTR_3g116130 37.879 66 41 0 19 84 30 95 6.70e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70470.t1 AT3G13224 59.189 370 122 10 1 353 1 358 3.40e-102 305
MS.gene70470.t1 AT3G13224 58.689 351 117 9 19 353 11 349 3.25e-95 287
MS.gene70470.t1 AT3G13224 71.739 184 49 2 1 181 1 184 4.88e-89 267
MS.gene70470.t1 AT5G40490 67.172 198 60 4 8 202 31 226 1.49e-81 254
MS.gene70470.t1 AT5G40490 65.027 183 60 3 22 202 29 209 8.06e-74 234
MS.gene70470.t1 AT1G17640 51.892 185 86 1 17 198 65 249 2.74e-66 214
MS.gene70470.t1 AT1G17640 51.892 185 86 1 17 198 65 249 2.74e-66 214
MS.gene70470.t1 AT1G17640 51.892 185 86 1 17 198 65 249 2.74e-66 214
MS.gene70470.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene70470.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene70470.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene70470.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene70470.t1 AT5G55550 40.239 251 118 5 18 246 6 246 2.39e-49 172
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 5.35e-19 88.2
MS.gene70470.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene70470.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene70470.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene70470.t1 AT5G55550 38.112 286 142 7 18 280 6 279 4.19e-49 171
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 101 198 6.68e-19 87.8
MS.gene70470.t1 AT4G26650 40.933 193 94 2 18 191 12 203 1.26e-47 167
MS.gene70470.t1 AT4G26650 44.444 99 54 1 11 108 112 210 3.89e-19 88.6
MS.gene70470.t1 AT4G26650 40.933 193 94 2 18 191 15 206 1.56e-47 167
MS.gene70470.t1 AT4G26650 44.444 99 54 1 11 108 115 213 3.87e-19 88.6
MS.gene70470.t1 AT4G14300 40.107 187 95 2 18 188 6 191 5.05e-46 162
MS.gene70470.t1 AT4G14300 40.107 187 95 2 18 188 6 191 5.05e-46 162
MS.gene70470.t1 AT2G33410 40.642 187 94 3 18 188 6 191 5.05e-45 159
MS.gene70470.t1 AT2G33410 46.939 98 48 2 2 98 97 191 4.42e-19 88.2
MS.gene70470.t1 AT3G07810 35.938 256 139 6 18 256 6 253 5.94e-45 161
MS.gene70470.t1 AT3G07810 35.938 256 139 6 18 256 6 253 6.48e-45 160
MS.gene70470.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.18e-42 153
MS.gene70470.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.85e-13 70.9
MS.gene70470.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.18e-42 153
MS.gene70470.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.85e-13 70.9
MS.gene70470.t1 AT5G47620 37.255 255 132 8 19 251 7 255 1.18e-42 153
MS.gene70470.t1 AT5G47620 36.522 115 65 2 102 210 2 114 2.85e-13 70.9
MS.gene70470.t1 AT5G55550 33.033 333 141 8 18 280 6 326 1.74e-41 151
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 148 245 9.43e-19 87.8
MS.gene70470.t1 AT5G55550 33.033 333 141 8 18 280 6 326 2.82e-41 151
MS.gene70470.t1 AT5G55550 43.878 98 54 1 9 105 148 245 1.13e-18 87.4
MS.gene70470.t1 AT5G47620 34.296 277 132 9 19 251 7 277 9.60e-38 140
MS.gene70470.t1 AT1G58470 31.624 234 119 3 19 226 7 225 5.15e-34 129
MS.gene70470.t1 AT1G58470 35.185 108 68 1 102 209 2 107 4.17e-11 63.9
MS.gene70470.t1 AT1G58470 31.624 234 119 3 19 226 7 225 5.15e-34 129
MS.gene70470.t1 AT1G58470 35.185 108 68 1 102 209 2 107 4.17e-11 63.9
MS.gene70470.t1 AT4G36960 31.148 183 124 1 19 201 4 184 3.36e-31 121
MS.gene70470.t1 AT4G36960 31.148 183 124 1 19 201 4 184 3.36e-31 121
MS.gene70470.t1 AT5G47620 45.455 99 45 2 19 108 47 145 8.92e-20 89.7
MS.gene70470.t1 AT3G15010 30.952 168 111 2 19 182 76 242 4.55e-19 88.2
MS.gene70470.t1 AT3G15010 30.952 168 111 2 19 182 76 242 4.55e-19 88.2
MS.gene70470.t1 AT5G50250 27.011 174 121 2 18 185 113 286 3.50e-18 84.0
MS.gene70470.t1 AT4G24770 26.425 193 131 5 2 186 137 326 1.87e-17 82.4
MS.gene70470.t1 AT4G24770 26.425 193 131 5 2 186 137 326 1.87e-17 82.4
MS.gene70470.t1 AT4G00830 32.044 181 105 7 19 192 117 286 1.88e-17 84.0
MS.gene70470.t1 AT4G00830 24.597 248 150 9 18 255 196 416 5.50e-11 63.9
MS.gene70470.t1 AT4G00830 32.044 181 105 7 19 192 117 286 1.88e-17 84.0
MS.gene70470.t1 AT4G00830 24.597 248 150 9 18 255 196 416 5.50e-11 63.9
MS.gene70470.t1 AT4G00830 32.044 181 105 7 19 192 117 286 1.88e-17 84.0
MS.gene70470.t1 AT4G00830 24.597 248 150 9 18 255 196 416 5.50e-11 63.9
MS.gene70470.t1 AT4G00830 31.111 180 108 6 19 192 117 286 4.27e-17 82.8
MS.gene70470.t1 AT4G00830 24.597 248 150 9 18 255 196 416 4.31e-11 64.3
MS.gene70470.t1 AT3G52150 27.136 199 124 5 2 182 58 253 2.74e-15 75.1
MS.gene70470.t1 AT3G52150 27.136 199 124 5 2 182 58 253 2.74e-15 75.1
MS.gene70470.t1 AT3G26420 38.750 80 49 0 107 186 8 87 3.07e-15 74.7
MS.gene70470.t1 AT2G37220 25.521 192 118 2 19 185 92 283 4.30e-15 75.1
MS.gene70470.t1 AT5G04280 43.038 79 43 1 19 95 8 86 7.12e-15 74.7
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT3G56860 26.685 356 201 12 19 343 141 467 2.68e-14 74.3
MS.gene70470.t1 AT4G00830 29.189 185 97 7 19 192 117 278 4.51e-14 73.6
MS.gene70470.t1 AT4G00830 29.189 185 97 7 19 192 117 278 4.51e-14 73.6
MS.gene70470.t1 AT1G03457 28.959 221 132 9 19 225 13 222 8.90e-14 72.4
MS.gene70470.t1 AT4G16280 27.907 301 166 13 13 295 115 382 1.10e-13 72.8
MS.gene70470.t1 AT4G16280 27.907 301 166 13 13 295 115 382 1.29e-13 72.4
MS.gene70470.t1 AT4G16280 27.907 301 166 13 13 295 40 307 1.30e-13 72.4
MS.gene70470.t1 AT1G60650 41.379 87 49 1 11 95 5 91 2.57e-13 70.1
MS.gene70470.t1 AT1G60650 41.379 87 49 1 11 95 5 91 2.57e-13 70.1
MS.gene70470.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.51e-13 70.1
MS.gene70470.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.51e-13 70.1
MS.gene70470.t1 AT2G41060 27.011 348 202 14 19 339 129 451 6.51e-13 70.1
MS.gene70470.t1 AT2G46780 40.909 66 39 0 19 84 23 88 1.38e-12 68.2
MS.gene70470.t1 AT2G46780 40.909 66 39 0 19 84 23 88 1.38e-12 68.2
MS.gene70470.t1 AT4G03110 23.661 224 139 8 19 226 19 226 1.58e-12 68.6
MS.gene70470.t1 AT1G03457 27.826 230 132 9 19 225 13 231 1.58e-12 68.6
MS.gene70470.t1 AT4G03110 23.661 224 139 8 19 226 19 226 4.21e-12 67.4
MS.gene70470.t1 AT4G03110 23.661 224 139 8 19 226 19 226 4.58e-12 67.4
MS.gene70470.t1 AT4G39260 34.177 79 52 0 107 185 7 85 8.59e-12 61.2
MS.gene70470.t1 AT4G39260 36.585 82 48 2 19 100 7 84 8.84e-11 58.5
MS.gene70470.t1 AT1G76460 40.909 66 39 0 19 84 25 90 9.79e-12 65.5
MS.gene70470.t1 AT1G71770 25.521 192 118 7 4 177 223 407 1.39e-11 66.2
MS.gene70470.t1 AT1G71770 25.521 192 118 7 4 177 223 407 1.39e-11 66.2
MS.gene70470.t1 AT1G11650 25.571 219 125 5 20 217 157 358 1.90e-11 65.1
MS.gene70470.t1 AT3G23830 34.066 91 60 0 103 193 32 122 7.25e-11 60.1
MS.gene70470.t1 AT3G23830 34.066 91 60 0 103 193 32 122 7.25e-11 60.1
MS.gene70470.t1 AT5G59865 39.506 81 49 0 107 187 27 107 7.80e-11 59.3
MS.gene70470.t1 AT5G61030 30.769 104 70 1 84 185 16 119 8.72e-11 62.8
MS.gene70470.t1 AT5G61030 30.769 104 70 1 84 185 16 119 8.72e-11 62.8

Find 130 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGGGTTATCGGACTTTC+TGG 0.192489 3.1:+66645842 None:intergenic
CTTCATTTGGTAGTGATTCT+AGG 0.203855 3.1:-66644404 MS.gene70470:CDS
ACCGGTCGTCCTCGAGGATT+TGG 0.205607 3.1:-66645512 MS.gene70470:CDS
CGTTCTTACAATGGCGGTTT+TGG 0.224937 3.1:-66644378 MS.gene70470:CDS
TATACCTAGGGTTGTATCTT+TGG 0.228735 3.1:+66645685 None:intergenic
TCTGGCCCTGGTGCTGGTTT+TGG 0.239203 3.1:-66644018 MS.gene70470:CDS
TGTTGTTGATCAAGTTATTC+AGG 0.257520 3.1:-66645465 MS.gene70470:CDS
TATAGGTCGCTTGGAGGTTT+TGG 0.267096 3.1:-66644300 MS.gene70470:CDS
GGTGGTAGGTTTAGCGATTA+TGG 0.272361 3.1:-66644279 MS.gene70470:CDS
GATGAATTTGAAGATTTCTT+TGG 0.276198 3.1:+66644435 None:intergenic
GGCCCATATGGGAGAGGTGG+TGG 0.277336 3.1:-66644087 MS.gene70470:CDS
GATGATTTCAGTGGTGGTTT+TGG 0.287960 3.1:-66644240 MS.gene70470:CDS
GGATATGGTGGTGGTGGGTT+AGG 0.316266 3.1:-66644108 MS.gene70470:CDS
TCCGGCGACGGCGCCAGTCC+CGG 0.317544 3.1:-66645819 MS.gene70470:CDS
CCCATATGGGAGAGGTGGTG+GGG 0.320586 3.1:-66644085 MS.gene70470:CDS
TATTCTAGTAGGGGAGGTTA+TGG 0.322189 3.1:-66643979 MS.gene70470:CDS
GGTGGATTTGACGATTCTTA+TGG 0.325447 3.1:-66644357 MS.gene70470:CDS
GGTGGTGGGTTAGGCCCATA+TGG 0.328329 3.1:-66644099 MS.gene70470:CDS
ACTAAGCGTTCCCGTGGATT+TGG 0.349095 3.1:-66644884 MS.gene70470:CDS
ATTCTAGTAGGGGAGGTTAT+GGG 0.359170 3.1:-66643978 MS.gene70470:CDS
GGCCTTGGTGGTGGATATGG+TGG 0.362798 3.1:-66644120 MS.gene70470:CDS
GTGAAGTACTTTGAAAGGTA+TGG 0.367146 3.1:-66645572 MS.gene70470:CDS
CCGGTCGTCCTCGAGGATTT+GGG 0.373418 3.1:-66645511 MS.gene70470:CDS
GTTATGGCCCTACATCTTAT+AGG 0.373426 3.1:-66644317 MS.gene70470:CDS
CCGGCGACGGCGCCAGTCCC+GGG 0.373625 3.1:-66645818 MS.gene70470:intron
ATAACCTCCCCTACTAGAAT+AGG 0.380687 3.1:+66643981 None:intergenic
GTCGCCGGAGTGAGGGTTAT+CGG 0.392798 3.1:+66645833 None:intergenic
TATGGAGGCAGTGGTGGTGC+TGG 0.406140 3.1:-66644213 MS.gene70470:CDS
TTCATTTGGTAGTGATTCTA+GGG 0.412071 3.1:-66644403 MS.gene70470:CDS
CTTGGTGGTGGATATGGTGG+TGG 0.412985 3.1:-66644117 MS.gene70470:CDS
TTAGTTTGAGATGCACCCTT+TGG 0.413443 3.1:+66645280 None:intergenic
TGATGGGAACATGATTGATA+TGG 0.416351 3.1:-66644816 MS.gene70470:CDS
TATGAATCTGGCCCTGGTGC+TGG 0.417018 3.1:-66644024 MS.gene70470:CDS
CTCAAAGCATGGGAATGTTA+TGG 0.425693 3.1:-66644933 MS.gene70470:CDS
GGTTATGGTGGTCCAGGCAC+TGG 0.434215 3.1:-66644054 MS.gene70470:CDS
TGAAGTACTTTGAAAGGTAT+GGG 0.436031 3.1:-66645571 MS.gene70470:CDS
CCCGGGACTGGCGCCGTCGC+CGG 0.436702 3.1:+66645818 None:intergenic
GCCCATATGGGAGAGGTGGT+GGG 0.438600 3.1:-66644086 MS.gene70470:CDS
ACTGGTGGAGGTTATGAATC+TGG 0.439273 3.1:-66644036 MS.gene70470:CDS
CCATATGGGAGAGGTGGTGG+GGG 0.441700 3.1:-66644084 MS.gene70470:CDS
CACGGGAACGCTTAGTGGTA+TGG 0.442562 3.1:+66644891 None:intergenic
TTCGGTTACTCTGGCCGCTA+TGG 0.446589 3.1:-66644156 MS.gene70470:CDS
GAAGAGAGCGAACCCGGGAC+TGG 0.447616 3.1:+66645806 None:intergenic
GGTGGTGCTGGTGGTTATGC+GGG 0.451484 3.1:-66644201 MS.gene70470:CDS
TTTGGAGGTTATGGAGGCAG+TGG 0.453260 3.1:-66644222 MS.gene70470:CDS
GGTGGTGGATATGGTGGTGG+TGG 0.458107 3.1:-66644114 MS.gene70470:CDS
CCCCACCACCTCTCCCATAT+GGG 0.460579 3.1:+66644085 None:intergenic
CAGGAGGACCCTATTCTAGT+AGG 0.461637 3.1:-66643990 MS.gene70470:CDS
CCCAAATCCTCGAGGACGAC+CGG 0.461844 3.1:+66645511 None:intergenic
AAATCCACGGGAACGCTTAG+TGG 0.462420 3.1:+66644886 None:intergenic
GTGGTGGATATGGTGGTGGT+GGG 0.463417 3.1:-66644113 MS.gene70470:CDS
GGTGGTCCAGGCACTGGTGG+AGG 0.471128 3.1:-66644048 MS.gene70470:CDS
TCATAAAACTATCTTGTGTA+AGG 0.471625 3.1:+66643937 None:intergenic
TAACCTCCCCTACTAGAATA+GGG 0.473608 3.1:+66643982 None:intergenic
GGCCGCTATGGATCCTACAT+GGG 0.474978 3.1:-66644144 MS.gene70470:CDS
AAACCTTCAGATACAGTTGC+TGG 0.484805 3.1:+66645220 None:intergenic
TTTAGCGATTATGGTGGGTA+TGG 0.487404 3.1:-66644270 MS.gene70470:CDS
GTTGATAATTTGTTAGCTGA+TGG 0.487526 3.1:-66644833 MS.gene70470:CDS
GGTGGTTATGCGGGCTATAG+AGG 0.488404 3.1:-66644192 MS.gene70470:CDS
CTGGCGCCGTCGCCGGAGTG+AGG 0.489242 3.1:+66645825 None:intergenic
TGGCCGCTATGGATCCTACA+TGG 0.493274 3.1:-66644145 MS.gene70470:CDS
TCATAACCTCCACCAGTGCC+TGG 0.495641 3.1:+66644042 None:intergenic
AAGAACTTCTTCTCAAAGCA+TGG 0.497829 3.1:-66644944 MS.gene70470:CDS
TATGGCGGTTATGGTGGTCC+AGG 0.499140 3.1:-66644060 MS.gene70470:CDS
CCAAGCGACCTATAAGATGT+AGG 0.501589 3.1:+66644309 None:intergenic
TGGCGCCGTCGCCGGAGTGA+GGG 0.503462 3.1:+66645826 None:intergenic
ATCATGAAGGATCGACATAC+CGG 0.510399 3.1:-66645530 MS.gene70470:CDS
TTGATAATTTGTTAGCTGAT+GGG 0.512546 3.1:-66644832 MS.gene70470:CDS
TGGTGGTGCTGGTGGTTATG+CGG 0.514299 3.1:-66644202 MS.gene70470:CDS
GGAGGTTATGGAGGCAGTGG+TGG 0.517150 3.1:-66644219 MS.gene70470:CDS
ATCGGACTTTCTGGAAGCCA+TGG 0.517401 3.1:+66645851 None:intergenic
ATCAAGAGGACTATTCCAAA+GGG 0.519300 3.1:-66645295 MS.gene70470:CDS
GTCCCACCAAAACCAGCACC+AGG 0.522725 3.1:+66644012 None:intergenic
AGGAGGACCCTATTCTAGTA+GGG 0.525330 3.1:-66643989 MS.gene70470:CDS
AAAACTATCTTGTGTAAGGA+TGG 0.527528 3.1:+66643941 None:intergenic
CTAGAATCACTACCAAATGA+AGG 0.529653 3.1:+66644405 None:intergenic
GATTGATATGGATGGTACTC+AGG 0.532207 3.1:-66644804 MS.gene70470:intron
AACTGATTCCGTTATCATGA+AGG 0.535063 3.1:-66645543 MS.gene70470:CDS
GGTGGAGATGATTTCAGTGG+TGG 0.538564 3.1:-66644246 MS.gene70470:CDS
GCCGCTATGGATCCTACATG+GGG 0.538686 3.1:-66644143 MS.gene70470:CDS
GGAGGCAGTGGTGGTGCTGG+TGG 0.540901 3.1:-66644210 MS.gene70470:CDS
AGAGGAGGAGAATCCTCATT+CGG 0.541881 3.1:-66644174 MS.gene70470:CDS
ACATCTTATAGGTCGCTTGG+AGG 0.543240 3.1:-66644306 MS.gene70470:CDS
ATGTCGATCCTTCATGATAA+CGG 0.543779 3.1:+66645535 None:intergenic
GGAGGTTATGAATCTGGCCC+TGG 0.544539 3.1:-66644030 MS.gene70470:CDS
TTCTAGTAGGGGAGGTTATG+GGG 0.546191 3.1:-66643977 MS.gene70470:CDS
GAATCCTCATTCGGTTACTC+TGG 0.546282 3.1:-66644165 MS.gene70470:CDS
CAAGCGACCTATAAGATGTA+GGG 0.548725 3.1:+66644310 None:intergenic
CCTACATCTTATAGGTCGCT+TGG 0.550258 3.1:-66644309 MS.gene70470:CDS
AGAACTTCTTCTCAAAGCAT+GGG 0.550482 3.1:-66644943 MS.gene70470:CDS
AACTACAAAACCAAATCCAC+GGG 0.550513 3.1:+66644874 None:intergenic
TCACATCATCAATGACAAGC+AGG 0.551218 3.1:-66645438 MS.gene70470:intron
AGCGATTATGGTGGGTATGG+AGG 0.554528 3.1:-66644267 MS.gene70470:CDS
GTGGTGGGTTAGGCCCATAT+GGG 0.555497 3.1:-66644098 MS.gene70470:CDS
GGTAGGTTTAGCGATTATGG+TGG 0.558160 3.1:-66644276 MS.gene70470:CDS
TCATGCAGGAAAATCTTCAT+CGG 0.559658 3.1:-66645716 MS.gene70470:intron
GATAACCCTCACTCCGGCGA+CGG 0.564558 3.1:-66645831 MS.gene70470:CDS
TTAGGCCCATATGGGAGAGG+TGG 0.567019 3.1:-66644090 MS.gene70470:CDS
GGGTTAGGCCCATATGGGAG+AGG 0.567087 3.1:-66644093 MS.gene70470:CDS
TATGGTGGTCCAGGCACTGG+TGG 0.570069 3.1:-66644051 MS.gene70470:CDS
GCGGCCAGAGTAACCGAATG+AGG 0.570661 3.1:+66644161 None:intergenic
GTTGTAGGTTGAGATCAAGA+AGG 0.571670 3.1:-66644463 MS.gene70470:intron
CGCTATGGATCCTACATGGG+GGG 0.574927 3.1:-66644141 MS.gene70470:CDS
GATTATGGTGGGTATGGAGG+TGG 0.580479 3.1:-66644264 MS.gene70470:CDS
ATTCCAGCAACTGTATCTGA+AGG 0.581606 3.1:-66645223 MS.gene70470:intron
AAACTACAAAACCAAATCCA+CGG 0.581859 3.1:+66644873 None:intergenic
TCTAGTAGGGGAGGTTATGG+GGG 0.582748 3.1:-66643976 MS.gene70470:CDS
CATATCAGGTTGAAATCAAG+AGG 0.589740 3.1:-66645309 MS.gene70470:intron
GTAGGTTTAGCGATTATGGT+GGG 0.596984 3.1:-66644275 MS.gene70470:CDS
TGCAGGAAAATCTTCATCGG+AGG 0.597403 3.1:-66645713 MS.gene70470:intron
ATTTGGGTTTATCACTTATG+CGG 0.598016 3.1:-66645495 MS.gene70470:CDS
TCTAGGGCACGTTCTTACAA+TGG 0.598638 3.1:-66644387 MS.gene70470:CDS
GTAGGGCCATAACCTCCAGC+AGG 0.600122 3.1:+66644327 None:intergenic
AATCAAGAGGACTATTCCAA+AGG 0.601418 3.1:-66645296 MS.gene70470:CDS
ATCAGCTAACAAATTATCAA+CGG 0.611456 3.1:+66644835 None:intergenic
AGGGCACGTTCTTACAATGG+CGG 0.614082 3.1:-66644384 MS.gene70470:CDS
CGACATACCGGTCGTCCTCG+AGG 0.619903 3.1:-66645518 MS.gene70470:CDS
GAAGTACTTTGAAAGGTATG+GGG 0.631008 3.1:-66645570 MS.gene70470:CDS
GGACCCTATTCTAGTAGGGG+AGG 0.635400 3.1:-66643985 MS.gene70470:CDS
TTAGCCAAAGATACAACCCT+AGG 0.645736 3.1:-66645689 MS.gene70470:intron
CATACCACTAAGCGTTCCCG+TGG 0.650362 3.1:-66644890 MS.gene70470:CDS
GGTTATGCGGGCTATAGAGG+AGG 0.660221 3.1:-66644189 MS.gene70470:CDS
GGGAACATGATTGATATGGA+TGG 0.665118 3.1:-66644812 MS.gene70470:CDS
AAGTCCGATAACCCTCACTC+CGG 0.666720 3.1:-66645837 MS.gene70470:CDS
GGAGGACCCTATTCTAGTAG+GGG 0.666818 3.1:-66643988 MS.gene70470:CDS
CCGCTATGGATCCTACATGG+GGG 0.674316 3.1:-66644142 MS.gene70470:CDS
CACCACCATATCCACCACCA+AGG 0.678240 3.1:+66644118 None:intergenic
GGAGGTGGAGATGATTTCAG+TGG 0.679386 3.1:-66644249 MS.gene70470:CDS
TCCCACCAAAACCAGCACCA+GGG 0.688176 3.1:+66644013 None:intergenic
GTGATAAACCCAAATCCTCG+AGG 0.709143 3.1:+66645503 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAGTATTATTTATTTTAT+AGG - chr3.1:66644169-66644188 MS.gene70470:CDS 10.0%
!! AGTAGTAATAATAATAATTG+CGG - chr3.1:66645144-66645163 MS.gene70470:intron 15.0%
!! TTCTATTTATGAATGAATCT+AGG - chr3.1:66645293-66645312 MS.gene70470:CDS 20.0%
!!! AAAACTAAGAAGATTTTTGT+TGG - chr3.1:66644544-66644563 MS.gene70470:intron 20.0%
!!! ATTCAGTTGAATTTTTGATT+TGG - chr3.1:66644416-66644435 MS.gene70470:CDS 20.0%
!!! TAGTTTGGTTTGTTTATTTA+GGG - chr3.1:66645014-66645033 MS.gene70470:intron 20.0%
!!! TTAGTTTGGTTTGTTTATTT+AGG - chr3.1:66645013-66645032 MS.gene70470:intron 20.0%
!!! TTCAGTTGAATTTTTGATTT+GGG - chr3.1:66644417-66644436 MS.gene70470:CDS 20.0%
! AATAGTGAAAAAGAGAAATC+GGG + chr3.1:66644044-66644063 None:intergenic 25.0%
! ATCAGCTAACAAATTATCAA+CGG + chr3.1:66644962-66644981 None:intergenic 25.0%
! ATGAAAAGATGTTTATACCT+AGG + chr3.1:66644125-66644144 None:intergenic 25.0%
! GAATAGTGAAAAAGAGAAAT+CGG + chr3.1:66644045-66644064 None:intergenic 25.0%
! GATGAATTTGAAGATTTCTT+TGG + chr3.1:66645362-66645381 None:intergenic 25.0%
! TGAAAAGATGTTTATACCTA+GGG + chr3.1:66644124-66644143 None:intergenic 25.0%
!!! TAAATAGAACCTTTTTTCCT+TGG + chr3.1:66645282-66645301 None:intergenic 25.0%
!!! TTGATAATTTGTTAGCTGAT+GGG - chr3.1:66644962-66644981 MS.gene70470:intron 25.0%
AAACTACAAAACCAAATCCA+CGG + chr3.1:66644924-66644943 None:intergenic 30.0%
ACAAAGTGAAACCGTAATTA+GGG + chr3.1:66644387-66644406 None:intergenic 30.0%
AGCTTATTGTATGCTTATCT+TGG - chr3.1:66644806-66644825 MS.gene70470:CDS 30.0%
ATAGTGAAAAAGAGAAATCG+GGG + chr3.1:66644043-66644062 None:intergenic 30.0%
GCTTATTGTATGCTTATCTT+GGG - chr3.1:66644807-66644826 MS.gene70470:CDS 30.0%
TGTTGTTGATCAAGTTATTC+AGG - chr3.1:66644329-66644348 MS.gene70470:CDS 30.0%
! ACTAAGAAGATTTTTGTTGG+TGG - chr3.1:66644547-66644566 MS.gene70470:intron 30.0%
! AGGGTGTTTAAAATTGAAAC+TGG - chr3.1:66645033-66645052 MS.gene70470:intron 30.0%
! ATTTGGGTTTATCACTTATG+CGG - chr3.1:66644299-66644318 MS.gene70470:CDS 30.0%
! TCATCTTCTTTTCCTTCATT+TGG - chr3.1:66645377-66645396 MS.gene70470:intron 30.0%
! TGAAGTACTTTGAAAGGTAT+GGG - chr3.1:66644223-66644242 MS.gene70470:CDS 30.0%
!! CTTTTGTGAAGTACTTTGAA+AGG - chr3.1:66644217-66644236 MS.gene70470:CDS 30.0%
!! GTTGATAATTTGTTAGCTGA+TGG - chr3.1:66644961-66644980 MS.gene70470:intron 30.0%
!! TTCATTTGGTAGTGATTCTA+GGG - chr3.1:66645391-66645410 MS.gene70470:intron 30.0%
!! TTGATTTGTCTGTTCATATC+AGG - chr3.1:66644471-66644490 MS.gene70470:intron 30.0%
!!! TGTAGTTTTTGATAGCGATA+AGG - chr3.1:66644936-66644955 MS.gene70470:CDS 30.0%
!!! TTTGTTTTCGAGTGAAAGTA+TGG - chr3.1:66644711-66644730 MS.gene70470:intron 30.0%
!!! TTTTTTCCTTGGTTTTGGTT+GGG + chr3.1:66645271-66645290 None:intergenic 30.0%
AAAGTGAGACATCACATTGA+TGG + chr3.1:66645184-66645203 None:intergenic 35.0%
AACTACAAAACCAAATCCAC+GGG + chr3.1:66644923-66644942 None:intergenic 35.0%
AAGAACTTCTTCTCAAAGCA+TGG - chr3.1:66644850-66644869 MS.gene70470:CDS 35.0%
AATCAAGAGGACTATTCCAA+AGG - chr3.1:66644498-66644517 MS.gene70470:intron 35.0%
AGAACTTCTTCTCAAAGCAT+GGG - chr3.1:66644851-66644870 MS.gene70470:CDS 35.0%
AGCAGGTAAAACCCTAATTA+CGG - chr3.1:66644373-66644392 MS.gene70470:CDS 35.0%
ATCAAGAGGACTATTCCAAA+GGG - chr3.1:66644499-66644518 MS.gene70470:intron 35.0%
ATGTCGATCCTTCATGATAA+CGG + chr3.1:66644262-66644281 None:intergenic 35.0%
CAACCAAAACCAAGGAAAAA+AGG - chr3.1:66645270-66645289 MS.gene70470:CDS 35.0%
CACAAAGTGAAACCGTAATT+AGG + chr3.1:66644388-66644407 None:intergenic 35.0%
CTAGAATCACTACCAAATGA+AGG + chr3.1:66645392-66645411 None:intergenic 35.0%
GAACATAGGTTAGTGGAAAT+TGG - chr3.1:66645069-66645088 MS.gene70470:intron 35.0%
TATACCTAGGGTTGTATCTT+TGG + chr3.1:66644112-66644131 None:intergenic 35.0%
TCATGCAGGAAAATCTTCAT+CGG - chr3.1:66644078-66644097 MS.gene70470:CDS 35.0%
TGATGGGAACATGATTGATA+TGG - chr3.1:66644978-66644997 MS.gene70470:intron 35.0%
! AACTGATTCCGTTATCATGA+AGG - chr3.1:66644251-66644270 MS.gene70470:CDS 35.0%
! GAAGTACTTTGAAAGGTATG+GGG - chr3.1:66644224-66644243 MS.gene70470:CDS 35.0%
! GTGAAGTACTTTGAAAGGTA+TGG - chr3.1:66644222-66644241 MS.gene70470:CDS 35.0%
! TTGACTCGATTCTTTGTTGT+AGG - chr3.1:66645316-66645335 MS.gene70470:intron 35.0%
!! CATATCAGGTTGAAATCAAG+AGG - chr3.1:66644485-66644504 MS.gene70470:intron 35.0%
!! CTCATTTTTCGTTTTCATGC+AGG - chr3.1:66644064-66644083 MS.gene70470:CDS 35.0%
!! CTTCATTTGGTAGTGATTCT+AGG - chr3.1:66645390-66645409 MS.gene70470:intron 35.0%
!!! CTTTTTTCCTTGGTTTTGGT+TGG + chr3.1:66645272-66645291 None:intergenic 35.0%
!!! GAACCTTTTTTCCTTGGTTT+TGG + chr3.1:66645276-66645295 None:intergenic 35.0%
AAACCTTCAGATACAGTTGC+TGG + chr3.1:66644577-66644596 None:intergenic 40.0%
ATAACCTCCCCTACTAGAAT+AGG + chr3.1:66645816-66645835 None:intergenic 40.0%
ATCATGAAGGATCGACATAC+CGG - chr3.1:66644264-66644283 MS.gene70470:CDS 40.0%
ATTCCAGCAACTGTATCTGA+AGG - chr3.1:66644571-66644590 MS.gene70470:intron 40.0%
ATTCTAGTAGGGGAGGTTAT+GGG - chr3.1:66645816-66645835 MS.gene70470:intron 40.0%
CAAGCGACCTATAAGATGTA+GGG + chr3.1:66645487-66645506 None:intergenic 40.0%
CTCAAAGCATGGGAATGTTA+TGG - chr3.1:66644861-66644880 MS.gene70470:CDS 40.0%
GATTGATATGGATGGTACTC+AGG - chr3.1:66644990-66645009 MS.gene70470:intron 40.0%
GGGAACATGATTGATATGGA+TGG - chr3.1:66644982-66645001 MS.gene70470:intron 40.0%
GTAGGTTTAGCGATTATGGT+GGG - chr3.1:66645519-66645538 MS.gene70470:CDS 40.0%
GTTATGGCCCTACATCTTAT+AGG - chr3.1:66645477-66645496 MS.gene70470:CDS 40.0%
TAACCTCCCCTACTAGAATA+GGG + chr3.1:66645815-66645834 None:intergenic 40.0%
TACTGCTGAACATAGGTTAG+TGG - chr3.1:66645062-66645081 MS.gene70470:intron 40.0%
TATTCTAGTAGGGGAGGTTA+TGG - chr3.1:66645815-66645834 MS.gene70470:intron 40.0%
TCACATCATCAATGACAAGC+AGG - chr3.1:66644356-66644375 MS.gene70470:CDS 40.0%
TCACATTGATGGAAAGCAAC+AGG + chr3.1:66645173-66645192 None:intergenic 40.0%
TCAGACACTTGCAATAACAC+TGG + chr3.1:66645208-66645227 None:intergenic 40.0%
TTAGCCAAAGATACAACCCT+AGG - chr3.1:66644105-66644124 MS.gene70470:CDS 40.0%
TTGCAAGTGTCTGACATATG+TGG - chr3.1:66645214-66645233 MS.gene70470:intron 40.0%
TTTGACGTGCAAAGATGTTG+TGG - chr3.1:66645093-66645112 MS.gene70470:intron 40.0%
! GATGATTTCAGTGGTGGTTT+TGG - chr3.1:66645554-66645573 MS.gene70470:CDS 40.0%
! GGTGGATTTGACGATTCTTA+TGG - chr3.1:66645437-66645456 MS.gene70470:intron 40.0%
! GTTGTAGGTTGAGATCAAGA+AGG - chr3.1:66645331-66645350 MS.gene70470:intron 40.0%
! TCTTATGGAAGTTTTCCTGC+TGG - chr3.1:66645452-66645471 MS.gene70470:CDS 40.0%
! TTAGTTTGAGATGCACCCTT+TGG + chr3.1:66644517-66644536 None:intergenic 40.0%
!! TTTAGCGATTATGGTGGGTA+TGG - chr3.1:66645524-66645543 MS.gene70470:CDS 40.0%
AAGTGTCTGACATATGTGGC+GGG - chr3.1:66645218-66645237 MS.gene70470:intron 45.0%
ACATCTTATAGGTCGCTTGG+AGG - chr3.1:66645488-66645507 MS.gene70470:CDS 45.0%
ACTGGTGGAGGTTATGAATC+TGG - chr3.1:66645758-66645777 MS.gene70470:intron 45.0%
AGAGGAGGAGAATCCTCATT+CGG - chr3.1:66645620-66645639 MS.gene70470:intron 45.0%
AGGAGGACCCTATTCTAGTA+GGG - chr3.1:66645805-66645824 MS.gene70470:intron 45.0%
CAAGTGTCTGACATATGTGG+CGG - chr3.1:66645217-66645236 MS.gene70470:intron 45.0%
CCAAGCGACCTATAAGATGT+AGG + chr3.1:66645488-66645507 None:intergenic 45.0%
CCTACATCTTATAGGTCGCT+TGG - chr3.1:66645485-66645504 MS.gene70470:CDS 45.0%
GAATCCTCATTCGGTTACTC+TGG - chr3.1:66645629-66645648 MS.gene70470:intron 45.0%
GGTAGGTTTAGCGATTATGG+TGG - chr3.1:66645518-66645537 MS.gene70470:CDS 45.0%
GGTGGTAGGTTTAGCGATTA+TGG - chr3.1:66645515-66645534 MS.gene70470:CDS 45.0%
GTGATAAACCCAAATCCTCG+AGG + chr3.1:66644294-66644313 None:intergenic 45.0%
GTGATGCTACTGCTGAACAT+AGG - chr3.1:66645055-66645074 MS.gene70470:intron 45.0%
TATCTGCCCAACCAAAACCA+AGG - chr3.1:66645262-66645281 MS.gene70470:CDS 45.0%
TGCAGGAAAATCTTCATCGG+AGG - chr3.1:66644081-66644100 MS.gene70470:CDS 45.0%
TTCTAGTAGGGGAGGTTATG+GGG - chr3.1:66645817-66645836 MS.gene70470:intron 45.0%
! AGTTTTCCTGCTGGAGGTTA+TGG - chr3.1:66645461-66645480 MS.gene70470:CDS 45.0%
! CGTTCTTACAATGGCGGTTT+TGG - chr3.1:66645416-66645435 MS.gene70470:intron 45.0%
! GGATGGTACTCAGGTTAGTT+TGG - chr3.1:66644999-66645018 MS.gene70470:intron 45.0%
! TATAGGTCGCTTGGAGGTTT+TGG - chr3.1:66645494-66645513 MS.gene70470:CDS 45.0%
! TATGGAAGTTTTCCTGCTGG+AGG - chr3.1:66645455-66645474 MS.gene70470:CDS 45.0%
! TCTAGGGCACGTTCTTACAA+TGG - chr3.1:66645407-66645426 MS.gene70470:intron 45.0%
!! TCTTACAATGGCGGTTTTGG+TGG - chr3.1:66645419-66645438 MS.gene70470:intron 45.0%
!!! AGTGGTGGTTTTGGAGGTTA+TGG - chr3.1:66645563-66645582 MS.gene70470:CDS 45.0%
!!! GATTTCAGTGGTGGTTTTGG+AGG - chr3.1:66645557-66645576 MS.gene70470:CDS 45.0%
AAATCCACGGGAACGCTTAG+TGG + chr3.1:66644911-66644930 None:intergenic 50.0%
AAGAGAGAAGAGAGCGAACC+CGG + chr3.1:66643997-66644016 None:intergenic 50.0%
AAGTCCGATAACCCTCACTC+CGG - chr3.1:66643957-66643976 MS.gene70470:CDS 50.0%
ACTAAGCGTTCCCGTGGATT+TGG - chr3.1:66644910-66644929 MS.gene70470:CDS 50.0%
AGGGCACGTTCTTACAATGG+CGG - chr3.1:66645410-66645429 MS.gene70470:intron 50.0%
CAGGAGGACCCTATTCTAGT+AGG - chr3.1:66645804-66645823 MS.gene70470:intron 50.0%
GGAGGACCCTATTCTAGTAG+GGG - chr3.1:66645806-66645825 MS.gene70470:intron 50.0%
GGAGGTGGAGATGATTTCAG+TGG - chr3.1:66645545-66645564 MS.gene70470:CDS 50.0%
GGTGGAGATGATTTCAGTGG+TGG - chr3.1:66645548-66645567 MS.gene70470:CDS 50.0%
GTGAGGGTTATCGGACTTTC+TGG + chr3.1:66643955-66643974 None:intergenic 50.0%
TCTAGTAGGGGAGGTTATGG+GGG - chr3.1:66645818-66645837 MS.gene70470:intron 50.0%
TTTGGAGGTTATGGAGGCAG+TGG - chr3.1:66645572-66645591 MS.gene70470:CDS 50.0%
!! AGCGATTATGGTGGGTATGG+AGG - chr3.1:66645527-66645546 MS.gene70470:CDS 50.0%
!! GATTATGGTGGGTATGGAGG+TGG - chr3.1:66645530-66645549 MS.gene70470:CDS 50.0%
!!! GGTGGTTTTGGAGGTTATGG+AGG - chr3.1:66645566-66645585 MS.gene70470:CDS 50.0%
AGAGAGAAGAGAGCGAACCC+GGG + chr3.1:66643996-66644015 None:intergenic 55.0%
CACCACCATATCCACCACCA+AGG + chr3.1:66645679-66645698 None:intergenic 55.0%
CACGGGAACGCTTAGTGGTA+TGG + chr3.1:66644906-66644925 None:intergenic 55.0%
CATACCACTAAGCGTTCCCG+TGG - chr3.1:66644904-66644923 MS.gene70470:CDS 55.0%
CCCCCATGTAGGATCCATAG+CGG + chr3.1:66645655-66645674 None:intergenic 55.0%
CCGCTATGGATCCTACATGG+GGG - chr3.1:66645652-66645671 MS.gene70470:intron 55.0%
CGCTATGGATCCTACATGGG+GGG - chr3.1:66645653-66645672 MS.gene70470:intron 55.0%
GCCGCTATGGATCCTACATG+GGG - chr3.1:66645651-66645670 MS.gene70470:intron 55.0%
GGACCCTATTCTAGTAGGGG+AGG - chr3.1:66645809-66645828 MS.gene70470:intron 55.0%
GGAGGTTATGAATCTGGCCC+TGG - chr3.1:66645764-66645783 MS.gene70470:intron 55.0%
GGCCGCTATGGATCCTACAT+GGG - chr3.1:66645650-66645669 MS.gene70470:intron 55.0%
TATGAATCTGGCCCTGGTGC+TGG - chr3.1:66645770-66645789 MS.gene70470:intron 55.0%
TATGGCGGTTATGGTGGTCC+AGG - chr3.1:66645734-66645753 MS.gene70470:intron 55.0%
TCATAACCTCCACCAGTGCC+TGG + chr3.1:66645755-66645774 None:intergenic 55.0%
TCCCACCAAAACCAGCACCA+GGG + chr3.1:66645784-66645803 None:intergenic 55.0%
TGGCCGCTATGGATCCTACA+TGG - chr3.1:66645649-66645668 MS.gene70470:intron 55.0%
TTAGGCCCATATGGGAGAGG+TGG - chr3.1:66645704-66645723 MS.gene70470:CDS 55.0%
TTCGGTTACTCTGGCCGCTA+TGG - chr3.1:66645638-66645657 MS.gene70470:intron 55.0%
! GGTGGTTATGCGGGCTATAG+AGG - chr3.1:66645602-66645621 MS.gene70470:intron 55.0%
! GGTTATGCGGGCTATAGAGG+AGG - chr3.1:66645605-66645624 MS.gene70470:intron 55.0%
!! AGGTCGCTTGGAGGTTTTGG+TGG - chr3.1:66645497-66645516 MS.gene70470:CDS 55.0%
!! CGCTTGGAGGTTTTGGTGGT+AGG - chr3.1:66645501-66645520 MS.gene70470:CDS 55.0%
!! CTTGGTGGTGGATATGGTGG+TGG - chr3.1:66645677-66645696 MS.gene70470:intron 55.0%
!! GGATATGGTGGTGGTGGGTT+AGG - chr3.1:66645686-66645705 MS.gene70470:intron 55.0%
!! GTGGTGGATATGGTGGTGGT+GGG - chr3.1:66645681-66645700 MS.gene70470:intron 55.0%
!! GTGGTGGGTTAGGCCCATAT+GGG - chr3.1:66645696-66645715 MS.gene70470:CDS 55.0%
!! TGGTGGTGCTGGTGGTTATG+CGG - chr3.1:66645592-66645611 MS.gene70470:intron 55.0%
ACCGGTCGTCCTCGAGGATT+TGG - chr3.1:66644282-66644301 MS.gene70470:CDS 60.0%
CCCAAATCCTCGAGGACGAC+CGG + chr3.1:66644286-66644305 None:intergenic 60.0%
CCCATATGGGAGAGGTGGTG+GGG - chr3.1:66645709-66645728 MS.gene70470:CDS 60.0%
CCCCACCACCTCTCCCATAT+GGG + chr3.1:66645712-66645731 None:intergenic 60.0%
CCGGTCGTCCTCGAGGATTT+GGG - chr3.1:66644283-66644302 MS.gene70470:CDS 60.0%
GATAACCCTCACTCCGGCGA+CGG - chr3.1:66643963-66643982 MS.gene70470:CDS 60.0%
GCCCATATGGGAGAGGTGGT+GGG - chr3.1:66645708-66645727 MS.gene70470:CDS 60.0%
GCGGCCAGAGTAACCGAATG+AGG + chr3.1:66645636-66645655 None:intergenic 60.0%
GGAGGTTATGGAGGCAGTGG+TGG - chr3.1:66645575-66645594 MS.gene70470:CDS 60.0%
GGGTTAGGCCCATATGGGAG+AGG - chr3.1:66645701-66645720 MS.gene70470:CDS 60.0%
GGTTATGGTGGTCCAGGCAC+TGG - chr3.1:66645740-66645759 MS.gene70470:intron 60.0%
GTAGGGCCATAACCTCCAGC+AGG + chr3.1:66645470-66645489 None:intergenic 60.0%
GTCCCACCAAAACCAGCACC+AGG + chr3.1:66645785-66645804 None:intergenic 60.0%
GTCGCCGGAGTGAGGGTTAT+CGG + chr3.1:66643964-66643983 None:intergenic 60.0%
! CCATATGGGAGAGGTGGTGG+GGG - chr3.1:66645710-66645729 MS.gene70470:CDS 60.0%
! TATGGAGGCAGTGGTGGTGC+TGG - chr3.1:66645581-66645600 MS.gene70470:CDS 60.0%
!! GGCCTTGGTGGTGGATATGG+TGG - chr3.1:66645674-66645693 MS.gene70470:intron 60.0%
!! GGTGGTGCTGGTGGTTATGC+GGG - chr3.1:66645593-66645612 MS.gene70470:intron 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG - chr3.1:66645680-66645699 MS.gene70470:intron 60.0%
!! GGTGGTGGGTTAGGCCCATA+TGG - chr3.1:66645695-66645714 MS.gene70470:CDS 60.0%
!! TATGGTGGTCCAGGCACTGG+TGG - chr3.1:66645743-66645762 MS.gene70470:intron 60.0%
!!! GCCCTGGTGCTGGTTTTGGT+GGG - chr3.1:66645780-66645799 MS.gene70470:intron 60.0%
!!! GCTGGTTTTGGTGGGACAGG+AGG - chr3.1:66645788-66645807 MS.gene70470:intron 60.0%
!!! GGTGCTGGTTTTGGTGGGAC+AGG - chr3.1:66645785-66645804 MS.gene70470:intron 60.0%
!!! TCTGGCCCTGGTGCTGGTTT+TGG - chr3.1:66645776-66645795 MS.gene70470:intron 60.0%
ACCACCAAGGCCCCCCATGT+AGG + chr3.1:66645666-66645685 None:intergenic 65.0%
CCCCCACCACCTCTCCCATA+TGG + chr3.1:66645713-66645732 None:intergenic 65.0%
CGACATACCGGTCGTCCTCG+AGG - chr3.1:66644276-66644295 MS.gene70470:CDS 65.0%
GAAGAGAGCGAACCCGGGAC+TGG + chr3.1:66643991-66644010 None:intergenic 65.0%
GGATCCTACATGGGGGGCCT+TGG - chr3.1:66645659-66645678 MS.gene70470:intron 65.0%
GGCCCATATGGGAGAGGTGG+TGG - chr3.1:66645707-66645726 MS.gene70470:CDS 65.0%
GGGGGCTATGGCGGTTATGG+TGG - chr3.1:66645728-66645747 MS.gene70470:intron 65.0%
GGTGGGGGCTATGGCGGTTA+TGG - chr3.1:66645725-66645744 MS.gene70470:intron 65.0%
TACATGGGGGGCCTTGGTGG+TGG - chr3.1:66645665-66645684 MS.gene70470:intron 65.0%
TCCTACATGGGGGGCCTTGG+TGG - chr3.1:66645662-66645681 MS.gene70470:intron 65.0%
!! AGAGGTGGTGGGGGCTATGG+CGG - chr3.1:66645719-66645738 MS.gene70470:intron 65.0%
!! GGGGGCCTTGGTGGTGGATA+TGG - chr3.1:66645671-66645690 MS.gene70470:intron 65.0%
!!! GGCCCTGGTGCTGGTTTTGG+TGG - chr3.1:66645779-66645798 MS.gene70470:intron 65.0%
! GGAGGCAGTGGTGGTGCTGG+TGG - chr3.1:66645584-66645603 MS.gene70470:intron 70.0%
!! GGGAGAGGTGGTGGGGGCTA+TGG - chr3.1:66645716-66645735 MS.gene70470:intron 70.0%
!! GGTGGTCCAGGCACTGGTGG+AGG - chr3.1:66645746-66645765 MS.gene70470:intron 70.0%
TGGCGCCGTCGCCGGAGTGA+GGG + chr3.1:66643971-66643990 None:intergenic 75.0%
! CTGGCGCCGTCGCCGGAGTG+AGG + chr3.1:66643972-66643991 None:intergenic 80.0%
! TCCGGCGACGGCGCCAGTCC+CGG - chr3.1:66643975-66643994 MS.gene70470:CDS 80.0%
! CCGGCGACGGCGCCAGTCCC+GGG - chr3.1:66643976-66643995 MS.gene70470:CDS 85.0%
!! CCCGGGACTGGCGCCGTCGC+CGG + chr3.1:66643979-66643998 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 66643945 66645871 66643945 ID=MS.gene70470
chr3.1 mRNA 66643945 66645871 66643945 ID=MS.gene70470.t1;Parent=MS.gene70470
chr3.1 exon 66645819 66645871 66645819 ID=MS.gene70470.t1.exon1;Parent=MS.gene70470.t1
chr3.1 CDS 66645819 66645871 66645819 ID=cds.MS.gene70470.t1;Parent=MS.gene70470.t1
chr3.1 exon 66645690 66645730 66645690 ID=MS.gene70470.t1.exon2;Parent=MS.gene70470.t1
chr3.1 CDS 66645690 66645730 66645690 ID=cds.MS.gene70470.t1;Parent=MS.gene70470.t1
chr3.1 exon 66645439 66645602 66645439 ID=MS.gene70470.t1.exon3;Parent=MS.gene70470.t1
chr3.1 CDS 66645439 66645602 66645439 ID=cds.MS.gene70470.t1;Parent=MS.gene70470.t1
chr3.1 exon 66645224 66645323 66645224 ID=MS.gene70470.t1.exon4;Parent=MS.gene70470.t1
chr3.1 CDS 66645224 66645323 66645224 ID=cds.MS.gene70470.t1;Parent=MS.gene70470.t1
chr3.1 exon 66644805 66644974 66644805 ID=MS.gene70470.t1.exon5;Parent=MS.gene70470.t1
chr3.1 CDS 66644805 66644974 66644805 ID=cds.MS.gene70470.t1;Parent=MS.gene70470.t1
chr3.1 exon 66643945 66644478 66643945 ID=MS.gene70470.t1.exon6;Parent=MS.gene70470.t1
chr3.1 CDS 66643945 66644478 66643945 ID=cds.MS.gene70470.t1;Parent=MS.gene70470.t1
Gene Sequence

>MS.gene70470

ATGGCTTCCAGAAAGTCCGATAACCCTCACTCCGGCGACGGCGCCAGTCCCGGGTTCGCTCTCTTCTCTCTTCTTACACATTCAATTCGCTTCGTCCCCGATTTCTCTTTTTCACTATTCTCATTTTTCGTTTTCATGCAGGAAAATCTTCATCGGAGGTTTAGCCAAAGATACAACCCTAGGTATAAACATCTTTTCATTTTTTTGATATTTTTCTTTTAATTTTCAGTATTATTTATTTTATAGGTTTTGATATTTTGATTCTGCAGAGACTTTTGTGAAGTACTTTGAAAGGTATGGGGAGATAACTGATTCCGTTATCATGAAGGATCGACATACCGGTCGTCCTCGAGGATTTGGGTTTATCACTTATGCGGATGCTTCTGTTGTTGATCAAGTTATTCAGGAGAATCACATCATCAATGACAAGCAGGTAAAACCCTAATTACGGTTTCACTTTGTGTTTTGTAGATTCAGTTGAATTTTTGATTTGGGCTGTTTTCAAATGTTTACTTTGTTTTCATTGTTGATTTGTCTGTTCATATCAGGTTGAAATCAAGAGGACTATTCCAAAGGGTGCATCTCAAACTAATGACTTCAAAACTAAGAAGATTTTTGTTGGTGGCATTCCAGCAACTGTATCTGAAGGTTTGTCTTCAGCACTTATAATTTTTTTGATATAATCAATGTTCGTTGTGTGCTTTGATTTGTTTATATAACAATTAGTGTTTATTGTGTGTTTATACAAGTTTGTTAGTCTGTATTGTTTGTTTTCGAGTGAAAGTATGGTATTGTGTGTTAGTGAGCTTGTTAGTTTGTATTGTTTGTTTTCGAGTGAAAGTATGGTATTGTGTGTTATTGAGCTTATTGTATGCTTATCTTGGGTGTGTGTGTCAGATGAGCTCAAGAACTTCTTCTCAAAGCATGGGAATGTTATGGAGCACGAGATCATACGCGACCATACCACTAAGCGTTCCCGTGGATTTGGTTTTGTAGTTTTTGATAGCGATAAGGCCGTTGATAATTTGTTAGCTGATGGGAACATGATTGATATGGATGGTACTCAGGTTAGTTTGGTTTGTTTATTTAGGGTGTTTAAAATTGAAACTGGTGATGCTACTGCTGAACATAGGTTAGTGGAAATTGGTTTTGACGTGCAAAGATGTTGTGGAATAATTGATAATAATAATAATAGTAGTAGTAGTAATAATAATAATTGCGGATTCCTGTTGCTTTCCATCAATGTGATGTCTCACTTTGCCAGTGTTATTGCAAGTGTCTGACATATGTGGCGGGCTAACTGCACATTTTATCATTTATCTGCCCAACCAAAACCAAGGAAAAAAGGTTCTATTTATGAATGAATCTAGGTTGACTCGATTCTTTGTTGTAGGTTGAGATCAAGAAGGCTGAACCAAAGAAATCTTCAAATTCATCTTCTTTTCCTTCATTTGGTAGTGATTCTAGGGCACGTTCTTACAATGGCGGTTTTGGTGGATTTGACGATTCTTATGGAAGTTTTCCTGCTGGAGGTTATGGCCCTACATCTTATAGGTCGCTTGGAGGTTTTGGTGGTAGGTTTAGCGATTATGGTGGGTATGGAGGTGGAGATGATTTCAGTGGTGGTTTTGGAGGTTATGGAGGCAGTGGTGGTGCTGGTGGTTATGCGGGCTATAGAGGAGGAGAATCCTCATTCGGTTACTCTGGCCGCTATGGATCCTACATGGGGGGCCTTGGTGGTGGATATGGTGGTGGTGGGTTAGGCCCATATGGGAGAGGTGGTGGGGGCTATGGCGGTTATGGTGGTCCAGGCACTGGTGGAGGTTATGAATCTGGCCCTGGTGCTGGTTTTGGTGGGACAGGAGGACCCTATTCTAGTAGGGGAGGTTATGGGGGCAGTAGTCGTTACCATCCTTACACAAGATAG
Protein sequence

>MS.gene70470.t1

MASRKSDNPHSGDGASPGKIFIGGLAKDTTLETFVKYFERYGEITDSVIMKDRHTGRPRGFGFITYADASVVDQVIQENHIINDKQVEIKRTIPKGASQTNDFKTKKIFVGGIPATVSEDELKNFFSKHGNVMEHEIIRDHTTKRSRGFGFVVFDSDKAVDNLLADGNMIDMDGTQVEIKKAEPKKSSNSSSFPSFGSDSRARSYNGGFGGFDDSYGSFPAGGYGPTSYRSLGGFGGRFSDYGGYGGGDDFSGGFGGYGGSGGAGGYAGYRGGESSFGYSGRYGSYMGGLGGGYGGGGLGPYGRGGGGYGGYGGPGTGGGYESGPGAGFGGTGGPYSSRGGYGGSSRYHPYTR