Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41414.t1 | RHN81845.1 | 95 | 222 | 8 | 2 | 1 | 221 | 35 | 254 | 1.70E-110 | 408.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41414.t1 | Q9M2Z4 | 73.6 | 182 | 42 | 3 | 5 | 185 | 4 | 180 | 3.5e-67 | 256.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41414.t1 | A0A072VQL5 | 95.0 | 222 | 8 | 2 | 1 | 221 | 1 | 220 | 1.3e-110 | 408.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050670 | MS.gene41414 | -0.802916 | 4.47E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene41414 | -0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene41414 | -0.800897 | 1.16E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41414.t1 | MTR_1g100727 | 95.045 | 222 | 8 | 2 | 1 | 221 | 1 | 220 | 3.62e-152 | 421 |
| MS.gene41414.t1 | MTR_6g054890 | 67.234 | 235 | 59 | 5 | 3 | 221 | 2 | 234 | 2.79e-103 | 298 |
| MS.gene41414.t1 | MTR_6g027160 | 49.068 | 161 | 70 | 3 | 19 | 171 | 17 | 173 | 9.62e-47 | 153 |
| MS.gene41414.t1 | MTR_3g105860 | 54.639 | 97 | 44 | 0 | 75 | 171 | 2 | 98 | 3.53e-33 | 115 |
| MS.gene41414.t1 | MTR_3g081290 | 38.542 | 96 | 59 | 0 | 76 | 171 | 35 | 130 | 6.41e-17 | 77.0 |
| MS.gene41414.t1 | MTR_6g027060 | 53.333 | 60 | 28 | 0 | 110 | 169 | 27 | 86 | 1.29e-16 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41414.t1 | AT3G48890 | 66.507 | 209 | 63 | 4 | 5 | 212 | 4 | 206 | 2.49e-90 | 265 |
| MS.gene41414.t1 | AT5G52240 | 61.991 | 221 | 79 | 3 | 3 | 221 | 2 | 219 | 1.56e-88 | 260 |
| MS.gene41414.t1 | AT5G52240 | 72.674 | 172 | 43 | 2 | 3 | 172 | 2 | 171 | 2.01e-86 | 253 |
| MS.gene41414.t1 | AT2G24940 | 50.515 | 97 | 48 | 0 | 75 | 171 | 2 | 98 | 1.46e-29 | 106 |
| MS.gene41414.t1 | AT4G14965 | 30.921 | 152 | 82 | 2 | 20 | 171 | 9 | 137 | 8.19e-17 | 77.0 |
| MS.gene41414.t1 | AT4G14965 | 39.759 | 83 | 50 | 0 | 76 | 158 | 42 | 124 | 1.63e-15 | 72.4 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCGTTTACTACGTCCTTTC+TGG | 0.101868 | 1.4:+78548982 | MS.gene41414:CDS |
| ACCCTTGCTTATGGCTATTA+AGG | 0.124798 | 1.4:+78549155 | MS.gene41414:CDS |
| CTCTTGGAAGTTGCGGGATT+GGG | 0.149394 | 1.4:-78549037 | None:intergenic |
| GATTTGACCGGTGATATTTC+TGG | 0.252823 | 1.4:+78558219 | MS.gene41414:CDS |
| AGGCGATGATGGACTCCTTT+AGG | 0.261253 | 1.4:-78548909 | None:intergenic |
| ACCGGTGATATTTCTGGTCT+TGG | 0.268409 | 1.4:+78558225 | MS.gene41414:CDS |
| GGCGATGATGGACTCCTTTA+GGG | 0.276975 | 1.4:-78548908 | None:intergenic |
| TACGTCCTTTCTGGTCTCTT+TGG | 0.279840 | 1.4:+78548991 | MS.gene41414:CDS |
| ATTTGAGCTTGACGCGTTAC+AGG | 0.301755 | 1.4:+78558251 | MS.gene41414:CDS |
| CCTCTTGGAAGTTGCGGGAT+TGG | 0.303689 | 1.4:-78549038 | None:intergenic |
| GGACTGGGAATACAAGTTTA+TGG | 0.304869 | 1.4:+78558272 | MS.gene41414:CDS |
| CTCCTCCTCTTGGAAGTTGC+GGG | 0.307440 | 1.4:-78549043 | None:intergenic |
| GTGGATTCAGCTTCAGTAAC+TGG | 0.323655 | 1.4:-78558327 | None:intergenic |
| CCCTTGCTTATGGCTATTAA+GGG | 0.325886 | 1.4:+78549156 | MS.gene41414:CDS |
| GGACCGTATGCTTTATTTGC+TGG | 0.331470 | 1.4:+78558150 | MS.gene41414:CDS |
| TCATGATAAACCCTTGCTTA+TGG | 0.362127 | 1.4:+78549146 | MS.gene41414:CDS |
| TTATCTGCCTCAACATTTGA+AGG | 0.394915 | 1.4:-78558444 | None:intergenic |
| AGCTTGACGCGTTACAGGAC+TGG | 0.403993 | 1.4:+78558256 | MS.gene41414:CDS |
| GTGATTTCACCTAGCTGAAC+AGG | 0.406413 | 1.4:-78549090 | None:intergenic |
| GACCACTTTCTTCACCATCT+TGG | 0.410349 | 1.4:+78548948 | MS.gene41414:CDS |
| TCTTGGAAGTTGCGGGATTG+GGG | 0.417269 | 1.4:-78549036 | None:intergenic |
| TGTAACGCGTCAAGCTCAAA+TGG | 0.428078 | 1.4:-78558249 | None:intergenic |
| GAATCCACGGGTGAAGCATC+AGG | 0.447705 | 1.4:+78558342 | MS.gene41414:CDS |
| CGATGGCCGGCGTTCAGCTG+TGG | 0.449798 | 1.4:+78548881 | None:intergenic |
| TAGACCGGTGTAGGCGATGA+TGG | 0.470054 | 1.4:-78548920 | None:intergenic |
| ATAAGCCTTGAGCTCTTCTT+CGG | 0.471949 | 1.4:-78549112 | None:intergenic |
| GTTTATGGAGAAGTATGTTA+AGG | 0.473963 | 1.4:+78558287 | MS.gene41414:CDS |
| ACCAGAAAGGACGTAGTAAA+CGG | 0.488478 | 1.4:-78548983 | None:intergenic |
| CCTCCTCCTCTTGGAAGTTG+CGG | 0.490295 | 1.4:-78549044 | None:intergenic |
| GTATGCTTTATTTGCTGGCA+AGG | 0.504997 | 1.4:+78558155 | MS.gene41414:CDS |
| TATCTGCCTCAACATTTGAA+GGG | 0.520292 | 1.4:-78558443 | None:intergenic |
| GGCAAATAGAAGAGCCAAGA+TGG | 0.520312 | 1.4:-78548962 | None:intergenic |
| GCTTGACGCGTTACAGGACT+GGG | 0.526300 | 1.4:+78558257 | MS.gene41414:CDS |
| GAAAGTGGTCGGAGATAGAC+CGG | 0.532113 | 1.4:-78548935 | None:intergenic |
| TCTATGATGTCTCTCAGAGC+AGG | 0.537470 | 1.4:+78549184 | MS.gene41414:CDS |
| TTTGATCCGCCATCTTCAGT+TGG | 0.541409 | 1.4:-78558393 | None:intergenic |
| GATGGCCGGCGTTCAGCTGT+GGG | 0.545916 | 1.4:+78548882 | None:intergenic |
| GAAGAGCTCAAGGCTTATGA+TGG | 0.549967 | 1.4:+78549117 | MS.gene41414:CDS |
| TTTGATGAGAAAGATTTGAC+CGG | 0.550185 | 1.4:+78558207 | MS.gene41414:CDS |
| CAACTTCCAAGAGGAGGAGG+AGG | 0.556609 | 1.4:+78549047 | MS.gene41414:CDS |
| ATCTGCCTCAACATTTGAAG+GGG | 0.560772 | 1.4:-78558442 | None:intergenic |
| ACACAACAACGAGCGATGGC+CGG | 0.561098 | 1.4:+78548868 | None:intergenic |
| GCTGTTAATCCAACTGAAGA+TGG | 0.564157 | 1.4:+78558384 | MS.gene41414:CDS |
| TCAGCTGTGGGAAACCCTAA+AGG | 0.566475 | 1.4:+78548894 | MS.gene41414:CDS |
| GTAACGCGTCAAGCTCAAAT+GGG | 0.568003 | 1.4:-78558248 | None:intergenic |
| AGTAGCCAAAGAGACCAGAA+AGG | 0.568870 | 1.4:-78548996 | None:intergenic |
| CCGCAACTTCCAAGAGGAGG+AGG | 0.571017 | 1.4:+78549044 | MS.gene41414:CDS |
| CTTGGAAGTTGCGGGATTGG+GGG | 0.575348 | 1.4:-78549035 | None:intergenic |
| TTGCCAGCAAATAAAGCATA+CGG | 0.582093 | 1.4:-78558153 | None:intergenic |
| ATGGAGAAGTATGTTAAGGT+CGG | 0.585025 | 1.4:+78558291 | MS.gene41414:CDS |
| GGTCGGAGATAGACCGGTGT+AGG | 0.586223 | 1.4:-78548929 | None:intergenic |
| ACCCTTAATAGCCATAAGCA+AGG | 0.587630 | 1.4:-78549157 | None:intergenic |
| AATAAAGCATACGGTCCACC+CGG | 0.588408 | 1.4:-78558144 | None:intergenic |
| GTTACTGAAGCTGAATCCAC+GGG | 0.589713 | 1.4:+78558330 | MS.gene41414:CDS |
| CCCTTAATAGCCATAAGCAA+GGG | 0.597837 | 1.4:-78549156 | None:intergenic |
| GGTTTCCCACAGCTGAACGC+CGG | 0.599948 | 1.4:-78548887 | None:intergenic |
| AGTTACTGAAGCTGAATCCA+CGG | 0.600398 | 1.4:+78558329 | MS.gene41414:CDS |
| GAGTCCATCATCGCCTACAC+CGG | 0.603823 | 1.4:+78548916 | MS.gene41414:CDS |
| TGAACAGGTGGTCGGAGAGG+TGG | 0.609555 | 1.4:-78549075 | None:intergenic |
| CACCTAGCTGAACAGGTGGT+CGG | 0.610497 | 1.4:-78549083 | None:intergenic |
| CTCCGACCACCTGTTCAGCT+AGG | 0.610888 | 1.4:+78549081 | MS.gene41414:CDS |
| AGCTGAACAGGTGGTCGGAG+AGG | 0.612379 | 1.4:-78549078 | None:intergenic |
| ACCTGCAGCTGTTAAAAGTG+AGG | 0.614328 | 1.4:+78558416 | MS.gene41414:CDS |
| ATCCCGCAACTTCCAAGAGG+AGG | 0.616485 | 1.4:+78549041 | MS.gene41414:CDS |
| AGCCAAGATGGTGAAGAAAG+TGG | 0.619359 | 1.4:-78548950 | None:intergenic |
| GGAAACCCCTTCAAATGTTG+AGG | 0.619610 | 1.4:+78558437 | MS.gene41414:CDS |
| AATCACCGAAGAAGAGCTCA+AGG | 0.634309 | 1.4:+78549107 | MS.gene41414:CDS |
| AAGATGGTGAAGAAAGTGGT+CGG | 0.644524 | 1.4:-78548946 | None:intergenic |
| AGATCCTGATGCTTCACCCG+TGG | 0.646182 | 1.4:-78558346 | None:intergenic |
| GCCAAGACCAGAAATATCAC+CGG | 0.647612 | 1.4:-78558226 | None:intergenic |
| GTTAATCCAACTGAAGATGG+CGG | 0.680061 | 1.4:+78558387 | MS.gene41414:CDS |
| ATTTCACCTAGCTGAACAGG+TGG | 0.686884 | 1.4:-78549087 | None:intergenic |
| CCAATCCCGCAACTTCCAAG+AGG | 0.696455 | 1.4:+78549038 | MS.gene41414:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 78548883 | 78558473 | 78548883 | ID=MS.gene41414 |
| chr1.4 | mRNA | 78548883 | 78558473 | 78548883 | ID=MS.gene41414.t1;Parent=MS.gene41414 |
| chr1.4 | exon | 78548883 | 78549205 | 78548883 | ID=MS.gene41414.t1.exon1;Parent=MS.gene41414.t1 |
| chr1.4 | CDS | 78548883 | 78549205 | 78548883 | ID=cds.MS.gene41414.t1;Parent=MS.gene41414.t1 |
| chr1.4 | exon | 78558131 | 78558473 | 78558131 | ID=MS.gene41414.t1.exon2;Parent=MS.gene41414.t1 |
| chr1.4 | CDS | 78558131 | 78558473 | 78558131 | ID=cds.MS.gene41414.t1;Parent=MS.gene41414.t1 |
| Gene Sequence |
| Protein sequence |
