Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41438.t1 | XP_024634934.1 | 94.7 | 150 | 8 | 0 | 1 | 150 | 66 | 215 | 3.40E-73 | 284.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41438.t1 | A0A396J2C2 | 94.7 | 150 | 8 | 0 | 1 | 150 | 66 | 215 | 2.4e-73 | 284.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050122 | MS.gene41438 | 0.827027 | 1.96E-54 | -1.69E-46 |
| MS.gene051360 | MS.gene41438 | 0.833159 | 6.26E-56 | -1.69E-46 |
| MS.gene053318 | MS.gene41438 | 0.809558 | 1.78E-50 | -1.69E-46 |
| MS.gene055666 | MS.gene41438 | 0.812393 | 4.34E-51 | -1.69E-46 |
| MS.gene05601 | MS.gene41438 | 0.838615 | 2.59E-57 | -1.69E-46 |
| MS.gene057289 | MS.gene41438 | 0.801128 | 1.04E-48 | -1.69E-46 |
| MS.gene059711 | MS.gene41438 | 0.819457 | 1.15E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene41438 | 0.828647 | 8.01E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41438.t1 | MTR_3g110115 | 57.764 | 161 | 48 | 4 | 1 | 150 | 1 | 152 | 1.04e-47 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41438.t1 | AT5G25770 | 52.703 | 148 | 63 | 3 | 7 | 150 | 5 | 149 | 6.05e-47 | 155 |
| MS.gene41438.t1 | AT5G25770 | 52.703 | 148 | 63 | 3 | 7 | 150 | 63 | 207 | 1.91e-46 | 156 |
| MS.gene41438.t1 | AT5G25770 | 52.703 | 148 | 63 | 3 | 7 | 150 | 66 | 210 | 2.05e-46 | 156 |
Find 0 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTAATTAATTTGATTTCAT+AGG | - | 21206:412-431 | MS.gene41438:CDS | 15.0% |
| !!! | CTAATTAATTTGATTTCATA+GGG | - | 21206:413-432 | MS.gene41438:CDS | 15.0% |
| !!! | TTGATTAGCTTATAATAAAA+AGG | - | 21206:675-694 | MS.gene41438:intron | 15.0% |
| ! | AAGTTCTCAATATCTTTCTT+TGG | + | 21206:326-345 | MS.gene41438:intergenic | 25.0% |
| !! | GAATTTCATTTCTAGAAGAA+TGG | + | 21206:199-218 | MS.gene41438:intergenic | 25.0% |
| !!! | GAAATATTCGTTGATTTTGT+AGG | - | 21206:447-466 | MS.gene41438:intron | 25.0% |
| CATGATAAATCCTATTTCCT+AGG | + | 21206:654-673 | MS.gene41438:intergenic | 30.0% | |
| GAATCTCTACTTAACAATAG+AGG | - | 21206:367-386 | MS.gene41438:CDS | 30.0% | |
| TATACTTGTTTGTACTGTGA+AGG | + | 21206:555-574 | MS.gene41438:intergenic | 30.0% | |
| TGATTTCATAATCTCCTCAT+CGG | + | 21206:300-319 | MS.gene41438:intergenic | 30.0% | |
| !! | GAACTTCAATGATTTGCTAA+AGG | - | 21206:340-359 | MS.gene41438:CDS | 30.0% |
| !!! | ATTCGTTGATTTTGTAGGAA+GGG | - | 21206:452-471 | MS.gene41438:intron | 30.0% |
| !!! | CATCGATTTTTTTTTGCCTT+TGG | - | 21206:717-736 | MS.gene41438:intron | 30.0% |
| !!! | TATTCGTTGATTTTGTAGGA+AGG | - | 21206:451-470 | MS.gene41438:intron | 30.0% |
| GGATTTGAAGAAACTCTGAT+CGG | + | 21206:108-127 | MS.gene41438:intergenic | 35.0% | |
| TTCAAGCTCAAAATCAACAC+AGG | + | 21206:493-512 | MS.gene41438:intergenic | 35.0% | |
| TTGTTCTACTCCTAGGAAAT+AGG | - | 21206:641-660 | MS.gene41438:CDS | 35.0% | |
| ! | ACTCATTTTGTTCTACTCCT+AGG | - | 21206:634-653 | MS.gene41438:CDS | 35.0% |
| ! | GAATTTGCTCTGTTCTTCAA+TGG | + | 21206:86-105 | MS.gene41438:intergenic | 35.0% |
| !! | CTTCAATGATTTGCTAAAGG+AGG | - | 21206:343-362 | MS.gene41438:CDS | 35.0% |
| !!! | TGTGTTGATTTTGAGCTTGA+AGG | - | 21206:492-511 | MS.gene41438:intron | 35.0% |
| !!! | TTCGTTGATTTTGTAGGAAG+GGG | - | 21206:453-472 | MS.gene41438:intron | 35.0% |
| AGGTCTTCTTGTCTGCTTAT+CGG | + | 21206:521-540 | MS.gene41437:intergenic | 40.0% | |
| TAGTAAGCAACCTCAAGCAA+CGG | + | 21206:592-611 | MS.gene41438:intergenic | 40.0% | |
| ! | ATAATCTCCTCATCGGTTGA+TGG | + | 21206:293-312 | MS.gene41438:intergenic | 40.0% |
| ! | ATTTTGTAGGAAGGGGATCA+AGG | - | 21206:460-479 | MS.gene41438:intron | 40.0% |
| !! | TCTGTTGATTCTCGTTACCA+TGG | - | 21206:784-803 | MS.gene41438:CDS | 40.0% |
| ATACATAGCCATCGAGATCG+CGG | + | 21206:166-185 | MS.gene41437:intergenic | 45.0% | |
| ATATCTACGTCCGTTGCTTG+AGG | - | 21206:579-598 | MS.gene41438:CDS | 45.0% | |
| CTGTGAAGGAAAACAACTGC+AGG | + | 21206:541-560 | MS.gene41438:intergenic | 45.0% | |
| GGAGGAAAGTTATGCACCAT+TGG | + | 21206:272-291 | MS.gene41438:intergenic | 45.0% | |
| ! | TTTGCGACTTGGACGGTTAA+CGG | + | 21206:248-267 | MS.gene41438:intergenic | 45.0% |
| !!! | CATTGGTTTTGCGACTTGGA+CGG | + | 21206:255-274 | MS.gene41438:intergenic | 45.0% |
| CGTCCAAGTCGCAAAACCAA+TGG | - | 21206:253-272 | MS.gene41438:intron | 50.0% | |
| CGTTCTTTGCACGTTCACCA+TGG | + | 21206:804-823 | MS.gene41438:intergenic | 50.0% | |
| CTTGAGGAGGTCGTCTGTTA+CGG | + | 21206:132-151 | MS.gene41438:intergenic | 50.0% | |
| CTTTCCTCCATCAACCGATG+AGG | - | 21206:283-302 | MS.gene41438:intron | 50.0% | |
| GCCTGAAACACGAAGACCAA+AGG | + | 21206:736-755 | MS.gene41438:intergenic | 50.0% | |
| GGAAACGTTGTACCTTGAGG+AGG | + | 21206:145-164 | MS.gene41438:intergenic | 50.0% | |
| TGTAGGAAGGGGATCAAGGA+CGG | - | 21206:464-483 | MS.gene41438:intron | 50.0% | |
| TTTCAGGCATATGCTTCGCG+AGG | - | 21206:748-767 | MS.gene41438:intron | 50.0% | |
| ! | GCCTTTGGTCTTCGTGTTTC+AGG | - | 21206:732-751 | MS.gene41438:intron | 50.0% |
| !! | ATCTCCTCATCGGTTGATGG+AGG | + | 21206:290-309 | MS.gene41438:intergenic | 50.0% |
| !!! | GCACCATTGGTTTTGCGACT+TGG | + | 21206:259-278 | MS.gene41438:intergenic | 50.0% |
| CAACGTTTCCGCGATCTCGA+TGG | - | 21206:155-174 | MS.gene41438:intron | 55.0% | |
| CGCGGAAACGTTGTACCTTG+AGG | + | 21206:148-167 | MS.gene41438:intergenic | 55.0% | |
| CGTAACAGACGACCTCCTCA+AGG | - | 21206:130-149 | MS.gene41438:CDS | 55.0% | |
| GAGGAGGTCGTCTGTTACGG+AGG | + | 21206:129-148 | MS.gene41438:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 21206 | gene | 67 | 840 | 67 | ID=MS.gene41438 |
| 21206 | mRNA | 67 | 840 | 67 | ID=MS.gene41438.t1;Parent=MS.gene41438 |
| 21206 | exon | 756 | 840 | 756 | ID=MS.gene41438.t1.exon1;Parent=MS.gene41438.t1 |
| 21206 | CDS | 756 | 840 | 756 | ID=cds.MS.gene41438.t1;Parent=MS.gene41438.t1 |
| 21206 | exon | 519 | 664 | 519 | ID=MS.gene41438.t1.exon2;Parent=MS.gene41438.t1 |
| 21206 | CDS | 519 | 664 | 519 | ID=cds.MS.gene41438.t1;Parent=MS.gene41438.t1 |
| 21206 | exon | 307 | 438 | 307 | ID=MS.gene41438.t1.exon3;Parent=MS.gene41438.t1 |
| 21206 | CDS | 307 | 438 | 307 | ID=cds.MS.gene41438.t1;Parent=MS.gene41438.t1 |
| 21206 | exon | 67 | 153 | 67 | ID=MS.gene41438.t1.exon4;Parent=MS.gene41438.t1 |
| 21206 | CDS | 67 | 153 | 67 | ID=cds.MS.gene41438.t1;Parent=MS.gene41438.t1 |
| Gene Sequence |
| Protein sequence |
