AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050122


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050122.t1 MTR_8g080230 89.744 117 12 0 1 117 1 117 3.87e-42 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGTGTGGTTGAGGAGTTTG+AGG 0.343973 4.4:+16150113 MS.gene050122:CDS
TCTCCTCCGGTGCCTCTTAT+CGG 0.348322 4.4:-16149890 None:intergenic
ACGGCGGAACGTTCAAGTTC+AGG 0.407328 4.4:+16149933 MS.gene050122:CDS
TGAGGAGTTTGAGGATCAGT+TGG 0.424110 4.4:+16150122 MS.gene050122:CDS
TTCATCAATGGTGTTGGTCA+AGG 0.463683 4.4:+16150150 MS.gene050122:CDS
AATGAAAGTTAGTGTGGTTG+AGG 0.473822 4.4:+16150104 MS.gene050122:CDS
TTGCTCACTTTGCTGTCCAC+CGG 0.486780 4.4:-16149997 None:intergenic
CAGTTGGAGTATTTCATCAA+TGG 0.487178 4.4:+16150138 MS.gene050122:CDS
CGTCGGTATTCTCCGATAAG+AGG 0.494868 4.4:+16149878 MS.gene050122:CDS
CAAGACTACAACGTCGTCTC+CGG 0.510717 4.4:+16149978 MS.gene050122:CDS
GAGTATTTCATCAATGGTGT+TGG 0.511662 4.4:+16150144 MS.gene050122:CDS
TCATCAATGGTGTTGGTCAA+GGG 0.527017 4.4:+16150151 MS.gene050122:CDS
CGGAGAATACCGACGGCGAT+CGG 0.549502 4.4:-16149870 None:intergenic
AAGCAACGGTGAAATGATAG+AGG 0.555163 4.4:+16150053 MS.gene050122:CDS
ACCGTGAAATGATTCTCCTC+CGG 0.555957 4.4:-16149903 None:intergenic
AAAGAAAATGAAAGTTAGTG+TGG 0.562354 4.4:+16150098 MS.gene050122:CDS
ACCGGAGGAGAATCATTTCA+CGG 0.579496 4.4:+16149902 MS.gene050122:CDS
TCATTTCACGGTCATCTGAA+CGG 0.584499 4.4:+16149914 MS.gene050122:CDS
GACTACAACGTCGTCTCCGG+TGG 0.584840 4.4:+16149981 MS.gene050122:CDS
TACAAAGTAGCTCGAGTGGT+GGG 0.598092 4.4:+16150028 MS.gene050122:CDS
GACGGCGATCGGAGGAGTAA+CGG 0.598155 4.4:-16149859 None:intergenic
TCGAGTGGTGGGAGAAGCAA+CGG 0.607804 4.4:+16150039 MS.gene050122:CDS
TTACAAAGTAGCTCGAGTGG+TGG 0.614453 4.4:+16150027 MS.gene050122:CDS
TATTCTCCGATAAGAGGCAC+CGG 0.616121 4.4:+16149884 MS.gene050122:CDS
GTGGACAGCAAAGTGAGCAA+TGG 0.630531 4.4:+16150000 MS.gene050122:CDS
GTTACTCCTCCGATCGCCGT+CGG 0.630592 4.4:+16149861 MS.gene050122:CDS
AGAATACCGACGGCGATCGG+AGG 0.636190 4.4:-16149867 None:intergenic
TCGTTACAAAGTAGCTCGAG+TGG 0.638320 4.4:+16150024 MS.gene050122:CDS
CTCTTATCGGAGAATACCGA+CGG 0.638821 4.4:-16149877 None:intergenic
CAAGTTCAGGCAGTGATCAG+CGG 0.651845 4.4:+16149946 MS.gene050122:CDS
GTTCAGGCAGTGATCAGCGG+CGG 0.669499 4.4:+16149949 MS.gene050122:CDS
TCTCCGATAAGAGGCACCGG+AGG 0.671014 4.4:+16149887 MS.gene050122:CDS
AATGATAGAGGTGAAAGCAG+TGG 0.733351 4.4:+16150065 MS.gene050122:CDS
TTTCACGGTCATCTGAACGG+CGG 0.762381 4.4:+16149917 MS.gene050122:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAAAATGAAAGTTAGTG+TGG + chr4.4:16150098-16150117 MS.gene050122:CDS 25.0%
AATGAAAGTTAGTGTGGTTG+AGG + chr4.4:16150104-16150123 MS.gene050122:CDS 35.0%
GAGTATTTCATCAATGGTGT+TGG + chr4.4:16150144-16150163 MS.gene050122:CDS 35.0%
! CAGTTGGAGTATTTCATCAA+TGG + chr4.4:16150138-16150157 MS.gene050122:CDS 35.0%
AAGCAACGGTGAAATGATAG+AGG + chr4.4:16150053-16150072 MS.gene050122:CDS 40.0%
AATGATAGAGGTGAAAGCAG+TGG + chr4.4:16150065-16150084 MS.gene050122:CDS 40.0%
TCATTTCACGGTCATCTGAA+CGG + chr4.4:16149914-16149933 MS.gene050122:CDS 40.0%
!! TCATCAATGGTGTTGGTCAA+GGG + chr4.4:16150151-16150170 MS.gene050122:CDS 40.0%
!! TTCATCAATGGTGTTGGTCA+AGG + chr4.4:16150150-16150169 MS.gene050122:CDS 40.0%
ACCGGAGGAGAATCATTTCA+CGG + chr4.4:16149902-16149921 MS.gene050122:CDS 45.0%
ACCGTGAAATGATTCTCCTC+CGG - chr4.4:16149906-16149925 None:intergenic 45.0%
CTCTTATCGGAGAATACCGA+CGG - chr4.4:16149880-16149899 None:intergenic 45.0%
TACAAAGTAGCTCGAGTGGT+GGG + chr4.4:16150028-16150047 MS.gene050122:CDS 45.0%
TAGTGTGGTTGAGGAGTTTG+AGG + chr4.4:16150113-16150132 MS.gene050122:CDS 45.0%
TCGTTACAAAGTAGCTCGAG+TGG + chr4.4:16150024-16150043 MS.gene050122:CDS 45.0%
TGAGGAGTTTGAGGATCAGT+TGG + chr4.4:16150122-16150141 MS.gene050122:CDS 45.0%
TTACAAAGTAGCTCGAGTGG+TGG + chr4.4:16150027-16150046 MS.gene050122:CDS 45.0%
! TATTCTCCGATAAGAGGCAC+CGG + chr4.4:16149884-16149903 MS.gene050122:CDS 45.0%
CAAGACTACAACGTCGTCTC+CGG + chr4.4:16149978-16149997 MS.gene050122:CDS 50.0%
CAAGTTCAGGCAGTGATCAG+CGG + chr4.4:16149946-16149965 MS.gene050122:CDS 50.0%
CGTCGGTATTCTCCGATAAG+AGG + chr4.4:16149878-16149897 MS.gene050122:CDS 50.0%
GTGGACAGCAAAGTGAGCAA+TGG + chr4.4:16150000-16150019 MS.gene050122:CDS 50.0%
TTTCACGGTCATCTGAACGG+CGG + chr4.4:16149917-16149936 MS.gene050122:CDS 50.0%
! TTGCTCACTTTGCTGTCCAC+CGG - chr4.4:16150000-16150019 None:intergenic 50.0%
TCTCCTCCGGTGCCTCTTAT+CGG - chr4.4:16149893-16149912 None:intergenic 55.0%
! ACGGCGGAACGTTCAAGTTC+AGG + chr4.4:16149933-16149952 MS.gene050122:CDS 55.0%
! TCGAGTGGTGGGAGAAGCAA+CGG + chr4.4:16150039-16150058 MS.gene050122:CDS 55.0%
AGAATACCGACGGCGATCGG+AGG - chr4.4:16149870-16149889 None:intergenic 60.0%
CGGAGAATACCGACGGCGAT+CGG - chr4.4:16149873-16149892 None:intergenic 60.0%
GACGGCGATCGGAGGAGTAA+CGG - chr4.4:16149862-16149881 None:intergenic 60.0%
GACTACAACGTCGTCTCCGG+TGG + chr4.4:16149981-16150000 MS.gene050122:CDS 60.0%
GTTACTCCTCCGATCGCCGT+CGG + chr4.4:16149861-16149880 MS.gene050122:CDS 60.0%
GTTCAGGCAGTGATCAGCGG+CGG + chr4.4:16149949-16149968 MS.gene050122:CDS 60.0%
!! TCTCCGATAAGAGGCACCGG+AGG + chr4.4:16149887-16149906 MS.gene050122:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 16149838 16150188 16149838 ID=MS.gene050122
chr4.4 mRNA 16149838 16150188 16149838 ID=MS.gene050122.t1;Parent=MS.gene050122
chr4.4 exon 16149838 16150188 16149838 ID=MS.gene050122.t1.exon1;Parent=MS.gene050122.t1
chr4.4 CDS 16149838 16150188 16149838 ID=cds.MS.gene050122.t1;Parent=MS.gene050122.t1
Gene Sequence

>MS.gene050122

ATGTTCGCAGTGAAGTCTAATCCGTTACTCCTCCGATCGCCGTCGGTATTCTCCGATAAGAGGCACCGGAGGAGAATCATTTCACGGTCATCTGAACGGCGGAACGTTCAAGTTCAGGCAGTGATCAGCGGCGGAGACAGCAAGACTACAACGTCGTCTCCGGTGGACAGCAAAGTGAGCAATGGCTCGTTACAAAGTAGCTCGAGTGGTGGGAGAAGCAACGGTGAAATGATAGAGGTGAAAGCAGTGGTGATGATAAGAAAGAAAATGAAAGTTAGTGTGGTTGAGGAGTTTGAGGATCAGTTGGAGTATTTCATCAATGGTGTTGGTCAAGGGATACAGATTCAGCTT

Protein sequence

>MS.gene050122.t1

MFAVKSNPLLLRSPSVFSDKRHRRRIISRSSERRNVQVQAVISGGDSKTTTSSPVDSKVSNGSLQSSSSGGRSNGEMIEVKAVVMIRKKMKVSVVEEFEDQLEYFINGVGQGIQIQL