Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42263.t1 | XP_003603750.2 | 97.2 | 217 | 3 | 1 | 1 | 217 | 1 | 214 | 2.60E-90 | 341.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42263.t1 | F4I0N3 | 36.7 | 218 | 95 | 8 | 8 | 210 | 1 | 190 | 1.7e-18 | 94.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42263.t1 | G7J4R0 | 97.2 | 217 | 3 | 1 | 1 | 217 | 1 | 214 | 1.9e-90 | 341.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054118 | MS.gene42263 | 0.80873 | 2.68E-50 | -1.69E-46 |
| MS.gene058909 | MS.gene42263 | 0.821702 | 3.51E-53 | -1.69E-46 |
| MS.gene058912 | MS.gene42263 | 0.818686 | 1.72E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42263.t1 | MTR_3g113810 | 97.235 | 217 | 3 | 1 | 1 | 217 | 1 | 214 | 5.95e-153 | 423 |
| MS.gene42263.t1 | MTR_5g021990 | 45.946 | 222 | 102 | 10 | 1 | 216 | 1 | 210 | 3.97e-46 | 152 |
| MS.gene42263.t1 | MTR_4g040170 | 96.970 | 66 | 2 | 0 | 152 | 217 | 1 | 66 | 2.28e-42 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42263.t1 | AT1G75720 | 43.519 | 216 | 83 | 8 | 8 | 210 | 1 | 190 | 3.24e-36 | 126 |
| MS.gene42263.t1 | AT1G75720 | 43.981 | 216 | 84 | 9 | 8 | 210 | 1 | 192 | 9.01e-36 | 125 |
| MS.gene42263.t1 | AT3G51220 | 34.951 | 206 | 103 | 6 | 15 | 212 | 4 | 186 | 4.01e-27 | 102 |
| MS.gene42263.t1 | AT2G17940 | 33.673 | 196 | 119 | 4 | 16 | 205 | 13 | 203 | 2.00e-23 | 93.6 |
| MS.gene42263.t1 | AT1G75720 | 43.791 | 153 | 57 | 6 | 71 | 210 | 12 | 148 | 2.07e-20 | 84.3 |
Find 38 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGTCTATCTCTGTATTTC+TGG | 0.104808 | 3.2:+90271886 | None:intergenic |
| CATAATGGAGCAAATGAAAA+TGG | 0.193439 | 3.2:-90270896 | MS.gene42263:CDS |
| TAAAGAAGAAAGCATGTTAA+TGG | 0.275611 | 3.2:-90270801 | MS.gene42263:CDS |
| TCATTCATCATGGAACTTCT+TGG | 0.313646 | 3.2:+90270417 | None:intergenic |
| TGGAAAGGCACCCTTCTCTT+AGG | 0.324014 | 3.2:-90270505 | MS.gene42263:CDS |
| AAAGAGGCTGTTACATTATT+CGG | 0.326753 | 3.2:-90271842 | MS.gene42263:CDS |
| TAGACACAAGGGCGCCATTC+AGG | 0.343210 | 3.2:-90271871 | MS.gene42263:CDS |
| TCGTGCGTTCGAGTTCTTCC+TGG | 0.369083 | 3.2:+90270759 | None:intergenic |
| AAGTCATTAATTCCACTTAT+CGG | 0.388786 | 3.2:-90270470 | MS.gene42263:CDS |
| ATAATGGAGCAAATGAAAAT+GGG | 0.406872 | 3.2:-90270895 | MS.gene42263:CDS |
| TTCTTCTTCTTCCTAAGAGA+AGG | 0.423924 | 3.2:+90270494 | None:intergenic |
| TGATGGAGTTTCAAAAGAAA+AGG | 0.446231 | 3.2:-90270601 | MS.gene42263:CDS |
| TCATCAAGGTGTTGAGAAGT+TGG | 0.449442 | 3.2:-90270525 | MS.gene42263:CDS |
| AGGTAACATGACATGAGAAA+TGG | 0.454194 | 3.2:+90270553 | None:intergenic |
| ATTTGATGAAGAATTAATGA+TGG | 0.459950 | 3.2:-90270618 | MS.gene42263:CDS |
| TCTAATCTATAGATTCATAA+TGG | 0.462589 | 3.2:-90270911 | MS.gene42263:intron |
| TCTTCTTCTTCCTAAGAGAA+GGG | 0.487269 | 3.2:+90270495 | None:intergenic |
| ATGGGGTTGATCAGTCTTCA+AGG | 0.494065 | 3.2:-90270877 | MS.gene42263:CDS |
| TAATGTAACAGCCTCTTTGA+CGG | 0.499377 | 3.2:+90271847 | None:intergenic |
| TGAAAACGTTACAGCTGAGC+TGG | 0.505652 | 3.2:-90270852 | MS.gene42263:CDS |
| ACATTGTATGTCATCTCTCC+AGG | 0.522311 | 3.2:-90270777 | MS.gene42263:CDS |
| AAAGATGAATAAGAAGAAAG+AGG | 0.535423 | 3.2:-90271924 | None:intergenic |
| GCCTCTTTGACGGACCTGAA+TGG | 0.535948 | 3.2:+90271857 | None:intergenic |
| AAGATGAATAAGAAGAAAGA+GGG | 0.553271 | 3.2:-90271923 | MS.gene42263:CDS |
| GCCATTCAGGTCCGTCAAAG+AGG | 0.580083 | 3.2:-90271858 | MS.gene42263:CDS |
| ATGTTACCTCAAGGTCATCA+AGG | 0.584789 | 3.2:-90270539 | MS.gene42263:CDS |
| TCTCATGTCATGTTACCTCA+AGG | 0.592396 | 3.2:-90270548 | MS.gene42263:CDS |
| TGGGGTTGATCAGTCTTCAA+GGG | 0.595004 | 3.2:-90270876 | MS.gene42263:CDS |
| TCAACACCTTGATGACCTTG+AGG | 0.616815 | 3.2:+90270533 | None:intergenic |
| AAGGTGTTGAGAAGTTGGAA+AGG | 0.636189 | 3.2:-90270520 | MS.gene42263:CDS |
| TAATGGAGCAAATGAAAATG+GGG | 0.636381 | 3.2:-90270894 | MS.gene42263:CDS |
| TCATTAATTCCACTTATCGG+AGG | 0.648652 | 3.2:-90270467 | MS.gene42263:CDS |
| TGCAACTAAACTCAAACACC+AGG | 0.653524 | 3.2:-90271786 | MS.gene42263:intron |
| AAACGTTACAGCTGAGCTGG+AGG | 0.666569 | 3.2:-90270849 | MS.gene42263:CDS |
| ATGACATGAGAAATGGACGG+TGG | 0.709324 | 3.2:+90270560 | None:intergenic |
| GAAATACAGAGATAGACACA+AGG | 0.731685 | 3.2:-90271883 | MS.gene42263:CDS |
| AAATACAGAGATAGACACAA+GGG | 0.731958 | 3.2:-90271882 | MS.gene42263:CDS |
| AACATGACATGAGAAATGGA+CGG | 0.745302 | 3.2:+90270557 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAATTATTAATAATGTG+TGG | + | chr3.2:90271394-90271413 | None:intergenic | 10.0% |
| !! | ATTATAATATGTTTGTAAGT+GGG | - | chr3.2:90270738-90270757 | MS.gene42263:CDS | 15.0% |
| !! | TATAAAATATAACCATCTAA+TGG | + | chr3.2:90270824-90270843 | None:intergenic | 15.0% |
| !! | TTTAAATCATCAATTAAACT+TGG | - | chr3.2:90270649-90270668 | MS.gene42263:CDS | 15.0% |
| !!! | ATTTTATTAGAGTATCAAAA+TGG | - | chr3.2:90270605-90270624 | MS.gene42263:CDS | 15.0% |
| !! | AAATTTAATTATTGATCCGT+AGG | - | chr3.2:90271196-90271215 | MS.gene42263:intron | 20.0% |
| !! | AATAAAATTGAATGATGTGA+TGG | + | chr3.2:90270593-90270612 | None:intergenic | 20.0% |
| !! | ATTTGATGAAGAATTAATGA+TGG | - | chr3.2:90271726-90271745 | MS.gene42263:intron | 20.0% |
| !! | CATTATAATATGTTTGTAAG+TGG | - | chr3.2:90270737-90270756 | MS.gene42263:CDS | 20.0% |
| !! | TACTCAAATTCTTAATCATT+TGG | - | chr3.2:90271169-90271188 | MS.gene42263:intron | 20.0% |
| !! | TCTAATCTATAGATTCATAA+TGG | - | chr3.2:90271433-90271452 | MS.gene42263:intron | 20.0% |
| !! | TTAGAGAATAGAGATTAATA+AGG | - | chr3.2:90271310-90271329 | MS.gene42263:intron | 20.0% |
| !! | TTATAATATGTTTGTAAGTG+GGG | - | chr3.2:90270739-90270758 | MS.gene42263:CDS | 20.0% |
| !!! | TAAGATGCACAAAATAATTT+TGG | + | chr3.2:90271293-90271312 | None:intergenic | 20.0% |
| !!! | TTTTACTTTTTACATACCTA+CGG | + | chr3.2:90271215-90271234 | None:intergenic | 20.0% |
| ! | AAGTCATTAATTCCACTTAT+CGG | - | chr3.2:90271874-90271893 | MS.gene42263:CDS | 25.0% |
| ! | ACTTCCTTTATTCAATTAGA+CGG | + | chr3.2:90271029-90271048 | None:intergenic | 25.0% |
| ! | GTAACTCAATCTTACAAAAT+CGG | + | chr3.2:90270783-90270802 | None:intergenic | 25.0% |
| ! | TCCAAATATTATCCATTAGA+TGG | - | chr3.2:90270809-90270828 | MS.gene42263:CDS | 25.0% |
| ! | TTAAAAAAATAATGGCCAAG+AGG | - | chr3.2:90270850-90270869 | MS.gene42263:CDS | 25.0% |
| ! | TTACTGTGTAAGATTAGTAT+CGG | - | chr3.2:90271243-90271262 | MS.gene42263:intron | 25.0% |
| !! | ACCATCTAATGGATAATATT+TGG | + | chr3.2:90270813-90270832 | None:intergenic | 25.0% |
| !! | ATAATGGAGCAAATGAAAAT+GGG | - | chr3.2:90271449-90271468 | MS.gene42263:intron | 25.0% |
| !! | ATTAGAGTATCAAAATGGTT+AGG | - | chr3.2:90270610-90270629 | MS.gene42263:CDS | 25.0% |
| !! | TAAAGAAGAAAGCATGTTAA+TGG | - | chr3.2:90271543-90271562 | MS.gene42263:intron | 25.0% |
| !! | TTCAATGTAGTCTTACAATA+TGG | - | chr3.2:90271126-90271145 | MS.gene42263:intron | 25.0% |
| !!! | AGGTATTTTTTCTAGGAAAA+AGG | - | chr3.2:90271897-90271916 | MS.gene42263:CDS | 25.0% |
| !!! | GGTATTTTTTCTAGGAAAAA+GGG | - | chr3.2:90271898-90271917 | MS.gene42263:CDS | 25.0% |
| AAAGAGGCTGTTACATTATT+CGG | - | chr3.2:90270502-90270521 | MS.gene42263:CDS | 30.0% | |
| AAATACAGAGATAGACACAA+GGG | - | chr3.2:90270462-90270481 | MS.gene42263:CDS | 30.0% | |
| CGAAACTGAAAAAAATCAAG+TGG | - | chr3.2:90271636-90271655 | MS.gene42263:intron | 30.0% | |
| GTTTCAAAGTAACATAGGAT+AGG | + | chr3.2:90270921-90270940 | None:intergenic | 30.0% | |
| TAATGGAGCAAATGAAAATG+GGG | - | chr3.2:90271450-90271469 | MS.gene42263:intron | 30.0% | |
| TGATGGAGTTTCAAAAGAAA+AGG | - | chr3.2:90271743-90271762 | MS.gene42263:intron | 30.0% | |
| TTCACCGTCTAATTGAATAA+AGG | - | chr3.2:90271022-90271041 | MS.gene42263:intron | 30.0% | |
| ! | CATAATGGAGCAAATGAAAA+TGG | - | chr3.2:90271448-90271467 | MS.gene42263:intron | 30.0% |
| ! | TCTTGAACTTTTCACTTCAA+AGG | + | chr3.2:90271707-90271726 | None:intergenic | 30.0% |
| !! | ATCCTATGTTACTTTGAAAC+AGG | - | chr3.2:90270921-90270940 | MS.gene42263:intron | 30.0% |
| !! | TCCTATGTTACTTTGAAACA+GGG | - | chr3.2:90270922-90270941 | MS.gene42263:intron | 30.0% |
| !! | TCTTACAAAATCGGTTTGTA+AGG | + | chr3.2:90270774-90270793 | None:intergenic | 30.0% |
| !!! | TTATCGGAGGTATTTTTTCT+AGG | - | chr3.2:90271890-90271909 | MS.gene42263:CDS | 30.0% |
| AAATGTCCAAGAATTCCTCT+TGG | + | chr3.2:90270868-90270887 | None:intergenic | 35.0% | |
| AACATGACATGAGAAATGGA+CGG | + | chr3.2:90271790-90271809 | None:intergenic | 35.0% | |
| ACCCTGTTTCAAAGTAACAT+AGG | + | chr3.2:90270926-90270945 | None:intergenic | 35.0% | |
| AGGAGAATCTTATTGATGGA+TGG | + | chr3.2:90270901-90270920 | None:intergenic | 35.0% | |
| AGGTAACATGACATGAGAAA+TGG | + | chr3.2:90271794-90271813 | None:intergenic | 35.0% | |
| GAAAAAATACCTCCGATAAG+TGG | + | chr3.2:90271889-90271908 | None:intergenic | 35.0% | |
| GAAATACAGAGATAGACACA+AGG | - | chr3.2:90270461-90270480 | MS.gene42263:CDS | 35.0% | |
| GGATAGGAGAATCTTATTGA+TGG | + | chr3.2:90270905-90270924 | None:intergenic | 35.0% | |
| TAATGTAACAGCCTCTTTGA+CGG | + | chr3.2:90270500-90270519 | None:intergenic | 35.0% | |
| TCATTAATTCCACTTATCGG+AGG | - | chr3.2:90271877-90271896 | MS.gene42263:CDS | 35.0% | |
| TCCTCATCATCACTTGTTTA+TGG | - | chr3.2:90271352-90271371 | MS.gene42263:intron | 35.0% | |
| TCTTCTTCTTCCTAAGAGAA+GGG | + | chr3.2:90271852-90271871 | None:intergenic | 35.0% | |
| TGTGTCTATCTCTGTATTTC+TGG | + | chr3.2:90270461-90270480 | None:intergenic | 35.0% | |
| TTCTTCTTCTTCCTAAGAGA+AGG | + | chr3.2:90271853-90271872 | None:intergenic | 35.0% | |
| ! | ACCATAAACAAGTGATGATG+AGG | + | chr3.2:90271356-90271375 | None:intergenic | 35.0% |
| !! | TTCGGTGATAAAGTTTTAGC+TGG | - | chr3.2:90270520-90270539 | MS.gene42263:CDS | 35.0% |
| ACATTGTATGTCATCTCTCC+AGG | - | chr3.2:90271567-90271586 | MS.gene42263:intron | 40.0% | |
| ATGTTACCTCAAGGTCATCA+AGG | - | chr3.2:90271805-90271824 | MS.gene42263:CDS | 40.0% | |
| TAATGGCCAAGAGGAATTCT+TGG | - | chr3.2:90270859-90270878 | MS.gene42263:CDS | 40.0% | |
| TCTCATGTCATGTTACCTCA+AGG | - | chr3.2:90271796-90271815 | MS.gene42263:CDS | 40.0% | |
| TGCAACTAAACTCAAACACC+AGG | - | chr3.2:90270558-90270577 | MS.gene42263:CDS | 40.0% | |
| TGTCGTATATCACAGAACGT+AGG | + | chr3.2:90270719-90270738 | None:intergenic | 40.0% | |
| ! | ATGTGATGGTGTATAGAACC+TGG | + | chr3.2:90270579-90270598 | None:intergenic | 40.0% |
| !! | AAGGTGTTGAGAAGTTGGAA+AGG | - | chr3.2:90271824-90271843 | MS.gene42263:CDS | 40.0% |
| !! | TCATCAAGGTGTTGAGAAGT+TGG | - | chr3.2:90271819-90271838 | MS.gene42263:CDS | 40.0% |
| ATGACATGAGAAATGGACGG+TGG | + | chr3.2:90271787-90271806 | None:intergenic | 45.0% | |
| TGAAAACGTTACAGCTGAGC+TGG | - | chr3.2:90271492-90271511 | MS.gene42263:intron | 45.0% | |
| ! | ATGGGGTTGATCAGTCTTCA+AGG | - | chr3.2:90271467-90271486 | MS.gene42263:intron | 45.0% |
| ! | TCAACACCTTGATGACCTTG+AGG | + | chr3.2:90271814-90271833 | None:intergenic | 45.0% |
| ! | TGGGGTTGATCAGTCTTCAA+GGG | - | chr3.2:90271468-90271487 | MS.gene42263:intron | 45.0% |
| !! | CAAAATCGGTTTGTAAGGCG+AGG | + | chr3.2:90270769-90270788 | None:intergenic | 45.0% |
| !! | TATACTTATTAAAAAAATAA+TGG | - | chr3.2:90270842-90270861 | MS.gene42263:CDS | 5.0% |
| AAACGTTACAGCTGAGCTGG+AGG | - | chr3.2:90271495-90271514 | MS.gene42263:intron | 50.0% | |
| !! | TGGAAAGGCACCCTTCTCTT+AGG | - | chr3.2:90271839-90271858 | MS.gene42263:CDS | 50.0% |
| GCCATTCAGGTCCGTCAAAG+AGG | - | chr3.2:90270486-90270505 | MS.gene42263:CDS | 55.0% | |
| ! | GCCTCTTTGACGGACCTGAA+TGG | + | chr3.2:90270490-90270509 | None:intergenic | 55.0% |
| ! | TCGTGCGTTCGAGTTCTTCC+TGG | + | chr3.2:90271588-90271607 | None:intergenic | 55.0% |
| !! | TAGACACAAGGGCGCCATTC+AGG | - | chr3.2:90270473-90270492 | MS.gene42263:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 90270424 | 90271942 | 90270424 | ID=MS.gene42263 |
| chr3.2 | mRNA | 90270424 | 90271942 | 90270424 | ID=MS.gene42263.t1;Parent=MS.gene42263 |
| chr3.2 | exon | 90271787 | 90271942 | 90271787 | ID=MS.gene42263.t1.exon1;Parent=MS.gene42263.t1 |
| chr3.2 | CDS | 90271787 | 90271942 | 90271787 | ID=cds.MS.gene42263.t1;Parent=MS.gene42263.t1 |
| chr3.2 | exon | 90270424 | 90270921 | 90270424 | ID=MS.gene42263.t1.exon2;Parent=MS.gene42263.t1 |
| chr3.2 | CDS | 90270424 | 90270921 | 90270424 | ID=cds.MS.gene42263.t1;Parent=MS.gene42263.t1 |
| Gene Sequence |
| Protein sequence |
