AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42334


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42334.t1 MTR_3g111950 89.568 278 22 1 1 278 1 271 1.05e-175 485
MS.gene42334.t1 MTR_1g022365 44.495 218 75 12 55 238 358 563 3.34e-35 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42334.t1 AT1G75260 35.714 224 96 8 77 270 302 507 1.45e-30 119

Find 73 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTTCTCTTCTTCCTCTT+TGG 0.178218 3.2:-89342849 None:intergenic
TTTGATACATTCATGATATC+TGG 0.259780 3.2:-89342608 None:intergenic
CCATGACGACGAGGTTTATC+AGG 0.272263 3.2:-89343136 None:intergenic
GTTGTTTGAGGTTCAGAAAT+TGG 0.272319 3.2:-89342512 None:intergenic
TTGTTTGAGGTTCAGAAATT+GGG 0.306622 3.2:-89342511 None:intergenic
ACGATGCCTGAGAGGACTTC+TGG 0.355316 3.2:+89343084 MS.gene42334:CDS
ATCAAAGTTTCCTTCACAAT+TGG 0.357674 3.2:+89342460 MS.gene42334:CDS
GGGGTAGGAGGAGGCTGTGG+TGG 0.359212 3.2:-89342491 None:intergenic
AGGAGGCTGTGGTGGGGAAT+AGG 0.359341 3.2:-89342483 None:intergenic
GGAAACTGTGTTGCCGTTGT+TGG 0.377101 3.2:-89342701 None:intergenic
ATGGTGCAAGGTTCAATAAA+TGG 0.383558 3.2:+89342926 MS.gene42334:CDS
TTTCTCTCCAGAGTTGTTTG+AGG 0.407441 3.2:-89342524 None:intergenic
GGTAGGAGGAGGCTGTGGTG+GGG 0.412575 3.2:-89342489 None:intergenic
GGAGATATTAGAAAGGATAA+TGG 0.413287 3.2:+89343175 MS.gene42334:CDS
CCTTGTTTAACTTGAGGCTC+AGG 0.414918 3.2:-89342986 None:intergenic
CTGAGGTGAAAACGGTTAGC+AGG 0.440088 3.2:+89343026 MS.gene42334:CDS
CCGCGAGGCTGAGGTGAAAA+CGG 0.442470 3.2:+89343018 MS.gene42334:CDS
GAGATATTAGAAAGGATAAT+GGG 0.443491 3.2:+89343176 MS.gene42334:CDS
GAAACTGTGTTGCCGTTGTT+GGG 0.443845 3.2:-89342700 None:intergenic
TTTCCATGTTGATTGGGAAT+GGG 0.447769 3.2:-89342798 None:intergenic
CGAGGTTTATCAGGATTGTC+AGG 0.449924 3.2:-89343127 None:intergenic
GAGTATGAATAATTCATTGA+TGG 0.450670 3.2:+89342907 MS.gene42334:CDS
TGGATAGCAGGTTCTTCCAT+TGG 0.456218 3.2:+89342413 MS.gene42334:CDS
GGGTAGGAGGAGGCTGTGGT+GGG 0.475555 3.2:-89342490 None:intergenic
GGTTCAGAAATTGGGGTAGG+AGG 0.481617 3.2:-89342503 None:intergenic
TTCTGAACCTCAAACAACTC+TGG 0.485643 3.2:+89342517 MS.gene42334:CDS
AGGCATCGTCTTCGAACCAT+TGG 0.487872 3.2:-89343070 None:intergenic
ATGGAAGAACCTGCTATCCA+TGG 0.493699 3.2:-89342410 None:intergenic
TCTAAGCCTTGTTTAACTTG+AGG 0.493815 3.2:-89342992 None:intergenic
TTTAGAATCCCATGGATAGC+AGG 0.494336 3.2:+89342401 MS.gene42334:CDS
GAAGAAGAGAAAGATGAAGC+GGG 0.496075 3.2:+89342857 MS.gene42334:CDS
ATTGGGGTAGGAGGAGGCTG+TGG 0.497179 3.2:-89342494 None:intergenic
TCAGAAATTGGGGTAGGAGG+AGG 0.502119 3.2:-89342500 None:intergenic
CATGCAAAATAGTACTATGA+AGG 0.511507 3.2:+89342637 MS.gene42334:CDS
CTTAGAAAACCGCGAGGCTG+AGG 0.522212 3.2:+89343009 MS.gene42334:CDS
TGTTTGAGGTTCAGAAATTG+GGG 0.522313 3.2:-89342510 None:intergenic
AGAGAAAGATGAAGCGGGAA+AGG 0.526730 3.2:+89342862 MS.gene42334:CDS
TGAGGTTCAGAAATTGGGGT+AGG 0.527237 3.2:-89342506 None:intergenic
AACAGTCAGTGTGGTAACTC+TGG 0.529273 3.2:+89342736 MS.gene42334:CDS
GTTCCACCAGAAGTCCTCTC+AGG 0.530890 3.2:-89343090 None:intergenic
GGAAGAATGACACGAACACC+AGG 0.533299 3.2:-89342956 None:intergenic
ACTAGGGTTTGATGTGGCAA+TGG 0.537188 3.2:-89342558 None:intergenic
GGAGCATCAATGCATGTGGC+AGG 0.545220 3.2:+89342773 MS.gene42334:CDS
AATAATTCATTGATGGTGCA+AGG 0.549285 3.2:+89342914 MS.gene42334:CDS
CTTTGGTTCGATGTATGTTG+TGG 0.551301 3.2:-89342832 None:intergenic
GGAAGAAGAGAAAGATGAAG+CGG 0.555986 3.2:+89342856 MS.gene42334:CDS
CCTGATAAACCTCGTCGTCA+TGG 0.559548 3.2:+89343136 MS.gene42334:CDS
GTCAGTGTGGTAACTCTGGC+GGG 0.561361 3.2:+89342740 MS.gene42334:CDS
ATCGTCTTCGAACCATTGGT+TGG 0.575151 3.2:-89343066 None:intergenic
AAACGGTTAGCAGGGTAGAA+AGG 0.582759 3.2:+89343035 MS.gene42334:CDS
TCGATGAGACTGAAATCCAA+TGG 0.583103 3.2:-89342429 None:intergenic
AAGGTTAACTTACCAACCAA+TGG 0.584310 3.2:+89343054 MS.gene42334:CDS
CCTGAGCCTCAAGTTAAACA+AGG 0.592796 3.2:+89342986 MS.gene42334:CDS
CAACATACATCGAACCAAAG+AGG 0.604429 3.2:+89342835 MS.gene42334:CDS
TGAGGTGAAAACGGTTAGCA+GGG 0.605686 3.2:+89343027 MS.gene42334:CDS
TCAATAAATGGAAGAGACCC+TGG 0.606453 3.2:+89342938 MS.gene42334:CDS
GGGGAATAGGCCAATTGTGA+AGG 0.609468 3.2:-89342470 None:intergenic
TGGAAGAACCTGCTATCCAT+GGG 0.610267 3.2:-89342409 None:intergenic
CAGCTGCGGAGATATTAGAA+AGG 0.615947 3.2:+89343168 MS.gene42334:CDS
ATGCCTGAGAGGACTTCTGG+TGG 0.618575 3.2:+89343087 MS.gene42334:CDS
TTGTCAGGATCAGAATCACT+TGG 0.633125 3.2:-89343112 None:intergenic
GTTCCCATTCCCAATCAACA+TGG 0.633206 3.2:+89342795 MS.gene42334:CDS
GTTCGAAGACGATGCCTGAG+AGG 0.633510 3.2:+89343076 MS.gene42334:CDS
AGTCAGTGTGGTAACTCTGG+CGG 0.637462 3.2:+89342739 MS.gene42334:CDS
CAGAGGAGCATCAATGCATG+TGG 0.658397 3.2:+89342769 MS.gene42334:CDS
CATGGCTGCAAAGTCAGCTG+CGG 0.666343 3.2:+89343154 MS.gene42334:CDS
ACTTTGCAGCCATGACGACG+AGG 0.688398 3.2:-89343145 None:intergenic
AGCCACGGTGAACCCAACAA+CGG 0.710101 3.2:+89342688 MS.gene42334:CDS
AGATGACAAAACAGTCAGTG+TGG 0.725694 3.2:+89342727 MS.gene42334:CDS
TGCCGTTGTTGGGTTCACCG+TGG 0.729315 3.2:-89342690 None:intergenic
GAAGAATGACACGAACACCA+GGG 0.740678 3.2:-89342955 None:intergenic
ACTCTGGCGGGTGACAACAG+AGG 0.755128 3.2:+89342752 MS.gene42334:CDS
ACAAGGCTTAGAAAACCGCG+AGG 0.788885 3.2:+89343003 MS.gene42334:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGATGTTTTTTTCACT+AGG - chr3.2:89342578-89342597 None:intergenic 20.0%
!!! ATTTGATGTTTTTTTCACTA+GGG - chr3.2:89342577-89342596 None:intergenic 20.0%
! GAGATATTAGAAAGGATAAT+GGG + chr3.2:89343176-89343195 MS.gene42334:CDS 25.0%
! GAGTATGAATAATTCATTGA+TGG + chr3.2:89342907-89342926 MS.gene42334:CDS 25.0%
! TTTGATACATTCATGATATC+TGG - chr3.2:89342611-89342630 None:intergenic 25.0%
!! TTCATAGTACTATTTTGCAT+GGG - chr3.2:89342638-89342657 None:intergenic 25.0%
ATCAAAGTTTCCTTCACAAT+TGG + chr3.2:89342460-89342479 MS.gene42334:CDS 30.0%
CATGCAAAATAGTACTATGA+AGG + chr3.2:89342637-89342656 MS.gene42334:CDS 30.0%
GGAGATATTAGAAAGGATAA+TGG + chr3.2:89343175-89343194 MS.gene42334:CDS 30.0%
! CTTCATAGTACTATTTTGCA+TGG - chr3.2:89342639-89342658 None:intergenic 30.0%
! TCATAGTACTATTTTGCATG+GGG - chr3.2:89342637-89342656 None:intergenic 30.0%
! TTGTTTGAGGTTCAGAAATT+GGG - chr3.2:89342514-89342533 None:intergenic 30.0%
! TTTTGCTAATAAAGTAGCCA+CGG + chr3.2:89342673-89342692 MS.gene42334:CDS 30.0%
!! AATAATTCATTGATGGTGCA+AGG + chr3.2:89342914-89342933 MS.gene42334:CDS 30.0%
!!! AATTTCGTTTTAGAATCCCA+TGG + chr3.2:89342393-89342412 MS.gene42334:CDS 30.0%
AAGGTTAACTTACCAACCAA+TGG + chr3.2:89343054-89343073 MS.gene42334:CDS 35.0%
ATCTTTCTCTTCTTCCTCTT+TGG - chr3.2:89342852-89342871 None:intergenic 35.0%
ATGGTGCAAGGTTCAATAAA+TGG + chr3.2:89342926-89342945 MS.gene42334:CDS 35.0%
CCCAATCAACATGGAAAAAA+GGG + chr3.2:89342804-89342823 MS.gene42334:CDS 35.0%
TCCCAATCAACATGGAAAAA+AGG + chr3.2:89342803-89342822 MS.gene42334:CDS 35.0%
! CATAGTACTATTTTGCATGG+GGG - chr3.2:89342636-89342655 None:intergenic 35.0%
! GTTGTTTGAGGTTCAGAAAT+TGG - chr3.2:89342515-89342534 None:intergenic 35.0%
! TGTTTGAGGTTCAGAAATTG+GGG - chr3.2:89342513-89342532 None:intergenic 35.0%
! TTTCCATGTTGATTGGGAAT+GGG - chr3.2:89342801-89342820 None:intergenic 35.0%
! TTTTTCACTAGGGTTTGATG+TGG - chr3.2:89342567-89342586 None:intergenic 35.0%
!! TCTAAGCCTTGTTTAACTTG+AGG - chr3.2:89342995-89343014 None:intergenic 35.0%
!! TTTTCCATGTTGATTGGGAA+TGG - chr3.2:89342802-89342821 None:intergenic 35.0%
!!! ACCCTTTTTTCCATGTTGAT+TGG - chr3.2:89342808-89342827 None:intergenic 35.0%
!!! CCCTTTTTTCCATGTTGATT+GGG - chr3.2:89342807-89342826 None:intergenic 35.0%
AGATGACAAAACAGTCAGTG+TGG + chr3.2:89342727-89342746 MS.gene42334:CDS 40.0%
CAACATACATCGAACCAAAG+AGG + chr3.2:89342835-89342854 MS.gene42334:CDS 40.0%
GAAGAAGAGAAAGATGAAGC+GGG + chr3.2:89342857-89342876 MS.gene42334:CDS 40.0%
GGAAGAAGAGAAAGATGAAG+CGG + chr3.2:89342856-89342875 MS.gene42334:CDS 40.0%
TCAATAAATGGAAGAGACCC+TGG + chr3.2:89342938-89342957 MS.gene42334:CDS 40.0%
TCGATGAGACTGAAATCCAA+TGG - chr3.2:89342432-89342451 None:intergenic 40.0%
TTCTGAACCTCAAACAACTC+TGG + chr3.2:89342517-89342536 MS.gene42334:CDS 40.0%
TTGTCAGGATCAGAATCACT+TGG - chr3.2:89343115-89343134 None:intergenic 40.0%
TTTCTCTCCAGAGTTGTTTG+AGG - chr3.2:89342527-89342546 None:intergenic 40.0%
! CTGACTGTTTTGTCATCTGT+CGG - chr3.2:89342725-89342744 None:intergenic 40.0%
! CTTTGGTTCGATGTATGTTG+TGG - chr3.2:89342835-89342854 None:intergenic 40.0%
! TTTAGAATCCCATGGATAGC+AGG + chr3.2:89342401-89342420 MS.gene42334:CDS 40.0%
AAACGGTTAGCAGGGTAGAA+AGG + chr3.2:89343035-89343054 MS.gene42334:CDS 45.0%
AACAGTCAGTGTGGTAACTC+TGG + chr3.2:89342736-89342755 MS.gene42334:CDS 45.0%
ACTAGGGTTTGATGTGGCAA+TGG - chr3.2:89342561-89342580 None:intergenic 45.0%
AGAGAAAGATGAAGCGGGAA+AGG + chr3.2:89342862-89342881 MS.gene42334:CDS 45.0%
ATCGTCTTCGAACCATTGGT+TGG - chr3.2:89343069-89343088 None:intergenic 45.0%
ATGGAAGAACCTGCTATCCA+TGG - chr3.2:89342413-89342432 None:intergenic 45.0%
CAGCTGCGGAGATATTAGAA+AGG + chr3.2:89343168-89343187 MS.gene42334:CDS 45.0%
CCTGAGCCTCAAGTTAAACA+AGG + chr3.2:89342986-89343005 MS.gene42334:CDS 45.0%
CGAGGTTTATCAGGATTGTC+AGG - chr3.2:89343130-89343149 None:intergenic 45.0%
GAAACTGTGTTGCCGTTGTT+GGG - chr3.2:89342703-89342722 None:intergenic 45.0%
GAAGAATGACACGAACACCA+GGG - chr3.2:89342958-89342977 None:intergenic 45.0%
GTTCCCATTCCCAATCAACA+TGG + chr3.2:89342795-89342814 MS.gene42334:CDS 45.0%
TGAGGTGAAAACGGTTAGCA+GGG + chr3.2:89343027-89343046 MS.gene42334:CDS 45.0%
TGAGGTTCAGAAATTGGGGT+AGG - chr3.2:89342509-89342528 None:intergenic 45.0%
TGGAAGAACCTGCTATCCAT+GGG - chr3.2:89342412-89342431 None:intergenic 45.0%
TGGATAGCAGGTTCTTCCAT+TGG + chr3.2:89342413-89342432 MS.gene42334:CDS 45.0%
! AACTTGAGGCTCAGGCTTTT+CGG - chr3.2:89342981-89343000 None:intergenic 45.0%
!! CCTTGTTTAACTTGAGGCTC+AGG - chr3.2:89342989-89343008 None:intergenic 45.0%
ACAAGGCTTAGAAAACCGCG+AGG + chr3.2:89343003-89343022 MS.gene42334:CDS 50.0%
AGTCAGTGTGGTAACTCTGG+CGG + chr3.2:89342739-89342758 MS.gene42334:CDS 50.0%
CCATGACGACGAGGTTTATC+AGG - chr3.2:89343139-89343158 None:intergenic 50.0%
CCTGATAAACCTCGTCGTCA+TGG + chr3.2:89343136-89343155 MS.gene42334:CDS 50.0%
CTGAGGTGAAAACGGTTAGC+AGG + chr3.2:89343026-89343045 MS.gene42334:CDS 50.0%
GGAAACTGTGTTGCCGTTGT+TGG - chr3.2:89342704-89342723 None:intergenic 50.0%
GGAAGAATGACACGAACACC+AGG - chr3.2:89342959-89342978 None:intergenic 50.0%
GGGGAATAGGCCAATTGTGA+AGG - chr3.2:89342473-89342492 None:intergenic 50.0%
GGTTCAGAAATTGGGGTAGG+AGG - chr3.2:89342506-89342525 None:intergenic 50.0%
TCAGAAATTGGGGTAGGAGG+AGG - chr3.2:89342503-89342522 None:intergenic 50.0%
! ACTTGAGGCTCAGGCTTTTC+GGG - chr3.2:89342980-89342999 None:intergenic 50.0%
! AGGCATCGTCTTCGAACCAT+TGG - chr3.2:89343073-89343092 None:intergenic 50.0%
!! CAGAGGAGCATCAATGCATG+TGG + chr3.2:89342769-89342788 MS.gene42334:CDS 50.0%
ACGATGCCTGAGAGGACTTC+TGG + chr3.2:89343084-89343103 MS.gene42334:CDS 55.0%
ACTTTGCAGCCATGACGACG+AGG - chr3.2:89343148-89343167 None:intergenic 55.0%
AGCCACGGTGAACCCAACAA+CGG + chr3.2:89342688-89342707 MS.gene42334:CDS 55.0%
ATGCCTGAGAGGACTTCTGG+TGG + chr3.2:89343087-89343106 MS.gene42334:CDS 55.0%
CATGGCTGCAAAGTCAGCTG+CGG + chr3.2:89343154-89343173 MS.gene42334:CDS 55.0%
CTTAGAAAACCGCGAGGCTG+AGG + chr3.2:89343009-89343028 MS.gene42334:CDS 55.0%
GTCAGTGTGGTAACTCTGGC+GGG + chr3.2:89342740-89342759 MS.gene42334:CDS 55.0%
GTTCCACCAGAAGTCCTCTC+AGG - chr3.2:89343093-89343112 None:intergenic 55.0%
GTTCGAAGACGATGCCTGAG+AGG + chr3.2:89343076-89343095 MS.gene42334:CDS 55.0%
! GGAGCATCAATGCATGTGGC+AGG + chr3.2:89342773-89342792 MS.gene42334:CDS 55.0%
ATTGGGGTAGGAGGAGGCTG+TGG - chr3.2:89342497-89342516 None:intergenic 60.0%
CCGCGAGGCTGAGGTGAAAA+CGG + chr3.2:89343018-89343037 MS.gene42334:CDS 60.0%
TGCCGTTGTTGGGTTCACCG+TGG - chr3.2:89342693-89342712 None:intergenic 60.0%
! ACTCTGGCGGGTGACAACAG+AGG + chr3.2:89342752-89342771 MS.gene42334:CDS 60.0%
! AGGAGGCTGTGGTGGGGAAT+AGG - chr3.2:89342486-89342505 None:intergenic 60.0%
! CCGTTTTCACCTCAGCCTCG+CGG - chr3.2:89343021-89343040 None:intergenic 60.0%
GGGTAGGAGGAGGCTGTGGT+GGG - chr3.2:89342493-89342512 None:intergenic 65.0%
GGTAGGAGGAGGCTGTGGTG+GGG - chr3.2:89342492-89342511 None:intergenic 65.0%
GGGGTAGGAGGAGGCTGTGG+TGG - chr3.2:89342494-89342513 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 89342371 89343207 89342371 ID=MS.gene42334
chr3.2 mRNA 89342371 89343207 89342371 ID=MS.gene42334.t1;Parent=MS.gene42334
chr3.2 exon 89342371 89343207 89342371 ID=MS.gene42334.t1.exon1;Parent=MS.gene42334.t1
chr3.2 CDS 89342371 89343207 89342371 ID=cds.MS.gene42334.t1;Parent=MS.gene42334.t1
Gene Sequence

>MS.gene42334

ATGTCAGAGCAAAAAAAGAAGCAATTTCGTTTTAGAATCCCATGGATAGCAGGTTCTTCCATTGGATTTCAGTCTCATCGAAAACAAAGATCAAAGTTTCCTTCACAATTGGCCTATTCCCCACCACAGCCTCCTCCTACCCCAATTTCTGAACCTCAAACAACTCTGGAGAGAAACATGATGTTTGCCATTGCCACATCAAACCCTAGTGAAAAAAACATCAAATTAGTTGCAGATCCAGATATCATGAATGTATCAAAGTCCCCCATGCAAAATAGTACTATGAAGGATGATGCAACAAATTTTGCTAATAAAGTAGCCACGGTGAACCCAACAACGGCAACACAGTTTCCGACAGATGACAAAACAGTCAGTGTGGTAACTCTGGCGGGTGACAACAGAGGAGCATCAATGCATGTGGCAGGTTCCCATTCCCAATCAACATGGAAAAAAGGGTCAATCCACAACATACATCGAACCAAAGAGGAAGAAGAGAAAGATGAAGCGGGAAAGGCATATGTGAACAGCAACATACAGAGTATGAATAATTCATTGATGGTGCAAGGTTCAATAAATGGAAGAGACCCTGGTGTTCGTGTCATTCTTCCCGAAAAGCCTGAGCCTCAAGTTAAACAAGGCTTAGAAAACCGCGAGGCTGAGGTGAAAACGGTTAGCAGGGTAGAAAGGTTAACTTACCAACCAATGGTTCGAAGACGATGCCTGAGAGGACTTCTGGTGGAACCAAGTGATTCTGATCCTGACAATCCTGATAAACCTCGTCGTCATGGCTGCAAAGTCAGCTGCGGAGATATTAGAAAGGATAATGGGATTCTGTAA

Protein sequence

>MS.gene42334.t1

MSEQKKKQFRFRIPWIAGSSIGFQSHRKQRSKFPSQLAYSPPQPPPTPISEPQTTLERNMMFAIATSNPSEKNIKLVADPDIMNVSKSPMQNSTMKDDATNFANKVATVNPTTATQFPTDDKTVSVVTLAGDNRGASMHVAGSHSQSTWKKGSIHNIHRTKEEEEKDEAGKAYVNSNIQSMNNSLMVQGSINGRDPGVRVILPEKPEPQVKQGLENREAEVKTVSRVERLTYQPMVRRRCLRGLLVEPSDSDPDNPDKPRRHGCKVSCGDIRKDNGIL