Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42652.t1 | XP_024635939.1 | 95 | 139 | 7 | 0 | 1 | 139 | 216 | 354 | 1.80E-65 | 258.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42652.t1 | G7J953 | 95.0 | 139 | 7 | 0 | 1 | 139 | 216 | 354 | 1.3e-65 | 258.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049596 | MS.gene42652 | 0.806402 | 8.37E-50 | -1.69E-46 |
| MS.gene05124 | MS.gene42652 | 0.805858 | 1.09E-49 | -1.69E-46 |
| MS.gene053416 | MS.gene42652 | 0.802313 | 5.95E-49 | -1.69E-46 |
| MS.gene06093 | MS.gene42652 | 0.811933 | 5.46E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42652.t1 | MTR_3g105470 | 94.964 | 139 | 7 | 0 | 1 | 139 | 216 | 354 | 9.24e-85 | 261 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42652.t1 | AT2G45700 | 44.286 | 140 | 66 | 4 | 1 | 139 | 277 | 405 | 3.69e-21 | 88.6 |
| MS.gene42652.t1 | AT2G45700 | 44.286 | 140 | 66 | 4 | 1 | 139 | 277 | 405 | 3.88e-21 | 88.6 |
Find 32 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGTTGCAATTTGCCTTTC+TGG | 0.032332 | 3.3:-86633457 | None:intergenic |
| TTCTGGTTTCTAATCCTTCT+AGG | 0.191762 | 3.3:-86633440 | None:intergenic |
| CCTTGTATGGCACACCATTT+AGG | 0.239725 | 3.3:-86633680 | None:intergenic |
| GTAATTAATTTGTTTGCTGC+TGG | 0.388311 | 3.3:-86633515 | None:intergenic |
| GTCGTTTCATTTCGTTCTAC+AGG | 0.388709 | 3.3:-86633587 | None:intergenic |
| AGATTCGCGACATTCCTAAA+TGG | 0.397110 | 3.3:+86633666 | MS.gene42652:CDS |
| ATGCTGAGGTTGAGCCTAGA+AGG | 0.419950 | 3.3:+86633426 | MS.gene42652:CDS |
| AAATCTGAGCGAAAAGTTGA+TGG | 0.430864 | 3.3:+86633482 | MS.gene42652:CDS |
| TCGGAAAGGTGTTCCTTGTA+TGG | 0.443532 | 3.3:-86633693 | None:intergenic |
| TTCCCACTGGTAGCAAATCC+CGG | 0.459567 | 3.3:-86633548 | None:intergenic |
| CCTAAATGGTGTGCCATACA+AGG | 0.467417 | 3.3:+86633680 | MS.gene42652:CDS |
| CTTAGAAGAGGAATCGCCTT+AGG | 0.471900 | 3.3:+86633368 | MS.gene42652:CDS |
| CTCGCTTGAACTTGAAGAGG+AGG | 0.497023 | 3.3:-86633393 | None:intergenic |
| TTGAAGGATCTCTTAAACAT+GGG | 0.525506 | 3.3:+86633296 | None:intergenic |
| CTTGAAGGATCTCTTAAACA+TGG | 0.529942 | 3.3:+86633295 | None:intergenic |
| TTCATTTCGTTCTACAGGAG+GGG | 0.530245 | 3.3:-86633582 | None:intergenic |
| TTTCATTTCGTTCTACAGGA+GGG | 0.539073 | 3.3:-86633583 | None:intergenic |
| CGAAATGAAACGACCACAAG+TGG | 0.550706 | 3.3:+86633596 | MS.gene42652:CDS |
| TTCCGGGATTTGCTACCAGT+GGG | 0.558013 | 3.3:+86633546 | MS.gene42652:CDS |
| TTTCTCGCTTGAACTTGAAG+AGG | 0.559015 | 3.3:-86633396 | None:intergenic |
| GTTTCATTTCGTTCTACAGG+AGG | 0.563033 | 3.3:-86633584 | None:intergenic |
| ACTTGAAGAGGAGGTTCCTA+AGG | 0.571607 | 3.3:-86633384 | None:intergenic |
| AACATGGGTATAACTGCACT+TGG | 0.574353 | 3.3:+86633311 | MS.gene42652:CDS |
| TCGATCAGACGAGCCACTTG+TGG | 0.583119 | 3.3:-86633609 | None:intergenic |
| TTTCCGGGATTTGCTACCAG+TGG | 0.590063 | 3.3:+86633545 | MS.gene42652:CDS |
| ATGTTGCTTACCACTCGGAA+AGG | 0.604789 | 3.3:-86633707 | None:intergenic |
| CGAGAAACATGAGGATGCTG+AGG | 0.613762 | 3.3:+86633412 | MS.gene42652:CDS |
| GCTCTCTCAGAACTTAGAAG+AGG | 0.616459 | 3.3:+86633356 | MS.gene42652:CDS |
| TGAGCGAAAAGTTGATGGAA+CGG | 0.620111 | 3.3:+86633487 | MS.gene42652:CDS |
| AGAAGGATTAGAAACCAGAA+AGG | 0.634777 | 3.3:+86633443 | MS.gene42652:CDS |
| AAGTTCAAGCGAGAAACATG+AGG | 0.646074 | 3.3:+86633403 | MS.gene42652:CDS |
| ACAAGGAACACCTTTCCGAG+TGG | 0.728495 | 3.3:+86633697 | MS.gene42652:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAATTACTGAATATTTTC+CGG | + | chr3.3:86633529-86633548 | MS.gene42652:CDS | 15.0% |
| !!! | TTAATTACTGAATATTTTCC+GGG | + | chr3.3:86633530-86633549 | MS.gene42652:CDS | 20.0% |
| !! | GTAATTAATTTGTTTGCTGC+TGG | - | chr3.3:86633518-86633537 | None:intergenic | 30.0% |
| !!! | AGCATGTACAATCTTTTTTC+TGG | - | chr3.3:86633339-86633358 | None:intergenic | 30.0% |
| !!! | GCATGTACAATCTTTTTTCT+GGG | - | chr3.3:86633338-86633357 | None:intergenic | 30.0% |
| AAATCTGAGCGAAAAGTTGA+TGG | + | chr3.3:86633482-86633501 | MS.gene42652:CDS | 35.0% | |
| AGAAGGATTAGAAACCAGAA+AGG | + | chr3.3:86633443-86633462 | MS.gene42652:CDS | 35.0% | |
| TTTCATTTCGTTCTACAGGA+GGG | - | chr3.3:86633586-86633605 | None:intergenic | 35.0% | |
| !! | TTCTGGTTTCTAATCCTTCT+AGG | - | chr3.3:86633443-86633462 | None:intergenic | 35.0% |
| AACATGGGTATAACTGCACT+TGG | + | chr3.3:86633311-86633330 | MS.gene42652:CDS | 40.0% | |
| AAGTTCAAGCGAGAAACATG+AGG | + | chr3.3:86633403-86633422 | MS.gene42652:CDS | 40.0% | |
| AGATTCGCGACATTCCTAAA+TGG | + | chr3.3:86633666-86633685 | MS.gene42652:CDS | 40.0% | |
| CATGTTGCAATTTGCCTTTC+TGG | - | chr3.3:86633460-86633479 | None:intergenic | 40.0% | |
| GTCGTTTCATTTCGTTCTAC+AGG | - | chr3.3:86633590-86633609 | None:intergenic | 40.0% | |
| GTTTCATTTCGTTCTACAGG+AGG | - | chr3.3:86633587-86633606 | None:intergenic | 40.0% | |
| TTCATTTCGTTCTACAGGAG+GGG | - | chr3.3:86633585-86633604 | None:intergenic | 40.0% | |
| TTTCTCGCTTGAACTTGAAG+AGG | - | chr3.3:86633399-86633418 | None:intergenic | 40.0% | |
| !! | TGAGCGAAAAGTTGATGGAA+CGG | + | chr3.3:86633487-86633506 | MS.gene42652:CDS | 40.0% |
| ACTTGAAGAGGAGGTTCCTA+AGG | - | chr3.3:86633387-86633406 | None:intergenic | 45.0% | |
| CCTAAATGGTGTGCCATACA+AGG | + | chr3.3:86633680-86633699 | MS.gene42652:CDS | 45.0% | |
| CCTTGTATGGCACACCATTT+AGG | - | chr3.3:86633683-86633702 | None:intergenic | 45.0% | |
| CGAAATGAAACGACCACAAG+TGG | + | chr3.3:86633596-86633615 | MS.gene42652:CDS | 45.0% | |
| CTTAGAAGAGGAATCGCCTT+AGG | + | chr3.3:86633368-86633387 | MS.gene42652:CDS | 45.0% | |
| GCTCTCTCAGAACTTAGAAG+AGG | + | chr3.3:86633356-86633375 | MS.gene42652:CDS | 45.0% | |
| !! | TCGGAAAGGTGTTCCTTGTA+TGG | - | chr3.3:86633696-86633715 | None:intergenic | 45.0% |
| !!! | GGCAGAAACTTTTTTCCCAC+TGG | - | chr3.3:86633564-86633583 | None:intergenic | 45.0% |
| ACAAGGAACACCTTTCCGAG+TGG | + | chr3.3:86633697-86633716 | MS.gene42652:CDS | 50.0% | |
| ATGCTGAGGTTGAGCCTAGA+AGG | + | chr3.3:86633426-86633445 | MS.gene42652:CDS | 50.0% | |
| CGAGAAACATGAGGATGCTG+AGG | + | chr3.3:86633412-86633431 | MS.gene42652:CDS | 50.0% | |
| CTCGCTTGAACTTGAAGAGG+AGG | - | chr3.3:86633396-86633415 | None:intergenic | 50.0% | |
| TTCCCACTGGTAGCAAATCC+CGG | - | chr3.3:86633551-86633570 | None:intergenic | 50.0% | |
| !! | TTCCGGGATTTGCTACCAGT+GGG | + | chr3.3:86633546-86633565 | MS.gene42652:CDS | 50.0% |
| !! | TTTCCGGGATTTGCTACCAG+TGG | + | chr3.3:86633545-86633564 | MS.gene42652:CDS | 50.0% |
| TCGATCAGACGAGCCACTTG+TGG | - | chr3.3:86633612-86633631 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 86633302 | 86633718 | 86633302 | ID=MS.gene42652 |
| chr3.3 | mRNA | 86633302 | 86633718 | 86633302 | ID=MS.gene42652.t1;Parent=MS.gene42652 |
| chr3.3 | exon | 86633302 | 86633718 | 86633302 | ID=MS.gene42652.t1.exon1;Parent=MS.gene42652.t1 |
| chr3.3 | CDS | 86633302 | 86633718 | 86633302 | ID=cds.MS.gene42652.t1;Parent=MS.gene42652.t1 |
| Gene Sequence |
| Protein sequence |
