Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42655.t1 | XP_003603305.1 | 93.9 | 495 | 30 | 0 | 1 | 495 | 1 | 495 | 4.20E-237 | 830.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42655.t1 | Q9C7G1 | 25.6 | 359 | 224 | 10 | 93 | 423 | 403 | 746 | 7.2e-20 | 100.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42655.t1 | G7J9V6 | 93.9 | 495 | 30 | 0 | 1 | 495 | 1 | 495 | 3.0e-237 | 830.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene42655 | 0.814471 | 1.51E-51 | -1.69E-46 |
| MS.gene051263 | MS.gene42655 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene051648 | MS.gene42655 | 0.802449 | 5.58E-49 | -1.69E-46 |
| MS.gene056228 | MS.gene42655 | 0.82473 | 6.89E-54 | -1.69E-46 |
| MS.gene058197 | MS.gene42655 | 0.800457 | 1.43E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42655.t1 | MTR_3g106140 | 97.172 | 495 | 14 | 0 | 1 | 495 | 1 | 495 | 0.0 | 980 |
| MS.gene42655.t1 | MTR_1g014420 | 63.985 | 522 | 154 | 7 | 1 | 495 | 1 | 515 | 0.0 | 545 |
| MS.gene42655.t1 | MTR_2g015870 | 32.124 | 386 | 231 | 6 | 110 | 495 | 86 | 440 | 9.98e-42 | 154 |
| MS.gene42655.t1 | MTR_8g080280 | 31.290 | 310 | 192 | 8 | 95 | 394 | 411 | 709 | 1.99e-29 | 122 |
| MS.gene42655.t1 | MTR_4g485520 | 30.094 | 319 | 207 | 7 | 79 | 394 | 174 | 479 | 1.39e-26 | 112 |
| MS.gene42655.t1 | MTR_4g485520 | 30.094 | 319 | 207 | 7 | 79 | 394 | 342 | 647 | 1.97e-26 | 113 |
| MS.gene42655.t1 | MTR_4g063800 | 31.488 | 289 | 178 | 8 | 108 | 391 | 351 | 624 | 2.09e-26 | 113 |
| MS.gene42655.t1 | MTR_5g079550 | 32.721 | 272 | 166 | 6 | 128 | 394 | 38 | 297 | 2.86e-26 | 110 |
| MS.gene42655.t1 | MTR_5g069340 | 34.799 | 273 | 161 | 5 | 128 | 394 | 52 | 313 | 9.57e-25 | 105 |
| MS.gene42655.t1 | MTR_1g018180 | 28.814 | 295 | 196 | 6 | 110 | 402 | 61 | 343 | 1.41e-24 | 105 |
| MS.gene42655.t1 | MTR_1g069845 | 30.137 | 292 | 188 | 7 | 107 | 394 | 317 | 596 | 6.85e-24 | 105 |
| MS.gene42655.t1 | MTR_1g069845 | 30.137 | 292 | 188 | 7 | 107 | 394 | 438 | 717 | 8.80e-24 | 105 |
| MS.gene42655.t1 | MTR_1g069845 | 30.137 | 292 | 188 | 7 | 107 | 394 | 434 | 713 | 8.92e-24 | 105 |
| MS.gene42655.t1 | MTR_3g466220 | 31.834 | 289 | 180 | 7 | 109 | 394 | 531 | 805 | 1.23e-23 | 105 |
| MS.gene42655.t1 | MTR_3g466220 | 31.834 | 289 | 180 | 7 | 109 | 394 | 552 | 826 | 1.62e-23 | 104 |
| MS.gene42655.t1 | MTR_7g059405 | 30.526 | 285 | 186 | 5 | 107 | 391 | 358 | 630 | 2.31e-23 | 103 |
| MS.gene42655.t1 | MTR_3g085610 | 29.221 | 308 | 197 | 8 | 96 | 394 | 412 | 707 | 8.26e-23 | 102 |
| MS.gene42655.t1 | MTR_7g097020 | 28.618 | 304 | 200 | 8 | 105 | 404 | 474 | 764 | 1.03e-22 | 102 |
| MS.gene42655.t1 | MTR_1g094215 | 31.973 | 294 | 186 | 6 | 108 | 399 | 160 | 441 | 3.99e-22 | 99.4 |
| MS.gene42655.t1 | MTR_3g067685 | 29.714 | 350 | 210 | 9 | 126 | 469 | 56 | 375 | 5.33e-22 | 98.2 |
| MS.gene42655.t1 | MTR_7g078330 | 31.250 | 288 | 184 | 6 | 105 | 390 | 362 | 637 | 6.57e-22 | 99.8 |
| MS.gene42655.t1 | MTR_1g094215 | 31.973 | 294 | 186 | 6 | 108 | 399 | 343 | 624 | 1.29e-21 | 98.6 |
| MS.gene42655.t1 | MTR_6g004790 | 30.822 | 292 | 181 | 8 | 111 | 394 | 3 | 281 | 3.05e-19 | 89.0 |
| MS.gene42655.t1 | MTR_3g103050 | 27.063 | 303 | 201 | 9 | 110 | 405 | 51 | 340 | 1.28e-17 | 84.3 |
| MS.gene42655.t1 | MTR_3g078340 | 26.299 | 308 | 208 | 6 | 89 | 394 | 196 | 486 | 2.28e-16 | 82.0 |
| MS.gene42655.t1 | MTR_2g026130 | 27.517 | 298 | 202 | 6 | 99 | 394 | 164 | 449 | 1.13e-15 | 79.7 |
| MS.gene42655.t1 | MTR_2g018010 | 26.102 | 295 | 186 | 8 | 113 | 394 | 399 | 674 | 1.63e-13 | 73.6 |
| MS.gene42655.t1 | MTR_5g083030 | 27.444 | 266 | 164 | 6 | 89 | 334 | 367 | 623 | 1.00e-12 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42655.t1 | AT4G31890 | 60.300 | 534 | 157 | 13 | 1 | 495 | 1 | 518 | 0.0 | 553 |
| MS.gene42655.t1 | AT4G31890 | 60.300 | 534 | 157 | 13 | 1 | 495 | 1 | 518 | 0.0 | 553 |
| MS.gene42655.t1 | AT4G31890 | 60.300 | 534 | 157 | 13 | 1 | 495 | 1 | 518 | 0.0 | 553 |
| MS.gene42655.t1 | AT2G25130 | 58.696 | 506 | 160 | 10 | 1 | 495 | 1 | 468 | 5.53e-179 | 510 |
| MS.gene42655.t1 | AT2G27430 | 31.877 | 389 | 233 | 8 | 110 | 495 | 79 | 438 | 1.22e-41 | 154 |
| MS.gene42655.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 247 | 627 | 1.52e-30 | 125 |
| MS.gene42655.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 247 | 627 | 1.52e-30 | 125 |
| MS.gene42655.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 439 | 819 | 2.69e-30 | 125 |
| MS.gene42655.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 436 | 816 | 3.05e-30 | 125 |
| MS.gene42655.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 436 | 816 | 3.05e-30 | 125 |
| MS.gene42655.t1 | AT1G67530 | 30.142 | 282 | 177 | 6 | 124 | 397 | 440 | 709 | 5.77e-27 | 115 |
| MS.gene42655.t1 | AT1G67530 | 30.142 | 282 | 177 | 6 | 124 | 397 | 440 | 709 | 5.77e-27 | 115 |
| MS.gene42655.t1 | AT1G67530 | 30.142 | 282 | 177 | 6 | 124 | 397 | 440 | 709 | 5.77e-27 | 115 |
| MS.gene42655.t1 | AT1G24330 | 29.316 | 307 | 200 | 6 | 118 | 418 | 434 | 729 | 1.81e-25 | 110 |
| MS.gene42655.t1 | AT1G24330 | 29.316 | 307 | 200 | 6 | 118 | 418 | 434 | 729 | 1.81e-25 | 110 |
| MS.gene42655.t1 | AT3G46510 | 33.088 | 272 | 162 | 8 | 125 | 391 | 369 | 625 | 2.20e-25 | 110 |
| MS.gene42655.t1 | AT3G54790 | 30.282 | 284 | 184 | 7 | 113 | 394 | 441 | 712 | 3.04e-25 | 110 |
| MS.gene42655.t1 | AT5G67340 | 29.653 | 317 | 207 | 7 | 79 | 394 | 396 | 697 | 3.52e-25 | 109 |
| MS.gene42655.t1 | AT5G67340 | 29.653 | 317 | 207 | 7 | 79 | 394 | 396 | 697 | 3.52e-25 | 109 |
| MS.gene42655.t1 | AT3G54790 | 30.068 | 296 | 192 | 8 | 113 | 405 | 477 | 760 | 4.50e-25 | 109 |
| MS.gene42655.t1 | AT3G54790 | 30.068 | 296 | 192 | 8 | 113 | 405 | 477 | 760 | 4.50e-25 | 109 |
| MS.gene42655.t1 | AT2G28830 | 31.023 | 303 | 187 | 10 | 109 | 403 | 284 | 572 | 1.41e-24 | 107 |
| MS.gene42655.t1 | AT3G03440 | 26.829 | 369 | 231 | 10 | 110 | 470 | 66 | 403 | 2.08e-23 | 102 |
| MS.gene42655.t1 | AT1G23030 | 29.514 | 288 | 188 | 6 | 110 | 394 | 333 | 608 | 2.15e-22 | 101 |
| MS.gene42655.t1 | AT1G27910 | 25.627 | 359 | 224 | 10 | 93 | 423 | 403 | 746 | 9.42e-22 | 99.4 |
| MS.gene42655.t1 | AT1G71020 | 30.822 | 292 | 186 | 7 | 107 | 394 | 192 | 471 | 2.09e-21 | 97.4 |
| MS.gene42655.t1 | AT1G71020 | 30.822 | 292 | 186 | 7 | 107 | 394 | 340 | 619 | 2.82e-21 | 97.8 |
| MS.gene42655.t1 | AT3G01400 | 27.152 | 302 | 200 | 8 | 110 | 408 | 64 | 348 | 3.40e-20 | 92.4 |
| MS.gene42655.t1 | AT5G58680 | 29.963 | 267 | 166 | 7 | 124 | 388 | 77 | 324 | 1.08e-19 | 90.9 |
| MS.gene42655.t1 | AT3G54850 | 31.802 | 283 | 179 | 6 | 122 | 402 | 359 | 629 | 1.34e-19 | 92.4 |
| MS.gene42655.t1 | AT4G12710 | 30.928 | 291 | 184 | 5 | 112 | 394 | 49 | 330 | 1.46e-18 | 88.2 |
| MS.gene42655.t1 | AT5G42340 | 30.263 | 304 | 198 | 6 | 90 | 391 | 361 | 652 | 2.47e-18 | 88.6 |
| MS.gene42655.t1 | AT5G65200 | 29.044 | 272 | 174 | 6 | 132 | 394 | 248 | 509 | 9.65e-18 | 86.7 |
| MS.gene42655.t1 | AT4G16490 | 28.333 | 300 | 199 | 7 | 99 | 394 | 174 | 461 | 1.34e-16 | 82.8 |
| MS.gene42655.t1 | AT5G14510 | 30.075 | 266 | 170 | 8 | 135 | 394 | 24 | 279 | 3.99e-16 | 79.7 |
| MS.gene42655.t1 | AT5G62560 | 26.298 | 289 | 200 | 5 | 109 | 394 | 235 | 513 | 4.18e-15 | 78.2 |
Find 94 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGGATGCAATCACAATTT+TGG | 0.171187 | 3.1:-84211226 | MS.gene42655:CDS |
| GATTCTAACAAACCAATAAT+TGG | 0.187713 | 3.1:-84211513 | MS.gene42655:CDS |
| GATAAAGGGAAGCAGGTTTC+TGG | 0.201909 | 3.1:-84211003 | MS.gene42655:CDS |
| GACGAATATTTCATTTGTTT+TGG | 0.222706 | 3.1:-84211370 | MS.gene42655:CDS |
| GCACCAGAGGATCCAATTAT+TGG | 0.223709 | 3.1:+84211501 | None:intergenic |
| AAAGCAGCGATTGTTAAAAT+TGG | 0.251431 | 3.1:-84211636 | MS.gene42655:CDS |
| GAAGCCTTCTCTTGACATTC+AGG | 0.257804 | 3.1:+84211177 | None:intergenic |
| CCTTTCAATTCTGAGCAATT+TGG | 0.260209 | 3.1:-84211286 | MS.gene42655:CDS |
| TCAACTGCAATTCGTCAAAA+TGG | 0.295980 | 3.1:+84210709 | None:intergenic |
| AGGAGGTAAAGAGTGTTTAA+TGG | 0.305362 | 3.1:-84210899 | MS.gene42655:CDS |
| AAAAGCTATTAGTGCTGTTA+AGG | 0.376170 | 3.1:-84211244 | MS.gene42655:CDS |
| GGCGGAGAAGCGGAAGTTAC+CGG | 0.380244 | 3.1:+84212355 | None:intergenic |
| TTTCCTTGGATTAAGTGCTT+TGG | 0.382622 | 3.1:-84211535 | MS.gene42655:CDS |
| AAGTTAAACAAGATGCTCTT+CGG | 0.386930 | 3.1:-84211419 | MS.gene42655:CDS |
| GAAGACGAAGAAGCACAAAT+AGG | 0.399369 | 3.1:+84210940 | None:intergenic |
| AGAGGCTCCGCCGCAGCTTA+AGG | 0.401079 | 3.1:+84212304 | None:intergenic |
| GCAATTGTTGCAAATTTCCT+TGG | 0.402106 | 3.1:-84211549 | MS.gene42655:CDS |
| GAGGCACACTCGCAATGCTC+GGG | 0.403694 | 3.1:-84212001 | MS.gene42655:CDS |
| GGGCAATTTCTCCTCTCGTT+GGG | 0.404224 | 3.1:-84211980 | MS.gene42655:CDS |
| AGAGAAGACGGAGAAGCTAT+TGG | 0.413753 | 3.1:-84212192 | MS.gene42655:CDS |
| GGAGATGAAGCATGTGGTTA+AGG | 0.413838 | 3.1:-84212105 | MS.gene42655:CDS |
| CACATAAAGCTTATGCTGAT+AGG | 0.414828 | 3.1:-84211134 | MS.gene42655:CDS |
| TACAATTCCTGCTTCAATCA+TGG | 0.419654 | 3.1:+84211107 | None:intergenic |
| TGCGAGTGTGCCTCTCGCTT+CGG | 0.424046 | 3.1:+84212010 | None:intergenic |
| TTGCTTGAGCTGACGCTTGT+GGG | 0.429303 | 3.1:-84211078 | MS.gene42655:CDS |
| AGAAGTGATGGTGGAAACTT+AGG | 0.433937 | 3.1:-84210979 | MS.gene42655:CDS |
| ATAAAGGGAAGCAGGTTTCT+GGG | 0.440773 | 3.1:-84211002 | MS.gene42655:CDS |
| GGGGCAATTTCTCCTCTCGT+TGG | 0.443059 | 3.1:-84211981 | MS.gene42655:CDS |
| GACGAAGCGGCGAATGGCAG+CGG | 0.449732 | 3.1:-84212063 | MS.gene42655:CDS |
| AGTTAAACAAGATGCTCTTC+GGG | 0.453105 | 3.1:-84211418 | MS.gene42655:CDS |
| CTTCTTCGCGGTGGTAATGT+CGG | 0.455884 | 3.1:+84212273 | None:intergenic |
| ACCTTGTGTTGTTGATTCAT+CGG | 0.459932 | 3.1:-84211580 | MS.gene42655:CDS |
| AAACCAATAATTGGATCCTC+TGG | 0.464190 | 3.1:-84211504 | MS.gene42655:CDS |
| TTCTCCGCGTAACTCCTTCA+CGG | 0.469664 | 3.1:+84212223 | None:intergenic |
| TAGTGCCTTGGCACAGAAAA+GGG | 0.470454 | 3.1:-84211055 | MS.gene42655:CDS |
| AGAGGCACACTCGCAATGCT+CGG | 0.474592 | 3.1:-84212002 | MS.gene42655:CDS |
| CTATATGCTTTACTCAATCT+CGG | 0.476166 | 3.1:-84211918 | MS.gene42655:CDS |
| TAAAGAGTGTTTAATGGAGG+AGG | 0.476887 | 3.1:-84210893 | MS.gene42655:CDS |
| GAAGAAGGAAGAGACATTAA+CGG | 0.484776 | 3.1:-84212126 | MS.gene42655:CDS |
| TAGGCTAGATAAAGGGAAGC+AGG | 0.493141 | 3.1:-84211010 | MS.gene42655:CDS |
| CAGAGCTAATTTCTTCTTCG+CGG | 0.495177 | 3.1:+84212261 | None:intergenic |
| ATTGGATCTTCTGAACATAC+AGG | 0.495508 | 3.1:-84212174 | MS.gene42655:CDS |
| GACGCTTGTGGGTAGTGCCT+TGG | 0.497097 | 3.1:-84211067 | MS.gene42655:CDS |
| TTTCAGAGTTCTAACTAGGA+AGG | 0.499120 | 3.1:+84211473 | None:intergenic |
| CAATTGAATCAACGTTGCTC+CGG | 0.501968 | 3.1:+84212414 | None:intergenic |
| GTAGTGCCTTGGCACAGAAA+AGG | 0.502811 | 3.1:-84211056 | MS.gene42655:CDS |
| AATGATGCGGCGGAAGGATG+AGG | 0.503235 | 3.1:+84212334 | None:intergenic |
| ATTGCTTGAGCTGACGCTTG+TGG | 0.515359 | 3.1:-84211079 | MS.gene42655:CDS |
| TTCAGAGTTCTAACTAGGAA+GGG | 0.526621 | 3.1:+84211474 | None:intergenic |
| GAGAGAAACTTACGCATCGT+TGG | 0.530546 | 3.1:+84211890 | None:intergenic |
| AGGTAAAGAGTGTTTAATGG+AGG | 0.535851 | 3.1:-84210896 | MS.gene42655:CDS |
| AATTAGCTCTGTCGCGTCGA+CGG | 0.541624 | 3.1:-84212249 | MS.gene42655:CDS |
| GGAAGGATGAGGCGGAGAAG+CGG | 0.545683 | 3.1:+84212345 | None:intergenic |
| AGGCAAGCCATGATTGAAGC+AGG | 0.546829 | 3.1:-84211114 | MS.gene42655:CDS |
| CAGGTTTCTGGGAGAAGTGA+TGG | 0.551186 | 3.1:-84210991 | MS.gene42655:CDS |
| GTCGAAAATGATGCGGCGGA+AGG | 0.563722 | 3.1:+84212328 | None:intergenic |
| AGAAGTGCGAGAAGAGAAGA+CGG | 0.565520 | 3.1:-84212204 | MS.gene42655:CDS |
| CCAAATTGCTCAGAATTGAA+AGG | 0.568365 | 3.1:+84211286 | None:intergenic |
| TGTTGATTCATCGGTTTCTG+AGG | 0.570602 | 3.1:-84211571 | MS.gene42655:CDS |
| AAATGCGGAATCGAAGAAGA+AGG | 0.572172 | 3.1:-84212141 | MS.gene42655:CDS |
| CATGATGAAAATTGTCAAGA+GGG | 0.572944 | 3.1:-84210806 | MS.gene42655:CDS |
| CGCGTCGACGGCAACCGTGA+AGG | 0.579924 | 3.1:-84212237 | MS.gene42655:CDS |
| TGGCACAGAAAAGGGCATCA+AGG | 0.580100 | 3.1:-84211047 | MS.gene42655:CDS |
| CTGCAATTCGTCAAAATGGA+AGG | 0.580211 | 3.1:+84210713 | None:intergenic |
| GAGAAATGGCTAAGTGTCAC+CGG | 0.581139 | 3.1:-84212433 | None:intergenic |
| AGTGAAGCAAAACGCTCGGA+AGG | 0.587883 | 3.1:+84210760 | None:intergenic |
| AGACTCGACGAAGCGGCGAA+TGG | 0.588292 | 3.1:-84212069 | MS.gene42655:CDS |
| AATCCAAAGCACTTAATCCA+AGG | 0.592090 | 3.1:+84211532 | None:intergenic |
| AGCTAATTTCTTCTTCGCGG+TGG | 0.599852 | 3.1:+84212264 | None:intergenic |
| TTCACCTGAATGTCAAGAGA+AGG | 0.606603 | 3.1:-84211181 | MS.gene42655:CDS |
| TTCGACGCCTTAAGCTGCGG+CGG | 0.610282 | 3.1:-84212311 | MS.gene42655:CDS |
| AGTGAGTGAAGCAAAACGCT+CGG | 0.610988 | 3.1:+84210756 | None:intergenic |
| CAATTTGGTGTCGAGTCCCG+AGG | 0.611390 | 3.1:-84211271 | MS.gene42655:CDS |
| ACATGATGAAAATTGTCAAG+AGG | 0.618956 | 3.1:-84210807 | MS.gene42655:CDS |
| TAGGAAGGGTATTGCACCAG+AGG | 0.641343 | 3.1:+84211488 | None:intergenic |
| GCGGAGAAGACTCGACGAAG+CGG | 0.643214 | 3.1:-84212076 | MS.gene42655:CDS |
| GTAATGTCGGTGGTGTCGAG+AGG | 0.644001 | 3.1:+84212286 | None:intergenic |
| CTTCGCGGTGGTAATGTCGG+TGG | 0.644496 | 3.1:+84212276 | None:intergenic |
| GATAAACTCGATAGAAGACA+TGG | 0.646492 | 3.1:-84211325 | MS.gene42655:CDS |
| AAAGAAGATTCCGAAGCGAG+AGG | 0.648370 | 3.1:-84212020 | MS.gene42655:CDS |
| AGGCACACTCGCAATGCTCG+GGG | 0.651896 | 3.1:-84212000 | MS.gene42655:CDS |
| GTTTCTGGGAGAAGTGATGG+TGG | 0.653879 | 3.1:-84210988 | MS.gene42655:CDS |
| ACCGATGAATCAACAACACA+AGG | 0.656484 | 3.1:+84211579 | None:intergenic |
| AATTTGGTGTCGAGTCCCGA+GGG | 0.656713 | 3.1:-84211270 | MS.gene42655:CDS |
| GATGCGGCGGAAGGATGAGG+CGG | 0.658978 | 3.1:+84212337 | None:intergenic |
| TTTGTGAAGACAGATGGAGG+AGG | 0.662270 | 3.1:-84210916 | MS.gene42655:CDS |
| GAATCAAGCATCCCAACGAG+AGG | 0.667890 | 3.1:+84211969 | None:intergenic |
| CATGTGGTTAAGGATTTACG+CGG | 0.683685 | 3.1:-84212095 | MS.gene42655:CDS |
| ACAGGTTCATGAAACAAATG+CGG | 0.698371 | 3.1:-84212156 | MS.gene42655:CDS |
| AGGCGTCGAAAATGATGCGG+CGG | 0.698501 | 3.1:+84212324 | None:intergenic |
| TTAAGGCGTCGAAAATGATG+CGG | 0.708242 | 3.1:+84212321 | None:intergenic |
| GGAGAAGCGGAAGTTACCGG+CGG | 0.717102 | 3.1:+84212358 | None:intergenic |
| ATTAACGGAGATGAAGCATG+TGG | 0.719445 | 3.1:-84212111 | MS.gene42655:CDS |
| GCAACCGTGAAGGAGTTACG+CGG | 0.747970 | 3.1:-84212227 | MS.gene42655:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATCATATTCTTAATTTTG+TGG | - | chr3.1:84211383-84211402 | MS.gene42655:CDS | 15.0% |
| !! | GAATATGATAAATTCATGAT+TGG | + | chr3.1:84211374-84211393 | None:intergenic | 20.0% |
| !! | GATTAAACACAAAAATTTCA+CGG | + | chr3.1:84211463-84211482 | None:intergenic | 20.0% |
| !!! | TTAATCTTTATTTGTTTCTG+TGG | - | chr3.1:84211477-84211496 | MS.gene42655:CDS | 20.0% |
| !!! | TTCATACATTTTGATGATTA+TGG | - | chr3.1:84211994-84212013 | MS.gene42655:CDS | 20.0% |
| ! | GATTCTAACAAACCAATAAT+TGG | - | chr3.1:84211638-84211657 | MS.gene42655:CDS | 25.0% |
| !! | GACGAATATTTCATTTGTTT+TGG | - | chr3.1:84211781-84211800 | MS.gene42655:intron | 25.0% |
| !!! | AATGTTTTAGGCTAGATAAA+GGG | - | chr3.1:84212134-84212153 | MS.gene42655:CDS | 25.0% |
| !!! | AATTTTTCAGAGTTCTAACT+AGG | + | chr3.1:84211685-84211704 | None:intergenic | 25.0% |
| !!! | ATTTGTTTTGGAAACTGATA+TGG | - | chr3.1:84211793-84211812 | MS.gene42655:intron | 25.0% |
| !!! | TTTTGGTTGATGTATTGAAT+TGG | - | chr3.1:84211942-84211961 | MS.gene42655:CDS | 25.0% |
| AAAGCAGCGATTGTTAAAAT+TGG | - | chr3.1:84211515-84211534 | MS.gene42655:CDS | 30.0% | |
| AAGTTAAACAAGATGCTCTT+CGG | - | chr3.1:84211732-84211751 | MS.gene42655:intron | 30.0% | |
| ACATGATGAAAATTGTCAAG+AGG | - | chr3.1:84212344-84212363 | MS.gene42655:CDS | 30.0% | |
| CATGATGAAAATTGTCAAGA+GGG | - | chr3.1:84212345-84212364 | MS.gene42655:CDS | 30.0% | |
| CTATATGCTTTACTCAATCT+CGG | - | chr3.1:84211233-84211252 | MS.gene42655:CDS | 30.0% | |
| ! | AAAAGCTATTAGTGCTGTTA+AGG | - | chr3.1:84211907-84211926 | MS.gene42655:CDS | 30.0% |
| ! | CAAGGATATTGCAATGTTTT+AGG | - | chr3.1:84212122-84212141 | MS.gene42655:CDS | 30.0% |
| ! | TAAGGATGCAATCACAATTT+TGG | - | chr3.1:84211925-84211944 | MS.gene42655:CDS | 30.0% |
| !! | CAATGTTTTAGGCTAGATAA+AGG | - | chr3.1:84212133-84212152 | MS.gene42655:CDS | 30.0% |
| AAACCAATAATTGGATCCTC+TGG | - | chr3.1:84211647-84211666 | MS.gene42655:intron | 35.0% | |
| ACAGGTTCATGAAACAAATG+CGG | - | chr3.1:84210995-84211014 | MS.gene42655:CDS | 35.0% | |
| AGTTAAACAAGATGCTCTTC+GGG | - | chr3.1:84211733-84211752 | MS.gene42655:intron | 35.0% | |
| ATTGGATCTTCTGAACATAC+AGG | - | chr3.1:84210977-84210996 | MS.gene42655:CDS | 35.0% | |
| CACATAAAGCTTATGCTGAT+AGG | - | chr3.1:84212017-84212036 | MS.gene42655:CDS | 35.0% | |
| CCAAATTGCTCAGAATTGAA+AGG | + | chr3.1:84211868-84211887 | None:intergenic | 35.0% | |
| CCTTTCAATTCTGAGCAATT+TGG | - | chr3.1:84211865-84211884 | MS.gene42655:intron | 35.0% | |
| GAAGAAGGAAGAGACATTAA+CGG | - | chr3.1:84211025-84211044 | MS.gene42655:CDS | 35.0% | |
| GATAAACTCGATAGAAGACA+TGG | - | chr3.1:84211826-84211845 | MS.gene42655:intron | 35.0% | |
| GCAATTGTTGCAAATTTCCT+TGG | - | chr3.1:84211602-84211621 | MS.gene42655:CDS | 35.0% | |
| TACAATTCCTGCTTCAATCA+TGG | + | chr3.1:84212047-84212066 | None:intergenic | 35.0% | |
| TTTCCTTGGATTAAGTGCTT+TGG | - | chr3.1:84211616-84211635 | MS.gene42655:CDS | 35.0% | |
| ! | AATCCAAAGCACTTAATCCA+AGG | + | chr3.1:84211622-84211641 | None:intergenic | 35.0% |
| ! | ACTAATAGCTTTTCTACCCT+CGG | + | chr3.1:84211900-84211919 | None:intergenic | 35.0% |
| ! | TCTTCTTTTGTGAAGACAGA+TGG | - | chr3.1:84212229-84212248 | MS.gene42655:CDS | 35.0% |
| ! | TTCAGAGTTCTAACTAGGAA+GGG | + | chr3.1:84211680-84211699 | None:intergenic | 35.0% |
| ! | TTTCAGAGTTCTAACTAGGA+AGG | + | chr3.1:84211681-84211700 | None:intergenic | 35.0% |
| !! | ACCTTGTGTTGTTGATTCAT+CGG | - | chr3.1:84211571-84211590 | MS.gene42655:CDS | 35.0% |
| !! | AGGAGGTAAAGAGTGTTTAA+TGG | - | chr3.1:84212252-84212271 | MS.gene42655:CDS | 35.0% |
| !! | AGGTAAAGAGTGTTTAATGG+AGG | - | chr3.1:84212255-84212274 | MS.gene42655:CDS | 35.0% |
| !! | TAAAGAGTGTTTAATGGAGG+AGG | - | chr3.1:84212258-84212277 | MS.gene42655:CDS | 35.0% |
| AAATGCGGAATCGAAGAAGA+AGG | - | chr3.1:84211010-84211029 | MS.gene42655:CDS | 40.0% | |
| ACCGATGAATCAACAACACA+AGG | + | chr3.1:84211575-84211594 | None:intergenic | 40.0% | |
| ATAAAGGGAAGCAGGTTTCT+GGG | - | chr3.1:84212149-84212168 | MS.gene42655:CDS | 40.0% | |
| ATTAACGGAGATGAAGCATG+TGG | - | chr3.1:84211040-84211059 | MS.gene42655:CDS | 40.0% | |
| CAATTGAATCAACGTTGCTC+CGG | + | chr3.1:84210740-84210759 | None:intergenic | 40.0% | |
| CAGAGCTAATTTCTTCTTCG+CGG | + | chr3.1:84210893-84210912 | None:intergenic | 40.0% | |
| CATGTGGTTAAGGATTTACG+CGG | - | chr3.1:84211056-84211075 | MS.gene42655:CDS | 40.0% | |
| TTCACCTGAATGTCAAGAGA+AGG | - | chr3.1:84211970-84211989 | MS.gene42655:CDS | 40.0% | |
| ! | CTAATAGCTTTTCTACCCTC+GGG | + | chr3.1:84211899-84211918 | None:intergenic | 40.0% |
| ! | GAAGACGAAGAAGCACAAAT+AGG | + | chr3.1:84212214-84212233 | None:intergenic | 40.0% |
| ! | TCTTTTGTGAAGACAGATGG+AGG | - | chr3.1:84212232-84212251 | MS.gene42655:CDS | 40.0% |
| !! | AGAAGTGATGGTGGAAACTT+AGG | - | chr3.1:84212172-84212191 | MS.gene42655:CDS | 40.0% |
| !! | TGTTGATTCATCGGTTTCTG+AGG | - | chr3.1:84211580-84211599 | MS.gene42655:CDS | 40.0% |
| !! | TTAAGGCGTCGAAAATGATG+CGG | + | chr3.1:84210833-84210852 | None:intergenic | 40.0% |
| AAAGAAGATTCCGAAGCGAG+AGG | - | chr3.1:84211131-84211150 | MS.gene42655:CDS | 45.0% | |
| AGAGAAGACGGAGAAGCTAT+TGG | - | chr3.1:84210959-84210978 | MS.gene42655:CDS | 45.0% | |
| AGCTAATTTCTTCTTCGCGG+TGG | + | chr3.1:84210890-84210909 | None:intergenic | 45.0% | |
| AGTGAGTGAAGCAAAACGCT+CGG | + | chr3.1:84212398-84212417 | None:intergenic | 45.0% | |
| GAAGCCTTCTCTTGACATTC+AGG | + | chr3.1:84211977-84211996 | None:intergenic | 45.0% | |
| GAGAGAAACTTACGCATCGT+TGG | + | chr3.1:84211264-84211283 | None:intergenic | 45.0% | |
| GATAAAGGGAAGCAGGTTTC+TGG | - | chr3.1:84212148-84212167 | MS.gene42655:CDS | 45.0% | |
| GCACCAGAGGATCCAATTAT+TGG | + | chr3.1:84211653-84211672 | None:intergenic | 45.0% | |
| GGAGATGAAGCATGTGGTTA+AGG | - | chr3.1:84211046-84211065 | MS.gene42655:CDS | 45.0% | |
| TAGGCTAGATAAAGGGAAGC+AGG | - | chr3.1:84212141-84212160 | MS.gene42655:CDS | 45.0% | |
| TAGTGCCTTGGCACAGAAAA+GGG | - | chr3.1:84212096-84212115 | MS.gene42655:CDS | 45.0% | |
| TTTGTGAAGACAGATGGAGG+AGG | - | chr3.1:84212235-84212254 | MS.gene42655:CDS | 45.0% | |
| ! | AGAAGTGCGAGAAGAGAAGA+CGG | - | chr3.1:84210947-84210966 | MS.gene42655:CDS | 45.0% |
| ! | ATTTTCGACGCCTTAAGCTG+CGG | - | chr3.1:84210837-84210856 | MS.gene42655:CDS | 45.0% |
| AGGCAAGCCATGATTGAAGC+AGG | - | chr3.1:84212037-84212056 | MS.gene42655:CDS | 50.0% | |
| AGTGAAGCAAAACGCTCGGA+AGG | + | chr3.1:84212394-84212413 | None:intergenic | 50.0% | |
| ATTGCTTGAGCTGACGCTTG+TGG | - | chr3.1:84212072-84212091 | MS.gene42655:CDS | 50.0% | |
| CATGCGAAAAACACTACCGC+CGG | - | chr3.1:84210777-84210796 | MS.gene42655:CDS | 50.0% | |
| GAATCAAGCATCCCAACGAG+AGG | + | chr3.1:84211185-84211204 | None:intergenic | 50.0% | |
| GGGCAATTTCTCCTCTCGTT+GGG | - | chr3.1:84211171-84211190 | MS.gene42655:CDS | 50.0% | |
| GTAGTGCCTTGGCACAGAAA+AGG | - | chr3.1:84212095-84212114 | MS.gene42655:CDS | 50.0% | |
| TTCTCCGCGTAACTCCTTCA+CGG | + | chr3.1:84210931-84210950 | None:intergenic | 50.0% | |
| TTGCTTGAGCTGACGCTTGT+GGG | - | chr3.1:84212073-84212092 | MS.gene42655:CDS | 50.0% | |
| ! | CAGGTTTCTGGGAGAAGTGA+TGG | - | chr3.1:84212160-84212179 | MS.gene42655:CDS | 50.0% |
| ! | CGGTAGTGTTTTTCGCATGG+TGG | + | chr3.1:84210776-84210795 | None:intergenic | 50.0% |
| ! | CTTCTTCGCGGTGGTAATGT+CGG | + | chr3.1:84210881-84210900 | None:intergenic | 50.0% |
| ! | GTTTCTGGGAGAAGTGATGG+TGG | - | chr3.1:84212163-84212182 | MS.gene42655:CDS | 50.0% |
| ! | TGATGCCCTTTTCTGTGCCA+AGG | + | chr3.1:84212104-84212123 | None:intergenic | 50.0% |
| !! | AATTAGCTCTGTCGCGTCGA+CGG | - | chr3.1:84210902-84210921 | MS.gene42655:CDS | 50.0% |
| !! | AATTTGGTGTCGAGTCCCGA+GGG | - | chr3.1:84211881-84211900 | MS.gene42655:intron | 50.0% |
| !! | TAGGAAGGGTATTGCACCAG+AGG | + | chr3.1:84211666-84211685 | None:intergenic | 50.0% |
| !! | TGGCACAGAAAAGGGCATCA+AGG | - | chr3.1:84212104-84212123 | MS.gene42655:CDS | 50.0% |
| AATGATGCGGCGGAAGGATG+AGG | + | chr3.1:84210820-84210839 | None:intergenic | 55.0% | |
| GCAACCGTGAAGGAGTTACG+CGG | - | chr3.1:84210924-84210943 | MS.gene42655:CDS | 55.0% | |
| GGGGCAATTTCTCCTCTCGT+TGG | - | chr3.1:84211170-84211189 | MS.gene42655:CDS | 55.0% | |
| GTCGAAAATGATGCGGCGGA+AGG | + | chr3.1:84210826-84210845 | None:intergenic | 55.0% | |
| ! | AGAGGCACACTCGCAATGCT+CGG | - | chr3.1:84211149-84211168 | MS.gene42655:CDS | 55.0% |
| ! | CGGCGGTAGTGTTTTTCGCA+TGG | + | chr3.1:84210779-84210798 | None:intergenic | 55.0% |
| ! | GTAATGTCGGTGGTGTCGAG+AGG | + | chr3.1:84210868-84210887 | None:intergenic | 55.0% |
| !! | AGGCGTCGAAAATGATGCGG+CGG | + | chr3.1:84210830-84210849 | None:intergenic | 55.0% |
| !! | CAATTTGGTGTCGAGTCCCG+AGG | - | chr3.1:84211880-84211899 | MS.gene42655:intron | 55.0% |
| AGACTCGACGAAGCGGCGAA+TGG | - | chr3.1:84211082-84211101 | MS.gene42655:CDS | 60.0% | |
| AGGCACACTCGCAATGCTCG+GGG | - | chr3.1:84211151-84211170 | MS.gene42655:CDS | 60.0% | |
| GACGCTTGTGGGTAGTGCCT+TGG | - | chr3.1:84212084-84212103 | MS.gene42655:CDS | 60.0% | |
| GAGGCACACTCGCAATGCTC+GGG | - | chr3.1:84211150-84211169 | MS.gene42655:CDS | 60.0% | |
| GCGGAGAAGACTCGACGAAG+CGG | - | chr3.1:84211075-84211094 | MS.gene42655:CDS | 60.0% | |
| GGAAGGATGAGGCGGAGAAG+CGG | + | chr3.1:84210809-84210828 | None:intergenic | 60.0% | |
| GGAGAAGCGGAAGTTACCGG+CGG | + | chr3.1:84210796-84210815 | None:intergenic | 60.0% | |
| GGCGGAGAAGCGGAAGTTAC+CGG | + | chr3.1:84210799-84210818 | None:intergenic | 60.0% | |
| TGCGAGTGTGCCTCTCGCTT+CGG | + | chr3.1:84211144-84211163 | None:intergenic | 60.0% | |
| TTCGACGCCTTAAGCTGCGG+CGG | - | chr3.1:84210840-84210859 | MS.gene42655:CDS | 60.0% | |
| ! | CTTCGCGGTGGTAATGTCGG+TGG | + | chr3.1:84210878-84210897 | None:intergenic | 60.0% |
| AGAGGCTCCGCCGCAGCTTA+AGG | + | chr3.1:84210850-84210869 | None:intergenic | 65.0% | |
| GACGAAGCGGCGAATGGCAG+CGG | - | chr3.1:84211088-84211107 | MS.gene42655:CDS | 65.0% | |
| GATGCGGCGGAAGGATGAGG+CGG | + | chr3.1:84210817-84210836 | None:intergenic | 65.0% | |
| ! | CGCGTCGACGGCAACCGTGA+AGG | - | chr3.1:84210914-84210933 | MS.gene42655:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 84210723 | 84212450 | 84210723 | ID=MS.gene42655 |
| chr3.1 | mRNA | 84210723 | 84212450 | 84210723 | ID=MS.gene42655.t1;Parent=MS.gene42655 |
| chr3.1 | exon | 84211903 | 84212450 | 84211903 | ID=MS.gene42655.t1.exon1;Parent=MS.gene42655.t1 |
| chr3.1 | CDS | 84211903 | 84212450 | 84211903 | ID=cds.MS.gene42655.t1;Parent=MS.gene42655.t1 |
| chr3.1 | exon | 84210723 | 84211662 | 84210723 | ID=MS.gene42655.t1.exon2;Parent=MS.gene42655.t1 |
| chr3.1 | CDS | 84210723 | 84211662 | 84210723 | ID=cds.MS.gene42655.t1;Parent=MS.gene42655.t1 |
| Gene Sequence |
| Protein sequence |
