AlfalfaGEDB | MS.gene43052

M. sativa cultivar XinJiangDaYe / MS.gene43052

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43052.t1	XP_024632109.1	90	150	15	0	1	150	5	154	5.40E-71	276.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43052.t1	Q9ZNU9	49.1	163	70	1	1	150	1	163	7.9e-39	161.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43052.t1	G7ITQ3	90.0	150	15	0	1	150	1	150	3.9e-71	276.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051343	MS.gene43052	0.807055	6.09E-50	-1.69E-46
MS.gene056699	MS.gene43052	0.801113	1.05E-48	-1.69E-46
MS.gene058592	MS.gene43052	0.817704	2.87E-52	-1.69E-46
MS.gene060244	MS.gene43052	0.820029	8.51E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43052.t1	MTR_2g086190	90.000	150	15	0	1	150	1	150	5.42e-98	278
MS.gene43052.t1	MTR_4g053440	57.059	170	53	3	1	150	1	170	1.02e-63	192
MS.gene43052.t1	MTR_7g104320	50.595	168	65	4	1	150	1	168	1.61e-51	162
MS.gene43052.t1	MTR_1g060380	48.077	156	75	2	1	150	1	156	8.24e-48	152
MS.gene43052.t1	MTR_2g098160	44.375	160	72	3	8	150	13	172	3.13e-44	143
MS.gene43052.t1	MTR_0249s0070	40.764	157	79	3	8	150	13	169	1.51e-39	131
MS.gene43052.t1	MTR_0249s0070	40.764	157	79	3	8	150	21	177	1.66e-39	131
MS.gene43052.t1	MTR_7g092400	42.236	161	74	4	8	150	101	260	1.99e-38	131
MS.gene43052.t1	MTR_4g065570	41.912	136	67	4	27	150	2	137	2.45e-32	112
MS.gene43052.t1	MTR_1g100773	37.333	150	71	3	9	148	2	138	3.86e-31	109
MS.gene43052.t1	MTR_7g114920	38.732	142	75	4	8	148	1	131	4.38e-31	108
MS.gene43052.t1	MTR_3g028010	35.417	144	80	2	12	150	28	163	2.05e-24	92.8
MS.gene43052.t1	MTR_2g054650	37.762	143	70	5	7	148	1	125	2.32e-24	91.7
MS.gene43052.t1	MTR_8g036980	44.792	96	51	2	55	150	58	151	2.09e-22	87.0
MS.gene43052.t1	MTR_3g025570	57.500	40	17	0	89	128	8	47	7.43e-13	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43052.t1	AT2G27580	48.571	175	53	2	1	150	1	163	2.82e-53	166
MS.gene43052.t1	AT2G27580	48.571	175	53	2	1	150	1	163	2.82e-53	166
MS.gene43052.t1	AT2G36320	49.068	161	71	3	1	150	1	161	1.28e-48	154
MS.gene43052.t1	AT3G52800	47.647	170	69	4	1	150	1	170	7.54e-44	142
MS.gene43052.t1	AT3G52800	47.647	170	69	4	1	150	1	170	7.54e-44	142
MS.gene43052.t1	AT1G12440	44.444	153	73	1	10	150	16	168	7.90e-43	139
MS.gene43052.t1	AT1G12440	44.444	153	73	1	10	150	16	168	7.90e-43	139
MS.gene43052.t1	AT1G12440	44.444	153	73	1	10	150	16	168	7.90e-43	139
MS.gene43052.t1	AT4G22820	42.405	158	74	3	10	150	19	176	7.92e-42	137
MS.gene43052.t1	AT4G22820	42.405	158	74	3	10	150	19	176	7.92e-42	137
MS.gene43052.t1	AT4G12040	43.558	163	67	5	10	150	16	175	5.61e-40	132
MS.gene43052.t1	AT4G12040	43.558	163	67	5	10	150	16	175	5.61e-40	132
MS.gene43052.t1	AT1G51200	42.683	164	70	4	8	150	13	173	5.10e-38	127
MS.gene43052.t1	AT1G51200	42.683	164	70	4	8	150	13	173	5.10e-38	127
MS.gene43052.t1	AT1G51200	42.683	164	70	4	8	150	13	173	5.10e-38	127
MS.gene43052.t1	AT1G51200	42.683	164	70	4	8	150	13	173	5.10e-38	127
MS.gene43052.t1	AT4G25380	36.691	139	69	3	13	150	10	130	3.10e-28	101
MS.gene43052.t1	AT4G14225	36.170	141	70	3	10	150	5	125	4.90e-27	98.2
MS.gene43052.t1	AT3G12630	37.037	135	78	2	18	150	31	160	6.85e-25	94.0

Find 50 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCACACGCATGCTGCTC+CGG	0.350425	2.4:+13934882	None:intergenic
GTCAACAATTGCGGTTTCTT+CGG	0.368301	2.4:-13935194	MS.gene43052:CDS
GTCATCTTCGTTGCCGGATC+CGG	0.388359	2.4:-13935036	MS.gene43052:CDS
ACCGGCGAGCGTTGCGGTGG+AGG	0.410293	2.4:-13935009	MS.gene43052:CDS
GGATTGATTGAGAACGAGTT+TGG	0.421778	2.4:+13935097	None:intergenic
AAGAAGCGCGTGGGGCTGAC+GGG	0.422797	2.4:-13934951	MS.gene43052:CDS
CTCGCCGGTCCAACCGGATC+CGG	0.425676	2.4:+13935023	None:intergenic
AGCTTCTCCCCTTTCACCAC+CGG	0.426041	2.4:+13934816	None:intergenic
TGAACCGTCATCTTCGTTGC+CGG	0.426897	2.4:-13935042	MS.gene43052:CDS
TCCTCCACCGCAACGCTCGC+CGG	0.433839	2.4:+13935008	None:intergenic
GCAACGCTCGCCGGTCCAAC+CGG	0.447445	2.4:+13935017	None:intergenic
ATCCATCGCAGCGTCTTCTC+CGG	0.463615	2.4:-13935078	MS.gene43052:CDS
GCAGATTGAGAAGGCGAATC+CGG	0.467485	2.4:-13934835	MS.gene43052:CDS
AGGCGAATCCGGTGGTGAAA+GGG	0.476689	2.4:-13934824	MS.gene43052:CDS
GGTGGTGAAAGGGGAGAAGC+TGG	0.489058	2.4:-13934814	MS.gene43052:CDS
CGCCGGAGAAGACGCTGCGA+TGG	0.504990	2.4:+13935076	None:intergenic
TAAGAAGCGCGTGGGGCTGA+CGG	0.505822	2.4:-13934952	MS.gene43052:CDS
GTTGCCGGATCCGGTTGGAC+CGG	0.505830	2.4:-13935027	MS.gene43052:CDS
CGCAGCGTCTTCTCCGGCGG+TGG	0.510882	2.4:-13935072	MS.gene43052:CDS
ACTCACACGCATGCTGCTCC+GGG	0.520253	2.4:+13934883	None:intergenic
AGAAACCGTTGCAACAGAAT+CGG	0.521190	2.4:-13934986	MS.gene43052:CDS
ACGCATGCTGCTCCGGGTAA+CGG	0.528429	2.4:+13934889	None:intergenic
AAGGCGAATCCGGTGGTGAA+AGG	0.529681	2.4:-13934825	MS.gene43052:CDS
CGATTATGCGTCAACAATTG+CGG	0.530355	2.4:-13935203	MS.gene43052:CDS
ATGTGGGACCCACCGTTACC+CGG	0.536651	2.4:-13934901	MS.gene43052:CDS
TCTTCGTTGCCGGATCCGGT+TGG	0.549850	2.4:-13935032	MS.gene43052:CDS
CATGCTGCTCCGGGTAACGG+TGG	0.563808	2.4:+13934892	None:intergenic
TGGACCGGCGAGCGTTGCGG+TGG	0.567623	2.4:-13935012	MS.gene43052:CDS
GGCGAATCCGGTGGTGAAAG+GGG	0.569534	2.4:-13934823	MS.gene43052:CDS
AGAAGCGCGTGGGGCTGACG+GGG	0.576489	2.4:-13934950	MS.gene43052:CDS
ACGGGGTTTAAGTGCAAGTG+TGG	0.578699	2.4:-13934933	MS.gene43052:CDS
TTTCTTCGGCAACGCCGCAA+CGG	0.579159	2.4:-13935180	MS.gene43052:CDS
ATACACCGATTCTGTTGCAA+CGG	0.585507	2.4:+13934981	None:intergenic
GGTTGGACCGGCGAGCGTTG+CGG	0.586676	2.4:-13935015	MS.gene43052:CDS
CATCGCAGCGTCTTCTCCGG+CGG	0.604757	2.4:-13935075	MS.gene43052:CDS
CAATTGTTGACGCATAATCG+TGG	0.606724	2.4:+13935206	None:intergenic
ATGCTGCTCCGGGTAACGGT+GGG	0.609178	2.4:+13934893	None:intergenic
ATGACATGTAAGAAGCGCGT+GGG	0.615643	2.4:-13934960	MS.gene43052:CDS
GGTTTAAGTGCAAGTGTGGG+AGG	0.629260	2.4:-13934929	MS.gene43052:CDS
GGGGAAAGAGCAGATTGAGA+AGG	0.632174	2.4:-13934844	MS.gene43052:CDS
AAGTGTGGGAGGATGTTATG+TGG	0.638134	2.4:-13934918	MS.gene43052:CDS
CGGGGTTTAAGTGCAAGTGT+GGG	0.646854	2.4:-13934932	MS.gene43052:CDS
AGTGTGGGAGGATGTTATGT+GGG	0.654340	2.4:-13934917	MS.gene43052:CDS
GGTTCAGAAACAACCACCGC+CGG	0.669731	2.4:+13935059	None:intergenic
TATGACATGTAAGAAGCGCG+TGG	0.671005	2.4:-13934961	MS.gene43052:CDS
CTTTGAGCTGTAAATCACGA+TGG	0.672254	2.4:+13935138	None:intergenic
GATTGAGAAGGCGAATCCGG+TGG	0.672661	2.4:-13934832	MS.gene43052:CDS
TGAACAGAGATTCTCCGTTG+CGG	0.674548	2.4:+13935166	None:intergenic
GGATCCGGCAACGAAGATGA+CGG	0.681329	2.4:+13935038	None:intergenic
TGACATGTAAGAAGCGCGTG+GGG	0.730702	2.4:-13934959	MS.gene43052:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTTGATTTTAAGGGGTTG+GGG	-	chr2.4:13935173-13935192	MS.gene43052:CDS	35.0%
!!	CGTGTGAGTTTGATTTTAAG+GGG	-	chr2.4:13935167-13935186	MS.gene43052:CDS	35.0%
!!	GAGTTTGATTTTAAGGGGTT+GGG	-	chr2.4:13935172-13935191	MS.gene43052:CDS	35.0%
!!	GCGTGTGAGTTTGATTTTAA+GGG	-	chr2.4:13935166-13935185	MS.gene43052:CDS	35.0%
!!	TGAGTTTGATTTTAAGGGGT+TGG	-	chr2.4:13935171-13935190	MS.gene43052:CDS	35.0%
!!	TGCGTGTGAGTTTGATTTTA+AGG	-	chr2.4:13935165-13935184	MS.gene43052:CDS	35.0%
	AGAAACCGTTGCAACAGAAT+CGG	-	chr2.4:13935050-13935069	MS.gene43052:CDS	40.0%
	CAATTGTTGACGCATAATCG+TGG	+	chr2.4:13934833-13934852	None:intergenic	40.0%
	CGATTATGCGTCAACAATTG+CGG	-	chr2.4:13934833-13934852	MS.gene43052:CDS	40.0%
	CTTTGAGCTGTAAATCACGA+TGG	+	chr2.4:13934901-13934920	None:intergenic	40.0%
	GGATTGATTGAGAACGAGTT+TGG	+	chr2.4:13934942-13934961	None:intergenic	40.0%
	GTCAACAATTGCGGTTTCTT+CGG	-	chr2.4:13934842-13934861	MS.gene43052:CDS	40.0%
!!	ATACACCGATTCTGTTGCAA+CGG	+	chr2.4:13935058-13935077	None:intergenic	40.0%
	AAGTGTGGGAGGATGTTATG+TGG	-	chr2.4:13935118-13935137	MS.gene43052:CDS	45.0%
	AGTGTGGGAGGATGTTATGT+GGG	-	chr2.4:13935119-13935138	MS.gene43052:CDS	45.0%
	TGAACAGAGATTCTCCGTTG+CGG	+	chr2.4:13934873-13934892	None:intergenic	45.0%
!	ATGACATGTAAGAAGCGCGT+GGG	-	chr2.4:13935076-13935095	MS.gene43052:CDS	45.0%
!	TATGACATGTAAGAAGCGCG+TGG	-	chr2.4:13935075-13935094	MS.gene43052:CDS	45.0%
	ACGGGGTTTAAGTGCAAGTG+TGG	-	chr2.4:13935103-13935122	MS.gene43052:CDS	50.0%
	CGGGGTTTAAGTGCAAGTGT+GGG	-	chr2.4:13935104-13935123	MS.gene43052:CDS	50.0%
	GCAGATTGAGAAGGCGAATC+CGG	-	chr2.4:13935201-13935220	MS.gene43052:CDS	50.0%
	GGGGAAAGAGCAGATTGAGA+AGG	-	chr2.4:13935192-13935211	MS.gene43052:CDS	50.0%
	GGTTTAAGTGCAAGTGTGGG+AGG	-	chr2.4:13935107-13935126	MS.gene43052:CDS	50.0%
	TGAACCGTCATCTTCGTTGC+CGG	-	chr2.4:13934994-13935013	MS.gene43052:CDS	50.0%
!	TGACATGTAAGAAGCGCGTG+GGG	-	chr2.4:13935077-13935096	MS.gene43052:CDS	50.0%
	AACTCACACGCATGCTGCTC+CGG	+	chr2.4:13935157-13935176	None:intergenic	55.0%
	AAGGCGAATCCGGTGGTGAA+AGG	-	chr2.4:13935211-13935230	MS.gene43052:CDS	55.0%
	AGCTTCTCCCCTTTCACCAC+CGG	+	chr2.4:13935223-13935242	None:intergenic	55.0%
	AGGCGAATCCGGTGGTGAAA+GGG	-	chr2.4:13935212-13935231	MS.gene43052:CDS	55.0%
	ATCCATCGCAGCGTCTTCTC+CGG	-	chr2.4:13934958-13934977	MS.gene43052:CDS	55.0%
	GATTGAGAAGGCGAATCCGG+TGG	-	chr2.4:13935204-13935223	MS.gene43052:CDS	55.0%
	GGATCCGGCAACGAAGATGA+CGG	+	chr2.4:13935001-13935020	None:intergenic	55.0%
	GGTTCAGAAACAACCACCGC+CGG	+	chr2.4:13934980-13934999	None:intergenic	55.0%
	GTCATCTTCGTTGCCGGATC+CGG	-	chr2.4:13935000-13935019	MS.gene43052:CDS	55.0%
	TTTCTTCGGCAACGCCGCAA+CGG	-	chr2.4:13934856-13934875	MS.gene43052:CDS	55.0%
	ACGCATGCTGCTCCGGGTAA+CGG	+	chr2.4:13935150-13935169	None:intergenic	60.0%
	ACTCACACGCATGCTGCTCC+GGG	+	chr2.4:13935156-13935175	None:intergenic	60.0%
	ATGTGGGACCCACCGTTACC+CGG	-	chr2.4:13935135-13935154	MS.gene43052:CDS	60.0%
	GGCGAATCCGGTGGTGAAAG+GGG	-	chr2.4:13935213-13935232	MS.gene43052:CDS	60.0%
	GGTGGTGAAAGGGGAGAAGC+TGG	-	chr2.4:13935222-13935241	MS.gene43052:CDS	60.0%
	TCTTCGTTGCCGGATCCGGT+TGG	-	chr2.4:13935004-13935023	MS.gene43052:CDS	60.0%
!	ATGCTGCTCCGGGTAACGGT+GGG	+	chr2.4:13935146-13935165	None:intergenic	60.0%
!	TAAGAAGCGCGTGGGGCTGA+CGG	-	chr2.4:13935084-13935103	MS.gene43052:CDS	60.0%
	CATCGCAGCGTCTTCTCCGG+CGG	-	chr2.4:13934961-13934980	MS.gene43052:CDS	65.0%
	GTTGCCGGATCCGGTTGGAC+CGG	-	chr2.4:13935009-13935028	MS.gene43052:CDS	65.0%
!	AAGAAGCGCGTGGGGCTGAC+GGG	-	chr2.4:13935085-13935104	MS.gene43052:CDS	65.0%
!	CATGCTGCTCCGGGTAACGG+TGG	+	chr2.4:13935147-13935166	None:intergenic	65.0%
	CGCCGGAGAAGACGCTGCGA+TGG	+	chr2.4:13934963-13934982	None:intergenic	70.0%
	CTCGCCGGTCCAACCGGATC+CGG	+	chr2.4:13935016-13935035	None:intergenic	70.0%
	GCAACGCTCGCCGGTCCAAC+CGG	+	chr2.4:13935022-13935041	None:intergenic	70.0%
	TCCTCCACCGCAACGCTCGC+CGG	+	chr2.4:13935031-13935050	None:intergenic	70.0%
!	AGAAGCGCGTGGGGCTGACG+GGG	-	chr2.4:13935086-13935105	MS.gene43052:CDS	70.0%
!	GGTTGGACCGGCGAGCGTTG+CGG	-	chr2.4:13935021-13935040	MS.gene43052:CDS	70.0%
	CGCAGCGTCTTCTCCGGCGG+TGG	-	chr2.4:13934964-13934983	MS.gene43052:CDS	75.0%
!	ACCGGCGAGCGTTGCGGTGG+AGG	-	chr2.4:13935027-13935046	MS.gene43052:CDS	75.0%
!	TGGACCGGCGAGCGTTGCGG+TGG	-	chr2.4:13935024-13935043	MS.gene43052:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	13934803	13935255	13934803	ID=MS.gene43052
chr2.4	mRNA	13934803	13935255	13934803	ID=MS.gene43052.t1;Parent=MS.gene43052
chr2.4	exon	13934803	13935255	13934803	ID=MS.gene43052.t1.exon1;Parent=MS.gene43052.t1
chr2.4	CDS	13934803	13935255	13934803	ID=cds.MS.gene43052.t1;Parent=MS.gene43052.t1

Gene Sequence

>MS.gene43052

ATGGCGGAAGAACACAGATTGCAAGCACCACGATTATGCGTCAACAATTGCGGTTTCTTCGGCAACGCCGCAACGGAGAATCTCTGTTCAAGATGCCATCGTGATTTACAGCTCAAAGAACAACAAGCTTCATCAGCCAAACTCGTTCTCAATCAATCCATCGCAGCGTCTTCTCCGGCGGTGGTTGTTTCTGAACCGTCATCTTCGTTGCCGGATCCGGTTGGACCGGCGAGCGTTGCGGTGGAGGAGAAACCGTTGCAACAGAATCGGTGTATGACATGTAAGAAGCGCGTGGGGCTGACGGGGTTTAAGTGCAAGTGTGGGAGGATGTTATGTGGGACCCACCGTTACCCGGAGCAGCATGCGTGTGAGTTTGATTTTAAGGGGTTGGGGAAAGAGCAGATTGAGAAGGCGAATCCGGTGGTGAAAGGGGAGAAGCTGGAGAAGATCTGA

Protein sequence

>MS.gene43052.t1

MAEEHRLQAPRLCVNNCGFFGNAATENLCSRCHRDLQLKEQQASSAKLVLNQSIAASSPAVVVSEPSSSLPDPVGPASVAVEEKPLQQNRCMTCKKRVGLTGFKCKCGRMLCGTHRYPEQHACEFDFKGLGKEQIEKANPVVKGEKLEKI