Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43077.t1 | XP_013464923.1 | 99.1 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 5.40E-52 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43077.t1 | Q9FK81 | 65.7 | 99 | 34 | 0 | 7 | 105 | 6 | 104 | 1.7e-30 | 133.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43077.t1 | B7FMZ0 | 99.1 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 3.9e-52 | 213.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056542 | MS.gene43077 | 0.805507 | 1.29E-49 | -1.69E-46 |
| MS.gene056543 | MS.gene43077 | 0.807195 | 5.69E-50 | -1.69E-46 |
| MS.gene056545 | MS.gene43077 | 0.809325 | 2.00E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43077 | MS.gene012190 | PPI |
| MS.gene43077 | MS.gene24782 | PPI |
| MS.gene25266 | MS.gene43077 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43077.t1 | MTR_2g084985 | 99.099 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 6.00e-75 | 217 |
| MS.gene43077.t1 | MTR_4g124920 | 41.584 | 101 | 55 | 2 | 8 | 105 | 4 | 103 | 1.40e-21 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43077.t1 | AT5G22580 | 65.657 | 99 | 34 | 0 | 7 | 105 | 6 | 104 | 5.30e-42 | 134 |
| MS.gene43077.t1 | AT3G17210 | 42.000 | 100 | 54 | 2 | 8 | 104 | 9 | 107 | 3.39e-22 | 84.0 |
| MS.gene43077.t1 | AT3G17210 | 42.000 | 100 | 54 | 2 | 8 | 104 | 9 | 107 | 3.39e-22 | 84.0 |
Find 26 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATGCTGCTTCCTTAAC+TGG | 0.195704 | 2.4:-14265642 | None:intergenic |
| TACAAAAGGGATGGAGAAAT+TGG | 0.322091 | 2.4:+14264804 | MS.gene43077:CDS |
| ATCTTGTCAAATCCTTTGAA+TGG | 0.422994 | 2.4:+14264839 | MS.gene43077:CDS |
| AACTGGCTTGACAAGGGTTG+AGG | 0.423667 | 2.4:-14265625 | None:intergenic |
| AACCCTTGTCAAGCCAGTTA+AGG | 0.431460 | 2.4:+14265629 | MS.gene43077:CDS |
| CTTCCTTAACTGGCTTGACA+AGG | 0.454173 | 2.4:-14265632 | None:intergenic |
| CTTGTTGAATGTCATCAAGA+AGG | 0.457412 | 2.4:-14265505 | None:intergenic |
| GTGATTGTTAAGTTCAAAGA+AGG | 0.458164 | 2.4:+14264763 | MS.gene43077:CDS |
| GTAGTGGAAGAGCTTACAAA+AGG | 0.480357 | 2.4:+14264790 | MS.gene43077:CDS |
| GTTCAAAGAAGGTGTAGTAG+TGG | 0.481023 | 2.4:+14264774 | MS.gene43077:CDS |
| GGGACAGGATATTGAAAGCC+TGG | 0.493701 | 2.4:+14265455 | MS.gene43077:CDS |
| TCAGAGAACTCAACATGACT+TGG | 0.494285 | 2.4:-14265558 | None:intergenic |
| AACCTTGTCTCAACACATCC+AGG | 0.501843 | 2.4:-14265473 | None:intergenic |
| ACTGGCTTGACAAGGGTTGA+GGG | 0.505995 | 2.4:-14265624 | None:intergenic |
| AGAACTCAACATGACTTGGA+TGG | 0.554383 | 2.4:-14265554 | None:intergenic |
| GTATGTCTTATACAGGGGAC+AGG | 0.555403 | 2.4:+14265440 | MS.gene43077:intron |
| TTCACAAAGAATGGTAGTCA+TGG | 0.559784 | 2.4:+14264723 | None:intergenic |
| TTCCTTAACTGGCTTGACAA+GGG | 0.564730 | 2.4:-14265631 | None:intergenic |
| CTGGCTTGACAAGGGTTGAG+GGG | 0.581401 | 2.4:-14265623 | None:intergenic |
| GTATAAAACATACCATTCAA+AGG | 0.584418 | 2.4:-14264851 | None:intergenic |
| TAGTGGAAGAGCTTACAAAA+GGG | 0.588758 | 2.4:+14264791 | MS.gene43077:CDS |
| GGAAGAGCTTACAAAAGGGA+TGG | 0.589362 | 2.4:+14264795 | MS.gene43077:CDS |
| TGAATGTCATCAAGAAGGCA+TGG | 0.625633 | 2.4:-14265500 | None:intergenic |
| AGCCTGGATGTGTTGAGACA+AGG | 0.635747 | 2.4:+14265471 | MS.gene43077:CDS |
| ACAAAGAATGGTAGTCATGG+AGG | 0.636423 | 2.4:+14264726 | None:intergenic |
| GAATGTCATCAAGAAGGCAT+GGG | 0.659643 | 2.4:-14265499 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATCAAAAAATTTGATTTT+GGG | + | chr2.4:14265171-14265190 | MS.gene43077:intron | 15.0% |
| !! | AACAAAGACAGTAATTTAAA+GGG | + | chr2.4:14265051-14265070 | MS.gene43077:intron | 20.0% |
| !!! | GACATCAAAAAATTTGATTT+TGG | + | chr2.4:14265170-14265189 | MS.gene43077:intron | 20.0% |
| ! | AAAAACTATCAGTATCTATG+AGG | - | chr2.4:14264969-14264988 | None:intergenic | 25.0% |
| ! | AACAGAAACAGAAATAACAA+GGG | + | chr2.4:14265018-14265037 | MS.gene43077:intron | 25.0% |
| ! | AATCAAGTATGTCTTATACA+GGG | + | chr2.4:14265434-14265453 | MS.gene43077:intron | 25.0% |
| ! | ACTGAGATTTATAGCTAAAT+AGG | + | chr2.4:14265117-14265136 | MS.gene43077:intron | 25.0% |
| ! | ATGATATGATAGTACTAAGT+AGG | - | chr2.4:14265405-14265424 | None:intergenic | 25.0% |
| ! | ATGTGACGATTATGATTATT+TGG | + | chr2.4:14265353-14265372 | MS.gene43077:intron | 25.0% |
| ! | GAACAAAGACAGTAATTTAA+AGG | + | chr2.4:14265050-14265069 | MS.gene43077:intron | 25.0% |
| ! | GTATAAAACATACCATTCAA+AGG | - | chr2.4:14264854-14264873 | None:intergenic | 25.0% |
| ! | TAACAGAAACAGAAATAACA+AGG | + | chr2.4:14265017-14265036 | MS.gene43077:intron | 25.0% |
| ! | TAATCAAGTATGTCTTATAC+AGG | + | chr2.4:14265433-14265452 | MS.gene43077:intron | 25.0% |
| ! | TGATATGATAGTACTAAGTA+GGG | - | chr2.4:14265404-14265423 | None:intergenic | 25.0% |
| !! | TACCTTTTATATTATGCAAG+AGG | + | chr2.4:14264877-14264896 | MS.gene43077:intron | 25.0% |
| ATCAAGTATGTCTTATACAG+GGG | + | chr2.4:14265435-14265454 | MS.gene43077:intron | 30.0% | |
| ATCTTGTCAAATCCTTTGAA+TGG | + | chr2.4:14264839-14264858 | MS.gene43077:CDS | 30.0% | |
| CTATGAGGTTAATAAACTGA+AGG | - | chr2.4:14264954-14264973 | None:intergenic | 30.0% | |
| GTGATTGTTAAGTTCAAAGA+AGG | + | chr2.4:14264763-14264782 | MS.gene43077:CDS | 30.0% | |
| TGCCTCTTGCATAATATAAA+AGG | - | chr2.4:14264882-14264901 | None:intergenic | 30.0% | |
| CTTGTTGAATGTCATCAAGA+AGG | - | chr2.4:14265508-14265527 | None:intergenic | 35.0% | |
| TACAAAAGGGATGGAGAAAT+TGG | + | chr2.4:14264804-14264823 | MS.gene43077:CDS | 35.0% | |
| TAGTGGAAGAGCTTACAAAA+GGG | + | chr2.4:14264791-14264810 | MS.gene43077:CDS | 35.0% | |
| ! | GTATACGATTAGTTGTGACT+TGG | - | chr2.4:14265289-14265308 | None:intergenic | 35.0% |
| ! | TTGATTTTGGGCTTACATCA+CGG | + | chr2.4:14265183-14265202 | MS.gene43077:intron | 35.0% |
| !!! | ATTTTTTGATGTCTTCGCGT+TGG | - | chr2.4:14265163-14265182 | None:intergenic | 35.0% |
| TCAGAGAACTCAACATGACT+TGG | - | chr2.4:14265561-14265580 | None:intergenic | 40.0% | |
| TGAATGTCATCAAGAAGGCA+TGG | - | chr2.4:14265503-14265522 | None:intergenic | 40.0% | |
| ! | AGAACTCAACATGACTTGGA+TGG | - | chr2.4:14265557-14265576 | None:intergenic | 40.0% |
| ! | GAATGTCATCAAGAAGGCAT+GGG | - | chr2.4:14265502-14265521 | None:intergenic | 40.0% |
| ! | GTAGTGGAAGAGCTTACAAA+AGG | + | chr2.4:14264790-14264809 | MS.gene43077:CDS | 40.0% |
| ! | GTTCAAAGAAGGTGTAGTAG+TGG | + | chr2.4:14264774-14264793 | MS.gene43077:CDS | 40.0% |
| !! | GATTAGTTGTGACTTGGTGT+CGG | - | chr2.4:14265283-14265302 | None:intergenic | 40.0% |
| !! | TTCCTTAACTGGCTTGACAA+GGG | - | chr2.4:14265634-14265653 | None:intergenic | 40.0% |
| AACCCTTGTCAAGCCAGTTA+AGG | + | chr2.4:14265629-14265648 | MS.gene43077:CDS | 45.0% | |
| AACCTTGTCTCAACACATCC+AGG | - | chr2.4:14265476-14265495 | None:intergenic | 45.0% | |
| GGAAGAGCTTACAAAAGGGA+TGG | + | chr2.4:14264795-14264814 | MS.gene43077:CDS | 45.0% | |
| GTATGTCTTATACAGGGGAC+AGG | + | chr2.4:14265440-14265459 | MS.gene43077:intron | 45.0% | |
| !! | AGCTAAATAGGCGTCGATTG+CGG | + | chr2.4:14265129-14265148 | MS.gene43077:intron | 45.0% |
| !! | ATGTCTTCGCGTTGGTAATG+TGG | - | chr2.4:14265155-14265174 | None:intergenic | 45.0% |
| !! | CTTCCTTAACTGGCTTGACA+AGG | - | chr2.4:14265635-14265654 | None:intergenic | 45.0% |
| AAGGGAAATTCCCCAACCAG+AGG | + | chr2.4:14265069-14265088 | MS.gene43077:intron | 50.0% | |
| AATTCCCCAACCAGAGGTTG+CGG | + | chr2.4:14265075-14265094 | MS.gene43077:intron | 50.0% | |
| AGAGGTTGCGGTCATGTTCA+CGG | + | chr2.4:14265087-14265106 | MS.gene43077:intron | 50.0% | |
| AGCCTGGATGTGTTGAGACA+AGG | + | chr2.4:14265471-14265490 | MS.gene43077:CDS | 50.0% | |
| CGGTCGTAGACTCGTAGTTA+TGG | + | chr2.4:14265203-14265222 | MS.gene43077:intron | 50.0% | |
| GGGACAGGATATTGAAAGCC+TGG | + | chr2.4:14265455-14265474 | MS.gene43077:CDS | 50.0% | |
| !! | AACTGGCTTGACAAGGGTTG+AGG | - | chr2.4:14265628-14265647 | None:intergenic | 50.0% |
| !! | ACTGGCTTGACAAGGGTTGA+GGG | - | chr2.4:14265627-14265646 | None:intergenic | 50.0% |
| ACATGACCGCAACCTCTGGT+TGG | - | chr2.4:14265084-14265103 | None:intergenic | 55.0% | |
| ATGACCGCAACCTCTGGTTG+GGG | - | chr2.4:14265082-14265101 | None:intergenic | 55.0% | |
| CATGACCGCAACCTCTGGTT+GGG | - | chr2.4:14265083-14265102 | None:intergenic | 55.0% | |
| GTGAACATGACCGCAACCTC+TGG | - | chr2.4:14265088-14265107 | None:intergenic | 55.0% | |
| ! | CTGGCTTGACAAGGGTTGAG+GGG | - | chr2.4:14265626-14265645 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 14264733 | 14265662 | 14264733 | ID=MS.gene43077 |
| chr2.4 | mRNA | 14264733 | 14265662 | 14264733 | ID=MS.gene43077.t1;Parent=MS.gene43077 |
| chr2.4 | exon | 14264733 | 14264860 | 14264733 | ID=MS.gene43077.t1.exon1;Parent=MS.gene43077.t1 |
| chr2.4 | CDS | 14264733 | 14264860 | 14264733 | ID=cds.MS.gene43077.t1;Parent=MS.gene43077.t1 |
| chr2.4 | exon | 14265455 | 14265662 | 14265455 | ID=MS.gene43077.t1.exon2;Parent=MS.gene43077.t1 |
| chr2.4 | CDS | 14265455 | 14265662 | 14265455 | ID=cds.MS.gene43077.t1;Parent=MS.gene43077.t1 |
| Gene Sequence |
| Protein sequence |
