Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24782.t1 | XP_003609973.1 | 96.1 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 3.60E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24782.t1 | Q9LUV2 | 71.6 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 5.6e-42 | 171.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24782.t1 | G7JS72 | 96.1 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 2.6e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene073085 | MS.gene24782 | PPI |
| MS.gene24782 | MS.gene90649 | PPI |
| MS.gene43077 | MS.gene24782 | PPI |
| MS.gene35373 | MS.gene24782 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24782.t1 | MTR_4g124920 | 96.117 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 6.88e-69 | 201 |
| MS.gene24782.t1 | MTR_4g124920 | 95.714 | 70 | 3 | 0 | 7 | 76 | 2 | 71 | 3.77e-44 | 139 |
| MS.gene24782.t1 | MTR_2g084985 | 39.216 | 102 | 58 | 2 | 8 | 108 | 7 | 105 | 5.52e-20 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24782.t1 | AT3G17210 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 2.86e-56 | 170 |
| MS.gene24782.t1 | AT3G17210 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 2.86e-56 | 170 |
| MS.gene24782.t1 | AT5G22580 | 39.000 | 100 | 57 | 2 | 4 | 102 | 2 | 98 | 1.44e-22 | 85.1 |
Find 22 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAGCCACATACTCTTTAA+TGG | 0.270013 | 8.2:-7931501 | None:intergenic |
| TACAATCACTTTCTCCAAGT+TGG | 0.390001 | 8.2:-7931558 | None:intergenic |
| AAATTTGTTCCTTTCCAACT+TGG | 0.431812 | 8.2:+7931544 | MS.gene24782:CDS |
| AAAGAAATGGCGGAAGCAAA+AGG | 0.444914 | 8.2:+7930473 | None:intergenic |
| CATAGTGATCGCAAAGTTCA+AGG | 0.471142 | 8.2:+7930508 | MS.gene24782:CDS |
| ATACCAGTGGAATGACTTCA+TGG | 0.474858 | 8.2:-7930600 | None:intergenic |
| GAAGATTGATGAACTCATCA+AGG | 0.501185 | 8.2:+7930547 | MS.gene24782:CDS |
| CATGAGTAAAACCTTGATGC+AGG | 0.509216 | 8.2:-7931448 | None:intergenic |
| TTTGCATACTCAACATGTGC+AGG | 0.522063 | 8.2:-7931524 | None:intergenic |
| CGCCCATGAAGTCATTCCAC+TGG | 0.526302 | 8.2:+7930597 | MS.gene24782:CDS |
| AATGGCTTCCACACTCTCAA+AGG | 0.541657 | 8.2:-7931483 | None:intergenic |
| AATCAAAGATTAACGATGGT+GGG | 0.542487 | 8.2:-7931593 | None:intergenic |
| TGAATCAGCCTTTGAGAGTG+TGG | 0.555458 | 8.2:+7931475 | MS.gene24782:CDS |
| AAATCAAAGATTAACGATGG+TGG | 0.556350 | 8.2:-7931594 | None:intergenic |
| TCACTTTCTCCAAGTTGGAA+AGG | 0.558231 | 8.2:-7931553 | None:intergenic |
| AGTGCTGAAAACCTGCATCA+AGG | 0.585611 | 8.2:+7931437 | MS.gene24782:CDS |
| GGAAGCCATTAAAGAGTATG+TGG | 0.589757 | 8.2:+7931496 | MS.gene24782:CDS |
| AGTGGAATGACTTCATGGGC+GGG | 0.607686 | 8.2:-7930595 | None:intergenic |
| CAGTGGAATGACTTCATGGG+CGG | 0.628089 | 8.2:-7930596 | None:intergenic |
| TACCAGTGGAATGACTTCAT+GGG | 0.633875 | 8.2:-7930599 | None:intergenic |
| AAGATTGATGAACTCATCAA+GGG | 0.636945 | 8.2:+7930548 | MS.gene24782:CDS |
| AATAAATCAAAGATTAACGA+TGG | 0.651109 | 8.2:-7931597 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAATTCATTTAATAATT+GGG | + | chr8.2:7930771-7930790 | MS.gene24782:intron | 10.0% |
| !! | TAGTAAATTCATTTAATAAT+TGG | + | chr8.2:7930770-7930789 | MS.gene24782:intron | 10.0% |
| !! | GTAAATTCATTTAATAATTG+GGG | + | chr8.2:7930772-7930791 | MS.gene24782:intron | 15.0% |
| !! | TAAAACTAATCTATTACATT+AGG | - | chr8.2:7931042-7931061 | None:intergenic | 15.0% |
| !! | TAATAGTACATAATGAATGA+AGG | - | chr8.2:7930633-7930652 | None:intergenic | 20.0% |
| !! | TATCTATTGTAAATATCACT+TGG | - | chr8.2:7931016-7931035 | None:intergenic | 20.0% |
| !!! | AAAATTAAGTCATACAGTTT+TGG | + | chr8.2:7931161-7931180 | MS.gene24782:intron | 20.0% |
| !!! | GTTATCTTTTATTGTGTATA+GGG | + | chr8.2:7931403-7931422 | MS.gene24782:intron | 20.0% |
| !!! | TGTTATCTTTTATTGTGTAT+AGG | + | chr8.2:7931402-7931421 | MS.gene24782:intron | 20.0% |
| !!! | TTATCTTTTATTGTGTATAG+GGG | + | chr8.2:7931404-7931423 | MS.gene24782:intron | 20.0% |
| ! | GTTGTTTACATAAGTGATTA+TGG | + | chr8.2:7930942-7930961 | MS.gene24782:intron | 25.0% |
| !! | TATCTTTTATTGTGTATAGG+GGG | + | chr8.2:7931405-7931424 | MS.gene24782:intron | 25.0% |
| AAGATTGATGAACTCATCAA+GGG | + | chr8.2:7930548-7930567 | MS.gene24782:CDS | 30.0% | |
| AGAATCAGTGGAACATATTT+GGG | + | chr8.2:7930814-7930833 | MS.gene24782:intron | 30.0% | |
| CAAATTGATGCTGCAAAAAA+TGG | + | chr8.2:7930721-7930740 | MS.gene24782:intron | 30.0% | |
| TAAACAACAGATGGCAAAAA+AGG | - | chr8.2:7930930-7930949 | None:intergenic | 30.0% | |
| TAGTACATAATGAATGAAGG+AGG | - | chr8.2:7930630-7930649 | None:intergenic | 30.0% | |
| TCACTTATGTAAACAACAGA+TGG | - | chr8.2:7930939-7930958 | None:intergenic | 30.0% | |
| TGATTCTCCTAATCTTACTT+GGG | - | chr8.2:7930801-7930820 | None:intergenic | 30.0% | |
| ! | AAATTTGTTCCTTTCCAACT+TGG | + | chr8.2:7931544-7931563 | MS.gene24782:CDS | 30.0% |
| ! | ACATTAGTCTCAAAAAGACT+AGG | + | chr8.2:7930979-7930998 | MS.gene24782:intron | 30.0% |
| ! | CATTAGTCTCAAAAAGACTA+GGG | + | chr8.2:7930980-7930999 | MS.gene24782:intron | 30.0% |
| AGTAAGATTAGGAGAATCAG+TGG | + | chr8.2:7930802-7930821 | MS.gene24782:intron | 35.0% | |
| ATGAGCCACATACTCTTTAA+TGG | - | chr8.2:7931504-7931523 | None:intergenic | 35.0% | |
| CAATCTAAACTGACCATTAG+TGG | + | chr8.2:7931330-7931349 | MS.gene24782:intron | 35.0% | |
| CTGATTCTCCTAATCTTACT+TGG | - | chr8.2:7930802-7930821 | None:intergenic | 35.0% | |
| GAAGATTGATGAACTCATCA+AGG | + | chr8.2:7930547-7930566 | MS.gene24782:CDS | 35.0% | |
| GAATCAGTGGAACATATTTG+GGG | + | chr8.2:7930815-7930834 | MS.gene24782:intron | 35.0% | |
| GAGAATCAGTGGAACATATT+TGG | + | chr8.2:7930813-7930832 | MS.gene24782:intron | 35.0% | |
| GTTGTAATCATAGCCACTAA+TGG | - | chr8.2:7931346-7931365 | None:intergenic | 35.0% | |
| TACAATCACTTTCTCCAAGT+TGG | - | chr8.2:7931561-7931580 | None:intergenic | 35.0% | |
| TCTCAAAAAGACTAGGGAAA+TGG | + | chr8.2:7930986-7931005 | MS.gene24782:intron | 35.0% | |
| ! | ATATTTGCGCTGATCTAAGT+GGG | - | chr8.2:7930680-7930699 | None:intergenic | 35.0% |
| ! | TTTTATTGTGTATAGGGGGA+CGG | + | chr8.2:7931409-7931428 | MS.gene24782:intron | 35.0% |
| !! | CTTATTTCTATAAGCTCTCC+AGG | - | chr8.2:7931217-7931236 | None:intergenic | 35.0% |
| AAGCGCTTATGAGACTGTTT+GGG | - | chr8.2:7931286-7931305 | None:intergenic | 40.0% | |
| ATACCAGTGGAATGACTTCA+TGG | - | chr8.2:7930603-7930622 | None:intergenic | 40.0% | |
| ATGAAGTCATTCCACTGGTA+TGG | + | chr8.2:7930602-7930621 | MS.gene24782:intron | 40.0% | |
| CATAGTGATCGCAAAGTTCA+AGG | + | chr8.2:7930508-7930527 | MS.gene24782:CDS | 40.0% | |
| CATGAGTAAAACCTTGATGC+AGG | - | chr8.2:7931451-7931470 | None:intergenic | 40.0% | |
| TAAGCGCTTATGAGACTGTT+TGG | - | chr8.2:7931287-7931306 | None:intergenic | 40.0% | |
| TACCAGTGGAATGACTTCAT+GGG | - | chr8.2:7930602-7930621 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGTGC+AGG | - | chr8.2:7931527-7931546 | None:intergenic | 40.0% | |
| ! | GATATTTGCGCTGATCTAAG+TGG | - | chr8.2:7930681-7930700 | None:intergenic | 40.0% |
| ! | GGAAGCCATTAAAGAGTATG+TGG | + | chr8.2:7931496-7931515 | MS.gene24782:CDS | 40.0% |
| ! | TCACTTTCTCCAAGTTGGAA+AGG | - | chr8.2:7931556-7931575 | None:intergenic | 40.0% |
| !!! | GCTGTTTTGATAAGCTATCC+TGG | + | chr8.2:7931196-7931215 | MS.gene24782:intron | 40.0% |
| AATGGCTTCCACACTCTCAA+AGG | - | chr8.2:7931486-7931505 | None:intergenic | 45.0% | |
| AGACTGTTTGGGAGAGCTTA+TGG | - | chr8.2:7931275-7931294 | None:intergenic | 45.0% | |
| GGGGTTTCCCAAGTAAGATT+AGG | + | chr8.2:7930791-7930810 | MS.gene24782:intron | 45.0% | |
| ! | AGTGCTGAAAACCTGCATCA+AGG | + | chr8.2:7931437-7931456 | MS.gene24782:CDS | 45.0% |
| ! | TGAATCAGCCTTTGAGAGTG+TGG | + | chr8.2:7931475-7931494 | MS.gene24782:CDS | 45.0% |
| AGTGGAATGACTTCATGGGC+GGG | - | chr8.2:7930598-7930617 | None:intergenic | 50.0% | |
| CAGTGGAATGACTTCATGGG+CGG | - | chr8.2:7930599-7930618 | None:intergenic | 50.0% | |
| TGAAGGAGGTTCCATACCAG+TGG | - | chr8.2:7930616-7930635 | None:intergenic | 50.0% | |
| CGCCCATGAAGTCATTCCAC+TGG | + | chr8.2:7930597-7930616 | MS.gene24782:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7930479 | 7931613 | 7930479 | ID=MS.gene24782 |
| chr8.2 | mRNA | 7930479 | 7931613 | 7930479 | ID=MS.gene24782.t1;Parent=MS.gene24782 |
| chr8.2 | exon | 7930479 | 7930618 | 7930479 | ID=MS.gene24782.t1.exon1;Parent=MS.gene24782.t1 |
| chr8.2 | CDS | 7930479 | 7930618 | 7930479 | ID=cds.MS.gene24782.t1;Parent=MS.gene24782.t1 |
| chr8.2 | exon | 7931424 | 7931613 | 7931424 | ID=MS.gene24782.t1.exon2;Parent=MS.gene24782.t1 |
| chr8.2 | CDS | 7931424 | 7931613 | 7931424 | ID=cds.MS.gene24782.t1;Parent=MS.gene24782.t1 |
| Gene Sequence |
| Protein sequence |
