Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012190.t1 | XP_003609973.1 | 96.1 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 3.60E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012190.t1 | Q9LUV2 | 71.6 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 5.6e-42 | 171.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012190.t1 | G7JS72 | 96.1 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 2.6e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene012190 | MS.gene90649 | PPI |
| MS.gene43077 | MS.gene012190 | PPI |
| MS.gene35373 | MS.gene012190 | PPI |
| MS.gene073085 | MS.gene012190 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012190.t1 | MTR_4g124920 | 96.117 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 6.88e-69 | 201 |
| MS.gene012190.t1 | MTR_4g124920 | 95.714 | 70 | 3 | 0 | 7 | 76 | 2 | 71 | 3.77e-44 | 139 |
| MS.gene012190.t1 | MTR_2g084985 | 39.216 | 102 | 58 | 2 | 8 | 108 | 7 | 105 | 5.52e-20 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012190.t1 | AT3G17210 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 2.86e-56 | 170 |
| MS.gene012190.t1 | AT3G17210 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 2.86e-56 | 170 |
| MS.gene012190.t1 | AT5G22580 | 39.000 | 100 | 57 | 2 | 4 | 102 | 2 | 98 | 1.44e-22 | 85.1 |
Find 22 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAGCCACATACTCTTTAA+TGG | 0.270013 | 8.1:-7139681 | None:intergenic |
| TACAATCACTTTCTCCAAGT+TGG | 0.390001 | 8.1:-7139738 | None:intergenic |
| AAATTTGTTCCTTTCCAACT+TGG | 0.431812 | 8.1:+7139724 | MS.gene012190:CDS |
| AAAGAAATGGCGGAAGCAAA+AGG | 0.444914 | 8.1:+7138655 | None:intergenic |
| CATAGTGATCGCAAAGTTCA+AGG | 0.471142 | 8.1:+7138690 | MS.gene012190:CDS |
| ATACCAGTGGAATGACTTCA+TGG | 0.474858 | 8.1:-7138782 | None:intergenic |
| GAAGATTGATGAACTCATCA+AGG | 0.501185 | 8.1:+7138729 | MS.gene012190:CDS |
| CATGAGTAAAACCTTGATGC+AGG | 0.509216 | 8.1:-7139628 | None:intergenic |
| TTTGCATACTCAACATGTGC+AGG | 0.522063 | 8.1:-7139704 | None:intergenic |
| CGCCCATGAAGTCATTCCAC+TGG | 0.526302 | 8.1:+7138779 | MS.gene012190:CDS |
| AATGGCTTCCACACTCTCAA+AGG | 0.541657 | 8.1:-7139663 | None:intergenic |
| AATCAAAGATTAACGATGGT+GGG | 0.542487 | 8.1:-7139773 | None:intergenic |
| TGAATCAGCCTTTGAGAGTG+TGG | 0.555458 | 8.1:+7139655 | MS.gene012190:CDS |
| AAATCAAAGATTAACGATGG+TGG | 0.556350 | 8.1:-7139774 | None:intergenic |
| TCACTTTCTCCAAGTTGGAA+AGG | 0.558231 | 8.1:-7139733 | None:intergenic |
| AGTGCTGAAAACCTGCATCA+AGG | 0.585611 | 8.1:+7139617 | MS.gene012190:CDS |
| GGAAGCCATTAAAGAGTATG+TGG | 0.589757 | 8.1:+7139676 | MS.gene012190:CDS |
| AGTGGAATGACTTCATGGGC+GGG | 0.607686 | 8.1:-7138777 | None:intergenic |
| CAGTGGAATGACTTCATGGG+CGG | 0.628089 | 8.1:-7138778 | None:intergenic |
| TACCAGTGGAATGACTTCAT+GGG | 0.633875 | 8.1:-7138781 | None:intergenic |
| AAGATTGATGAACTCATCAA+GGG | 0.636945 | 8.1:+7138730 | MS.gene012190:CDS |
| AATAAATCAAAGATTAACGA+TGG | 0.651109 | 8.1:-7139777 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAATTCATTTAATAATT+GGG | + | chr8.1:7138953-7138972 | MS.gene012190:intron | 10.0% |
| !! | TAGTAAATTCATTTAATAAT+TGG | + | chr8.1:7138952-7138971 | MS.gene012190:intron | 10.0% |
| !! | GTAAATTCATTTAATAATTG+GGG | + | chr8.1:7138954-7138973 | MS.gene012190:intron | 15.0% |
| !! | TAAAACTAATCTATTACATT+AGG | - | chr8.1:7139224-7139243 | None:intergenic | 15.0% |
| !! | TAATAGTACATAATGAATGA+AGG | - | chr8.1:7138815-7138834 | None:intergenic | 20.0% |
| !! | TATCTATTGTAAATATCACT+TGG | - | chr8.1:7139198-7139217 | None:intergenic | 20.0% |
| !!! | AAAATTAAGTCATACAGTTT+TGG | + | chr8.1:7139343-7139362 | MS.gene012190:intron | 20.0% |
| !!! | GTTATCTTTTATTGTGTATA+GGG | + | chr8.1:7139583-7139602 | MS.gene012190:intron | 20.0% |
| !!! | TGTTATCTTTTATTGTGTAT+AGG | + | chr8.1:7139582-7139601 | MS.gene012190:intron | 20.0% |
| !!! | TTATCTTTTATTGTGTATAG+GGG | + | chr8.1:7139584-7139603 | MS.gene012190:intron | 20.0% |
| ! | GTTGTTTACATAAGTGATTA+TGG | + | chr8.1:7139124-7139143 | MS.gene012190:intron | 25.0% |
| !! | TATCTTTTATTGTGTATAGG+GGG | + | chr8.1:7139585-7139604 | MS.gene012190:intron | 25.0% |
| !!! | TATATTGCTAAGCTTTGAAA+TGG | - | chr8.1:7139547-7139566 | None:intergenic | 25.0% |
| AAGATTGATGAACTCATCAA+GGG | + | chr8.1:7138730-7138749 | MS.gene012190:CDS | 30.0% | |
| AGAATCAGTGGAACATATTT+GGG | + | chr8.1:7138996-7139015 | MS.gene012190:intron | 30.0% | |
| CAAATTGATGCTGCAAAAAA+TGG | + | chr8.1:7138903-7138922 | MS.gene012190:intron | 30.0% | |
| TAAACAACAGATGGCAAAAA+AGG | - | chr8.1:7139112-7139131 | None:intergenic | 30.0% | |
| TAGTACATAATGAATGAAGG+AGG | - | chr8.1:7138812-7138831 | None:intergenic | 30.0% | |
| TCACTTATGTAAACAACAGA+TGG | - | chr8.1:7139121-7139140 | None:intergenic | 30.0% | |
| TGATTCTCCTAATCTTACTT+GGG | - | chr8.1:7138983-7139002 | None:intergenic | 30.0% | |
| ! | AAATTTGTTCCTTTCCAACT+TGG | + | chr8.1:7139724-7139743 | MS.gene012190:CDS | 30.0% |
| ! | ACATTAGTCTCAAAAAGACT+AGG | + | chr8.1:7139161-7139180 | MS.gene012190:intron | 30.0% |
| ! | CATTAGTCTCAAAAAGACTA+GGG | + | chr8.1:7139162-7139181 | MS.gene012190:intron | 30.0% |
| AGTAAGATTAGGAGAATCAG+TGG | + | chr8.1:7138984-7139003 | MS.gene012190:intron | 35.0% | |
| ATGAGCCACATACTCTTTAA+TGG | - | chr8.1:7139684-7139703 | None:intergenic | 35.0% | |
| ATGGTAATCATAGCCACTAA+TGG | - | chr8.1:7139528-7139547 | None:intergenic | 35.0% | |
| CAATCTAAACTGACCATTAG+TGG | + | chr8.1:7139512-7139531 | MS.gene012190:intron | 35.0% | |
| CTGATTCTCCTAATCTTACT+TGG | - | chr8.1:7138984-7139003 | None:intergenic | 35.0% | |
| GAAGATTGATGAACTCATCA+AGG | + | chr8.1:7138729-7138748 | MS.gene012190:CDS | 35.0% | |
| GAATCAGTGGAACATATTTG+GGG | + | chr8.1:7138997-7139016 | MS.gene012190:intron | 35.0% | |
| GAGAATCAGTGGAACATATT+TGG | + | chr8.1:7138995-7139014 | MS.gene012190:intron | 35.0% | |
| TACAATCACTTTCTCCAAGT+TGG | - | chr8.1:7139741-7139760 | None:intergenic | 35.0% | |
| TCTCAAAAAGACTAGGGAAA+TGG | + | chr8.1:7139168-7139187 | MS.gene012190:intron | 35.0% | |
| ! | ATATTTGCGCTGATCTAAGT+GGG | - | chr8.1:7138862-7138881 | None:intergenic | 35.0% |
| ! | TTTTATTGTGTATAGGGGGA+CGG | + | chr8.1:7139589-7139608 | MS.gene012190:intron | 35.0% |
| !! | CTTATTTCTATAAGCTCTCC+AGG | - | chr8.1:7139399-7139418 | None:intergenic | 35.0% |
| AAGCGCTTATGAGACTGTTT+GGG | - | chr8.1:7139468-7139487 | None:intergenic | 40.0% | |
| ATACCAGTGGAATGACTTCA+TGG | - | chr8.1:7138785-7138804 | None:intergenic | 40.0% | |
| ATGAAGTCATTCCACTGGTA+TGG | + | chr8.1:7138784-7138803 | MS.gene012190:intron | 40.0% | |
| CATAGTGATCGCAAAGTTCA+AGG | + | chr8.1:7138690-7138709 | MS.gene012190:CDS | 40.0% | |
| CATGAGTAAAACCTTGATGC+AGG | - | chr8.1:7139631-7139650 | None:intergenic | 40.0% | |
| TAAGCGCTTATGAGACTGTT+TGG | - | chr8.1:7139469-7139488 | None:intergenic | 40.0% | |
| TACCAGTGGAATGACTTCAT+GGG | - | chr8.1:7138784-7138803 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGTGC+AGG | - | chr8.1:7139707-7139726 | None:intergenic | 40.0% | |
| ! | GATATTTGCGCTGATCTAAG+TGG | - | chr8.1:7138863-7138882 | None:intergenic | 40.0% |
| ! | GGAAGCCATTAAAGAGTATG+TGG | + | chr8.1:7139676-7139695 | MS.gene012190:CDS | 40.0% |
| ! | TCACTTTCTCCAAGTTGGAA+AGG | - | chr8.1:7139736-7139755 | None:intergenic | 40.0% |
| !!! | GCTGTTTTGATAAGCTATCC+TGG | + | chr8.1:7139378-7139397 | MS.gene012190:intron | 40.0% |
| AATGGCTTCCACACTCTCAA+AGG | - | chr8.1:7139666-7139685 | None:intergenic | 45.0% | |
| AGACTGTTTGGGAGAGCTTA+TGG | - | chr8.1:7139457-7139476 | None:intergenic | 45.0% | |
| GGGGTTTCCCAAGTAAGATT+AGG | + | chr8.1:7138973-7138992 | MS.gene012190:intron | 45.0% | |
| ! | AGTGCTGAAAACCTGCATCA+AGG | + | chr8.1:7139617-7139636 | MS.gene012190:CDS | 45.0% |
| ! | TGAATCAGCCTTTGAGAGTG+TGG | + | chr8.1:7139655-7139674 | MS.gene012190:CDS | 45.0% |
| AGTGGAATGACTTCATGGGC+GGG | - | chr8.1:7138780-7138799 | None:intergenic | 50.0% | |
| CAGTGGAATGACTTCATGGG+CGG | - | chr8.1:7138781-7138800 | None:intergenic | 50.0% | |
| TGAAGGAGGTTCCATACCAG+TGG | - | chr8.1:7138798-7138817 | None:intergenic | 50.0% | |
| CGCCCATGAAGTCATTCCAC+TGG | + | chr8.1:7138779-7138798 | MS.gene012190:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 7138661 | 7139793 | 7138661 | ID=MS.gene012190 |
| chr8.1 | mRNA | 7138661 | 7139793 | 7138661 | ID=MS.gene012190.t1;Parent=MS.gene012190 |
| chr8.1 | exon | 7138661 | 7138800 | 7138661 | ID=MS.gene012190.t1.exon1;Parent=MS.gene012190.t1 |
| chr8.1 | CDS | 7138661 | 7138800 | 7138661 | ID=cds.MS.gene012190.t1;Parent=MS.gene012190.t1 |
| chr8.1 | exon | 7139604 | 7139793 | 7139604 | ID=MS.gene012190.t1.exon2;Parent=MS.gene012190.t1 |
| chr8.1 | CDS | 7139604 | 7139793 | 7139604 | ID=cds.MS.gene012190.t1;Parent=MS.gene012190.t1 |
| Gene Sequence |
| Protein sequence |
