Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25266.t1 | XP_003609973.1 | 96.1 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 3.60E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25266.t1 | Q9LUV2 | 71.6 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 5.6e-42 | 171.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25266.t1 | G7JS72 | 96.1 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 2.6e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene35373 | MS.gene25266 | PPI |
| MS.gene25266 | MS.gene90649 | PPI |
| MS.gene25266 | MS.gene073085 | PPI |
| MS.gene25266 | MS.gene43077 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25266.t1 | MTR_4g124920 | 96.117 | 103 | 4 | 0 | 7 | 109 | 2 | 104 | 6.88e-69 | 201 |
| MS.gene25266.t1 | MTR_4g124920 | 95.714 | 70 | 3 | 0 | 7 | 76 | 2 | 71 | 3.77e-44 | 139 |
| MS.gene25266.t1 | MTR_2g084985 | 39.216 | 102 | 58 | 2 | 8 | 108 | 7 | 105 | 5.52e-20 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25266.t1 | AT3G17210 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 2.86e-56 | 170 |
| MS.gene25266.t1 | AT3G17210 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 2.86e-56 | 170 |
| MS.gene25266.t1 | AT5G22580 | 39.000 | 100 | 57 | 2 | 4 | 102 | 2 | 98 | 1.44e-22 | 85.1 |
Find 22 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAGCCACATACTCTTTAA+TGG | 0.270013 | 8.2:-7901059 | None:intergenic |
| TACAATCACTTTCTCCAAGT+TGG | 0.390001 | 8.2:-7901116 | None:intergenic |
| AAATTTGTTCCTTTCCAACT+TGG | 0.431812 | 8.2:+7901102 | MS.gene25266:CDS |
| AAAGAAATGGCGGAAGCAAA+AGG | 0.444914 | 8.2:+7900031 | None:intergenic |
| CATAGTGATCGCAAAGTTCA+AGG | 0.471142 | 8.2:+7900066 | MS.gene25266:CDS |
| ATACCAGTGGAATGACTTCA+TGG | 0.474858 | 8.2:-7900158 | None:intergenic |
| GAAGATTGATGAACTCATCA+AGG | 0.501185 | 8.2:+7900105 | MS.gene25266:CDS |
| CATGAGTAAAACCTTGATGC+AGG | 0.509216 | 8.2:-7901006 | None:intergenic |
| TTTGCATACTCAACATGTGC+AGG | 0.522063 | 8.2:-7901082 | None:intergenic |
| CGCCCATGAAGTCATTCCAC+TGG | 0.526302 | 8.2:+7900155 | MS.gene25266:CDS |
| AATGGCTTCCACACTCTCAA+AGG | 0.541657 | 8.2:-7901041 | None:intergenic |
| AATCAAAGATTAACGATGGT+GGG | 0.542487 | 8.2:-7901151 | None:intergenic |
| TGAATCAGCCTTTGAGAGTG+TGG | 0.555458 | 8.2:+7901033 | MS.gene25266:CDS |
| AAATCAAAGATTAACGATGG+TGG | 0.556350 | 8.2:-7901152 | None:intergenic |
| TCACTTTCTCCAAGTTGGAA+AGG | 0.558231 | 8.2:-7901111 | None:intergenic |
| AGTGCTGAAAACCTGCATCA+AGG | 0.585611 | 8.2:+7900995 | MS.gene25266:CDS |
| GGAAGCCATTAAAGAGTATG+TGG | 0.589757 | 8.2:+7901054 | MS.gene25266:CDS |
| AGTGGAATGACTTCATGGGC+GGG | 0.607686 | 8.2:-7900153 | None:intergenic |
| CAGTGGAATGACTTCATGGG+CGG | 0.628089 | 8.2:-7900154 | None:intergenic |
| TACCAGTGGAATGACTTCAT+GGG | 0.633875 | 8.2:-7900157 | None:intergenic |
| AAGATTGATGAACTCATCAA+GGG | 0.636945 | 8.2:+7900106 | MS.gene25266:CDS |
| AATAAATCAAAGATTAACGA+TGG | 0.651109 | 8.2:-7901155 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAATTCATTTAATAATT+GGG | + | chr8.2:7900329-7900348 | MS.gene25266:intron | 10.0% |
| !! | TAGTAAATTCATTTAATAAT+TGG | + | chr8.2:7900328-7900347 | MS.gene25266:intron | 10.0% |
| !! | GTAAATTCATTTAATAATTG+GGG | + | chr8.2:7900330-7900349 | MS.gene25266:intron | 15.0% |
| !! | TAAAACTAATCTATTACATT+AGG | - | chr8.2:7900600-7900619 | None:intergenic | 15.0% |
| !! | TAATAGTACATAATGAATGA+AGG | - | chr8.2:7900191-7900210 | None:intergenic | 20.0% |
| !! | TATCTATTGTAAATATCACT+TGG | - | chr8.2:7900574-7900593 | None:intergenic | 20.0% |
| !!! | AAAATTAAGTCATACAGTTT+TGG | + | chr8.2:7900719-7900738 | MS.gene25266:intron | 20.0% |
| !!! | GTTATCTTTTATTGTGTATA+GGG | + | chr8.2:7900961-7900980 | MS.gene25266:intron | 20.0% |
| !!! | TGTTATCTTTTATTGTGTAT+AGG | + | chr8.2:7900960-7900979 | MS.gene25266:intron | 20.0% |
| !!! | TTATCTTTTATTGTGTATAG+GGG | + | chr8.2:7900962-7900981 | MS.gene25266:intron | 20.0% |
| ! | GTTGTTTACATAAGTGATTA+TGG | + | chr8.2:7900500-7900519 | MS.gene25266:intron | 25.0% |
| !! | TATCTTTTATTGTGTATAGG+GGG | + | chr8.2:7900963-7900982 | MS.gene25266:intron | 25.0% |
| AAGATTGATGAACTCATCAA+GGG | + | chr8.2:7900106-7900125 | MS.gene25266:CDS | 30.0% | |
| AGAATCAGTGGAACATATTT+GGG | + | chr8.2:7900372-7900391 | MS.gene25266:intron | 30.0% | |
| CAAATTGATGCTGCAAAAAA+TGG | + | chr8.2:7900279-7900298 | MS.gene25266:intron | 30.0% | |
| TAAACAACAGATGGCAAAAA+AGG | - | chr8.2:7900488-7900507 | None:intergenic | 30.0% | |
| TAGTACATAATGAATGAAGG+AGG | - | chr8.2:7900188-7900207 | None:intergenic | 30.0% | |
| TCACTTATGTAAACAACAGA+TGG | - | chr8.2:7900497-7900516 | None:intergenic | 30.0% | |
| TGATTCTCCTAATCTTACTT+GGG | - | chr8.2:7900359-7900378 | None:intergenic | 30.0% | |
| ! | AAATTTGTTCCTTTCCAACT+TGG | + | chr8.2:7901102-7901121 | MS.gene25266:CDS | 30.0% |
| ! | ACATTAGTCTCAAAAAGACT+AGG | + | chr8.2:7900537-7900556 | MS.gene25266:intron | 30.0% |
| ! | CATTAGTCTCAAAAAGACTA+GGG | + | chr8.2:7900538-7900557 | MS.gene25266:intron | 30.0% |
| AGTAAGATTAGGAGAATCAG+TGG | + | chr8.2:7900360-7900379 | MS.gene25266:intron | 35.0% | |
| ATGAGCCACATACTCTTTAA+TGG | - | chr8.2:7901062-7901081 | None:intergenic | 35.0% | |
| CAATCTAAACTGACCATTAG+TGG | + | chr8.2:7900888-7900907 | MS.gene25266:intron | 35.0% | |
| CTGATTCTCCTAATCTTACT+TGG | - | chr8.2:7900360-7900379 | None:intergenic | 35.0% | |
| GAAGATTGATGAACTCATCA+AGG | + | chr8.2:7900105-7900124 | MS.gene25266:CDS | 35.0% | |
| GAATCAGTGGAACATATTTG+GGG | + | chr8.2:7900373-7900392 | MS.gene25266:intron | 35.0% | |
| GAGAATCAGTGGAACATATT+TGG | + | chr8.2:7900371-7900390 | MS.gene25266:intron | 35.0% | |
| GTTGTAATCATAGCCACTAA+TGG | - | chr8.2:7900904-7900923 | None:intergenic | 35.0% | |
| TACAATCACTTTCTCCAAGT+TGG | - | chr8.2:7901119-7901138 | None:intergenic | 35.0% | |
| TCTCAAAAAGACTAGGGAAA+TGG | + | chr8.2:7900544-7900563 | MS.gene25266:intron | 35.0% | |
| ! | ATATTTGCGCTGATCTAAGT+GGG | - | chr8.2:7900238-7900257 | None:intergenic | 35.0% |
| ! | TTTTATTGTGTATAGGGGGA+CGG | + | chr8.2:7900967-7900986 | MS.gene25266:intron | 35.0% |
| !! | CTTATTTCTATAAGCTCTCC+AGG | - | chr8.2:7900775-7900794 | None:intergenic | 35.0% |
| AAGCGCTTATGAGACTGTTT+GGG | - | chr8.2:7900844-7900863 | None:intergenic | 40.0% | |
| ATACCAGTGGAATGACTTCA+TGG | - | chr8.2:7900161-7900180 | None:intergenic | 40.0% | |
| ATGAAGTCATTCCACTGGTA+TGG | + | chr8.2:7900160-7900179 | MS.gene25266:intron | 40.0% | |
| CATAGTGATCGCAAAGTTCA+AGG | + | chr8.2:7900066-7900085 | MS.gene25266:CDS | 40.0% | |
| CATGAGTAAAACCTTGATGC+AGG | - | chr8.2:7901009-7901028 | None:intergenic | 40.0% | |
| TAAGCGCTTATGAGACTGTT+TGG | - | chr8.2:7900845-7900864 | None:intergenic | 40.0% | |
| TACCAGTGGAATGACTTCAT+GGG | - | chr8.2:7900160-7900179 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGTGC+AGG | - | chr8.2:7901085-7901104 | None:intergenic | 40.0% | |
| ! | GATATTTGCGCTGATCTAAG+TGG | - | chr8.2:7900239-7900258 | None:intergenic | 40.0% |
| ! | GGAAGCCATTAAAGAGTATG+TGG | + | chr8.2:7901054-7901073 | MS.gene25266:CDS | 40.0% |
| ! | TCACTTTCTCCAAGTTGGAA+AGG | - | chr8.2:7901114-7901133 | None:intergenic | 40.0% |
| !!! | GCTGTTTTGATAAGCTATCC+TGG | + | chr8.2:7900754-7900773 | MS.gene25266:intron | 40.0% |
| AATGGCTTCCACACTCTCAA+AGG | - | chr8.2:7901044-7901063 | None:intergenic | 45.0% | |
| AGACTGTTTGGGAGAGCTTA+TGG | - | chr8.2:7900833-7900852 | None:intergenic | 45.0% | |
| GGGGTTTCCCAAGTAAGATT+AGG | + | chr8.2:7900349-7900368 | MS.gene25266:intron | 45.0% | |
| ! | AGTGCTGAAAACCTGCATCA+AGG | + | chr8.2:7900995-7901014 | MS.gene25266:CDS | 45.0% |
| ! | TGAATCAGCCTTTGAGAGTG+TGG | + | chr8.2:7901033-7901052 | MS.gene25266:CDS | 45.0% |
| AGTGGAATGACTTCATGGGC+GGG | - | chr8.2:7900156-7900175 | None:intergenic | 50.0% | |
| CAGTGGAATGACTTCATGGG+CGG | - | chr8.2:7900157-7900176 | None:intergenic | 50.0% | |
| TGAAGGAGGTTCCATACCAG+TGG | - | chr8.2:7900174-7900193 | None:intergenic | 50.0% | |
| CGCCCATGAAGTCATTCCAC+TGG | + | chr8.2:7900155-7900174 | MS.gene25266:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7900037 | 7901171 | 7900037 | ID=MS.gene25266 |
| chr8.2 | mRNA | 7900037 | 7901171 | 7900037 | ID=MS.gene25266.t1;Parent=MS.gene25266 |
| chr8.2 | exon | 7900037 | 7900176 | 7900037 | ID=MS.gene25266.t1.exon1;Parent=MS.gene25266.t1 |
| chr8.2 | CDS | 7900037 | 7900176 | 7900037 | ID=cds.MS.gene25266.t1;Parent=MS.gene25266.t1 |
| chr8.2 | exon | 7900982 | 7901171 | 7900982 | ID=MS.gene25266.t1.exon2;Parent=MS.gene25266.t1 |
| chr8.2 | CDS | 7900982 | 7901171 | 7900982 | ID=cds.MS.gene25266.t1;Parent=MS.gene25266.t1 |
| Gene Sequence |
| Protein sequence |
