AlfalfaGEDB | MS.gene43585

M. sativa cultivar XinJiangDaYe / MS.gene43585

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43585.t1	XP_003593866.1	97.6	246	6	0	1	246	1	246	1.80E-132	481.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43585.t1	G7IP32	97.6	246	6	0	1	246	1	246	1.3e-132	481.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055147	MS.gene43585	0.800154	1.65E-48	-1.69E-46
MS.gene055148	MS.gene43585	0.817149	3.83E-52	-1.69E-46
MS.gene05808	MS.gene43585	0.992616	2.25E-194	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43585.t1	MTR_2g018670	97.561	246	6	0	1	246	1	246	0.0	501
MS.gene43585.t1	MTR_2g018670	96.296	189	7	0	58	246	3	191	6.94e-137	382
MS.gene43585.t1	MTR_2g018670	97.561	164	4	0	83	246	1	164	1.47e-119	338
MS.gene43585.t1	MTR_8g079475	65.726	248	82	3	1	246	2	248	3.22e-118	338

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 38 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCATCAGATGTTAGAAAA+TGG	0.212688	2.2:+66030629	MS.gene43585:CDS
TAGAAGCATCCATGGAAATA+TGG	0.347628	2.2:+66029359	MS.gene43585:CDS
ATGGCAGAAGCAATCTATTA+AGG	0.348198	2.2:+66029990	MS.gene43585:CDS
AAAATAGAAGGCATGACTTC+AGG	0.371700	2.2:+66030398	MS.gene43585:CDS
ACATGAATGCACACAACTAT+TGG	0.377348	2.2:-66030446	None:intergenic
ATTATAGATGATCTTGTTCA+TGG	0.379410	2.2:+66029279	MS.gene43585:CDS
TTTCATGTAATCAGATCATA+TGG	0.381243	2.2:+66029959	MS.gene43585:intron
TTCAAAGTAGTGTATGTTAG+TGG	0.388260	2.2:+66030575	MS.gene43585:CDS
ACCAAGGGTTTCAGTAGTTA+AGG	0.397101	2.2:-66029316	None:intergenic
ATTCTCCAGCATATGCTTCA+AGG	0.397395	2.2:-66030241	None:intergenic
TCATCTATAATCACAATTGT+TGG	0.398129	2.2:-66029267	None:intergenic
ATCATCAGATGTTAGAAAAT+GGG	0.425313	2.2:+66030630	MS.gene43585:CDS
TTAATTCTGACCATAGGAAA+TGG	0.426007	2.2:+66030504	MS.gene43585:CDS
TCACGCTTTGGGAAAATAGA+AGG	0.429485	2.2:+66030386	MS.gene43585:CDS
CGGCGTAAGAAAGGTCATTA+AGG	0.433721	2.2:-66030346	None:intergenic
TCAGCTGCAAAATTCTCAAC+TGG	0.441332	2.2:+66030278	MS.gene43585:CDS
ACTTCAGGTCTTTGCTTGAA+AGG	0.470876	2.2:+66030413	MS.gene43585:CDS
GAGCTAACACGGCGTAAGAA+AGG	0.484665	2.2:-66030355	None:intergenic
AGTTTCCTTGAAGCATATGC+TGG	0.496553	2.2:+66030236	MS.gene43585:CDS
TCCTTAACTACTGAAACCCT+TGG	0.503695	2.2:+66029315	MS.gene43585:CDS
GTCATCTCTACTATTCGTGC+CGG	0.513748	2.2:-66030324	None:intergenic
TGTAATCAGATCATATGGTA+TGG	0.530296	2.2:+66029964	MS.gene43585:intron
AGGAATTTACCATATTTCCA+TGG	0.534844	2.2:-66029368	None:intergenic
AGATGTTAGAAAATGGGAAA+GGG	0.535212	2.2:+66030636	MS.gene43585:CDS
AAGGCATCATCCATTTCCTA+TGG	0.542944	2.2:-66030514	None:intergenic
TCTATATTTAGAAGCATCCA+TGG	0.553025	2.2:+66029351	MS.gene43585:CDS
CAGATGTTAGAAAATGGGAA+AGG	0.557087	2.2:+66030635	MS.gene43585:CDS
ATATTATCTATCAACTCAGC+CGG	0.573023	2.2:+66030305	MS.gene43585:CDS
GGATACTTAATTCTGACCAT+AGG	0.582839	2.2:+66030498	MS.gene43585:CDS
AGAGCTTTGAACACAACCAA+GGG	0.600437	2.2:-66029331	None:intergenic
AGATCATATGGTATGGAGCA+TGG	0.601542	2.2:+66029971	MS.gene43585:intron
AAAGGATATGGCATGTTCTG+TGG	0.607383	2.2:+66029200	None:intergenic
ACAGAAATCAATGTTAACCA+AGG	0.624182	2.2:+66030184	MS.gene43585:intron
TAGAGCTTTGAACACAACCA+AGG	0.633504	2.2:-66029332	None:intergenic
GTTAACCAAGGTATCAACCA+TGG	0.651380	2.2:+66030196	MS.gene43585:CDS
TATGCTGGAGAATCAAGAGA+TGG	0.654209	2.2:+66030251	MS.gene43585:CDS
TGACCTGAAAAGAGCTAACA+CGG	0.676515	2.2:-66030366	None:intergenic
TAGTTGTGTGCATTCATGTG+TGG	0.691763	2.2:+66030450	MS.gene43585:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTATTAGAATAAATTAAAA+TGG	-	chr2.2:66030145-66030164	None:intergenic	10.0%
!!	AGTAAAATTGAATTGAAAAT+TGG	-	chr2.2:66029412-66029431	None:intergenic	15.0%
!!	ATTAGAATAAATTAAAATGG+AGG	-	chr2.2:66030142-66030161	None:intergenic	15.0%
!!	CATATAATATCATCTTTATT+TGG	+	chr2.2:66029596-66029615	MS.gene43585:intron	15.0%
!!	TTAGAATAAATTAAAATGGA+GGG	-	chr2.2:66030141-66030160	None:intergenic	15.0%
!!!	ACAATTTTTAAATTCAAGTT+AGG	-	chr2.2:66029808-66029827	None:intergenic	15.0%
!!!	AAAAATGATTTTGGTATGTT+TGG	+	chr2.2:66029761-66029780	MS.gene43585:intron	20.0%
!!!	TGAAGACTAAAAAATGATTT+TGG	+	chr2.2:66029752-66029771	MS.gene43585:intron	20.0%
!	ATCATCAGATGTTAGAAAAT+GGG	+	chr2.2:66030630-66030649	MS.gene43585:CDS	25.0%
!	ATTATAGATGATCTTGTTCA+TGG	+	chr2.2:66029279-66029298	MS.gene43585:CDS	25.0%
!	TAAATTCACAATGGAAGAAA+TGG	-	chr2.2:66029528-66029547	None:intergenic	25.0%
!	TCATCTATAATCACAATTGT+TGG	-	chr2.2:66029270-66029289	None:intergenic	25.0%
!	TTTCATGTAATCAGATCATA+TGG	+	chr2.2:66029959-66029978	MS.gene43585:intron	25.0%
!!	ATGTTCAATTAATTTTGCCA+TGG	-	chr2.2:66030216-66030235	None:intergenic	25.0%
!!	TCATGTTTGGATTCAATTTT+GGG	+	chr2.2:66029712-66029731	MS.gene43585:intron	25.0%
!!	TTCATGTTTGGATTCAATTT+TGG	+	chr2.2:66029711-66029730	MS.gene43585:intron	25.0%
	ACAGAAATCAATGTTAACCA+AGG	+	chr2.2:66030184-66030203	MS.gene43585:intron	30.0%
	AGATGTTAGAAAATGGGAAA+GGG	+	chr2.2:66030636-66030655	MS.gene43585:CDS	30.0%
	AGGAATTTACCATATTTCCA+TGG	-	chr2.2:66029371-66029390	None:intergenic	30.0%
	ATATTATCTATCAACTCAGC+CGG	+	chr2.2:66030305-66030324	MS.gene43585:CDS	30.0%
	ATGCATACAAAACATCTGAA+AGG	-	chr2.2:66030060-66030079	None:intergenic	30.0%
	ATGGAGGGAAATAATTGATT+TGG	-	chr2.2:66030126-66030145	None:intergenic	30.0%
	CAGCAAAGTTTCAAACAAAA+GGG	-	chr2.2:66029488-66029507	None:intergenic	30.0%
	CATCATCAGATGTTAGAAAA+TGG	+	chr2.2:66030629-66030648	MS.gene43585:CDS	30.0%
	TCAGCAAAGTTTCAAACAAA+AGG	-	chr2.2:66029489-66029508	None:intergenic	30.0%
	TGTAATCAGATCATATGGTA+TGG	+	chr2.2:66029964-66029983	MS.gene43585:intron	30.0%
	TTAATTCTGACCATAGGAAA+TGG	+	chr2.2:66030504-66030523	MS.gene43585:CDS	30.0%
	TTCAAAGTAGTGTATGTTAG+TGG	+	chr2.2:66030575-66030594	MS.gene43585:CDS	30.0%
	TTTGCAGCATAAATTCACAA+TGG	-	chr2.2:66029537-66029556	None:intergenic	30.0%
!	ATTTATGCTGCAAATTTGCT+TGG	+	chr2.2:66029543-66029562	MS.gene43585:intron	30.0%
!	GGCTTTTAACAACAAGAATT+TGG	+	chr2.2:66029221-66029240	MS.gene43585:CDS	30.0%
!!	CTCTTCATTTTTGTTACTCA+TGG	+	chr2.2:66030477-66030496	MS.gene43585:CDS	30.0%
!!	TCATCAAAAGTTTAGTTTGC+TGG	+	chr2.2:66029635-66029654	MS.gene43585:intron	30.0%
!!	TCTATATTTAGAAGCATCCA+TGG	+	chr2.2:66029351-66029370	MS.gene43585:CDS	30.0%
!!!	ATCGCGAATTTTTCAAGATA+AGG	-	chr2.2:66030536-66030555	None:intergenic	30.0%
	AATCCAAACATGAACTACCA+TGG	-	chr2.2:66029705-66029724	None:intergenic	35.0%
	ACATGAATGCACACAACTAT+TGG	-	chr2.2:66030449-66030468	None:intergenic	35.0%
	ATGGCAGAAGCAATCTATTA+AGG	+	chr2.2:66029990-66030009	MS.gene43585:CDS	35.0%
	CAGATGTTAGAAAATGGGAA+AGG	+	chr2.2:66030635-66030654	MS.gene43585:CDS	35.0%
	GGAAATCAGAGAAAGAAAAC+AGG	-	chr2.2:66029391-66029410	None:intergenic	35.0%
	GGATACTTAATTCTGACCAT+AGG	+	chr2.2:66030498-66030517	MS.gene43585:CDS	35.0%
!	AAAATAGAAGGCATGACTTC+AGG	+	chr2.2:66030398-66030417	MS.gene43585:CDS	35.0%
!!	ACAATGCGTGTTTGTTTCTA+CGG	-	chr2.2:66029912-66029931	None:intergenic	35.0%
!!	TAGAAGCATCCATGGAAATA+TGG	+	chr2.2:66029359-66029378	MS.gene43585:CDS	35.0%
	ACCAAGGGTTTCAGTAGTTA+AGG	-	chr2.2:66029319-66029338	None:intergenic	40.0%
	ACTTCAGGTCTTTGCTTGAA+AGG	+	chr2.2:66030413-66030432	MS.gene43585:CDS	40.0%
	AGATCATATGGTATGGAGCA+TGG	+	chr2.2:66029971-66029990	MS.gene43585:intron	40.0%
	AGTTTCCTTGAAGCATATGC+TGG	+	chr2.2:66030236-66030255	MS.gene43585:CDS	40.0%
	ATTCTCCAGCATATGCTTCA+AGG	-	chr2.2:66030244-66030263	None:intergenic	40.0%
	TAGTTGTGTGCATTCATGTG+TGG	+	chr2.2:66030450-66030469	MS.gene43585:CDS	40.0%
	TATGCTGGAGAATCAAGAGA+TGG	+	chr2.2:66030251-66030270	MS.gene43585:CDS	40.0%
	TCAGCTGCAAAATTCTCAAC+TGG	+	chr2.2:66030278-66030297	MS.gene43585:CDS	40.0%
	TCCTTAACTACTGAAACCCT+TGG	+	chr2.2:66029315-66029334	MS.gene43585:CDS	40.0%
	TGACCTGAAAAGAGCTAACA+CGG	-	chr2.2:66030369-66030388	None:intergenic	40.0%
!	AAGGCATCATCCATTTCCTA+TGG	-	chr2.2:66030517-66030536	None:intergenic	40.0%
!	CTGAAGTTGTCATTTGCTGA+AGG	-	chr2.2:66029671-66029690	None:intergenic	40.0%
!	CTGCAAATTTGCTTGGAAGT+TGG	+	chr2.2:66029550-66029569	MS.gene43585:intron	40.0%
!	GTTAACCAAGGTATCAACCA+TGG	+	chr2.2:66030196-66030215	MS.gene43585:CDS	40.0%
!	TGTGTAAAGCTCACGTTTTG+AGG	-	chr2.2:66029839-66029858	None:intergenic	40.0%
!!	AGAGCTTTGAACACAACCAA+GGG	-	chr2.2:66029334-66029353	None:intergenic	40.0%
!!	TAGAGCTTTGAACACAACCA+AGG	-	chr2.2:66029335-66029354	None:intergenic	40.0%
!!	TCACGCTTTGGGAAAATAGA+AGG	+	chr2.2:66030386-66030405	MS.gene43585:CDS	40.0%
!!	TTTTGCCATGGTTGATACCT+TGG	-	chr2.2:66030204-66030223	None:intergenic	40.0%
	CATGAACTACCATGGAGCAA+TGG	-	chr2.2:66029697-66029716	None:intergenic	45.0%
	CGGCGTAAGAAAGGTCATTA+AGG	-	chr2.2:66030349-66030368	None:intergenic	45.0%
	GCTCCATGGTAGTTCATGTT+TGG	+	chr2.2:66029699-66029718	MS.gene43585:intron	45.0%
	GTCATCTCTACTATTCGTGC+CGG	-	chr2.2:66030327-66030346	None:intergenic	45.0%
!!!	CTCTTTTCAGGTCACGCTTT+GGG	+	chr2.2:66030375-66030394	MS.gene43585:CDS	45.0%
	CTTCAGATGCCATTGCTCCA+TGG	+	chr2.2:66029685-66029704	MS.gene43585:intron	50.0%
	GAGCTAACACGGCGTAAGAA+AGG	-	chr2.2:66030358-66030377	None:intergenic	50.0%
!!	ACGCCGTGTTAGCTCTTTTC+AGG	+	chr2.2:66030363-66030382	MS.gene43585:CDS	50.0%
!!!	GCTCTTTTCAGGTCACGCTT+TGG	+	chr2.2:66030374-66030393	MS.gene43585:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	66029207	66030688	66029207	ID=MS.gene43585
chr2.2	mRNA	66029207	66030688	66029207	ID=MS.gene43585.t1;Parent=MS.gene43585
chr2.2	exon	66029207	66029380	66029207	ID=MS.gene43585.t1.exon1;Parent=MS.gene43585.t1
chr2.2	CDS	66029207	66029380	66029207	ID=cds.MS.gene43585.t1;Parent=MS.gene43585.t1
chr2.2	exon	66029973	66030038	66029973	ID=MS.gene43585.t1.exon2;Parent=MS.gene43585.t1
chr2.2	CDS	66029973	66030038	66029973	ID=cds.MS.gene43585.t1;Parent=MS.gene43585.t1
chr2.2	exon	66030188	66030688	66030188	ID=MS.gene43585.t1.exon3;Parent=MS.gene43585.t1
chr2.2	CDS	66030188	66030688	66030188	ID=cds.MS.gene43585.t1;Parent=MS.gene43585.t1

Gene Sequence

>MS.gene43585

ATGGCATGTTCTGTGGCTTTTAACAACAAGAATTTGGAAATAAGTTTCTTTGTTTTCAAGCCAACAATTGTGATTATAGATGATCTTGTTCATGGTCTCAAACAATTCTCCTTAACTACTGAAACCCTTGGTTGTGTTCAAAGCTCTATATTTAGAAGCATCCATGGAAATATGGTAAATTCCTGTTTTCTTTCTCTGATTTCCAATTTTCAATTCAATTTTACTAGTTTATTTATTCTAAACTAATTGTCTCAATTCTAAAGTGTGTATATATATGTCCCTTTTGTTTGAAACTTTGCTGAATATAGTTTGTGAAATCCATTTCTTCCATTGTGAATTTATGCTGCAAATTTGCTTGGAAGTTGGAAAACATTAACTGAAAGATTGATCATATAATATCATCTTTATTTGGACTCACAAACTCATGTTCATCAAAAGTTTAGTTTGCTGGCAAGTTTTATCCTTCAGCAAATGACAACTTCAGATGCCATTGCTCCATGGTAGTTCATGTTTGGATTCAATTTTGGGAGAGTCAAAAGTGATTCTGAAGACTAAAAAATGATTTTGGTATGTTTGGAGATTCGTGATTAGAAATTATCCTAACTTGAATTTAAAAATTGTAGCTTTTGCCTCAAAACGTGAGCTTTACACACAAACTTATTTATTGTTCAACTCACTTTCAACTAAAATCATTTTTCATCGCCGTAGAAACAAACACGCATTGTTATCGTTGCTCACTTTCATTTCATTTGTTTCATGTAATCAGATCATATGGTATGGAGCATGGCAGAAGCAATCTATTAAGGAAAAAGAGCTTCTAACTTCAACTCTTGTAAGATAAATTTATTTTCCTTTCAGATGTTTTGTATGCATCTTTTCTACTGATTTGTGTTATCTTACAGATTGATTAAGCATACCAAATCAATTATTTCCCTCCATTTTAATTTATTCTAATACAACAACTTTTTGTGTAATTTACAGAAATCAATGTTAACCAAGGTATCAACCATGGCAAAATTAATTGAACATAGTTTCCTTGAAGCATATGCTGGAGAATCAAGAGATGGCTCATCAGCTGCAAAATTCTCAACTGGAGACATATTATCTATCAACTCAGCCGGCACGAATAGTAGAGATGACCTTAATGACCTTTCTTACGCCGTGTTAGCTCTTTTCAGGTCACGCTTTGGGAAAATAGAAGGCATGACTTCAGGTCTTTGCTTGAAAGGACAAAGCAGACCAATAGTTGTGTGCATTCATGTGTGGAAATCTCTTCATTTTTGTTACTCATGGATACTTAATTCTGACCATAGGAAATGGATGATGCCTTATCTTGAAAAATTCGCGATCGATATGAAGTATGATATTTTCAAAGTAGTGTATGTTAGTGGTGACAATGTTGTTGATGTTAACTATATTTCTCATCATCAGATGTTAGAAAATGGGAAAGGGAGCATAGAAAGACAGATAATGCAGAACTGA

Protein sequence

>MS.gene43585.t1

MACSVAFNNKNLEISFFVFKPTIVIIDDLVHGLKQFSLTTETLGCVQSSIFRSIHGNMIIWYGAWQKQSIKEKELLTSTLKSMLTKVSTMAKLIEHSFLEAYAGESRDGSSAAKFSTGDILSINSAGTNSRDDLNDLSYAVLALFRSRFGKIEGMTSGLCLKGQSRPIVVCIHVWKSLHFCYSWILNSDHRKWMMPYLEKFAIDMKYDIFKVVYVSGDNVVDVNYISHHQMLENGKGSIERQIMQN