Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05808.t1 | XP_003593866.1 | 97.6 | 246 | 6 | 0 | 1 | 246 | 1 | 246 | 1.80E-132 | 481.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05808.t1 | G7IP32 | 97.6 | 246 | 6 | 0 | 1 | 246 | 1 | 246 | 1.3e-132 | 481.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055148 | MS.gene05808 | 0.814969 | 1.18E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene05808 | 0.801469 | 8.88E-49 | -1.69E-46 |
| MS.gene05808 | MS.gene27204 | 0.82255 | 2.23E-53 | -1.69E-46 |
| MS.gene05808 | MS.gene34516 | 0.807763 | 4.31E-50 | -1.69E-46 |
| MS.gene05808 | MS.gene43585 | 0.992616 | 2.25E-194 | -1.69E-46 |
| MS.gene05808 | MS.gene46054 | 0.807186 | 5.72E-50 | -1.69E-46 |
| MS.gene05808 | MS.gene66422 | 0.81939 | 1.19E-52 | -1.69E-46 |
| MS.gene05808 | MS.gene89256 | 0.81082 | 9.53E-51 | -1.69E-46 |
| MS.gene05808 | MS.gene94266 | 0.805057 | 1.60E-49 | -1.69E-46 |
| MS.gene05808 | MS.gene94268 | 0.813902 | 2.02E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05808.t1 | MTR_2g018670 | 97.561 | 246 | 6 | 0 | 1 | 246 | 1 | 246 | 0.0 | 501 |
| MS.gene05808.t1 | MTR_2g018670 | 96.296 | 189 | 7 | 0 | 58 | 246 | 3 | 191 | 6.94e-137 | 382 |
| MS.gene05808.t1 | MTR_2g018670 | 97.561 | 164 | 4 | 0 | 83 | 246 | 1 | 164 | 1.47e-119 | 338 |
| MS.gene05808.t1 | MTR_8g079475 | 65.726 | 248 | 82 | 3 | 1 | 246 | 2 | 248 | 3.22e-118 | 338 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCATCAGATGTTAGAAAA+TGG | 0.212688 | 2.3:-67913212 | MS.gene05808:CDS |
| TAGAAGCATCCATGGAAATA+TGG | 0.347628 | 2.3:-67914484 | MS.gene05808:intron |
| ATGGCAGAAGCAATCTATTA+AGG | 0.348198 | 2.3:-67913851 | MS.gene05808:CDS |
| AAAATAGAAGGCATGACTTC+AGG | 0.371700 | 2.3:-67913443 | MS.gene05808:CDS |
| ACATGAATGCACACAACTAT+TGG | 0.377348 | 2.3:+67913395 | None:intergenic |
| ATTATAGATGATCTTGTTCA+TGG | 0.379410 | 2.3:-67914564 | MS.gene05808:CDS |
| TTTCATGTAATCAGATCATA+TGG | 0.381243 | 2.3:-67913882 | MS.gene05808:intron |
| TTCAAAGTAGTGTATGTTAG+TGG | 0.388260 | 2.3:-67913266 | MS.gene05808:CDS |
| ACCAAGGGTTTCAGTAGTTA+AGG | 0.397101 | 2.3:+67914527 | None:intergenic |
| ATTCTCCAGCATATGCTTCA+AGG | 0.397395 | 2.3:+67913600 | None:intergenic |
| TCATCTATAATCACAATTGT+TGG | 0.398129 | 2.3:+67914576 | None:intergenic |
| ATCATCAGATGTTAGAAAAT+GGG | 0.425313 | 2.3:-67913211 | MS.gene05808:CDS |
| TTAATTCTGACCATAGGAAA+TGG | 0.426007 | 2.3:-67913337 | MS.gene05808:CDS |
| TCACGCTTTGGGAAAATAGA+AGG | 0.429485 | 2.3:-67913455 | MS.gene05808:CDS |
| CGGCGTAAGAAAGGTCATTA+AGG | 0.433721 | 2.3:+67913495 | None:intergenic |
| TCAGCTGCAAAATTCTCAAC+TGG | 0.441332 | 2.3:-67913563 | MS.gene05808:CDS |
| ACTTCAGGTCTTTGCTTGAA+AGG | 0.470876 | 2.3:-67913428 | MS.gene05808:CDS |
| GAGCTAACACGGCGTAAGAA+AGG | 0.484665 | 2.3:+67913486 | None:intergenic |
| AGTTTCCTTGAAGCATATGC+TGG | 0.496553 | 2.3:-67913605 | MS.gene05808:CDS |
| TCCTTAACTACTGAAACCCT+TGG | 0.503695 | 2.3:-67914528 | MS.gene05808:CDS |
| GTCATCTCTACTATTCGTGC+CGG | 0.513748 | 2.3:+67913517 | None:intergenic |
| TGTAATCAGATCATATGGTA+TGG | 0.530296 | 2.3:-67913877 | MS.gene05808:intron |
| AGGAATTTACCATATTTCCA+TGG | 0.534844 | 2.3:+67914475 | None:intergenic |
| AGATGTTAGAAAATGGGAAA+GGG | 0.535212 | 2.3:-67913205 | MS.gene05808:CDS |
| AAGGCATCATCCATTTCCTA+TGG | 0.542944 | 2.3:+67913327 | None:intergenic |
| TCTATATTTAGAAGCATCCA+TGG | 0.553025 | 2.3:-67914492 | MS.gene05808:CDS |
| CAGATGTTAGAAAATGGGAA+AGG | 0.557087 | 2.3:-67913206 | MS.gene05808:CDS |
| ATATTATCTATCAACTCAGC+CGG | 0.573023 | 2.3:-67913536 | MS.gene05808:CDS |
| GGATACTTAATTCTGACCAT+AGG | 0.582839 | 2.3:-67913343 | MS.gene05808:CDS |
| AGAGCTTTGAACACAACCAA+GGG | 0.600437 | 2.3:+67914512 | None:intergenic |
| AGATCATATGGTATGGAGCA+TGG | 0.601542 | 2.3:-67913870 | MS.gene05808:CDS |
| AGAGGATATGGCATGTTCTG+TGG | 0.613331 | 2.3:-67914643 | None:intergenic |
| ACAGAAATCAATGTTAACCA+AGG | 0.624182 | 2.3:-67913657 | MS.gene05808:intron |
| TAGAGCTTTGAACACAACCA+AGG | 0.633504 | 2.3:+67914511 | None:intergenic |
| GTTAACCAAGGTATCAACCA+TGG | 0.651380 | 2.3:-67913645 | MS.gene05808:CDS |
| TATGCTGGAGAATCAAGAGA+TGG | 0.654209 | 2.3:-67913590 | MS.gene05808:CDS |
| TGACCTGAAAAGAGCTAACA+CGG | 0.676515 | 2.3:+67913475 | None:intergenic |
| TAGTTGTGTGCATTCATGTG+TGG | 0.691763 | 2.3:-67913391 | MS.gene05808:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAAATTGAATTGAAAAT+TGG | + | chr2.3:67913380-67913399 | None:intergenic | 15.0% |
| !! | CATATAATATCATCTTTATT+TGG | - | chr2.3:67913564-67913583 | MS.gene05808:CDS | 15.0% |
| !! | TGTGTTAGAATAAATTAAAA+TGG | + | chr2.3:67914115-67914134 | None:intergenic | 15.0% |
| !! | TTAGAATAAATTAAAATGGA+GGG | + | chr2.3:67914111-67914130 | None:intergenic | 15.0% |
| !! | GTTAGAATAAATTAAAATGG+AGG | + | chr2.3:67914112-67914131 | None:intergenic | 20.0% |
| !!! | ACAATTTTTAACTTCAAGTT+AGG | + | chr2.3:67913778-67913797 | None:intergenic | 20.0% |
| !!! | TGAAGACTAAAAAATGATTT+TGG | - | chr2.3:67913720-67913739 | MS.gene05808:intron | 20.0% |
| ! | ATCATCAGATGTTAGAAAAT+GGG | - | chr2.3:67914600-67914619 | MS.gene05808:CDS | 25.0% |
| ! | ATTATAGATGATCTTGTTCA+TGG | - | chr2.3:67913247-67913266 | MS.gene05808:CDS | 25.0% |
| ! | TAAATTCACAATGGAAGAAA+TGG | + | chr2.3:67913496-67913515 | None:intergenic | 25.0% |
| ! | TCATCTATAATCACAATTGT+TGG | + | chr2.3:67913238-67913257 | None:intergenic | 25.0% |
| ! | TTTCATGTAATCAGATCATA+TGG | - | chr2.3:67913929-67913948 | MS.gene05808:intron | 25.0% |
| !! | ATGTTCAATTAATTTTGCCA+TGG | + | chr2.3:67914186-67914205 | None:intergenic | 25.0% |
| !! | TCATGTTTGGATTCAATTTT+GGG | - | chr2.3:67913680-67913699 | MS.gene05808:intron | 25.0% |
| !! | TTCATGTTTGGATTCAATTT+TGG | - | chr2.3:67913679-67913698 | MS.gene05808:intron | 25.0% |
| !!! | AAATGATTTTGGTGTATGTT+TGG | - | chr2.3:67913731-67913750 | MS.gene05808:intron | 25.0% |
| ACAGAAATCAATGTTAACCA+AGG | - | chr2.3:67914154-67914173 | MS.gene05808:intron | 30.0% | |
| AGATGTTAGAAAATGGGAAA+GGG | - | chr2.3:67914606-67914625 | MS.gene05808:CDS | 30.0% | |
| AGGAATTTACCATATTTCCA+TGG | + | chr2.3:67913339-67913358 | None:intergenic | 30.0% | |
| ATATTATCTATCAACTCAGC+CGG | - | chr2.3:67914275-67914294 | MS.gene05808:intron | 30.0% | |
| ATGCATACAAAACATCTGAA+AGG | + | chr2.3:67914030-67914049 | None:intergenic | 30.0% | |
| ATGGAGGGAAATAATTGATT+TGG | + | chr2.3:67914096-67914115 | None:intergenic | 30.0% | |
| CAGCAAAGTTTCAAACAAAA+GGG | + | chr2.3:67913456-67913475 | None:intergenic | 30.0% | |
| CATCATCAGATGTTAGAAAA+TGG | - | chr2.3:67914599-67914618 | MS.gene05808:CDS | 30.0% | |
| TCAGCAAAGTTTCAAACAAA+AGG | + | chr2.3:67913457-67913476 | None:intergenic | 30.0% | |
| TGTAATCAGATCATATGGTA+TGG | - | chr2.3:67913934-67913953 | MS.gene05808:intron | 30.0% | |
| TTAATTCTGACCATAGGAAA+TGG | - | chr2.3:67914474-67914493 | MS.gene05808:intron | 30.0% | |
| TTCAAAGTAGTGTATGTTAG+TGG | - | chr2.3:67914545-67914564 | MS.gene05808:CDS | 30.0% | |
| TTTGCAGCATAAATTCACAA+TGG | + | chr2.3:67913505-67913524 | None:intergenic | 30.0% | |
| ! | ATTTATGCTGCAAATTTGCT+TGG | - | chr2.3:67913511-67913530 | MS.gene05808:CDS | 30.0% |
| ! | GGCTTTTAACAACAAGAATT+TGG | - | chr2.3:67913189-67913208 | MS.gene05808:CDS | 30.0% |
| !! | CTCTTCATTTTTGTTACTCA+TGG | - | chr2.3:67914447-67914466 | MS.gene05808:intron | 30.0% |
| !! | TCATCAAAAGTTTAGTTTGC+TGG | - | chr2.3:67913603-67913622 | MS.gene05808:CDS | 30.0% |
| !! | TCTATATTTAGAAGCATCCA+TGG | - | chr2.3:67913319-67913338 | MS.gene05808:CDS | 30.0% |
| !!! | ATCGCGAATTTTTCAAGATA+AGG | + | chr2.3:67914506-67914525 | None:intergenic | 30.0% |
| AATCCAAACATGAACTACCA+TGG | + | chr2.3:67913673-67913692 | None:intergenic | 35.0% | |
| ACATGAATGCACACAACTAT+TGG | + | chr2.3:67914419-67914438 | None:intergenic | 35.0% | |
| ATGGCAGAAGCAATCTATTA+AGG | - | chr2.3:67913960-67913979 | MS.gene05808:intron | 35.0% | |
| CAGATGTTAGAAAATGGGAA+AGG | - | chr2.3:67914605-67914624 | MS.gene05808:CDS | 35.0% | |
| GGAAATCAGAGAAAGAAAAC+AGG | + | chr2.3:67913359-67913378 | None:intergenic | 35.0% | |
| GGATACTTAATTCTGACCAT+AGG | - | chr2.3:67914468-67914487 | MS.gene05808:intron | 35.0% | |
| ! | AAAATAGAAGGCATGACTTC+AGG | - | chr2.3:67914368-67914387 | MS.gene05808:intron | 35.0% |
| !! | ACAATGCGTGTTTGTTTCTA+CGG | + | chr2.3:67913882-67913901 | None:intergenic | 35.0% |
| !! | TAGAAGCATCCATGGAAATA+TGG | - | chr2.3:67913327-67913346 | MS.gene05808:CDS | 35.0% |
| ACCAAGGGTTTCAGTAGTTA+AGG | + | chr2.3:67913287-67913306 | None:intergenic | 40.0% | |
| ACTTCAGGTCTTTGCTTGAA+AGG | - | chr2.3:67914383-67914402 | MS.gene05808:intron | 40.0% | |
| AGATCATATGGTATGGAGCA+TGG | - | chr2.3:67913941-67913960 | MS.gene05808:intron | 40.0% | |
| AGTTTCCTTGAAGCATATGC+TGG | - | chr2.3:67914206-67914225 | MS.gene05808:intron | 40.0% | |
| ATTCTCCAGCATATGCTTCA+AGG | + | chr2.3:67914214-67914233 | None:intergenic | 40.0% | |
| TAGTTGTGTGCATTCATGTG+TGG | - | chr2.3:67914420-67914439 | MS.gene05808:intron | 40.0% | |
| TATGCTGGAGAATCAAGAGA+TGG | - | chr2.3:67914221-67914240 | MS.gene05808:intron | 40.0% | |
| TCAGCTGCAAAATTCTCAAC+TGG | - | chr2.3:67914248-67914267 | MS.gene05808:intron | 40.0% | |
| TCCTTAACTACTGAAACCCT+TGG | - | chr2.3:67913283-67913302 | MS.gene05808:CDS | 40.0% | |
| TGACCTGAAAAGAGCTAACA+CGG | + | chr2.3:67914339-67914358 | None:intergenic | 40.0% | |
| ! | AAGGCATCATCCATTTCCTA+TGG | + | chr2.3:67914487-67914506 | None:intergenic | 40.0% |
| ! | CTGAAGTTGTCATTTGCTGA+AGG | + | chr2.3:67913639-67913658 | None:intergenic | 40.0% |
| ! | CTGCAAATTTGCTTGGAAGT+TGG | - | chr2.3:67913518-67913537 | MS.gene05808:CDS | 40.0% |
| ! | GTTAACCAAGGTATCAACCA+TGG | - | chr2.3:67914166-67914185 | MS.gene05808:intron | 40.0% |
| ! | TGTGTAAAGCTCACGTTTTG+AGG | + | chr2.3:67913809-67913828 | None:intergenic | 40.0% |
| !! | AGAGCTTTGAACACAACCAA+GGG | + | chr2.3:67913302-67913321 | None:intergenic | 40.0% |
| !! | TAGAGCTTTGAACACAACCA+AGG | + | chr2.3:67913303-67913322 | None:intergenic | 40.0% |
| !! | TCACGCTTTGGGAAAATAGA+AGG | - | chr2.3:67914356-67914375 | MS.gene05808:intron | 40.0% |
| !! | TTTTGCCATGGTTGATACCT+TGG | + | chr2.3:67914174-67914193 | None:intergenic | 40.0% |
| CATGAACTACCATGGAGCAA+TGG | + | chr2.3:67913665-67913684 | None:intergenic | 45.0% | |
| CGGCGTAAGAAAGGTCATTA+AGG | + | chr2.3:67914319-67914338 | None:intergenic | 45.0% | |
| GCTCCATGGTAGTTCATGTT+TGG | - | chr2.3:67913667-67913686 | MS.gene05808:intron | 45.0% | |
| GTCATCTCTACTATTCGTGC+CGG | + | chr2.3:67914297-67914316 | None:intergenic | 45.0% | |
| !!! | CTCTTTTCAGGTCACGCTTT+GGG | - | chr2.3:67914345-67914364 | MS.gene05808:intron | 45.0% |
| CTTCAGATGCCATTGCTCCA+TGG | - | chr2.3:67913653-67913672 | MS.gene05808:CDS | 50.0% | |
| GAGCTAACACGGCGTAAGAA+AGG | + | chr2.3:67914328-67914347 | None:intergenic | 50.0% | |
| !! | ACGCCGTGTTAGCTCTTTTC+AGG | - | chr2.3:67914333-67914352 | MS.gene05808:intron | 50.0% |
| !!! | GCTCTTTTCAGGTCACGCTT+TGG | - | chr2.3:67914344-67914363 | MS.gene05808:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 67913175 | 67914658 | 67913175 | ID=MS.gene05808 |
| chr2.3 | mRNA | 67913175 | 67914658 | 67913175 | ID=MS.gene05808.t1;Parent=MS.gene05808 |
| chr2.3 | exon | 67914485 | 67914658 | 67914485 | ID=MS.gene05808.t1.exon1;Parent=MS.gene05808.t1 |
| chr2.3 | CDS | 67914485 | 67914658 | 67914485 | ID=cds.MS.gene05808.t1;Parent=MS.gene05808.t1 |
| chr2.3 | exon | 67913825 | 67913890 | 67913825 | ID=MS.gene05808.t1.exon2;Parent=MS.gene05808.t1 |
| chr2.3 | CDS | 67913825 | 67913890 | 67913825 | ID=cds.MS.gene05808.t1;Parent=MS.gene05808.t1 |
| chr2.3 | exon | 67913175 | 67913675 | 67913175 | ID=MS.gene05808.t1.exon3;Parent=MS.gene05808.t1 |
| chr2.3 | CDS | 67913175 | 67913675 | 67913175 | ID=cds.MS.gene05808.t1;Parent=MS.gene05808.t1 |
| Gene Sequence |
| Protein sequence |
