AlfalfaGEDB | MS.gene43617

M. sativa cultivar XinJiangDaYe / MS.gene43617

KEGG: -
GO: -
PFAM: PF04520.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43617.t1	XP_013462645.1	72	132	32	1	1	132	1	127	1.50E-40	175.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43617.t1	A0A072VET2	72.0	132	32	1	1	132	1	127	1.1e-40	175.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049680	MS.gene43617	0.801165	1.02E-48	-1.69E-46
MS.gene055150	MS.gene43617	0.816667	4.91E-52	-1.69E-46
MS.gene055886	MS.gene43617	0.811226	7.78E-51	-1.69E-46
MS.gene059436	MS.gene43617	0.813297	2.75E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43617.t1	MTR_2g018175	95.455	132	1	1	1	132	1	127	1.18e-88	253
MS.gene43617.t1	MTR_2g023390	50.602	83	33	3	57	131	74	156	3.16e-16	70.9
MS.gene43617.t1	MTR_3g052330	61.224	49	17	1	85	131	150	198	1.35e-12	62.0
MS.gene43617.t1	MTR_2g094410	40.816	98	44	4	43	129	60	154	1.28e-11	58.9
MS.gene43617.t1	MTR_7g105130	56.250	48	19	1	86	131	170	217	9.12e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43617.t1	AT1G29640	40.000	145	69	6	1	132	1	140	6.63e-25	92.8
MS.gene43617.t1	AT2G34340	39.860	143	68	5	1	132	1	136	1.35e-23	89.4
MS.gene43617.t1	AT5G45630	34.532	139	59	4	1	132	1	114	7.85e-20	79.0
MS.gene43617.t1	AT4G21970	46.667	90	29	3	57	131	60	145	2.10e-18	76.6
MS.gene43617.t1	AT4G18980	42.222	135	46	7	1	132	1	106	5.89e-18	73.9
MS.gene43617.t1	AT4G04630	64.000	50	16	1	84	131	119	168	5.40e-15	67.8
MS.gene43617.t1	AT3G15040	56.863	51	20	1	83	131	193	243	9.26e-12	60.5

Find 46 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTTAGGAATTCGATTCTT+AGG	0.266852	2.2:-66443316	MS.gene43617:CDS
CAGCTTCAGATTGATGAATC+TGG	0.295768	2.2:+66443627	None:intergenic
CTAGCCTCCGCGCCATAATC+AGG	0.322627	2.2:+66443405	None:intergenic
GAAGGGATTTGAGCAGGGTT+AGG	0.370718	2.2:-66443331	MS.gene43617:CDS
GGTGCCGCCGCACCTGATTA+TGG	0.386353	2.2:-66443417	MS.gene43617:CDS
TGAAGGGAAGGGATTTGAGC+AGG	0.444324	2.2:-66443337	MS.gene43617:CDS
ATTATGGCGCGGAGGCTAGC+TGG	0.455420	2.2:-66443401	MS.gene43617:CDS
ATATTTCACCGGAGGGGTAA+TGG	0.465755	2.2:-66443470	MS.gene43617:CDS
AATTCGATTCTTAGGATGAC+TGG	0.471954	2.2:-66443308	MS.gene43617:CDS
CCATGTGTTCTGGGCATGGA+AGG	0.480561	2.2:-66443364	MS.gene43617:CDS
TGGGCATGGAAGGACTTTGA+AGG	0.498075	2.2:-66443354	MS.gene43617:CDS
GGTGCTGCTGAAGCTGAAGA+AGG	0.521781	2.2:-66443594	MS.gene43617:CDS
CTATGAGGAGGACGAGGATG+AGG	0.526881	2.2:-66443447	MS.gene43617:CDS
CCGGAGGGGTAATGGCTATG+AGG	0.530842	2.2:-66443462	MS.gene43617:CDS
GGACGAGGATGAGGAGGAGA+TGG	0.533776	2.2:-66443438	MS.gene43617:CDS
CCTCATAGCCATTACCCCTC+CGG	0.534896	2.2:+66443462	None:intergenic
CCCTCTGAGGGGATATTCAT+CGG	0.539053	2.2:+66443494	None:intergenic
CCTTCCATGCCCAGAACACA+TGG	0.539401	2.2:+66443364	None:intergenic
TGGAAGGACTTTGAAGGGAA+GGG	0.555164	2.2:-66443348	MS.gene43617:CDS
CAACAACAACAAGAAGAAGA+TGG	0.556088	2.2:-66443528	MS.gene43617:CDS
CCTCAGAGGGGATATTTCAC+CGG	0.559919	2.2:-66443481	MS.gene43617:CDS
TGAGGAGTTTCTTGAATCCG+AGG	0.568990	2.2:-66443663	MS.gene43617:CDS
ATCTGAAGCTGAAGACAAGA+AGG	0.569372	2.2:-66443615	MS.gene43617:CDS
GGGCATGGAAGGACTTTGAA+GGG	0.570682	2.2:-66443353	MS.gene43617:CDS
TGAGGGGATATTCATCGGCA+TGG	0.573297	2.2:+66443499	None:intergenic
ATGGAAGGACTTTGAAGGGA+AGG	0.573855	2.2:-66443349	MS.gene43617:CDS
CGGTGAAATATCCCCTCTGA+GGG	0.581904	2.2:+66443482	None:intergenic
CCGATGAATATCCCCTCAGA+GGG	0.585354	2.2:-66443494	MS.gene43617:CDS
GAAGGGAAGGGATTTGAGCA+GGG	0.588262	2.2:-66443336	MS.gene43617:CDS
TAATGGCTATGAGGAGGACG+AGG	0.588381	2.2:-66443453	MS.gene43617:CDS
TAACTTCACCAACATGTCTG+AGG	0.596960	2.2:-66443681	None:intergenic
TGAGGAGGACGAGGATGAGG+AGG	0.598626	2.2:-66443444	MS.gene43617:CDS
CGCGCCATAATCAGGTGCGG+CGG	0.605054	2.2:+66443413	None:intergenic
CGCCGCACCTGATTATGGCG+CGG	0.605485	2.2:-66443412	MS.gene43617:CDS
GCTGCTGAAGCTGAAGAAGG+AGG	0.612278	2.2:-66443591	MS.gene43617:CDS
CTCCGCGCCATAATCAGGTG+CGG	0.621040	2.2:+66443410	None:intergenic
CCGGTGAAATATCCCCTCTG+AGG	0.632955	2.2:+66443481	None:intergenic
AGAGGGGATATTTCACCGGA+GGG	0.643919	2.2:-66443477	MS.gene43617:CDS
CAGAGGGGATATTTCACCGG+AGG	0.645698	2.2:-66443478	MS.gene43617:CDS
CGCACCTGATTATGGCGCGG+AGG	0.650193	2.2:-66443409	MS.gene43617:CDS
GCCGATGAATATCCCCTCAG+AGG	0.662649	2.2:-66443495	MS.gene43617:CDS
GAGGGGTAATGGCTATGAGG+AGG	0.676604	2.2:-66443459	MS.gene43617:CDS
ACAACAAGAAGAAGATGGTG+AGG	0.679378	2.2:-66443523	MS.gene43617:CDS
GAGGGGATATTTCACCGGAG+GGG	0.727432	2.2:-66443476	MS.gene43617:CDS
CGATGAATATCCCCTCAGAG+GGG	0.754143	2.2:-66443493	MS.gene43617:CDS
GGTGAAATATCCCCTCTGAG+GGG	0.755545	2.2:+66443483	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTCGATTCTTAGGATGAC+TGG	-	chr2.2:66443652-66443671	MS.gene43617:CDS	35.0%
	CAACAACAACAAGAAGAAGA+TGG	-	chr2.2:66443432-66443451	MS.gene43617:CDS	35.0%
	CTGGTTGAAAAAAAGAACCT+CGG	+	chr2.2:66443317-66443336	None:intergenic	35.0%
!	ATGACATTTTCCATGTGTTC+TGG	-	chr2.2:66443586-66443605	MS.gene43617:CDS	35.0%
!	TGACATTTTCCATGTGTTCT+GGG	-	chr2.2:66443587-66443606	MS.gene43617:CDS	35.0%
!!!	TAGGATGACTGGTTTTATTG+AGG	-	chr2.2:66443663-66443682	MS.gene43617:CDS	35.0%
	ACAACAAGAAGAAGATGGTG+AGG	-	chr2.2:66443437-66443456	MS.gene43617:CDS	40.0%
	CAGCTTCAGATTGATGAATC+TGG	+	chr2.2:66443336-66443355	None:intergenic	40.0%
	GGGTTAGGAATTCGATTCTT+AGG	-	chr2.2:66443644-66443663	MS.gene43617:CDS	40.0%
!	ATCTGAAGCTGAAGACAAGA+AGG	-	chr2.2:66443345-66443364	MS.gene43617:CDS	40.0%
	ATATTTCACCGGAGGGGTAA+TGG	-	chr2.2:66443490-66443509	MS.gene43617:CDS	45.0%
	TGAGGAGTTTCTTGAATCCG+AGG	-	chr2.2:66443297-66443316	MS.gene43617:CDS	45.0%
!	ATGGAAGGACTTTGAAGGGA+AGG	-	chr2.2:66443611-66443630	MS.gene43617:CDS	45.0%
!	TGGAAGGACTTTGAAGGGAA+GGG	-	chr2.2:66443612-66443631	MS.gene43617:CDS	45.0%
!!	TTTTCCATGTGTTCTGGGCA+TGG	-	chr2.2:66443592-66443611	MS.gene43617:CDS	45.0%
	AGAGGGGATATTTCACCGGA+GGG	-	chr2.2:66443483-66443502	MS.gene43617:CDS	50.0%
	CCGATGAATATCCCCTCAGA+GGG	-	chr2.2:66443466-66443485	MS.gene43617:CDS	50.0%
	CCTCAGAGGGGATATTTCAC+CGG	-	chr2.2:66443479-66443498	MS.gene43617:CDS	50.0%
	CGATGAATATCCCCTCAGAG+GGG	-	chr2.2:66443467-66443486	MS.gene43617:CDS	50.0%
	CGGTGAAATATCCCCTCTGA+GGG	+	chr2.2:66443481-66443500	None:intergenic	50.0%
	GAAGGGAAGGGATTTGAGCA+GGG	-	chr2.2:66443624-66443643	MS.gene43617:CDS	50.0%
	GAAGGGATTTGAGCAGGGTT+AGG	-	chr2.2:66443629-66443648	MS.gene43617:CDS	50.0%
	GGGCATGGAAGGACTTTGAA+GGG	-	chr2.2:66443607-66443626	MS.gene43617:CDS	50.0%
	GGTGAAATATCCCCTCTGAG+GGG	+	chr2.2:66443480-66443499	None:intergenic	50.0%
	TAATGGCTATGAGGAGGACG+AGG	-	chr2.2:66443507-66443526	MS.gene43617:CDS	50.0%
	TGAAGGGAAGGGATTTGAGC+AGG	-	chr2.2:66443623-66443642	MS.gene43617:CDS	50.0%
	TGAGGGGATATTCATCGGCA+TGG	+	chr2.2:66443464-66443483	None:intergenic	50.0%
	TGGGCATGGAAGGACTTTGA+AGG	-	chr2.2:66443606-66443625	MS.gene43617:CDS	50.0%
!	CCCTCTGAGGGGATATTCAT+CGG	+	chr2.2:66443469-66443488	None:intergenic	50.0%
	CAGAGGGGATATTTCACCGG+AGG	-	chr2.2:66443482-66443501	MS.gene43617:CDS	55.0%
	CCGGTGAAATATCCCCTCTG+AGG	+	chr2.2:66443482-66443501	None:intergenic	55.0%
	CCTCATAGCCATTACCCCTC+CGG	+	chr2.2:66443501-66443520	None:intergenic	55.0%
	CCTTCCATGCCCAGAACACA+TGG	+	chr2.2:66443599-66443618	None:intergenic	55.0%
	CTATGAGGAGGACGAGGATG+AGG	-	chr2.2:66443513-66443532	MS.gene43617:CDS	55.0%
	GAGGGGATATTTCACCGGAG+GGG	-	chr2.2:66443484-66443503	MS.gene43617:CDS	55.0%
	GCCGATGAATATCCCCTCAG+AGG	-	chr2.2:66443465-66443484	MS.gene43617:CDS	55.0%
	GCTGCTGAAGCTGAAGAAGG+AGG	-	chr2.2:66443369-66443388	MS.gene43617:CDS	55.0%
!	CCATGTGTTCTGGGCATGGA+AGG	-	chr2.2:66443596-66443615	MS.gene43617:CDS	55.0%
!	GAGGGGTAATGGCTATGAGG+AGG	-	chr2.2:66443501-66443520	MS.gene43617:CDS	55.0%
!!	GGTGCTGCTGAAGCTGAAGA+AGG	-	chr2.2:66443366-66443385	MS.gene43617:CDS	55.0%
	ATTATGGCGCGGAGGCTAGC+TGG	-	chr2.2:66443559-66443578	MS.gene43617:CDS	60.0%
	CTAGCCTCCGCGCCATAATC+AGG	+	chr2.2:66443558-66443577	None:intergenic	60.0%
	CTCCGCGCCATAATCAGGTG+CGG	+	chr2.2:66443553-66443572	None:intergenic	60.0%
	GGACGAGGATGAGGAGGAGA+TGG	-	chr2.2:66443522-66443541	MS.gene43617:CDS	60.0%
	TGAGGAGGACGAGGATGAGG+AGG	-	chr2.2:66443516-66443535	MS.gene43617:CDS	60.0%
!	CCGGAGGGGTAATGGCTATG+AGG	-	chr2.2:66443498-66443517	MS.gene43617:CDS	60.0%
	CGCACCTGATTATGGCGCGG+AGG	-	chr2.2:66443551-66443570	MS.gene43617:CDS	65.0%
	CGCCGCACCTGATTATGGCG+CGG	-	chr2.2:66443548-66443567	MS.gene43617:CDS	65.0%
	CGCGCCATAATCAGGTGCGG+CGG	+	chr2.2:66443550-66443569	None:intergenic	65.0%
!	GGTGCCGCCGCACCTGATTA+TGG	-	chr2.2:66443543-66443562	MS.gene43617:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	66443292	66443690	66443292	ID=MS.gene43617
chr2.2	mRNA	66443292	66443690	66443292	ID=MS.gene43617.t1;Parent=MS.gene43617
chr2.2	exon	66443292	66443690	66443292	ID=MS.gene43617.t1.exon1;Parent=MS.gene43617.t1
chr2.2	CDS	66443292	66443690	66443292	ID=cds.MS.gene43617.t1;Parent=MS.gene43617.t1

Gene Sequence

>MS.gene43617

ATGTCTGAGGAGTTTCTTGAATCCGAGGTTCTTTTTTTCAACCAGATTCATCAATCTGAAGCTGAAGACAAGAAGGTGCTGCTGAAGCTGAAGAAGGAGGAGATCAATTCTGATGATGAGATTGATCAAGCTGCTGACAACAACAACAACAAGAAGAAGATGGTGAGGTCCATGCCGATGAATATCCCCTCAGAGGGGATATTTCACCGGAGGGGTAATGGCTATGAGGAGGACGAGGATGAGGAGGAGATGGTGCCGCCGCACCTGATTATGGCGCGGAGGCTAGCTGGAAAAATGACATTTTCCATGTGTTCTGGGCATGGAAGGACTTTGAAGGGAAGGGATTTGAGCAGGGTTAGGAATTCGATTCTTAGGATGACTGGTTTTATTGAGGTCTGA

Protein sequence

>MS.gene43617.t1

MSEEFLESEVLFFNQIHQSEAEDKKVLLKLKKEEINSDDEIDQAADNNNNKKKMVRSMPMNIPSEGIFHRRGNGYEEDEDEEEMVPPHLIMARRLAGKMTFSMCSGHGRTLKGRDLSRVRNSILRMTGFIEV