AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44166

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44166.t1 MTR_1g083750 97.400 423 11 0 1 423 1 423 0.0 846
MS.gene44166.t1 MTR_7g112430 85.071 422 61 2 1 422 1 420 0.0 717
MS.gene44166.t1 MTR_1g083750 96.979 331 10 0 93 423 1 331 0.0 658
MS.gene44166.t1 MTR_1g110210 73.065 323 85 1 1 323 1 321 5.17e-173 487
MS.gene44166.t1 MTR_7g029240 62.673 434 137 13 1 422 1 421 1.34e-167 477
MS.gene44166.t1 MTR_1g019760 56.098 410 178 2 14 423 11 418 4.63e-167 476
MS.gene44166.t1 MTR_3g101540 55.556 414 175 3 14 422 10 419 4.08e-163 466
MS.gene44166.t1 MTR_3g101540 55.556 414 175 3 14 422 10 419 5.13e-163 466
MS.gene44166.t1 MTR_1g019760 58.671 346 141 2 14 359 11 354 5.08e-148 427
MS.gene44166.t1 MTR_1g014640 31.449 283 155 12 25 298 72 324 2.14e-28 114
MS.gene44166.t1 MTR_1g013400 31.449 283 155 12 25 298 72 324 2.14e-28 114
MS.gene44166.t1 MTR_3g105730 31.452 248 144 9 57 298 32 259 3.09e-27 110
MS.gene44166.t1 MTR_3g105730 30.924 249 144 9 57 298 54 281 3.87e-27 110
MS.gene44166.t1 MTR_1g115570 31.673 281 155 14 25 299 90 339 1.72e-25 106
MS.gene44166.t1 MTR_3g464650 29.779 272 163 11 33 297 64 314 5.14e-25 104
MS.gene44166.t1 MTR_3g464700 31.579 228 136 8 33 260 88 295 2.20e-24 103
MS.gene44166.t1 MTR_5g071550 28.625 269 164 11 36 297 127 374 2.44e-22 98.2
MS.gene44166.t1 MTR_8g463130 30.043 233 139 8 68 295 66 279 3.58e-21 93.2
MS.gene44166.t1 MTR_8g463130 30.043 233 139 8 68 295 38 251 4.45e-21 92.4
MS.gene44166.t1 MTR_2g078760 29.572 257 152 11 46 297 21 253 3.86e-20 89.7
MS.gene44166.t1 MTR_2g078760 29.572 257 152 11 46 297 100 332 4.14e-20 91.3
MS.gene44166.t1 MTR_4g125810 27.365 296 159 10 46 297 220 503 7.72e-20 92.0
MS.gene44166.t1 MTR_3g091060 29.020 255 152 9 46 295 108 338 2.88e-19 89.0
MS.gene44166.t1 MTR_4g094208 27.698 278 168 10 27 295 21 274 5.66e-19 87.0
MS.gene44166.t1 MTR_1g050520 31.301 246 136 7 69 299 419 646 6.49e-19 89.7
MS.gene44166.t1 MTR_5g071550 28.636 220 137 8 36 255 127 326 9.51e-19 87.4
MS.gene44166.t1 MTR_4g123080 29.699 266 151 10 67 313 94 342 1.09e-18 87.4
MS.gene44166.t1 MTR_4g123080 30.556 252 139 10 67 299 112 346 1.47e-18 87.0
MS.gene44166.t1 MTR_5g024340 26.894 264 153 9 67 314 123 362 2.04e-18 86.7
MS.gene44166.t1 MTR_3g074610 29.032 279 163 11 27 295 26 279 3.77e-18 84.7
MS.gene44166.t1 MTR_2g008850 25.818 275 177 9 27 295 25 278 4.81e-18 84.3
MS.gene44166.t1 MTR_2g008850 27.778 234 143 9 68 295 19 232 7.02e-18 82.8
MS.gene44166.t1 MTR_5g063940 29.137 278 164 12 27 295 26 279 1.23e-17 83.2
MS.gene44166.t1 MTR_5g063940 30.472 233 138 9 68 295 19 232 1.94e-17 81.6
MS.gene44166.t1 MTR_5g009370 29.524 210 123 7 66 265 85 279 2.20e-17 83.2
MS.gene44166.t1 MTR_1g015110 30.712 267 137 10 69 299 291 545 1.32e-16 82.4
MS.gene44166.t1 MTR_4g094542 29.921 254 150 10 46 295 133 362 2.15e-16 80.5
MS.gene44166.t1 MTR_3g068200 29.032 217 122 6 62 261 117 318 2.23e-16 80.9
MS.gene44166.t1 MTR_2g078760 30.332 211 120 9 46 252 100 287 2.65e-16 79.3
MS.gene44166.t1 MTR_2g040500 25.857 321 162 10 40 297 228 535 2.83e-16 81.3
MS.gene44166.t1 MTR_4g116420 29.762 252 132 10 65 295 121 348 5.44e-16 79.7
MS.gene44166.t1 MTR_1g086350 28.358 268 156 11 66 314 14 264 6.75e-16 78.2
MS.gene44166.t1 MTR_8g102550 29.442 197 118 6 69 258 153 335 7.03e-16 79.3
MS.gene44166.t1 MTR_2g033000 31.373 255 131 11 64 295 96 329 7.24e-16 79.0
MS.gene44166.t1 MTR_6g081850 28.053 303 165 13 26 295 34 316 8.47e-16 78.2
MS.gene44166.t1 MTR_3g491830 24.633 341 185 14 29 322 54 369 1.11e-15 78.6
MS.gene44166.t1 MTR_3g104710 27.574 272 149 8 62 297 280 539 1.35e-15 79.3
MS.gene44166.t1 MTR_1g086350 28.464 267 155 11 67 314 101 350 1.79e-15 77.8
MS.gene44166.t1 MTR_1g086350 28.464 267 155 11 67 314 117 366 2.34e-15 77.8
MS.gene44166.t1 MTR_5g042243 85.366 41 6 0 1 41 1 41 3.19e-15 72.0
MS.gene44166.t1 MTR_7g112490 33.898 177 95 9 116 276 183 353 9.80e-15 75.9
MS.gene44166.t1 MTR_7g112490 33.898 177 95 9 116 276 165 335 1.38e-14 75.1
MS.gene44166.t1 MTR_1g028300 31.048 248 138 9 69 297 146 379 1.45e-14 75.1
MS.gene44166.t1 MTR_7g021530 30.403 273 142 14 36 283 48 297 2.98e-14 73.9
MS.gene44166.t1 MTR_5g080680 28.019 207 123 7 69 262 133 326 3.43e-14 74.3
MS.gene44166.t1 MTR_7g021530 30.403 273 142 14 36 283 98 347 7.11e-14 73.2
MS.gene44166.t1 MTR_7g021530 30.403 273 142 14 36 283 139 388 1.29e-13 72.8
MS.gene44166.t1 MTR_6g087000 27.517 298 167 14 36 297 97 381 2.28e-13 72.8
MS.gene44166.t1 MTR_6g087000 27.517 298 167 14 36 297 97 381 2.30e-13 72.8
MS.gene44166.t1 MTR_3g107880 27.969 261 145 9 67 308 120 356 2.65e-13 71.2
MS.gene44166.t1 MTR_3g107880 27.969 261 145 9 67 308 119 355 2.76e-13 71.2
MS.gene44166.t1 MTR_3g107880 27.969 261 145 9 67 308 62 298 3.25e-13 70.5
MS.gene44166.t1 MTR_4g118340 31.126 302 148 19 36 297 59 340 6.45e-13 70.5
MS.gene44166.t1 MTR_8g017240 29.949 197 113 9 100 283 136 320 6.65e-13 70.1
MS.gene44166.t1 MTR_2g020970 26.829 287 166 11 43 297 67 341 2.33e-12 68.2
MS.gene44166.t1 MTR_4g007440 34.043 141 66 5 128 257 156 280 4.83e-12 67.4
MS.gene44166.t1 MTR_2g020970 26.829 287 166 11 43 297 67 341 6.28e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44166.t1 AT1G09160 75.355 422 102 1 1 422 1 420 0.0 647
MS.gene44166.t1 AT1G09160 75.355 422 102 1 1 422 1 420 0.0 647
MS.gene44166.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene44166.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene44166.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene44166.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene44166.t1 AT1G47380 56.341 410 178 1 14 423 10 418 4.95e-170 483
MS.gene44166.t1 AT5G10740 30.921 304 168 13 6 298 8 280 4.25e-27 111
MS.gene44166.t1 AT5G10740 30.921 304 168 13 6 298 8 280 4.25e-27 111
MS.gene44166.t1 AT2G34740 33.054 239 136 8 65 298 116 335 7.91e-27 110
MS.gene44166.t1 AT1G07160 30.686 277 157 11 33 297 123 376 1.27e-25 107
MS.gene44166.t1 AT1G67820 32.787 244 139 10 34 270 78 303 1.44e-25 108
MS.gene44166.t1 AT5G24940 30.877 285 154 13 25 298 28 280 1.76e-25 108
MS.gene44166.t1 AT1G67820 32.787 244 139 10 34 270 114 339 1.89e-25 108
MS.gene44166.t1 AT4G31750 30.108 279 158 11 25 295 28 277 3.23e-25 105
MS.gene44166.t1 AT4G31750 30.357 280 156 12 25 295 28 277 3.88e-25 105
MS.gene44166.t1 AT4G31750 30.357 280 156 12 25 295 28 277 3.88e-25 105
MS.gene44166.t1 AT1G43900 31.970 269 148 14 36 295 124 366 3.61e-24 103
MS.gene44166.t1 AT1G22280 30.901 233 143 7 68 295 65 284 4.74e-24 101
MS.gene44166.t1 AT2G40180 28.269 283 183 8 21 297 117 385 9.74e-24 102
MS.gene44166.t1 AT1G34750 29.749 279 161 11 27 295 26 279 1.58e-23 100
MS.gene44166.t1 AT1G34750 29.749 279 161 11 27 295 26 279 1.58e-23 100
MS.gene44166.t1 AT1G34750 29.749 279 161 11 27 295 26 279 1.58e-23 100
MS.gene44166.t1 AT1G78200 30.357 280 161 11 27 295 24 280 1.97e-23 99.8
MS.gene44166.t1 AT1G78200 30.357 280 161 11 27 295 24 280 1.97e-23 99.8
MS.gene44166.t1 AT1G78200 30.357 280 161 11 27 295 24 280 1.97e-23 99.8
MS.gene44166.t1 AT1G78200 31.128 257 147 10 46 295 2 235 2.20e-23 98.6
MS.gene44166.t1 AT1G78200 31.128 257 147 10 46 295 2 235 2.20e-23 98.6
MS.gene44166.t1 AT4G28400 29.258 229 146 6 68 295 45 258 8.37e-23 97.4
MS.gene44166.t1 AT2G30020 28.782 271 163 11 36 297 143 392 1.07e-22 99.8
MS.gene44166.t1 AT5G53140 29.412 255 151 10 46 295 115 345 1.14e-22 99.8
MS.gene44166.t1 AT5G53140 29.412 255 151 10 46 295 115 345 1.14e-22 99.8
MS.gene44166.t1 AT4G28400 29.258 229 146 6 68 295 67 280 1.44e-22 97.4
MS.gene44166.t1 AT1G22280 30.901 233 137 8 68 295 65 278 3.40e-22 96.3
MS.gene44166.t1 AT2G20630 29.104 268 158 12 33 295 36 276 5.52e-22 95.5
MS.gene44166.t1 AT4G26080 27.737 274 149 7 61 299 165 424 1.33e-21 96.7
MS.gene44166.t1 AT3G51470 28.475 295 169 11 36 314 32 300 2.00e-21 94.7
MS.gene44166.t1 AT2G20630 28.839 267 158 12 33 294 36 275 2.17e-21 94.0
MS.gene44166.t1 AT3G51470 28.475 295 169 11 36 314 76 344 2.33e-21 95.1
MS.gene44166.t1 AT5G57050 28.464 267 145 9 65 297 117 371 3.87e-20 92.0
MS.gene44166.t1 AT5G57050 28.464 267 145 9 65 297 157 411 4.97e-20 92.0
MS.gene44166.t1 AT2G40860 29.286 280 157 9 36 299 396 650 1.47e-19 91.7
MS.gene44166.t1 AT2G25620 30.451 266 136 10 67 308 127 367 1.48e-19 90.5
MS.gene44166.t1 AT2G25620 30.451 266 136 10 67 308 127 367 1.48e-19 90.5
MS.gene44166.t1 AT2G40860 29.286 280 157 9 36 299 298 552 2.46e-19 90.9
MS.gene44166.t1 AT2G34740 29.665 209 127 5 65 270 112 303 2.68e-19 88.6
MS.gene44166.t1 AT2G34740 29.665 209 127 5 65 270 130 321 3.17e-19 88.6
MS.gene44166.t1 AT3G62260 29.151 271 157 12 67 319 26 279 1.14e-17 83.6
MS.gene44166.t1 AT2G40860 28.409 264 148 8 36 282 396 635 1.20e-17 85.9
MS.gene44166.t1 AT1G48040 32.692 260 129 12 63 295 107 347 2.08e-17 84.0
MS.gene44166.t1 AT3G62260 29.151 271 157 12 67 319 116 369 4.77e-17 82.8
MS.gene44166.t1 AT3G62260 29.151 271 157 12 67 319 115 368 5.06e-17 82.8
MS.gene44166.t1 AT3G15260 29.185 233 141 8 68 295 73 286 5.35e-17 81.3
MS.gene44166.t1 AT3G15260 29.185 233 141 8 68 295 73 286 5.35e-17 81.3
MS.gene44166.t1 AT1G34750 28.085 235 136 9 27 252 26 236 1.26e-16 79.3
MS.gene44166.t1 AT5G59220 28.287 251 150 10 31 265 107 343 2.92e-15 77.8
MS.gene44166.t1 AT4G28400 27.568 185 120 4 68 252 67 237 4.41e-15 75.1
MS.gene44166.t1 AT1G18030 29.020 255 135 10 69 295 110 346 5.41e-15 76.3
MS.gene44166.t1 AT1G07430 28.054 221 126 7 62 265 151 355 4.92e-14 73.9
MS.gene44166.t1 AT4G27800 24.848 330 173 12 40 322 67 368 1.37e-13 72.4
MS.gene44166.t1 AT3G11410 25.446 224 126 7 62 260 131 338 2.28e-12 68.6
MS.gene44166.t1 AT2G40860 29.114 237 127 8 36 255 396 608 3.20e-12 68.9
MS.gene44166.t1 AT1G16220 23.864 352 195 13 5 301 45 378 3.92e-12 68.2
MS.gene44166.t1 AT3G17250 28.063 253 140 13 67 295 157 391 4.18e-12 68.2
MS.gene44166.t1 AT4G27800 26.119 268 134 9 40 265 67 312 4.30e-12 67.4
MS.gene44166.t1 AT4G27800 26.119 268 134 9 40 265 67 312 4.34e-12 67.4
MS.gene44166.t1 AT1G79630 29.798 198 121 6 131 314 151 344 7.89e-12 67.0
MS.gene44166.t1 AT1G79630 29.798 198 121 6 131 314 151 344 7.89e-12 67.0
MS.gene44166.t1 AT5G27930 33.544 158 88 7 105 251 150 301 9.00e-12 66.2
MS.gene44166.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene44166.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene44166.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene44166.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene44166.t1 AT1G79630 29.500 200 123 6 131 316 217 412 1.05e-11 67.0
MS.gene44166.t1 AT1G17550 27.434 226 120 8 69 265 238 448 1.13e-11 67.0
MS.gene44166.t1 AT1G17550 27.434 226 120 8 69 265 238 448 1.13e-11 67.0
MS.gene44166.t1 AT1G79630 29.500 200 123 6 131 316 221 416 1.38e-11 66.6
MS.gene44166.t1 AT4G32950 30.114 176 110 5 131 297 138 309 4.10e-11 64.3
MS.gene44166.t1 AT5G51760 25.301 249 152 6 40 265 86 323 4.21e-11 64.7
MS.gene44166.t1 AT2G29380 27.928 222 132 8 59 265 103 311 4.50e-11 64.3
MS.gene44166.t1 AT1G18030 30.288 208 104 9 69 253 110 299 4.81e-11 63.9
MS.gene44166.t1 AT5G51760 25.301 249 152 6 40 265 116 353 5.20e-11 64.7
MS.gene44166.t1 AT5G36250 26.222 225 136 9 100 300 171 389 5.33e-11 64.7
MS.gene44166.t1 AT1G72770 25.090 279 143 11 69 297 239 501 5.48e-11 64.7
MS.gene44166.t1 AT1G72770 25.090 279 143 11 69 297 239 501 5.48e-11 64.7
MS.gene44166.t1 AT1G72770 25.090 279 143 11 69 297 239 501 5.48e-11 64.7
MS.gene44166.t1 AT2G25070 31.206 141 70 5 128 257 156 280 8.87e-11 63.5
MS.gene44166.t1 AT2G25070 31.206 141 70 5 128 257 156 280 8.87e-11 63.5

Find 118 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGCTTCTGATGGAATT+TGG 0.123861 1.3:-59303423 MS.gene44166:CDS
AGCACATTATGTTTCTTTCT+TGG 0.197447 1.3:+59303129 None:intergenic
GTTCCTTCCAATACAAATTC+TGG 0.255485 1.3:-59302494 MS.gene44166:CDS
TGTTTCTTTCTTGGAGTTGC+TGG 0.268275 1.3:+59303138 None:intergenic
GTGCTTAGTTCACTTCTCTT+TGG 0.288508 1.3:-59303111 MS.gene44166:CDS
TTACCTGCAGGGGAAACTTC+TGG 0.307065 1.3:-59303966 MS.gene44166:intron
TATAGCTTCTGATGGAATTT+GGG 0.324404 1.3:-59303422 MS.gene44166:CDS
GCTACGTTTGTGATTATTGA+TGG 0.331297 1.3:-59303936 MS.gene44166:CDS
CCGTTACAGTTGCATCTGTT+GGG 0.351128 1.3:-59303908 MS.gene44166:CDS
ACAGATTGCCATAGAGTTCC+TGG 0.368715 1.3:-59304586 MS.gene44166:CDS
ACTTGCTTAACATGTGGTAT+TGG 0.372029 1.3:+59303579 None:intergenic
TGCTGCCAAGCTGGTGGTTA+AGG 0.391611 1.3:-59303344 MS.gene44166:intron
AAGACGCCATGGAAGGAAAA+AGG 0.416517 1.3:-59302337 MS.gene44166:CDS
CAGATCCTTGACGGGCATAA+TGG 0.417208 1.3:-59304214 MS.gene44166:CDS
GGGCCTCTTCGCTGTTGGCC+TGG 0.420476 1.3:-59303657 MS.gene44166:CDS
AACCTGAACAGGAAGCTCTA+AGG 0.420809 1.3:-59303236 MS.gene44166:intron
GTAGGAAGACTCAACGTATT+CGG 0.426631 1.3:-59303771 MS.gene44166:CDS
GTTTCCTAGCTTTCAAATGC+TGG 0.427993 1.3:-59303457 MS.gene44166:intron
CTTGATTGGCAGATCCTTGA+CGG 0.435403 1.3:-59304223 MS.gene44166:intron
TGGAAGAAAATGTAGAAGAG+AGG 0.437691 1.3:-59303821 MS.gene44166:CDS
GTGAATACCAGAATTTGTAT+TGG 0.439513 1.3:+59302487 None:intergenic
CTTAAAATGCAGGCTAGGTA+AGG 0.443855 1.3:-59302519 MS.gene44166:intron
ATGGCTTCAAGCACTGCCTC+GGG 0.448779 1.3:-59304110 MS.gene44166:CDS
ACTAATAAGCTTTCTGCAGT+TGG 0.455037 1.3:-59303057 MS.gene44166:CDS
TCGTAGCTCACGGCCGATCA+AGG 0.456909 1.3:+59304690 None:intergenic
GTTGCTGGCATCTGCAACAC+AGG 0.461352 1.3:+59303153 None:intergenic
GGATACTTTGTCTTCTGATA+CGG 0.461894 1.3:-59303401 MS.gene44166:CDS
AATGGCTTCAAGCACTGCCT+CGG 0.462446 1.3:-59304111 MS.gene44166:CDS
ATCGGCCGTGAGCTACGAAA+TGG 0.463670 1.3:-59304685 MS.gene44166:CDS
GTTCCGTTGGCAACCTTGAT+CGG 0.465033 1.3:-59304703 MS.gene44166:CDS
CCATTCCGCAAGGTGTAAGC+AGG 0.465666 1.3:-59304138 MS.gene44166:CDS
AAGAATCGCCAGGAACTCTA+TGG 0.471757 1.3:+59304578 None:intergenic
GCCTGTCCATACTTCACAAA+AGG 0.476504 1.3:+59304649 None:intergenic
GAAGTATGGACAGGCTGGTT+TGG 0.479502 1.3:-59304641 MS.gene44166:CDS
GTGGAGGAGTTATTTGAAGA+GGG 0.481037 1.3:-59303030 MS.gene44166:CDS
CTGGGTATCTAATATGCATC+GGG 0.481436 1.3:+59303881 None:intergenic
ACCGTTACAGTTGCATCTGT+TGG 0.483048 1.3:-59303909 MS.gene44166:CDS
TGCAGAACTTGCTGCCAAGC+TGG 0.486136 1.3:-59303353 MS.gene44166:CDS
TCTTCCTACTTCACCACCAC+TGG 0.489968 1.3:+59303785 None:intergenic
AATAATATCTACAACAAGGC+AGG 0.494608 1.3:+59303188 None:intergenic
GCTTTCTGCAGTTGGTATTG+TGG 0.496447 1.3:-59303049 MS.gene44166:CDS
TGACAGTTTGTGCAGAGGAA+TGG 0.499633 1.3:+59302368 None:intergenic
CCTTCCCATGGCTTAGATAC+CGG 0.499863 1.3:+59302392 None:intergenic
TGCCTAGCAGATCAACCATC+TGG 0.501426 1.3:-59302452 MS.gene44166:CDS
TTTGTGAAGTATGGACAGGC+TGG 0.503204 1.3:-59304646 MS.gene44166:CDS
AAAATGTAGAAGAGAGGGAG+CGG 0.505425 1.3:-59303815 MS.gene44166:CDS
AATGATGTTGAAGAATCGCC+AGG 0.506246 1.3:+59304568 None:intergenic
CGTTTGTGATTATTGATGGA+TGG 0.510062 1.3:-59303932 MS.gene44166:CDS
AGAGAGAAACAACACCTCCC+TGG 0.510635 1.3:+59303862 None:intergenic
TTCTGCAGTTGGTATTGTGG+AGG 0.515246 1.3:-59303046 MS.gene44166:CDS
AGCTTGGCAGCAAGTTCTGC+AGG 0.516289 1.3:+59303355 None:intergenic
AGGTAGGGCCTCTTCGCTGT+TGG 0.519693 1.3:-59303662 MS.gene44166:CDS
ACAGCCAGTGGTGGTGAAGT+AGG 0.520024 1.3:-59303789 MS.gene44166:CDS
CCTGCTTACACCTTGCGGAA+TGG 0.526627 1.3:+59304138 None:intergenic
CGTTACAGTTGCATCTGTTG+GGG 0.531574 1.3:-59303907 MS.gene44166:CDS
TTGATTGGCAGATCCTTGAC+GGG 0.533037 1.3:-59304222 MS.gene44166:intron
GAAGCAACCACTTGTTCCGT+TGG 0.533409 1.3:-59304716 MS.gene44166:CDS
TGAGTAAGACTGAATTATCA+AGG 0.534598 1.3:-59304741 MS.gene44166:CDS
TCCTCCCTGCTTACACCTTG+CGG 0.535261 1.3:+59304133 None:intergenic
ATAAAATGATCGGTGTTCAC+TGG 0.535304 1.3:+59302419 None:intergenic
CCTCTTCGCTGTTGGCCTGG+TGG 0.536837 1.3:-59303654 MS.gene44166:CDS
AAATGTAGAAGAGAGGGAGC+GGG 0.539423 1.3:-59303814 MS.gene44166:CDS
TGTGGAGGAGTTATTTGAAG+AGG 0.540145 1.3:-59303031 MS.gene44166:CDS
CCTGGGTATCTAATATGCAT+CGG 0.544206 1.3:+59303880 None:intergenic
ATACCAGAATTTGTATTGGA+AGG 0.544653 1.3:+59302491 None:intergenic
TCACTCCGGTATCTAAGCCA+TGG 0.550001 1.3:-59302397 MS.gene44166:CDS
AGACTAATTATAGCTTCTGA+TGG 0.551958 1.3:-59303430 MS.gene44166:CDS
TCTAAGGTCAAGAGGGCTGA+AGG 0.552829 1.3:-59303220 MS.gene44166:CDS
TGTCACCAATCGATCTAGAA+AGG 0.554127 1.3:+59303625 None:intergenic
GAGAGAAACAACACCTCCCT+GGG 0.554466 1.3:+59303863 None:intergenic
AGGGAGCGGGTTACAGCCAG+TGG 0.559334 1.3:-59303801 MS.gene44166:CDS
GGAAGAAAATGTAGAAGAGA+GGG 0.560448 1.3:-59303820 MS.gene44166:CDS
CACTCCGGTATCTAAGCCAT+GGG 0.564768 1.3:-59302396 MS.gene44166:CDS
AAGGTGTAAGCAGGGAGGAA+TGG 0.566433 1.3:-59304129 MS.gene44166:CDS
CTAGAAAGGCACAATCCACC+AGG 0.566834 1.3:+59303639 None:intergenic
CCGATGCATATTAGATACCC+AGG 0.567386 1.3:-59303880 MS.gene44166:CDS
TCTTCTGACAGTTTGTGCAG+AGG 0.570739 1.3:+59302363 None:intergenic
TTGTGCCTTTCTAGATCGAT+TGG 0.574242 1.3:-59303630 MS.gene44166:CDS
CACCAGATGGTTGATCTGCT+AGG 0.577655 1.3:+59302450 None:intergenic
AGGTACACCACGACATGACT+TGG 0.578313 1.3:+59303375 None:intergenic
CAGGAAGCTCTAAGGTCAAG+AGG 0.580501 1.3:-59303228 MS.gene44166:CDS
ACCTTCACTTGCTTAACATG+TGG 0.582513 1.3:+59303573 None:intergenic
CAGAGGAATGGACCTTCCCA+TGG 0.585428 1.3:+59302380 None:intergenic
TCTCAAAACTCGACAAACAA+AGG 0.587947 1.3:-59303081 MS.gene44166:CDS
CTTGACAGTTGATCACAGAC+TGG 0.593422 1.3:-59303841 MS.gene44166:CDS
AAGAAGAAAGACGCCATGGA+AGG 0.594109 1.3:-59302344 MS.gene44166:CDS
ATCGATTGGTGACACCGATG+TGG 0.601496 1.3:-59303616 MS.gene44166:CDS
GCAGATCAACCATCTGGTGA+TGG 0.602774 1.3:-59302446 MS.gene44166:CDS
TACCGGAGTGATAAAATGAT+CGG 0.613081 1.3:+59302409 None:intergenic
CATCACCTTAACCACCAGCT+TGG 0.613564 1.3:+59303339 None:intergenic
CCACCAGGCCAACAGCGAAG+AGG 0.616089 1.3:+59303654 None:intergenic
GTCATGAGTGCCATTCCGCA+AGG 0.616945 1.3:-59304148 MS.gene44166:CDS
TGCAACACAGGATAGTCTGA+AGG 0.617016 1.3:+59303165 None:intergenic
TCGATTGGTGACACCGATGT+GGG 0.617709 1.3:-59303615 MS.gene44166:CDS
AAGGTTGCCAACGGAACAAG+TGG 0.618923 1.3:+59304709 None:intergenic
ATATACCATTATGCCCGTCA+AGG 0.625718 1.3:+59304209 None:intergenic
GAGCGGGTTACAGCCAGTGG+TGG 0.626341 1.3:-59303798 MS.gene44166:CDS
AGGAAGCTCTAAGGTCAAGA+GGG 0.627250 1.3:-59303227 MS.gene44166:CDS
ACCACATGTTAAGCAAGTGA+AGG 0.627901 1.3:-59303574 MS.gene44166:intron
TCAGAAGAAGAAAGACGCCA+TGG 0.630309 1.3:-59302348 MS.gene44166:CDS
CCCAACAGATGCAACTGTAA+CGG 0.630410 1.3:+59303908 None:intergenic
CGGCCGATCAAGGTTGCCAA+CGG 0.631386 1.3:+59304700 None:intergenic
TCCTAGCTTTCAAATGCTGG+CGG 0.631854 1.3:-59303454 MS.gene44166:intron
CCGGTATCTAAGCCATGGGA+AGG 0.632107 1.3:-59302392 MS.gene44166:CDS
AAAGCGACAACTAGAGCCCG+AGG 0.640273 1.3:+59304094 None:intergenic
CAACGTATTCGGAGGCAATG+AGG 0.642352 1.3:-59303760 MS.gene44166:intron
CGATGCATATTAGATACCCA+GGG 0.643134 1.3:-59303879 MS.gene44166:CDS
ACTGGTAAACCATCACCAGA+TGG 0.656343 1.3:+59302437 None:intergenic
CATTCCGCAAGGTGTAAGCA+GGG 0.660301 1.3:-59304137 MS.gene44166:CDS
GGAAGACTCAACGTATTCGG+AGG 0.678380 1.3:-59303768 MS.gene44166:CDS
AACGATATACTCTCCCACAT+CGG 0.681964 1.3:+59303602 None:intergenic
AGAACTTGCTGCCAAGCTGG+TGG 0.683075 1.3:-59303350 MS.gene44166:CDS
ACGGCTGCCAAGTCATGTCG+TGG 0.691102 1.3:-59303382 MS.gene44166:CDS
AAGGAATAATATCTACAACA+AGG 0.708500 1.3:+59303184 None:intergenic
TCCGCAAGGTGTAAGCAGGG+AGG 0.718559 1.3:-59304134 MS.gene44166:CDS
GTTCTGCAATGCTTACAGAG+AGG 0.751165 1.3:-59303008 MS.gene44166:intron
TGCATATTAGATACCCAGGG+AGG 0.753250 1.3:-59303876 MS.gene44166:CDS
CTGCTAGGCAAACTGCACAG+CGG 0.759343 1.3:+59302465 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTACTTTTTACAT+CGG + chr1.3:59304456-59304475 None:intergenic 15.0%
!!! TTGAAAATGCATTTTATAAA+TGG - chr1.3:59304271-59304290 MS.gene44166:intron 15.0%
!! AAGAACTACTAAACTTTAAA+AGG + chr1.3:59304133-59304152 None:intergenic 20.0%
!! ATAAGATCTTATGAATGAAT+TGG - chr1.3:59302665-59302684 MS.gene44166:intron 20.0%
!! TAAGGAAGAAATTATATGAA+AGG + chr1.3:59303361-59303380 None:intergenic 20.0%
!!! AAAATTTAATTTTACCTGCA+GGG - chr1.3:59303096-59303115 MS.gene44166:CDS 20.0%
!!! CATTTGATTATGAATCTTTT+TGG + chr1.3:59304398-59304417 None:intergenic 20.0%
!!! TAAAATTTAATTTTACCTGC+AGG - chr1.3:59303095-59303114 MS.gene44166:CDS 20.0%
!!! TTTTTGTTTTCTTAAAATGC+AGG - chr1.3:59304544-59304563 MS.gene44166:intron 20.0%
! AAGGAATAATATCTACAACA+AGG + chr1.3:59303892-59303911 None:intergenic 25.0%
! ATCAATAAGGAATTCTTGAT+TGG - chr1.3:59302836-59302855 MS.gene44166:intron 25.0%
! ATTGATATCATAACCATTGT+GGG + chr1.3:59302822-59302841 None:intergenic 25.0%
! CAATGGTTATGATATCAATA+AGG - chr1.3:59302823-59302842 MS.gene44166:intron 25.0%
! GATTGGAACAAAAATGTTTA+GGG - chr1.3:59304173-59304192 MS.gene44166:CDS 25.0%
! TATTGATATCATAACCATTG+TGG + chr1.3:59302823-59302842 None:intergenic 25.0%
! TGATTGGAACAAAAATGTTT+AGG - chr1.3:59304172-59304191 MS.gene44166:CDS 25.0%
! TTCCTTAATATGTTTAGGTA+GGG - chr1.3:59303396-59303415 MS.gene44166:CDS 25.0%
!! AGTTTTCTTTGTATCCTTAT+TGG - chr1.3:59303044-59303063 MS.gene44166:CDS 25.0%
!! TTTGGTTCCTTAATATGTTT+AGG - chr1.3:59303391-59303410 MS.gene44166:CDS 25.0%
!!! AAATTTAATTTTACCTGCAG+GGG - chr1.3:59303097-59303116 MS.gene44166:CDS 25.0%
AAAGGGAATGTATCTTTCTT+AGG - chr1.3:59302712-59302731 MS.gene44166:intron 30.0%
AATAATATCTACAACAAGGC+AGG + chr1.3:59303888-59303907 None:intergenic 30.0%
AGACTAATTATAGCTTCTGA+TGG - chr1.3:59303643-59303662 MS.gene44166:CDS 30.0%
AGCACATTATGTTTCTTTCT+TGG + chr1.3:59303947-59303966 None:intergenic 30.0%
AGTAAACACCTTCACATAAA+AGG + chr1.3:59303562-59303581 None:intergenic 30.0%
ATACCAGAATTTGTATTGGA+AGG + chr1.3:59304585-59304604 None:intergenic 30.0%
GAACCAAAACAAGTTAGATA+AGG + chr1.3:59303379-59303398 None:intergenic 30.0%
GAATTCAAGTCTCATTATGT+TGG - chr1.3:59303788-59303807 MS.gene44166:CDS 30.0%
GACATGGAATTTCAGAAAAA+AGG - chr1.3:59303012-59303031 MS.gene44166:CDS 30.0%
GTAAACACCTTCACATAAAA+GGG + chr1.3:59303561-59303580 None:intergenic 30.0%
GTGAATACCAGAATTTGTAT+TGG + chr1.3:59304589-59304608 None:intergenic 30.0%
GTTCCTTAATATGTTTAGGT+AGG - chr1.3:59303395-59303414 MS.gene44166:CDS 30.0%
TAAGAAAGATACATTCCCTT+TGG + chr1.3:59302713-59302732 None:intergenic 30.0%
TATAGCTTCTGATGGAATTT+GGG - chr1.3:59303651-59303670 MS.gene44166:CDS 30.0%
TGAGTAAGACTGAATTATCA+AGG - chr1.3:59302332-59302351 MS.gene44166:CDS 30.0%
TTAGTGAAATGTTTGATGAG+AGG - chr1.3:59302607-59302626 MS.gene44166:intron 30.0%
TTATAGCTTCTGATGGAATT+TGG - chr1.3:59303650-59303669 MS.gene44166:CDS 30.0%
! AACATCATTTTCTGTTTTCG+CGG - chr1.3:59302519-59302538 MS.gene44166:intron 30.0%
!! AACTAGAGAAAGTGCATTTT+CGG + chr1.3:59304350-59304369 None:intergenic 30.0%
!! TTTGATGAGAGGTATTTGAT+TGG - chr1.3:59302618-59302637 MS.gene44166:intron 30.0%
!!! ATGTCCTTATTTTCCCATTT+TGG - chr1.3:59304497-59304516 MS.gene44166:intron 30.0%
!!! CTTCCTTATCTAACTTGTTT+TGG - chr1.3:59303373-59303392 MS.gene44166:CDS 30.0%
!!! TCTATTGTTTTAACCTGAAC+AGG - chr1.3:59303826-59303845 MS.gene44166:CDS 30.0%
!!! TGTCCTTATTTTCCCATTTT+GGG - chr1.3:59304498-59304517 MS.gene44166:intron 30.0%
AAAGCTAGGAAACAAATCAG+GGG + chr1.3:59303609-59303628 None:intergenic 35.0%
ACACATGAATACACCCAAAA+TGG + chr1.3:59304514-59304533 None:intergenic 35.0%
ACACCCAAAATGGGAAAATA+AGG + chr1.3:59304504-59304523 None:intergenic 35.0%
ACTAATAAGCTTTCTGCAGT+TGG - chr1.3:59304016-59304035 MS.gene44166:intron 35.0%
ACTTGCTTAACATGTGGTAT+TGG + chr1.3:59303497-59303516 None:intergenic 35.0%
CACATGAATACACCCAAAAT+GGG + chr1.3:59304513-59304532 None:intergenic 35.0%
CGTTTGTGATTATTGATGGA+TGG - chr1.3:59303141-59303160 MS.gene44166:CDS 35.0%
GAAAGCTAGGAAACAAATCA+GGG + chr1.3:59303610-59303629 None:intergenic 35.0%
GCTACGTTTGTGATTATTGA+TGG - chr1.3:59303137-59303156 MS.gene44166:CDS 35.0%
GGAAGAAAATGTAGAAGAGA+GGG - chr1.3:59303253-59303272 MS.gene44166:intron 35.0%
GGATACTTTGTCTTCTGATA+CGG - chr1.3:59303672-59303691 MS.gene44166:intron 35.0%
GTTCCTTCCAATACAAATTC+TGG - chr1.3:59304579-59304598 MS.gene44166:CDS 35.0%
TACCGGAGTGATAAAATGAT+CGG + chr1.3:59304667-59304686 None:intergenic 35.0%
TCTCAAAACTCGACAAACAA+AGG - chr1.3:59303992-59304011 MS.gene44166:intron 35.0%
TGAAAGCTAGGAAACAAATC+AGG + chr1.3:59303611-59303630 None:intergenic 35.0%
TGAGCTACGAAATGGAAAAA+CGG - chr1.3:59302396-59302415 MS.gene44166:CDS 35.0%
TGGAAGAAAATGTAGAAGAG+AGG - chr1.3:59303252-59303271 MS.gene44166:intron 35.0%
! ACATTTTCTCAGTGATCAAG+TGG - chr1.3:59302782-59302801 MS.gene44166:intron 35.0%
! ATAAAATGATCGGTGTTCAC+TGG + chr1.3:59304657-59304676 None:intergenic 35.0%
! GTTAAGGTGATGGTTATTTG+TGG - chr1.3:59303745-59303764 MS.gene44166:intron 35.0%
! GTTTTCTTAAAATGCAGGCT+AGG - chr1.3:59304549-59304568 MS.gene44166:intron 35.0%
! TGTTTAGGGTCTGTTTACTT+CGG - chr1.3:59304187-59304206 MS.gene44166:CDS 35.0%
! TTCCAATCACTTGTTGATGA+TGG + chr1.3:59304161-59304180 None:intergenic 35.0%
! TTTTTCCATTTCGTAGCTCA+CGG + chr1.3:59302396-59302415 None:intergenic 35.0%
!! TTCTAAAGTTCAATGCTCAC+TGG - chr1.3:59303581-59303600 MS.gene44166:CDS 35.0%
!!! GGCAGTGTTCTTATTTTTTG+AGG - chr1.3:59303766-59303785 MS.gene44166:CDS 35.0%
!!! GTGAGTTGGTTTCATTTTCA+TGG - chr1.3:59303521-59303540 MS.gene44166:intron 35.0%
AAAATGTAGAAGAGAGGGAG+CGG - chr1.3:59303258-59303277 MS.gene44166:intron 40.0%
AACGATATACTCTCCCACAT+CGG + chr1.3:59303474-59303493 None:intergenic 40.0%
AATGATGTTGAAGAATCGCC+AGG + chr1.3:59302508-59302527 None:intergenic 40.0%
ACCACATGTTAAGCAAGTGA+AGG - chr1.3:59303499-59303518 MS.gene44166:intron 40.0%
ACCTTCACTTGCTTAACATG+TGG + chr1.3:59303503-59303522 None:intergenic 40.0%
ATATACCATTATGCCCGTCA+AGG + chr1.3:59302867-59302886 None:intergenic 40.0%
CCTGGGTATCTAATATGCAT+CGG + chr1.3:59303196-59303215 None:intergenic 40.0%
CGATGCATATTAGATACCCA+GGG - chr1.3:59303194-59303213 MS.gene44166:CDS 40.0%
CTGGGTATCTAATATGCATC+GGG + chr1.3:59303195-59303214 None:intergenic 40.0%
CTGTCATTGAGAGAACATGA+AGG + chr1.3:59304375-59304394 None:intergenic 40.0%
CTTAAAATGCAGGCTAGGTA+AGG - chr1.3:59304554-59304573 MS.gene44166:intron 40.0%
GAACAAGTACAGCTCCAATA+AGG + chr1.3:59303061-59303080 None:intergenic 40.0%
GCGTCAATTTATTCCCACAA+TGG - chr1.3:59302806-59302825 MS.gene44166:intron 40.0%
GGCCCTACCTAAACATATTA+AGG + chr1.3:59303401-59303420 None:intergenic 40.0%
GTAGGAAGACTCAACGTATT+CGG - chr1.3:59303302-59303321 MS.gene44166:intron 40.0%
GTGCTTAGTTCACTTCTCTT+TGG - chr1.3:59303962-59303981 MS.gene44166:intron 40.0%
GTGGAGGAGTTATTTGAAGA+GGG - chr1.3:59304043-59304062 MS.gene44166:intron 40.0%
TGCCATCATCAACAAGTGAT+TGG - chr1.3:59304156-59304175 MS.gene44166:CDS 40.0%
TGTCACCAATCGATCTAGAA+AGG + chr1.3:59303451-59303470 None:intergenic 40.0%
TGTGGAGGAGTTATTTGAAG+AGG - chr1.3:59304042-59304061 MS.gene44166:intron 40.0%
TGTTTCTTTCTTGGAGTTGC+TGG + chr1.3:59303938-59303957 None:intergenic 40.0%
TTAAGCAAGTGAAGGTGAGT+TGG - chr1.3:59303507-59303526 MS.gene44166:intron 40.0%
TTCCTTCGCAAAAATAGCAG+CGG + chr1.3:59302890-59302909 None:intergenic 40.0%
TTGTGCCTTTCTAGATCGAT+TGG - chr1.3:59303443-59303462 MS.gene44166:CDS 40.0%
! CACCGATCATTTTATCACTC+CGG - chr1.3:59304662-59304681 MS.gene44166:CDS 40.0%
! CGCTTTTGTGAAAACTGACA+TGG - chr1.3:59302996-59303015 MS.gene44166:intron 40.0%
! CTAGTGACCCTTTTATGTGA+AGG - chr1.3:59303551-59303570 MS.gene44166:intron 40.0%
! GAGAAGCCTTTTGTGAAGTA+TGG - chr1.3:59302418-59302437 MS.gene44166:CDS 40.0%
! GTTTAGGGTCTGTTTACTTC+GGG - chr1.3:59304188-59304207 MS.gene44166:CDS 40.0%
! GTTTCCTAGCTTTCAAATGC+TGG - chr1.3:59303616-59303635 MS.gene44166:CDS 40.0%
! TCATTTTCTGTTTTCGCGGT+AGG - chr1.3:59302523-59302542 MS.gene44166:intron 40.0%
! TTCTGCTTCTAGAGACCAAA+GGG - chr1.3:59302695-59302714 MS.gene44166:intron 40.0%
!!! TTACTTTTTACATCGGCAGC+TGG + chr1.3:59304449-59304468 None:intergenic 40.0%
AAATGTAGAAGAGAGGGAGC+GGG - chr1.3:59303259-59303278 MS.gene44166:intron 45.0%
AACCTGAACAGGAAGCTCTA+AGG - chr1.3:59303837-59303856 MS.gene44166:CDS 45.0%
AAGAAGAAAGACGCCATGGA+AGG - chr1.3:59304729-59304748 MS.gene44166:CDS 45.0%
AAGAATCGCCAGGAACTCTA+TGG + chr1.3:59302498-59302517 None:intergenic 45.0%
AAGACGCCATGGAAGGAAAA+AGG - chr1.3:59304736-59304755 MS.gene44166:CDS 45.0%
ACAGATTGCCATAGAGTTCC+TGG - chr1.3:59302487-59302506 MS.gene44166:CDS 45.0%
ACCGTTACAGTTGCATCTGT+TGG - chr1.3:59303164-59303183 MS.gene44166:CDS 45.0%
ACTGGTAAACCATCACCAGA+TGG + chr1.3:59304639-59304658 None:intergenic 45.0%
CCCAACAGATGCAACTGTAA+CGG + chr1.3:59303168-59303187 None:intergenic 45.0%
CCGATGCATATTAGATACCC+AGG - chr1.3:59303193-59303212 MS.gene44166:CDS 45.0%
CCGTTACAGTTGCATCTGTT+GGG - chr1.3:59303165-59303184 MS.gene44166:CDS 45.0%
CGTTACAGTTGCATCTGTTG+GGG - chr1.3:59303166-59303185 MS.gene44166:CDS 45.0%
CTTGACAGTTGATCACAGAC+TGG - chr1.3:59303232-59303251 MS.gene44166:intron 45.0%
CTTGATTGGCAGATCCTTGA+CGG - chr1.3:59302850-59302869 MS.gene44166:intron 45.0%
GCCTGTCCATACTTCACAAA+AGG + chr1.3:59302427-59302446 None:intergenic 45.0%
GTTCTGCAATGCTTACAGAG+AGG - chr1.3:59304065-59304084 MS.gene44166:CDS 45.0%
TCAGAAGAAGAAAGACGCCA+TGG - chr1.3:59304725-59304744 MS.gene44166:CDS 45.0%
TCTTCTGACAGTTTGTGCAG+AGG + chr1.3:59304713-59304732 None:intergenic 45.0%
TGACAGTTTGTGCAGAGGAA+TGG + chr1.3:59304708-59304727 None:intergenic 45.0%
TGCAACACAGGATAGTCTGA+AGG + chr1.3:59303911-59303930 None:intergenic 45.0%
TGCATATTAGATACCCAGGG+AGG - chr1.3:59303197-59303216 MS.gene44166:CDS 45.0%
TTGATTGGCAGATCCTTGAC+GGG - chr1.3:59302851-59302870 MS.gene44166:intron 45.0%
TTTGTGAAGTATGGACAGGC+TGG - chr1.3:59302427-59302446 MS.gene44166:CDS 45.0%
! GCCTTTTGTGAAGTATGGAC+AGG - chr1.3:59302423-59302442 MS.gene44166:CDS 45.0%
! GCTTTCTGCAGTTGGTATTG+TGG - chr1.3:59304024-59304043 MS.gene44166:intron 45.0%
! GTTCTGCTTCTAGAGACCAA+AGG - chr1.3:59302694-59302713 MS.gene44166:intron 45.0%
! TCAGGACCTTTTTCCTTCCA+TGG + chr1.3:59304745-59304764 None:intergenic 45.0%
! TCCTAGCTTTCAAATGCTGG+CGG - chr1.3:59303619-59303638 MS.gene44166:CDS 45.0%
! TTCTGCAGTTGGTATTGTGG+AGG - chr1.3:59304027-59304046 MS.gene44166:intron 45.0%
!! AGGAAGCTCTAAGGTCAAGA+GGG - chr1.3:59303846-59303865 MS.gene44166:CDS 45.0%
!! CAGGCTGGTTTGGCAAAAAA+AGG - chr1.3:59302442-59302461 MS.gene44166:CDS 45.0%
!!! ATCCGCTGCTATTTTTGCGA+AGG - chr1.3:59302885-59302904 MS.gene44166:intron 45.0%
AAGGTGTAAGCAGGGAGGAA+TGG - chr1.3:59302944-59302963 MS.gene44166:intron 50.0%
AAGGTTGCCAACGGAACAAG+TGG + chr1.3:59302367-59302386 None:intergenic 50.0%
AGAGAGAAACAACACCTCCC+TGG + chr1.3:59303214-59303233 None:intergenic 50.0%
AGGTACACCACGACATGACT+TGG + chr1.3:59303701-59303720 None:intergenic 50.0%
CAACGTATTCGGAGGCAATG+AGG - chr1.3:59303313-59303332 MS.gene44166:intron 50.0%
CAAGCTGGTGGTTAAGGTGA+TGG - chr1.3:59303735-59303754 MS.gene44166:intron 50.0%
CACCAGATGGTTGATCTGCT+AGG + chr1.3:59304626-59304645 None:intergenic 50.0%
CACTCCGGTATCTAAGCCAT+GGG - chr1.3:59304677-59304696 MS.gene44166:CDS 50.0%
CATCACCTTAACCACCAGCT+TGG + chr1.3:59303737-59303756 None:intergenic 50.0%
CATTCCGCAAGGTGTAAGCA+GGG - chr1.3:59302936-59302955 MS.gene44166:intron 50.0%
CCTTCCCATGGCTTAGATAC+CGG + chr1.3:59304684-59304703 None:intergenic 50.0%
GAAGCAACCACTTGTTCCGT+TGG - chr1.3:59302357-59302376 MS.gene44166:CDS 50.0%
GACCTTAGAGCTTCCTGTTC+AGG + chr1.3:59303842-59303861 None:intergenic 50.0%
GAGAGAAACAACACCTCCCT+GGG + chr1.3:59303213-59303232 None:intergenic 50.0%
GCAGATCAACCATCTGGTGA+TGG - chr1.3:59304627-59304646 MS.gene44166:CDS 50.0%
GGAAGACTCAACGTATTCGG+AGG - chr1.3:59303305-59303324 MS.gene44166:intron 50.0%
GTTCCGTTGGCAACCTTGAT+CGG - chr1.3:59302370-59302389 MS.gene44166:CDS 50.0%
TCACTCCGGTATCTAAGCCA+TGG - chr1.3:59304676-59304695 MS.gene44166:CDS 50.0%
TCCGCCAGCATTTGAAAGCT+AGG + chr1.3:59303623-59303642 None:intergenic 50.0%
TCTTCCTACTTCACCACCAC+TGG + chr1.3:59303291-59303310 None:intergenic 50.0%
TGCCTAGCAGATCAACCATC+TGG - chr1.3:59304621-59304640 MS.gene44166:CDS 50.0%
TTACCTGCAGGGGAAACTTC+TGG - chr1.3:59303107-59303126 MS.gene44166:CDS 50.0%
! AATGGCTTCAAGCACTGCCT+CGG - chr1.3:59302962-59302981 MS.gene44166:intron 50.0%
! ATCGATTGGTGACACCGATG+TGG - chr1.3:59303457-59303476 MS.gene44166:intron 50.0%
! CAGATCCTTGACGGGCATAA+TGG - chr1.3:59302859-59302878 MS.gene44166:intron 50.0%
! CTAGAAAGGCACAATCCACC+AGG + chr1.3:59303437-59303456 None:intergenic 50.0%
! GAAGTATGGACAGGCTGGTT+TGG - chr1.3:59302432-59302451 MS.gene44166:CDS 50.0%
! TCGATTGGTGACACCGATGT+GGG - chr1.3:59303458-59303477 MS.gene44166:intron 50.0%
! TCTAAGGTCAAGAGGGCTGA+AGG - chr1.3:59303853-59303872 MS.gene44166:CDS 50.0%
!! CAGGAAGCTCTAAGGTCAAG+AGG - chr1.3:59303845-59303864 MS.gene44166:CDS 50.0%
AAAGCGACAACTAGAGCCCG+AGG + chr1.3:59302982-59303001 None:intergenic 55.0%
AGCTTGGCAGCAAGTTCTGC+AGG + chr1.3:59303721-59303740 None:intergenic 55.0%
ATCGGCCGTGAGCTACGAAA+TGG - chr1.3:59302388-59302407 MS.gene44166:CDS 55.0%
CAGAGGAATGGACCTTCCCA+TGG + chr1.3:59304696-59304715 None:intergenic 55.0%
CCATTCCGCAAGGTGTAAGC+AGG - chr1.3:59302935-59302954 MS.gene44166:intron 55.0%
CCGGTATCTAAGCCATGGGA+AGG - chr1.3:59304681-59304700 MS.gene44166:CDS 55.0%
CCTGCTTACACCTTGCGGAA+TGG + chr1.3:59302938-59302957 None:intergenic 55.0%
CTGCTAGGCAAACTGCACAG+CGG + chr1.3:59304611-59304630 None:intergenic 55.0%
GTTCCAGAAGTTTCCCCTGC+AGG + chr1.3:59303113-59303132 None:intergenic 55.0%
GTTGCTGGCATCTGCAACAC+AGG + chr1.3:59303923-59303942 None:intergenic 55.0%
TCCTCCCTGCTTACACCTTG+CGG + chr1.3:59302943-59302962 None:intergenic 55.0%
TGCTGCCAAGCTGGTGGTTA+AGG - chr1.3:59303729-59303748 MS.gene44166:intron 55.0%
! ACAGCCAGTGGTGGTGAAGT+AGG - chr1.3:59303284-59303303 MS.gene44166:intron 55.0%
! ATGGCTTCAAGCACTGCCTC+GGG - chr1.3:59302963-59302982 MS.gene44166:intron 55.0%
! GTCATGAGTGCCATTCCGCA+AGG - chr1.3:59302925-59302944 MS.gene44166:intron 55.0%
!! AGAACTTGCTGCCAAGCTGG+TGG - chr1.3:59303723-59303742 MS.gene44166:intron 55.0%
!! TGCAGAACTTGCTGCCAAGC+TGG - chr1.3:59303720-59303739 MS.gene44166:intron 55.0%
ACGGCTGCCAAGTCATGTCG+TGG - chr1.3:59303691-59303710 MS.gene44166:intron 60.0%
AGGTAGGGCCTCTTCGCTGT+TGG - chr1.3:59303411-59303430 MS.gene44166:CDS 60.0%
CGGCCGATCAAGGTTGCCAA+CGG + chr1.3:59302376-59302395 None:intergenic 60.0%
TCCGCAAGGTGTAAGCAGGG+AGG - chr1.3:59302939-59302958 MS.gene44166:intron 60.0%
TCGTAGCTCACGGCCGATCA+AGG + chr1.3:59302386-59302405 None:intergenic 60.0%
CCACCAGGCCAACAGCGAAG+AGG + chr1.3:59303422-59303441 None:intergenic 65.0%
! CCTCTTCGCTGTTGGCCTGG+TGG - chr1.3:59303419-59303438 MS.gene44166:CDS 65.0%
!! AGGGAGCGGGTTACAGCCAG+TGG - chr1.3:59303272-59303291 MS.gene44166:intron 65.0%
!! GAGCGGGTTACAGCCAGTGG+TGG - chr1.3:59303275-59303294 MS.gene44166:intron 65.0%
GGGCCTCTTCGCTGTTGGCC+TGG - chr1.3:59303416-59303435 MS.gene44166:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 59302331 59304764 59302331 ID=MS.gene44166
chr1.3 mRNA 59302331 59304764 59302331 ID=MS.gene44166.t1;Parent=MS.gene44166
chr1.3 exon 59304555 59304764 59304555 ID=MS.gene44166.t1.exon1;Parent=MS.gene44166.t1
chr1.3 CDS 59304555 59304764 59304555 ID=cds.MS.gene44166.t1;Parent=MS.gene44166.t1
chr1.3 exon 59304062 59304233 59304062 ID=MS.gene44166.t1.exon2;Parent=MS.gene44166.t1
chr1.3 CDS 59304062 59304233 59304062 ID=cds.MS.gene44166.t1;Parent=MS.gene44166.t1
chr1.3 exon 59303761 59303978 59303761 ID=MS.gene44166.t1.exon3;Parent=MS.gene44166.t1
chr1.3 CDS 59303761 59303978 59303761 ID=cds.MS.gene44166.t1;Parent=MS.gene44166.t1
chr1.3 exon 59303575 59303682 59303575 ID=MS.gene44166.t1.exon4;Parent=MS.gene44166.t1
chr1.3 CDS 59303575 59303682 59303575 ID=cds.MS.gene44166.t1;Parent=MS.gene44166.t1
chr1.3 exon 59303345 59303470 59303345 ID=MS.gene44166.t1.exon5;Parent=MS.gene44166.t1
chr1.3 CDS 59303345 59303470 59303345 ID=cds.MS.gene44166.t1;Parent=MS.gene44166.t1
chr1.3 exon 59303009 59303247 59303009 ID=MS.gene44166.t1.exon6;Parent=MS.gene44166.t1
chr1.3 CDS 59303009 59303247 59303009 ID=cds.MS.gene44166.t1;Parent=MS.gene44166.t1
chr1.3 exon 59302331 59302529 59302331 ID=MS.gene44166.t1.exon7;Parent=MS.gene44166.t1
chr1.3 CDS 59302331 59302529 59302331 ID=cds.MS.gene44166.t1;Parent=MS.gene44166.t1
Gene Sequence

>MS.gene44166

ATGAGTAAGACTGAATTATCAAGGATGAAGCAACCACTTGTTCCGTTGGCAACCTTGATCGGCCGTGAGCTACGAAATGGAAAAACGGAGAAGCCTTTTGTGAAGTATGGACAGGCTGGTTTGGCAAAAAAAGGAGAAGATTACTTTCTAATCAAGACAGATTGCCATAGAGTTCCTGGCGATTCTTCAACATCATTTTCTGTTTTCGCGGTAGGTATCATACACGTTCTTTGTTTACATGTGTTTTTAATTAACTTGATTGTCATTCTTTTGTTTTTAGTGAAATGTTTGATGAGAGGTATTTGATTGGTAAGAAATGAAAATATTGCTTAAGATAAGATCTTATGAATGAATTGGCAATTTGTTCTGCTTCTAGAGACCAAAGGGAATGTATCTTTCTTAGGTTTAGTACATAAATGAGAGTTGAAGTGTGTGATTTCTATCTTATCTTACATTTTCTCAGTGATCAAGTGGAGCGTCAATTTATTCCCACAATGGTTATGATATCAATAAGGAATTCTTGATTGGCAGATCCTTGACGGGCATAATGGTATATCCGCTGCTATTTTTGCGAAGGAAAACATAATAAACAATGTCATGAGTGCCATTCCGCAAGGTGTAAGCAGGGAGGAATGGCTTCAAGCACTGCCTCGGGCTCTAGTTGTCGCTTTTGTGAAAACTGACATGGAATTTCAGAAAAAAGGTACACACACAGTTTTCTTTGTATCCTTATTGGAGCTGTACTTGTTCATGTACACTCATTCTAAAATTTAATTTTACCTGCAGGGGAAACTTCTGGAACGACAGCTACGTTTGTGATTATTGATGGATGGACCGTTACAGTTGCATCTGTTGGGGATTCCCGATGCATATTAGATACCCAGGGAGGTGTTGTTTCTCTCTTGACAGTTGATCACAGACTGGAAGAAAATGTAGAAGAGAGGGAGCGGGTTACAGCCAGTGGTGGTGAAGTAGGAAGACTCAACGTATTCGGAGGCAATGAGGTATGATGTGTAATCTTCATATTCCTTTCATATAATTTCTTCCTTATCTAACTTGTTTTGGTTCCTTAATATGTTTAGGTAGGGCCTCTTCGCTGTTGGCCTGGTGGATTGTGCCTTTCTAGATCGATTGGTGACACCGATGTGGGAGAGTATATCGTTCCAATACCACATGTTAAGCAAGTGAAGGTGAGTTGGTTTCATTTTCATGGTTTTAGTCTAGTGACCCTTTTATGTGAAGGTGTTTACTTCTAAAGTTCAATGCTCACTGGCTCCCCTGATTTGTTTCCTAGCTTTCAAATGCTGGCGGAAGACTAATTATAGCTTCTGATGGAATTTGGGATACTTTGTCTTCTGATACGGCTGCCAAGTCATGTCGTGGTGTACCTGCAGAACTTGCTGCCAAGCTGGTGGTTAAGGTGATGGTTATTTGTGGCAGTGTTCTTATTTTTTGAGGAATTCAAGTCTCATTATGTTGGTTACTCAATGCTTTGTCTATTGTTTTAACCTGAACAGGAAGCTCTAAGGTCAAGAGGGCTGAAGGATGATACAACCTGCCTTGTTGTAGATATTATTCCTTCAGACTATCCTGTGTTGCAGATGCCAGCAACTCCAAGAAAGAAACATAATGTGCTTAGTTCACTTCTCTTTGGAAAGAAATCTCAAAACTCGACAAACAAAGGAACTAATAAGCTTTCTGCAGTTGGTATTGTGGAGGAGTTATTTGAAGAGGGTTCTGCAATGCTTACAGAGAGGTCACTCTTAGACTATAACTGCAAAATTTATATTTGCATGCTGCCTTTTAAAGTTTAGTAGTTCTTTTGTGCCATCATCAACAAGTGATTGGAACAAAAATGTTTAGGGTCTGTTTACTTCGGGTGTTTTCTGTTTTTCATTTTAACTGATAAAATAAATATTAGAGAGACATTATAGACATTTTTGAAAATGCATTTTATAAATGGATTCAATATTTCTGAAAATTCTGAAAAATGTCAAAACATTGTTGTTAGTGTTTCCGAAAATGCACTTTCTCTAGTTTGCCTTCATGTTCTCTCAATGACAGCCAAAAAGATTCATAATCAAATGTCATAAAGATTCATAATCAAATGTCATTCCAGCTGCCGATGTAAAAAGTAAAAATTGAAAATGTTTTTAGAAAGTAAACATGTCCTTATTTTCCCATTTTGGGTGTATTCATGTGTTTGATAAGATTTTTTGTTTTCTTAAAATGCAGGCTAGGTAAGGATGTTCCTTCCAATACAAATTCTGGTATTCACCGCTGTGCAGTTTGCCTAGCAGATCAACCATCTGGTGATGGTTTACCAGTGAACACCGATCATTTTATCACTCCGGTATCTAAGCCATGGGAAGGTCCATTCCTCTGCACAAACTGTCAGAAGAAGAAAGACGCCATGGAAGGAAAAAGGTCCTGA
Protein sequence

>MS.gene44166.t1

MSKTELSRMKQPLVPLATLIGRELRNGKTEKPFVKYGQAGLAKKGEDYFLIKTDCHRVPGDSSTSFSVFAILDGHNGISAAIFAKENIINNVMSAIPQGVSREEWLQALPRALVVAFVKTDMEFQKKGETSGTTATFVIIDGWTVTVASVGDSRCILDTQGGVVSLLTVDHRLEENVEERERVTASGGEVGRLNVFGGNEVGPLRCWPGGLCLSRSIGDTDVGEYIVPIPHVKQVKLSNAGGRLIIASDGIWDTLSSDTAAKSCRGVPAELAAKLVVKEALRSRGLKDDTTCLVVDIIPSDYPVLQMPATPRKKHNVLSSLLFGKKSQNSTNKGTNKLSAVGIVEELFEEGSAMLTERLGKDVPSNTNSGIHRCAVCLADQPSGDGLPVNTDHFITPVSKPWEGPFLCTNCQKKKDAMEGKRS