AlfalfaGEDB | MS.gene44473

M. sativa cultivar XinJiangDaYe / MS.gene44473

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF00583.28

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44473.t1	XP_013457495.1	97.1	411	12	0	1	411	1	411	1.30E-236	828.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44473.t1	O64815	70.1	415	112	4	5	411	3	413	1.0e-168	594.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44473.t1	A0A072UQ97	97.1	411	12	0	1	411	1	411	9.5e-237	828.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene44473.t1	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene015242	MS.gene44473	PPI
MS.gene09583	MS.gene44473	PPI
MS.gene43555	MS.gene44473	PPI
MS.gene44473	MS.gene43555	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44473.t1	MTR_4g095700	97.080	411	12	0	1	411	1	411	0.0	826
MS.gene44473.t1	MTR_5g015810	71.499	407	106	5	5	411	7	403	0.0	610
MS.gene44473.t1	MTR_5g015810	71.345	342	88	5	70	411	1	332	0.0	513
MS.gene44473.t1	MTR_5g015810	72.664	289	75	3	122	410	1	285	2.81e-157	445
MS.gene44473.t1	MTR_4g007130	47.494	419	182	9	3	411	19	409	1.10e-124	367
MS.gene44473.t1	MTR_3g465010	48.995	398	172	7	17	410	17	387	2.39e-120	355
MS.gene44473.t1	MTR_4g122030	32.212	416	195	14	7	411	11	350	9.43e-54	182

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44473.t1	AT2G23060	70.120	415	112	4	5	411	3	413	0.0	594
MS.gene44473.t1	AT4G37580	70.936	406	109	3	9	411	4	403	0.0	573
MS.gene44473.t1	AT4G37580	71.387	346	93	2	66	411	7	346	1.21e-174	491
MS.gene44473.t1	AT5G67430	62.315	406	127	9	6	411	7	386	1.92e-172	487
MS.gene44473.t1	AT2G23060	73.214	280	74	1	132	411	1	279	1.58e-152	432
MS.gene44473.t1	AT5G67430	62.757	341	106	8	70	410	1	320	4.06e-144	413
MS.gene44473.t1	AT2G30090	41.951	410	191	7	9	410	15	385	7.59e-99	300

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Find 85 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGAATCAATGTCACG+AGG	0.691646	8.1:+25634978	None:intergenic
AAACTTAATTAAAACTCTCT+AGG	0.411336	8.1:+25634399	None:intergenic
AACAACTCGCGTGATAGACA+AGG	0.641023	8.1:-25634754	MS.gene44473:CDS
AACTCTTAAGCCTAAAACAT+AGG	0.527965	8.1:+25635298	None:intergenic
AACTTAATTAAAACTCTCTA+GGG	0.509353	8.1:+25634400	None:intergenic
AAGAGGGAAAGTTTACCACT+GGG	0.649201	8.1:+25635635	None:intergenic
AAGATGGTGAAAGCTTTGTG+TGG	0.659803	8.1:-25634633	MS.gene44473:CDS
AATAGGGTCACCGTGCAAGT+CGG	0.663384	8.1:+25635610	None:intergenic
AATCATAGAAGAATGGGAAT+AGG	0.430636	8.1:-25635269	MS.gene44473:CDS
AATGGACCCACCAAGTTCGT+GGG	0.562889	8.1:-25634859	MS.gene44473:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTCTTAAGCCTAAAACAT+AGG	+	chr8.1:25634840-25634859	None:intergenic	30.0%
AATCATAGAAGAATGGGAAT+AGG	-	chr8.1:25634866-25634885	MS.gene44473:CDS	30.0%
ATCTAATGTATGGAATTGGT+GGG	-	chr8.1:25635461-25635480	MS.gene44473:intron	30.0%
ATTCCCATTCTTCTATGATT+TGG	+	chr8.1:25634866-25634885	None:intergenic	30.0%
CCAAACGGTTTGAAAAAATT+CGG	+	chr8.1:25635436-25635455	None:intergenic	30.0%
TCTCCAAATCATAGAAGAAT+GGG	-	chr8.1:25634860-25634879	MS.gene44473:CDS	30.0%
TGGTAGAAAAAATGGAACAA+TGG	-	chr8.1:25634897-25634916	MS.gene44473:CDS	30.0%
TGTGGTGTATTAAGAGATTA+GGG	-	chr8.1:25635644-25635663	MS.gene44473:CDS	30.0%
TTCTCCAAATCATAGAAGAA+TGG	-	chr8.1:25634859-25634878	MS.gene44473:CDS	30.0%
TTGAAAAAATTCGGAATCGA+AGG	+	chr8.1:25635427-25635446	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	25634407	25635750	25634407	ID=MS.gene44473
chr8.1	mRNA	25634407	25635750	25634407	ID=MS.gene44473.t1;Parent=MS.gene44473
chr8.1	exon	25635574	25635750	25635574	ID=MS.gene44473.t1.exon1;Parent=MS.gene44473.t1
chr8.1	CDS	25635574	25635750	25635574	ID=cds.MS.gene44473.t1;Parent=MS.gene44473.t1
chr8.1	exon	25634407	25635465	25634407	ID=MS.gene44473.t1.exon2;Parent=MS.gene44473.t1
chr8.1	CDS	25634407	25635465	25634407	ID=cds.MS.gene44473.t1;Parent=MS.gene44473.t1

Gene Sequence

>MS.gene44473

ATGGGTGAAGTAGTAGTTGTGAATGTGAGAGAGTTTGATCCTAATAAAGATAGAGAAAGTGTTGAAGCAGTTGAAAAAATATGTGAAGTTGGACCCAGTGGTAAACTTTCCCTCTTCACCGACTTGCACGGTGACCCTATTTGCAGAGTCCGTAACTCACCAACTTTTCTCATGCTGGTAAGTTATATAATTAAATCCAAAACCATATACTATAATAATGTATCATATCATATCAACAAAATATTTGATTAGTCCTAATCCTAACTTTGTTATTTTAATTTTCAGGTAGCAGAGATAGGCAATGAGACAGTCGGAATGATACGAGGTTGTATCAAAACCGTTACATGCGGCAAAAAACTCACAAGACCAACAAAAAACACCATCACAGAAACACCAAATTCCAATCATGTCCCTGTTTTCACTAAACTAGCCTATGTTTTAGGCTTAAGAGTTTCTCCAAATCATAGAAGAATGGGAATAGGACTAAAACTGGTAGAAAAAATGGAACAATGGTTTCGTGAAAATGGTGCTGAGTATTCATACATGGCTACTGAAAACGACAACGTTGCATCAGTTAAACTTTTCACCGACAAATGTGGTTACTCAAAATTCCGTACACCGTCAATCCTTGTTAACCCCGTTTTCAAACACCGTCTCAAAACATCATCGTCCAAAGTAACAATCCTTAAACTCACACCAAACGACGCTGAATTGCTATACCGTTACAAATTCTCCACCACTGAGTTTTTTCCTCGTGACATTGATTCTGTTTTAAAAAACAAACTTACTCTTGGAACCTTCTTAGCCGTTCCGAGAGACGGAAAATATGGGGCCGGATCAAATAACTGGTCCGGCTCCGAAAGCTTTTTAATGGACCCACCAAGTTCGTGGGCTTTAGTAAGTGTTTGGAACTGCAAAGACGTGTTTACCCTCGAAGTAAAAGGCGCGTCCCGCGTGAGACGTGTGTTAGCAAAAACAACTCGCGTGATAGACAAGGCTTTACCTTGGTTAAAATTACCTTCGATTCCGAATTTTTTCAAACCGTTTGGATTTCATCTAATGTATGGAATTGGTGGGGAAGGACCAGAAGTGTTGAAGATGGTGAAAGCTTTGTGTGGTTTTGCTCATAATCTTGCTATGGAAAATGGGTGTAGTGCTGTGGCTACTGAAGTTTCTAGCTGTGAACCGTTACGTTTTGCTATTCCTCATTGGAAGGTTTTGTCTTGTGAAGAAGATTTGTGGTGTATTAAGAGATTAGGGGAAGATTATAGTGATGGTTCTGTTGGTGATTGGACTAAATCCAAACCTGGGTTCTCCATTTTTGTTGACCCTAGAGAGTTTTAA

Protein sequence

>MS.gene44473.t1

MGEVVVVNVREFDPNKDRESVEAVEKICEVGPSGKLSLFTDLHGDPICRVRNSPTFLMLVAEIGNETVGMIRGCIKTVTCGKKLTRPTKNTITETPNSNHVPVFTKLAYVLGLRVSPNHRRMGIGLKLVEKMEQWFRENGAEYSYMATENDNVASVKLFTDKCGYSKFRTPSILVNPVFKHRLKTSSSKVTILKLTPNDAELLYRYKFSTTEFFPRDIDSVLKNKLTLGTFLAVPRDGKYGAGSNNWSGSESFLMDPPSSWALVSVWNCKDVFTLEVKGASRVRRVLAKTTRVIDKALPWLKLPSIPNFFKPFGFHLMYGIGGEGPEVLKMVKALCGFAHNLAMENGCSAVATEVSSCEPLRFAIPHWKVLSCEEDLWCIKRLGEDYSDGSVGDWTKSKPGFSIFVDPREF