AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45230.t1 MTR_7g012070 94.667 375 20 0 1 375 1 375 0.0 736
MS.gene45230.t1 MTR_7g012090 92.000 375 30 0 1 375 1 375 0.0 714
MS.gene45230.t1 MTR_7g012040 76.676 373 81 3 3 375 2 368 0.0 585
MS.gene45230.t1 MTR_7g011900 71.467 375 102 2 1 375 1 370 0.0 560
MS.gene45230.t1 MTR_7g012020 71.314 373 102 2 3 375 2 369 0.0 556
MS.gene45230.t1 MTR_7g011990 67.021 376 114 3 1 375 1 367 0.0 516
MS.gene45230.t1 MTR_2g055940 63.380 355 124 2 21 375 15 363 1.81e-167 472
MS.gene45230.t1 MTR_1g096310 58.511 376 151 3 1 375 1 372 1.17e-159 453
MS.gene45230.t1 MTR_7g012020 72.535 284 72 3 3 285 2 280 1.23e-147 419
MS.gene45230.t1 MTR_3g021440 54.090 379 161 7 1 375 1 370 1.27e-136 394
MS.gene45230.t1 MTR_3g021430 55.618 356 150 4 21 375 9 357 4.86e-135 390
MS.gene45230.t1 MTR_7g012020 73.438 256 63 2 3 258 2 252 1.16e-134 385
MS.gene45230.t1 MTR_1g036460 50.402 373 178 6 5 375 3 370 6.44e-130 377
MS.gene45230.t1 MTR_1g036490 49.866 373 180 6 5 375 3 370 1.92e-126 368
MS.gene45230.t1 MTR_1g036510 52.925 359 162 6 19 375 31 384 1.05e-123 362
MS.gene45230.t1 MTR_3g092900 45.938 357 182 7 21 375 16 363 3.87e-103 309
MS.gene45230.t1 MTR_5g098170 43.056 360 194 7 19 375 12 363 1.30e-95 290
MS.gene45230.t1 MTR_7g118300 43.011 372 202 7 5 375 13 375 2.28e-91 279
MS.gene45230.t1 MTR_1g050220 45.509 334 129 6 26 358 6 287 6.04e-85 260
MS.gene45230.t1 MTR_0591s0010 41.551 361 194 9 21 375 28 377 6.86e-84 260
MS.gene45230.t1 MTR_1g036510 53.257 261 116 5 19 278 31 286 6.73e-83 254
MS.gene45230.t1 MTR_8g068690 40.608 362 201 9 19 375 24 376 2.61e-82 256
MS.gene45230.t1 MTR_7g118320 40.390 359 201 8 21 375 13 362 8.94e-81 251
MS.gene45230.t1 MTR_8g068870 41.159 345 190 8 35 375 28 363 7.31e-80 249
MS.gene45230.t1 MTR_8g068750 39.554 359 204 8 21 375 28 377 2.33e-78 246
MS.gene45230.t1 MTR_8g024160 39.385 358 167 7 19 375 20 328 5.66e-76 238
MS.gene45230.t1 MTR_1g036510 51.046 239 111 5 19 256 31 264 6.54e-70 220
MS.gene45230.t1 MTR_6g078850 38.420 367 212 8 14 374 24 382 2.85e-69 226
MS.gene45230.t1 MTR_8g068570 39.144 327 160 7 21 343 28 319 3.51e-66 213
MS.gene45230.t1 MTR_6g060290 56.021 191 61 5 1 189 1 170 4.86e-61 194
MS.gene45230.t1 MTR_2g072790 35.799 338 200 8 27 359 14 339 2.28e-60 199
MS.gene45230.t1 MTR_8g068730 34.807 362 190 10 19 375 25 345 5.15e-60 197
MS.gene45230.t1 MTR_3g096070 35.262 363 191 10 9 367 2 324 2.88e-57 190
MS.gene45230.t1 MTR_3g096050 34.225 374 222 10 9 375 2 358 8.55e-57 189
MS.gene45230.t1 MTR_8g068690 40.769 260 143 6 21 277 27 278 1.26e-55 184
MS.gene45230.t1 MTR_3g083620 34.049 326 195 8 39 360 38 347 1.80e-51 176
MS.gene45230.t1 MTR_0079s0070 35.866 329 186 12 41 361 10 321 3.82e-50 171
MS.gene45230.t1 MTR_4g038440 35.758 330 187 12 40 361 43 355 8.54e-50 171
MS.gene45230.t1 MTR_0017s0100 32.059 340 209 11 41 374 44 367 1.76e-48 168
MS.gene45230.t1 MTR_1g076940 29.846 325 215 5 40 360 32 347 4.45e-48 167
MS.gene45230.t1 MTR_8g068600 40.851 235 128 6 46 277 27 253 3.17e-47 162
MS.gene45230.t1 MTR_5g074600 29.882 338 208 11 34 360 26 345 5.36e-46 161
MS.gene45230.t1 MTR_4g033015 32.647 340 210 8 40 370 32 361 6.07e-46 161
MS.gene45230.t1 MTR_4g033200 32.353 340 211 8 40 370 32 361 5.05e-45 159
MS.gene45230.t1 MTR_5g074680 28.857 350 223 10 34 374 27 359 2.65e-44 157
MS.gene45230.t1 MTR_4g088340 29.825 342 219 8 38 374 7 332 2.84e-44 156
MS.gene45230.t1 MTR_4g088345 30.117 342 218 8 38 374 30 355 5.17e-44 156
MS.gene45230.t1 MTR_4g033150 31.176 340 215 8 40 370 32 361 2.41e-43 154
MS.gene45230.t1 MTR_8g068740 34.783 276 136 8 19 290 25 260 1.45e-42 150
MS.gene45230.t1 MTR_7g024520 28.696 345 209 10 38 373 29 345 5.39e-42 150
MS.gene45230.t1 MTR_5g074580 28.736 348 221 10 34 373 27 355 1.53e-41 149
MS.gene45230.t1 MTR_7g014570 29.268 328 202 9 41 360 33 338 3.46e-41 148
MS.gene45230.t1 MTR_4g079160 29.781 366 226 11 1 360 1 341 5.38e-41 148
MS.gene45230.t1 MTR_7g024500 27.729 339 220 8 38 373 29 345 7.79e-41 147
MS.gene45230.t1 MTR_7g014510 27.914 326 214 7 41 360 33 343 1.46e-39 144
MS.gene45230.t1 MTR_4g088200 29.552 335 217 8 34 361 26 348 1.50e-39 144
MS.gene45230.t1 MTR_5g074540 28.657 335 198 10 34 360 26 327 1.87e-39 143
MS.gene45230.t1 MTR_4g088190 30.655 336 215 7 34 361 26 351 1.77e-38 141
MS.gene45230.t1 MTR_3g096050 34.926 272 162 7 9 278 2 260 4.69e-38 137
MS.gene45230.t1 MTR_5g074610 28.750 240 140 4 138 374 114 325 2.56e-33 127
MS.gene45230.t1 MTR_8g068620 53.211 109 51 0 169 277 2 110 3.74e-33 120
MS.gene45230.t1 MTR_4g033170 30.038 263 166 6 40 299 32 279 3.65e-32 124
MS.gene45230.t1 MTR_4g033085 29.771 262 166 6 40 298 32 278 1.24e-30 120
MS.gene45230.t1 MTR_1g076930 29.200 250 154 7 38 280 7 240 1.60e-30 117
MS.gene45230.t1 MTR_4g038440 34.568 243 139 8 41 278 44 271 7.62e-28 110
MS.gene45230.t1 MTR_0794s0020 29.386 228 147 6 40 263 32 249 5.62e-25 102
MS.gene45230.t1 MTR_6g060280 70.909 55 11 1 229 278 1 55 7.48e-19 80.5
MS.gene45230.t1 MTR_8g068760 39.000 100 59 2 277 375 135 233 1.04e-15 76.3
MS.gene45230.t1 MTR_8g068580 41.304 92 52 2 285 375 43 133 9.12e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45230.t1 AT5G54160 47.527 364 176 9 16 375 9 361 1.31e-103 310
MS.gene45230.t1 AT1G77520 41.061 358 198 7 25 375 30 381 1.35e-91 280
MS.gene45230.t1 AT1G51990 38.936 357 208 7 21 374 12 361 6.44e-83 257
MS.gene45230.t1 AT1G51990 38.375 357 210 7 21 374 12 361 2.43e-82 256
MS.gene45230.t1 AT1G63140 39.744 390 210 11 5 375 18 401 1.49e-79 249
MS.gene45230.t1 AT1G63140 39.896 386 207 11 9 375 2 381 1.96e-79 249
MS.gene45230.t1 AT1G77530 41.899 358 195 8 25 375 30 381 2.86e-78 246
MS.gene45230.t1 AT1G21100 40.056 357 199 10 17 365 15 364 8.48e-76 239
MS.gene45230.t1 AT1G21130 40.056 357 199 10 17 365 15 364 5.34e-75 237
MS.gene45230.t1 AT1G21110 40.336 357 198 10 17 365 15 364 3.08e-74 235
MS.gene45230.t1 AT1G21120 39.776 357 200 10 17 365 15 364 1.80e-73 233
MS.gene45230.t1 AT1G21120 38.845 381 209 12 2 365 39 412 7.82e-73 233
MS.gene45230.t1 AT1G33030 39.017 346 197 7 28 368 10 346 9.30e-73 231
MS.gene45230.t1 AT1G76790 39.084 371 206 10 16 375 4 365 2.59e-72 230
MS.gene45230.t1 AT5G53810 38.352 352 191 11 38 375 39 378 2.12e-69 223
MS.gene45230.t1 AT3G53140 34.118 340 208 7 27 359 14 344 1.29e-61 202
MS.gene45230.t1 AT1G62900 45.098 204 109 3 174 375 3 205 2.37e-57 186
MS.gene45230.t1 AT5G37170 37.931 319 157 9 65 375 49 334 1.67e-56 188
MS.gene45230.t1 AT5G37170 45.455 198 104 4 181 375 38 234 7.31e-53 175
MS.gene45230.t1 AT1G63140 39.519 291 154 8 5 278 18 303 1.02e-49 169
MS.gene45230.t1 AT5G37170 45.455 187 98 4 192 375 1 186 4.22e-49 164
MS.gene45230.t1 AT1G33030 37.647 255 148 5 28 280 10 255 5.89e-47 161
MS.gene45230.t1 AT1G33030 36.981 265 154 6 28 288 10 265 7.43e-47 161
MS.gene45230.t1 AT1G21130 40.299 268 147 8 17 278 15 275 2.25e-45 158
MS.gene45230.t1 AT1G77530 42.194 237 127 5 25 256 30 261 1.73e-42 149
MS.gene45230.t1 AT1G63140 37.681 276 150 8 5 263 18 288 5.49e-39 140
MS.gene45230.t1 AT4G35160 30.000 340 204 11 39 360 44 367 4.49e-38 140
MS.gene45230.t1 AT4G35150 34.483 203 123 3 168 360 108 310 4.81e-37 137

Find 80 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACAAACAACTTGGAATTT+AGG 0.243482 7.3:-85660130 None:intergenic
TTTGTTGATTCAAGATCTTC+TGG 0.247585 7.3:-85660001 None:intergenic
TTGAATCACCCAGCTTTCTT+AGG 0.281972 7.3:+85658673 MS.gene45230:CDS
GTTCTCTTTCTCATTGGTAT+AGG 0.295426 7.3:-85658257 None:intergenic
AGCATACGGTCAAGCCTATT+TGG 0.295943 7.3:-85658499 None:intergenic
AAGTCGGGGTATTGAGTTAT+TGG 0.300209 7.3:-85658475 None:intergenic
CATATCCTTTGTATATTTCA+AGG 0.318611 7.3:-85659300 None:intergenic
AGCATTATAGCATCTCCTTC+TGG 0.323700 7.3:-85659599 None:intergenic
TGTAGCTTGTGTTCCATAAT+TGG 0.338384 7.3:+85659891 MS.gene45230:intron
TGAAAACGAGGATAATTATA+TGG 0.346391 7.3:+85658633 MS.gene45230:CDS
AACGTATTCGAGAAAGTTCA+TGG 0.380926 7.3:+85659181 MS.gene45230:CDS
TTCTCGAATACGTTTATCTC+TGG 0.389709 7.3:-85659172 None:intergenic
CACAAAGCTTTGCCTTCAAA+TGG 0.393394 7.3:+85659950 MS.gene45230:CDS
ATAATGACTTTACCATTTGA+AGG 0.398844 7.3:-85659962 None:intergenic
TGAAATATACAAAGGATATG+AGG 0.400826 7.3:+85659303 MS.gene45230:CDS
AATACCCCGACTTACCAAAT+AGG 0.405341 7.3:+85658485 MS.gene45230:CDS
TCAAGCCTATTTGGTAAGTC+GGG 0.405485 7.3:-85658490 None:intergenic
TTCTCAAAGTATTCATAGAT+AGG 0.406331 7.3:-85659208 None:intergenic
AACTGCAGGAAACACCATAT+TGG 0.407144 7.3:-85658347 None:intergenic
CATGATTATAGCTTCCAATA+TGG 0.408040 7.3:+85658333 MS.gene45230:CDS
TGTCGTGCTTTCTCCATCCT+TGG 0.409600 7.3:+85660148 MS.gene45230:CDS
TACATGGTTCTCTTTCTCAT+TGG 0.411854 7.3:-85658263 None:intergenic
TATCACTATCATCTATTTGT+GGG 0.416372 7.3:-85658300 None:intergenic
GAGAGTTTGAGTAAACAATC+TGG 0.433426 7.3:+85660103 MS.gene45230:CDS
GGGTTAGAGTTTCCACTACA+TGG 0.438536 7.3:-85658279 None:intergenic
CTGCGCTTGCTTGCTAGCCA+CGG 0.445823 7.3:+85658520 MS.gene45230:CDS
TAGCAAGCAAGCGCAGCATA+CGG 0.457537 7.3:-85658513 None:intergenic
ATCATTGGTCCGAGTGGAAA+CGG 0.460804 7.3:-85658551 None:intergenic
GTGATATAGGTCGAATTTGA+AGG 0.465211 7.3:+85659135 MS.gene45230:intron
TTTCTCCATCCTTGGTGTGA+TGG 0.466015 7.3:+85660156 MS.gene45230:CDS
CTTGTTTGATATCATTGCTA+AGG 0.474167 7.3:+85658393 MS.gene45230:CDS
TACCACACTCCTAAGAAAGC+TGG 0.484096 7.3:-85658682 None:intergenic
CACCATGCTACCATCATCAT+TGG 0.485235 7.3:-85658566 None:intergenic
GTATCACTATCATCTATTTG+TGG 0.493357 7.3:-85658301 None:intergenic
GTTGATGTAGGAGGTGGAAA+TGG 0.497071 7.3:+85659340 MS.gene45230:CDS
GTCAAGCCTATTTGGTAAGT+CGG 0.499804 7.3:-85658491 None:intergenic
GTCTCCTGTCCGTTTCCACT+CGG 0.503554 7.3:+85658542 MS.gene45230:CDS
ATATCACATGAAAACAATGT+TGG 0.508642 7.3:+85658418 MS.gene45230:CDS
AGAAAGCACGACAAACAACT+TGG 0.515962 7.3:-85660138 None:intergenic
GTTTATCACAGCTGGTGGTA+AGG 0.522095 7.3:+85660060 MS.gene45230:CDS
AAGCTATAATCATGGCTAAG+AGG 0.523693 7.3:-85658324 None:intergenic
AAATGGTAAAGTCATTATTG+TGG 0.527665 7.3:+85659967 MS.gene45230:CDS
AACATGATGTTTATCACAGC+TGG 0.539235 7.3:+85660052 MS.gene45230:CDS
AAGTTCCATCACACCAAGGA+TGG 0.539267 7.3:-85660161 None:intergenic
GGTGCATGTTCAATCACTTG+TGG 0.542683 7.3:-85659424 None:intergenic
GAACATGCACCACCCTCTCC+AGG 0.544120 7.3:+85659436 MS.gene45230:CDS
CATATTGGAAGCTATAATCA+TGG 0.546612 7.3:-85658332 None:intergenic
GTTTATGCAATCACACCTCC+TGG 0.548500 7.3:+85658592 MS.gene45230:CDS
GTGCATGTTCAATCACTTGT+GGG 0.549965 7.3:-85659423 None:intergenic
GAAATATACAAAGGATATGA+GGG 0.554252 7.3:+85659304 MS.gene45230:CDS
ACCACACTCCTAAGAAAGCT+GGG 0.554693 7.3:-85658681 None:intergenic
TTAGGGATCGAACATGTTGG+AGG 0.564879 7.3:+85659557 MS.gene45230:intron
TGATGGACAAAGTATTTACC+AGG 0.566769 7.3:-85658610 None:intergenic
TGGTCCGAGTGGAAACGGAC+AGG 0.566921 7.3:-85658546 None:intergenic
TATTTAGGGATCGAACATGT+TGG 0.571558 7.3:+85659554 MS.gene45230:intron
ATATCAACACTGGTTGATGT+AGG 0.573750 7.3:+85659328 MS.gene45230:CDS
ACACTGGTTGATGTAGGAGG+TGG 0.577123 7.3:+85659334 MS.gene45230:CDS
TCCACTCGGACCAATGATGA+TGG 0.585835 7.3:+85658556 MS.gene45230:CDS
ATCACTATCATCTATTTGTG+GGG 0.587605 7.3:-85658299 None:intergenic
AGCTATAATCATGGCTAAGA+GGG 0.594260 7.3:-85658323 None:intergenic
AGGAGATGCTATAATGCTCA+AGG 0.599170 7.3:+85659604 MS.gene45230:CDS
ACCCAGCTTTCTTAGGAGTG+TGG 0.599760 7.3:+85658680 MS.gene45230:CDS
GATCAAACAATAACCTGGAG+AGG 0.602921 7.3:-85659449 None:intergenic
TGAGAAAGAGAACCATGTAG+TGG 0.609227 7.3:+85658267 MS.gene45230:CDS
ATGTTTGAATGTGTTCCAGA+AGG 0.610554 7.3:+85659584 MS.gene45230:CDS
ACCATCATCATTGGTCCGAG+TGG 0.615333 7.3:-85658557 None:intergenic
AAACAATGTTGGATTTGTGT+CGG 0.622769 7.3:+85658429 MS.gene45230:CDS
ATAATCCTTGAAATATACAA+AGG 0.628176 7.3:+85659295 MS.gene45230:CDS
AAACAATAACCTGGAGAGGG+TGG 0.629227 7.3:-85659445 None:intergenic
TCAACACTGGTTGATGTAGG+AGG 0.630452 7.3:+85659331 MS.gene45230:CDS
ATCAAACAATAACCTGGAGA+GGG 0.630694 7.3:-85659448 None:intergenic
GCTTGTGTTCCATAATTGGT+CGG 0.631020 7.3:+85659895 MS.gene45230:intron
CTTTGTCCATCATGAAAACG+AGG 0.635174 7.3:+85658621 MS.gene45230:CDS
ATGATGTTTATCACAGCTGG+TGG 0.648385 7.3:+85660055 MS.gene45230:CDS
GACCAATGATGATGGTAGCA+TGG 0.656306 7.3:+85658564 MS.gene45230:CDS
CAAGCCTATTTGGTAAGTCG+GGG 0.659506 7.3:-85658489 None:intergenic
ATATGAGGGAATATCAACAC+TGG 0.673093 7.3:+85659318 MS.gene45230:CDS
TGGAAACGGACAGGAGACCG+TGG 0.690766 7.3:-85658537 None:intergenic
TGTAAAGTTCCATCACACCA+AGG 0.691403 7.3:-85660165 None:intergenic
TGGACAAAGTATTTACCAGG+AGG 0.738714 7.3:-85658607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAACTATATTAAGTTA+TGG - chr7.3:85658717-85658736 None:intergenic 10.0%
!!! AATATTGTCAAATTTTATTT+AGG + chr7.3:85659539-85659558 MS.gene45230:intron 10.0%
!!! ATATTGTCAAATTTTATTTA+GGG + chr7.3:85659540-85659559 MS.gene45230:intron 10.0%
!! AATTACAAATAATCTTATAG+TGG - chr7.3:85659047-85659066 None:intergenic 15.0%
!! ATGAATTCAAATGATAATAT+AGG + chr7.3:85658919-85658938 MS.gene45230:intron 15.0%
!! TCGAATAAATTACATATTAT+CGG - chr7.3:85659011-85659030 None:intergenic 15.0%
!! TTAATGGCATTTATATATAA+AGG - chr7.3:85659776-85659795 None:intergenic 15.0%
!!! AAAAATCATTTTTGTGATAT+AGG + chr7.3:85659122-85659141 MS.gene45230:intron 15.0%
!! AATAAGATTAAATAGTATGC+TGG + chr7.3:85658741-85658760 MS.gene45230:intron 20.0%
!! ACATTCAAAACAAATAATCT+TGG - chr7.3:85659506-85659525 None:intergenic 20.0%
!! ATAATCCTTGAAATATACAA+AGG + chr7.3:85659295-85659314 MS.gene45230:CDS 20.0%
!!! AAATTGATTGCTTTTATTGA+TGG - chr7.3:85659397-85659416 None:intergenic 20.0%
! AAATGGTAAAGTCATTATTG+TGG + chr7.3:85659967-85659986 MS.gene45230:CDS 25.0%
! AACAAATAATCTTGGAAAAG+TGG - chr7.3:85659498-85659517 None:intergenic 25.0%
! ATAATGACTTTACCATTTGA+AGG - chr7.3:85659965-85659984 None:intergenic 25.0%
! ATATCACATGAAAACAATGT+TGG + chr7.3:85658418-85658437 MS.gene45230:CDS 25.0%
! CATATCCTTTGTATATTTCA+AGG - chr7.3:85659303-85659322 None:intergenic 25.0%
! CATATTATCGGTCATAATAA+AGG - chr7.3:85658999-85659018 None:intergenic 25.0%
! CTTCAATTTCACATCATATA+TGG - chr7.3:85658889-85658908 None:intergenic 25.0%
! GAAATATACAAAGGATATGA+GGG + chr7.3:85659304-85659323 MS.gene45230:CDS 25.0%
! TATCACTATCATCTATTTGT+GGG - chr7.3:85658303-85658322 None:intergenic 25.0%
! TGAAAACGAGGATAATTATA+TGG + chr7.3:85658633-85658652 MS.gene45230:CDS 25.0%
! TGAAATATACAAAGGATATG+AGG + chr7.3:85659303-85659322 MS.gene45230:CDS 25.0%
! TGGCATTTATATATAAAGGT+TGG - chr7.3:85659772-85659791 None:intergenic 25.0%
!! AAATGATCATCTTCTTTTGA+TGG + chr7.3:85659656-85659675 MS.gene45230:intron 25.0%
!! CGAGTACAATTTTATAAGTT+TGG - chr7.3:85658945-85658964 None:intergenic 25.0%
!! TTCTCAAAGTATTCATAGAT+AGG - chr7.3:85659211-85659230 None:intergenic 25.0%
!!! GCTATTTGATCGTTTATTTT+CGG - chr7.3:85658826-85658845 None:intergenic 25.0%
!!! TGCTTTTATTGATGGATATT+TGG - chr7.3:85659389-85659408 None:intergenic 25.0%
AAACAATGTTGGATTTGTGT+CGG + chr7.3:85658429-85658448 MS.gene45230:CDS 30.0%
AGTGTCCAAGTTAAACATTT+AGG - chr7.3:85658849-85658868 None:intergenic 30.0%
ATAGCCCTAAATGTTTAACT+TGG + chr7.3:85658841-85658860 MS.gene45230:intron 30.0%
ATCACTATCATCTATTTGTG+GGG - chr7.3:85658302-85658321 None:intergenic 30.0%
CATATTGGAAGCTATAATCA+TGG - chr7.3:85658335-85658354 None:intergenic 30.0%
CATGATTATAGCTTCCAATA+TGG + chr7.3:85658333-85658352 MS.gene45230:CDS 30.0%
CTTGTTTGATATCATTGCTA+AGG + chr7.3:85658393-85658412 MS.gene45230:CDS 30.0%
GTATCACTATCATCTATTTG+TGG - chr7.3:85658304-85658323 None:intergenic 30.0%
GTGTCCAAGTTAAACATTTA+GGG - chr7.3:85658848-85658867 None:intergenic 30.0%
TAATGACGTGTAGATATAAG+TGG - chr7.3:85658784-85658803 None:intergenic 30.0%
! AATTATCCTCGTTTTCATGA+TGG - chr7.3:85658630-85658649 None:intergenic 30.0%
! ATTTTTCATCCGACCAATTA+TGG - chr7.3:85659907-85659926 None:intergenic 30.0%
!! TTTGTTGATTCAAGATCTTC+TGG - chr7.3:85660004-85660023 None:intergenic 30.0%
AACATGATGTTTATCACAGC+TGG + chr7.3:85660052-85660071 MS.gene45230:CDS 35.0%
AACGTATTCGAGAAAGTTCA+TGG + chr7.3:85659181-85659200 MS.gene45230:CDS 35.0%
AAGCTATAATCATGGCTAAG+AGG - chr7.3:85658327-85658346 None:intergenic 35.0%
AGCTATAATCATGGCTAAGA+GGG - chr7.3:85658326-85658345 None:intergenic 35.0%
ATATGAGGGAATATCAACAC+TGG + chr7.3:85659318-85659337 MS.gene45230:CDS 35.0%
ATCAAACAATAACCTGGAGA+GGG - chr7.3:85659451-85659470 None:intergenic 35.0%
ATGTTTGAATGTGTTCCAGA+AGG + chr7.3:85659584-85659603 MS.gene45230:CDS 35.0%
CGACAAACAACTTGGAATTT+AGG - chr7.3:85660133-85660152 None:intergenic 35.0%
GAGAGTTTGAGTAAACAATC+TGG + chr7.3:85660103-85660122 MS.gene45230:CDS 35.0%
GAGATGATCAAACAATAACC+TGG - chr7.3:85659457-85659476 None:intergenic 35.0%
GTGATATAGGTCGAATTTGA+AGG + chr7.3:85659135-85659154 MS.gene45230:intron 35.0%
GTTCTCTTTCTCATTGGTAT+AGG - chr7.3:85658260-85658279 None:intergenic 35.0%
TACATGGTTCTCTTTCTCAT+TGG - chr7.3:85658266-85658285 None:intergenic 35.0%
TAGACCTCAAGTTAGCAAAA+TGG - chr7.3:85659742-85659761 None:intergenic 35.0%
TATTTAGGGATCGAACATGT+TGG + chr7.3:85659554-85659573 MS.gene45230:intron 35.0%
TTCTCGAATACGTTTATCTC+TGG - chr7.3:85659175-85659194 None:intergenic 35.0%
! ATATCAACACTGGTTGATGT+AGG + chr7.3:85659328-85659347 MS.gene45230:CDS 35.0%
! TGATGGACAAAGTATTTACC+AGG - chr7.3:85658613-85658632 None:intergenic 35.0%
! TGTAGCTTGTGTTCCATAAT+TGG + chr7.3:85659891-85659910 MS.gene45230:intron 35.0%
!! ATTGCAGCTTTAAAAACTGC+AGG - chr7.3:85658364-85658383 None:intergenic 35.0%
!!! GGATTGTTATGTTTTCAGGT+TGG - chr7.3:85659715-85659734 None:intergenic 35.0%
AACTGCAGGAAACACCATAT+TGG - chr7.3:85658350-85658369 None:intergenic 40.0%
AATACCCCGACTTACCAAAT+AGG + chr7.3:85658485-85658504 MS.gene45230:CDS 40.0%
ACCTCAAGTTAGCAAAATGG+CGG - chr7.3:85659739-85659758 None:intergenic 40.0%
AGCATTATAGCATCTCCTTC+TGG - chr7.3:85659602-85659621 None:intergenic 40.0%
AGGAGATGCTATAATGCTCA+AGG + chr7.3:85659604-85659623 MS.gene45230:CDS 40.0%
ATGATGTTTATCACAGCTGG+TGG + chr7.3:85660055-85660074 MS.gene45230:CDS 40.0%
CACAAAGCTTTGCCTTCAAA+TGG + chr7.3:85659950-85659969 MS.gene45230:CDS 40.0%
CTTTGTCCATCATGAAAACG+AGG + chr7.3:85658621-85658640 MS.gene45230:CDS 40.0%
GATCAAACAATAACCTGGAG+AGG - chr7.3:85659452-85659471 None:intergenic 40.0%
GATGCTATAATGCTCAAGGT+TGG + chr7.3:85659608-85659627 MS.gene45230:intron 40.0%
GATGGTGCGTGTGTATATAT+GGG + chr7.3:85659674-85659693 MS.gene45230:intron 40.0%
GCTTGTGTTCCATAATTGGT+CGG + chr7.3:85659895-85659914 MS.gene45230:intron 40.0%
GTCAAGCCTATTTGGTAAGT+CGG - chr7.3:85658494-85658513 None:intergenic 40.0%
GTGCATGTTCAATCACTTGT+GGG - chr7.3:85659426-85659445 None:intergenic 40.0%
TCAAGCCTATTTGGTAAGTC+GGG - chr7.3:85658493-85658512 None:intergenic 40.0%
TGAGAAAGAGAACCATGTAG+TGG + chr7.3:85658267-85658286 MS.gene45230:CDS 40.0%
TGTAAAGTTCCATCACACCA+AGG - chr7.3:85660168-85660187 None:intergenic 40.0%
TTGAATCACCCAGCTTTCTT+AGG + chr7.3:85658673-85658692 MS.gene45230:CDS 40.0%
! AGAAAGCACGACAAACAACT+TGG - chr7.3:85660141-85660160 None:intergenic 40.0%
! CGGAGGATTGTTATGTTTTC+AGG - chr7.3:85659719-85659738 None:intergenic 40.0%
! TGATGGTGCGTGTGTATATA+TGG + chr7.3:85659673-85659692 MS.gene45230:intron 40.0%
! TGGACAAAGTATTTACCAGG+AGG - chr7.3:85658610-85658629 None:intergenic 40.0%
!! AAGTCGGGGTATTGAGTTAT+TGG - chr7.3:85658478-85658497 None:intergenic 40.0%
AAACAATAACCTGGAGAGGG+TGG - chr7.3:85659448-85659467 None:intergenic 45.0%
AAGTTCCATCACACCAAGGA+TGG - chr7.3:85660164-85660183 None:intergenic 45.0%
ACCACACTCCTAAGAAAGCT+GGG - chr7.3:85658684-85658703 None:intergenic 45.0%
AGCATACGGTCAAGCCTATT+TGG - chr7.3:85658502-85658521 None:intergenic 45.0%
ATCATTGGTCCGAGTGGAAA+CGG - chr7.3:85658554-85658573 None:intergenic 45.0%
CAAGCCTATTTGGTAAGTCG+GGG - chr7.3:85658492-85658511 None:intergenic 45.0%
CACCATGCTACCATCATCAT+TGG - chr7.3:85658569-85658588 None:intergenic 45.0%
GGGTTAGAGTTTCCACTACA+TGG - chr7.3:85658282-85658301 None:intergenic 45.0%
GGTGCATGTTCAATCACTTG+TGG - chr7.3:85659427-85659446 None:intergenic 45.0%
GTTGATGTAGGAGGTGGAAA+TGG + chr7.3:85659340-85659359 MS.gene45230:CDS 45.0%
GTTTATCACAGCTGGTGGTA+AGG + chr7.3:85660060-85660079 MS.gene45230:CDS 45.0%
GTTTATGCAATCACACCTCC+TGG + chr7.3:85658592-85658611 MS.gene45230:CDS 45.0%
TACCACACTCCTAAGAAAGC+TGG - chr7.3:85658685-85658704 None:intergenic 45.0%
TCAAGTTAGCAAAATGGCGG+AGG - chr7.3:85659736-85659755 None:intergenic 45.0%
TTAGGGATCGAACATGTTGG+AGG + chr7.3:85659557-85659576 MS.gene45230:intron 45.0%
! TCAACACTGGTTGATGTAGG+AGG + chr7.3:85659331-85659350 MS.gene45230:CDS 45.0%
! TTTCTCCATCCTTGGTGTGA+TGG + chr7.3:85660156-85660175 MS.gene45230:CDS 45.0%
!! GACCAATGATGATGGTAGCA+TGG + chr7.3:85658564-85658583 MS.gene45230:CDS 45.0%
!! TCCGCCATTTTGCTAACTTG+AGG + chr7.3:85659735-85659754 MS.gene45230:intron 45.0%
!!! ATATTTATGAATTTTTTTAA+TGG - chr7.3:85659792-85659811 None:intergenic 5.0%
ACCATCATCATTGGTCCGAG+TGG - chr7.3:85658560-85658579 None:intergenic 50.0%
ACCCAGCTTTCTTAGGAGTG+TGG + chr7.3:85658680-85658699 MS.gene45230:CDS 50.0%
TAGCAAGCAAGCGCAGCATA+CGG - chr7.3:85658516-85658535 None:intergenic 50.0%
TCCACTCGGACCAATGATGA+TGG + chr7.3:85658556-85658575 MS.gene45230:CDS 50.0%
TGTCGTGCTTTCTCCATCCT+TGG + chr7.3:85660148-85660167 MS.gene45230:CDS 50.0%
! ACACTGGTTGATGTAGGAGG+TGG + chr7.3:85659334-85659353 MS.gene45230:CDS 50.0%
GTCTCCTGTCCGTTTCCACT+CGG + chr7.3:85658542-85658561 MS.gene45230:CDS 55.0%
GAACATGCACCACCCTCTCC+AGG + chr7.3:85659436-85659455 MS.gene45230:CDS 60.0%
TGGAAACGGACAGGAGACCG+TGG - chr7.3:85658540-85658559 None:intergenic 60.0%
TGGTCCGAGTGGAAACGGAC+AGG - chr7.3:85658549-85658568 None:intergenic 60.0%
! CTGCGCTTGCTTGCTAGCCA+CGG + chr7.3:85658520-85658539 MS.gene45230:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 85658250 85660192 85658250 ID=MS.gene45230
chr7.3 mRNA 85658250 85660192 85658250 ID=MS.gene45230.t1;Parent=MS.gene45230
chr7.3 exon 85658250 85658701 85658250 ID=MS.gene45230.t1.exon1;Parent=MS.gene45230.t1
chr7.3 CDS 85658250 85658701 85658250 ID=cds.MS.gene45230.t1;Parent=MS.gene45230.t1
chr7.3 exon 85659144 85659457 85659144 ID=MS.gene45230.t1.exon2;Parent=MS.gene45230.t1
chr7.3 CDS 85659144 85659457 85659144 ID=cds.MS.gene45230.t1;Parent=MS.gene45230.t1
chr7.3 exon 85659561 85659625 85659561 ID=MS.gene45230.t1.exon3;Parent=MS.gene45230.t1
chr7.3 CDS 85659561 85659625 85659561 ID=cds.MS.gene45230.t1;Parent=MS.gene45230.t1
chr7.3 exon 85659896 85660192 85659896 ID=MS.gene45230.t1.exon4;Parent=MS.gene45230.t1
chr7.3 CDS 85659896 85660192 85659896 ID=cds.MS.gene45230.t1;Parent=MS.gene45230.t1
Gene Sequence

>MS.gene45230

ATGGGTTCCTATACCAATGAGAAAGAGAACCATGTAGTGGAAACTCTAACCCCACAAATAGATGATAGTGATACCCTCTTAGCCATGATTATAGCTTCCAATATGGTGTTTCCTGCAGTTTTTAAAGCTGCAATTGAACTCAACTTGTTTGATATCATTGCTAAGGAAATATCACATGAAAACAATGTTGGATTTGTGTCGGCTTTCGAAATTGCTTCAAAGTTACCAATAACTCAATACCCCGACTTACCAAATAGGCTTGACCGTATGCTGCGCTTGCTTGCTAGCCACGGTCTCCTGTCCGTTTCCACTCGGACCAATGATGATGGTAGCATGGTGAGAGTTTATGCAATCACACCTCCTGGTAAATACTTTGTCCATCATGAAAACGAGGATAATTATATGGACGCATTCACATCATATTTGAATCACCCAGCTTTCTTAGGAGTGTGGTAAGAGTTTCTCCATAACTTAATATAGTTATTTACAAAAATAAGATTAAATAGTATGCTGGTTCAAACTCGCAGTTCTCCACTTATATCTACACGTCATTAACGTGAGTGTGTTATTTGACCGAAAATAAACGATCAAATAGCCCTAAATGTTTAACTTGGACACTTGCATCAAATAATAACTCCATATATGATGTGAAATTGAAGAATCTAAATAATGAATTCAAATGATAATATAGGCCAAACTTATAAAATTGTACTCGAATCAAACTCAGTTTATATTTCATCGTTTTACCTTTATTATGACCGATAATATGTAATTTATTCGATAGCTAAAATTGACCACTATAAGATTATTTGTAATTCGAAATACATAGTCACAAAAAATAATGTCACATAATTGTTTGTATTGTTATGAACAAAAATCATTTTTGTGATATAGGTCGAATTTGAAGGAAGCAATTATAGAACCAGAGATAAACGTATTCGAGAAAGTTCATGGAATACCTATCTATGAATACTTTGAGAAAGATCAACAAATAAACAACTTATTTAACAAAGCAATGACTCAAACATGTACAATTCACATGAAAATAATCCTTGAAATATACAAAGGATATGAGGGAATATCAACACTGGTTGATGTAGGAGGTGGAAATGGACAAATTCTTAAAATGATTATATCCAAATATCCATCAATAAAAGCAATCAATTTTGATCTCCCACAAGTGATTGAACATGCACCACCCTCTCCAGGTTATTGTTTGATCATCTCAAATTTTATATTTAGTGACCACTTTTCCAAGATTATTTGTTTTGAATGTTATGAAATATTGAAATATTGTCAAATTTTATTTAGGGATCGAACATGTTGGAGGAAATATGTTTGAATGTGTTCCAGAAGGAGATGCTATAATGCTCAAGGTTGGTATACATAAATAAATATAAAAAAAAAAATGATCATCTTCTTTTGATGGTGCGTGTGTATATATGGGAGTTGCTAACTTCGACCAACCTGAAAACATAACAATCCTCCGCCATTTTGCTAACTTGAGGTCTAAGTTAGACCAACCTTTATATATAAATGCCATTAAAAAAATTCATAAATATAAACATTTATTTTATCTAATTAATTATAAAATATTGCTAATATCATCGATTCTAACTTTGTACACATGACTTATATTGTTGTAGCTTGTGTTCCATAATTGGTCGGATGAAAAATGCTTAGAAACTTTAAGCAATTGTCACAAAGCTTTGCCTTCAAATGGTAAAGTCATTATTGTGGATTTCATATCTCCAGAAGATCTTGAATCAACAAATGCATCTAAGATGATTTCAACTGTTGATAACATGATGTTTATCACAGCTGGTGGTAAGGAAAGAACTTCTAAAGAATTTGAGAGTTTGAGTAAACAATCTGGATTTCCTAAATTCCAAGTTGTTTGTCGTGCTTTCTCCATCCTTGGTGTGATGGAACTTTACAAATAA
Protein sequence

>MS.gene45230.t1

MGSYTNEKENHVVETLTPQIDDSDTLLAMIIASNMVFPAVFKAAIELNLFDIIAKEISHENNVGFVSAFEIASKLPITQYPDLPNRLDRMLRLLASHGLLSVSTRTNDDGSMVRVYAITPPGKYFVHHENEDNYMDAFTSYLNHPAFLGVWSNLKEAIIEPEINVFEKVHGIPIYEYFEKDQQINNLFNKAMTQTCTIHMKIILEIYKGYEGISTLVDVGGGNGQILKMIISKYPSIKAINFDLPQVIEHAPPSPGIEHVGGNMFECVPEGDAIMLKLVFHNWSDEKCLETLSNCHKALPSNGKVIIVDFISPEDLESTNASKMISTVDNMMFITAGGKERTSKEFESLSKQSGFPKFQVVCRAFSILGVMELYK