AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058177


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058177.t1 MTR_2g035130 96.178 157 6 0 1 157 1 157 3.47e-106 300
MS.gene058177.t1 MTR_2g035150 85.443 158 23 0 1 158 1 158 6.03e-96 274
MS.gene058177.t1 MTR_2g035100 87.261 157 20 0 1 157 1 157 4.20e-95 272
MS.gene058177.t1 MTR_2g035120 85.443 158 23 0 1 158 1 158 6.04e-94 269
MS.gene058177.t1 MTR_2g035105 85.987 157 22 0 1 157 1 157 1.01e-93 268
MS.gene058177.t1 MTR_6g033450 68.354 158 50 0 1 158 1 158 9.74e-77 225
MS.gene058177.t1 MTR_4g120970 68.354 158 50 0 1 158 1 158 9.74e-77 225
MS.gene058177.t1 MTR_2g035320 60.510 157 62 0 1 157 1 157 1.22e-63 192
MS.gene058177.t1 MTR_2g035190 58.599 157 65 0 1 157 1 157 1.67e-61 187
MS.gene058177.t1 MTR_2g035210 57.325 157 67 0 1 157 1 157 1.78e-59 182
MS.gene058177.t1 MTR_2g035220 57.325 157 67 0 1 157 1 157 2.65e-58 179
MS.gene058177.t1 MTR_2g035170 56.051 157 69 0 1 157 1 157 6.72e-58 177
MS.gene058177.t1 MTR_2g435310 38.462 156 95 1 1 155 1 156 2.68e-37 125
MS.gene058177.t1 MTR_4g120760 38.854 157 95 1 1 156 1 157 4.24e-36 122
MS.gene058177.t1 MTR_4g120760 38.854 157 95 1 1 156 1 157 4.58e-36 122
MS.gene058177.t1 MTR_2g035320 57.576 99 42 0 59 157 24 122 4.72e-35 119
MS.gene058177.t1 MTR_4g120950 44.444 162 83 5 1 158 1 159 1.99e-32 113
MS.gene058177.t1 MTR_4g120940 61.798 89 31 1 70 158 35 120 1.26e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGCATAGTTGGTGGTTT+TGG 0.149266 2.1:-57694842 MS.gene058177:CDS
TGATGGCATCAATAACCTTT+GGG 0.301288 2.1:+57695745 None:intergenic
AACGATATCAATACTCTTGA+TGG 0.325047 2.1:+57695728 None:intergenic
TTTGCAATGGTTCCTCCATT+TGG 0.327211 2.1:+57694767 None:intergenic
TATATGCTTTAGTTGTAATC+AGG 0.341112 2.1:+57694626 None:intergenic
GAAATCTTCTCCACTGTGTC+TGG 0.343745 2.1:+57694815 None:intergenic
TTGATGGCATCAATAACCTT+TGG 0.345179 2.1:+57695744 None:intergenic
GGCTCTTGAAGGTTATGTTT+TGG 0.350667 2.1:-57694654 MS.gene058177:CDS
CACTTTGTGCAACACATACT+TGG 0.384404 2.1:+57694898 None:intergenic
CTTAGTAGATGATGTTAACT+TGG 0.391360 2.1:-57694873 MS.gene058177:CDS
CATCAATAACCTTTGGGGTA+AGG 0.433870 2.1:+57695751 None:intergenic
ACTAGGTGCAAAATCACCTT+TGG 0.438881 2.1:+57694724 None:intergenic
AAGAGTATTGATATCGTTGA+AGG 0.465901 2.1:-57695723 MS.gene058177:CDS
GCTTACCACTACAGCATAGT+TGG 0.468258 2.1:-57694851 MS.gene058177:CDS
AGTGGAGAAGATTTCATTTG+AGG 0.476098 2.1:-57694807 MS.gene058177:CDS
AAATTGTCTGCAGGACCAAA+TGG 0.477800 2.1:-57694782 MS.gene058177:CDS
AGAGCTTTGTAAAGTGTAGC+AGG 0.480318 2.1:+57695792 None:intergenic
TTTGAGGCTAAATTGTCTGC+AGG 0.494016 2.1:-57694791 MS.gene058177:CDS
AATGGCAAAGCTAGGGGTGA+TGG 0.523022 2.1:-57694686 MS.gene058177:CDS
TTCTTGAGTTCCTCTTCACT+AGG 0.524188 2.1:+57694707 None:intergenic
CAAGTATGTGTTGCACAAAG+TGG 0.538293 2.1:-57694897 MS.gene058177:CDS
AACTCAAGAATGGCAAAGCT+AGG 0.540466 2.1:-57694694 MS.gene058177:CDS
ACTCAAGAATGGCAAAGCTA+GGG 0.556431 2.1:-57694693 MS.gene058177:CDS
ATTGATATCGTTGAAGGAGA+CGG 0.559020 2.1:-57695717 MS.gene058177:CDS
TGTAGCAGGAGCTACAATAG+AGG 0.565152 2.1:+57695806 None:intergenic
TACCACTACAGCATAGTTGG+TGG 0.567411 2.1:-57694848 MS.gene058177:CDS
TTTCACACTAAGCTTTGCAA+TGG 0.568267 2.1:+57694754 None:intergenic
GATGGCATCAATAACCTTTG+GGG 0.578696 2.1:+57695746 None:intergenic
AGTGAAGAGGAACTCAAGAA+TGG 0.584353 2.1:-57694704 MS.gene058177:CDS
GTTGAAGGAGACGGTTGCGC+CGG 0.587773 2.1:-57695708 MS.gene058177:CDS
AACCACCAACTATGCTGTAG+TGG 0.607193 2.1:+57694846 None:intergenic
TGCTGACAACCTTACCCCAA+AGG 0.621335 2.1:-57695760 MS.gene058177:CDS
CTCAAGAATGGCAAAGCTAG+GGG 0.641253 2.1:-57694692 MS.gene058177:CDS
AGTGTGAAATACTTCACCAA+AGG 0.651010 2.1:-57694740 MS.gene058177:CDS
AGCAGGAGCTACAATAGAGG+TGG 0.665165 2.1:+57695809 None:intergenic
TTGTCTGCAGGACCAAATGG+AGG 0.666387 2.1:-57694779 MS.gene058177:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATATATAAAATAAT+AGG - chr2.1:57695515-57695534 MS.gene058177:intron 0.0%
!! ATCATTACTAGTATTATATA+AGG - chr2.1:57695475-57695494 MS.gene058177:intron 15.0%
!! GTGATAAAAAAATTGATAAA+CGG + chr2.1:57695044-57695063 None:intergenic 15.0%
!! ACTCTTATGATTAACATATT+TGG - chr2.1:57694953-57694972 MS.gene058177:intron 20.0%
!! ATATTTGGTTGCTAATTAAT+TGG - chr2.1:57694968-57694987 MS.gene058177:intron 20.0%
! AAAATTGATAAACGGTAAAG+TGG + chr2.1:57695036-57695055 None:intergenic 25.0%
! AACTTACTTTAATACAACCT+TGG + chr2.1:57695164-57695183 None:intergenic 25.0%
! CTTTCAAATATACCGTTAAA+AGG + chr2.1:57695274-57695293 None:intergenic 25.0%
! TTAGTCAAAGACAAATTGTT+CGG + chr2.1:57695392-57695411 None:intergenic 25.0%
!! AGAATGCTAGAATATTCTTT+TGG - chr2.1:57695292-57695311 MS.gene058177:intron 25.0%
!!! TGATTTGGTATACTTGAAAA+AGG - chr2.1:57695112-57695131 MS.gene058177:intron 25.0%
AACACAATTGCTTGATTAAC+TGG - chr2.1:57695429-57695448 MS.gene058177:intron 30.0%
AACGATATCAATACTCTTGA+TGG + chr2.1:57694746-57694765 None:intergenic 30.0%
CAAAATGGACAATTTAACCA+AGG - chr2.1:57695144-57695163 MS.gene058177:intron 30.0%
CTTAGTAGATGATGTTAACT+TGG - chr2.1:57695598-57695617 MS.gene058177:intron 30.0%
CTTAGTGATCTCATTCTTTA+TGG - chr2.1:57695238-57695257 MS.gene058177:intron 30.0%
GATCTCATTCTTTATGGAAT+AGG - chr2.1:57695244-57695263 MS.gene058177:intron 30.0%
TAAAGAATGAGATCACTAAG+CGG + chr2.1:57695238-57695257 None:intergenic 30.0%
TAGTCAAAGACAAATTGTTC+GGG + chr2.1:57695391-57695410 None:intergenic 30.0%
TCAAGTATACCAAATCAATG+TGG + chr2.1:57695109-57695128 None:intergenic 30.0%
! ATCAAAGTGTCACAATTACT+AGG + chr2.1:57695070-57695089 None:intergenic 30.0%
! GAAGAATAACCACATTGATT+TGG - chr2.1:57695097-57695116 MS.gene058177:intron 30.0%
! GACAAAAGTGATTTTCTTGA+TGG + chr2.1:57694791-57694810 None:intergenic 30.0%
! TAAATTGTCCATTTTGTGCT+AGG + chr2.1:57695140-57695159 None:intergenic 30.0%
!! AAGAAAATCACTTTTGTCGA+AGG - chr2.1:57694793-57694812 MS.gene058177:CDS 30.0%
!! AAGAGTATTGATATCGTTGA+AGG - chr2.1:57694748-57694767 MS.gene058177:CDS 30.0%
AAAAGGTTCCTAGCACAAAA+TGG - chr2.1:57695129-57695148 MS.gene058177:intron 35.0%
AGTGGAGAAGATTTCATTTG+AGG - chr2.1:57695664-57695683 MS.gene058177:intron 35.0%
AGTGTGAAATACTTCACCAA+AGG - chr2.1:57695731-57695750 MS.gene058177:CDS 35.0%
ATCTAACTGCGAAATTCACA+CGG - chr2.1:57694859-57694878 MS.gene058177:CDS 35.0%
ATTGATATCGTTGAAGGAGA+CGG - chr2.1:57694754-57694773 MS.gene058177:CDS 35.0%
TGATGGCATCAATAACCTTT+GGG + chr2.1:57694729-57694748 None:intergenic 35.0%
TTCTAATCTGAGTATCAGTC+AGG - chr2.1:57695328-57695347 MS.gene058177:intron 35.0%
TTTAACCAATGATGTGCAGA+TGG - chr2.1:57695545-57695564 MS.gene058177:intron 35.0%
TTTCACACTAAGCTTTGCAA+TGG + chr2.1:57695720-57695739 None:intergenic 35.0%
! GGAATAGGTCATCCTTTTAA+CGG - chr2.1:57695259-57695278 MS.gene058177:intron 35.0%
! TCTGCACATCATTGGTTAAA+AGG + chr2.1:57695545-57695564 None:intergenic 35.0%
! TTGATGGCATCAATAACCTT+TGG + chr2.1:57694730-57694749 None:intergenic 35.0%
!! AGTGATTTTCTTGATGGTTC+CGG + chr2.1:57694785-57694804 None:intergenic 35.0%
AAATTGTCTGCAGGACCAAA+TGG - chr2.1:57695689-57695708 MS.gene058177:CDS 40.0%
AACTCAAGAATGGCAAAGCT+AGG - chr2.1:57695777-57695796 MS.gene058177:CDS 40.0%
ACTAGGTGCAAAATCACCTT+TGG + chr2.1:57695750-57695769 None:intergenic 40.0%
ACTCAAGAATGGCAAAGCTA+GGG - chr2.1:57695778-57695797 MS.gene058177:CDS 40.0%
AGTGAAGAGGAACTCAAGAA+TGG - chr2.1:57695767-57695786 MS.gene058177:CDS 40.0%
CAAGTATGTGTTGCACAAAG+TGG - chr2.1:57695574-57695593 MS.gene058177:intron 40.0%
CACTTTGTGCAACACATACT+TGG + chr2.1:57695576-57695595 None:intergenic 40.0%
GATGGCATCAATAACCTTTG+GGG + chr2.1:57694728-57694747 None:intergenic 40.0%
TTCTTGAGTTCCTCTTCACT+AGG + chr2.1:57695767-57695786 None:intergenic 40.0%
TTTCACCATCTGCACATCAT+TGG + chr2.1:57695553-57695572 None:intergenic 40.0%
TTTGCAATGGTTCCTCCATT+TGG + chr2.1:57695707-57695726 None:intergenic 40.0%
! AGAGCTTTGTAAAGTGTAGC+AGG + chr2.1:57694682-57694701 None:intergenic 40.0%
! GGCTCTTGAAGGTTATGTTT+TGG - chr2.1:57695817-57695836 MS.gene058177:CDS 40.0%
! TGATTTTGCACCTAGTGAAG+AGG - chr2.1:57695754-57695773 MS.gene058177:CDS 40.0%
! TTTGAGGCTAAATTGTCTGC+AGG - chr2.1:57695680-57695699 MS.gene058177:CDS 40.0%
!! CATCAATAACCTTTGGGGTA+AGG + chr2.1:57694723-57694742 None:intergenic 40.0%
!!! ACAGCATAGTTGGTGGTTTT+GGG - chr2.1:57695630-57695649 MS.gene058177:intron 40.0%
!!! TACAGCATAGTTGGTGGTTT+TGG - chr2.1:57695629-57695648 MS.gene058177:intron 40.0%
AACCACCAACTATGCTGTAG+TGG + chr2.1:57695628-57695647 None:intergenic 45.0%
CTCAAGAATGGCAAAGCTAG+GGG - chr2.1:57695779-57695798 MS.gene058177:CDS 45.0%
GAAATCTTCTCCACTGTGTC+TGG + chr2.1:57695659-57695678 None:intergenic 45.0%
GCTTACCACTACAGCATAGT+TGG - chr2.1:57695620-57695639 MS.gene058177:intron 45.0%
TACCACTACAGCATAGTTGG+TGG - chr2.1:57695623-57695642 MS.gene058177:intron 45.0%
TGTAGCAGGAGCTACAATAG+AGG + chr2.1:57694668-57694687 None:intergenic 45.0%
!! GGTCTTTTCAAGGCTCTTGA+AGG - chr2.1:57695806-57695825 MS.gene058177:CDS 45.0%
!! TAGGGGTGATGGTCTTTTCA+AGG - chr2.1:57695796-57695815 MS.gene058177:CDS 45.0%
AATGGCAAAGCTAGGGGTGA+TGG - chr2.1:57695785-57695804 MS.gene058177:CDS 50.0%
AGCAGGAGCTACAATAGAGG+TGG + chr2.1:57694665-57694684 None:intergenic 50.0%
TGCTGACAACCTTACCCCAA+AGG - chr2.1:57694711-57694730 MS.gene058177:CDS 50.0%
TTGTCTGCAGGACCAAATGG+AGG - chr2.1:57695692-57695711 MS.gene058177:CDS 50.0%
! TTTTGGGCTTCCAGACACAG+TGG - chr2.1:57695646-57695665 MS.gene058177:intron 50.0%
GTTGAAGGAGACGGTTGCGC+CGG - chr2.1:57694763-57694782 MS.gene058177:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 57694634 57695859 57694634 ID=MS.gene058177
chr2.1 mRNA 57694634 57695859 57694634 ID=MS.gene058177.t1;Parent=MS.gene058177
chr2.1 exon 57695679 57695859 57695679 ID=MS.gene058177.t1.exon1;Parent=MS.gene058177.t1
chr2.1 CDS 57695679 57695859 57695679 ID=cds.MS.gene058177.t1;Parent=MS.gene058177.t1
chr2.1 exon 57694634 57694929 57694634 ID=MS.gene058177.t1.exon2;Parent=MS.gene058177.t1
chr2.1 CDS 57694634 57694929 57694634 ID=cds.MS.gene058177.t1;Parent=MS.gene058177.t1
Gene Sequence

>MS.gene058177

ATGGGTGTTTTCAACTTTGAGGATGAAACCACCTCTATTGTAGCTCCTGCTACACTTTACAAAGCTCTTGTTACAGATGCTGACAACCTTACCCCAAAGGTTATTGATGCCATCAAGAGTATTGATATCGTTGAAGGAGACGGTTGCGCCGGAACCATCAAGAAAATCACTTTTGTCGAAGGTCAGTATAAATATATGTGTGTATTTTTTTGATTATTATAAAGAATCTAACTGCGAAATTCACACGGATTAAGAGAAAAATTGATTCACTATACAGATAAAATACAATTTATAAAATATATGAGAGCGTTTAGTAGACACTCTTATGATTAACATATTTGGTTGCTAATTAATTGGTTTGAGAAAATATTTTCTATTTTTACTTTAATTATAGAATTACCACTTTACCGTTTATCAATTTTTTTATCACTTTCCTAGTAATTGTGACACTTTGATGTTAGTAGAAGAATAACCACATTGATTTGGTATACTTGAAAAAGGTTCCTAGCACAAAATGGACAATTTAACCAAGGTTGTATTAAAGTAAGTTTATACTTAAAAAGATAAAATAAAATGAAGTTCTTATAAGAAAAGTAAATTTCCGCTTAGTGATCTCATTCTTTATGGAATAGGTCATCCTTTTAACGGTATATTTGAAAGAATGCTAGAATATTCTTTTGGTGATGTTATTTTGTTCTAATCTGAGTATCAGTCAGGATATAAGTATAGTTTAGTCAAAATTATCTCAAATTCTCCCGAACAATTTGTCTTTGACTAAAATCAATTAACTATTTAAACACAATTGCTTGATTAACTGGAACTTGATTATTTTATTGATGATATCATTACTAGTATTATATAAGGATTGTTCATTAGTTTGTTTTTTTATATATAAAATAATAGGTCATCCTTTTAACCAATGATGTGCAGATGGTGAAACCAAGTATGTGTTGCACAAAGTGGACTTAGTAGATGATGTTAACTTGGCTTACCACTACAGCATAGTTGGTGGTTTTGGGCTTCCAGACACAGTGGAGAAGATTTCATTTGAGGCTAAATTGTCTGCAGGACCAAATGGAGGAACCATTGCAAAGCTTAGTGTGAAATACTTCACCAAAGGTGATTTTGCACCTAGTGAAGAGGAACTCAAGAATGGCAAAGCTAGGGGTGATGGTCTTTTCAAGGCTCTTGAAGGTTATGTTTTGGCAAATCCTGATTACAACTAA

Protein sequence

>MS.gene058177.t1

MGVFNFEDETTSIVAPATLYKALVTDADNLTPKVIDAIKSIDIVEGDGCAGTIKKITFVEDGETKYVLHKVDLVDDVNLAYHYSIVGGFGLPDTVEKISFEAKLSAGPNGGTIAKLSVKYFTKGDFAPSEEELKNGKARGDGLFKALEGYVLANPDYN