AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46480


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46480.t1 MTR_6g033375 96.154 182 7 0 1 182 157 338 1.33e-124 354
MS.gene46480.t1 MTR_6g045290 90.000 160 14 1 1 158 131 290 5.93e-84 256
MS.gene46480.t1 MTR_8g070660 55.030 169 75 1 1 168 182 350 1.06e-53 174
MS.gene46480.t1 MTR_5g069490 46.980 149 65 1 1 149 187 321 2.55e-34 124
MS.gene46480.t1 MTR_2g437500 44.167 120 67 0 36 155 1 120 4.48e-30 107
MS.gene46480.t1 MTR_2g437920 42.690 171 83 3 1 164 220 382 1.25e-28 109
MS.gene46480.t1 MTR_8g070660 59.211 76 31 0 1 76 182 257 4.14e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46480.t1 AT2G34900 52.105 190 81 2 1 180 86 275 1.06e-59 187
MS.gene46480.t1 AT2G34900 52.105 190 81 2 1 180 196 385 1.24e-58 188
MS.gene46480.t1 AT3G52280 58.042 143 60 0 1 143 200 342 2.41e-44 151
MS.gene46480.t1 AT3G52280 58.042 143 60 0 1 143 183 325 2.67e-44 150

Find 0 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAAAGTTTAATCTCCACA+AGG + 10858:42939-42958 None:intergenic 25.0%
!!! GAGATTAAACTTTTTTGTCA+AGG - 10858:42943-42962 MS.gene46480:CDS 25.0%
AACTTTACTGGAAAAGTTTG+AGG - 10858:42556-42575 MS.gene46480:CDS 30.0%
!! TTGGAAGTTTGGATTAGTTT+GGG + 10858:42867-42886 None:intergenic 30.0%
!!! ATTTTTTAACAACCATCTCC+CGG + 10858:42751-42770 None:intergenic 30.0%
ACTGAAGAGAAGAGAAATCT+TGG - 10858:42785-42804 MS.gene46480:CDS 35.0%
ATGTACATTTAGAAGAGCTC+CGG - 10858:42729-42748 MS.gene46480:CDS 35.0%
TGGAAAAGTTTGAGGAGAAA+TGG - 10858:42564-42583 MS.gene46480:CDS 35.0%
! AAGAATTCAGAAAGCATGGA+TGG - 10858:42986-43005 MS.gene46480:CDS 35.0%
!! CTTGGAAGTTTGGATTAGTT+TGG + 10858:42868-42887 None:intergenic 35.0%
!!! CTTTTCTTCGTTTGTTGTTC+AGG + 10858:43021-43040 None:intergenic 35.0%
AGAAGCAGCTCATGCTAAAA+TGG - 10858:42673-42692 MS.gene46480:CDS 40.0%
GAGCAAGAATTCAGAAAGCA+TGG - 10858:42982-43001 MS.gene46480:CDS 40.0%
TAGAGACTTGAGTAATGAGC+TGG - 10858:42697-42716 MS.gene46480:CDS 40.0%
TGCTTCTTGAGCAAATTGCA+TGG + 10858:42660-42679 None:intergenic 40.0%
TGCTTTCTGAATTCTTGCTC+TGG + 10858:42982-43001 None:intergenic 40.0%
TGTACATTTAGAAGAGCTCC+GGG - 10858:42730-42749 MS.gene46480:CDS 40.0%
! TGACGATCTTAGAAGAGCAT+TGG - 10858:42832-42851 MS.gene46480:CDS 40.0%
!! AATGCTCTTCTAAGATCGTC+AGG + 10858:42833-42852 None:intergenic 40.0%
AAGAGGAAGAAGCTGAAGCA+CGG - 10858:42630-42649 MS.gene46480:CDS 45.0%
AAGATCGTCAGGAGACAGTT+TGG + 10858:42822-42841 None:intergenic 45.0%
AGAGGAAACTAGACGAGAAG+AGG - 10858:42613-42632 MS.gene46480:CDS 45.0%
GTTTCCTCTTCAGCAACTCT+AGG + 10858:42602-42621 None:intergenic 45.0%
TCAGCATTCGCTTGGAAGTT+TGG + 10858:42878-42897 None:intergenic 45.0%
CTCAGAGTGAGTCTACCTTG+TGG - 10858:42921-42940 MS.gene46480:CDS 50.0%
TCTGCCTAGAGTTGCTGAAG+AGG - 10858:42595-42614 MS.gene46480:CDS 50.0%
TTTAGAAGAGCTCCGGGAGA+TGG - 10858:42736-42755 MS.gene46480:CDS 50.0%
CTTCCAAGCGAATGCTGACG+AGG - 10858:42880-42899 MS.gene46480:CDS 55.0%
CCAAGCGAATGCTGACGAGG+TGG - 10858:42883-42902 MS.gene46480:CDS 60.0%
CCACCTCGTCAGCATTCGCT+TGG + 10858:42886-42905 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
10858 gene 42548 43096 42548 ID=MS.gene46480
10858 mRNA 42548 43096 42548 ID=MS.gene46480.t1;Parent=MS.gene46480
10858 exon 42548 43096 42548 ID=MS.gene46480.t1.exon1;Parent=MS.gene46480.t1
10858 CDS 42548 43096 42548 ID=cds.MS.gene46480.t1;Parent=MS.gene46480.t1
Gene Sequence

>MS.gene46480

ATGGCAAAAACTTTACTGGAAAAGTTTGAGGAGAAATGGTTGCAATTTCTGCCTAGAGTTGCTGAAGAGGAAACTAGACGAGAAGAGGAAGAAGCTGAAGCACGGTTAGCCATGCAATTTGCTCAAGAAGCAGCTCATGCTAAAATGGCTAGAGACTTGAGTAATGAGCTGGATGAAGTTGATGTACATTTAGAAGAGCTCCGGGAGATGGTTGTTAAAAAATGCAGAAAAATGTCAACTGAAGAGAAGAGAAATCTTGGAATTGCTCTCACCAAACTGTCTCCTGACGATCTTAGAAGAGCATTGGATATTGTTGCCCAAACTAATCCAAACTTCCAAGCGAATGCTGACGAGGTGGATCTTGACATCGATGCTCAGAGTGAGTCTACCTTGTGGAGATTAAACTTTTTTGTCAAGGATGCTCTTGAAGTCCAGAGCAAGAATTCAGAAAGCATGGATGGCGATGAAAACCTGAACAACAAACGAAGAAAAGAATTATGCGATGCTATTGCAAATGTTTCGAAGAAGAAGACCAAGAAGCCTACTTGA

Protein sequence

>MS.gene46480.t1

MAKTLLEKFEEKWLQFLPRVAEEETRREEEEAEARLAMQFAQEAAHAKMARDLSNELDEVDVHLEELREMVVKKCRKMSTEEKRNLGIALTKLSPDDLRRALDIVAQTNPNFQANADEVDLDIDAQSESTLWRLNFFVKDALEVQSKNSESMDGDENLNNKRRKELCDAIANVSKKKTKKPT