Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051574.t1 | XP_024631073.1 | 95.1 | 411 | 20 | 0 | 1 | 411 | 7 | 417 | 1.20E-226 | 795.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051574.t1 | Q8BVH9 | 43.7 | 254 | 138 | 2 | 10 | 259 | 14 | 266 | 4.5e-52 | 206.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051574.t1 | A0A396JIH7 | 95.1 | 411 | 20 | 0 | 1 | 411 | 7 | 417 | 8.9e-227 | 795.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene051574 | 0.813898 | 2.03E-51 | -1.69E-46 |
| MS.gene051332 | MS.gene051574 | 0.815974 | 7.02E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene051744 | 0.80736 | 5.25E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene052519 | 0.802561 | 5.29E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene052582 | 0.834951 | 2.23E-56 | -1.69E-46 |
| MS.gene051574 | MS.gene052584 | 0.83552 | 1.60E-56 | -1.69E-46 |
| MS.gene051574 | MS.gene053226 | 0.822312 | 2.53E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene054651 | 0.80457 | 2.03E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene055227 | 0.801917 | 7.18E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene056050 | 0.826486 | 2.64E-54 | -1.69E-46 |
| MS.gene051574 | MS.gene056366 | 0.805176 | 1.51E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene057208 | 0.800438 | 1.44E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene058548 | 0.806312 | 8.74E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene05954 | 0.821923 | 3.12E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene059935 | 0.805454 | 1.32E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene061594 | 0.800918 | 1.15E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene062576 | 0.80501 | 1.64E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene062672 | 0.805587 | 1.24E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene06269 | 0.805768 | 1.14E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene062743 | 0.813132 | 2.99E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene062756 | 0.801583 | 8.41E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene065039 | 0.803634 | 3.18E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene066194 | 0.818647 | 1.76E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene066335 | 0.814459 | 1.52E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene067059 | 0.848294 | 6.72E-60 | -1.69E-46 |
| MS.gene051574 | MS.gene06857 | 0.800118 | 1.67E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene068924 | 0.805621 | 1.22E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene070818 | 0.83993 | 1.18E-57 | -1.69E-46 |
| MS.gene051574 | MS.gene071322 | 0.81127 | 7.62E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene071570 | 0.806605 | 7.58E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene072280 | 0.80832 | 3.28E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene072668 | 0.80003 | 1.75E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene073139 | 0.800642 | 1.31E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene073753 | 0.812326 | 4.48E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene07566 | -0.805244 | 1.47E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene08853 | 0.83052 | 2.80E-55 | -1.69E-46 |
| MS.gene051574 | MS.gene08882 | 0.803069 | 4.16E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene09600 | 0.808119 | 3.62E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene20298 | 0.805813 | 1.11E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene21700 | 0.826412 | 2.75E-54 | -1.69E-46 |
| MS.gene051574 | MS.gene21927 | 0.804996 | 1.65E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene21929 | 0.807771 | 4.30E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene22255 | 0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene22270 | 0.813154 | 2.95E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene22271 | 0.804179 | 2.45E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene23055 | 0.809102 | 2.23E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene23525 | 0.801603 | 8.33E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene23528 | 0.803721 | 3.05E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene24493 | 0.837203 | 5.97E-57 | -1.69E-46 |
| MS.gene051574 | MS.gene25207 | 0.816137 | 6.46E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene28652 | 0.825093 | 5.66E-54 | -1.69E-46 |
| MS.gene051574 | MS.gene31142 | 0.809416 | 1.91E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene31283 | 0.822987 | 1.76E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene32229 | 0.829577 | 4.76E-55 | -1.69E-46 |
| MS.gene051574 | MS.gene32231 | 0.800718 | 1.26E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene32556 | 0.800883 | 1.17E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene33624 | 0.80524 | 1.47E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene33650 | 0.806708 | 7.21E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene33651 | 0.805381 | 1.37E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene33853 | 0.818786 | 1.63E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene35044 | 0.817093 | 3.94E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene37612 | 0.819896 | 9.13E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene41235 | 0.829184 | 5.93E-55 | -1.69E-46 |
| MS.gene051574 | MS.gene43973 | 0.805059 | 1.60E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene45507 | 0.816478 | 5.42E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene46132 | 0.800695 | 1.28E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene46480 | 0.807978 | 3.88E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene47878 | 0.803379 | 3.59E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene48389 | 0.826077 | 3.31E-54 | -1.69E-46 |
| MS.gene051574 | MS.gene50615 | 0.80725 | 5.54E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene51818 | 0.804816 | 1.80E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene52061 | 0.8229 | 1.85E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene56468 | 0.810407 | 1.17E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene56868 | 0.83734 | 5.51E-57 | -1.69E-46 |
| MS.gene051574 | MS.gene56924 | 0.815971 | 7.03E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene58547 | 0.814545 | 1.46E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene58802 | 0.801287 | 9.67E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene59369 | 0.810187 | 1.31E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene59540 | 0.810747 | 9.88E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene60357 | 0.820376 | 7.09E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene60710 | 0.8117 | 6.14E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene63988 | 0.844069 | 9.50E-59 | -1.69E-46 |
| MS.gene051574 | MS.gene64249 | 0.800891 | 1.16E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene65896 | 0.801283 | 9.69E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene67662 | 0.802772 | 4.79E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene68198 | 0.805721 | 1.16E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene68999 | 0.822609 | 2.16E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene69116 | 0.80043 | 1.45E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene70534 | 0.804424 | 2.18E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene72008 | 0.812895 | 3.37E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene72268 | 0.800876 | 1.17E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene73250 | 0.803159 | 3.98E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene73467 | 0.815591 | 8.55E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene74869 | 0.801539 | 8.58E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene75963 | 0.804722 | 1.88E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene76148 | 0.829944 | 3.88E-55 | -1.69E-46 |
| MS.gene051574 | MS.gene76244 | 0.806338 | 8.63E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene77371 | 0.805214 | 1.49E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene78951 | 0.82341 | 1.41E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene79253 | 0.81132 | 7.43E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene81047 | 0.819217 | 1.30E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene82701 | 0.803486 | 3.41E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene83091 | 0.829897 | 3.98E-55 | -1.69E-46 |
| MS.gene051574 | MS.gene83734 | 0.823278 | 1.51E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene88437 | -0.803854 | 2.86E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene89277 | 0.80433 | 2.28E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene91310 | 0.811342 | 7.34E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene91676 | 0.800039 | 1.74E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene91907 | 0.805118 | 1.56E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene92059 | 0.808589 | 2.88E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene92642 | 0.801783 | 7.65E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene93764 | 0.809402 | 1.93E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene95472 | 0.817804 | 2.73E-52 | -1.69E-46 |
| MS.gene051574 | MS.gene95762 | 0.80254 | 5.35E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene96511 | -0.821035 | 5.00E-53 | -1.69E-46 |
| MS.gene051574 | MS.gene97766 | 0.80511 | 1.56E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene98953 | 0.848681 | 5.25E-60 | -1.69E-46 |
| MS.gene051574 | MS.gene99821 | 0.804225 | 2.39E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041599 | MS.gene051574 | PPI |
| MS.gene035363 | MS.gene051574 | PPI |
| MS.gene77822 | MS.gene051574 | PPI |
| MS.gene035362 | MS.gene051574 | PPI |
| MS.gene78481 | MS.gene051574 | PPI |
| MS.gene051574 | MS.gene041598 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051574.t1 | MTR_1g015770 | 91.388 | 418 | 29 | 1 | 1 | 411 | 7 | 424 | 0.0 | 791 |
| MS.gene051574.t1 | MTR_1g079800 | 32.593 | 270 | 130 | 7 | 41 | 259 | 53 | 321 | 8.14e-32 | 123 |
| MS.gene051574.t1 | MTR_1g079800 | 31.907 | 257 | 124 | 6 | 53 | 259 | 89 | 344 | 5.98e-28 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051574.t1 | AT2G26200 | 68.274 | 394 | 120 | 3 | 13 | 402 | 16 | 408 | 0.0 | 557 |
| MS.gene051574.t1 | AT2G26200 | 65.231 | 325 | 102 | 4 | 1 | 316 | 1 | 323 | 3.58e-151 | 432 |
| MS.gene051574.t1 | AT1G54650 | 34.127 | 252 | 141 | 4 | 32 | 259 | 43 | 293 | 5.02e-36 | 134 |
| MS.gene051574.t1 | AT1G54650 | 34.127 | 252 | 141 | 4 | 32 | 259 | 63 | 313 | 8.71e-36 | 134 |
| MS.gene051574.t1 | AT1G54650 | 34.906 | 106 | 66 | 1 | 32 | 134 | 63 | 168 | 6.96e-13 | 67.8 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGATGCATGAAGACTTTA+AGG | 0.140989 | 1.3:-6409162 | MS.gene051574:intron |
| AGGAGGAAAGAAAGTAATTT+TGG | 0.191683 | 1.3:-6410406 | MS.gene051574:CDS |
| ATTTATTCCAAACCAAATTC+TGG | 0.197214 | 1.3:-6412314 | MS.gene051574:CDS |
| ACAAATTTATTCAAAGAAAA+TGG | 0.213631 | 1.3:-6406762 | MS.gene051574:CDS |
| AAGGGTGAGACCCCAGAATT+TGG | 0.265459 | 1.3:+6412302 | None:intergenic |
| ATTGGCCTCGGCTTCTTTAC+TGG | 0.272625 | 1.3:+6406518 | None:intergenic |
| AAAGAGAAGCTAAGAAATAT+TGG | 0.275974 | 1.3:-6412168 | MS.gene051574:CDS |
| TCTTAAAATTCCAGCCTCTA+AGG | 0.289443 | 1.3:+6405680 | None:intergenic |
| ATTCTGGGGTCTCACCCTTT+TGG | 0.312133 | 1.3:-6412298 | MS.gene051574:CDS |
| AATCAACTGGTTTGATGCTC+TGG | 0.316619 | 1.3:-6405621 | MS.gene051574:CDS |
| TGATGCTATCACAGGAAATA+TGG | 0.318795 | 1.3:+6409880 | None:intergenic |
| CTGGGAATCGGCCCGATTAA+TGG | 0.321936 | 1.3:-6405602 | MS.gene051574:CDS |
| CATCAAACCAGTTGATTTGC+AGG | 0.327568 | 1.3:+6405627 | None:intergenic |
| CTTGATGGTGATAACAATAT+TGG | 0.341012 | 1.3:-6406492 | MS.gene051574:CDS |
| GATCTCACGGCAATGGCATC+AGG | 0.356294 | 1.3:-6412374 | None:intergenic |
| CAAGGACAGAAGCCATTAAT+CGG | 0.358894 | 1.3:+6405590 | None:intergenic |
| GAAAACCAACCTTGTCTTTG+TGG | 0.388682 | 1.3:+6412128 | None:intergenic |
| TTTCTGTAGGAGAGATTCTC+TGG | 0.398053 | 1.3:-6406953 | MS.gene051574:intron |
| TTGACATGTCTGAGGGAGTC+GGG | 0.399247 | 1.3:-6406422 | MS.gene051574:CDS |
| GTTATCACCATCAAGATGAT+TGG | 0.401723 | 1.3:+6406500 | None:intergenic |
| TTTATTCCAAACCAAATTCT+GGG | 0.404687 | 1.3:-6412313 | MS.gene051574:CDS |
| CTCACCACGAGCTATTGAAT+TGG | 0.405150 | 1.3:-6409825 | MS.gene051574:CDS |
| GTTTGCTGGTCTCAGGCGTT+GGG | 0.406051 | 1.3:-6406580 | MS.gene051574:intron |
| GGAGTCGGGGCTGACATGTT+TGG | 0.408447 | 1.3:-6406408 | MS.gene051574:CDS |
| TTTCAAAGACCGGCATTACT+TGG | 0.412980 | 1.3:-6410555 | MS.gene051574:CDS |
| ACGAGCTATTGAATTGGTTA+AGG | 0.421963 | 1.3:-6409819 | MS.gene051574:intron |
| TGAGACCCCAGAATTTGGTT+TGG | 0.422537 | 1.3:+6412307 | None:intergenic |
| CGCAGCAGCACATATCCATT+TGG | 0.435282 | 1.3:+6408704 | None:intergenic |
| CATGCATAAACAAAAGCATC+TGG | 0.435659 | 1.3:+6409854 | None:intergenic |
| AAGAGAAGCTAAGAAATATT+GGG | 0.435875 | 1.3:-6412167 | MS.gene051574:CDS |
| CAACATACCTGCAAATCAAC+TGG | 0.438434 | 1.3:-6405634 | MS.gene051574:CDS |
| TTGGAGTTAGGGTGTGGCAG+TGG | 0.441103 | 1.3:-6405532 | MS.gene051574:CDS |
| GCCGAGGCCAATCATCTTGA+TGG | 0.451444 | 1.3:-6406507 | MS.gene051574:CDS |
| TTTCTCCATTTACAGCCAAA+TGG | 0.454611 | 1.3:-6408719 | MS.gene051574:intron |
| GCATTACTTGGATAAAGAAT+GGG | 0.455655 | 1.3:-6410543 | MS.gene051574:CDS |
| GGCATTACTTGGATAAAGAA+TGG | 0.457337 | 1.3:-6410544 | MS.gene051574:CDS |
| ATTATGGAGATCAACCTTAG+AGG | 0.457883 | 1.3:-6405694 | MS.gene051574:CDS |
| TGGTTTGATGCTCTGGGAAT+CGG | 0.466752 | 1.3:-6405614 | MS.gene051574:CDS |
| AGGGTAGGTACCTCTCCAAA+AGG | 0.473644 | 1.3:+6412283 | None:intergenic |
| TGCAGTGTCCCCAGAAAAGA+TGG | 0.478351 | 1.3:-6408861 | MS.gene051574:CDS |
| TTTACTGAACAGGTTGGGTG+TGG | 0.479098 | 1.3:-6409914 | MS.gene051574:intron |
| CTGCTGCGAGACTATGCTAC+TGG | 0.479610 | 1.3:-6408689 | MS.gene051574:CDS |
| GGCTTCTTTACTGGAAGAGT+TGG | 0.494436 | 1.3:+6406527 | None:intergenic |
| TTCTGTTGATATTGTTACAA+TGG | 0.496744 | 1.3:-6409072 | MS.gene051574:intron |
| GAGTTAGGGTGTGGCAGTGG+TGG | 0.504655 | 1.3:-6405529 | MS.gene051574:CDS |
| TGGAGATCAACCTTAGAGGC+TGG | 0.507549 | 1.3:-6405690 | MS.gene051574:CDS |
| GAACAGGTTGGGTGTGGAGC+TGG | 0.509594 | 1.3:-6409908 | MS.gene051574:intron |
| CAGCGACAAAAGCGTGAACA+TGG | 0.510092 | 1.3:+6409135 | None:intergenic |
| ACCATCAAGATGATTGGCCT+CGG | 0.511832 | 1.3:+6406506 | None:intergenic |
| TTGAAACACAAAATACAGCT+TGG | 0.514474 | 1.3:+6406556 | None:intergenic |
| ATCAACTGGTTTGATGCTCT+GGG | 0.517488 | 1.3:-6405620 | MS.gene051574:CDS |
| AATCTCTTTGCATAAATCAT+CGG | 0.529631 | 1.3:+6409103 | None:intergenic |
| ATTTGCCACTTCAAACTCAA+AGG | 0.532302 | 1.3:-6412343 | MS.gene051574:CDS |
| TGCTACTGGAGATCTTGCTC+AGG | 0.535249 | 1.3:-6408675 | MS.gene051574:intron |
| TGCAACCTTTGAGTTTGAAG+TGG | 0.544850 | 1.3:+6412338 | None:intergenic |
| AAGAAGTTGAGAACCGCTCA+AGG | 0.548248 | 1.3:-6406707 | MS.gene051574:CDS |
| AGGGTATTGGAGTTAGGGTG+TGG | 0.549969 | 1.3:-6405538 | MS.gene051574:CDS |
| GTAACAATATCAACAGAAGA+TGG | 0.550718 | 1.3:+6409077 | None:intergenic |
| CAGTTGATTTGCAGGTATGT+TGG | 0.552753 | 1.3:+6405635 | None:intergenic |
| ATTCTTTATCCAAGTAATGC+CGG | 0.560199 | 1.3:+6410546 | None:intergenic |
| ATCTGTAGCAACTACTCGAT+CGG | 0.561693 | 1.3:+6405477 | None:intergenic |
| AAGGACAGAAGCCATTAATC+GGG | 0.567183 | 1.3:+6405591 | None:intergenic |
| CAAGGGAGTTGATAATGAAA+CGG | 0.570171 | 1.3:-6406689 | MS.gene051574:intron |
| TCTGGATATGATGCTATCAC+AGG | 0.575758 | 1.3:+6409872 | None:intergenic |
| ATTGACATGTCTGAGGGAGT+CGG | 0.576361 | 1.3:-6406423 | MS.gene051574:CDS |
| GGGTAGGTACCTCTCCAAAA+GGG | 0.580724 | 1.3:+6412284 | None:intergenic |
| ATCTCACGGCAATGGCATCA+GGG | 0.581718 | 1.3:-6412373 | None:intergenic |
| TCATTATCAACTCCCTTGAG+CGG | 0.584655 | 1.3:+6406694 | None:intergenic |
| GATCGAGTAGTTGCTACAGA+TGG | 0.598212 | 1.3:-6405475 | MS.gene051574:CDS |
| CTCTGTAATTGACATGTCTG+AGG | 0.603915 | 1.3:-6406430 | MS.gene051574:CDS |
| CTCTTCCAGTAAAGAAGCCG+AGG | 0.604507 | 1.3:-6406523 | MS.gene051574:CDS |
| TTATTCCAAACCAAATTCTG+GGG | 0.617390 | 1.3:-6412312 | MS.gene051574:CDS |
| TGACATGTCTGAGGGAGTCG+GGG | 0.622122 | 1.3:-6406421 | MS.gene051574:CDS |
| GTAGGAGAGATTCTCTGGCA+AGG | 0.625319 | 1.3:-6406948 | MS.gene051574:intron |
| TCTCACGGCAATGGCATCAG+GGG | 0.631546 | 1.3:-6412372 | None:intergenic |
| CTACAAACACCACAAAGACA+AGG | 0.634893 | 1.3:-6412137 | MS.gene051574:intron |
| CTTACGTCATACTCGTCGGA+AGG | 0.645016 | 1.3:+6406378 | None:intergenic |
| AGAAGTTGAGAACCGCTCAA+GGG | 0.645207 | 1.3:-6406706 | MS.gene051574:CDS |
| AGCGACAAAAGCGTGAACAT+GGG | 0.663872 | 1.3:+6409136 | None:intergenic |
| AAGCCTTACGTCATACTCGT+CGG | 0.678407 | 1.3:+6406374 | None:intergenic |
| CATTACTTGGATAAAGAATG+GGG | 0.679169 | 1.3:-6410542 | MS.gene051574:CDS |
| TCTGTAATTGACATGTCTGA+GGG | 0.683888 | 1.3:-6406429 | MS.gene051574:CDS |
| TTAACCAATTCAATAGCTCG+TGG | 0.686527 | 1.3:+6409821 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 6405432 | 6412384 | 6405432 | ID=MS.gene051574 |
| chr1.3 | mRNA | 6405432 | 6412384 | 6405432 | ID=MS.gene051574.t1;Parent=MS.gene051574 |
| chr1.3 | exon | 6412294 | 6412384 | 6412294 | ID=MS.gene051574.t1.exon1;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6412294 | 6412384 | 6412294 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6412138 | 6412199 | 6412138 | ID=MS.gene051574.t1.exon2;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6412138 | 6412199 | 6412138 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6410529 | 6410579 | 6410529 | ID=MS.gene051574.t1.exon3;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6410529 | 6410579 | 6410529 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6410404 | 6410430 | 6410404 | ID=MS.gene051574.t1.exon4;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6410404 | 6410430 | 6410404 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6409820 | 6409924 | 6409820 | ID=MS.gene051574.t1.exon5;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6409820 | 6409924 | 6409820 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6409073 | 6409180 | 6409073 | ID=MS.gene051574.t1.exon6;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6409073 | 6409180 | 6409073 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6408826 | 6408897 | 6408826 | ID=MS.gene051574.t1.exon7;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6408826 | 6408897 | 6408826 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6408676 | 6408726 | 6408676 | ID=MS.gene051574.t1.exon8;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6408676 | 6408726 | 6408676 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6406904 | 6406966 | 6406904 | ID=MS.gene051574.t1.exon9;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6406904 | 6406966 | 6406904 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6406690 | 6406814 | 6406690 | ID=MS.gene051574.t1.exon10;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6406690 | 6406814 | 6406690 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6406383 | 6406587 | 6406383 | ID=MS.gene051574.t1.exon11;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6406383 | 6406587 | 6406383 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| chr1.3 | exon | 6405432 | 6405716 | 6405432 | ID=MS.gene051574.t1.exon12;Parent=MS.gene051574.t1 |
| chr1.3 | CDS | 6405432 | 6405716 | 6405432 | ID=cds.MS.gene051574.t1;Parent=MS.gene051574.t1 |
| Gene Sequence |
| Protein sequence |
