Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46904.t1 | XP_024635998.1 | 96.2 | 52 | 2 | 0 | 38 | 89 | 137 | 188 | 1.40E-13 | 85.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46904.t1 | G7JBR3 | 96.2 | 52 | 2 | 0 | 38 | 89 | 137 | 188 | 9.8e-14 | 85.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049801 | MS.gene46904 | 0.810036 | 1.41E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene46904 | 0.804544 | 2.05E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene46904 | 0.816836 | 4.50E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene46904 | 0.818235 | 2.18E-52 | -1.69E-46 |
| MS.gene054823 | MS.gene46904 | 0.809236 | 2.09E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene46904 | 0.812147 | 4.91E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene46904 | 0.811902 | 5.55E-51 | -1.69E-46 |
| MS.gene058595 | MS.gene46904 | 0.81549 | 9.00E-52 | -1.69E-46 |
| MS.gene06010 | MS.gene46904 | 0.824653 | 7.18E-54 | -1.69E-46 |
| MS.gene060296 | MS.gene46904 | 0.831644 | 1.48E-55 | -1.69E-46 |
| MS.gene060802 | MS.gene46904 | 0.811673 | 6.22E-51 | -1.69E-46 |
| MS.gene060870 | MS.gene46904 | 0.82513 | 5.54E-54 | -1.69E-46 |
| MS.gene060998 | MS.gene46904 | 0.80548 | 1.31E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46904.t1 | MTR_3g108100 | 97.753 | 89 | 2 | 0 | 1 | 89 | 100 | 188 | 1.47e-53 | 169 |
| MS.gene46904.t1 | MTR_3g108090 | 85.294 | 68 | 10 | 0 | 22 | 89 | 122 | 189 | 5.69e-17 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACAGCATGCTCTGGTTC+TGG | 0.268354 | 3.1:+86145233 | None:intergenic |
| AGTTGTCGCTCTGCCTCCTT+TGG | 0.375124 | 3.1:+86145167 | None:intergenic |
| AGAGCGACAACTCTCCAAAA+AGG | 0.404514 | 3.1:-86145156 | MS.gene46904:CDS |
| TCAGTCTTCACAGCATGCTC+TGG | 0.423791 | 3.1:+86145227 | None:intergenic |
| TCTGCCTCCTTTGGCACCAC+AGG | 0.481597 | 3.1:+86145176 | None:intergenic |
| TCAGCGTGCTTCTTAACCTC+AGG | 0.486662 | 3.1:+86145203 | None:intergenic |
| AGATGACGGTGATGAAGAGG+AGG | 0.506605 | 3.1:-86145273 | MS.gene46904:CDS |
| ACTCAGAAAGAAAGTAATGA+TGG | 0.515814 | 3.1:-86145077 | MS.gene46904:CDS |
| TTTAGATGACGGTGATGAAG+AGG | 0.551847 | 3.1:-86145276 | MS.gene46904:CDS |
| AAAGGAACTTGAAGAGCTTG+AGG | 0.570478 | 3.1:-86145123 | MS.gene46904:CDS |
| TGAAGTTCCTGTGGTGCCAA+AGG | 0.576188 | 3.1:-86145183 | MS.gene46904:CDS |
| TTAACAGGATACTGAGAGCG+AGG | 0.605757 | 3.1:-86145306 | None:intergenic |
| AGTTCCTGTGGTGCCAAAGG+AGG | 0.616505 | 3.1:-86145180 | MS.gene46904:CDS |
| GAAGCACGCTGAAGTTCCTG+TGG | 0.664946 | 3.1:-86145192 | MS.gene46904:CDS |
| CAAGCTCAAGATGAGTCGCA+AGG | 0.671870 | 3.1:-86145053 | None:intergenic |
| TGCTGTGAAGACTGAACCTG+AGG | 0.675373 | 3.1:-86145219 | MS.gene46904:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTCAGAAAGAAAGTAATGA+TGG | - | chr3.1:86145276-86145295 | MS.gene46904:CDS | 30.0% | |
| CAAAAAGGAAAGGAAGAAAA+AGG | - | chr3.1:86145212-86145231 | MS.gene46904:CDS | 30.0% | |
| ! | CTTTTTCTTCCTTTCCTTTT+TGG | + | chr3.1:86145214-86145233 | None:intergenic | 30.0% |
| !! | GAGGAAGATATTTTAGATGA+CGG | - | chr3.1:86145066-86145085 | MS.gene46904:CDS | 30.0% |
| AAAGGAACTTGAAGAGCTTG+AGG | - | chr3.1:86145230-86145249 | MS.gene46904:CDS | 40.0% | |
| GACAACTCTCCAAAAAGGAA+AGG | - | chr3.1:86145202-86145221 | MS.gene46904:CDS | 40.0% | |
| ! | TTTAGATGACGGTGATGAAG+AGG | - | chr3.1:86145077-86145096 | MS.gene46904:CDS | 40.0% |
| !! | GAGGCTCTTTTAGCTGATTT+TGG | - | chr3.1:86145249-86145268 | MS.gene46904:CDS | 40.0% |
| AGAGCGACAACTCTCCAAAA+AGG | - | chr3.1:86145197-86145216 | MS.gene46904:CDS | 45.0% | |
| CAAGCTCAAGATGAGTCGCA+AGG | - | chr3.1:86145300-86145319 | MS.gene46904:CDS | 50.0% | |
| TCAGCGTGCTTCTTAACCTC+AGG | + | chr3.1:86145153-86145172 | None:intergenic | 50.0% | |
| TCAGTCTTCACAGCATGCTC+TGG | + | chr3.1:86145129-86145148 | None:intergenic | 50.0% | |
| TGCTGTGAAGACTGAACCTG+AGG | - | chr3.1:86145134-86145153 | MS.gene46904:CDS | 50.0% | |
| ! | AGATGACGGTGATGAAGAGG+AGG | - | chr3.1:86145080-86145099 | MS.gene46904:CDS | 50.0% |
| ! | TGAAGTTCCTGTGGTGCCAA+AGG | - | chr3.1:86145170-86145189 | MS.gene46904:CDS | 50.0% |
| ! | TTCACAGCATGCTCTGGTTC+TGG | + | chr3.1:86145123-86145142 | None:intergenic | 50.0% |
| AGTTGTCGCTCTGCCTCCTT+TGG | + | chr3.1:86145189-86145208 | None:intergenic | 55.0% | |
| GAAGCACGCTGAAGTTCCTG+TGG | - | chr3.1:86145161-86145180 | MS.gene46904:CDS | 55.0% | |
| ! | AGTTCCTGTGGTGCCAAAGG+AGG | - | chr3.1:86145173-86145192 | MS.gene46904:CDS | 55.0% |
| TCTGCCTCCTTTGGCACCAC+AGG | + | chr3.1:86145180-86145199 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 86145054 | 86145321 | 86145054 | ID=MS.gene46904 |
| chr3.1 | mRNA | 86145054 | 86145321 | 86145054 | ID=MS.gene46904.t1;Parent=MS.gene46904 |
| chr3.1 | exon | 86145054 | 86145321 | 86145054 | ID=MS.gene46904.t1.exon1;Parent=MS.gene46904.t1 |
| chr3.1 | CDS | 86145054 | 86145321 | 86145054 | ID=cds.MS.gene46904.t1;Parent=MS.gene46904.t1 |
| Gene Sequence |
| Protein sequence |
