Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46912.t1 | XP_003609200.2 | 98.9 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 1.40E-42 | 181.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46912.t1 | O81016 | 66.3 | 92 | 31 | 0 | 1 | 92 | 1 | 92 | 2.7e-29 | 129.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46912.t1 | G7JU55 | 98.9 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 1.0e-42 | 181.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050586 | MS.gene46912 | 0.812085 | 5.06E-51 | -1.69E-46 |
| MS.gene050762 | MS.gene46912 | 0.810085 | 1.37E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene46912 | 0.80936 | 1.97E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene46912 | 0.808064 | 3.72E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46912.t1 | MTR_4g113070 | 98.913 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 7.35e-60 | 196 |
| MS.gene46912.t1 | MTR_7g098740 | 43.956 | 91 | 50 | 1 | 1 | 91 | 14 | 103 | 3.40e-21 | 86.7 |
| MS.gene46912.t1 | MTR_1g011650 | 46.739 | 92 | 44 | 3 | 1 | 91 | 20 | 107 | 4.42e-21 | 86.3 |
| MS.gene46912.t1 | MTR_7g098750 | 42.857 | 91 | 51 | 1 | 1 | 91 | 14 | 103 | 6.58e-21 | 85.9 |
| MS.gene46912.t1 | MTR_7g098750 | 42.857 | 91 | 51 | 1 | 1 | 91 | 14 | 103 | 9.36e-21 | 85.1 |
| MS.gene46912.t1 | MTR_4g011630 | 44.944 | 89 | 48 | 1 | 3 | 91 | 27 | 114 | 1.33e-20 | 84.7 |
| MS.gene46912.t1 | MTR_7g098300 | 43.956 | 91 | 50 | 1 | 1 | 91 | 14 | 103 | 1.80e-20 | 84.3 |
| MS.gene46912.t1 | MTR_7g098370 | 40.659 | 91 | 53 | 1 | 1 | 91 | 14 | 103 | 1.94e-20 | 84.3 |
| MS.gene46912.t1 | MTR_7g098320 | 40.659 | 91 | 53 | 1 | 1 | 91 | 10 | 99 | 7.36e-20 | 82.8 |
| MS.gene46912.t1 | MTR_7g098320 | 40.659 | 91 | 53 | 1 | 1 | 91 | 10 | 99 | 9.73e-20 | 82.4 |
| MS.gene46912.t1 | MTR_2g102660 | 50.000 | 80 | 39 | 1 | 12 | 91 | 30 | 108 | 2.46e-19 | 81.3 |
| MS.gene46912.t1 | MTR_4g011640 | 45.556 | 90 | 46 | 2 | 3 | 91 | 25 | 112 | 3.36e-19 | 80.9 |
| MS.gene46912.t1 | MTR_7g098800 | 48.000 | 75 | 39 | 0 | 17 | 91 | 2 | 76 | 1.64e-18 | 79.0 |
| MS.gene46912.t1 | MTR_7g098780 | 42.697 | 89 | 50 | 1 | 1 | 89 | 29 | 116 | 6.17e-18 | 77.4 |
| MS.gene46912.t1 | MTR_2g102670 | 47.500 | 80 | 41 | 1 | 12 | 91 | 30 | 108 | 9.93e-18 | 76.6 |
| MS.gene46912.t1 | MTR_2g102640 | 47.500 | 80 | 41 | 1 | 12 | 91 | 30 | 108 | 1.73e-17 | 75.9 |
| MS.gene46912.t1 | MTR_7g098790 | 44.706 | 85 | 46 | 1 | 6 | 90 | 33 | 116 | 7.04e-17 | 73.6 |
| MS.gene46912.t1 | MTR_7g098760 | 45.000 | 80 | 43 | 1 | 12 | 91 | 25 | 103 | 1.04e-16 | 73.6 |
| MS.gene46912.t1 | MTR_4g011620 | 40.741 | 81 | 47 | 1 | 12 | 91 | 22 | 102 | 4.98e-16 | 71.6 |
| MS.gene46912.t1 | MTR_1g050525 | 39.773 | 88 | 45 | 2 | 12 | 91 | 30 | 117 | 6.36e-16 | 71.6 |
| MS.gene46912.t1 | MTR_7g104110 | 36.364 | 99 | 53 | 4 | 1 | 91 | 23 | 119 | 7.05e-15 | 68.6 |
| MS.gene46912.t1 | MTR_7g104150 | 36.000 | 100 | 53 | 4 | 1 | 91 | 28 | 125 | 2.20e-14 | 67.0 |
| MS.gene46912.t1 | MTR_3g107870 | 36.957 | 92 | 55 | 2 | 1 | 91 | 18 | 107 | 2.95e-14 | 66.6 |
| MS.gene46912.t1 | MTR_8g014360 | 41.463 | 82 | 38 | 2 | 20 | 91 | 38 | 119 | 4.39e-14 | 66.2 |
| MS.gene46912.t1 | MTR_8g014360 | 41.463 | 82 | 38 | 2 | 20 | 91 | 38 | 119 | 5.62e-14 | 65.9 |
| MS.gene46912.t1 | MTR_2g101090 | 34.000 | 100 | 52 | 3 | 6 | 91 | 21 | 120 | 1.05e-13 | 65.1 |
| MS.gene46912.t1 | MTR_7g104100 | 36.000 | 100 | 54 | 3 | 2 | 91 | 24 | 123 | 1.67e-13 | 64.7 |
| MS.gene46912.t1 | MTR_7g104130 | 36.559 | 93 | 52 | 3 | 4 | 91 | 35 | 125 | 6.29e-13 | 63.2 |
| MS.gene46912.t1 | MTR_7g104130 | 36.559 | 93 | 52 | 3 | 4 | 91 | 35 | 125 | 6.94e-13 | 62.8 |
| MS.gene46912.t1 | MTR_1g492950 | 31.111 | 90 | 57 | 2 | 4 | 89 | 40 | 128 | 1.30e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46912.t1 | AT2G26910 | 66.304 | 92 | 31 | 0 | 1 | 92 | 1 | 92 | 5.93e-40 | 140 |
| MS.gene46912.t1 | AT2G36380 | 44.444 | 90 | 43 | 4 | 6 | 91 | 43 | 129 | 3.38e-17 | 75.1 |
| MS.gene46912.t1 | AT1G15520 | 43.956 | 91 | 46 | 3 | 3 | 91 | 23 | 110 | 9.58e-17 | 73.9 |
| MS.gene46912.t1 | AT1G15520 | 43.956 | 91 | 46 | 3 | 3 | 91 | 23 | 110 | 3.28e-15 | 69.7 |
| MS.gene46912.t1 | AT1G66950 | 38.889 | 90 | 48 | 3 | 6 | 91 | 45 | 131 | 1.29e-14 | 67.8 |
| MS.gene46912.t1 | AT1G59870 | 34.737 | 95 | 53 | 2 | 6 | 91 | 33 | 127 | 6.12e-14 | 65.9 |
| MS.gene46912.t1 | AT1G15210 | 35.227 | 88 | 49 | 1 | 12 | 91 | 38 | 125 | 2.39e-13 | 64.3 |
| MS.gene46912.t1 | AT4G15230 | 39.474 | 76 | 42 | 2 | 20 | 91 | 26 | 101 | 1.54e-12 | 62.0 |
| MS.gene46912.t1 | AT3G16340 | 33.708 | 89 | 56 | 1 | 3 | 91 | 18 | 103 | 1.68e-12 | 62.0 |
| MS.gene46912.t1 | AT3G16340 | 33.708 | 89 | 56 | 1 | 3 | 91 | 18 | 103 | 1.68e-12 | 62.0 |
| MS.gene46912.t1 | AT4G15230 | 39.474 | 76 | 42 | 2 | 20 | 91 | 26 | 101 | 1.75e-12 | 61.6 |
| MS.gene46912.t1 | AT4G15230 | 39.474 | 76 | 42 | 2 | 20 | 91 | 26 | 101 | 1.75e-12 | 61.6 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 25 | 98 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 22 | 95 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT4G15215 | 39.189 | 74 | 43 | 1 | 20 | 91 | 25 | 98 | 3.77e-12 | 60.8 |
| MS.gene46912.t1 | AT2G37280 | 36.842 | 76 | 44 | 1 | 20 | 91 | 20 | 95 | 7.84e-12 | 60.1 |
| MS.gene46912.t1 | AT2G37280 | 36.842 | 76 | 44 | 1 | 20 | 91 | 20 | 95 | 7.90e-12 | 60.1 |
| MS.gene46912.t1 | AT2G37280 | 36.842 | 76 | 44 | 1 | 20 | 91 | 20 | 95 | 8.07e-12 | 60.1 |
| MS.gene46912.t1 | AT3G30842 | 36.842 | 95 | 54 | 3 | 3 | 91 | 8 | 102 | 6.20e-11 | 57.4 |
Find 20 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTATCACCGACGAGATTC+TGG | 0.280794 | 8.1:+13767422 | None:intergenic |
| TCGGAGTCATTTAGAGAATC+CGG | 0.397312 | 8.1:-13767520 | MS.gene46912:CDS |
| AGCAGAAACTTATTCTAGAA+AGG | 0.414979 | 8.1:-13767366 | MS.gene46912:CDS |
| GGTATAATTACGCGTCGAAT+CGG | 0.417052 | 8.1:+13767290 | None:intergenic |
| TAAGTTTCTGCTCTTGCGCT+TGG | 0.440485 | 8.1:+13767377 | None:intergenic |
| CTTCTCGCTCGTTTGTAAGT+TGG | 0.463318 | 8.1:+13767454 | None:intergenic |
| CGTCGAATCGGCTTCGCATA+CGG | 0.472524 | 8.1:+13767302 | None:intergenic |
| TAAAGCTTCTTCGTCTTCCC+CGG | 0.493259 | 8.1:+13767501 | None:intergenic |
| TTCTCTAAATGACTCCGATC+TGG | 0.497544 | 8.1:+13767525 | None:intergenic |
| CGGAGTCATTTAGAGAATCC+GGG | 0.503400 | 8.1:-13767519 | MS.gene46912:CDS |
| ATACGGTGGAAGAATCTCTC+AGG | 0.552092 | 8.1:+13767319 | None:intergenic |
| ACTTACAAACGAGCGAGAAG+AGG | 0.577823 | 8.1:-13767451 | MS.gene46912:CDS |
| AAATGACTCCGATCTGGCGA+AGG | 0.580474 | 8.1:+13767531 | None:intergenic |
| GGAAAATGCCTTCGCCAGAT+CGG | 0.591370 | 8.1:-13767539 | MS.gene46912:CDS |
| AAGACGAAGAAGCTTTACGC+TGG | 0.596919 | 8.1:-13767495 | MS.gene46912:CDS |
| AGACGAAGAAGCTTTACGCT+GGG | 0.627774 | 8.1:-13767494 | MS.gene46912:CDS |
| TTTCGCAATGTGGAACTCGG+CGG | 0.650830 | 8.1:-13767560 | None:intergenic |
| GGAATCTTCCAGAATCTCGT+CGG | 0.661869 | 8.1:-13767430 | MS.gene46912:CDS |
| CGAATCGGCTTCGCATACGG+TGG | 0.665694 | 8.1:+13767305 | None:intergenic |
| GGAGTCATTTAGAGAATCCG+GGG | 0.673499 | 8.1:-13767518 | MS.gene46912:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGCAGAAACTTATTCTAGAA+AGG | - | chr8.1:13767484-13767503 | MS.gene46912:CDS | 30.0% |
| TCGGAGTCATTTAGAGAATC+CGG | - | chr8.1:13767330-13767349 | MS.gene46912:CDS | 40.0% | |
| TTCTCTAAATGACTCCGATC+TGG | + | chr8.1:13767328-13767347 | None:intergenic | 40.0% | |
| AAGACGAAGAAGCTTTACGC+TGG | - | chr8.1:13767355-13767374 | MS.gene46912:CDS | 45.0% | |
| ACTTACAAACGAGCGAGAAG+AGG | - | chr8.1:13767399-13767418 | MS.gene46912:CDS | 45.0% | |
| AGACGAAGAAGCTTTACGCT+GGG | - | chr8.1:13767356-13767375 | MS.gene46912:CDS | 45.0% | |
| ATACGGTGGAAGAATCTCTC+AGG | + | chr8.1:13767534-13767553 | None:intergenic | 45.0% | |
| CGGAGTCATTTAGAGAATCC+GGG | - | chr8.1:13767331-13767350 | MS.gene46912:CDS | 45.0% | |
| CTTCTCGCTCGTTTGTAAGT+TGG | + | chr8.1:13767399-13767418 | None:intergenic | 45.0% | |
| GGAATCTTCCAGAATCTCGT+CGG | - | chr8.1:13767420-13767439 | MS.gene46912:CDS | 45.0% | |
| GGAGTCATTTAGAGAATCCG+GGG | - | chr8.1:13767332-13767351 | MS.gene46912:CDS | 45.0% | |
| TAAAGCTTCTTCGTCTTCCC+CGG | + | chr8.1:13767352-13767371 | None:intergenic | 45.0% | |
| TAAGTTTCTGCTCTTGCGCT+TGG | + | chr8.1:13767476-13767495 | None:intergenic | 45.0% | |
| TCTTATCACCGACGAGATTC+TGG | + | chr8.1:13767431-13767450 | None:intergenic | 45.0% | |
| AAATGACTCCGATCTGGCGA+AGG | + | chr8.1:13767322-13767341 | None:intergenic | 50.0% | |
| GGAAAATGCCTTCGCCAGAT+CGG | - | chr8.1:13767311-13767330 | MS.gene46912:CDS | 50.0% | |
| ! | CGTCGAATCGGCTTCGCATA+CGG | + | chr8.1:13767551-13767570 | None:intergenic | 55.0% |
| ! | CGAATCGGCTTCGCATACGG+TGG | + | chr8.1:13767548-13767567 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 13767297 | 13767575 | 13767297 | ID=MS.gene46912 |
| chr8.1 | mRNA | 13767297 | 13767575 | 13767297 | ID=MS.gene46912.t1;Parent=MS.gene46912 |
| chr8.1 | exon | 13767297 | 13767575 | 13767297 | ID=MS.gene46912.t1.exon1;Parent=MS.gene46912.t1 |
| chr8.1 | CDS | 13767297 | 13767575 | 13767297 | ID=cds.MS.gene46912.t1;Parent=MS.gene46912.t1 |
| Gene Sequence |
| Protein sequence |
