Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47374.t1 | XP_003590402.1 | 98 | 153 | 3 | 0 | 1 | 153 | 394 | 546 | 1.70E-80 | 308.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47374.t1 | P78362 | 55.8 | 86 | 38 | 0 | 1 | 86 | 602 | 687 | 7.3e-24 | 111.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47374.t1 | G7I9C7 | 98.0 | 153 | 3 | 0 | 1 | 153 | 394 | 546 | 1.2e-80 | 308.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050556 | MS.gene47374 | -0.818066 | 2.38E-52 | -1.69E-46 |
| MS.gene052673 | MS.gene47374 | 0.800275 | 1.56E-48 | -1.69E-46 |
| MS.gene058289 | MS.gene47374 | -0.81006 | 1.39E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene47374 | -0.80188 | 7.31E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47374.t1 | MTR_1g061690 | 98.039 | 153 | 3 | 0 | 1 | 153 | 394 | 546 | 3.05e-108 | 318 |
| MS.gene47374.t1 | MTR_2g084555 | 67.123 | 146 | 46 | 1 | 1 | 146 | 393 | 536 | 5.35e-62 | 199 |
| MS.gene47374.t1 | MTR_4g056390 | 62.937 | 143 | 52 | 1 | 1 | 142 | 394 | 536 | 2.21e-57 | 187 |
| MS.gene47374.t1 | MTR_8g073250 | 65.882 | 85 | 29 | 0 | 1 | 85 | 326 | 410 | 2.58e-36 | 130 |
| MS.gene47374.t1 | MTR_5g024270 | 60.920 | 87 | 34 | 0 | 1 | 87 | 330 | 416 | 2.19e-33 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47374.t1 | AT3G44850 | 75.188 | 133 | 33 | 0 | 1 | 133 | 397 | 529 | 9.52e-68 | 214 |
| MS.gene47374.t1 | AT3G53030 | 65.541 | 148 | 46 | 2 | 1 | 147 | 382 | 525 | 5.03e-65 | 207 |
| MS.gene47374.t1 | AT5G22840 | 69.173 | 133 | 41 | 0 | 1 | 133 | 386 | 518 | 5.71e-60 | 194 |
| MS.gene47374.t1 | AT4G35500 | 62.069 | 87 | 33 | 0 | 1 | 87 | 332 | 418 | 1.81e-35 | 127 |
| MS.gene47374.t1 | AT2G17530 | 60.920 | 87 | 34 | 0 | 1 | 87 | 331 | 417 | 2.06e-35 | 127 |
| MS.gene47374.t1 | AT2G17530 | 60.920 | 87 | 34 | 0 | 1 | 87 | 331 | 417 | 2.06e-35 | 127 |
| MS.gene47374.t1 | AT4G35500 | 62.069 | 87 | 33 | 0 | 1 | 87 | 333 | 419 | 3.24e-35 | 127 |
Find 51 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCATAAGCACTTTATTTA+AGG | 0.062791 | 1.4:-50876790 | None:intergenic |
| CATCTTTGTCCTTGTTCTTC+TGG | 0.137125 | 1.4:-50877007 | None:intergenic |
| CGGGATTTCTTCAATAGATA+TGG | 0.256557 | 1.4:+50876732 | MS.gene47374:CDS |
| GCCCTTAAATAAAGTGCTTA+TGG | 0.273324 | 1.4:+50876788 | MS.gene47374:CDS |
| GAATGTAGGTCCCCGTCTTT+TGG | 0.274592 | 1.4:+50876929 | MS.gene47374:CDS |
| GCATTAATGATGGAACTTCT+TGG | 0.282888 | 1.4:+50876588 | None:intergenic |
| CCTTGGAATACTTTCTTTGA+TGG | 0.309821 | 1.4:-50877101 | None:intergenic |
| TCCATAAGCACTTTATTTAA+GGG | 0.317347 | 1.4:-50876789 | None:intergenic |
| ATATCCGTCGGCTGCGATTC+TGG | 0.344849 | 1.4:+50876766 | MS.gene47374:CDS |
| GAGGCCATGGAGGCTGGAAT+TGG | 0.365219 | 1.4:+50877035 | MS.gene47374:CDS |
| GAGAGGGAGGCCATGGAGGC+TGG | 0.404591 | 1.4:+50877029 | MS.gene47374:CDS |
| TGATTAGAAGACGGCATTGA+TGG | 0.409832 | 1.4:-50876954 | None:intergenic |
| TGAACTACTTGCGGCTGCCT+TGG | 0.440976 | 1.4:-50877118 | None:intergenic |
| CCGGCTGTAGGTCGTTTCTC+AGG | 0.474020 | 1.4:-50876882 | None:intergenic |
| GAAGTAGCAGTTTCAGCACC+AGG | 0.474460 | 1.4:-50876981 | None:intergenic |
| CATATCTATTGAAGAAATCC+CGG | 0.482137 | 1.4:-50876731 | None:intergenic |
| CAAAGATGAGAGGGAGGCCA+TGG | 0.484725 | 1.4:+50877022 | MS.gene47374:CDS |
| CATTGATGGCTCCAAAAGAC+GGG | 0.487038 | 1.4:-50876940 | None:intergenic |
| CGGATGTAGCAGGCATTGAC+CGG | 0.500642 | 1.4:-50876901 | None:intergenic |
| TATAATTCTCAGATCGCACT+AGG | 0.509453 | 1.4:+50876696 | MS.gene47374:intron |
| CATTCATCCACGGATGTAGC+AGG | 0.517627 | 1.4:-50876911 | None:intergenic |
| GTTTCCAATTCCAGCCTCCA+TGG | 0.523041 | 1.4:-50877039 | None:intergenic |
| GTCAATGCCTGCTACATCCG+TGG | 0.547156 | 1.4:+50876904 | MS.gene47374:CDS |
| GAACAAGGACAAAGATGAGA+GGG | 0.551701 | 1.4:+50877013 | MS.gene47374:CDS |
| CTACATCCGTGGATGAATGT+AGG | 0.555309 | 1.4:+50876915 | MS.gene47374:CDS |
| GCATTGATGGCTCCAAAAGA+CGG | 0.555544 | 1.4:-50876941 | None:intergenic |
| ATATCTATTGAAGAAATCCC+GGG | 0.566218 | 1.4:-50876730 | None:intergenic |
| AAGAAATTTATTACCTTCCG+CGG | 0.571931 | 1.4:-50876618 | None:intergenic |
| CAAGGACAAAGATGAGAGGG+AGG | 0.580802 | 1.4:+50877016 | MS.gene47374:CDS |
| TCTTGGAATGATGCCGCGGA+AGG | 0.584206 | 1.4:+50876605 | MS.gene47374:CDS |
| CTATCAAGATGAACTACTTG+CGG | 0.587404 | 1.4:-50877127 | None:intergenic |
| CCTGAGAAACGACCTACAGC+CGG | 0.605154 | 1.4:+50876882 | MS.gene47374:CDS |
| ATAATTCTCAGATCGCACTA+GGG | 0.611148 | 1.4:+50876697 | MS.gene47374:intron |
| AAGTAGCAGTTTCAGCACCA+GGG | 0.617453 | 1.4:-50876980 | None:intergenic |
| TTTGATGGAGAATGCATGAG+TGG | 0.621786 | 1.4:-50877086 | None:intergenic |
| CCGTCTTCTAATCACAACCC+TGG | 0.626806 | 1.4:+50876963 | MS.gene47374:CDS |
| CCATCAAAGAAAGTATTCCA+AGG | 0.627091 | 1.4:+50877101 | MS.gene47374:CDS |
| TACTTCTGACCAGAAGAACA+AGG | 0.631653 | 1.4:+50876998 | MS.gene47374:CDS |
| AGGGCCAGAATCGCAGCCGA+CGG | 0.635004 | 1.4:-50876770 | None:intergenic |
| AGAACAAGGACAAAGATGAG+AGG | 0.640119 | 1.4:+50877012 | MS.gene47374:CDS |
| AGATGAGAGGGAGGCCATGG+AGG | 0.643015 | 1.4:+50877025 | MS.gene47374:CDS |
| AACTTCTTGGAATGATGCCG+CGG | 0.644175 | 1.4:+50876601 | MS.gene47374:CDS |
| CCAGGGTTGTGATTAGAAGA+CGG | 0.645495 | 1.4:-50876963 | None:intergenic |
| CGGGGACCTACATTCATCCA+CGG | 0.647503 | 1.4:-50876921 | None:intergenic |
| GTGATTTGAGACATATCCGT+CGG | 0.649441 | 1.4:+50876754 | MS.gene47374:CDS |
| GTTTCTCAGGAACAAAGTCA+AGG | 0.651456 | 1.4:-50876869 | None:intergenic |
| TAATTCTCAGATCGCACTAG+GGG | 0.655674 | 1.4:+50876698 | MS.gene47374:intron |
| ATTCTCAGATCGCACTAGGG+GGG | 0.670913 | 1.4:+50876700 | MS.gene47374:intron |
| AGCAGGCATTGACCGGCTGT+AGG | 0.674560 | 1.4:-50876894 | None:intergenic |
| AATTCTCAGATCGCACTAGG+GGG | 0.677507 | 1.4:+50876699 | MS.gene47374:intron |
| ATTGATGGCTCCAAAAGACG+GGG | 0.727950 | 1.4:-50876939 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGATCATAAATGAAAAGAAA+TGG | - | chr1.4:50876648-50876667 | None:intergenic | 20.0% |
| ! | CATAAATGAAAAGAAATGGA+TGG | - | chr1.4:50876644-50876663 | None:intergenic | 25.0% |
| !! | TCCATAAGCACTTTATTTAA+GGG | - | chr1.4:50876792-50876811 | None:intergenic | 25.0% |
| AAGAAATTTATTACCTTCCG+CGG | - | chr1.4:50876621-50876640 | None:intergenic | 30.0% | |
| ATATCTATTGAAGAAATCCC+GGG | - | chr1.4:50876733-50876752 | None:intergenic | 30.0% | |
| CATATCTATTGAAGAAATCC+CGG | - | chr1.4:50876734-50876753 | None:intergenic | 30.0% | |
| ! | CTCCATAAGCACTTTATTTA+AGG | - | chr1.4:50876793-50876812 | None:intergenic | 30.0% |
| ATAATTCTCAGATCGCACTA+GGG | + | chr1.4:50876697-50876716 | MS.gene47374:intron | 35.0% | |
| CCTTGGAATACTTTCTTTGA+TGG | - | chr1.4:50877104-50877123 | None:intergenic | 35.0% | |
| CGGGATTTCTTCAATAGATA+TGG | + | chr1.4:50876732-50876751 | MS.gene47374:CDS | 35.0% | |
| TATAATTCTCAGATCGCACT+AGG | + | chr1.4:50876696-50876715 | MS.gene47374:intron | 35.0% | |
| ! | AAGTGACATGAGTGACTTTT+TGG | + | chr1.4:50876839-50876858 | MS.gene47374:CDS | 35.0% |
| ! | GCCCTTAAATAAAGTGCTTA+TGG | + | chr1.4:50876788-50876807 | MS.gene47374:CDS | 35.0% |
| !! | CCATCAAAGAAAGTATTCCA+AGG | + | chr1.4:50877101-50877120 | MS.gene47374:CDS | 35.0% |
| AGAACAAGGACAAAGATGAG+AGG | + | chr1.4:50877012-50877031 | MS.gene47374:CDS | 40.0% | |
| CATCTTTGTCCTTGTTCTTC+TGG | - | chr1.4:50877010-50877029 | None:intergenic | 40.0% | |
| GAACAAGGACAAAGATGAGA+GGG | + | chr1.4:50877013-50877032 | MS.gene47374:CDS | 40.0% | |
| GTTTCTCAGGAACAAAGTCA+AGG | - | chr1.4:50876872-50876891 | None:intergenic | 40.0% | |
| TAATTCTCAGATCGCACTAG+GGG | + | chr1.4:50876698-50876717 | MS.gene47374:intron | 40.0% | |
| TACTTCTGACCAGAAGAACA+AGG | + | chr1.4:50876998-50877017 | MS.gene47374:CDS | 40.0% | |
| TTTGATGGAGAATGCATGAG+TGG | - | chr1.4:50877089-50877108 | None:intergenic | 40.0% | |
| ! | GTGATTTGAGACATATCCGT+CGG | + | chr1.4:50876754-50876773 | MS.gene47374:CDS | 40.0% |
| ! | GTTTGCAGTTAACTTGAGTC+TGG | + | chr1.4:50876671-50876690 | MS.gene47374:intron | 40.0% |
| ! | TGATTAGAAGACGGCATTGA+TGG | - | chr1.4:50876957-50876976 | None:intergenic | 40.0% |
| AACTTCTTGGAATGATGCCG+CGG | + | chr1.4:50876601-50876620 | MS.gene47374:CDS | 45.0% | |
| AAGTAGCAGTTTCAGCACCA+GGG | - | chr1.4:50876983-50877002 | None:intergenic | 45.0% | |
| AATTCTCAGATCGCACTAGG+GGG | + | chr1.4:50876699-50876718 | MS.gene47374:intron | 45.0% | |
| CCAGGGTTGTGATTAGAAGA+CGG | - | chr1.4:50876966-50876985 | None:intergenic | 45.0% | |
| CTACATCCGTGGATGAATGT+AGG | + | chr1.4:50876915-50876934 | MS.gene47374:CDS | 45.0% | |
| ! | ATTGATGGCTCCAAAAGACG+GGG | - | chr1.4:50876942-50876961 | None:intergenic | 45.0% |
| ! | CATTGATGGCTCCAAAAGAC+GGG | - | chr1.4:50876943-50876962 | None:intergenic | 45.0% |
| ! | GCATTGATGGCTCCAAAAGA+CGG | - | chr1.4:50876944-50876963 | None:intergenic | 45.0% |
| ATTCTCAGATCGCACTAGGG+GGG | + | chr1.4:50876700-50876719 | MS.gene47374:intron | 50.0% | |
| CAAGGACAAAGATGAGAGGG+AGG | + | chr1.4:50877016-50877035 | MS.gene47374:CDS | 50.0% | |
| CATTCATCCACGGATGTAGC+AGG | - | chr1.4:50876914-50876933 | None:intergenic | 50.0% | |
| CCGTCTTCTAATCACAACCC+TGG | + | chr1.4:50876963-50876982 | MS.gene47374:CDS | 50.0% | |
| GAAGTAGCAGTTTCAGCACC+AGG | - | chr1.4:50876984-50877003 | None:intergenic | 50.0% | |
| GTTTCCAATTCCAGCCTCCA+TGG | - | chr1.4:50877042-50877061 | None:intergenic | 50.0% | |
| ! | GAATGTAGGTCCCCGTCTTT+TGG | + | chr1.4:50876929-50876948 | MS.gene47374:CDS | 50.0% |
| CAAAGATGAGAGGGAGGCCA+TGG | + | chr1.4:50877022-50877041 | MS.gene47374:CDS | 55.0% | |
| CCTGAGAAACGACCTACAGC+CGG | + | chr1.4:50876882-50876901 | MS.gene47374:CDS | 55.0% | |
| CGGGGACCTACATTCATCCA+CGG | - | chr1.4:50876924-50876943 | None:intergenic | 55.0% | |
| GTCAATGCCTGCTACATCCG+TGG | + | chr1.4:50876904-50876923 | MS.gene47374:CDS | 55.0% | |
| TCTTGGAATGATGCCGCGGA+AGG | + | chr1.4:50876605-50876624 | MS.gene47374:CDS | 55.0% | |
| ! | ATATCCGTCGGCTGCGATTC+TGG | + | chr1.4:50876766-50876785 | MS.gene47374:CDS | 55.0% |
| !! | CGGATGTAGCAGGCATTGAC+CGG | - | chr1.4:50876904-50876923 | None:intergenic | 55.0% |
| AGATGAGAGGGAGGCCATGG+AGG | + | chr1.4:50877025-50877044 | MS.gene47374:CDS | 60.0% | |
| CCGGCTGTAGGTCGTTTCTC+AGG | - | chr1.4:50876885-50876904 | None:intergenic | 60.0% | |
| GAGGCCATGGAGGCTGGAAT+TGG | + | chr1.4:50877035-50877054 | MS.gene47374:CDS | 60.0% | |
| !! | ACTAGGGGGGCGTTATTCCC+GGG | + | chr1.4:50876713-50876732 | MS.gene47374:CDS | 60.0% |
| !! | AGCAGGCATTGACCGGCTGT+AGG | - | chr1.4:50876897-50876916 | None:intergenic | 60.0% |
| !! | CACTAGGGGGGCGTTATTCC+CGG | + | chr1.4:50876712-50876731 | MS.gene47374:CDS | 60.0% |
| AGGGCCAGAATCGCAGCCGA+CGG | - | chr1.4:50876773-50876792 | None:intergenic | 65.0% | |
| GAGAGGGAGGCCATGGAGGC+TGG | + | chr1.4:50877029-50877048 | MS.gene47374:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 50876594 | 50877136 | 50876594 | ID=MS.gene47374 |
| chr1.4 | mRNA | 50876594 | 50877136 | 50876594 | ID=MS.gene47374.t1;Parent=MS.gene47374 |
| chr1.4 | exon | 50876594 | 50876626 | 50876594 | ID=MS.gene47374.t1.exon1;Parent=MS.gene47374.t1 |
| chr1.4 | CDS | 50876594 | 50876626 | 50876594 | ID=cds.MS.gene47374.t1;Parent=MS.gene47374.t1 |
| chr1.4 | exon | 50876708 | 50877136 | 50876708 | ID=MS.gene47374.t1.exon2;Parent=MS.gene47374.t1 |
| chr1.4 | CDS | 50876708 | 50877136 | 50876708 | ID=cds.MS.gene47374.t1;Parent=MS.gene47374.t1 |
| Gene Sequence |
| Protein sequence |
