Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060380.t1 | XP_013450616.1 | 91.8 | 244 | 10 | 2 | 43 | 277 | 1 | 243 | 7.00E-109 | 403.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060380.t1 | Q9SSE9 | 40.4 | 109 | 60 | 2 | 173 | 277 | 154 | 261 | 1.2e-16 | 88.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060380.t1 | A0A072U4M1 | 91.8 | 244 | 10 | 2 | 43 | 277 | 1 | 243 | 5.1e-109 | 403.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050556 | MS.gene060380 | 0.804966 | 1.68E-49 | -1.69E-46 |
| MS.gene050657 | MS.gene060380 | 0.812228 | 4.71E-51 | -1.69E-46 |
| MS.gene051102 | MS.gene060380 | 0.80971 | 1.65E-50 | -1.69E-46 |
| MS.gene052609 | MS.gene060380 | 0.815991 | 6.96E-52 | -1.69E-46 |
| MS.gene055864 | MS.gene060380 | 0.821144 | 4.72E-53 | -1.69E-46 |
| MS.gene058289 | MS.gene060380 | 0.838334 | 3.06E-57 | -1.69E-46 |
| MS.gene058638 | MS.gene060380 | 0.806023 | 1.01E-49 | -1.69E-46 |
| MS.gene058639 | MS.gene060380 | 0.803397 | 3.55E-49 | -1.69E-46 |
| MS.gene058647 | MS.gene060380 | 0.805139 | 1.54E-49 | -1.69E-46 |
| MS.gene058758 | MS.gene060380 | 0.823741 | 1.18E-53 | -1.69E-46 |
| MS.gene059713 | MS.gene060380 | 0.801334 | 9.46E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene063132 | 0.806362 | 8.53E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene06324 | 0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene065593 | 0.847093 | 1.44E-59 | -1.69E-46 |
| MS.gene060380 | MS.gene065908 | 0.845759 | 3.32E-59 | -1.69E-46 |
| MS.gene060380 | MS.gene065934 | 0.830742 | 2.47E-55 | -1.69E-46 |
| MS.gene060380 | MS.gene066405 | 0.8353 | 1.82E-56 | -1.69E-46 |
| MS.gene060380 | MS.gene066896 | 0.81559 | 8.55E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene069410 | 0.800015 | 1.76E-48 | -1.69E-46 |
| MS.gene060380 | MS.gene069690 | 0.817888 | 2.61E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene071699 | 0.842562 | 2.40E-58 | -1.69E-46 |
| MS.gene060380 | MS.gene071779 | 0.805358 | 1.39E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene07279 | 0.831798 | 1.36E-55 | -1.69E-46 |
| MS.gene060380 | MS.gene073390 | 0.832194 | 1.09E-55 | -1.69E-46 |
| MS.gene060380 | MS.gene07382 | 0.815588 | 8.56E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene20621 | 0.802544 | 5.33E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene20718 | 0.813295 | 2.75E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene20801 | 0.809427 | 1.90E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene21826 | 0.824973 | 6.04E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene23963 | 0.808737 | 2.67E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene24203 | 0.841376 | 4.93E-58 | -1.69E-46 |
| MS.gene060380 | MS.gene24229 | 0.804961 | 1.68E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene24417 | 0.812457 | 4.20E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene24903 | 0.806986 | 6.30E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene25217 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene060380 | MS.gene25220 | 0.801694 | 7.98E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene25468 | 0.85473 | 1.01E-61 | -1.69E-46 |
| MS.gene060380 | MS.gene25469 | 0.820332 | 7.25E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene25471 | 0.8228 | 1.95E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene26396 | 0.821741 | 3.44E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene27006 | 0.824096 | 9.71E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene27026 | 0.819387 | 1.19E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene27269 | 0.813061 | 3.10E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene27288 | 0.814601 | 1.42E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene27401 | 0.804432 | 2.17E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene27700 | 0.816116 | 6.53E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene27919 | 0.80612 | 9.60E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene28536 | 0.827726 | 1.33E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene29041 | 0.823387 | 1.42E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene29361 | 0.842829 | 2.04E-58 | -1.69E-46 |
| MS.gene060380 | MS.gene32081 | 0.802793 | 4.74E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene34485 | 0.818589 | 1.81E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene34691 | 0.843951 | 1.02E-58 | -1.69E-46 |
| MS.gene060380 | MS.gene34937 | 0.810739 | 9.92E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene36567 | 0.800449 | 1.43E-48 | -1.69E-46 |
| MS.gene060380 | MS.gene37007 | 0.805469 | 1.31E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene38378 | 0.824964 | 6.07E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene40093 | 0.813473 | 2.51E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene41427 | 0.843086 | 1.74E-58 | -1.69E-46 |
| MS.gene060380 | MS.gene43202 | 0.843211 | 1.61E-58 | -1.69E-46 |
| MS.gene060380 | MS.gene44427 | 0.803625 | 3.19E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene44967 | 0.831128 | 1.99E-55 | -1.69E-46 |
| MS.gene060380 | MS.gene45624 | 0.822791 | 1.96E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene47374 | -0.80188 | 7.31E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene47554 | 0.805587 | 1.24E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene47581 | 0.811283 | 7.57E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene47695 | 0.832311 | 1.02E-55 | -1.69E-46 |
| MS.gene060380 | MS.gene47747 | 0.826524 | 2.59E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene48054 | 0.803142 | 4.01E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene48283 | 0.841322 | 5.10E-58 | -1.69E-46 |
| MS.gene060380 | MS.gene49467 | 0.85873 | 6.74E-63 | -1.69E-46 |
| MS.gene060380 | MS.gene50869 | 0.816262 | 6.06E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene51028 | 0.831115 | 2.00E-55 | -1.69E-46 |
| MS.gene060380 | MS.gene51029 | 0.844518 | 7.19E-59 | -1.69E-46 |
| MS.gene060380 | MS.gene52437 | 0.815799 | 7.68E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene52438 | 0.810099 | 1.36E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene52956 | 0.816282 | 5.99E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene54280 | 0.813513 | 2.46E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene54904 | 0.801025 | 1.09E-48 | -1.69E-46 |
| MS.gene060380 | MS.gene58134 | 0.812473 | 4.16E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene58508 | 0.817013 | 4.11E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene58617 | 0.858793 | 6.45E-63 | -1.69E-46 |
| MS.gene060380 | MS.gene59366 | 0.820297 | 7.39E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene59645 | 0.806234 | 9.08E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene60142 | 0.80992 | 1.49E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene60143 | 0.817411 | 3.34E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene60144 | 0.821696 | 3.52E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene60163 | 0.823377 | 1.43E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene63961 | 0.822423 | 2.39E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene65300 | 0.827706 | 1.35E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene67141 | 0.802128 | 6.50E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene67553 | 0.833987 | 3.89E-56 | -1.69E-46 |
| MS.gene060380 | MS.gene67841 | 0.817895 | 2.60E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene68360 | 0.801954 | 7.06E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene68783 | 0.81701 | 4.12E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene70331 | 0.802371 | 5.79E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene73245 | 0.811362 | 7.27E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene73332 | 0.812195 | 4.79E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene76317 | 0.808501 | 3.00E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene76506 | 0.823085 | 1.67E-53 | -1.69E-46 |
| MS.gene060380 | MS.gene78230 | 0.801543 | 8.57E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene82806 | 0.813075 | 3.08E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene82989 | 0.811513 | 6.74E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene83689 | 0.858714 | 6.81E-63 | -1.69E-46 |
| MS.gene060380 | MS.gene84498 | 0.832643 | 8.41E-56 | -1.69E-46 |
| MS.gene060380 | MS.gene84840 | 0.859276 | 4.63E-63 | -1.69E-46 |
| MS.gene060380 | MS.gene85122 | 0.829358 | 5.38E-55 | -1.69E-46 |
| MS.gene060380 | MS.gene86267 | 0.837676 | 4.52E-57 | -1.69E-46 |
| MS.gene060380 | MS.gene87358 | 0.812139 | 4.93E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene87622 | 0.806712 | 7.20E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene88254 | 0.826231 | 3.04E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene88450 | 0.819202 | 1.31E-52 | -1.69E-46 |
| MS.gene060380 | MS.gene88649 | 0.827291 | 1.70E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene89368 | 0.814126 | 1.81E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene90431 | 0.827002 | 1.99E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene90727 | 0.802396 | 5.72E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene90763 | 0.827385 | 1.61E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene91208 | 0.807366 | 5.24E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene92196 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene93037 | 0.802172 | 6.36E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene94546 | 0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene95562 | 0.82743 | 1.57E-54 | -1.69E-46 |
| MS.gene060380 | MS.gene96598 | 0.80517 | 1.52E-49 | -1.69E-46 |
| MS.gene060380 | MS.gene97459 | 0.810021 | 1.42E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene99525 | 0.823627 | 1.25E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060380.t1 | MTR_7g117445 | 92.213 | 244 | 9 | 2 | 43 | 277 | 1 | 243 | 1.46e-152 | 447 |
| MS.gene060380.t1 | MTR_7g117445 | 92.213 | 244 | 9 | 2 | 43 | 277 | 1 | 243 | 5.07e-152 | 448 |
| MS.gene060380.t1 | MTR_7g117445 | 92.213 | 244 | 9 | 2 | 43 | 277 | 1 | 243 | 7.61e-152 | 447 |
| MS.gene060380.t1 | MTR_7g117445 | 92.213 | 244 | 9 | 2 | 43 | 277 | 1 | 243 | 1.50e-151 | 447 |
| MS.gene060380.t1 | MTR_1g008060 | 51.200 | 125 | 59 | 1 | 154 | 276 | 850 | 974 | 6.46e-37 | 139 |
| MS.gene060380.t1 | MTR_1g008060 | 51.200 | 125 | 59 | 1 | 154 | 276 | 841 | 965 | 6.77e-37 | 139 |
| MS.gene060380.t1 | MTR_1g114130 | 48.214 | 112 | 54 | 1 | 170 | 277 | 323 | 434 | 1.85e-27 | 112 |
| MS.gene060380.t1 | MTR_1g114150 | 49.558 | 113 | 51 | 2 | 170 | 277 | 339 | 450 | 7.04e-27 | 110 |
| MS.gene060380.t1 | MTR_1g114150 | 49.558 | 113 | 51 | 2 | 170 | 277 | 339 | 450 | 9.55e-27 | 109 |
| MS.gene060380.t1 | MTR_1g114070 | 36.269 | 193 | 107 | 4 | 89 | 277 | 152 | 332 | 1.90e-26 | 108 |
| MS.gene060380.t1 | MTR_5g065200 | 35.519 | 183 | 93 | 4 | 100 | 277 | 86 | 248 | 1.64e-24 | 103 |
| MS.gene060380.t1 | MTR_1g083120 | 37.209 | 172 | 102 | 5 | 111 | 277 | 132 | 302 | 3.39e-23 | 99.4 |
| MS.gene060380.t1 | MTR_1g083120 | 44.167 | 120 | 62 | 3 | 161 | 277 | 202 | 319 | 3.73e-23 | 99.4 |
| MS.gene060380.t1 | MTR_1g083120 | 43.697 | 119 | 64 | 2 | 161 | 277 | 185 | 302 | 3.82e-23 | 99.4 |
| MS.gene060380.t1 | MTR_1g083120 | 37.209 | 172 | 102 | 5 | 111 | 277 | 132 | 302 | 4.06e-23 | 99.4 |
| MS.gene060380.t1 | MTR_4g132540 | 40.566 | 106 | 60 | 2 | 174 | 277 | 168 | 272 | 2.48e-19 | 88.2 |
| MS.gene060380.t1 | MTR_8g062940 | 39.815 | 108 | 61 | 2 | 174 | 277 | 33 | 140 | 6.65e-17 | 80.1 |
| MS.gene060380.t1 | MTR_2g024140 | 40.566 | 106 | 60 | 2 | 174 | 277 | 34 | 138 | 1.22e-16 | 80.1 |
| MS.gene060380.t1 | MTR_1g069565 | 38.961 | 154 | 79 | 5 | 131 | 277 | 106 | 251 | 2.89e-14 | 73.2 |
| MS.gene060380.t1 | MTR_1g069565 | 38.961 | 154 | 79 | 5 | 131 | 277 | 106 | 251 | 2.91e-14 | 73.2 |
| MS.gene060380.t1 | MTR_4g091520 | 38.596 | 114 | 59 | 4 | 174 | 277 | 260 | 372 | 3.91e-14 | 72.8 |
| MS.gene060380.t1 | MTR_4g091520 | 38.596 | 114 | 59 | 4 | 174 | 277 | 260 | 372 | 4.19e-14 | 72.8 |
| MS.gene060380.t1 | MTR_4g091520 | 38.596 | 114 | 59 | 4 | 174 | 277 | 260 | 372 | 4.25e-14 | 72.8 |
| MS.gene060380.t1 | MTR_4g091520 | 38.596 | 114 | 59 | 4 | 174 | 277 | 260 | 372 | 4.83e-14 | 72.4 |
| MS.gene060380.t1 | MTR_2g024270 | 38.462 | 91 | 53 | 2 | 174 | 262 | 154 | 243 | 3.56e-12 | 66.6 |
| MS.gene060380.t1 | MTR_2g011630 | 34.234 | 111 | 63 | 4 | 174 | 277 | 207 | 314 | 7.14e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060380.t1 | AT4G00990 | 48.113 | 106 | 51 | 3 | 174 | 276 | 80 | 184 | 4.08e-24 | 102 |
| MS.gene060380.t1 | AT4G00990 | 48.113 | 106 | 51 | 3 | 174 | 276 | 80 | 184 | 4.59e-24 | 102 |
| MS.gene060380.t1 | AT4G00990 | 48.113 | 106 | 51 | 3 | 174 | 276 | 80 | 184 | 4.76e-24 | 102 |
| MS.gene060380.t1 | AT1G09060 | 44.248 | 113 | 59 | 2 | 169 | 277 | 199 | 311 | 6.33e-22 | 95.9 |
| MS.gene060380.t1 | AT1G09060 | 44.248 | 113 | 59 | 2 | 169 | 277 | 199 | 311 | 6.33e-22 | 95.9 |
| MS.gene060380.t1 | AT1G09060 | 44.248 | 113 | 59 | 2 | 169 | 277 | 213 | 325 | 6.92e-22 | 95.5 |
| MS.gene060380.t1 | AT1G09060 | 44.248 | 113 | 59 | 2 | 169 | 277 | 213 | 325 | 6.92e-22 | 95.5 |
| MS.gene060380.t1 | AT1G11950 | 34.188 | 117 | 75 | 1 | 163 | 277 | 182 | 298 | 1.29e-18 | 85.9 |
| MS.gene060380.t1 | AT1G11950 | 34.188 | 117 | 75 | 1 | 163 | 277 | 182 | 298 | 1.35e-18 | 85.9 |
| MS.gene060380.t1 | AT1G11950 | 34.188 | 117 | 75 | 1 | 163 | 277 | 182 | 298 | 1.35e-18 | 85.9 |
| MS.gene060380.t1 | AT3G07610 | 40.541 | 111 | 57 | 3 | 173 | 277 | 154 | 261 | 4.09e-18 | 84.7 |
| MS.gene060380.t1 | AT3G07610 | 40.541 | 111 | 57 | 3 | 173 | 277 | 154 | 261 | 4.68e-18 | 84.3 |
| MS.gene060380.t1 | AT3G07610 | 41.284 | 109 | 59 | 2 | 173 | 277 | 154 | 261 | 1.19e-17 | 83.2 |
| MS.gene060380.t1 | AT1G62310 | 31.111 | 135 | 90 | 2 | 144 | 276 | 180 | 313 | 2.19e-16 | 79.3 |
Find 62 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAAACTTTCACCCTTCT+TGG | 0.166875 | 7.4:+1227515 | None:intergenic |
| TCATCACGTTACCCCTTATT+TGG | 0.218499 | 7.4:+1226965 | None:intergenic |
| TTGAAAGTGTGTTATCTTTC+CGG | 0.220977 | 7.4:+1228734 | None:intergenic |
| TAGGAGAAGGAAGGTGATTT+TGG | 0.233228 | 7.4:-1228273 | MS.gene060380:CDS |
| GCACGCTTCGCCACCGCTTT+AGG | 0.248072 | 7.4:+1228532 | None:intergenic |
| CTAGAGATTGAAACCAAATA+AGG | 0.300996 | 7.4:-1226978 | None:intergenic |
| TGAAAGTGTGTTATCTTTCC+GGG | 0.350478 | 7.4:+1228735 | None:intergenic |
| TTCTCTTCTCACCCGTAGTT+TGG | 0.380702 | 7.4:+1227795 | None:intergenic |
| CTAGTTCCAAGCTCTGTTCC+CGG | 0.395888 | 7.4:+1226997 | None:intergenic |
| TAGAGATTGAAACCAAATAA+GGG | 0.415090 | 7.4:-1226977 | None:intergenic |
| GGGACTAATGGATGTGTATA+AGG | 0.420271 | 7.4:-1228247 | MS.gene060380:CDS |
| TGACACTGGCATCTATTTCT+CGG | 0.421598 | 7.4:+1227078 | None:intergenic |
| TAACTGCAAAGCTTGCTTAC+GGG | 0.421761 | 7.4:-1227216 | MS.gene060380:CDS |
| CACGTTCTCAAATTCCTCTC+GGG | 0.423212 | 7.4:+1227262 | None:intergenic |
| GTTACGAATGCATTGAGAAC+TGG | 0.440992 | 7.4:-1227422 | MS.gene060380:intron |
| CATGTACTAACTTGATGAAT+CGG | 0.446841 | 7.4:+1228367 | None:intergenic |
| CGGTGGATATGCCTCAGAAC+TGG | 0.464151 | 7.4:+1227017 | None:intergenic |
| TTTCTTCTTCTTCTTTCTCG+TGG | 0.466094 | 7.4:+1228390 | None:intergenic |
| AATTGAAGATACCAAACAAC+TGG | 0.474075 | 7.4:-1228205 | MS.gene060380:intron |
| TATCCACCGGGAACAGAGCT+TGG | 0.485075 | 7.4:-1227003 | MS.gene060380:CDS |
| GTAACTGCAAAGCTTGCTTA+CGG | 0.492778 | 7.4:-1227217 | MS.gene060380:CDS |
| GCGAAGCGTGCCAACGTCGC+CGG | 0.497824 | 7.4:-1228520 | MS.gene060380:CDS |
| ACACGTTCTCAAATTCCTCT+CGG | 0.497938 | 7.4:+1227261 | None:intergenic |
| AAGATGCAGAGTCTAACAAT+AGG | 0.506744 | 7.4:-1228477 | MS.gene060380:CDS |
| GTGGTTCTCTCCGGCGACGT+TGG | 0.507803 | 7.4:+1228510 | None:intergenic |
| ACGGGAGTTTCCTGTGCTGA+TGG | 0.512922 | 7.4:-1227198 | MS.gene060380:intron |
| GGATATGCCTCAGAACTGGC+AGG | 0.514718 | 7.4:+1227021 | None:intergenic |
| AGATCGGAGTCGGAGCTGAA+TGG | 0.523502 | 7.4:-1228418 | MS.gene060380:CDS |
| CAGTTCTGAGGCATATCCAC+CGG | 0.547208 | 7.4:-1227016 | MS.gene060380:CDS |
| CTCCGATCTGTTACAGAGAT+CGG | 0.548830 | 7.4:+1228432 | None:intergenic |
| TCCGGGGAGAGATGAATGTA+TGG | 0.550714 | 7.4:+1228752 | None:intergenic |
| TTCAATTTGTAGGAGAAGGA+AGG | 0.557963 | 7.4:-1228282 | MS.gene060380:intron |
| CACCACTCTTATCATTCCGC+TGG | 0.561657 | 7.4:+1227481 | None:intergenic |
| CAGAGTCTAACAATAGGAAG+AGG | 0.562689 | 7.4:-1228471 | MS.gene060380:CDS |
| TTGCACATATTCCGATCAGA+TGG | 0.572786 | 7.4:-1228575 | MS.gene060380:CDS |
| TCCATACATTCATCTCTCCC+CGG | 0.577706 | 7.4:-1228753 | MS.gene060380:CDS |
| TTGGATTTGCAGGAAAAGGA+AGG | 0.578185 | 7.4:-1227862 | MS.gene060380:intron |
| TTACAATTACCCAGACAAAA+CGG | 0.582664 | 7.4:+1227236 | None:intergenic |
| ATGACATCTACCATCAGCAC+AGG | 0.592506 | 7.4:+1227188 | None:intergenic |
| CACCGATCTCTGTAACAGAT+CGG | 0.592544 | 7.4:-1228434 | MS.gene060380:CDS |
| GAGTCGAAGATACCAAACTA+CGG | 0.598607 | 7.4:-1227807 | MS.gene060380:CDS |
| GCTTCGCCACCGCTTTAGGA+CGG | 0.604737 | 7.4:+1228536 | None:intergenic |
| TTCCGTCGACCGTCCTAAAG+CGG | 0.608497 | 7.4:-1228545 | MS.gene060380:CDS |
| TATGATGATGCAGCGTGCGT+GGG | 0.611031 | 7.4:+1228776 | None:intergenic |
| CTAAAGCCCTGCCAGTTCTG+AGG | 0.614494 | 7.4:-1227028 | MS.gene060380:CDS |
| CGTCGACCGTCCTAAAGCGG+TGG | 0.619111 | 7.4:-1228542 | MS.gene060380:CDS |
| AGTCGAAGATACCAAACTAC+GGG | 0.619899 | 7.4:-1227806 | MS.gene060380:intron |
| AAAGGAAGGTAGCTGTAACA+AGG | 0.620730 | 7.4:-1227848 | MS.gene060380:CDS |
| TCTCTGTAACAGATCGGAGT+CGG | 0.622689 | 7.4:-1228428 | MS.gene060380:CDS |
| CACCGCTTTAGGACGGTCGA+CGG | 0.628927 | 7.4:+1228543 | None:intergenic |
| GGAAGAAGAGTCCATCTGAT+CGG | 0.632670 | 7.4:+1228564 | None:intergenic |
| AGTTCTGAGGCATATCCACC+GGG | 0.638449 | 7.4:-1227015 | MS.gene060380:CDS |
| GAAAGTGTGTTATCTTTCCG+GGG | 0.641011 | 7.4:+1228736 | None:intergenic |
| GATATGCCTCAGAACTGGCA+GGG | 0.647810 | 7.4:+1227022 | None:intergenic |
| CAGCCGCTGTAATTTGACAC+TGG | 0.648961 | 7.4:+1227064 | None:intergenic |
| TGATGTGTCATCAGTGCCAG+CGG | 0.652912 | 7.4:-1227497 | MS.gene060380:CDS |
| ATATGATGATGCAGCGTGCG+TGG | 0.665872 | 7.4:+1228775 | None:intergenic |
| TTGACATTAATCAGGTACCC+AGG | 0.674787 | 7.4:-1227290 | MS.gene060380:intron |
| GTTCCAAGCTCTGTTCCCGG+TGG | 0.675496 | 7.4:+1227000 | None:intergenic |
| TGATCGGAATATGTGCAATG+AGG | 0.682294 | 7.4:+1228580 | None:intergenic |
| TGCCAGCGGAATGATAAGAG+TGG | 0.697971 | 7.4:-1227483 | MS.gene060380:CDS |
| ATGCCAGTGTCAAATTACAG+CGG | 0.733329 | 7.4:-1227067 | MS.gene060380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAATAATTCAATTCAC+TGG | - | chr7.4:1227133-1227152 | MS.gene060380:intron | 15.0% |
| !! | AATAATTCAATTCAATTTGT+AGG | - | chr7.4:1227481-1227500 | MS.gene060380:CDS | 15.0% |
| !! | TTAATATTGTTTAATTACGA+AGG | - | chr7.4:1228178-1228197 | MS.gene060380:intron | 15.0% |
| !!! | ATAAACATTCATAAATCTTT+TGG | - | chr7.4:1227680-1227699 | MS.gene060380:intron | 15.0% |
| !!! | ATTAATTGTAGTTTTATTTG+AGG | - | chr7.4:1227743-1227762 | MS.gene060380:intron | 15.0% |
| !!! | GATTTCAATATTTTAGAAAA+TGG | - | chr7.4:1228059-1228078 | MS.gene060380:intron | 15.0% |
| !! | AACATTATGGTAATAATCAA+AGG | + | chr7.4:1228212-1228231 | None:intergenic | 20.0% |
| !!! | AACTTTCTTTTCTTTAAGAA+TGG | - | chr7.4:1228376-1228395 | MS.gene060380:intron | 20.0% |
| !!! | ATGGACATTTATAAATCTTT+TGG | - | chr7.4:1228078-1228097 | MS.gene060380:intron | 20.0% |
| !!! | TGGACATTTATAAATCTTTT+GGG | - | chr7.4:1228079-1228098 | MS.gene060380:intron | 20.0% |
| ! | AAGGTTAACAAAGCTAAAAA+TGG | - | chr7.4:1227944-1227963 | MS.gene060380:intron | 25.0% |
| ! | AATAATTCAATTGGATTTGC+AGG | - | chr7.4:1227901-1227920 | MS.gene060380:intron | 25.0% |
| ! | CAATTGAATTATTGTGTGTT+CGG | + | chr7.4:1227894-1227913 | None:intergenic | 25.0% |
| ! | TCTACTATATTTATAGTACG+TGG | + | chr7.4:1227078-1227097 | None:intergenic | 25.0% |
| !!! | CATATAAAGTTGTTGATGAT+TGG | - | chr7.4:1228030-1228049 | MS.gene060380:intron | 25.0% |
| AATTGAAGATACCAAACAAC+TGG | - | chr7.4:1227568-1227587 | MS.gene060380:intron | 30.0% | |
| ATATCCAAAAAAGCCAAGAA+GGG | - | chr7.4:1228245-1228264 | MS.gene060380:CDS | 30.0% | |
| CATGTACTAACTTGATGAAT+CGG | + | chr7.4:1227409-1227428 | None:intergenic | 30.0% | |
| CGAACACACAATAATTCAAT+TGG | - | chr7.4:1227892-1227911 | MS.gene060380:intron | 30.0% | |
| GGTATTATTCACATGTATGT+TGG | - | chr7.4:1228143-1228162 | MS.gene060380:intron | 30.0% | |
| TATATCCAAAAAAGCCAAGA+AGG | - | chr7.4:1228244-1228263 | MS.gene060380:CDS | 30.0% | |
| TCAATTCAATTTGTAGGAGA+AGG | - | chr7.4:1227487-1227506 | MS.gene060380:CDS | 30.0% | |
| TTACAATTACCCAGACAAAA+CGG | + | chr7.4:1228540-1228559 | None:intergenic | 30.0% | |
| ! | GGTATCTTCAATTCCATTTT+TGG | + | chr7.4:1227561-1227580 | None:intergenic | 30.0% |
| ! | GTATTGTGTATCGATGTATT+CGG | - | chr7.4:1228122-1228141 | MS.gene060380:intron | 30.0% |
| ! | TTGAAAGTGTGTTATCTTTC+CGG | + | chr7.4:1227042-1227061 | None:intergenic | 30.0% |
| !!! | ACCATAATGTTGTTTTCTCA+GGG | - | chr7.4:1228221-1228240 | MS.gene060380:CDS | 30.0% |
| !!! | ACTCTTTTCCTTGTTTTCAT+AGG | - | chr7.4:1228671-1228690 | MS.gene060380:intron | 30.0% |
| !!! | TACCATAATGTTGTTTTCTC+AGG | - | chr7.4:1228220-1228239 | MS.gene060380:CDS | 30.0% |
| AAGATGCAGAGTCTAACAAT+AGG | - | chr7.4:1227296-1227315 | MS.gene060380:intron | 35.0% | |
| AAGGTCAACAAAGCCAAAAA+TGG | - | chr7.4:1227545-1227564 | MS.gene060380:intron | 35.0% | |
| ACCCTGAGAAAACAACATTA+TGG | + | chr7.4:1228225-1228244 | None:intergenic | 35.0% | |
| CAAGAGATGACACTGAATTA+TGG | + | chr7.4:1228009-1228028 | None:intergenic | 35.0% | |
| CACTGTGATTGACATTAATC+AGG | - | chr7.4:1228475-1228494 | MS.gene060380:CDS | 35.0% | |
| CCAGTATTGTGGATCAATAA+GGG | + | chr7.4:1228446-1228465 | None:intergenic | 35.0% | |
| GTTTCAGAATAAACGTGTGT+TGG | - | chr7.4:1227715-1227734 | MS.gene060380:intron | 35.0% | |
| TCAATTGGATTTGCAGGAAA+AGG | - | chr7.4:1227907-1227926 | MS.gene060380:intron | 35.0% | |
| TTCAATTTGTAGGAGAAGGA+AGG | - | chr7.4:1227491-1227510 | MS.gene060380:CDS | 35.0% | |
| TTTCTTCTTCTTCTTTCTCG+TGG | + | chr7.4:1227386-1227405 | None:intergenic | 35.0% | |
| ! | TGAAAGTGTGTTATCTTTCC+GGG | + | chr7.4:1227041-1227060 | None:intergenic | 35.0% |
| ! | TTTTCTTCTCACCAGTTGTT+TGG | + | chr7.4:1227582-1227601 | None:intergenic | 35.0% |
| !! | CACACGTTTATTCTGAAACA+AGG | + | chr7.4:1227714-1227733 | None:intergenic | 35.0% |
| AAAGGAAGGTAGCTGTAACA+AGG | - | chr7.4:1227925-1227944 | MS.gene060380:intron | 40.0% | |
| ACACGTTCTCAAATTCCTCT+CGG | + | chr7.4:1228515-1228534 | None:intergenic | 40.0% | |
| AGTCGAAGATACCAAACTAC+GGG | - | chr7.4:1227967-1227986 | MS.gene060380:intron | 40.0% | |
| ATGCCAGTGTCAAATTACAG+CGG | - | chr7.4:1228706-1228725 | MS.gene060380:intron | 40.0% | |
| CAGAGTCTAACAATAGGAAG+AGG | - | chr7.4:1227302-1227321 | MS.gene060380:intron | 40.0% | |
| CATCAAACTTTCACCCTTCT+TGG | + | chr7.4:1228261-1228280 | None:intergenic | 40.0% | |
| CCCTTATTGATCCACAATAC+TGG | - | chr7.4:1228443-1228462 | MS.gene060380:CDS | 40.0% | |
| GAGTCGAAGATACCAAACTA+CGG | - | chr7.4:1227966-1227985 | MS.gene060380:intron | 40.0% | |
| GATCAATAAGGGAAACCAAC+TGG | + | chr7.4:1228435-1228454 | None:intergenic | 40.0% | |
| GCCAGTATTGTGGATCAATA+AGG | + | chr7.4:1228447-1228466 | None:intergenic | 40.0% | |
| GTTACGAATGCATTGAGAAC+TGG | - | chr7.4:1228351-1228370 | MS.gene060380:intron | 40.0% | |
| GTTGTCTTGAAGAAACCAGT+TGG | - | chr7.4:1228417-1228436 | MS.gene060380:CDS | 40.0% | |
| TATTGATCCACAATACTGGC+TGG | - | chr7.4:1228447-1228466 | MS.gene060380:CDS | 40.0% | |
| TGATCGGAATATGTGCAATG+AGG | + | chr7.4:1227196-1227215 | None:intergenic | 40.0% | |
| TGCAATGAGGAATCTGATGA+TGG | + | chr7.4:1227183-1227202 | None:intergenic | 40.0% | |
| TTCTCGGTCCTATGAAAACA+AGG | + | chr7.4:1228682-1228701 | None:intergenic | 40.0% | |
| TTGACATTAATCAGGTACCC+AGG | - | chr7.4:1228483-1228502 | MS.gene060380:CDS | 40.0% | |
| TTGCACATATTCCGATCAGA+TGG | - | chr7.4:1227198-1227217 | MS.gene060380:intron | 40.0% | |
| TTGGATTTGCAGGAAAAGGA+AGG | - | chr7.4:1227911-1227930 | MS.gene060380:intron | 40.0% | |
| ! | CTGCATCTTCAATTTTGCTG+TGG | + | chr7.4:1227285-1227304 | None:intergenic | 40.0% |
| ! | GAAAGTGTGTTATCTTTCCG+GGG | + | chr7.4:1227040-1227059 | None:intergenic | 40.0% |
| ! | GGGACTAATGGATGTGTATA+AGG | - | chr7.4:1227526-1227545 | MS.gene060380:CDS | 40.0% |
| ! | GTAACTGCAAAGCTTGCTTA+CGG | - | chr7.4:1228556-1228575 | MS.gene060380:CDS | 40.0% |
| ! | TAACTGCAAAGCTTGCTTAC+GGG | - | chr7.4:1228557-1228576 | MS.gene060380:CDS | 40.0% |
| ! | TAGGAGAAGGAAGGTGATTT+TGG | - | chr7.4:1227500-1227519 | MS.gene060380:CDS | 40.0% |
| ! | TGACACTGGCATCTATTTCT+CGG | + | chr7.4:1228698-1228717 | None:intergenic | 40.0% |
| !! | AAGGAAGGTGATTTTGGCAA+GGG | - | chr7.4:1227506-1227525 | MS.gene060380:CDS | 40.0% |
| !! | AATTTTGCTGTGGTTCTCTC+CGG | + | chr7.4:1227275-1227294 | None:intergenic | 40.0% |
| !! | GGAATCTGATGATGGAGAAT+AGG | + | chr7.4:1227175-1227194 | None:intergenic | 40.0% |
| !! | TGATTTTGGCAAGGGACTAA+TGG | - | chr7.4:1227514-1227533 | MS.gene060380:CDS | 40.0% |
| !!! | TTCACCCTTCTTGGCTTTTT+TGG | + | chr7.4:1228252-1228271 | None:intergenic | 40.0% |
| ATGACATCTACCATCAGCAC+AGG | + | chr7.4:1228588-1228607 | None:intergenic | 45.0% | |
| CACCGATCTCTGTAACAGAT+CGG | - | chr7.4:1227339-1227358 | MS.gene060380:intron | 45.0% | |
| CACGTTCTCAAATTCCTCTC+GGG | + | chr7.4:1228514-1228533 | None:intergenic | 45.0% | |
| CTCCGATCTGTTACAGAGAT+CGG | + | chr7.4:1227344-1227363 | None:intergenic | 45.0% | |
| GGAAGAAGAGTCCATCTGAT+CGG | + | chr7.4:1227212-1227231 | None:intergenic | 45.0% | |
| TCCATACATTCATCTCTCCC+CGG | - | chr7.4:1227020-1227039 | MS.gene060380:CDS | 45.0% | |
| TCTCTGTAACAGATCGGAGT+CGG | - | chr7.4:1227345-1227364 | MS.gene060380:intron | 45.0% | |
| TTCTCTTCTCACCCGTAGTT+TGG | + | chr7.4:1227981-1228000 | None:intergenic | 45.0% | |
| ! | AACGTGTGTCCGTTTTGTCT+GGG | - | chr7.4:1228528-1228547 | MS.gene060380:CDS | 45.0% |
| ! | AATTCCTCTCGGGTTTTTCC+TGG | + | chr7.4:1228504-1228523 | None:intergenic | 45.0% |
| ! | ATTCCTCTCGGGTTTTTCCT+GGG | + | chr7.4:1228503-1228522 | None:intergenic | 45.0% |
| !! | GAAGGAAGGTGATTTTGGCA+AGG | - | chr7.4:1227505-1227524 | MS.gene060380:CDS | 45.0% |
| !!! | ATTTATTTTTATAATATGAT+TGG | + | chr7.4:1227121-1227140 | None:intergenic | 5.0% |
| CACCACTCTTATCATTCCGC+TGG | + | chr7.4:1228295-1228314 | None:intergenic | 50.0% | |
| CAGCCGCTGTAATTTGACAC+TGG | + | chr7.4:1228712-1228731 | None:intergenic | 50.0% | |
| CTAGTTCCAAGCTCTGTTCC+CGG | + | chr7.4:1228779-1228798 | None:intergenic | 50.0% | |
| GATATGCCTCAGAACTGGCA+GGG | + | chr7.4:1228754-1228773 | None:intergenic | 50.0% | |
| TCCGGGGAGAGATGAATGTA+TGG | + | chr7.4:1227024-1227043 | None:intergenic | 50.0% | |
| TGAGTTGCCAGCCAGTATTG+TGG | + | chr7.4:1228457-1228476 | None:intergenic | 50.0% | |
| TGATGTGTCATCAGTGCCAG+CGG | - | chr7.4:1228276-1228295 | MS.gene060380:intron | 50.0% | |
| TGCCAGCGGAATGATAAGAG+TGG | - | chr7.4:1228290-1228309 | MS.gene060380:intron | 50.0% | |
| ! | ATATGATGATGCAGCGTGCG+TGG | + | chr7.4:1227001-1227020 | None:intergenic | 50.0% |
| ! | GAACGTGTGTCCGTTTTGTC+TGG | - | chr7.4:1228527-1228546 | MS.gene060380:CDS | 50.0% |
| ! | TATGATGATGCAGCGTGCGT+GGG | + | chr7.4:1227000-1227019 | None:intergenic | 50.0% |
| !! | AGTTCTGAGGCATATCCACC+GGG | - | chr7.4:1228758-1228777 | MS.gene060380:CDS | 50.0% |
| !! | CAGTTCTGAGGCATATCCAC+CGG | - | chr7.4:1228757-1228776 | MS.gene060380:CDS | 50.0% |
| ACGGGAGTTTCCTGTGCTGA+TGG | - | chr7.4:1228575-1228594 | MS.gene060380:CDS | 55.0% | |
| AGATCGGAGTCGGAGCTGAA+TGG | - | chr7.4:1227355-1227374 | MS.gene060380:intron | 55.0% | |
| CGGTGGATATGCCTCAGAAC+TGG | + | chr7.4:1228759-1228778 | None:intergenic | 55.0% | |
| CTAAAGCCCTGCCAGTTCTG+AGG | - | chr7.4:1228745-1228764 | MS.gene060380:CDS | 55.0% | |
| GGATATGCCTCAGAACTGGC+AGG | + | chr7.4:1228755-1228774 | None:intergenic | 55.0% | |
| GTACCCAGGAAAAACCCGAG+AGG | - | chr7.4:1228497-1228516 | MS.gene060380:CDS | 55.0% | |
| TATCCACCGGGAACAGAGCT+TGG | - | chr7.4:1228770-1228789 | MS.gene060380:CDS | 55.0% | |
| TTCCGTCGACCGTCCTAAAG+CGG | - | chr7.4:1227228-1227247 | MS.gene060380:CDS | 55.0% | |
| GTTCCAAGCTCTGTTCCCGG+TGG | + | chr7.4:1228776-1228795 | None:intergenic | 60.0% | |
| !! | CACCGCTTTAGGACGGTCGA+CGG | + | chr7.4:1227233-1227252 | None:intergenic | 60.0% |
| !! | GCTTCGCCACCGCTTTAGGA+CGG | + | chr7.4:1227240-1227259 | None:intergenic | 60.0% |
| CGTCGACCGTCCTAAAGCGG+TGG | - | chr7.4:1227231-1227250 | MS.gene060380:CDS | 65.0% | |
| GCACGCTTCGCCACCGCTTT+AGG | + | chr7.4:1227244-1227263 | None:intergenic | 65.0% | |
| GTGGTTCTCTCCGGCGACGT+TGG | + | chr7.4:1227266-1227285 | None:intergenic | 65.0% | |
| GCGAAGCGTGCCAACGTCGC+CGG | - | chr7.4:1227253-1227272 | MS.gene060380:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 1226980 | 1228815 | 1226980 | ID=MS.gene060380 |
| chr7.4 | mRNA | 1226980 | 1228815 | 1226980 | ID=MS.gene060380.t1;Parent=MS.gene060380 |
| chr7.4 | exon | 1228717 | 1228815 | 1228717 | ID=MS.gene060380.t1.exon1;Parent=MS.gene060380.t1 |
| chr7.4 | CDS | 1228717 | 1228815 | 1228717 | ID=cds.MS.gene060380.t1;Parent=MS.gene060380.t1 |
| chr7.4 | exon | 1228378 | 1228605 | 1228378 | ID=MS.gene060380.t1.exon2;Parent=MS.gene060380.t1 |
| chr7.4 | CDS | 1228378 | 1228605 | 1228378 | ID=cds.MS.gene060380.t1;Parent=MS.gene060380.t1 |
| chr7.4 | exon | 1228206 | 1228292 | 1228206 | ID=MS.gene060380.t1.exon3;Parent=MS.gene060380.t1 |
| chr7.4 | CDS | 1228206 | 1228292 | 1228206 | ID=cds.MS.gene060380.t1;Parent=MS.gene060380.t1 |
| chr7.4 | exon | 1227807 | 1227872 | 1227807 | ID=MS.gene060380.t1.exon4;Parent=MS.gene060380.t1 |
| chr7.4 | CDS | 1227807 | 1227872 | 1227807 | ID=cds.MS.gene060380.t1;Parent=MS.gene060380.t1 |
| chr7.4 | exon | 1227423 | 1227553 | 1227423 | ID=MS.gene060380.t1.exon5;Parent=MS.gene060380.t1 |
| chr7.4 | CDS | 1227423 | 1227553 | 1227423 | ID=cds.MS.gene060380.t1;Parent=MS.gene060380.t1 |
| chr7.4 | exon | 1227199 | 1227298 | 1227199 | ID=MS.gene060380.t1.exon6;Parent=MS.gene060380.t1 |
| chr7.4 | CDS | 1227199 | 1227298 | 1227199 | ID=cds.MS.gene060380.t1;Parent=MS.gene060380.t1 |
| chr7.4 | exon | 1226980 | 1227102 | 1226980 | ID=MS.gene060380.t1.exon7;Parent=MS.gene060380.t1 |
| chr7.4 | CDS | 1226980 | 1227102 | 1226980 | ID=cds.MS.gene060380.t1;Parent=MS.gene060380.t1 |
| Gene Sequence |
| Protein sequence |
