AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47447

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47447.t1 MTR_1g063020 99.324 296 2 0 1 296 1 296 0.0 617
MS.gene47447.t1 MTR_7g102260 83.162 291 49 0 6 296 3 293 0.0 522
MS.gene47447.t1 MTR_4g057450 69.550 289 88 0 6 294 16 304 6.04e-155 435
MS.gene47447.t1 MTR_2g095800 64.474 304 97 2 2 294 6 309 4.59e-145 410
MS.gene47447.t1 MTR_1g033420 40.268 298 157 7 5 294 7 291 2.07e-68 216
MS.gene47447.t1 MTR_8g064180 37.459 307 175 6 1 294 8 310 3.16e-66 209
MS.gene47447.t1 MTR_1g105900 38.686 274 149 8 32 294 26 291 4.06e-61 196
MS.gene47447.t1 MTR_5g029100 38.889 270 150 4 37 294 39 305 5.01e-61 197
MS.gene47447.t1 MTR_3g089540 41.481 270 140 7 33 294 23 282 4.25e-60 193
MS.gene47447.t1 MTR_7g093530 39.245 265 144 6 39 294 23 279 1.66e-58 189
MS.gene47447.t1 MTR_8g081040 40.076 262 141 6 39 294 30 281 4.51e-58 188
MS.gene47447.t1 MTR_4g128410 36.482 307 155 9 1 294 1 280 7.57e-58 187
MS.gene47447.t1 MTR_5g091730 41.597 238 125 6 61 294 51 278 1.11e-57 187
MS.gene47447.t1 MTR_7g084770 38.545 275 148 8 31 294 23 287 1.12e-57 187
MS.gene47447.t1 MTR_7g084760 37.710 297 160 10 9 294 5 287 2.77e-57 186
MS.gene47447.t1 MTR_4g128570 40.840 262 130 9 39 294 30 272 3.16e-57 185
MS.gene47447.t1 MTR_6g088320 38.760 258 139 6 48 294 34 283 4.59e-57 185
MS.gene47447.t1 MTR_4g128400 40.310 258 133 7 48 294 36 283 5.85e-57 185
MS.gene47447.t1 MTR_3g084630 40.152 264 141 6 37 294 25 277 1.53e-56 184
MS.gene47447.t1 MTR_3g084620 40.152 264 141 6 37 294 25 277 1.88e-56 184
MS.gene47447.t1 MTR_7g084750 38.290 269 145 8 37 294 29 287 9.35e-56 182
MS.gene47447.t1 MTR_8g102910 40.840 262 128 8 48 294 30 279 1.95e-55 181
MS.gene47447.t1 MTR_4g126920 37.132 272 152 7 34 294 18 281 2.18e-55 181
MS.gene47447.t1 MTR_4g045853 38.583 254 133 5 48 286 35 280 5.04e-55 180
MS.gene47447.t1 MTR_1g105495 34.768 302 165 9 9 294 9 294 9.75e-55 180
MS.gene47447.t1 MTR_6g033085 37.879 264 147 7 40 294 32 287 1.06e-54 179
MS.gene47447.t1 MTR_5g029100 39.407 236 130 3 69 294 42 274 1.37e-54 180
MS.gene47447.t1 MTR_2g089140 36.000 275 153 5 35 294 20 286 1.50e-54 179
MS.gene47447.t1 MTR_4g128580 37.410 278 153 6 30 294 16 285 2.07e-54 178
MS.gene47447.t1 MTR_2g038860 36.667 270 132 7 39 286 25 277 8.69e-54 177
MS.gene47447.t1 MTR_2g038705 36.667 270 132 7 39 286 25 277 8.69e-54 177
MS.gene47447.t1 MTR_6g033055 37.500 264 148 7 40 294 32 287 8.73e-54 177
MS.gene47447.t1 MTR_8g102220 37.023 262 136 8 39 294 28 266 4.29e-51 169
MS.gene47447.t1 MTR_8g090100 36.727 275 150 7 35 294 16 281 4.30e-51 171
MS.gene47447.t1 MTR_8g013055 37.736 265 141 8 34 286 25 277 2.45e-50 168
MS.gene47447.t1 MTR_7g056090 37.248 298 150 11 7 294 11 281 3.74e-50 167
MS.gene47447.t1 MTR_4g097640 36.194 268 154 7 36 294 28 287 8.59e-50 167
MS.gene47447.t1 MTR_4g097670 36.194 268 154 7 36 294 28 287 8.59e-50 167
MS.gene47447.t1 MTR_2g089140 37.991 229 119 5 81 294 3 223 2.47e-49 164
MS.gene47447.t1 MTR_4g097700 36.226 265 152 7 39 294 68 324 3.63e-49 166
MS.gene47447.t1 MTR_4g128590 40.455 220 111 6 87 294 64 275 9.19e-49 164
MS.gene47447.t1 MTR_2g049030 32.342 269 156 11 35 294 25 276 5.99e-37 133
MS.gene47447.t1 MTR_2g049130 32.353 272 154 12 37 296 26 279 1.88e-36 132
MS.gene47447.t1 MTR_8g032640 33.333 174 99 6 65 234 38 198 6.58e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47447.t1 AT2G36870 81.443 291 54 0 5 295 8 298 0.0 506
MS.gene47447.t1 AT3G44990 64.966 294 102 1 1 294 1 293 6.01e-140 397
MS.gene47447.t1 AT2G36870 80.117 171 34 0 5 175 8 178 3.26e-101 294
MS.gene47447.t1 AT1G10550 35.714 308 171 6 6 294 10 309 2.04e-65 207
MS.gene47447.t1 AT1G14720 39.405 269 152 5 34 294 25 290 1.75e-64 206
MS.gene47447.t1 AT4G14130 41.791 268 134 7 39 294 27 284 6.20e-64 203
MS.gene47447.t1 AT1G32170 40.293 273 148 5 33 294 25 293 4.14e-62 200
MS.gene47447.t1 AT5G57550 38.246 285 154 6 7 286 6 273 4.63e-62 198
MS.gene47447.t1 AT2G01850 40.152 264 147 5 39 294 30 290 3.73e-61 197
MS.gene47447.t1 AT5G57530 40.370 270 142 8 35 294 22 282 1.07e-60 194
MS.gene47447.t1 AT3G23730 39.852 271 142 7 37 294 24 286 5.06e-60 193
MS.gene47447.t1 AT1G11545 37.500 312 151 10 2 294 13 299 1.07e-58 190
MS.gene47447.t1 AT5G57540 40.000 265 142 8 39 294 25 281 6.30e-58 187
MS.gene47447.t1 AT4G18990 37.193 285 151 6 33 294 33 312 1.57e-57 189
MS.gene47447.t1 AT5G57560 39.483 271 142 9 37 294 20 281 3.00e-57 186
MS.gene47447.t1 AT5G48070 37.377 305 146 10 2 294 10 281 1.15e-56 184
MS.gene47447.t1 AT4G30290 40.000 300 141 10 7 294 4 276 2.14e-56 183
MS.gene47447.t1 AT3G25050 36.466 266 153 5 38 295 33 290 2.15e-56 184
MS.gene47447.t1 AT4G25810 40.000 270 141 9 37 294 23 283 2.26e-56 184
MS.gene47447.t1 AT4G25820 41.729 266 136 9 39 294 29 285 2.40e-56 183
MS.gene47447.t1 AT4G30280 44.681 235 107 7 69 294 61 281 7.03e-56 182
MS.gene47447.t1 AT1G65310 44.589 231 113 7 69 294 61 281 1.65e-55 181
MS.gene47447.t1 AT1G65310 44.589 231 113 7 69 294 83 303 2.43e-55 181
MS.gene47447.t1 AT4G30270 37.037 297 147 9 4 294 3 265 6.36e-55 179
MS.gene47447.t1 AT5G13870 36.630 273 156 7 31 294 23 287 1.15e-54 179
MS.gene47447.t1 AT5G13870 36.630 273 156 7 31 294 23 287 1.15e-54 179
MS.gene47447.t1 AT5G13870 36.630 273 156 7 31 294 23 287 1.15e-54 179
MS.gene47447.t1 AT2G14620 36.093 302 166 10 4 294 9 294 1.34e-54 179
MS.gene47447.t1 AT4G03210 36.194 268 140 5 35 286 24 276 1.52e-54 179
MS.gene47447.t1 AT5G65730 35.235 298 160 8 6 294 17 290 3.04e-54 178
MS.gene47447.t1 AT4G37800 38.806 268 147 6 36 294 30 289 4.31e-54 178
MS.gene47447.t1 AT4G13080 35.880 301 173 8 1 294 4 291 6.09e-54 177
MS.gene47447.t1 AT2G06850 38.267 277 146 9 31 294 26 290 2.97e-53 176
MS.gene47447.t1 AT2G18800 36.803 269 140 8 48 294 36 296 1.61e-52 174
MS.gene47447.t1 AT4G13090 36.333 300 164 7 5 294 6 288 2.85e-52 173
MS.gene47447.t1 AT2G06850 40.336 238 119 8 68 294 5 230 2.42e-50 166
MS.gene47447.t1 AT4G03210 36.800 250 124 6 61 294 25 256 3.36e-49 164
MS.gene47447.t1 AT2G14620 37.872 235 129 7 69 294 18 244 4.26e-49 164
MS.gene47447.t1 AT4G28850 35.581 267 152 5 40 294 29 287 2.24e-48 163
MS.gene47447.t1 AT2G14620 37.991 229 125 7 75 294 13 233 1.54e-47 159
MS.gene47447.t1 AT4G18990 38.208 212 105 5 104 294 32 238 5.77e-42 146
MS.gene47447.t1 AT3G48580 34.082 267 133 10 42 294 36 273 5.33e-40 141
MS.gene47447.t1 AT3G48580 34.034 238 119 8 68 294 4 214 2.03e-36 130
MS.gene47447.t1 AT3G48580 29.963 267 124 11 42 294 36 253 1.82e-24 99.8

Find 91 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGACCAATGTGGGTTTA+TGG 0.178750 1.4:-51854294 MS.gene47447:CDS
CTTCCTTGGGTACCTCCTTA+TGG 0.183924 1.4:+51854335 None:intergenic
GGCCACCTTCCCCTGGTTAC+TGG 0.192690 1.4:-51856162 MS.gene47447:CDS
TGTGGGTTTATGGTTCTATT+TGG 0.204208 1.4:-51854284 MS.gene47447:CDS
ACTATTGTCAAGATCCTAAA+AGG 0.217459 1.4:-51854044 MS.gene47447:CDS
ATCAACATGGAACAACAATT+TGG 0.259205 1.4:-51856063 MS.gene47447:CDS
TTCCTTGGGTACCTCCTTAT+GGG 0.266893 1.4:+51854336 None:intergenic
TTCATCACTATGCTATAGTT+TGG 0.290387 1.4:-51855415 MS.gene47447:CDS
CAATGGTTATAGAAACCTTT+GGG 0.298310 1.4:-51856107 MS.gene47447:CDS
AAGATTTAACCCCAACATTC+AGG 0.298689 1.4:+51854006 None:intergenic
ACATTTGTCTGCAAAGTATA+AGG 0.303238 1.4:+51855518 None:intergenic
GAAGTAGACATTGAATTTCT+AGG 0.341397 1.4:-51855557 MS.gene47447:CDS
CTGCTTCAAGCAATGCCTAT+TGG 0.352937 1.4:-51856183 MS.gene47447:CDS
GTGGGTTTATGGTTCTATTT+GGG 0.354310 1.4:-51854283 MS.gene47447:CDS
CAGACAAATGTTTATATAAG+AGG 0.361231 1.4:-51855506 MS.gene47447:CDS
TAGACTAACACCTGAATGTT+GGG 0.370136 1.4:-51854016 MS.gene47447:CDS
AACAATGTACAGATTCTTAG+TGG 0.388025 1.4:-51854367 MS.gene47447:intron
GAATTTCTAGGGACAACATT+TGG 0.388283 1.4:-51855545 MS.gene47447:CDS
TTCCATGTTGATCCATGCTT+TGG 0.388299 1.4:+51856074 None:intergenic
AATGTTGCATCACTCTTCCT+TGG 0.393842 1.4:+51854321 None:intergenic
GTGACTTACATTAATTCCTT+AGG 0.405222 1.4:+51855389 None:intergenic
ACAATGGTTATAGAAACCTT+TGG 0.410802 1.4:-51856108 MS.gene47447:CDS
AACATTCAGGTGTTAGTCTA+TGG 0.416142 1.4:+51854019 None:intergenic
TTTGTGTACTTTGCAACAAA+AGG 0.422740 1.4:+51854204 None:intergenic
TAAACCCACATTGGTCTTAG+TGG 0.431879 1.4:+51854297 None:intergenic
CCATTTCGATCGGGATACTT+TGG 0.438416 1.4:-51855785 MS.gene47447:CDS
CGGCCACGGCCTCGCCGTAT+CGG 0.441815 1.4:-51854134 MS.gene47447:CDS
TGCATCACGGTGGTGTCGTC+CGG 0.448578 1.4:-51854154 MS.gene47447:CDS
GCCTATTGGCCACCTTCCCC+TGG 0.450220 1.4:-51856169 MS.gene47447:CDS
ACAACATTGGTCTATGAGTT+GGG 0.459656 1.4:-51854091 MS.gene47447:CDS
CCAAAGTATCCCGATCGAAA+TGG 0.463082 1.4:+51855785 None:intergenic
ACCAGGGGAAGGTGGCCAAT+AGG 0.463800 1.4:+51856168 None:intergenic
AACAACATTGGTCTATGAGT+TGG 0.476903 1.4:-51854092 MS.gene47447:CDS
ATGTTGCATCACTCTTCCTT+GGG 0.478571 1.4:+51854322 None:intergenic
TCATCATGGGCAACAGAAGA+TGG 0.483074 1.4:-51854255 MS.gene47447:CDS
ACGGTGGTGTCGTCCGGCCA+CGG 0.487364 1.4:-51854148 MS.gene47447:CDS
TCTAACAATGAAGCGCATCC+AGG 0.493067 1.4:-51855590 MS.gene47447:CDS
TTGGAACTTGGCCAGTAACC+AGG 0.493648 1.4:+51856151 None:intergenic
TCTACTTCATCATGAAATCC+TGG 0.494735 1.4:+51855572 None:intergenic
CTATTTGGGATGCTTCATCA+TGG 0.504142 1.4:-51854269 MS.gene47447:CDS
ATAGACCTGACTTTGGAACT+TGG 0.504684 1.4:+51856139 None:intergenic
ATTTGGCTTGATAGAACCTC+AGG 0.506453 1.4:-51856046 MS.gene47447:intron
ATAACTCCTGCAGTGTAGCC+AGG 0.507404 1.4:+51855743 None:intergenic
GCCTCGCCGTATCGGTCCGG+TGG 0.507497 1.4:-51854126 MS.gene47447:CDS
AAGTAGACATTGAATTTCTA+GGG 0.508075 1.4:-51855556 MS.gene47447:CDS
ACTGGCCAAGTTCCAAAGTC+AGG 0.515213 1.4:-51856144 MS.gene47447:CDS
ACGGCCTCGCCGTATCGGTC+CGG 0.518193 1.4:-51854129 MS.gene47447:CDS
TATATAAGAGGGAGTGGTGA+TGG 0.520979 1.4:-51855494 MS.gene47447:CDS
CTTCATCCTGGCTACACTGC+AGG 0.522870 1.4:-51855749 MS.gene47447:CDS
TAGTGGATGATATGCCCATA+AGG 0.525035 1.4:-51854350 MS.gene47447:CDS
CGATACGGCGAGGCCGTGGC+CGG 0.537351 1.4:+51854135 None:intergenic
GTCGAATCGTCCATTTCGAT+CGG 0.539068 1.4:-51855795 MS.gene47447:CDS
AATGTTTATATAAGAGGGAG+TGG 0.539977 1.4:-51855500 MS.gene47447:CDS
GAGAAATGAAATTTCATCTG+TGG 0.540326 1.4:-51855457 MS.gene47447:CDS
AAAGCTCATAGACCTGACTT+TGG 0.542912 1.4:+51856132 None:intergenic
AGACAAATGTTTATATAAGA+GGG 0.545157 1.4:-51855505 MS.gene47447:CDS
TGATCCATGCTTTGGTGCTG+AGG 0.547528 1.4:+51856082 None:intergenic
TATTTGGGATGCTTCATCAT+GGG 0.549893 1.4:-51854268 MS.gene47447:CDS
TGCTATAGTTTGGAATCCTA+AGG 0.550228 1.4:-51855405 MS.gene47447:CDS
TCGAATCGTCCATTTCGATC+GGG 0.552144 1.4:-51855794 MS.gene47447:CDS
GGACCGATACGGCGAGGCCG+TGG 0.552441 1.4:+51854131 None:intergenic
ATTTCCACTAAGACCAATGT+GGG 0.553604 1.4:-51854301 MS.gene47447:CDS
TGTACTTTGCAACAAAAGGT+TGG 0.556484 1.4:+51854208 None:intergenic
ATAGACTAACACCTGAATGT+TGG 0.559466 1.4:-51854017 MS.gene47447:CDS
GGCCAGTAACCAGGGGAAGG+TGG 0.570410 1.4:+51856160 None:intergenic
GTCAACCCACCGGACCGATA+CGG 0.571686 1.4:+51854120 None:intergenic
ACGACACCACCGTGATGCAT+AGG 0.586001 1.4:+51854158 None:intergenic
TGGAACTTGGCCAGTAACCA+GGG 0.586133 1.4:+51856152 None:intergenic
GGGTCCTCAGCACCAAAGCA+TGG 0.591378 1.4:-51856086 MS.gene47447:CDS
GCTTCGATTAAACTTCATCC+TGG 0.593516 1.4:-51855761 MS.gene47447:CDS
CTATTGTCAAGATCCTAAAA+GGG 0.595880 1.4:-51854043 MS.gene47447:CDS
CCTCGCCGTATCGGTCCGGT+GGG 0.599396 1.4:-51854125 MS.gene47447:CDS
CTTGGCCAGTAACCAGGGGA+AGG 0.603822 1.4:+51856157 None:intergenic
TGCCCATAAGGAGGTACCCA+AGG 0.604081 1.4:-51854338 MS.gene47447:CDS
TACACAAACTTCAAAGCTAG+TGG 0.609014 1.4:-51854189 MS.gene47447:CDS
TTGTTGTCTTGTCAACCCAC+CGG 0.610498 1.4:+51854110 None:intergenic
TTTGGTGCTGAGGACCCCAA+AGG 0.620328 1.4:+51856092 None:intergenic
TTGGGTTCAAAGACACCACA+TGG 0.630330 1.4:-51854073 MS.gene47447:CDS
CACCAAAGCATGGATCAACA+TGG 0.631601 1.4:-51856076 MS.gene47447:CDS
CTAGCAAAGAGAAATATGAA+AGG 0.636720 1.4:+51856220 None:intergenic
GGAACTTGGCCAGTAACCAG+GGG 0.650744 1.4:+51856153 None:intergenic
GGTTGACAAGACAACAACAT+TGG 0.653516 1.4:-51854104 MS.gene47447:CDS
AATGGTTATAGAAACCTTTG+GGG 0.656393 1.4:-51856106 MS.gene47447:CDS
ATAGAACCATAAACCCACAT+TGG 0.658123 1.4:+51854288 None:intergenic
CATTTCCACTAAGACCAATG+TGG 0.658778 1.4:-51854302 MS.gene47447:CDS
GTTGCACAGCCTATGCATCA+CGG 0.660505 1.4:-51854167 MS.gene47447:CDS
AGACTAACACCTGAATGTTG+GGG 0.661530 1.4:-51854015 MS.gene47447:CDS
GACAATAGTTATAAACCATG+TGG 0.677923 1.4:+51854058 None:intergenic
TGGATGATATGCCCATAAGG+AGG 0.688479 1.4:-51854347 MS.gene47447:CDS
CCCACCGGACCGATACGGCG+AGG 0.695099 1.4:+51854125 None:intergenic
GCACAGCCTATGCATCACGG+TGG 0.776007 1.4:-51854164 MS.gene47447:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAACTAATTATATATAATA+AGG + chr1.4:51855739-51855758 None:intergenic 10.0%
!! GATAAATAAATGAAATAAAA+TGG + chr1.4:51855178-51855197 None:intergenic 10.0%
!! TTATTTCATTTATTTATCAT+CGG - chr1.4:51855180-51855199 MS.gene47447:intron 10.0%
!!! TATATTTTTTTCAAAGAAAA+TGG + chr1.4:51855439-51855458 None:intergenic 10.0%
!!! TTTTTTTTATTTCAATCTAA+TGG - chr1.4:51855637-51855656 MS.gene47447:intron 10.0%
!! ATAATTAGAAAGAAAAATGT+AGG + chr1.4:51854409-51854428 None:intergenic 15.0%
!!! CATTTTTCTTTCTAATTATT+AGG - chr1.4:51854410-51854429 MS.gene47447:intron 15.0%
!! AAAAATGCAATAATGTAACT+TGG - chr1.4:51855299-51855318 MS.gene47447:intron 20.0%
!! AAAATGCAATAATGTAACTT+GGG - chr1.4:51855300-51855319 MS.gene47447:intron 20.0%
!! AGACAAATGTTTATATAAGA+GGG - chr1.4:51854735-51854754 MS.gene47447:intron 20.0%
!! TTCAAAGAAAATGGTATTAT+TGG + chr1.4:51855430-51855449 None:intergenic 20.0%
!!! AAATGAAGTTGATTAATAGA+AGG - chr1.4:51854562-51854581 MS.gene47447:intron 20.0%
!!! AGTTATTTCCAAAATTACAA+TGG - chr1.4:51854313-51854332 MS.gene47447:CDS 20.0%
!!! TGACATTTTGTAAAATGAAA+TGG - chr1.4:51855397-51855416 MS.gene47447:intron 20.0%
! AAACTATCCTTATAAACTTG+TGG + chr1.4:51855145-51855164 None:intergenic 25.0%
! AAGAAAAAATCACTTTCACA+TGG + chr1.4:51855785-51855804 None:intergenic 25.0%
! AAGTAGACATTGAATTTCTA+GGG - chr1.4:51854684-51854703 MS.gene47447:intron 25.0%
! CAGACAAATGTTTATATAAG+AGG - chr1.4:51854734-51854753 MS.gene47447:intron 25.0%
! GAAATAACCACAAGTTTATA+AGG - chr1.4:51855135-51855154 MS.gene47447:intron 25.0%
! GAGACATTTCACTTAATAAA+TGG - chr1.4:51854935-51854954 MS.gene47447:intron 25.0%
! GGAACCTTACTAATTATAAA+TGG - chr1.4:51855675-51855694 MS.gene47447:intron 25.0%
! TCAAAAAAGTAGAGCATATA+GGG - chr1.4:51855226-51855245 MS.gene47447:intron 25.0%
! TCTATCTACTGGAAAAATTA+CGG - chr1.4:51854257-51854276 MS.gene47447:CDS 25.0%
! TCTTTCTAATTATTAGGAAG+TGG - chr1.4:51854416-51854435 MS.gene47447:intron 25.0%
! TGACGATTATTATACTATGT+CGG + chr1.4:51855044-51855063 None:intergenic 25.0%
! TTAAAGTTTCAGCAAAATCA+TGG + chr1.4:51854288-51854307 None:intergenic 25.0%
! TTAAATGTGACACTCTTTAA+AGG + chr1.4:51855081-51855100 None:intergenic 25.0%
! TTCAAAAAAGTAGAGCATAT+AGG - chr1.4:51855225-51855244 MS.gene47447:intron 25.0%
! TTCAGCAGAAAAAGAAATAT+TGG + chr1.4:51855255-51855274 None:intergenic 25.0%
! TTTATTATTCCTTTCGAATC+TGG - chr1.4:51855506-51855525 MS.gene47447:CDS 25.0%
!! TTTTCTGCTGAAACATAATA+GGG - chr1.4:51855263-51855282 MS.gene47447:intron 25.0%
!! TTTTTCTGCTGAAACATAAT+AGG - chr1.4:51855262-51855281 MS.gene47447:intron 25.0%
!!! AGCATAGTGATGAAATTTTT+TGG + chr1.4:51854819-51854838 None:intergenic 25.0%
!!! AGTGCCATTTATAATTAGTA+AGG + chr1.4:51855682-51855701 None:intergenic 25.0%
!!! ATTGATTTGTCTGATTCTTA+CGG - chr1.4:51855339-51855358 MS.gene47447:intron 25.0%
!!! TAGTGATGAAATTTTTTGGT+AGG + chr1.4:51854815-51854834 None:intergenic 25.0%
!!! TGTGAAAGTGATTTTTTCTT+TGG - chr1.4:51855785-51855804 MS.gene47447:CDS 25.0%
AACAATGTACAGATTCTTAG+TGG - chr1.4:51855873-51855892 MS.gene47447:intron 30.0%
AATGGTTATAGAAACCTTTG+GGG - chr1.4:51854134-51854153 MS.gene47447:CDS 30.0%
AATGTTTATATAAGAGGGAG+TGG - chr1.4:51854740-51854759 MS.gene47447:intron 30.0%
ACAATGGTTATAGAAACCTT+TGG - chr1.4:51854132-51854151 MS.gene47447:CDS 30.0%
ACATTATATGTGGAGAAGTA+GGG + chr1.4:51855535-51855554 None:intergenic 30.0%
ACATTTGTCTGCAAAGTATA+AGG + chr1.4:51854725-51854744 None:intergenic 30.0%
ACTATTGTCAAGATCCTAAA+AGG - chr1.4:51856196-51856215 MS.gene47447:CDS 30.0%
ATCAACATGGAACAACAATT+TGG - chr1.4:51854177-51854196 MS.gene47447:CDS 30.0%
ATGTCTCTTATAGTTGAGAT+CGG + chr1.4:51854922-51854941 None:intergenic 30.0%
ATTTCCAAAATTACAATGGC+CGG - chr1.4:51854317-51854336 MS.gene47447:CDS 30.0%
CAATGGTTATAGAAACCTTT+GGG - chr1.4:51854133-51854152 MS.gene47447:CDS 30.0%
CTAATGGTCATGATATTGAA+GGG - chr1.4:51855653-51855672 MS.gene47447:intron 30.0%
CTAGCAAAGAGAAATATGAA+AGG + chr1.4:51854023-51854042 None:intergenic 30.0%
CTATTGTCAAGATCCTAAAA+GGG - chr1.4:51856197-51856216 MS.gene47447:CDS 30.0%
GAAGTAGACATTGAATTTCT+AGG - chr1.4:51854683-51854702 MS.gene47447:intron 30.0%
GACAATAGTTATAAACCATG+TGG + chr1.4:51856185-51856204 None:intergenic 30.0%
GAGAAATGAAATTTCATCTG+TGG - chr1.4:51854783-51854802 MS.gene47447:intron 30.0%
GTGACTTACATTAATTCCTT+AGG + chr1.4:51854854-51854873 None:intergenic 30.0%
TAATGGTCATGATATTGAAG+GGG - chr1.4:51855654-51855673 MS.gene47447:intron 30.0%
TCTAATGGTCATGATATTGA+AGG - chr1.4:51855652-51855671 MS.gene47447:intron 30.0%
TTCATCACTATGCTATAGTT+TGG - chr1.4:51854825-51854844 MS.gene47447:intron 30.0%
TTTGTGTACTTTGCAACAAA+AGG + chr1.4:51856039-51856058 None:intergenic 30.0%
!! AGTGGTGATGGAAAAATTAT+AGG - chr1.4:51854758-51854777 MS.gene47447:intron 30.0%
!!! TTTTTTGTCAGCTAGTTTAG+TGG + chr1.4:51855570-51855589 None:intergenic 30.0%
ACATGGCTGCAATTGTATAA+TGG + chr1.4:51855768-51855787 None:intergenic 35.0%
ACTAATCAGATTCATACCTG+AGG + chr1.4:51854213-51854232 None:intergenic 35.0%
AGTGTAGATACTCTATCTAC+TGG - chr1.4:51854246-51854265 MS.gene47447:CDS 35.0%
ATAGAACCATAAACCCACAT+TGG + chr1.4:51855955-51855974 None:intergenic 35.0%
ATAGACTAACACCTGAATGT+TGG - chr1.4:51856223-51856242 MS.gene47447:CDS 35.0%
ATCTACTGGAAAAATTACGG+TGG - chr1.4:51854260-51854279 MS.gene47447:CDS 35.0%
ATTTCCACTAAGACCAATGT+GGG - chr1.4:51855939-51855958 MS.gene47447:intron 35.0%
CACATTATATGTGGAGAAGT+AGG + chr1.4:51855536-51855555 None:intergenic 35.0%
GCTGCAATTGTATAATGGTA+TGG + chr1.4:51855763-51855782 None:intergenic 35.0%
GGATTCACACACATTATATG+TGG + chr1.4:51855545-51855564 None:intergenic 35.0%
GTCTGATTCTTACGGAATAT+AGG - chr1.4:51855347-51855366 MS.gene47447:intron 35.0%
TACACAAACTTCAAAGCTAG+TGG - chr1.4:51856051-51856070 MS.gene47447:CDS 35.0%
TAGACTAACACCTGAATGTT+GGG - chr1.4:51856224-51856243 MS.gene47447:CDS 35.0%
TCTACTTCATCATGAAATCC+TGG + chr1.4:51854671-51854690 None:intergenic 35.0%
TGTACTTTGCAACAAAAGGT+TGG + chr1.4:51856035-51856054 None:intergenic 35.0%
TTCACGAAATCAAATTGTGC+CGG + chr1.4:51854339-51854358 None:intergenic 35.0%
TTTGTGCATGTTTGAATTCG+CGG + chr1.4:51854367-51854386 None:intergenic 35.0%
! AACAACATTGGTCTATGAGT+TGG - chr1.4:51856148-51856167 MS.gene47447:CDS 35.0%
! AACATTCAGGTGTTAGTCTA+TGG + chr1.4:51856224-51856243 None:intergenic 35.0%
! ACAACATTGGTCTATGAGTT+GGG - chr1.4:51856149-51856168 MS.gene47447:CDS 35.0%
! AGGTCTATGAGCTTTTACAA+TGG - chr1.4:51854116-51854135 MS.gene47447:CDS 35.0%
! GAATTTCTAGGGACAACATT+TGG - chr1.4:51854695-51854714 MS.gene47447:intron 35.0%
! GTGGGTTTATGGTTCTATTT+GGG - chr1.4:51855957-51855976 MS.gene47447:intron 35.0%
! TATTTGGGATGCTTCATCAT+GGG - chr1.4:51855972-51855991 MS.gene47447:intron 35.0%
! TGCTATAGTTTGGAATCCTA+AGG - chr1.4:51854835-51854854 MS.gene47447:intron 35.0%
! TGTGGGTTTATGGTTCTATT+TGG - chr1.4:51855956-51855975 MS.gene47447:intron 35.0%
AAAGCTCATAGACCTGACTT+TGG + chr1.4:51854111-51854130 None:intergenic 40.0%
AATGTTGCATCACTCTTCCT+TGG + chr1.4:51855922-51855941 None:intergenic 40.0%
AGACTAACACCTGAATGTTG+GGG - chr1.4:51856225-51856244 MS.gene47447:CDS 40.0%
ATGTTGCATCACTCTTCCTT+GGG + chr1.4:51855921-51855940 None:intergenic 40.0%
CATTTCCACTAAGACCAATG+TGG - chr1.4:51855938-51855957 MS.gene47447:intron 40.0%
CTAAGACCAATGTGGGTTTA+TGG - chr1.4:51855946-51855965 MS.gene47447:intron 40.0%
CTCTTATAGTTGAGATCGGA+GGG + chr1.4:51854918-51854937 None:intergenic 40.0%
GCTTCGATTAAACTTCATCC+TGG - chr1.4:51854479-51854498 MS.gene47447:intron 40.0%
GGTTGACAAGACAACAACAT+TGG - chr1.4:51856136-51856155 MS.gene47447:CDS 40.0%
TATATAAGAGGGAGTGGTGA+TGG - chr1.4:51854746-51854765 MS.gene47447:intron 40.0%
TCTCTTATAGTTGAGATCGG+AGG + chr1.4:51854919-51854938 None:intergenic 40.0%
TTCCATGTTGATCCATGCTT+TGG + chr1.4:51854169-51854188 None:intergenic 40.0%
! CTATTTGGGATGCTTCATCA+TGG - chr1.4:51855971-51855990 MS.gene47447:intron 40.0%
! TAGTGGATGATATGCCCATA+AGG - chr1.4:51855890-51855909 MS.gene47447:intron 40.0%
!! ATAGACCTGACTTTGGAACT+TGG + chr1.4:51854104-51854123 None:intergenic 40.0%
!! ATTTGGCTTGATAGAACCTC+AGG - chr1.4:51854194-51854213 MS.gene47447:CDS 40.0%
!! GTTAGTCTATGGTCCCTTTT+AGG + chr1.4:51856213-51856232 None:intergenic 40.0%
!! TAAACCCACATTGGTCTTAG+TGG + chr1.4:51855946-51855965 None:intergenic 40.0%
CACCAAAGCATGGATCAACA+TGG - chr1.4:51854164-51854183 MS.gene47447:CDS 45.0%
CCATTTCGATCGGGATACTT+TGG - chr1.4:51854455-51854474 MS.gene47447:intron 45.0%
CTGCTTCAAGCAATGCCTAT+TGG - chr1.4:51854057-51854076 MS.gene47447:CDS 45.0%
GTAGGGAATCCAGATTCGAA+AGG + chr1.4:51855518-51855537 None:intergenic 45.0%
GTCGAATCGTCCATTTCGAT+CGG - chr1.4:51854445-51854464 MS.gene47447:intron 45.0%
TCATCATGGGCAACAGAAGA+TGG - chr1.4:51855985-51856004 MS.gene47447:intron 45.0%
TCGAATCGTCCATTTCGATC+GGG - chr1.4:51854446-51854465 MS.gene47447:intron 45.0%
TCTAACAATGAAGCGCATCC+AGG - chr1.4:51854650-51854669 MS.gene47447:intron 45.0%
TGGATGATATGCCCATAAGG+AGG - chr1.4:51855893-51855912 MS.gene47447:intron 45.0%
TTCCTTGGGTACCTCCTTAT+GGG + chr1.4:51855907-51855926 None:intergenic 45.0%
TTGGGTTCAAAGACACCACA+TGG - chr1.4:51856167-51856186 MS.gene47447:CDS 45.0%
TTGTTGTCTTGTCAACCCAC+CGG + chr1.4:51856133-51856152 None:intergenic 45.0%
! CCAAAGTATCCCGATCGAAA+TGG + chr1.4:51854458-51854477 None:intergenic 45.0%
! TTGTCAGCTAGTTTAGTGGC+TGG + chr1.4:51855566-51855585 None:intergenic 45.0%
!! TGTGCCGGCCATTGTAATTT+TGG + chr1.4:51854324-51854343 None:intergenic 45.0%
ACTGGCCAAGTTCCAAAGTC+AGG - chr1.4:51854096-51854115 MS.gene47447:CDS 50.0%
ATAACTCCTGCAGTGTAGCC+AGG + chr1.4:51854500-51854519 None:intergenic 50.0%
CTTCCTTGGGTACCTCCTTA+TGG + chr1.4:51855908-51855927 None:intergenic 50.0%
GTTGCACAGCCTATGCATCA+CGG - chr1.4:51856073-51856092 MS.gene47447:CDS 50.0%
TAGTTGAGATCGGAGGGAGT+AGG + chr1.4:51854912-51854931 None:intergenic 50.0%
TGGAACTTGGCCAGTAACCA+GGG + chr1.4:51854091-51854110 None:intergenic 50.0%
TTGGAACTTGGCCAGTAACC+AGG + chr1.4:51854092-51854111 None:intergenic 50.0%
!! TGATCCATGCTTTGGTGCTG+AGG + chr1.4:51854161-51854180 None:intergenic 50.0%
ACGACACCACCGTGATGCAT+AGG + chr1.4:51856085-51856104 None:intergenic 55.0%
CTTCATCCTGGCTACACTGC+AGG - chr1.4:51854491-51854510 MS.gene47447:intron 55.0%
GGAACTTGGCCAGTAACCAG+GGG + chr1.4:51854090-51854109 None:intergenic 55.0%
!! TGCCCATAAGGAGGTACCCA+AGG - chr1.4:51855902-51855921 MS.gene47447:intron 55.0%
!! TTTGGTGCTGAGGACCCCAA+AGG + chr1.4:51854151-51854170 None:intergenic 55.0%
ACCAGGGGAAGGTGGCCAAT+AGG + chr1.4:51854075-51854094 None:intergenic 60.0%
CTTGGCCAGTAACCAGGGGA+AGG + chr1.4:51854086-51854105 None:intergenic 60.0%
GCACAGCCTATGCATCACGG+TGG - chr1.4:51856076-51856095 MS.gene47447:CDS 60.0%
GGGTCCTCAGCACCAAAGCA+TGG - chr1.4:51854154-51854173 MS.gene47447:CDS 60.0%
GTCAACCCACCGGACCGATA+CGG + chr1.4:51856123-51856142 None:intergenic 60.0%
!! TGCATCACGGTGGTGTCGTC+CGG - chr1.4:51856086-51856105 MS.gene47447:CDS 60.0%
GCCTATTGGCCACCTTCCCC+TGG - chr1.4:51854071-51854090 MS.gene47447:CDS 65.0%
GGCCACCTTCCCCTGGTTAC+TGG - chr1.4:51854078-51854097 MS.gene47447:CDS 65.0%
GGCCAGTAACCAGGGGAAGG+TGG + chr1.4:51854083-51854102 None:intergenic 65.0%
! ACGGCCTCGCCGTATCGGTC+CGG - chr1.4:51856111-51856130 MS.gene47447:CDS 70.0%
! CCTCGCCGTATCGGTCCGGT+GGG - chr1.4:51856115-51856134 MS.gene47447:CDS 70.0%
!! ACGGTGGTGTCGTCCGGCCA+CGG - chr1.4:51856092-51856111 MS.gene47447:CDS 70.0%
CCCACCGGACCGATACGGCG+AGG + chr1.4:51856118-51856137 None:intergenic 75.0%
CGATACGGCGAGGCCGTGGC+CGG + chr1.4:51856108-51856127 None:intergenic 75.0%
CGGCCACGGCCTCGCCGTAT+CGG - chr1.4:51856106-51856125 MS.gene47447:CDS 75.0%
GGACCGATACGGCGAGGCCG+TGG + chr1.4:51856112-51856131 None:intergenic 75.0%
! GCCTCGCCGTATCGGTCCGG+TGG - chr1.4:51856114-51856133 MS.gene47447:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 51854011 51856251 51854011 ID=MS.gene47447
chr1.4 mRNA 51854011 51856251 51854011 ID=MS.gene47447.t1;Parent=MS.gene47447
chr1.4 exon 51856047 51856251 51856047 ID=MS.gene47447.t1.exon1;Parent=MS.gene47447.t1
chr1.4 CDS 51856047 51856251 51856047 ID=cds.MS.gene47447.t1;Parent=MS.gene47447.t1
chr1.4 exon 51855730 51855830 51855730 ID=MS.gene47447.t1.exon2;Parent=MS.gene47447.t1
chr1.4 CDS 51855730 51855830 51855730 ID=cds.MS.gene47447.t1;Parent=MS.gene47447.t1
chr1.4 exon 51855398 51855615 51855398 ID=MS.gene47447.t1.exon3;Parent=MS.gene47447.t1
chr1.4 CDS 51855398 51855615 51855398 ID=cds.MS.gene47447.t1;Parent=MS.gene47447.t1
chr1.4 exon 51854011 51854377 51854011 ID=MS.gene47447.t1.exon4;Parent=MS.gene47447.t1
chr1.4 CDS 51854011 51854377 51854011 ID=cds.MS.gene47447.t1;Parent=MS.gene47447.t1
Gene Sequence

>MS.gene47447

ATGGCTTTTCCTTTCATATTTCTCTTTGCTAGTTTATTCTTTATTGCTGCTTCAAGCAATGCCTATTGGCCACCTTCCCCTGGTTACTGGCCAAGTTCCAAAGTCAGGTCTATGAGCTTTTACAATGGTTATAGAAACCTTTGGGGTCCTCAGCACCAAAGCATGGATCAACATGGAACAACAATTTGGCTTGATAGAACCTCAGGTATGAATCTGATTAGTTTCGATTCACGTTAGTGTAGATACTCTATCTACTGGAAAAATTACGGTGGTACCATGATTTTGCTGAAACTTTAAAGTGTAGTTATTTCCAAAATTACAATGGCCGGCACAATTTGATTTCGTGAAACTTACCGCGAATTCAAACATGCACAAAATATATCATTGACATGTAACCTACATTTTTCTTTCTAATTATTAGGAAGTGGATTCAAGTCGAATCGTCCATTTCGATCGGGATACTTTGGCGCTTCGATTAAACTTCATCCTGGCTACACTGCAGGAGTTATAACAGCTTTTTATGTAAGATTAAGATATGATCATGATTAAAAAAATGAAGTTGATTAATAGAAGGTTAAACATTTTTTTTTAATTTGAGTGTGTGATAAATTTTACTAATGAGTTGATCAATTGCAGCTTTCTAACAATGAAGCGCATCCAGGATTTCATGATGAAGTAGACATTGAATTTCTAGGGACAACATTTGGAAAGCCTTATACTTTGCAGACAAATGTTTATATAAGAGGGAGTGGTGATGGAAAAATTATAGGCAGAGAAATGAAATTTCATCTGTGGTTTGATCCTACCAAAAAATTTCATCACTATGCTATAGTTTGGAATCCTAAGGAATTAATGTAAGTCACTATCAATTTTTACTCTATATTAATACTCTTTCGATCCTACTCCCTCCGATCTCAACTATAAGAGACATTTCACTTAATAAATGGTATTGTGTCTAGTTTCTAATATTGATTAGATACATAATTTATTGAATGAATTAAAAAAAATTAATTTTTTCTTATAATTGAGACCGACATAGTATAATAATCGTCACAGAGAAAATTTATCCTTTAAAGAGTGTCACATTTAAGATATTATTTTTCACTTTTGTATTTATAATAATTGAAATAACCACAAGTTTATAAGGATAGTTTATTAAAATTACCATTTTATTTCATTTATTTATCATCGGTTAGACTTTATTTTTGTGCTAGTTCAAAAAAGTAGAGCATATAGGGTAGCCAATATTTCTTTTTCTGCTGAAACATAATAGGGTAGATGATAACAAAAAAATGCAATAATGTAACTTGGGTCTTTGTCTGTGTCATATTGATTTGTCTGATTCTTACGGAATATAGGTAATACAACAGTGTATGCACGAATGATTGACATTTTGTAAAATGAAATGGAAGCAAACCAATAATACCATTTTCTTTGAAAAAAATATATCATTTTCTTTGAAATTAAAGAATGTGAGAATAGAAGTATAATCTTTTTTATTATTCCTTTCGAATCTGGATTCCCTACTTCTCCACATATAATGTGTGTGAATCCAGCCACTAAACTAGCTGACAAAAAAAAAAAAAGTAGAGAGTTGTCTTACTAAATTTTAATCATAACAATTTTTTTTTTTTATTTCAATCTAATGGTCATGATATTGAAGGGGAACCTTACTAATTATAAATGGCACTAATAACAATCTGATCTACATGTGTTTTTTTTATACCTTATTATATATAATTAGTTTCACCATACCATTATACAATTGCAGCCATGTGAAAGTGATTTTTTCTTTGGTACAGTGAAGTGATTTTTATTAACTTGTTTTTGTAATATATATTCTTTAACTTACATTATAATTAAACAATGTACAGATTCTTAGTGGATGATATGCCCATAAGGAGGTACCCAAGGAAGAGTGATGCAACATTTCCACTAAGACCAATGTGGGTTTATGGTTCTATTTGGGATGCTTCATCATGGGCAACAGAAGATGGAAAATACAAAGCTGATTACAAATACCAACCTTTTGTTGCAAAGTACACAAACTTCAAAGCTAGTGGTTGCACAGCCTATGCATCACGGTGGTGTCGTCCGGCCACGGCCTCGCCGTATCGGTCCGGTGGGTTGACAAGACAACAACATTGGTCTATGAGTTGGGTTCAAAGACACCACATGGTTTATAACTATTGTCAAGATCCTAAAAGGGACCATAGACTAACACCTGAATGTTGGGGTTAA
Protein sequence

>MS.gene47447.t1

MAFPFIFLFASLFFIAASSNAYWPPSPGYWPSSKVRSMSFYNGYRNLWGPQHQSMDQHGTTIWLDRTSGSGFKSNRPFRSGYFGASIKLHPGYTAGVITAFYLSNNEAHPGFHDEVDIEFLGTTFGKPYTLQTNVYIRGSGDGKIIGREMKFHLWFDPTKKFHHYAIVWNPKELIFLVDDMPIRRYPRKSDATFPLRPMWVYGSIWDASSWATEDGKYKADYKYQPFVAKYTNFKASGCTAYASRWCRPATASPYRSGGLTRQQHWSMSWVQRHHMVYNYCQDPKRDHRLTPECWG