Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47472.t1 | XP_024629542.1 | 97.8 | 322 | 7 | 0 | 1 | 322 | 1 | 322 | 1.40E-177 | 632.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47472.t1 | Q9NX74 | 51.9 | 314 | 144 | 3 | 3 | 312 | 10 | 320 | 1.6e-81 | 304.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47472.t1 | A0A396GKI1 | 97.8 | 322 | 7 | 0 | 1 | 322 | 1 | 322 | 1.0e-177 | 632.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene47472 | 0.828226 | 1.01E-54 | -1.69E-46 |
| MS.gene050953 | MS.gene47472 | 0.812475 | 4.16E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene47472 | 0.823743 | 1.18E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene47472 | 0.804727 | 1.88E-49 | -1.69E-46 |
| MS.gene052389 | MS.gene47472 | 0.85834 | 8.81E-63 | -1.69E-46 |
| MS.gene054405 | MS.gene47472 | 0.802463 | 5.54E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene47472 | 0.814043 | 1.88E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene47472 | 0.801276 | 9.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47472.t1 | MTR_8g067420 | 97.690 | 303 | 7 | 0 | 1 | 303 | 1 | 303 | 0.0 | 615 |
| MS.gene47472.t1 | MTR_8g067420 | 97.333 | 225 | 6 | 0 | 1 | 225 | 1 | 225 | 8.36e-163 | 454 |
| MS.gene47472.t1 | MTR_8g067420 | 97.333 | 225 | 6 | 0 | 1 | 225 | 1 | 225 | 1.47e-162 | 453 |
| MS.gene47472.t1 | MTR_4g094905 | 34.979 | 243 | 138 | 4 | 4 | 243 | 39 | 264 | 2.31e-34 | 129 |
| MS.gene47472.t1 | MTR_8g006745 | 97.674 | 43 | 1 | 0 | 183 | 225 | 34 | 76 | 1.43e-22 | 91.7 |
| MS.gene47472.t1 | MTR_3g117580 | 29.200 | 250 | 154 | 7 | 1 | 243 | 334 | 567 | 3.36e-22 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47472.t1 | AT3G49640 | 69.716 | 317 | 96 | 0 | 1 | 317 | 1 | 317 | 6.92e-172 | 480 |
| MS.gene47472.t1 | AT3G49640 | 71.617 | 303 | 86 | 0 | 1 | 303 | 1 | 303 | 8.22e-169 | 471 |
| MS.gene47472.t1 | AT3G49640 | 68.561 | 264 | 83 | 0 | 54 | 317 | 15 | 278 | 4.07e-138 | 393 |
| MS.gene47472.t1 | AT3G49640 | 70.800 | 250 | 73 | 0 | 54 | 303 | 15 | 264 | 1.09e-134 | 383 |
| MS.gene47472.t1 | AT3G49640 | 77.391 | 230 | 52 | 0 | 1 | 230 | 1 | 230 | 5.51e-133 | 378 |
| MS.gene47472.t1 | AT5G67220 | 34.568 | 243 | 139 | 4 | 4 | 243 | 87 | 312 | 5.95e-34 | 129 |
| MS.gene47472.t1 | AT4G38890 | 28.990 | 307 | 182 | 12 | 1 | 288 | 334 | 623 | 2.73e-22 | 97.8 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCACTTTGCTGGATCTC+TGG | 0.214344 | 8.4:-35363221 | None:intergenic |
| ACATCACCATTTGCTATTAC+TGG | 0.260014 | 8.4:-35363277 | None:intergenic |
| GATAGAGTTGTGTTTCAAAT+AGG | 0.280513 | 8.4:+35360382 | MS.gene47472:CDS |
| GAAGCACCAAACATACATTA+AGG | 0.283111 | 8.4:+35365299 | MS.gene47472:CDS |
| TTGACCACGGCCCTCCATTC+AGG | 0.284520 | 8.4:-35365355 | None:intergenic |
| CGCTATCTCTGCTGTGGTCT+CGG | 0.290167 | 8.4:-35365380 | None:intergenic |
| CATATCCCTTAATGTATGTT+TGG | 0.332608 | 8.4:-35365305 | None:intergenic |
| GCAATTCCACTCCACTTTGC+TGG | 0.334192 | 8.4:-35363229 | None:intergenic |
| CTCCACTTTGCTGGATCTCT+GGG | 0.351036 | 8.4:-35363220 | None:intergenic |
| AGATTACTAGCTGCTGAATA+TGG | 0.352000 | 8.4:+35358566 | MS.gene47472:CDS |
| AGTCGAATCTTACATGTTAC+AGG | 0.358585 | 8.4:-35362255 | None:intergenic |
| TTTACAGATATGTCTTAGAT+AGG | 0.377928 | 8.4:+35363197 | MS.gene47472:intron |
| CGATTTCCAACGCATTAAAT+CGG | 0.395319 | 8.4:+35363312 | MS.gene47472:CDS |
| ACACTAACTGAGCAGCAGTT+AGG | 0.403336 | 8.4:-35360426 | None:intergenic |
| TTCAGATCTTAACAACATTA+CGG | 0.406436 | 8.4:+35362217 | MS.gene47472:intron |
| TCGTAAGAAACTTCGCCTTC+AGG | 0.407736 | 8.4:-35365089 | None:intergenic |
| ATTTCTTGGTCAGAACATCT+TGG | 0.411985 | 8.4:+35360295 | MS.gene47472:intron |
| ATCACTGATAAGCTCTGGTT+TGG | 0.427978 | 8.4:-35361943 | None:intergenic |
| GCCAGGCGAATTGAGAAAAC+TGG | 0.435894 | 8.4:+35362309 | MS.gene47472:CDS |
| CACTAACTGAGCAGCAGTTA+GGG | 0.436616 | 8.4:-35360425 | None:intergenic |
| CCTTCAGGTGAAAATATTGA+AGG | 0.443848 | 8.4:-35365074 | None:intergenic |
| ATGATACAGTGGAGCTTGCC+AGG | 0.449136 | 8.4:+35362292 | MS.gene47472:CDS |
| CCGCTCAAAGAAAATGCCTT+GGG | 0.450971 | 8.4:-35361897 | None:intergenic |
| GATGTTTCAGGGAACTCTAC+GGG | 0.464810 | 8.4:+35367434 | MS.gene47472:intron |
| AATTTGGAGATTACCTTCCA+TGG | 0.468028 | 8.4:-35362350 | None:intergenic |
| GGAAGAGTGTACCGTTGATT+CGG | 0.476162 | 8.4:-35358646 | None:intergenic |
| CAACGCATTAAATCGGCTAC+AGG | 0.483389 | 8.4:+35363319 | MS.gene47472:CDS |
| TTGATTCCAGTGCATCTTGT+GGG | 0.490842 | 8.4:+35365264 | MS.gene47472:intron |
| TGACCACGGCCCTCCATTCA+GGG | 0.494351 | 8.4:-35365354 | None:intergenic |
| AAGCACCAAACATACATTAA+GGG | 0.495838 | 8.4:+35365300 | MS.gene47472:CDS |
| CCTCTCTTGAAGTCCCTGAA+TGG | 0.501215 | 8.4:+35365341 | MS.gene47472:CDS |
| GTTGATGCTCTTGCAGTCCA+TGG | 0.506359 | 8.4:+35362333 | MS.gene47472:CDS |
| TCTATTCCAGTAATAGCAAA+TGG | 0.512803 | 8.4:+35363271 | MS.gene47472:CDS |
| ACCGCTCAAAGAAAATGCCT+TGG | 0.513927 | 8.4:-35361898 | None:intergenic |
| TGGCTGCAAGAGGGGCACTT+TGG | 0.516720 | 8.4:+35365048 | MS.gene47472:CDS |
| CAACATTATCTATACTCTGT+AGG | 0.518601 | 8.4:-35367491 | None:intergenic |
| TGATGTTTCAGGGAACTCTA+CGG | 0.527225 | 8.4:+35367433 | MS.gene47472:intron |
| GTCGGTGATGGCTGCAAGAG+GGG | 0.530783 | 8.4:+35365040 | MS.gene47472:CDS |
| AAACTCACAACGCGAACCAT+AGG | 0.535701 | 8.4:-35358427 | None:intergenic |
| ATAAGGTGCTTCGTCGGTGA+TGG | 0.537137 | 8.4:+35365028 | MS.gene47472:intron |
| CGTCGGTGATGGCTGCAAGA+GGG | 0.537292 | 8.4:+35365039 | MS.gene47472:CDS |
| CTTACATCACTGATAAGCTC+TGG | 0.538803 | 8.4:-35361948 | None:intergenic |
| CTCTTGAAGTCCCTGAATGG+AGG | 0.541254 | 8.4:+35365344 | MS.gene47472:CDS |
| TATGGTGCAGATATCACTTA+CGG | 0.544347 | 8.4:+35358584 | MS.gene47472:CDS |
| TCGTCGGTGATGGCTGCAAG+AGG | 0.548580 | 8.4:+35365038 | MS.gene47472:CDS |
| AGCAGCAGTTAGGGCCCTCA+CGG | 0.548803 | 8.4:-35360416 | None:intergenic |
| AAATGCGAACGCCGAATCAA+CGG | 0.555297 | 8.4:+35358635 | MS.gene47472:CDS |
| TTTGATTCCAGTGCATCTTG+TGG | 0.562054 | 8.4:+35365263 | MS.gene47472:intron |
| CTGTAGCCGATTTAATGCGT+TGG | 0.566986 | 8.4:-35363318 | None:intergenic |
| TCAGCAGCTAGTAATCTAAG+TGG | 0.570303 | 8.4:-35358560 | None:intergenic |
| CCCTTTCTCCACAAAATCAG+TGG | 0.575858 | 8.4:-35360320 | None:intergenic |
| TCTTGAAGTCCCTGAATGGA+GGG | 0.575937 | 8.4:+35365345 | MS.gene47472:CDS |
| ATGCTCTTGCAGTCCATGGA+AGG | 0.599355 | 8.4:+35362337 | MS.gene47472:CDS |
| AGTCCCTGAATGGAGGGCCG+TGG | 0.602370 | 8.4:+35365351 | MS.gene47472:CDS |
| TTTGGTATAAGGTGCTTCGT+CGG | 0.606608 | 8.4:+35365022 | MS.gene47472:intron |
| TGCTGCAATAGATATTAACA+TGG | 0.611209 | 8.4:+35361869 | MS.gene47472:CDS |
| AGGCACATCTGATGCCGTGA+GGG | 0.612052 | 8.4:+35360402 | MS.gene47472:CDS |
| AACTTACGCTATCTCTGCTG+TGG | 0.623296 | 8.4:-35365386 | None:intergenic |
| GGCCCAGAGATCCAGCAAAG+TGG | 0.629436 | 8.4:+35363218 | MS.gene47472:CDS |
| AGAGATCCAGCAAAGTGGAG+TGG | 0.635012 | 8.4:+35363223 | MS.gene47472:CDS |
| TATTAACATGGGATGCCCCA+AGG | 0.638952 | 8.4:+35361881 | MS.gene47472:CDS |
| TGTGGTCTCGGATTTGACCA+CGG | 0.644228 | 8.4:-35365368 | None:intergenic |
| CCATTCAGGGACTTCAAGAG+AGG | 0.645488 | 8.4:-35365341 | None:intergenic |
| AAAATTACCGCATGATACAG+TGG | 0.653849 | 8.4:+35362281 | MS.gene47472:CDS |
| CGCTCAAAGAAAATGCCTTG+GGG | 0.655009 | 8.4:-35361896 | None:intergenic |
| GAATCTTACATGTTACAGGT+AGG | 0.659712 | 8.4:-35362251 | None:intergenic |
| TGAAGGCGAAGTTTCTTACG+AGG | 0.667759 | 8.4:+35365091 | MS.gene47472:CDS |
| ATGTTTCAGGGAACTCTACG+GGG | 0.668030 | 8.4:+35367435 | MS.gene47472:intron |
| GCAAGCTCCACTGTATCATG+CGG | 0.672086 | 8.4:-35362288 | None:intergenic |
| AGATAGTGCTGACACAACAT+CGG | 0.679463 | 8.4:-35363251 | None:intergenic |
| TAGGCACATCTGATGCCGTG+AGG | 0.684402 | 8.4:+35360401 | MS.gene47472:CDS |
| AGATCTTAACAACATTACGG+AGG | 0.701161 | 8.4:+35362220 | MS.gene47472:intron |
| GCTGCAATAGATATTAACAT+GGG | 0.721113 | 8.4:+35361870 | MS.gene47472:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 35358397 | 35367504 | 35358397 | ID=MS.gene47472 |
| chr8.4 | mRNA | 35358397 | 35367504 | 35358397 | ID=MS.gene47472.t1;Parent=MS.gene47472 |
| chr8.4 | exon | 35358397 | 35358441 | 35358397 | ID=MS.gene47472.t1.exon1;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35358397 | 35358441 | 35358397 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35358551 | 35358656 | 35358551 | ID=MS.gene47472.t1.exon2;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35358551 | 35358656 | 35358551 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35360308 | 35360446 | 35360308 | ID=MS.gene47472.t1.exon3;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35360308 | 35360446 | 35360308 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35361857 | 35361965 | 35361857 | ID=MS.gene47472.t1.exon4;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35361857 | 35361965 | 35361857 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35362222 | 35362358 | 35362222 | ID=MS.gene47472.t1.exon5;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35362222 | 35362358 | 35362222 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35363204 | 35363340 | 35363204 | ID=MS.gene47472.t1.exon6;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35363204 | 35363340 | 35363204 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35365033 | 35365139 | 35365033 | ID=MS.gene47472.t1.exon7;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35365033 | 35365139 | 35365033 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35365274 | 35365401 | 35365274 | ID=MS.gene47472.t1.exon8;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35365274 | 35365401 | 35365274 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| chr8.4 | exon | 35367444 | 35367504 | 35367444 | ID=MS.gene47472.t1.exon9;Parent=MS.gene47472.t1 |
| chr8.4 | CDS | 35367444 | 35367504 | 35367444 | ID=cds.MS.gene47472.t1;Parent=MS.gene47472.t1 |
| Gene Sequence |
| Protein sequence |
