Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054610.t1 | XP_003602984.1 | 69.3 | 374 | 115 | 0 | 1 | 374 | 16 | 389 | 6.50E-142 | 513.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054610.t1 | Q9S7T8 | 48.7 | 378 | 186 | 4 | 1 | 372 | 15 | 390 | 2.5e-97 | 357.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054610.t1 | G7J5R9 | 69.3 | 374 | 115 | 0 | 1 | 374 | 16 | 389 | 4.7e-142 | 513.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049669 | MS.gene054610 | 0.80082 | 1.20E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene054610 | 0.800761 | 1.24E-48 | -1.69E-46 |
| MS.gene050228 | MS.gene054610 | 0.802385 | 5.75E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene054610 | 0.812593 | 3.92E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene054610 | 0.802293 | 6.01E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene054610 | 0.803354 | 3.63E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene054610 | 0.823343 | 1.46E-53 | -1.69E-46 |
| MS.gene054468 | MS.gene054610 | 0.808041 | 3.76E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene055356 | 0.824903 | 6.27E-54 | -1.69E-46 |
| MS.gene054610 | MS.gene055701 | 0.809829 | 1.56E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene061040 | -0.808116 | 3.63E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene061114 | -0.804679 | 1.92E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene061333 | 0.806769 | 7.00E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene061343 | 0.80898 | 2.37E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene065923 | 0.8056 | 1.23E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene066206 | 0.822078 | 2.87E-53 | -1.69E-46 |
| MS.gene054610 | MS.gene06698 | 0.806588 | 7.65E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene069554 | 0.801817 | 7.53E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene071059 | 0.807253 | 5.53E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene072397 | -0.805964 | 1.04E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene08778 | 0.812121 | 4.97E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene26728 | 0.800061 | 1.72E-48 | -1.69E-46 |
| MS.gene054610 | MS.gene29086 | 0.819285 | 1.26E-52 | -1.69E-46 |
| MS.gene054610 | MS.gene31397 | 0.812852 | 3.44E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene32231 | 0.826228 | 3.04E-54 | -1.69E-46 |
| MS.gene054610 | MS.gene32717 | 0.803059 | 4.18E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene32874 | 0.815661 | 8.25E-52 | -1.69E-46 |
| MS.gene054610 | MS.gene35408 | 0.812968 | 3.25E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene41424 | 0.809541 | 1.80E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene43040 | 0.801799 | 7.59E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene44461 | 0.814809 | 1.27E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene44474 | -0.803232 | 3.85E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene45090 | 0.800423 | 1.45E-48 | -1.69E-46 |
| MS.gene054610 | MS.gene46126 | 0.808559 | 2.92E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene47472 | 0.814043 | 1.88E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene52059 | 0.83383 | 4.26E-56 | -1.69E-46 |
| MS.gene054610 | MS.gene52061 | 0.80723 | 5.59E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene59840 | 0.808688 | 2.74E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene61139 | 0.821322 | 4.29E-53 | -1.69E-46 |
| MS.gene054610 | MS.gene61547 | -0.81004 | 1.40E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene63412 | 0.812326 | 4.48E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene64182 | 0.804839 | 1.78E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene68333 | 0.804756 | 1.85E-49 | -1.69E-46 |
| MS.gene054610 | MS.gene69794 | 0.825266 | 5.15E-54 | -1.69E-46 |
| MS.gene054610 | MS.gene70022 | 0.818428 | 1.97E-52 | -1.69E-46 |
| MS.gene054610 | MS.gene71104 | 0.81174 | 6.02E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene77781 | 0.809926 | 1.49E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene80383 | 0.808341 | 3.25E-50 | -1.69E-46 |
| MS.gene054610 | MS.gene83831 | 0.800081 | 1.70E-48 | -1.69E-46 |
| MS.gene054610 | MS.gene84326 | 0.811971 | 5.36E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene84931 | 0.813622 | 2.33E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene91005 | 0.833732 | 4.50E-56 | -1.69E-46 |
| MS.gene054610 | MS.gene91809 | 0.814726 | 1.33E-51 | -1.69E-46 |
| MS.gene054610 | MS.gene93041 | 0.801014 | 1.10E-48 | -1.69E-46 |
| MS.gene054610 | MS.gene99802 | 0.809582 | 1.76E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054610.t1 | MTR_3g101130 | 69.251 | 374 | 115 | 0 | 1 | 374 | 16 | 389 | 0.0 | 523 |
| MS.gene054610.t1 | MTR_7g451890 | 64.644 | 379 | 129 | 2 | 1 | 374 | 24 | 402 | 1.72e-173 | 489 |
| MS.gene054610.t1 | MTR_7g050750 | 64.908 | 379 | 128 | 2 | 1 | 374 | 18 | 396 | 8.45e-173 | 487 |
| MS.gene054610.t1 | MTR_3g101180 | 63.708 | 383 | 128 | 3 | 1 | 372 | 16 | 398 | 1.32e-172 | 487 |
| MS.gene054610.t1 | MTR_7g047780 | 64.644 | 379 | 129 | 2 | 1 | 374 | 24 | 402 | 4.47e-172 | 485 |
| MS.gene054610.t1 | MTR_8g461160 | 64.987 | 377 | 127 | 2 | 1 | 372 | 16 | 392 | 2.16e-171 | 483 |
| MS.gene054610.t1 | MTR_7g050810 | 62.533 | 379 | 137 | 2 | 1 | 374 | 26 | 404 | 7.24e-167 | 472 |
| MS.gene054610.t1 | MTR_3g101010 | 64.550 | 378 | 127 | 3 | 1 | 374 | 15 | 389 | 2.51e-166 | 470 |
| MS.gene054610.t1 | MTR_8g027395 | 63.588 | 379 | 125 | 5 | 1 | 374 | 27 | 397 | 5.06e-165 | 467 |
| MS.gene054610.t1 | MTR_3g018740 | 63.228 | 378 | 127 | 4 | 1 | 372 | 15 | 386 | 9.34e-162 | 459 |
| MS.gene054610.t1 | MTR_4g045707 | 62.797 | 379 | 130 | 4 | 1 | 374 | 16 | 388 | 2.85e-161 | 457 |
| MS.gene054610.t1 | MTR_3g101030 | 58.291 | 398 | 135 | 4 | 1 | 374 | 39 | 429 | 2.31e-157 | 449 |
| MS.gene054610.t1 | MTR_8g027420 | 60.847 | 378 | 126 | 5 | 1 | 374 | 16 | 375 | 5.46e-153 | 436 |
| MS.gene054610.t1 | MTR_3g101200 | 62.943 | 367 | 114 | 3 | 7 | 372 | 45 | 390 | 2.72e-152 | 435 |
| MS.gene054610.t1 | MTR_4g088525 | 59.890 | 364 | 135 | 3 | 15 | 373 | 11 | 368 | 5.56e-148 | 423 |
| MS.gene054610.t1 | MTR_3g101050 | 60.733 | 382 | 118 | 9 | 1 | 373 | 39 | 397 | 1.62e-146 | 421 |
| MS.gene054610.t1 | MTR_3g048000 | 56.992 | 379 | 131 | 6 | 1 | 372 | 37 | 390 | 5.93e-141 | 406 |
| MS.gene054610.t1 | MTR_2g046030 | 56.053 | 380 | 151 | 5 | 1 | 372 | 15 | 386 | 1.66e-140 | 405 |
| MS.gene054610.t1 | MTR_2g045980 | 56.053 | 380 | 151 | 5 | 1 | 372 | 15 | 386 | 2.63e-140 | 404 |
| MS.gene054610.t1 | MTR_4g088535 | 57.258 | 372 | 132 | 5 | 1 | 368 | 17 | 365 | 1.28e-135 | 392 |
| MS.gene054610.t1 | MTR_3g015620 | 57.520 | 379 | 128 | 6 | 1 | 372 | 26 | 378 | 1.69e-134 | 389 |
| MS.gene054610.t1 | MTR_3g102920 | 54.474 | 380 | 126 | 7 | 1 | 372 | 68 | 408 | 2.28e-126 | 370 |
| MS.gene054610.t1 | MTR_3g100995 | 67.059 | 255 | 80 | 2 | 122 | 374 | 12 | 264 | 2.13e-119 | 346 |
| MS.gene054610.t1 | MTR_3g101190 | 51.667 | 360 | 114 | 3 | 15 | 374 | 13 | 312 | 1.07e-116 | 341 |
| MS.gene054610.t1 | MTR_7g050830 | 56.908 | 304 | 100 | 2 | 1 | 284 | 26 | 318 | 3.35e-114 | 335 |
| MS.gene054610.t1 | MTR_3g015760 | 62.313 | 268 | 91 | 6 | 112 | 372 | 15 | 279 | 8.99e-104 | 307 |
| MS.gene054610.t1 | MTR_3g100010 | 52.564 | 312 | 100 | 4 | 16 | 294 | 40 | 336 | 1.19e-101 | 304 |
| MS.gene054610.t1 | MTR_3g018747 | 58.621 | 261 | 87 | 3 | 117 | 372 | 9 | 253 | 1.29e-98 | 293 |
| MS.gene054610.t1 | MTR_3g111160 | 61.860 | 215 | 74 | 2 | 165 | 374 | 9 | 220 | 5.72e-83 | 252 |
| MS.gene054610.t1 | MTR_3g100520 | 59.193 | 223 | 71 | 4 | 158 | 374 | 1 | 209 | 1.40e-82 | 251 |
| MS.gene054610.t1 | MTR_3g099970 | 65.574 | 183 | 47 | 1 | 118 | 300 | 21 | 187 | 1.16e-78 | 240 |
| MS.gene054610.t1 | MTR_3g101110 | 57.021 | 235 | 64 | 5 | 123 | 348 | 74 | 280 | 4.22e-76 | 237 |
| MS.gene054610.t1 | MTR_3g114310 | 39.018 | 387 | 211 | 11 | 7 | 374 | 30 | 410 | 5.90e-73 | 233 |
| MS.gene054610.t1 | MTR_2g046410 | 57.488 | 207 | 77 | 3 | 174 | 372 | 144 | 347 | 1.10e-72 | 230 |
| MS.gene054610.t1 | MTR_3g101020 | 59.239 | 184 | 51 | 2 | 191 | 374 | 1 | 160 | 6.44e-65 | 204 |
| MS.gene054610.t1 | MTR_3g101150 | 67.089 | 158 | 45 | 2 | 223 | 373 | 1 | 158 | 2.25e-63 | 200 |
| MS.gene054610.t1 | MTR_3g102870 | 56.129 | 155 | 64 | 1 | 182 | 332 | 3 | 157 | 3.59e-53 | 174 |
| MS.gene054610.t1 | MTR_3g111150 | 47.953 | 171 | 63 | 3 | 1 | 171 | 9 | 153 | 2.54e-44 | 151 |
| MS.gene054610.t1 | MTR_3g100990 | 61.207 | 116 | 43 | 1 | 1 | 116 | 15 | 128 | 1.74e-36 | 139 |
| MS.gene054610.t1 | MTR_3g101140 | 56.303 | 119 | 41 | 2 | 256 | 374 | 9 | 116 | 9.32e-31 | 114 |
| MS.gene054610.t1 | MTR_4g093550 | 57.895 | 76 | 30 | 1 | 1 | 76 | 16 | 89 | 1.57e-21 | 88.6 |
| MS.gene054610.t1 | MTR_6g046890 | 61.667 | 60 | 23 | 0 | 15 | 74 | 21 | 80 | 1.59e-17 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054610.t1 | AT1G47710 | 48.677 | 378 | 186 | 4 | 1 | 372 | 15 | 390 | 2.20e-122 | 359 |
| MS.gene054610.t1 | AT1G47710 | 48.677 | 378 | 186 | 4 | 1 | 372 | 42 | 417 | 8.76e-122 | 358 |
| MS.gene054610.t1 | AT3G45220 | 45.924 | 368 | 179 | 4 | 17 | 371 | 30 | 390 | 6.55e-109 | 325 |
| MS.gene054610.t1 | AT2G25240 | 47.658 | 363 | 172 | 7 | 17 | 371 | 30 | 382 | 7.11e-108 | 322 |
| MS.gene054610.t1 | AT2G26390 | 46.322 | 367 | 174 | 8 | 17 | 371 | 31 | 386 | 1.31e-104 | 313 |
| MS.gene054610.t1 | AT1G62170 | 42.935 | 368 | 180 | 5 | 14 | 373 | 88 | 433 | 1.15e-97 | 297 |
| MS.gene054610.t1 | AT1G64030 | 42.933 | 375 | 192 | 5 | 1 | 371 | 15 | 371 | 7.80e-97 | 293 |
| MS.gene054610.t1 | AT2G14540 | 44.875 | 361 | 178 | 4 | 14 | 371 | 55 | 397 | 4.44e-95 | 290 |
| MS.gene054610.t1 | AT1G62170 | 39.651 | 401 | 179 | 6 | 14 | 373 | 88 | 466 | 1.46e-91 | 283 |
| MS.gene054610.t1 | AT2G35580 | 42.577 | 357 | 181 | 7 | 21 | 371 | 33 | 371 | 1.93e-91 | 279 |
| MS.gene054610.t1 | AT1G64010 | 47.907 | 215 | 91 | 3 | 158 | 371 | 1 | 195 | 8.35e-64 | 202 |
| MS.gene054610.t1 | AT1G64010 | 47.000 | 200 | 85 | 3 | 173 | 371 | 2 | 181 | 6.87e-56 | 181 |
| MS.gene054610.t1 | AT1G62160 | 41.000 | 200 | 91 | 5 | 180 | 373 | 42 | 220 | 1.00e-34 | 127 |
| MS.gene054610.t1 | AT1G63280 | 53.390 | 118 | 51 | 2 | 114 | 229 | 5 | 120 | 1.75e-34 | 124 |
| MS.gene054610.t1 | AT1G51330 | 49.107 | 112 | 54 | 2 | 115 | 223 | 29 | 140 | 1.48e-27 | 107 |
Find 85 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCCAATCAGTGAATTTA+TGG | 0.159817 | 3.4:-89085281 | MS.gene054610:CDS |
| TATGAGGAAGCTTGTCTTCT+AGG | 0.205127 | 3.4:+89084902 | None:intergenic |
| AACTGTTGCTATTCATTATA+TGG | 0.237017 | 3.4:-89085634 | MS.gene054610:CDS |
| CTTGTCTTTATATTCTTCTT+TGG | 0.244807 | 3.4:+89085905 | None:intergenic |
| TGTCTTCTGCCAACGCTGTT+TGG | 0.271717 | 3.4:-89085695 | MS.gene054610:CDS |
| CCTCTTCATGCTAGTCATAA+AGG | 0.293948 | 3.4:+89085077 | None:intergenic |
| GTCTTCTGCCAACGCTGTTT+GGG | 0.300962 | 3.4:-89085694 | MS.gene054610:CDS |
| GATGCAAAAGATGGGTTGTT+AGG | 0.331478 | 3.4:-89084958 | MS.gene054610:CDS |
| CGGAAGCGACGTGAGAAGAT+AGG | 0.331599 | 3.4:+89085757 | None:intergenic |
| GCTGCCGCATTCACTGGAAT+TGG | 0.345550 | 3.4:-89084682 | MS.gene054610:CDS |
| TCATCTTCACCTTGCTTGTA+AGG | 0.348281 | 3.4:+89085012 | None:intergenic |
| GGAGCAATCGTTTGATGCTT+CGG | 0.373313 | 3.4:-89085151 | MS.gene054610:CDS |
| CGAGGAGACTGAGCGCAGTT+TGG | 0.373483 | 3.4:+89085856 | None:intergenic |
| TCTGATGTGTTGAAGGAGTT+AGG | 0.381460 | 3.4:-89084817 | MS.gene054610:CDS |
| AGCCCATAAATTCACTGATT+GGG | 0.386856 | 3.4:+89085278 | None:intergenic |
| AATAGCAACAGTTTCTTTGA+AGG | 0.390569 | 3.4:+89085644 | None:intergenic |
| AATACTGTAGAAACACTAAT+TGG | 0.391329 | 3.4:+89084745 | None:intergenic |
| CAGCCCATAAATTCACTGAT+TGG | 0.401585 | 3.4:+89085277 | None:intergenic |
| CTGCTCCTGCCGGCGGTCCA+TGG | 0.408400 | 3.4:-89085719 | MS.gene054610:CDS |
| TCTGGTACAATACTCTTTGT+TGG | 0.408824 | 3.4:-89084568 | MS.gene054610:CDS |
| TGAAATTTCTGATGTGTTGA+AGG | 0.418942 | 3.4:-89084824 | MS.gene054610:CDS |
| ATTCTTCTTTGGAGAACAAA+TGG | 0.423124 | 3.4:+89085916 | None:intergenic |
| CTCTTCATGCTAGTCATAAA+GGG | 0.432869 | 3.4:+89085078 | None:intergenic |
| TTTCTTCCAGATGCAAAAGA+TGG | 0.444703 | 3.4:-89084967 | MS.gene054610:CDS |
| CTGCCGCATTCACTGGAATT+GGG | 0.449429 | 3.4:-89084681 | MS.gene054610:CDS |
| AGTGAATTTATGGGCTGAAA+AGG | 0.450449 | 3.4:-89085271 | MS.gene054610:CDS |
| TTCACTGGAATTGGGATGGC+TGG | 0.452119 | 3.4:-89084673 | MS.gene054610:CDS |
| TTGATTAGAGAAGATTTGTC+TGG | 0.453212 | 3.4:-89084586 | MS.gene054610:CDS |
| AACCCAATCAGTGAATTTAT+GGG | 0.457167 | 3.4:-89085280 | MS.gene054610:CDS |
| GGAAGCGACGTGAGAAGATA+GGG | 0.462599 | 3.4:+89085758 | None:intergenic |
| CAGCGAGGGGATTATGCACT+TGG | 0.465525 | 3.4:+89084545 | None:intergenic |
| CATTATACTTCAAAGGAGCT+TGG | 0.467015 | 3.4:-89085173 | MS.gene054610:CDS |
| CTCCTCGCTGTTGGTTCTGA+GGG | 0.470422 | 3.4:-89085840 | MS.gene054610:CDS |
| TCAAAAGAAATATTGAATCT+TGG | 0.470594 | 3.4:+89084850 | None:intergenic |
| TGAAGGAAGGGTAAACAGAA+AGG | 0.471470 | 3.4:+89085661 | None:intergenic |
| TGGGCTGAAAAGGAGACAAA+AGG | 0.472940 | 3.4:-89085261 | MS.gene054610:CDS |
| ACTAAAGCTGCCGCATTCAC+TGG | 0.473621 | 3.4:-89084688 | MS.gene054610:CDS |
| GTCAAACCTCCAATAGAGAA+AGG | 0.475976 | 3.4:+89084784 | None:intergenic |
| ATTATACTTCAAAGGAGCTT+GGG | 0.479823 | 3.4:-89085172 | MS.gene054610:CDS |
| CAACAGTTTCTTTGAAGGAA+GGG | 0.487406 | 3.4:+89085649 | None:intergenic |
| TCTCCTCGCTGTTGGTTCTG+AGG | 0.490976 | 3.4:-89085841 | MS.gene054610:CDS |
| GAAGGAAGGGTAAACAGAAA+GGG | 0.492254 | 3.4:+89085662 | None:intergenic |
| CTCAAAGACGCTGCTCCTGC+CGG | 0.500555 | 3.4:-89085729 | MS.gene054610:CDS |
| GCAACAGTTTCTTTGAAGGA+AGG | 0.503967 | 3.4:+89085648 | None:intergenic |
| TTCTTTGGAGAACAAATGGT+TGG | 0.505452 | 3.4:+89085920 | None:intergenic |
| GCGCTCAGTCTCCTCGCTGT+TGG | 0.506186 | 3.4:-89085849 | MS.gene054610:CDS |
| GGTATCGGAGTTGAAGTGTA+TGG | 0.511402 | 3.4:+89084643 | None:intergenic |
| TGGTCAGCTACAAAGTCTAT+TGG | 0.513464 | 3.4:+89084622 | None:intergenic |
| ACAAATGCATTATACTTCAA+AGG | 0.517977 | 3.4:-89085180 | MS.gene054610:CDS |
| GTCATTGAAGTAAATGAAGA+AGG | 0.521214 | 3.4:-89084712 | MS.gene054610:CDS |
| TTCTTCCAGATGCAAAAGAT+GGG | 0.522138 | 3.4:-89084966 | MS.gene054610:CDS |
| GACAACCATGGACCGCCGGC+AGG | 0.522330 | 3.4:+89085714 | None:intergenic |
| CGCATTCACTGGAATTGGGA+TGG | 0.526890 | 3.4:-89084677 | MS.gene054610:CDS |
| GATGGACTTTGATTCGAGAA+AGG | 0.529577 | 3.4:+89085794 | None:intergenic |
| GGGCTGAAAAGGAGACAAAA+GGG | 0.540477 | 3.4:-89085260 | MS.gene054610:CDS |
| AGCGCAGTTTGGAGCGACAA+TGG | 0.549289 | 3.4:+89085867 | None:intergenic |
| GCTACAAAGTCTATTGGTAT+CGG | 0.556429 | 3.4:+89084628 | None:intergenic |
| CTTAGTCTTCCTTACAAGCA+AGG | 0.563134 | 3.4:-89085021 | MS.gene054610:CDS |
| GTTAGGTTTGATTGAGAAAG+CGG | 0.567281 | 3.4:-89084941 | MS.gene054610:CDS |
| AGAAGACAACCATGGACCGC+CGG | 0.567508 | 3.4:+89085710 | None:intergenic |
| GGAGAACAAATGGTTGGCGA+TGG | 0.571262 | 3.4:+89085926 | None:intergenic |
| CCTTTATGACTAGCATGAAG+AGG | 0.571406 | 3.4:-89085077 | MS.gene054610:CDS |
| CAAGCTTCCTCATACAAAAG+TGG | 0.592026 | 3.4:-89084893 | MS.gene054610:CDS |
| CAAGTGCATAATCCCCTCGC+TGG | 0.595687 | 3.4:-89084544 | MS.gene054610:CDS |
| TCTCAATGGCACCTCAGTCA+AGG | 0.599345 | 3.4:-89085103 | MS.gene054610:CDS |
| TAGGGAGTTGAGATGGCCGA+TGG | 0.601672 | 3.4:+89085776 | None:intergenic |
| CTTGCTTGTAAGGAAGACTA+AGG | 0.608424 | 3.4:+89085022 | None:intergenic |
| GAGAAGATAGGGAGTTGAGA+TGG | 0.618975 | 3.4:+89085769 | None:intergenic |
| AAAGACGCTGCTCCTGCCGG+CGG | 0.624527 | 3.4:-89085726 | MS.gene054610:CDS |
| GAAATCAAGTGATTTGAGAG+TGG | 0.625530 | 3.4:+89085611 | None:intergenic |
| CCTCATACAAAAGTGGTGGT+AGG | 0.629641 | 3.4:-89084886 | MS.gene054610:CDS |
| CATCCCAATTCCAGTGAATG+CGG | 0.632365 | 3.4:+89084678 | None:intergenic |
| TGTGTTGAAGGAGTTAGGAG+TGG | 0.642269 | 3.4:-89084812 | MS.gene054610:CDS |
| TAGTTGAGATCATCCAGCGA+GGG | 0.653122 | 3.4:+89084531 | None:intergenic |
| TTTCTCGAATCAAAGTCCAT+CGG | 0.660158 | 3.4:-89085792 | MS.gene054610:CDS |
| GTTAAGCTATTAATCGACCC+AGG | 0.661094 | 3.4:+89085216 | None:intergenic |
| AGCAGCGTCTTTGAGAACAG+CGG | 0.665031 | 3.4:+89085737 | None:intergenic |
| TGACTGAGGTGCCATTGAGA+AGG | 0.673594 | 3.4:+89085106 | None:intergenic |
| GCTTCCTCATACAAAAGTGG+TGG | 0.680566 | 3.4:-89084890 | MS.gene054610:CDS |
| AGTTGAGATCATCCAGCGAG+GGG | 0.687329 | 3.4:+89084532 | None:intergenic |
| CATAAAGGGAACCTTGACTG+AGG | 0.721556 | 3.4:+89085092 | None:intergenic |
| GACCCTCAGAACCAACAGCG+AGG | 0.731995 | 3.4:+89085838 | None:intergenic |
| AAGCTATTAATCGACCCAGG+TGG | 0.746620 | 3.4:+89085219 | None:intergenic |
| GCGTTGGCAGAAGACAACCA+TGG | 0.760268 | 3.4:+89085702 | None:intergenic |
| GTAGTTGAGATCATCCAGCG+AGG | 0.762097 | 3.4:+89084530 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTTTAGAATAGAAAATA+AGG | + | chr3.4:89084970-89084989 | None:intergenic | 15.0% |
| !!! | TATATTTGATATTGTTTTGA+AGG | - | chr3.4:89085157-89085176 | MS.gene054610:CDS | 15.0% |
| !! | TCAAAAGAAATATTGAATCT+TGG | + | chr3.4:89085623-89085642 | None:intergenic | 20.0% |
| !! | TTAATCTACTAATCTAACAA+GGG | + | chr3.4:89084941-89084960 | None:intergenic | 20.0% |
| !! | TTTAATCTACTAATCTAACA+AGG | + | chr3.4:89084942-89084961 | None:intergenic | 20.0% |
| !!! | TATTTTGTTGTTAAATTGCT+TGG | + | chr3.4:89085048-89085067 | None:intergenic | 20.0% |
| ! | AATACTGTAGAAACACTAAT+TGG | + | chr3.4:89085728-89085747 | None:intergenic | 25.0% |
| ! | ACAAATGCATTATACTTCAA+AGG | - | chr3.4:89085290-89085309 | MS.gene054610:CDS | 25.0% |
| ! | CTCTAATCAAAAACAAGAAA+GGG | + | chr3.4:89085874-89085893 | None:intergenic | 25.0% |
| ! | CTTGTCTTTATATTCTTCTT+TGG | + | chr3.4:89084568-89084587 | None:intergenic | 25.0% |
| ! | TCTCTAATCAAAAACAAGAA+AGG | + | chr3.4:89085875-89085894 | None:intergenic | 25.0% |
| !! | AACTGTTGCTATTCATTATA+TGG | - | chr3.4:89084836-89084855 | MS.gene054610:CDS | 25.0% |
| AACCCAATCAGTGAATTTAT+GGG | - | chr3.4:89085190-89085209 | MS.gene054610:CDS | 30.0% | |
| AATAGCAACAGTTTCTTTGA+AGG | + | chr3.4:89084829-89084848 | None:intergenic | 30.0% | |
| ATTATACTTCAAAGGAGCTT+GGG | - | chr3.4:89085298-89085317 | MS.gene054610:intron | 30.0% | |
| GTCATTGAAGTAAATGAAGA+AGG | - | chr3.4:89085758-89085777 | MS.gene054610:CDS | 30.0% | |
| TAATCAAAAACAAGAAAGGG+TGG | + | chr3.4:89085871-89085890 | None:intergenic | 30.0% | |
| TGAAATTTCTGATGTGTTGA+AGG | - | chr3.4:89085646-89085665 | MS.gene054610:CDS | 30.0% | |
| TTGATTAGAGAAGATTTGTC+TGG | - | chr3.4:89085884-89085903 | MS.gene054610:CDS | 30.0% | |
| ! | ATTCTTCTTTGGAGAACAAA+TGG | + | chr3.4:89084557-89084576 | None:intergenic | 30.0% |
| CAACCCAATCAGTGAATTTA+TGG | - | chr3.4:89085189-89085208 | MS.gene054610:CDS | 35.0% | |
| CATTATACTTCAAAGGAGCT+TGG | - | chr3.4:89085297-89085316 | MS.gene054610:intron | 35.0% | |
| CTCTTCATGCTAGTCATAAA+GGG | + | chr3.4:89085395-89085414 | None:intergenic | 35.0% | |
| GAAATCAAGTGATTTGAGAG+TGG | + | chr3.4:89084862-89084881 | None:intergenic | 35.0% | |
| GCTACAAAGTCTATTGGTAT+CGG | + | chr3.4:89085845-89085864 | None:intergenic | 35.0% | |
| GTTAGGTTTGATTGAGAAAG+CGG | - | chr3.4:89085529-89085548 | MS.gene054610:intron | 35.0% | |
| TCTGGTACAATACTCTTTGT+TGG | - | chr3.4:89085902-89085921 | MS.gene054610:CDS | 35.0% | |
| TGGAGGTTTGACAAAAATAG+TGG | - | chr3.4:89085697-89085716 | MS.gene054610:CDS | 35.0% | |
| TTCTTCCAGATGCAAAAGAT+GGG | - | chr3.4:89085504-89085523 | MS.gene054610:intron | 35.0% | |
| TTTCTCGAATCAAAGTCCAT+CGG | - | chr3.4:89084678-89084697 | MS.gene054610:CDS | 35.0% | |
| TTTCTTCCAGATGCAAAAGA+TGG | - | chr3.4:89085503-89085522 | MS.gene054610:intron | 35.0% | |
| ! | AGCCCATAAATTCACTGATT+GGG | + | chr3.4:89085195-89085214 | None:intergenic | 35.0% |
| ! | AGTGAATTTATGGGCTGAAA+AGG | - | chr3.4:89085199-89085218 | MS.gene054610:CDS | 35.0% |
| ! | ATTCGTAATTTTCTTCCACC+TGG | - | chr3.4:89085236-89085255 | MS.gene054610:CDS | 35.0% |
| ! | CAACAGTTTCTTTGAAGGAA+GGG | + | chr3.4:89084824-89084843 | None:intergenic | 35.0% |
| ! | CAGTTTATCAGTGCTTTTGA+TGG | - | chr3.4:89085416-89085435 | MS.gene054610:intron | 35.0% |
| ! | GTTTTACCTTTCTCTATTGG+AGG | - | chr3.4:89085680-89085699 | MS.gene054610:CDS | 35.0% |
| ! | TACGATTTTCACCTTCTCAA+TGG | - | chr3.4:89085353-89085372 | MS.gene054610:intron | 35.0% |
| ! | TTCGTAATTTTCTTCCACCT+GGG | - | chr3.4:89085237-89085256 | MS.gene054610:CDS | 35.0% |
| ! | TTCTTTGGAGAACAAATGGT+TGG | + | chr3.4:89084553-89084572 | None:intergenic | 35.0% |
| !! | GTGGTTTTACCTTTCTCTAT+TGG | - | chr3.4:89085677-89085696 | MS.gene054610:CDS | 35.0% |
| CAAGCTTCCTCATACAAAAG+TGG | - | chr3.4:89085577-89085596 | MS.gene054610:intron | 40.0% | |
| CAGCCCATAAATTCACTGAT+TGG | + | chr3.4:89085196-89085215 | None:intergenic | 40.0% | |
| CCTCTTCATGCTAGTCATAA+AGG | + | chr3.4:89085396-89085415 | None:intergenic | 40.0% | |
| CCTTTATGACTAGCATGAAG+AGG | - | chr3.4:89085393-89085412 | MS.gene054610:intron | 40.0% | |
| CTTGCTTGTAAGGAAGACTA+AGG | + | chr3.4:89085451-89085470 | None:intergenic | 40.0% | |
| GAAGGAAGGGTAAACAGAAA+GGG | + | chr3.4:89084811-89084830 | None:intergenic | 40.0% | |
| GTCAAACCTCCAATAGAGAA+AGG | + | chr3.4:89085689-89085708 | None:intergenic | 40.0% | |
| GTTAAGCTATTAATCGACCC+AGG | + | chr3.4:89085257-89085276 | None:intergenic | 40.0% | |
| TATGAGGAAGCTTGTCTTCT+AGG | + | chr3.4:89085571-89085590 | None:intergenic | 40.0% | |
| TCATCTTCACCTTGCTTGTA+AGG | + | chr3.4:89085461-89085480 | None:intergenic | 40.0% | |
| TCTGATGTGTTGAAGGAGTT+AGG | - | chr3.4:89085653-89085672 | MS.gene054610:CDS | 40.0% | |
| TGAAGGAAGGGTAAACAGAA+AGG | + | chr3.4:89084812-89084831 | None:intergenic | 40.0% | |
| TGGTCAGCTACAAAGTCTAT+TGG | + | chr3.4:89085851-89085870 | None:intergenic | 40.0% | |
| ! | AACAACCCATCTTTTGCATC+TGG | + | chr3.4:89085512-89085531 | None:intergenic | 40.0% |
| ! | CTTAGTCTTCCTTACAAGCA+AGG | - | chr3.4:89085449-89085468 | MS.gene054610:intron | 40.0% |
| ! | GATGGACTTTGATTCGAGAA+AGG | + | chr3.4:89084679-89084698 | None:intergenic | 40.0% |
| ! | GCAACAGTTTCTTTGAAGGA+AGG | + | chr3.4:89084825-89084844 | None:intergenic | 40.0% |
| !! | CAGTGCTTTTGATGGCTTTA+AGG | - | chr3.4:89085424-89085443 | MS.gene054610:intron | 40.0% |
| !! | GACAGAAGCTGATTTTGAGT+GGG | + | chr3.4:89084657-89084676 | None:intergenic | 40.0% |
| !! | GATGCAAAAGATGGGTTGTT+AGG | - | chr3.4:89085512-89085531 | MS.gene054610:intron | 40.0% |
| !! | TTTTTCAACCCAAACAGCGT+TGG | + | chr3.4:89084787-89084806 | None:intergenic | 40.0% |
| AAGCTATTAATCGACCCAGG+TGG | + | chr3.4:89085254-89085273 | None:intergenic | 45.0% | |
| ACTACCATACAACAGTTGCG+AGG | + | chr3.4:89084998-89085017 | None:intergenic | 45.0% | |
| CATAAAGGGAACCTTGACTG+AGG | + | chr3.4:89085381-89085400 | None:intergenic | 45.0% | |
| CATCCCAATTCCAGTGAATG+CGG | + | chr3.4:89085795-89085814 | None:intergenic | 45.0% | |
| CCTCATACAAAAGTGGTGGT+AGG | - | chr3.4:89085584-89085603 | MS.gene054610:intron | 45.0% | |
| GAGAAGATAGGGAGTTGAGA+TGG | + | chr3.4:89084704-89084723 | None:intergenic | 45.0% | |
| GCTTCCTCATACAAAAGTGG+TGG | - | chr3.4:89085580-89085599 | MS.gene054610:intron | 45.0% | |
| GGAGCAATCGTTTGATGCTT+CGG | - | chr3.4:89085319-89085338 | MS.gene054610:intron | 45.0% | |
| GGGCTGAAAAGGAGACAAAA+GGG | - | chr3.4:89085210-89085229 | MS.gene054610:CDS | 45.0% | |
| TGGGCTGAAAAGGAGACAAA+AGG | - | chr3.4:89085209-89085228 | MS.gene054610:CDS | 45.0% | |
| TGTGTTGAAGGAGTTAGGAG+TGG | - | chr3.4:89085658-89085677 | MS.gene054610:CDS | 45.0% | |
| ! | GGTATCGGAGTTGAAGTGTA+TGG | + | chr3.4:89085830-89085849 | None:intergenic | 45.0% |
| !! | CCTACCACCACTTTTGTATG+AGG | + | chr3.4:89085587-89085606 | None:intergenic | 45.0% |
| !! | GGACAGAAGCTGATTTTGAG+TGG | + | chr3.4:89084658-89084677 | None:intergenic | 45.0% |
| ACTAAAGCTGCCGCATTCAC+TGG | - | chr3.4:89085782-89085801 | MS.gene054610:CDS | 50.0% | |
| CATACAACAGTTGCGAGGCA+TGG | + | chr3.4:89084993-89085012 | None:intergenic | 50.0% | |
| CATGCCTCGCAACTGTTGTA+TGG | - | chr3.4:89084991-89085010 | MS.gene054610:CDS | 50.0% | |
| CGCATTCACTGGAATTGGGA+TGG | - | chr3.4:89085793-89085812 | MS.gene054610:CDS | 50.0% | |
| CTGCCGCATTCACTGGAATT+GGG | - | chr3.4:89085789-89085808 | MS.gene054610:CDS | 50.0% | |
| GGAAGCGACGTGAGAAGATA+GGG | + | chr3.4:89084715-89084734 | None:intergenic | 50.0% | |
| GGAGAACAAATGGTTGGCGA+TGG | + | chr3.4:89084547-89084566 | None:intergenic | 50.0% | |
| GTCTTCTGCCAACGCTGTTT+GGG | - | chr3.4:89084776-89084795 | MS.gene054610:CDS | 50.0% | |
| TCTCAATGGCACCTCAGTCA+AGG | - | chr3.4:89085367-89085386 | MS.gene054610:intron | 50.0% | |
| TGTCTTCTGCCAACGCTGTT+TGG | - | chr3.4:89084775-89084794 | MS.gene054610:CDS | 50.0% | |
| TTCACTGGAATTGGGATGGC+TGG | - | chr3.4:89085797-89085816 | MS.gene054610:CDS | 50.0% | |
| ! | AGCAGCGTCTTTGAGAACAG+CGG | + | chr3.4:89084736-89084755 | None:intergenic | 50.0% |
| !! | TGACTGAGGTGCCATTGAGA+AGG | + | chr3.4:89085367-89085386 | None:intergenic | 50.0% |
| AGAAGACAACCATGGACCGC+CGG | + | chr3.4:89084763-89084782 | None:intergenic | 55.0% | |
| CAAGTGCATAATCCCCTCGC+TGG | - | chr3.4:89085926-89085945 | MS.gene054610:CDS | 55.0% | |
| CAGCGAGGGGATTATGCACT+TGG | + | chr3.4:89085928-89085947 | None:intergenic | 55.0% | |
| CGGAAGCGACGTGAGAAGAT+AGG | + | chr3.4:89084716-89084735 | None:intergenic | 55.0% | |
| GCTGCCGCATTCACTGGAAT+TGG | - | chr3.4:89085788-89085807 | MS.gene054610:CDS | 55.0% | |
| TAGGGAGTTGAGATGGCCGA+TGG | + | chr3.4:89084697-89084716 | None:intergenic | 55.0% | |
| ! | AGCGCAGTTTGGAGCGACAA+TGG | + | chr3.4:89084606-89084625 | None:intergenic | 55.0% |
| ! | GCGTTGGCAGAAGACAACCA+TGG | + | chr3.4:89084771-89084790 | None:intergenic | 55.0% |
| !! | CTCCTCGCTGTTGGTTCTGA+GGG | - | chr3.4:89084630-89084649 | MS.gene054610:CDS | 55.0% |
| !! | TCTCCTCGCTGTTGGTTCTG+AGG | - | chr3.4:89084629-89084648 | MS.gene054610:CDS | 55.0% |
| CGAGGAGACTGAGCGCAGTT+TGG | + | chr3.4:89084617-89084636 | None:intergenic | 60.0% | |
| CTCAAAGACGCTGCTCCTGC+CGG | - | chr3.4:89084741-89084760 | MS.gene054610:CDS | 60.0% | |
| GACCCTCAGAACCAACAGCG+AGG | + | chr3.4:89084635-89084654 | None:intergenic | 60.0% | |
| AAAGACGCTGCTCCTGCCGG+CGG | - | chr3.4:89084744-89084763 | MS.gene054610:CDS | 65.0% | |
| GCGCTCAGTCTCCTCGCTGT+TGG | - | chr3.4:89084621-89084640 | MS.gene054610:CDS | 65.0% | |
| GACAACCATGGACCGCCGGC+AGG | + | chr3.4:89084759-89084778 | None:intergenic | 70.0% | |
| CTGCTCCTGCCGGCGGTCCA+TGG | - | chr3.4:89084751-89084770 | MS.gene054610:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 89084540 | 89085952 | 89084540 | ID=MS.gene054610 |
| chr3.4 | mRNA | 89084540 | 89085952 | 89084540 | ID=MS.gene054610.t1;Parent=MS.gene054610 |
| chr3.4 | exon | 89085602 | 89085952 | 89085602 | ID=MS.gene054610.t1.exon1;Parent=MS.gene054610.t1 |
| chr3.4 | CDS | 89085602 | 89085952 | 89085602 | ID=cds.MS.gene054610.t1;Parent=MS.gene054610.t1 |
| chr3.4 | exon | 89084540 | 89085313 | 89084540 | ID=MS.gene054610.t1.exon2;Parent=MS.gene054610.t1 |
| chr3.4 | CDS | 89084540 | 89085313 | 89084540 | ID=cds.MS.gene054610.t1;Parent=MS.gene054610.t1 |
| Gene Sequence |
| Protein sequence |
