Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48315.t1 | XP_013457733.1 | 98.1 | 206 | 4 | 0 | 1 | 206 | 1 | 206 | 1.40E-109 | 405.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48315.t1 | Q9FGS5 | 52.6 | 209 | 88 | 5 | 1 | 202 | 1 | 205 | 1.4e-49 | 197.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48315.t1 | I3SWZ8 | 98.1 | 206 | 4 | 0 | 1 | 206 | 1 | 206 | 9.9e-110 | 405.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050353 | MS.gene48315 | 0.806792 | 6.93E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene48315 | 0.800165 | 1.64E-48 | -1.69E-46 |
| MS.gene058840 | MS.gene48315 | 0.831115 | 2.00E-55 | -1.69E-46 |
| MS.gene059943 | MS.gene48315 | 0.911083 | 9.08E-83 | -1.69E-46 |
| MS.gene059944 | MS.gene48315 | 0.807729 | 4.38E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48315.t1 | MTR_4g104730 | 98.058 | 206 | 4 | 0 | 1 | 206 | 1 | 206 | 1.78e-153 | 423 |
| MS.gene48315.t1 | MTR_4g104700 | 97.573 | 206 | 5 | 0 | 1 | 206 | 1 | 206 | 6.13e-153 | 422 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48315.t1 | AT5G50200 | 52.632 | 209 | 88 | 5 | 1 | 202 | 1 | 205 | 4.34e-68 | 207 |
| MS.gene48315.t1 | AT5G50200 | 52.632 | 209 | 88 | 5 | 1 | 202 | 1 | 205 | 4.34e-68 | 207 |
| MS.gene48315.t1 | AT5G50200 | 52.632 | 209 | 88 | 5 | 1 | 202 | 1 | 205 | 4.34e-68 | 207 |
| MS.gene48315.t1 | AT4G24715 | 50.725 | 207 | 94 | 5 | 1 | 202 | 23 | 226 | 1.69e-65 | 202 |
Find 50 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGCACAATTTCAGCTTTA+TGG | 0.108638 | 8.3:-17780519 | None:intergenic |
| ACCTATGTAGTTCTGTTATC+TGG | 0.283515 | 8.3:+17780425 | MS.gene48315:intron |
| CGTTGTCTTCAGTGCTTTCT+CGG | 0.301197 | 8.3:+17780841 | MS.gene48315:CDS |
| TACTTACCTTGTCCATGCTT+AGG | 0.361592 | 8.3:-17779875 | None:intergenic |
| ACTTACCTTGTCCATGCTTA+GGG | 0.362581 | 8.3:-17779874 | None:intergenic |
| TCTTACCCGCGTTCGTGGTT+TGG | 0.381755 | 8.3:-17780757 | None:intergenic |
| ACTGAATTCAATTACTGCTT+TGG | 0.387524 | 8.3:-17780914 | None:intergenic |
| GAATCAACCACTCGAAGGTT+TGG | 0.404136 | 8.3:-17780658 | None:intergenic |
| AACGATAAGGAAGTGGCTTA+TGG | 0.411170 | 8.3:+17780734 | MS.gene48315:CDS |
| TGTTTAGCTGAAATTTGTTA+TGG | 0.421382 | 8.3:+17779803 | MS.gene48315:CDS |
| TGCATTTGATTCCAACGATA+AGG | 0.421816 | 8.3:+17780721 | MS.gene48315:CDS |
| GCTTTCTCGGTTGTGTCCCT+AGG | 0.448319 | 8.3:+17780854 | MS.gene48315:CDS |
| GATTCAGCGCGACGTGCCTC+AGG | 0.452711 | 8.3:+17780676 | MS.gene48315:CDS |
| GGTACTCTTACCCGCGTTCG+TGG | 0.474972 | 8.3:-17780762 | None:intergenic |
| TATGAAGAATCTGTTCCAGC+TGG | 0.476881 | 8.3:-17780491 | None:intergenic |
| AGCTGAAATTTGTTATGGAA+AGG | 0.496497 | 8.3:+17779808 | MS.gene48315:CDS |
| ATAAGCCACTTCCTTATCGT+TGG | 0.499427 | 8.3:-17780732 | None:intergenic |
| AGACCGAGGATGAACTCTCT+AGG | 0.513840 | 8.3:+17780576 | MS.gene48315:CDS |
| TGCATTGATCTCAAACAAGT+TGG | 0.514725 | 8.3:-17780783 | None:intergenic |
| TGATTCCAACGATAAGGAAG+TGG | 0.521314 | 8.3:+17780727 | MS.gene48315:CDS |
| CTAAACAAGACCTTCCCAGC+TGG | 0.523958 | 8.3:+17780476 | MS.gene48315:CDS |
| GTTGGAAGCATTGTAGGGCA+TGG | 0.526614 | 8.3:-17780627 | None:intergenic |
| TAGGGGAAGCTGTGACCTCA+AGG | 0.526901 | 8.3:-17779856 | None:intergenic |
| AGAATCTGTTCCAGCTGGGA+AGG | 0.546736 | 8.3:-17780486 | None:intergenic |
| CTACAATGCTTCCAACAAAA+CGG | 0.547064 | 8.3:+17780634 | MS.gene48315:CDS |
| GTCCCTAGAGAGTTCATCCT+CGG | 0.558273 | 8.3:-17780579 | None:intergenic |
| GACCGAGGATGAACTCTCTA+GGG | 0.561011 | 8.3:+17780577 | MS.gene48315:CDS |
| GTCACAGCTTCCCCTAAGCA+TGG | 0.584742 | 8.3:+17779863 | MS.gene48315:CDS |
| CAATATAGAAGAACACACCT+AGG | 0.601286 | 8.3:-17780871 | None:intergenic |
| TAGACAAAATCAGTGGAACA+TGG | 0.608919 | 8.3:+17780450 | MS.gene48315:CDS |
| CTTACCTTGTCCATGCTTAG+GGG | 0.615805 | 8.3:-17779873 | None:intergenic |
| CGAGAAAGCACTGAAGACAA+CGG | 0.621934 | 8.3:-17780840 | None:intergenic |
| TTCTTTGAAAAGAACCCTTG+AGG | 0.622622 | 8.3:+17779841 | MS.gene48315:CDS |
| ATCGTTGGAATCAAATGCAT+AGG | 0.623735 | 8.3:-17780717 | None:intergenic |
| ATGAAGAATCTGTTCCAGCT+GGG | 0.623941 | 8.3:-17780490 | None:intergenic |
| TTTGAGATCAATGCAATCAG+TGG | 0.626853 | 8.3:+17780791 | MS.gene48315:CDS |
| TATGGCCAAACCACGAACGC+GGG | 0.634750 | 8.3:+17780752 | MS.gene48315:CDS |
| TTATGGCCAAACCACGAACG+CGG | 0.634987 | 8.3:+17780751 | MS.gene48315:CDS |
| AGGGGAAGCTGTGACCTCAA+GGG | 0.635053 | 8.3:-17779855 | None:intergenic |
| GCGCTGAATCAACCACTCGA+AGG | 0.649799 | 8.3:-17780663 | None:intergenic |
| CCTCGGTCTTTCTCCATGCG+CGG | 0.651243 | 8.3:-17780562 | None:intergenic |
| AATATAGAAGAACACACCTA+GGG | 0.653049 | 8.3:-17780870 | None:intergenic |
| TATGGCAGCACACAAGCTTG+TGG | 0.654698 | 8.3:+17779760 | None:intergenic |
| TCTGGAGTAGACAAAATCAG+TGG | 0.660740 | 8.3:+17780443 | MS.gene48315:CDS |
| GCTTCCCCTAAGCATGGACA+AGG | 0.668529 | 8.3:+17779869 | MS.gene48315:CDS |
| CGTACAAAGTACGTTGCCTG+AGG | 0.678441 | 8.3:-17780692 | None:intergenic |
| AGCAGATATCAAGAGTCGCG+TGG | 0.681698 | 8.3:-17780817 | None:intergenic |
| AAACGGTCCAAACCTTCGAG+TGG | 0.693663 | 8.3:+17780651 | MS.gene48315:CDS |
| AGATATCAAGAGTCGCGTGG+CGG | 0.700580 | 8.3:-17780814 | None:intergenic |
| CCGCGCATGGAGAAAGACCG+AGG | 0.703551 | 8.3:+17780562 | MS.gene48315:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTCTTTTCTTTAAAA+AGG | + | chr8.3:17780006-17780025 | MS.gene48315:intron | 10.0% |
| !! | GATAAGACATTCATTTATTA+AGG | - | chr8.3:17780316-17780335 | None:intergenic | 20.0% |
| !! | TGTTCTTCTATATTGAAAAA+AGG | + | chr8.3:17780879-17780898 | MS.gene48315:CDS | 20.0% |
| !! | TTCTATATTGAAAAAAGGAA+GGG | + | chr8.3:17780884-17780903 | MS.gene48315:CDS | 20.0% |
| !!! | GAACATTTTTCAAGAAAATT+TGG | + | chr8.3:17780150-17780169 | MS.gene48315:intron | 20.0% |
| ! | ATAATCACATAAATCAAGGT+TGG | - | chr8.3:17780084-17780103 | None:intergenic | 25.0% |
| ! | CTATTCAAATTCAAGAAAAG+AGG | - | chr8.3:17780114-17780133 | None:intergenic | 25.0% |
| ! | CTTCTATATTGAAAAAAGGA+AGG | + | chr8.3:17780883-17780902 | MS.gene48315:CDS | 25.0% |
| ! | GAACATAATCACATAAATCA+AGG | - | chr8.3:17780088-17780107 | None:intergenic | 25.0% |
| ! | GATATTACAAATGATCACAA+AGG | + | chr8.3:17780240-17780259 | MS.gene48315:intron | 25.0% |
| ! | TATTAACGTTGCAGAAATTA+AGG | - | chr8.3:17780282-17780301 | None:intergenic | 25.0% |
| ! | TTCTTGAAAAATGTTCATAC+TGG | - | chr8.3:17780146-17780165 | None:intergenic | 25.0% |
| !!! | TGTTTAGCTGAAATTTGTTA+TGG | + | chr8.3:17779803-17779822 | MS.gene48315:CDS | 25.0% |
| AACCTTCTTAAATAACACAC+TGG | - | chr8.3:17780192-17780211 | None:intergenic | 30.0% | |
| AACTAGCATAAGTCAATACA+AGG | - | chr8.3:17779917-17779936 | None:intergenic | 30.0% | |
| AATATAGAAGAACACACCTA+GGG | - | chr8.3:17780873-17780892 | None:intergenic | 30.0% | |
| CAAGTATGCAATCACAATAT+AGG | - | chr8.3:17780338-17780357 | None:intergenic | 30.0% | |
| TCAAATTCAAGAAAAGAGGA+AGG | - | chr8.3:17780110-17780129 | None:intergenic | 30.0% | |
| ! | AAGCTGATTAAACTCGATTT+TGG | + | chr8.3:17780394-17780413 | MS.gene48315:intron | 30.0% |
| !! | AGCTGAAATTTGTTATGGAA+AGG | + | chr8.3:17779808-17779827 | MS.gene48315:CDS | 30.0% |
| ACACTGGACTCTATTAAACT+TGG | - | chr8.3:17780176-17780195 | None:intergenic | 35.0% | |
| ATCGAGTTTAATCAGCTTGT+TGG | - | chr8.3:17780392-17780411 | None:intergenic | 35.0% | |
| ATCGTTGGAATCAAATGCAT+AGG | - | chr8.3:17780720-17780739 | None:intergenic | 35.0% | |
| CAATATAGAAGAACACACCT+AGG | - | chr8.3:17780874-17780893 | None:intergenic | 35.0% | |
| CTACAATGCTTCCAACAAAA+CGG | + | chr8.3:17780634-17780653 | MS.gene48315:CDS | 35.0% | |
| GTAGCACAATTTCAGCTTTA+TGG | - | chr8.3:17780522-17780541 | None:intergenic | 35.0% | |
| TAGACAAAATCAGTGGAACA+TGG | + | chr8.3:17780450-17780469 | MS.gene48315:CDS | 35.0% | |
| TCCAGATAACAGAACTACAT+AGG | - | chr8.3:17780429-17780448 | None:intergenic | 35.0% | |
| TGCATTTGATTCCAACGATA+AGG | + | chr8.3:17780721-17780740 | MS.gene48315:CDS | 35.0% | |
| ! | ACCTATGTAGTTCTGTTATC+TGG | + | chr8.3:17780425-17780444 | MS.gene48315:intron | 35.0% |
| ! | TGCATTGATCTCAAACAAGT+TGG | - | chr8.3:17780786-17780805 | None:intergenic | 35.0% |
| ! | TTCTTTGAAAAGAACCCTTG+AGG | + | chr8.3:17779841-17779860 | MS.gene48315:CDS | 35.0% |
| ! | TTTGAGATCAATGCAATCAG+TGG | + | chr8.3:17780791-17780810 | MS.gene48315:CDS | 35.0% |
| !! | GTCCAGTGTGTTATTTAAGA+AGG | + | chr8.3:17780187-17780206 | MS.gene48315:intron | 35.0% |
| !!! | GTTTTGTTGGAAGCATTGTA+GGG | - | chr8.3:17780635-17780654 | None:intergenic | 35.0% |
| AACGATAAGGAAGTGGCTTA+TGG | + | chr8.3:17780734-17780753 | MS.gene48315:CDS | 40.0% | |
| ACTTACCTTGTCCATGCTTA+GGG | - | chr8.3:17779877-17779896 | None:intergenic | 40.0% | |
| ATAAGCCACTTCCTTATCGT+TGG | - | chr8.3:17780735-17780754 | None:intergenic | 40.0% | |
| ATGAAGAATCTGTTCCAGCT+GGG | - | chr8.3:17780493-17780512 | None:intergenic | 40.0% | |
| TACTTACCTTGTCCATGCTT+AGG | - | chr8.3:17779878-17779897 | None:intergenic | 40.0% | |
| TATGAAGAATCTGTTCCAGC+TGG | - | chr8.3:17780494-17780513 | None:intergenic | 40.0% | |
| TGATTCCAACGATAAGGAAG+TGG | + | chr8.3:17780727-17780746 | MS.gene48315:CDS | 40.0% | |
| ! | GCATGGCTAACATTTTGTGT+TGG | - | chr8.3:17780613-17780632 | None:intergenic | 40.0% |
| ! | TCTGGAGTAGACAAAATCAG+TGG | + | chr8.3:17780443-17780462 | MS.gene48315:CDS | 40.0% |
| !!! | CGTTTTGTTGGAAGCATTGT+AGG | - | chr8.3:17780636-17780655 | None:intergenic | 40.0% |
| CGTTGTCTTCAGTGCTTTCT+CGG | + | chr8.3:17780841-17780860 | MS.gene48315:CDS | 45.0% | |
| CTTACCTTGTCCATGCTTAG+GGG | - | chr8.3:17779876-17779895 | None:intergenic | 45.0% | |
| GAATCAACCACTCGAAGGTT+TGG | - | chr8.3:17780661-17780680 | None:intergenic | 45.0% | |
| ! | GAAGGTTTGGACCGTTTTGT+TGG | - | chr8.3:17780648-17780667 | None:intergenic | 45.0% |
| !! | CGAGAAAGCACTGAAGACAA+CGG | - | chr8.3:17780843-17780862 | None:intergenic | 45.0% |
| AAACGGTCCAAACCTTCGAG+TGG | + | chr8.3:17780651-17780670 | MS.gene48315:CDS | 50.0% | |
| AGAATCTGTTCCAGCTGGGA+AGG | - | chr8.3:17780489-17780508 | None:intergenic | 50.0% | |
| AGACCGAGGATGAACTCTCT+AGG | + | chr8.3:17780576-17780595 | MS.gene48315:CDS | 50.0% | |
| AGATATCAAGAGTCGCGTGG+CGG | - | chr8.3:17780817-17780836 | None:intergenic | 50.0% | |
| AGCAGATATCAAGAGTCGCG+TGG | - | chr8.3:17780820-17780839 | None:intergenic | 50.0% | |
| CGTACAAAGTACGTTGCCTG+AGG | - | chr8.3:17780695-17780714 | None:intergenic | 50.0% | |
| CTAAACAAGACCTTCCCAGC+TGG | + | chr8.3:17780476-17780495 | MS.gene48315:CDS | 50.0% | |
| GACCGAGGATGAACTCTCTA+GGG | + | chr8.3:17780577-17780596 | MS.gene48315:CDS | 50.0% | |
| GTCCCTAGAGAGTTCATCCT+CGG | - | chr8.3:17780582-17780601 | None:intergenic | 50.0% | |
| TAAGCCAAAAAGACCGCGCA+TGG | + | chr8.3:17780549-17780568 | MS.gene48315:CDS | 50.0% | |
| TTATGGCCAAACCACGAACG+CGG | + | chr8.3:17780751-17780770 | MS.gene48315:CDS | 50.0% | |
| ! | TTCTCCATGCGCGGTCTTTT+TGG | - | chr8.3:17780556-17780575 | None:intergenic | 50.0% |
| !! | GTTGGAAGCATTGTAGGGCA+TGG | - | chr8.3:17780630-17780649 | None:intergenic | 50.0% |
| !!! | GCGCGGTCTTTTTGGCTTAT+GGG | - | chr8.3:17780548-17780567 | None:intergenic | 50.0% |
| !!! | TGCGCGGTCTTTTTGGCTTA+TGG | - | chr8.3:17780549-17780568 | None:intergenic | 50.0% |
| AGGGGAAGCTGTGACCTCAA+GGG | - | chr8.3:17779858-17779877 | None:intergenic | 55.0% | |
| GCGCTGAATCAACCACTCGA+AGG | - | chr8.3:17780666-17780685 | None:intergenic | 55.0% | |
| GCTTCCCCTAAGCATGGACA+AGG | + | chr8.3:17779869-17779888 | MS.gene48315:CDS | 55.0% | |
| GCTTTCTCGGTTGTGTCCCT+AGG | + | chr8.3:17780854-17780873 | MS.gene48315:CDS | 55.0% | |
| GTCACAGCTTCCCCTAAGCA+TGG | + | chr8.3:17779863-17779882 | MS.gene48315:CDS | 55.0% | |
| TAGGGGAAGCTGTGACCTCA+AGG | - | chr8.3:17779859-17779878 | None:intergenic | 55.0% | |
| TATGGCCAAACCACGAACGC+GGG | + | chr8.3:17780752-17780771 | MS.gene48315:CDS | 55.0% | |
| TCTTACCCGCGTTCGTGGTT+TGG | - | chr8.3:17780760-17780779 | None:intergenic | 55.0% | |
| CCTCGGTCTTTCTCCATGCG+CGG | - | chr8.3:17780565-17780584 | None:intergenic | 60.0% | |
| GGTACTCTTACCCGCGTTCG+TGG | - | chr8.3:17780765-17780784 | None:intergenic | 60.0% | |
| CCGCGCATGGAGAAAGACCG+AGG | + | chr8.3:17780562-17780581 | MS.gene48315:CDS | 65.0% | |
| GATTCAGCGCGACGTGCCTC+AGG | + | chr8.3:17780676-17780695 | MS.gene48315:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 17779761 | 17780925 | 17779761 | ID=MS.gene48315 |
| chr8.3 | mRNA | 17779761 | 17780925 | 17779761 | ID=MS.gene48315.t1;Parent=MS.gene48315 |
| chr8.3 | exon | 17779761 | 17779890 | 17779761 | ID=MS.gene48315.t1.exon1;Parent=MS.gene48315.t1 |
| chr8.3 | CDS | 17779761 | 17779890 | 17779761 | ID=cds.MS.gene48315.t1;Parent=MS.gene48315.t1 |
| chr8.3 | exon | 17780435 | 17780925 | 17780435 | ID=MS.gene48315.t1.exon2;Parent=MS.gene48315.t1 |
| chr8.3 | CDS | 17780435 | 17780925 | 17780435 | ID=cds.MS.gene48315.t1;Parent=MS.gene48315.t1 |
| Gene Sequence |
| Protein sequence |
