AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059944


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059944.t1 MTR_4g104730 95.122 205 10 0 1 205 1 205 1.24e-148 411
MS.gene059944.t1 MTR_4g104700 95.610 205 9 0 1 205 1 205 3.84e-140 390
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059944.t1 AT5G50200 51.174 213 93 5 1 206 1 209 5.38e-67 205
MS.gene059944.t1 AT5G50200 51.174 213 93 5 1 206 1 209 5.38e-67 205
MS.gene059944.t1 AT5G50200 51.174 213 93 5 1 206 1 209 5.38e-67 205
MS.gene059944.t1 AT4G24715 49.524 210 98 5 1 205 23 229 1.85e-64 199

Find 54 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGCATAACTTCAGCTTTA+TGG 0.105787 8.1:-19811561 None:intergenic
CATTGTCTTCAGTGCTTTCT+CGG 0.283646 8.1:+19811883 MS.gene059944:CDS
ATCTATGCAGTTATGTTATC+TGG 0.287035 8.1:+19811467 MS.gene059944:intron
CTCAAATTCAATCACTGCTT+TGG 0.355774 8.1:-19811957 None:intergenic
TACTTACCTTGTCCATGCTT+AGG 0.361592 8.1:-19811345 None:intergenic
ACTTACCTTGTCCATGCTTA+GGG 0.362581 8.1:-19811344 None:intergenic
TTTGAGATCAATGCAATTAG+TGG 0.365486 8.1:+19811833 MS.gene059944:CDS
TCTTACCCGCGTTCGTGCTT+TGG 0.366181 8.1:-19811799 None:intergenic
TGCGTTTGATTCTAATGATA+AGG 0.378193 8.1:+19811763 MS.gene059944:CDS
TGTTTAGCTGAAATTTGTTA+TGG 0.421382 8.1:+19811273 MS.gene059944:CDS
AATGATAAGGAAGTGGCTTA+TGG 0.427412 8.1:+19811776 MS.gene059944:CDS
GCTTTCTCGGTTGTGTCCCT+AGG 0.448319 8.1:+19811896 MS.gene059944:CDS
GATTCAGCGCGATGTGCCTC+AGG 0.452907 8.1:+19811718 MS.gene059944:CDS
TAGGATGAGTCTGTTCCAGC+TGG 0.461830 8.1:-19811533 None:intergenic
GTCCCGGGAGAGTTCATCCT+CGG 0.470868 8.1:-19811621 None:intergenic
AGCTGAAATTTGTTATGGAA+AGG 0.496497 8.1:+19811278 MS.gene059944:CDS
CTTCTATATTGAAAAGAGGA+AGG 0.500127 8.1:+19811925 MS.gene059944:CDS
TGCATTGATCTCAAACAAGT+TGG 0.505667 8.1:-19811825 None:intergenic
TGTTCTTCTATATTGAAAAG+AGG 0.523926 8.1:+19811921 MS.gene059944:CDS
TAGGGGAAGCTGTGACCTCA+AGG 0.526901 8.1:-19811326 None:intergenic
TGATTCTAATGATAAGGAAG+TGG 0.527081 8.1:+19811769 MS.gene059944:CDS
GTACAATGCTTCCAACAAAA+CGG 0.527554 8.1:+19811676 MS.gene059944:CDS
AGACCGAGGATGAACTCTCC+CGG 0.533219 8.1:+19811618 MS.gene059944:CDS
CTAAACAAAACTTTCCCAGC+TGG 0.548684 8.1:+19811518 MS.gene059944:CDS
TAGACAAAATCAGTGGGACA+TGG 0.555670 8.1:+19811492 MS.gene059944:CDS
TTCTATATTGAAAAGAGGAA+GGG 0.562873 8.1:+19811926 MS.gene059944:CDS
GTTGGAAGCATTGTACGGCA+TGG 0.569636 8.1:-19811669 None:intergenic
AACTTGCCAACACAAAATGT+TGG 0.571501 8.1:+19811646 MS.gene059944:CDS
GACCGAGGATGAACTCTCCC+GGG 0.572562 8.1:+19811619 MS.gene059944:CDS
CGAGAAAGCACTGAAGACAA+TGG 0.583492 8.1:-19811882 None:intergenic
GTCACAGCTTCCCCTAAGCA+TGG 0.584742 8.1:+19811333 MS.gene059944:CDS
CAATATAGAAGAACACACCT+AGG 0.601286 8.1:-19811913 None:intergenic
CTTACCTTGTCCATGCTTAG+GGG 0.615805 8.1:-19811343 None:intergenic
AGACAAAATCAGTGGGACAT+GGG 0.616176 8.1:+19811493 MS.gene059944:CDS
TATGGCAGCACACAAGCTTG+TGG 0.620979 8.1:+19811230 None:intergenic
TTCTTTGAAAAGAACCCTTG+AGG 0.622622 8.1:+19811311 MS.gene059944:CDS
ATCATTAGAATCAAACGCAT+AGG 0.624906 8.1:-19811759 None:intergenic
AGGGGAAGCTGTGACCTCAA+GGG 0.635053 8.1:-19811325 None:intergenic
TTATGGCCAAAGCACGAACG+CGG 0.635304 8.1:+19811793 MS.gene059944:CDS
CTGGAGTAGACAAAATCAGT+GGG 0.640296 8.1:+19811486 MS.gene059944:CDS
AGGATGAGTCTGTTCCAGCT+GGG 0.643710 8.1:-19811532 None:intergenic
TCTGGAGTAGACAAAATCAG+TGG 0.645792 8.1:+19811485 MS.gene059944:CDS
GCGCTGAATCAACCACTCGA+AGG 0.649799 8.1:-19811705 None:intergenic
CCTCGGTCTTTCTCCAAGCG+CGG 0.651070 8.1:-19811604 None:intergenic
AATATAGAAGAACACACCTA+GGG 0.653049 8.1:-19811912 None:intergenic
TATGGCCAAAGCACGAACGC+GGG 0.662298 8.1:+19811794 MS.gene059944:CDS
GCTTCCCCTAAGCATGGACA+AGG 0.668529 8.1:+19811339 MS.gene059944:CDS
AGATATCAAGTGTCGCGTGG+CGG 0.669328 8.1:-19811856 None:intergenic
AGCAGATATCAAGTGTCGCG+TGG 0.669511 8.1:-19811859 None:intergenic
CGTACAAAGTACGTGGCCTG+AGG 0.676173 8.1:-19811734 None:intergenic
AAACGGTGCAAACCTTCGAG+TGG 0.691223 8.1:+19811693 MS.gene059944:CDS
CCGCGCTTGGAGAAAGACCG+AGG 0.703551 8.1:+19811604 MS.gene059944:CDS
ATAGGCACGTACAAAGTACG+TGG 0.771630 8.1:-19811741 None:intergenic
AAAGCTGAAGTTATGCTACG+CGG 0.775950 8.1:+19811565 MS.gene059944:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATTGAGTTTATATTGA+AGG - chr8.1:19811438-19811457 None:intergenic 20.0%
! TAATTAAATACGCCAATACA+AGG - chr8.1:19811387-19811406 None:intergenic 25.0%
! TGTTCTTCTATATTGAAAAG+AGG + chr8.1:19811921-19811940 MS.gene059944:CDS 25.0%
! TTCTATATTGAAAAGAGGAA+GGG + chr8.1:19811926-19811945 MS.gene059944:CDS 25.0%
!!! TGTTTAGCTGAAATTTGTTA+TGG + chr8.1:19811273-19811292 MS.gene059944:CDS 25.0%
AATATAGAAGAACACACCTA+GGG - chr8.1:19811915-19811934 None:intergenic 30.0%
ATCATTAGAATCAAACGCAT+AGG - chr8.1:19811762-19811781 None:intergenic 30.0%
ATCTATGCAGTTATGTTATC+TGG + chr8.1:19811467-19811486 MS.gene059944:intron 30.0%
CTTCTATATTGAAAAGAGGA+AGG + chr8.1:19811925-19811944 MS.gene059944:CDS 30.0%
TGATTCTAATGATAAGGAAG+TGG + chr8.1:19811769-19811788 MS.gene059944:CDS 30.0%
TGCGTTTGATTCTAATGATA+AGG + chr8.1:19811763-19811782 MS.gene059944:CDS 30.0%
! TAAGATTTTGCACCTTGTAT+TGG + chr8.1:19811372-19811391 MS.gene059944:intron 30.0%
! TTTGAGATCAATGCAATTAG+TGG + chr8.1:19811833-19811852 MS.gene059944:CDS 30.0%
!! AGCTGAAATTTGTTATGGAA+AGG + chr8.1:19811278-19811297 MS.gene059944:CDS 30.0%
AACTTGCCAACACAAAATGT+TGG + chr8.1:19811646-19811665 MS.gene059944:CDS 35.0%
AATGATAAGGAAGTGGCTTA+TGG + chr8.1:19811776-19811795 MS.gene059944:CDS 35.0%
CAATATAGAAGAACACACCT+AGG - chr8.1:19811916-19811935 None:intergenic 35.0%
CTCAAATTCAATCACTGCTT+TGG - chr8.1:19811960-19811979 None:intergenic 35.0%
GTACAATGCTTCCAACAAAA+CGG + chr8.1:19811676-19811695 MS.gene059944:CDS 35.0%
GTAGCATAACTTCAGCTTTA+TGG - chr8.1:19811564-19811583 None:intergenic 35.0%
! TGCATTGATCTCAAACAAGT+TGG - chr8.1:19811828-19811847 None:intergenic 35.0%
! TTCTTTGAAAAGAACCCTTG+AGG + chr8.1:19811311-19811330 MS.gene059944:CDS 35.0%
!!! CTTCAGCTTTATGGTTTTGT+AGG - chr8.1:19811555-19811574 None:intergenic 35.0%
!!! GTTTTGTTGGAAGCATTGTA+CGG - chr8.1:19811677-19811696 None:intergenic 35.0%
AAAGCTGAAGTTATGCTACG+CGG + chr8.1:19811565-19811584 MS.gene059944:CDS 40.0%
ACTTACCTTGTCCATGCTTA+GGG - chr8.1:19811347-19811366 None:intergenic 40.0%
AGACAAAATCAGTGGGACAT+GGG + chr8.1:19811493-19811512 MS.gene059944:CDS 40.0%
CATTGTCTTCAGTGCTTTCT+CGG + chr8.1:19811883-19811902 MS.gene059944:CDS 40.0%
CTAAACAAAACTTTCCCAGC+TGG + chr8.1:19811518-19811537 MS.gene059944:CDS 40.0%
CTGGAGTAGACAAAATCAGT+GGG + chr8.1:19811486-19811505 MS.gene059944:CDS 40.0%
TACTTACCTTGTCCATGCTT+AGG - chr8.1:19811348-19811367 None:intergenic 40.0%
TAGACAAAATCAGTGGGACA+TGG + chr8.1:19811492-19811511 MS.gene059944:CDS 40.0%
! TCTGGAGTAGACAAAATCAG+TGG + chr8.1:19811485-19811504 MS.gene059944:CDS 40.0%
ATAGGCACGTACAAAGTACG+TGG - chr8.1:19811744-19811763 None:intergenic 45.0%
CTTACCTTGTCCATGCTTAG+GGG - chr8.1:19811346-19811365 None:intergenic 45.0%
! GAAGGTTTGCACCGTTTTGT+TGG - chr8.1:19811690-19811709 None:intergenic 45.0%
! GCATGGCCAACATTTTGTGT+TGG - chr8.1:19811655-19811674 None:intergenic 45.0%
!! CGAGAAAGCACTGAAGACAA+TGG - chr8.1:19811885-19811904 None:intergenic 45.0%
!! TGTGTTGGCAAGTTTTGTCC+CGG - chr8.1:19811640-19811659 None:intergenic 45.0%
AAACGGTGCAAACCTTCGAG+TGG + chr8.1:19811693-19811712 MS.gene059944:CDS 50.0%
AGGATGAGTCTGTTCCAGCT+GGG - chr8.1:19811535-19811554 None:intergenic 50.0%
TAAGCCAAAAAGACCGCGCT+TGG + chr8.1:19811591-19811610 MS.gene059944:CDS 50.0%
TAGGATGAGTCTGTTCCAGC+TGG - chr8.1:19811536-19811555 None:intergenic 50.0%
TTATGGCCAAAGCACGAACG+CGG + chr8.1:19811793-19811812 MS.gene059944:CDS 50.0%
! AGATATCAAGTGTCGCGTGG+CGG - chr8.1:19811859-19811878 None:intergenic 50.0%
! AGCAGATATCAAGTGTCGCG+TGG - chr8.1:19811862-19811881 None:intergenic 50.0%
! TTCTCCAAGCGCGGTCTTTT+TGG - chr8.1:19811598-19811617 None:intergenic 50.0%
!! GTGTTGGCAAGTTTTGTCCC+GGG - chr8.1:19811639-19811658 None:intergenic 50.0%
!! GTTGGAAGCATTGTACGGCA+TGG - chr8.1:19811672-19811691 None:intergenic 50.0%
!!! AGCGCGGTCTTTTTGGCTTA+TGG - chr8.1:19811591-19811610 None:intergenic 50.0%
AGACCGAGGATGAACTCTCC+CGG + chr8.1:19811618-19811637 MS.gene059944:CDS 55.0%
AGGGGAAGCTGTGACCTCAA+GGG - chr8.1:19811328-19811347 None:intergenic 55.0%
CGTACAAAGTACGTGGCCTG+AGG - chr8.1:19811737-19811756 None:intergenic 55.0%
GCGCTGAATCAACCACTCGA+AGG - chr8.1:19811708-19811727 None:intergenic 55.0%
GCTTCCCCTAAGCATGGACA+AGG + chr8.1:19811339-19811358 MS.gene059944:CDS 55.0%
GCTTTCTCGGTTGTGTCCCT+AGG + chr8.1:19811896-19811915 MS.gene059944:CDS 55.0%
GTCACAGCTTCCCCTAAGCA+TGG + chr8.1:19811333-19811352 MS.gene059944:CDS 55.0%
TAGGGGAAGCTGTGACCTCA+AGG - chr8.1:19811329-19811348 None:intergenic 55.0%
TATGGCCAAAGCACGAACGC+GGG + chr8.1:19811794-19811813 MS.gene059944:CDS 55.0%
TCTTACCCGCGTTCGTGCTT+TGG - chr8.1:19811802-19811821 None:intergenic 55.0%
CCTCGGTCTTTCTCCAAGCG+CGG - chr8.1:19811607-19811626 None:intergenic 60.0%
GACCGAGGATGAACTCTCCC+GGG + chr8.1:19811619-19811638 MS.gene059944:CDS 60.0%
GATTCAGCGCGATGTGCCTC+AGG + chr8.1:19811718-19811737 MS.gene059944:CDS 60.0%
GTCCCGGGAGAGTTCATCCT+CGG - chr8.1:19811624-19811643 None:intergenic 60.0%
CCGCGCTTGGAGAAAGACCG+AGG + chr8.1:19811604-19811623 MS.gene059944:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 19811231 19811982 19811231 ID=MS.gene059944
chr8.1 mRNA 19811231 19811982 19811231 ID=MS.gene059944.t1;Parent=MS.gene059944
chr8.1 exon 19811231 19811360 19811231 ID=MS.gene059944.t1.exon1;Parent=MS.gene059944.t1
chr8.1 CDS 19811231 19811360 19811231 ID=cds.MS.gene059944.t1;Parent=MS.gene059944.t1
chr8.1 exon 19811477 19811982 19811477 ID=MS.gene059944.t1.exon2;Parent=MS.gene059944.t1
chr8.1 CDS 19811477 19811982 19811477 ID=cds.MS.gene059944.t1;Parent=MS.gene059944.t1
Gene Sequence

>MS.gene059944

ATGGCAGCACACAAGCTTGTGGCAGCATCACTTCTTCTATGCTGTTTAGCTGAAATTTGTTATGGAAAGGATCTCTTTTCTTCTTTGAAAAGAACCCTTGAGGTCACAGCTTCCCCTAAGCATGGACAAGGTAAGTAACAATAAGATTTTGCACCTTGTATTGGCGTATTTAATTATTTTATAAAAGTCATGATGTTAAATACTCCTTCAATATAAACTCAATTTTGCTATGAAATATCTATGCAGTTATGTTATCTGGAGTAGACAAAATCAGTGGGACATGGGCTCTAAACAAAACTTTCCCAGCTGGAACAGACTCATCCTACAAAACCATAAAGCTGAAGTTATGCTACGCGGCCATAAGCCAAAAAGACCGCGCTTGGAGAAAGACCGAGGATGAACTCTCCCGGGACAAAACTTGCCAACACAAAATGTTGGCCATGCCGTACAATGCTTCCAACAAAACGGTGCAAACCTTCGAGTGGTTGATTCAGCGCGATGTGCCTCAGGCCACGTACTTTGTACGTGCCTATGCGTTTGATTCTAATGATAAGGAAGTGGCTTATGGCCAAAGCACGAACGCGGGTAAGAGTACCAACTTGTTTGAGATCAATGCAATTAGTGGCCGCCACGCGACACTTGATATCTGCTCCATTGTCTTCAGTGCTTTCTCGGTTGTGTCCCTAGGTGTGTTCTTCTATATTGAAAAGAGGAAGGGAAAAGTCACCAAAGCAGTGATTGAATTTGAGTGA

Protein sequence

>MS.gene059944.t1

MAAHKLVAASLLLCCLAEICYGKDLFSSLKRTLEVTASPKHGQVMLSGVDKISGTWALNKTFPAGTDSSYKTIKLKLCYAAISQKDRAWRKTEDELSRDKTCQHKMLAMPYNASNKTVQTFEWLIQRDVPQATYFVRAYAFDSNDKEVAYGQSTNAGKSTNLFEINAISGRHATLDICSIVFSAFSVVSLGVFFYIEKRKGKVTKAVIEFE