Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49116.t1 | XP_003592254.1 | 91.6 | 179 | 10 | 2 | 1 | 178 | 1 | 175 | 5.70E-82 | 313.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49116.t1 | G7IES2 | 91.6 | 179 | 10 | 2 | 1 | 178 | 1 | 175 | 4.1e-82 | 313.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052340 | MS.gene49116 | 0.817281 | 3.58E-52 | -1.69E-46 |
| MS.gene057396 | MS.gene49116 | 0.804252 | 2.36E-49 | -1.69E-46 |
| MS.gene057927 | MS.gene49116 | 0.801143 | 1.03E-48 | -1.69E-46 |
| MS.gene058365 | MS.gene49116 | 0.810271 | 1.25E-50 | -1.69E-46 |
| MS.gene059788 | MS.gene49116 | 0.819139 | 1.36E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49116.t1 | MTR_1g100800 | 91.620 | 179 | 10 | 2 | 1 | 178 | 1 | 175 | 5.47e-103 | 298 |
| MS.gene49116.t1 | MTR_8g094540 | 38.583 | 127 | 77 | 1 | 53 | 178 | 34 | 160 | 2.18e-23 | 94.7 |
| MS.gene49116.t1 | MTR_3g096890 | 35.762 | 151 | 88 | 4 | 36 | 178 | 22 | 171 | 4.15e-21 | 88.6 |
| MS.gene49116.t1 | MTR_3g096890 | 37.879 | 132 | 75 | 3 | 53 | 178 | 105 | 235 | 3.55e-20 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49116.t1 | AT5G52420 | 49.444 | 180 | 80 | 3 | 1 | 178 | 1 | 171 | 1.23e-53 | 172 |
| MS.gene49116.t1 | AT1G27990 | 42.188 | 128 | 72 | 2 | 53 | 178 | 47 | 174 | 2.24e-26 | 102 |
| MS.gene49116.t1 | AT5G23920 | 40.310 | 129 | 75 | 2 | 52 | 178 | 31 | 159 | 6.34e-25 | 98.2 |
Find 22 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGAAAACATAAAGTTTA+AGG | 0.192623 | 1.3:+68997047 | None:intergenic |
| TCAATTTCTTGTTCTTCTTT+TGG | 0.274467 | 1.3:+68997331 | None:intergenic |
| ACAGGAACAAAAGCTCTTAT+TGG | 0.282027 | 1.3:+68996869 | None:intergenic |
| ATTACAAATCCAAAATCTTC+AGG | 0.292734 | 1.3:+68997163 | None:intergenic |
| ATGGTTAGCCCTGAAGATTT+TGG | 0.385009 | 1.3:-68997172 | MS.gene49116:CDS |
| TTTGCCTTCTTTCGTTTAGC+TGG | 0.391031 | 1.3:+68996798 | None:intergenic |
| TTTGTTCCTGTTCTTTATGT+GGG | 0.396871 | 1.3:-68996857 | MS.gene49116:CDS |
| CAGCTAAACAATTGAGTTGT+CGG | 0.396906 | 1.3:+68997221 | None:intergenic |
| TGGAATATTTGAAGGTGATA+AGG | 0.427078 | 1.3:-68996981 | MS.gene49116:CDS |
| TCTGGTGGAGTTGGACCAAC+TGG | 0.442420 | 1.3:-68997370 | MS.gene49116:CDS |
| ATTCTTCAACTGTGCATTCA+CGG | 0.445823 | 1.3:+68996750 | None:intergenic |
| CCATGTAAAATATATGCTAT+AGG | 0.465903 | 1.3:+68997001 | None:intergenic |
| TTACAAATCCAAAATCTTCA+GGG | 0.499930 | 1.3:+68997164 | None:intergenic |
| CTGGACCCCACATAAAGAAC+AGG | 0.505546 | 1.3:+68996851 | None:intergenic |
| AATTGAGTTGTCGGAATGAG+AGG | 0.532641 | 1.3:+68997230 | None:intergenic |
| TTCAGGGCTAACCATGCCAC+TGG | 0.540431 | 1.3:+68997180 | None:intergenic |
| TTGTTCCTGTTCTTTATGTG+GGG | 0.541233 | 1.3:-68996856 | MS.gene49116:CDS |
| TGTGCCAGCTAAACGAAAGA+AGG | 0.557291 | 1.3:-68996802 | MS.gene49116:CDS |
| TGTCTTATCAGCCAGTGGCA+TGG | 0.570622 | 1.3:-68997191 | MS.gene49116:CDS |
| GTTGTTGTCTTATCAGCCAG+TGG | 0.595119 | 1.3:-68997196 | MS.gene49116:CDS |
| AGGCTAATGTCACAGCCAGT+TGG | 0.608232 | 1.3:+68997355 | None:intergenic |
| ATATTTGAAGGTGATAAGGA+AGG | 0.616957 | 1.3:-68996977 | MS.gene49116:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATGAAAACATAAAGTTTA+AGG | + | chr1.3:68997074-68997093 | None:intergenic | 20.0% |
| !!! | ATTTTACATGGAATATTTGA+AGG | - | chr1.3:68997129-68997148 | MS.gene49116:CDS | 20.0% |
| !!! | CTTAAACTTTATGTTTTCAT+TGG | - | chr1.3:68997072-68997091 | MS.gene49116:CDS | 20.0% |
| ! | ATTACAAATCCAAAATCTTC+AGG | + | chr1.3:68996958-68996977 | None:intergenic | 25.0% |
| ! | CCATGTAAAATATATGCTAT+AGG | + | chr1.3:68997120-68997139 | None:intergenic | 25.0% |
| ! | TTACAAATCCAAAATCTTCA+GGG | + | chr1.3:68996957-68996976 | None:intergenic | 25.0% |
| !! | CCTATAGCATATATTTTACA+TGG | - | chr1.3:68997117-68997136 | MS.gene49116:CDS | 25.0% |
| !! | TCAATTTCTTGTTCTTCTTT+TGG | + | chr1.3:68996790-68996809 | None:intergenic | 25.0% |
| !!! | TTGTTTTGTTGAGTGAAAAT+TGG | + | chr1.3:68997048-68997067 | None:intergenic | 25.0% |
| ATATTTGAAGGTGATAAGGA+AGG | - | chr1.3:68997141-68997160 | MS.gene49116:CDS | 30.0% | |
| TGGAATATTTGAAGGTGATA+AGG | - | chr1.3:68997137-68997156 | MS.gene49116:CDS | 30.0% | |
| TTTGTTCCTGTTCTTTATGT+GGG | - | chr1.3:68997261-68997280 | MS.gene49116:CDS | 30.0% | |
| ! | TACACCACATGTTTTTCTTT+TGG | - | chr1.3:68997176-68997195 | MS.gene49116:CDS | 30.0% |
| ! | TTTCTTGTTCTTCTTTTGGT+AGG | + | chr1.3:68996786-68996805 | None:intergenic | 30.0% |
| ! | TTTTGTTCCTGTTCTTTATG+TGG | - | chr1.3:68997260-68997279 | MS.gene49116:CDS | 30.0% |
| !! | TTTGGCTAGTCAAGTTTTTA+TGG | - | chr1.3:68997194-68997213 | MS.gene49116:CDS | 30.0% |
| !!! | AATTGTTGTTGTTTTGCACA+TGG | + | chr1.3:68997293-68997312 | None:intergenic | 30.0% |
| !!! | AGGAAACTAGTTTTTCTTTG+TGG | + | chr1.3:68996871-68996890 | None:intergenic | 30.0% |
| ACAGGAACAAAAGCTCTTAT+TGG | + | chr1.3:68997252-68997271 | None:intergenic | 35.0% | |
| ATTCTTCAACTGTGCATTCA+CGG | + | chr1.3:68997371-68997390 | None:intergenic | 35.0% | |
| CTAGCCAAAAGAAAAACATG+TGG | + | chr1.3:68997183-68997202 | None:intergenic | 35.0% | |
| TTGTTCCTGTTCTTTATGTG+GGG | - | chr1.3:68997262-68997281 | MS.gene49116:CDS | 35.0% | |
| ! | CAGCTAAACAATTGAGTTGT+CGG | + | chr1.3:68996900-68996919 | None:intergenic | 35.0% |
| ! | GCTAGTCAAGTTTTTATGGA+AGG | - | chr1.3:68997198-68997217 | MS.gene49116:CDS | 35.0% |
| ! | GTTTTCATTGGAGCAATCAT+TGG | - | chr1.3:68997084-68997103 | MS.gene49116:CDS | 35.0% |
| !! | TGTTCTTTTGAAGAGTGAGA+TGG | + | chr1.3:68997024-68997043 | None:intergenic | 35.0% |
| !!! | ACTAGTTTTTCTTTGTGGTG+TGG | + | chr1.3:68996866-68996885 | None:intergenic | 35.0% |
| !!! | GAAGGTGTTGCATTTTCAAA+TGG | - | chr1.3:68997216-68997235 | MS.gene49116:CDS | 35.0% |
| TTTGCCTTCTTTCGTTTAGC+TGG | + | chr1.3:68997323-68997342 | None:intergenic | 40.0% | |
| ! | AATTGAGTTGTCGGAATGAG+AGG | + | chr1.3:68996891-68996910 | None:intergenic | 40.0% |
| !! | ATGGTTAGCCCTGAAGATTT+TGG | - | chr1.3:68996946-68996965 | MS.gene49116:CDS | 40.0% |
| GTTGTTGTCTTATCAGCCAG+TGG | - | chr1.3:68996922-68996941 | MS.gene49116:CDS | 45.0% | |
| TGTGCCAGCTAAACGAAAGA+AGG | - | chr1.3:68997316-68997335 | MS.gene49116:CDS | 45.0% | |
| !!! | GTTGTTGTTTTGCACATGGC+TGG | + | chr1.3:68997289-68997308 | None:intergenic | 45.0% |
| AGGCTAATGTCACAGCCAGT+TGG | + | chr1.3:68996766-68996785 | None:intergenic | 50.0% | |
| CTGGACCCCACATAAAGAAC+AGG | + | chr1.3:68997270-68997289 | None:intergenic | 50.0% | |
| TGTCTTATCAGCCAGTGGCA+TGG | - | chr1.3:68996927-68996946 | MS.gene49116:CDS | 50.0% | |
| TCTGGTGGAGTTGGACCAAC+TGG | - | chr1.3:68996748-68996767 | MS.gene49116:CDS | 55.0% | |
| TTCAGGGCTAACCATGCCAC+TGG | + | chr1.3:68996941-68996960 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 68996745 | 68997395 | 68996745 | ID=MS.gene49116 |
| chr1.3 | mRNA | 68996745 | 68997395 | 68996745 | ID=MS.gene49116.t1;Parent=MS.gene49116 |
| chr1.3 | exon | 68996745 | 68997395 | 68996745 | ID=MS.gene49116.t1.exon1;Parent=MS.gene49116.t1 |
| chr1.3 | CDS | 68996745 | 68997395 | 68996745 | ID=cds.MS.gene49116.t1;Parent=MS.gene49116.t1 |
| Gene Sequence |
| Protein sequence |
