Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49184.t1 | RHN81829.1 | 98.2 | 55 | 0 | 1 | 1 | 54 | 811 | 865 | 3.10E-21 | 110.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49184.t1 | A0A072VNW0 | 98.2 | 55 | 0 | 1 | 1 | 54 | 775 | 829 | 2.3e-21 | 110.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049801 | MS.gene49184 | 0.80476 | 1.85E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene49184 | 0.802403 | 5.70E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene49184 | 0.811772 | 5.92E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene49184 | 0.801701 | 7.95E-49 | -1.69E-46 |
| MS.gene06010 | MS.gene49184 | 0.846616 | 1.94E-59 | -1.69E-46 |
| MS.gene060869 | MS.gene49184 | 0.82935 | 5.41E-55 | -1.69E-46 |
| MS.gene060870 | MS.gene49184 | 0.84734 | 1.23E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49184.t1 | MTR_1g100623 | 98.182 | 55 | 0 | 1 | 1 | 54 | 727 | 781 | 2.43e-31 | 113 |
| MS.gene49184.t1 | MTR_1g100623 | 98.182 | 55 | 0 | 1 | 1 | 54 | 775 | 829 | 2.88e-31 | 113 |
| MS.gene49184.t1 | MTR_1g100607 | 84.211 | 57 | 6 | 2 | 1 | 54 | 153 | 209 | 6.07e-25 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAGGGTTAAGGATTATTA+TGG | 0.244321 | 1.1:+72425464 | MS.gene49184:CDS |
| TTAAGGATTATTATGGATCT+TGG | 0.355305 | 1.1:+72425471 | MS.gene49184:CDS |
| AGGTATGGTTGACAGGGTTA+AGG | 0.362709 | 1.1:+72425454 | None:intergenic |
| TGTGGTGATTGATTCTCCTC+AGG | 0.432207 | 1.1:-72425503 | None:intergenic |
| CAGGAAAAGGTATGGTTGAC+AGG | 0.438569 | 1.1:+72425447 | None:intergenic |
| TAAAGATCATGTTGCAGAAG+TGG | 0.479475 | 1.1:+72425648 | MS.gene49184:CDS |
| GTTGGCAAAGGTGTGGATGA+AGG | 0.542986 | 1.1:+72425676 | MS.gene49184:CDS |
| TGAGGAGAATCAATCACCAC+AGG | 0.595498 | 1.1:+72425505 | MS.gene49184:CDS |
| GCAAAGGTGTGGATGAAGGA+AGG | 0.607651 | 1.1:+72425680 | MS.gene49184:CDS |
| GTTGCAGAAGTGGAACAAGT+TGG | 0.612425 | 1.1:+72425658 | MS.gene49184:CDS |
| ATCTTGGTTTGCTAAACCTG+AGG | 0.638772 | 1.1:+72425487 | MS.gene49184:CDS |
| GAAGTGGAACAAGTTGGCAA+AGG | 0.645524 | 1.1:+72425664 | MS.gene49184:CDS |
| GGAACAAGTTGGCAAAGGTG+TGG | 0.655366 | 1.1:+72425669 | MS.gene49184:CDS |
| AGGAAAAGGTATGGTTGACA+GGG | 0.656335 | 1.1:+72425448 | None:intergenic |
| GAGGAGAATCAATCACCACA+GGG | 0.732593 | 1.1:+72425506 | MS.gene49184:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTTGTTATTATACTTAT+AGG | + | chr1.1:72425598-72425617 | MS.gene49184:intron | 15.0% |
| ! | GTTATTATACTTATAGGTGT+AGG | + | chr1.1:72425604-72425623 | MS.gene49184:intron | 25.0% |
| ! | TTAAGGATTATTATGGATCT+TGG | + | chr1.1:72425471-72425490 | MS.gene49184:CDS | 25.0% |
| AGAGAAAAAGAATTACCCTG+TGG | - | chr1.1:72425524-72425543 | None:intergenic | 35.0% | |
| GACAGGGTTAAGGATTATTA+TGG | + | chr1.1:72425464-72425483 | MS.gene49184:CDS | 35.0% | |
| TAAAGATCATGTTGCAGAAG+TGG | + | chr1.1:72425648-72425667 | MS.gene49184:CDS | 35.0% | |
| !! | ATCTTGGTTTGCTAAACCTG+AGG | + | chr1.1:72425487-72425506 | MS.gene49184:CDS | 40.0% |
| GAGGAGAATCAATCACCACA+GGG | + | chr1.1:72425506-72425525 | MS.gene49184:CDS | 45.0% | |
| GTTGCAGAAGTGGAACAAGT+TGG | + | chr1.1:72425658-72425677 | MS.gene49184:CDS | 45.0% | |
| TGAGGAGAATCAATCACCAC+AGG | + | chr1.1:72425505-72425524 | MS.gene49184:CDS | 45.0% | |
| ! | GAAGTGGAACAAGTTGGCAA+AGG | + | chr1.1:72425664-72425683 | MS.gene49184:CDS | 45.0% |
| ! | TGTGGTGATTGATTCTCCTC+AGG | - | chr1.1:72425506-72425525 | None:intergenic | 45.0% |
| ! | GCAAAGGTGTGGATGAAGGA+AGG | + | chr1.1:72425680-72425699 | MS.gene49184:CDS | 50.0% |
| ! | GGAACAAGTTGGCAAAGGTG+TGG | + | chr1.1:72425669-72425688 | MS.gene49184:CDS | 50.0% |
| ! | GTTGGCAAAGGTGTGGATGA+AGG | + | chr1.1:72425676-72425695 | MS.gene49184:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 72425458 | 72425714 | 72425458 | ID=MS.gene49184 |
| chr1.1 | mRNA | 72425458 | 72425714 | 72425458 | ID=MS.gene49184.t1;Parent=MS.gene49184 |
| chr1.1 | exon | 72425458 | 72425527 | 72425458 | ID=MS.gene49184.t1.exon1;Parent=MS.gene49184.t1 |
| chr1.1 | CDS | 72425458 | 72425527 | 72425458 | ID=cds.MS.gene49184.t1;Parent=MS.gene49184.t1 |
| chr1.1 | exon | 72425620 | 72425714 | 72425620 | ID=MS.gene49184.t1.exon2;Parent=MS.gene49184.t1 |
| chr1.1 | CDS | 72425620 | 72425714 | 72425620 | ID=cds.MS.gene49184.t1;Parent=MS.gene49184.t1 |
| Gene Sequence |
| Protein sequence |
