Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49934.t1 | XP_003620535.2 | 95.1 | 325 | 16 | 0 | 232 | 556 | 62 | 386 | 1.10E-185 | 659.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49934.t1 | Q8VXZ0 | 64.0 | 322 | 106 | 3 | 239 | 556 | 59 | 374 | 1.3e-115 | 418.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49934.t1 | G7KNJ9 | 95.1 | 325 | 16 | 0 | 232 | 556 | 62 | 386 | 7.9e-186 | 659.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene49934 | 0.826045 | 3.37E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene49934 | 0.810773 | 9.76E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene49934 | 0.801444 | 8.98E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene49934 | -0.804872 | 1.75E-49 | -1.69E-46 |
| MS.gene052077 | MS.gene49934 | 0.826459 | 2.68E-54 | -1.69E-46 |
| MS.gene052389 | MS.gene49934 | 0.8208 | 5.66E-53 | -1.69E-46 |
| MS.gene052420 | MS.gene49934 | 0.941246 | 5.80E-101 | -1.69E-46 |
| MS.gene053836 | MS.gene49934 | 0.807891 | 4.05E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene49934 | 0.807939 | 3.96E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene49934 | 0.805034 | 1.62E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49934.t1 | MTR_6g086510 | 95.077 | 325 | 16 | 0 | 232 | 556 | 62 | 386 | 0.0 | 654 |
| MS.gene49934.t1 | MTR_6g086560 | 81.550 | 271 | 14 | 2 | 1 | 235 | 9 | 279 | 1.03e-149 | 431 |
| MS.gene49934.t1 | MTR_3g045440 | 50.741 | 270 | 97 | 2 | 1 | 235 | 1 | 269 | 1.37e-87 | 271 |
| MS.gene49934.t1 | MTR_1g018320 | 36.719 | 256 | 124 | 3 | 12 | 231 | 10 | 263 | 2.33e-51 | 177 |
| MS.gene49934.t1 | MTR_5g016150 | 33.071 | 254 | 132 | 4 | 13 | 230 | 9 | 260 | 8.60e-41 | 150 |
| MS.gene49934.t1 | MTR_4g095310 | 32.283 | 254 | 134 | 4 | 13 | 230 | 9 | 260 | 9.39e-41 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49934.t1 | AT5G19460 | 61.584 | 341 | 119 | 4 | 222 | 556 | 40 | 374 | 7.62e-143 | 417 |
| MS.gene49934.t1 | AT5G19470 | 58.929 | 336 | 129 | 4 | 223 | 556 | 37 | 365 | 3.86e-132 | 390 |
| MS.gene49934.t1 | AT5G19470 | 58.413 | 315 | 122 | 4 | 223 | 535 | 37 | 344 | 8.87e-122 | 363 |
| MS.gene49934.t1 | AT3G24420 | 44.906 | 265 | 109 | 3 | 3 | 231 | 5 | 268 | 1.74e-75 | 241 |
| MS.gene49934.t1 | AT3G03990 | 36.226 | 265 | 131 | 4 | 2 | 231 | 1 | 262 | 1.19e-50 | 175 |
| MS.gene49934.t1 | AT4G37470 | 31.890 | 254 | 135 | 4 | 13 | 230 | 9 | 260 | 5.23e-39 | 144 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTGAAAATTATGGATATT+TGG | 0.161860 | 6.4:-59243747 | MS.gene49934:CDS |
| TAGAATCCAACTATGAAAAT+TGG | 0.176582 | 6.4:-59258544 | MS.gene49934:CDS |
| CGTGCTGCAGCTCCTTATTT+TGG | 0.177594 | 6.4:-59245742 | MS.gene49934:CDS |
| TCAAAGGGTCATAAAGATTT+TGG | 0.191944 | 6.4:+59261288 | None:intergenic |
| GGTTGCAAAGTTTGACTTTA+TGG | 0.196450 | 6.4:+59249877 | None:intergenic |
| CTCTTTGAAGCATAGGCTTA+TGG | 0.222290 | 6.4:-59245357 | MS.gene49934:intron |
| AGATGACTTGATCAGTTTAT+TGG | 0.223698 | 6.4:-59261240 | MS.gene49934:CDS |
| CTCCTATTCTTAAGCTTCTT+AGG | 0.225839 | 6.4:+59243720 | None:intergenic |
| ATTTCTGCAACTTGCTTAAC+AGG | 0.232793 | 6.4:+59244311 | None:intergenic |
| TTCACTGATGGTTCATCATT+TGG | 0.234984 | 6.4:+59258491 | None:intergenic |
| TGTACCTTTATGCCAAAATA+AGG | 0.246886 | 6.4:+59245730 | None:intergenic |
| TACATGGAGAAGAAAATTAA+AGG | 0.288066 | 6.4:-59258305 | MS.gene49934:CDS |
| TTGGAAGTAATTGACACTTT+TGG | 0.304639 | 6.4:-59258272 | MS.gene49934:CDS |
| GTCATTGCAAAACACTGTTT+TGG | 0.305563 | 6.4:+59258415 | None:intergenic |
| TCTTTAACAGCACCAGAAAA+AGG | 0.312331 | 6.4:+59261311 | None:intergenic |
| TACATAAGTCCTGAAAATTA+TGG | 0.313397 | 6.4:-59243755 | MS.gene49934:CDS |
| TGTTGGTGCTGTTTCATATA+TGG | 0.322857 | 6.4:-59244546 | MS.gene49934:CDS |
| TGGCTATGTAATCGAGCATT+TGG | 0.324074 | 6.4:-59245954 | MS.gene49934:CDS |
| TCAGCTAGTTGATGTGCTTA+AGG | 0.333933 | 6.4:-59258222 | MS.gene49934:CDS |
| CCCAATGATGGAAGCCCCTT+TGG | 0.334011 | 6.4:-59246034 | MS.gene49934:CDS |
| ATGGAACAGACCAATCAATA+TGG | 0.335818 | 6.4:-59261391 | MS.gene49934:CDS |
| ATGATCACGACAGCACAATT+TGG | 0.337212 | 6.4:+59244263 | None:intergenic |
| ATAGAGTTGTTCTATTTGAT+TGG | 0.343427 | 6.4:-59261334 | MS.gene49934:CDS |
| CTTAAAGTTGTTAACTTTGT+TGG | 0.343903 | 6.4:-59261206 | MS.gene49934:CDS |
| ATGTAGTGAGCTACACTATA+TGG | 0.347743 | 6.4:+59258323 | None:intergenic |
| TTTGTTGGTCATTCCATGTC+TGG | 0.352708 | 6.4:-59261191 | MS.gene49934:intron |
| CAGCTAGTTGATGTGCTTAA+GGG | 0.371050 | 6.4:-59258221 | MS.gene49934:intron |
| TTGGGAGAGGAGCATATTCC+AGG | 0.372454 | 6.4:-59245935 | MS.gene49934:CDS |
| ACCAAAGGGGCTTCCATCAT+TGG | 0.391806 | 6.4:+59246033 | None:intergenic |
| ATGCTTGATCATCTAGTTGC+AGG | 0.394775 | 6.4:-59245246 | MS.gene49934:CDS |
| GATCAGTTTATTGGATCAAA+TGG | 0.398901 | 6.4:-59261231 | MS.gene49934:CDS |
| AGAAATACCCGTGTCGAAAC+AGG | 0.401405 | 6.4:+59244234 | None:intergenic |
| AGTGGCTATGTTGAGATAGA+TGG | 0.405918 | 6.4:-59245324 | MS.gene49934:CDS |
| CATGTTTCTCAATCTGAAGT+TGG | 0.408924 | 6.4:-59249942 | MS.gene49934:CDS |
| GTCATTGAGGGCGACGTTGT+TGG | 0.409677 | 6.4:-59246219 | MS.gene49934:CDS |
| AGAATCCAACTATGAAAATT+GGG | 0.411548 | 6.4:-59258543 | MS.gene49934:CDS |
| TCCTCAGCTGTCTTCAGCAA+TGG | 0.422425 | 6.4:+59245992 | None:intergenic |
| ATTTGGATCCACTACATTTG+TGG | 0.429468 | 6.4:+59258508 | None:intergenic |
| GGCTATGTAATCGAGCATTT+GGG | 0.440338 | 6.4:-59245953 | MS.gene49934:CDS |
| CTTCTGAATGCTAGAATCCT+AGG | 0.449257 | 6.4:-59261449 | MS.gene49934:CDS |
| CGTTCTAATGAAAAGAAAAC+GGG | 0.450446 | 6.4:+59245763 | None:intergenic |
| AGGAGCATATTCCAGGTATA+CGG | 0.452411 | 6.4:-59245928 | MS.gene49934:CDS |
| TTTCTTCCATTTGTCATTGA+GGG | 0.455068 | 6.4:-59246231 | MS.gene49934:CDS |
| AATGCTAGAATCCTAGGAAC+AGG | 0.457236 | 6.4:-59261443 | MS.gene49934:CDS |
| TAGTTTCTGTTCCTGTTCCT+AGG | 0.461589 | 6.4:+59261432 | None:intergenic |
| CACACGAAATCCACATATCC+TGG | 0.461613 | 6.4:-59245270 | MS.gene49934:CDS |
| TCTCAACATAGCCACTCATA+TGG | 0.469522 | 6.4:+59245331 | None:intergenic |
| AGGTTTGTTGAGCATTTAAG+AGG | 0.470542 | 6.4:-59246079 | MS.gene49934:CDS |
| CCAAAGGGGCTTCCATCATT+GGG | 0.472746 | 6.4:+59246034 | None:intergenic |
| GCCGCATGGAATTGATTGTC+AGG | 0.474919 | 6.4:-59244989 | MS.gene49934:CDS |
| CTAAAGAAGAATACTTCAAA+GGG | 0.474943 | 6.4:+59261273 | None:intergenic |
| AAGGAATGTGAAGAGGAAGC+AGG | 0.475425 | 6.4:-59244955 | MS.gene49934:CDS |
| TCATCTAGTTGCAGGAGGAC+TGG | 0.475914 | 6.4:-59245238 | MS.gene49934:intron |
| ACTAGATGATCAAGCATTCC+AGG | 0.480283 | 6.4:+59245252 | None:intergenic |
| AAATGAAGTTCCATTGTCCT+TGG | 0.485941 | 6.4:-59258438 | MS.gene49934:CDS |
| ACATGGAGAAGAAAATTAAA+GGG | 0.490149 | 6.4:-59258304 | MS.gene49934:CDS |
| ACGTTCTAATGAAAAGAAAA+CGG | 0.490303 | 6.4:+59245762 | None:intergenic |
| ACGTCGCCCTCAATGACAAA+TGG | 0.490482 | 6.4:+59246225 | None:intergenic |
| AATTAAAGGGAAAGTTACAT+TGG | 0.494880 | 6.4:-59258291 | MS.gene49934:CDS |
| TTGTCAAATGAGGACTTCAC+AGG | 0.500036 | 6.4:+59245787 | None:intergenic |
| AATGAAACACAGTGACCAAA+GGG | 0.500047 | 6.4:+59246019 | None:intergenic |
| TCTCTGAATTTGTTCACTGA+TGG | 0.500195 | 6.4:+59258479 | None:intergenic |
| TTTCATATATGGACATCGAC+GGG | 0.501956 | 6.4:-59244535 | MS.gene49934:CDS |
| TAGATGGGCAGAAGCACCTA+TGG | 0.503368 | 6.4:-59245308 | MS.gene49934:CDS |
| TTGTTGGATTCATTCACAAT+CGG | 0.508086 | 6.4:-59246203 | MS.gene49934:intron |
| CTCCTAAGAAGCTTAAGAAT+AGG | 0.508472 | 6.4:-59243722 | MS.gene49934:CDS |
| ATTTCTTCCATTTGTCATTG+AGG | 0.509275 | 6.4:-59246232 | MS.gene49934:CDS |
| AATACCTCGTTCCGTATACC+TGG | 0.509788 | 6.4:+59245917 | None:intergenic |
| TTATTTCTCCACAAATGTAG+TGG | 0.511610 | 6.4:-59258516 | MS.gene49934:CDS |
| ATATATGAAACAGCACCAAC+AGG | 0.512369 | 6.4:+59244548 | None:intergenic |
| GATCCTTGTTCTTATCTACA+AGG | 0.513032 | 6.4:-59249906 | MS.gene49934:CDS |
| TTGTCAGGAAAATGTTGTAA+AGG | 0.514029 | 6.4:-59244974 | MS.gene49934:CDS |
| TCGACGGGGATAGATACAAA+AGG | 0.523200 | 6.4:-59244520 | MS.gene49934:CDS |
| ATCAAGCATTCCAGGATATG+TGG | 0.524432 | 6.4:+59245260 | None:intergenic |
| TCTAAAGAAGAATACTTCAA+AGG | 0.526059 | 6.4:+59261272 | None:intergenic |
| AGCACATCAACTAGCTGAAG+TGG | 0.527501 | 6.4:+59258227 | None:intergenic |
| CCGAACTTACTTAATAGAGA+TGG | 0.527571 | 6.4:+59244921 | None:intergenic |
| TGGAACAGACCAATCAATAT+GGG | 0.528463 | 6.4:-59261390 | MS.gene49934:CDS |
| GAGTGTGCACGTGCTGCCAC+TGG | 0.531569 | 6.4:+59249983 | None:intergenic |
| TTGACTTCCTGTTTCGACAC+GGG | 0.535466 | 6.4:-59244241 | MS.gene49934:intron |
| GAGGTTTGAATAATTGTGCA+TGG | 0.540705 | 6.4:+59258362 | None:intergenic |
| TTGCTACTGTTGCAGCCGCA+TGG | 0.541001 | 6.4:-59245003 | MS.gene49934:intron |
| ATATAGTGTAGCTCACTACA+TGG | 0.546406 | 6.4:-59258321 | MS.gene49934:CDS |
| GCAGCACGTGCACACTCACA+TGG | 0.546676 | 6.4:-59249977 | MS.gene49934:CDS |
| TACTTAATAGAGATGGACCT+GGG | 0.564877 | 6.4:+59244928 | None:intergenic |
| GATTACATAGCCAAGCGCGC+TGG | 0.567603 | 6.4:+59245964 | None:intergenic |
| TGTGCACGTGCTGCCACTGG+TGG | 0.568995 | 6.4:+59249986 | None:intergenic |
| TCTTGCAGAGCTAAACCTGT+TGG | 0.576211 | 6.4:-59244563 | MS.gene49934:intron |
| AAGAGGAAGCAGGAATCCCC+AGG | 0.577108 | 6.4:-59244945 | MS.gene49934:CDS |
| CGACGGGGATAGATACAAAA+GGG | 0.578922 | 6.4:-59244519 | MS.gene49934:CDS |
| GAAAACGGGTTTGTCAAATG+AGG | 0.582709 | 6.4:+59245777 | None:intergenic |
| CAATGAAACACAGTGACCAA+AGG | 0.586554 | 6.4:+59246018 | None:intergenic |
| GACTTCTCTTCGCTATCCAT+AGG | 0.590285 | 6.4:+59245292 | None:intergenic |
| AAAGTCAAACTTTGCAACCG+TGG | 0.591041 | 6.4:-59249873 | MS.gene49934:CDS |
| ACAACCAATCATACCAGACA+TGG | 0.594195 | 6.4:+59261178 | None:intergenic |
| TTTCTGCAACTTGCTTAACA+GGG | 0.595814 | 6.4:+59244312 | None:intergenic |
| TGTAATCGAGCATTTGGGAG+AGG | 0.600564 | 6.4:-59245948 | MS.gene49934:CDS |
| TGTTGTAAAGGAATGTGAAG+AGG | 0.609830 | 6.4:-59244962 | MS.gene49934:CDS |
| GTTTCATATATGGACATCGA+CGG | 0.610300 | 6.4:-59244536 | MS.gene49934:CDS |
| ATTGACTTCCTGTTTCGACA+CGG | 0.610821 | 6.4:-59244242 | MS.gene49934:CDS |
| TGAAGTGGAGCAGTTAGTTG+AGG | 0.612568 | 6.4:+59258242 | None:intergenic |
| GTGGCTATGTTGAGATAGAT+GGG | 0.621537 | 6.4:-59245323 | MS.gene49934:CDS |
| TATTCAAACCTCTAGTGACA+TGG | 0.628354 | 6.4:-59258351 | MS.gene49934:CDS |
| GCTTATGGAGTCCATATGAG+TGG | 0.651417 | 6.4:-59245342 | MS.gene49934:CDS |
| TGGAACAACCATGTCACTAG+AGG | 0.653480 | 6.4:+59258343 | None:intergenic |
| TTACTTAATAGAGATGGACC+TGG | 0.657192 | 6.4:+59244927 | None:intergenic |
| CTTGATCATCTAGTTGCAGG+AGG | 0.661487 | 6.4:-59245243 | MS.gene49934:CDS |
| TCCTGACAATCAATTCCATG+CGG | 0.664669 | 6.4:+59244988 | None:intergenic |
| TCCATTGCTGAAGACAGCTG+AGG | 0.673124 | 6.4:-59245993 | MS.gene49934:CDS |
| CAGCACGTGCACACTCACAT+GGG | 0.680877 | 6.4:-59249976 | MS.gene49934:CDS |
| TAACCTTGTAGATAAGAACA+AGG | 0.682039 | 6.4:+59249903 | None:intergenic |
| TGTCAAATGAGGACTTCACA+GGG | 0.682552 | 6.4:+59245788 | None:intergenic |
| TTCATATATGGACATCGACG+GGG | 0.710863 | 6.4:-59244534 | MS.gene49934:CDS |
| ACTTAATAGAGATGGACCTG+GGG | 0.711401 | 6.4:+59244929 | None:intergenic |
| TATTCCAGGTATACGGAACG+AGG | 0.728323 | 6.4:-59245921 | MS.gene49934:intron |
| GTAAAGAACTCACAGAACCA+CGG | 0.729302 | 6.4:+59249856 | None:intergenic |
| ATGAAACACAGTGACCAAAG+GGG | 0.740460 | 6.4:+59246020 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 59243709 | 59261501 | 59243709 | ID=MS.gene49934 |
| chr6.4 | mRNA | 59243709 | 59261501 | 59243709 | ID=MS.gene49934.t1;Parent=MS.gene49934 |
| chr6.4 | exon | 59261192 | 59261501 | 59261192 | ID=MS.gene49934.t1.exon1;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59261192 | 59261501 | 59261192 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59258222 | 59258605 | 59258222 | ID=MS.gene49934.t1.exon2;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59258222 | 59258605 | 59258222 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59249869 | 59250008 | 59249869 | ID=MS.gene49934.t1.exon3;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59249869 | 59250008 | 59249869 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59246204 | 59246268 | 59246204 | ID=MS.gene49934.t1.exon4;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59246204 | 59246268 | 59246204 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59245922 | 59246099 | 59245922 | ID=MS.gene49934.t1.exon5;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59245922 | 59246099 | 59245922 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59245735 | 59245815 | 59245735 | ID=MS.gene49934.t1.exon6;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59245735 | 59245815 | 59245735 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59245239 | 59245364 | 59245239 | ID=MS.gene49934.t1.exon7;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59245239 | 59245364 | 59245239 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59244931 | 59245010 | 59244931 | ID=MS.gene49934.t1.exon8;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59244931 | 59245010 | 59244931 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59244462 | 59244577 | 59244462 | ID=MS.gene49934.t1.exon9;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59244462 | 59244577 | 59244462 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59244242 | 59244362 | 59244242 | ID=MS.gene49934.t1.exon10;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59244242 | 59244362 | 59244242 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| chr6.4 | exon | 59243709 | 59243778 | 59243709 | ID=MS.gene49934.t1.exon11;Parent=MS.gene49934.t1 |
| chr6.4 | CDS | 59243709 | 59243778 | 59243709 | ID=cds.MS.gene49934.t1;Parent=MS.gene49934.t1 |
| Gene Sequence |
| Protein sequence |
